Ciprofloxacin – Modified Degradable Hybrid Polyurethane-Polylactide Porous Scaffolds Developed for Potential Use as an Antibacterial Scaffold for Regeneration of Skin

The aim of performed studies was to fabricate an antibacterial and degradable scaffold that may be used in the field of skin regeneration. To reach the degradation criterion the biocompatible polyurethane (PUR), obtained by using amorphous macrodiol α,ω-dihydroxy(ethylene-butylene adipate) macrodiol (PEBA), was used and processed with so-called “fast-degradable” polymer polylactide (PLA) (5 wt% or 10 wt%). To meet the antibacterial requirement obtained hybrid PUR-PLA scaffolds (HPPS) were modified with ciprofloxacin (Cipro) (2 wt% or 5 wt%), the fluoroquinolone antibiotic inhibiting growth of bacteria such as Pseudomonas aeruginosa, Escherichia Coli and Staphylococcus aureus, which are main cause of wound infections. Obtained unmodified and Cipro-modified HPPS were studied towards their chemical composition to detect presence or absence of characteristic functional groups of PUR, PLA and Cipro, and as well to indicate the participation of hydrogen bonds in the HPPS structure in dependence on PLA addition and ciprofloxacin modification. Mechanical properties were studied to determine the possible application of HPPS as a skin tissue scaffold. Scanning electron microscopy (SEM) was used to study morphology of unmodified and Cipro-modified HPPS and to performed elementary analysis by using energy-dispersive x-ray spectroscopy (EDX) of obtained materials. Finally, the microbiological tests were performed to indicate the antibacterial effect of Cipro-modified HPPS on S.aureus growth. Performed studies showed that Cipro-modified HPPS, obtained by using 5 % of PLA, possess suitable mechanical characteristic, morphology, degradation rate and demanded antimicrobial properties to be further developed as a potential scaffolds for skin tissue engineering.

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Ciprofloxacin-Modified Degradable Hybrid Polyurethane-Polylactide Porous Scaffolds Developed for Potential Use as an Antibacterial Scaffold for Regeneration of Skin

Polymers ◽

10.3390/polym12010171 ◽

2020 ◽

Vol 12 (1) ◽

pp. 171 ◽

Cited By ~ 1

Author(s):

Carayon Iga ◽

Terebieniec Agata ◽

Łapiński Marcin ◽

Filipowicz Natalia ◽

Kucińska-Lipka Justyna

Keyword(s):

Mechanical Characteristics ◽

Antimicrobial Properties ◽

Porous Scaffolds ◽

Wound Infections ◽

Degradable Polymer ◽

Degradation Rates ◽

And Staphylococcus Aureus ◽

Regeneration Of Skin ◽

Potential Use ◽

Growth Of Bacteria

The aim of the performed study was to fabricate an antibacterial and degradable scaffold that may be used in the field of skin regeneration. To reach the degradation criterion for the biocompatible polyurethane (PUR), obtained by using amorphous α,ω-dihydroxy(ethylene-butylene adipate) macrodiol (PEBA), was used and processed with so-called “fast-degradable” polymer polylactide (PLA) (5 or 10 wt %). To meet the antibacterial requirement obtained, hybrid PUR-PLA scaffolds (HPPS) were modified with ciprofloxacin (Cipro) (2 or 5 wt %) and the fluoroquinolone antibiotic inhibiting growth of bacteria, such as Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus, which are the main causes of wound infections. Performed studies showed that Cipro-modified HPPS, obtained by using 5% of PLA, possess suitable mechanical characteristics, morphology, degradation rates, and demanded antimicrobial properties to be further developed as potential scaffolds for skin tissue engineering.

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Pectin-chitosan-PVA nanofibrous scaffold made by electrospinning and its potential use as a skin tissue scaffold

Journal of Biomaterials Science Polymer Edition ◽

10.1080/09205063.2012.693047 ◽

2012 ◽

Vol 24 (4) ◽

pp. 470-484 ◽

Cited By ~ 43

Author(s):

Hsin-Yi Lin ◽

Hsin-Hung Chen ◽

Shih-Hsin Chang ◽

Tsung-Sheng Ni

Keyword(s):

Nanofibrous Scaffold ◽

Skin Tissue ◽

Tissue Scaffold ◽

Potential Use

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Facile Bio-Fabrication of Ag-Cu-Co Trimetallic Nanoparticles and Its Fungicidal Activity against Candida auris

Journal of Fungi ◽

10.3390/jof7010062 ◽

2021 ◽

Vol 7 (1) ◽

pp. 62 ◽

Cited By ~ 1

Author(s):

Majid Rasool Kamli ◽

Vartika Srivastava ◽

Nahid H. Hajrah ◽

Jamal S. M. Sabir ◽

Khalid Rehman Hakeem ◽

...

Keyword(s):

Electron Microscopy ◽

Antimicrobial Properties ◽

Bloodstream Infections ◽

Multidrug Resistant ◽

Biologically Active ◽

Phase Cell ◽

Candida Auris ◽

Medical Problems ◽

One Pot ◽

X Ray

Candida auris is an emergent multidrug-resistant pathogen that can lead to severe bloodstream infections associated with high mortality rates, especially in hospitalized individuals suffering from serious medical problems. As Candida auris is often multidrug-resistant, there is a persistent demand for new antimycotic drugs with novel antifungal action mechanisms. Here, we reported the facile, one-pot, one-step biosynthesis of biologically active Ag-Cu-Co trimetallic nanoparticles using the aqueous extract of Salvia officinalis rich in polyphenols and flavonoids. These medicinally important phytochemicals act as a reducing agent and stabilize/capping in the nanoparticles’ fabrication process. Fourier Transform-Infrared, Scanning electron microscopy, Transmission Electron Microscopy, Energy dispersive X-Ray, X-ray powder diffraction and Thermogravimetric analysis (TGA) measurements were used to classify the as-synthesized nanoparticles. Moreover, we evaluated the antifungal mechanism of as-synthesized nanoparticles against different clinical isolates of C. auris. The minimum inhibitory concentrations and minimum fungicidal concentrations ranged from 0.39–0.78 μg/mL and 0.78–1.56 μg/mL. Cell count and viability assay further validated the fungicidal potential of Ag-Cu-Co trimetallic nanoparticles. The comprehensive analysis showed that these trimetallic nanoparticles could induce apoptosis and G2/M phase cell cycle arrest in C. auris. Furthermore, Ag-Cu-Co trimetallic nanoparticles exhibit enhanced antimicrobial properties compared to their monometallic counterparts attributed to the synergistic effect of Ag, Cu and Co present in the as-synthesized nanoparticles. Therefore, the present study suggests that the Ag-Cu-Co trimetallic nanoparticles hold the capacity to be a lead for antifungal drug development against C. auris infections.

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Evaluation of the Antimicrobial Protection of Pharmaceutical Kaolin and Talc Modified with Copper and Zinc

Materials ◽

10.3390/ma14051173 ◽

2021 ◽

Vol 14 (5) ◽

pp. 1173

Author(s):

Fotini Martsouka ◽

Konstantinos Papagiannopoulos ◽

Sophia Hatziantoniou ◽

Martin Barlog ◽

Giorgos Lagiopoulos ◽

...

Keyword(s):

Cation Exchange ◽

Photoelectron Spectroscopy ◽

Antimicrobial Properties ◽

Exchange Capacity ◽

Elevated Concentration ◽

Chemical Mapping ◽

Exchange Method ◽

X Ray ◽

Copper And Zinc ◽

Antimicrobial Protection

Six pharmaceutical pastes were prepared using chemically modified kaolin and talc powders. Tests were conducted to determine their structural and chemical characteristics as well as their antimicrobial protection, thus rendering them suitable for cosmetic and pharmaceutical uses. Kaolin and talc were treated chemically via the cation exchange method to load the clay particles with copper and zinc ions, two cations well known for their antimicrobial properties. Mineralogical analyses were conducted by using X-ray diffraction (XRD) before and after the modification, confirming the mineralogical purity of the samples. Scanning electron microscopy was also used in conjunction with energy dispersed spectroscopy (SEM-EDS) to obtain chemical mapping images, revealing the dispersion of the added metals upon the clay minerals surfaces. Moreover, chemical analysis has been performed (XRF) to validate the enrichment of the clays with each metal utilizing the cation exchange capacity. All modified samples showed the expected elevated concentration in copper or zinc in comparison to their unmodified versions. From the X-ray photoelectron spectroscopy (XPS), the chemical state of the samples’ surfaces was investigated, revealing the presence of salt compounds and indicating the oxidation state of adsorbed metals. Finally, the resistance of pastes in microbial growth when challenged with bacteria, molds, and yeasts was assessed. The evaluation is based on the European Pharmacopeia (EP) criteria.

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Sputter-Deposited Ag Nanoparticles on Electrospun PCL Scaffolds: Morphology, Wettability and Antibacterial Activity

Coatings ◽

10.3390/coatings11030345 ◽

2021 ◽

Vol 11 (3) ◽

pp. 345

Author(s):

Daniele Valerini ◽

Loredana Tammaro ◽

Roberta Vitali ◽

Gloria Guillot ◽

Antonio Rinaldi

Keyword(s):

Ag Nanoparticles ◽

Food Packaging ◽

Antimicrobial Agents ◽

Antimicrobial Properties ◽

Deposition Process ◽

Polymer Fibers ◽

Antibacterial Action ◽

Porous Scaffolds ◽

Electrospinning Technique ◽

Sputtering Deposition

Porous scaffolds made of biocompatible and environmental-friendly polymer fibers with diameters in the nano/micro range can find applications in a wide variety of sectors, spanning from the biomedical field to textiles and so on. Their development has received a boost in the last decades thanks to advances in the production methods, such as the electrospinning technique. Conferring antimicrobial properties to these fibrous structures is a primary requirement for many of their applications, but the addition of antimicrobial agents by wet methods can present a series of drawbacks. In this work, strong antibacterial action is successfully provided to electrospun polycaprolactone (PCL) scaffolds by silver (Ag) addition through a simple and flexible way, namely the sputtering deposition of silver onto the PCL fibers. SEM-EDS analyses demonstrate that the polymer fibers get coated by Ag nanoparticles without undergoing any alteration of their morphological integrity upon the deposition process. The influence on wettability is evaluated with polar (water) and non-polar (diiodomethane) liquids, evidencing that this coating method allows preserving the hydrophobic character of the PCL polymer. Excellent antibacterial action (reduction > 99.995% in 4 h) is demonstrated against Escherichia coli. The easy fabrication of these PCL-Ag mats can be applicable to the production of biomedical devices, bioremediation and antifouling systems in filtration, personal protective equipment (PPE), food packaging materials, etc.

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Silver Nanoparticles from Oregano Leaves’ Extracts as Antimicrobial Components for Non-Infected Hydrogel Contact Lenses

International Journal of Molecular Sciences ◽

10.3390/ijms22073539 ◽

2021 ◽

Vol 22 (7) ◽

pp. 3539

Author(s):

Anastasia Meretoudi ◽

Christina N. Banti ◽

Panagiotis K. Raptis ◽

Christina Papachristodoulou ◽

Nikolaos Kourkoumelis ◽

...

Keyword(s):

Silver Nanoparticles ◽

Contact Lenses ◽

Attenuated Total Reflection ◽

Bacterial Strains ◽

Scanning Calorimetry ◽

X Ray ◽

E Coli ◽

Polymer Hydrogels ◽

20 Nm ◽

And Staphylococcus Aureus

The oregano leaves’ extract (ORLE) was used for the formation of silver nanoparticles (AgNPs(ORLE)). ORLE and AgNPs(ORLE) (2 mg/mL) were dispersed in polymer hydrogels to give the pHEMA@ORLE_2 and pHEMA@AgNPs(ORLE)_2 using hydroxyethyl–methacrylate (HEMA). The materials were characterized by X-ray fluorescence (XRF) spectroscopy, X-ray powder diffraction analysis (XRPD), thermogravimetric differential thermal analysis (TG-DTA), derivative thermogravimetry/differential scanning calorimetry (DTG/DSC), ultraviolet (UV-Vis), and attenuated total reflection mode (ATR-FTIR) spectroscopies in solid state and UV–Vis in solution. The crystallite size value, analyzed with XRPD, was determined at 20 nm. The antimicrobial activity of the materials was investigated against Gram-negative bacterial strains Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E. coli). The Gram-positive ones of the genus of Staphylococcus epidermidis (S. epidermidis) and Staphylococcus aureus (S. aureus) are known to be involved in microbial keratitis by the means of inhibitory zone (IZ), minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). The IZs, which developed upon incubation of P. aeruginosa, E. coli, S. epidermidis, and S. aureus with paper discs soaked in 2 mg/mL of AgNPs(ORLE), were 11.7 ± 0.7, 13.5 ± 1.9, 12.7 ± 1.7, and 14.3 ± 1.7 mm. When the same dose of ORLE was administrated, the IZs were 10.2 ± 0.7, 9.2 ± 0.5, 9.0 ± 0.0, and 9.0 ± 0.0 mm. The percent of bacterial viability when they were incubated over the polymeric hydrogel discs of pHEMA@AgNPs(ORLE)_2 was interestingly low (66.5, 88.3, 77.7, and 59.6%, respectively, against of P. aeruginosa, E. coli, S. epidermidis, and S. aureus) and those of pHEMA@ORLE_2 were 89.3, 88.1, 92.8, and 84.6%, respectively. Consequently, pHEMA@AgNPs(ORLE)_2 could be an efficient candidate toward the development of non-infectious contact lenses.

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Investigation of silk sericin conformational structure for fabrication into porous scaffolds with poly(vinyl alcohol) for skin tissue reconstruction

European Polymer Journal ◽

10.1016/j.eurpolymj.2016.05.023 ◽

2016 ◽

Vol 81 ◽

pp. 43-52 ◽

Cited By ~ 6

Author(s):

M. Yooyod ◽

G.M. Ross ◽

N. Limpeanchob ◽

N. Suphrom ◽

S. Mahasaranon ◽

...

Keyword(s):

Vinyl Alcohol ◽

Skin Tissue ◽

Poly Vinyl Alcohol ◽

Porous Scaffolds ◽

Conformational Structure ◽

Silk Sericin ◽

Tissue Reconstruction

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Thermal analysis and X-ray diffraction of untreated coffee’s husk ash reject and its potential use in ceramics

Journal of Thermal Analysis and Calorimetry ◽

10.1007/s10973-012-2478-0 ◽

2012 ◽

Vol 111 (2) ◽

pp. 1331-1334 ◽

Cited By ~ 5

Author(s):

W. Acchar ◽

E. J. V. Dultra

Keyword(s):

Thermal Analysis ◽

X Ray Diffraction ◽

X Ray ◽

Potential Use

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Antimicrobial Activities of Coriander Seed Essential Oil and Silver Nanoparticles

10.21203/rs.3.rs-526332/v1 ◽

2021 ◽

Author(s):

Kadriye Ozlem saygi ◽

Birgul Kacmaz ◽

Serdar Gul

Keyword(s):

Silver Nanoparticles ◽

Essential Oil ◽

Bacterial Infections ◽

Materials Science ◽

Antimicrobial Properties ◽

Antimicrobial Activities ◽

Elemental Distribution ◽

Sem Images ◽

X Ray ◽

Coriander Seed

Abstract Bacterial infections are one of the most serious health problems all over the world, which cause need for the discovery of new drug. Since antibiotic resistance is a major threat to both humans and the environment, there is a need for studies on the antimicrobial properties of different forms of traditionally used plants. Herein, the seeds of coriander were used to isolate essential oil (EO) and to synthesise silver nanoparticles (C-AgNPs). The major oil constituents were characterized by GC-MS as Linalool (79.12%), Camphor (6.16%), γ-Terpinene (2.82%) and α-Pinene (2.67%). The synthesized AgNPs were characterized by UV-Visible spectrophotometry, Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX), X-ray difraction (XRD) and Fourier Tranmission Infrared (FTIR). The surface plasmon resonance (SPR) of C-AgNPs at 437 nm was recorded on the UV-Vis spectrometer. The spherical and homogenous of AgNPs was presented in SEM images. EDX showed elemental distribution and confirmed AgNPs. A characteristic intense peak was at 3.0 keV. The antibacterial activities of the essential oil and AgNPs form of coriander seed against Staphylococcus aureus ATCC 29213 and Escherichia coli ATCC 25922 bacteria were investigated by broth microdilution test. AgNPs and essential oil of coriander can be expected to provide future opportunities in nanomedicine and materials science. AgNPs can be displayed synergistic antimicrobial effect when used in combination with essential oil.

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