scholarly journals The protective effect of Paliasa (Kleinhovia hospita L.) leaf extract against elevated total bilirubin serum induced by toxic dose of antituberculosis in rats

2019 ◽  
Vol 4 (2) ◽  
pp. 43
Author(s):  
Yulia Yusrini Djabir
Keyword(s):  
Protective Effect ◽  
Liver Dysfunction ◽  
Leaf Extract ◽  
Total Bilirubin ◽  
Statistical Significance ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Group V ◽  
Toxic Dose ◽  
Group I

Liver dysfunction is implicated with the use of Antituberculosis (AT) leading to low compliance of TB patients to AT regimen. One of biomarker that is important to measure liver dysfunction is total bilirubin serum. Paliasa leaves have been emiprically used to improve liver function in South Sulawesi. This study aimed to determine the effect of ethanolic extract of Paliasa leaves on total bilirubin serum in rats. Twenty rats were devided into five groups: Group I served as the healthy control was only given NaCMC suspension, group II was treated with 178 mg/200gBB of AT suspension, while group III, group IV and group V  were given Paliasa extract 125 mg/kgBB, 250mg/kgBB and 500 mg/kgBB, respectively, 4 hours prior to AT suspension administration. Treatments were performed once a day for 28 days. Blood sampling was carried out 24 hours following the last treatment. The total bilirubin levels were measured using Humalyzer 3500. The results showed that the administrations of AT suspension for 28 days significantly increased rat total bilirubin levels. Paliasa leaf extract in all given dose was able to reduce the total bilirubin levels of rats compared to the group given only AT suspension. However, statistical significance was only reached by the groups that were treated with Paliasa extract 250 and 500 mg/kgBB. Therefore it is concluded that ethanol extract at a dose of 250 and 500 mg/kgBB has protective effect against AT-induced elevation of total bilirubin serum in rats.

scholarly journals Dimethylnitrosamine (DMN) pre-treated rats and protective effect of Vernonia amygdalina post-treatment on liver function

2016 ◽  
Vol 4 (1) ◽  
pp. 74
Author(s):  
Usunomena Usunobun ◽  
Gabriel Anyanwu
Keyword(s):  
Total Protein ◽  
Leaf Extract ◽  
Total Bilirubin ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Post Treatment ◽  
Vernonia Amygdalina ◽  
Male Wistar Rats ◽  
Drug Treatments ◽  
Ethanolic Leaf Extract

Background: Liver disease and toxicity is common, especially with many drug treatments. The effect of Vernonia amygdalina ethanolic leaf extract post-treatment on rats pre-treated with dimethylnitrosamine (DMN) was evaluated.Methods: Male wistar rats were orally administered DMN (single dose of 25 mg/kg) on first day and thereafter post-treated with 100 and 200mg/kg ethanolic leaf extract of Vernonia amygdalina for seven days. Analysis of serum concentrations of albumin, total protein, total bilirubin and glucose were carried out.Results: Administration of DMN alone to rats significantly increased glucose and total bilirubin concentration (P < 0.05) in the serum while it significantly reduced (P<0.05) serum total protein and albumin concentrations when compared with controls. However, post-treatment of DMN administered rats with 100 and 200 mg/kg ethanolic extract leaf of Vernonia amygdalina significantly (P<0.05) reversed these changes in a concentration dependent manner.Conclusion: In conclusion ethanolic leaf extract of Vernonia amygdalina have a potent hypoglycaemic and hepatoprotective action against dimethylnitrosamine-induced liver damage in rats may be due to its antioxidant properties.

scholarly journals FORMULATION AND EVALUATION OF PHARMACEUTICALY STABLE SERUM OF DUCHESNEA INDICA (ARBENAN)

2021 ◽  
Author(s):  
A. Mumtihanah Mursyid ◽  
Risda Waris
Keyword(s):  
Peer Review ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Initial Stage ◽  
Unique System ◽  
Duchesnea Indica ◽  
Leaf Powder ◽  
Review Reports ◽  
Stable Leaf

Objective: Arbenan (Duchesnea indica) plants contain saponins, flavonoids, and tannins which have antioxidant activity. The purpose of this research is to perform formulation and evaluation extract ethanol of Arbenan leaves in the form of serum which is pharmaceutically stable. Method: Arbenan leaf powder was macerated with ethanol solvent, and then left for 3-4 days while stirring repeatedly, and then filtering. Furthermore, the liquid ethanol extract that has been obtained is evaporated using a Rotary Vacum Evaporator was used to evaporate the extract. Prepared extract was used to evaluate various parameters like organoleptics, homogeneity, viscosity, and pH. Result: All formulations were having typical smell, light brown color and a little thick consistency. Formulations of leaf extract of Arabenan with four variations bases have shown to have good stability after stress condition. It can be seen from the evaluation result are organoleptics, homogeneity, viscosity, rheology, and pH. Conclusion: Study concludes that a stable leaf extract of Arabenan can be effectively formulated into a serum by the means of various bases.                                     Peer Review History: Received 8 January 2021; Revised 13 February; Accepted 4 March, Available online 15 March 2021 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency.  Received file:                Reviewer's Comments: Average Peer review marks at initial stage: 5.0/10 Average Peer review marks at publication stage: 7.0/10 Reviewer(s) detail: Dr. Marwa A. A. Fayed,  University of Sadat City, Egypt, [email protected] Prof. Dr. Ali Gamal Ahmed Al-kaf,  Sana'a university, Yemen, [email protected] Ahmad Najib, Universitas Muslim Indonesia, Makassar, Indonesia, [email protected] Similar Articles: PHYTOCHEMICAL, ANTI-INFLAMMATORY, ANALGESIC, ANTIPYRETIC AND ACUTE TOXICITY OF PSIADIA PUNCTULATA GROWING IN YEMEN ANTIHYPERLIPIDEMIC EFFECT OF THE ETHANOLIC EXTRACT OF SCAEVOLA TACCADA (GAERTN) ROXB. LEAVES ETHNOBOTANY, PHYTOCHEMISTRY AND PHARMACOLOGY OF OCHNA SCHWEINFURTHIANA: A REVIEW

scholarly journals INHIBITORY EFFECT OF ETHANOLIC EXTRACT OF ERIOBOTRYA JAPONICA LEAVES PRE-INCUBATED WITH THEOPHYLLINE AND ASPIRIN ON ISOLATED GUINEA PIG TRACHEAL

2018 ◽  
Vol 11 (13) ◽  
pp. 46
Author(s):  
Marianne Marianne ◽  
Urip Harahap ◽  
Emil Salim ◽  
Dadang Irfan Husori ◽  
Fahrumsyah Jali Rambe ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Protective Effect ◽  
Contractile Response ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Eriobotrya Japonica ◽  
Relaxant Effect ◽  
Significant Difference

 Objectives: The objectives of the study were to examine the inhibitory effect of ethanol extract of Eriobotrya japonica leaves (EEEJL) pre-incubated with theophylline and aspirin on isolated guinea pig tracheal chains against acetylcholine (ACh)-induced contraction.Methods: The effect of EEEJL (1-8 mg/Ml) on the isolated tracheal strips was tested in vitro. Furthermore, the mechanism of relaxant effects of EEEJL was evaluated in the presence of theophylline and aspirin.Results: The contractile response evoked by Ach (1.25 × 10−3 M) was decreased by EEEJL (effective concentration50 = 1.36 mg/mL) and has no significant difference of relaxant effect to that of EEEJL pre-incubated with theophylline and aspirin (p>0.05).Conclusion: The EEEJL decreased the ACh-induced contraction through the inhibition of PDE and the protective effect on prostaglandin E2.

scholarly journals Nephrotherapy Determination of Ethanol Extract of African Leaves (Vernonia amygdalina Delile) on with the Parameter of Male Rat Urea Induced by Gentamisin

2019 ◽  
pp. 162-169
Author(s):  
Rizqi Nur Azizah ◽  
Irma Santi ◽  
Ayu Marlian
Keyword(s):  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Negative Control ◽  
Group Iv ◽  
Male Rat ◽  
Vernonia Amygdalina ◽  
Group I ◽  
Supplement Group ◽  
Post Hoc

African leaves (Vernonia amygdalina Delile.) contain flavonoids having an antioxidant activities. The research aimed to determine the nephrotherapy effect of the ethanolic extract of African leaves with the parameter of male rat urea (Rattus norvegicus) induced by gentamicin. The research used 18 rats divided into 6 groups consisting of group I as the normal group, group Il the Negative Control (gentamicin 80 mg I kgBW), group Ill the Positive Contfol (amino acid supplement), group IV, V, and VI given the ethanol extract of African leaves, each dose of 100 mg/kgBW, 250 mg/kgBW, 500 mg/kgBW. The induction of gentamicin 80 mg/kg BW was administered for eight days and the therapy for the ethanol extract of African leaves for fourteen days. The research data were statistically analyzed using the ANOVA test followed by the Post Hoc LSD test. The results showed that the ethanol extract of African leaves had the nephrotherapy effects in reducing the urea in male rat induced by gentamicin.

scholarly journals Protective effect of Berberis asiatica root on biochemical and histopathological changes in streptozotocin-induced diabetic Wistar rats

2017 ◽  
Vol 6 (8) ◽  
pp. 1880 ◽  
Author(s):  
Mohd Muddassir Husain Khan ◽  
Chetan Rastogi ◽  
Sachin Gupta ◽  
Shravan Kumar Paswan ◽  
Pritt Verma ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Liver Function ◽  
Blood Glucose Level ◽  
Protective Effect ◽  
Glucose Level ◽  
Wistar Rats ◽  
Total Bilirubin ◽  
Group Iv ◽  
Group Iii ◽  
Group I

Background: This study was designed to evaluate the effect of Berberis asiatica root extract (BAE) against streptozotocin induced elevated blood glucose level and other liver and kidney functions changes in adult male Wistar rats.Methods: Thirty male Wistar rats were divided into five groups of six rats in each (Groups I-V). Group I and Group II served as normal control and disease control, respectively. Group III received standard anti-diabetic drug glibenclamide (5mg/kg), while Group IV and Group V received the low dose (250mg/kg) and high dose (500mg/kg) of BAE. Serum blood glucose, SGOT, SGPT, ALP, total bilirubin, BUN, serum creatinine, TC, TG, HDL-C, LDL-C, and VLDL-C were estimated using standard methods. After collection of samples for biochemical evaluation, the pancreas from each animal was isolated and examined for histological changes.Results: BAE and glibenclamide treated disease rats showed significant (p <0.05) decrease in blood glucose concentration. Treatment with BAE at 250mg/kg and 500mg/kg in Group IV and V and standard drug glibenclamide in Group III showed significant (p <0.05) reduction in the level of liver function substances such as SGOT, SGPT, ALP and total bilirubin as compared to disease group, as well as showed significant (p <0.05) decrease in renal panel. Liver function parameters were significantly (p <0.05) improved in groups treated with BAE. Histopathological analysis revealed the protective effect of BAE against streptozotocin induced damage to islets of Langerhans.Conclusions: This study showed the destruction of islets of Langerhans and elevation in blood glucose level as well as alteration in other biochemical parameters were ameliorated by the effect of Berberis asiatica extract.

scholarly journals Antigenotoxic Activity of Rumput Mutiara (Hedyotis corymbosa L.) Ethanolic Extract on Cyclophosphamide-Induced Mice

2018 ◽  
Vol 8 (3) ◽  
pp. 135
Author(s):  
Yoce Aprianto ◽  
Asri Mega Putri ◽  
Hilyatul Fadliyah ◽  
Retno Murwanti ◽  
Edy Meiyanto
Keyword(s):  
Molecular Docking ◽  
Ursolic Acid ◽  
Micronucleus Test ◽  
Ethanolic Extract ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Adult Mice ◽  
Group I

Exposure to relative chemicals has been shown to induce a genotoxic effect that can be observed through formation of micronucleus (MN) in polychromatic erythrocythes (PCE). Rumput Mutiara or Hedyotis corymbosa L. ethanolic extract (HcEE) is known to contain ursolic acid as major compound that possesses antigenotoxic activity on HepG2 cells. This study exerts in vivo approach aiming to evaluate the antigenotoxic effects of HcEE on cyclophosphamide (CP)-induced male Swiss mice. The ursolic acid on HcEE was determined by using thin layer chromatography with silica gel as stationary phase and chloroform-aceton (9:1) as mobile phase. The antigenotoxic activity was carried out by in vivo micronucleus test. Twenty four adult mice were equally divided into seven groups. Group I: control (untreated); group II: Na-CMC 0.5%; group III: CP 50 mg/kg BW; group IV: CP+HcEE 250 mg/kg BW; group V: CP+HcEE 500 mg/kg BW; group VI: CP+HcEE 1000 mg/kg BW; group VII: HcEE 1000 mg/kg BW. HcEE were given for seven days, while CP was administered on the last two days. On the seventh day, the peripheral blood from all mice were collected, smeared, and then stained with Giemsa. The frequencies of MNPCEs and %PCEs were evaluated. Molecular docking was performed to know the interaction between ursolic acid and CYP3A4 by using PLANTS software. There was similar hRF spot between HcEE with ursolic acid standard reference indicated that the extract almost positively contain ursolic acid. HcEE reduced MNPCEs significantly compared to CP group (p<0.05) and combination of CP with HcEE showed reduction of %PCEs (p<0.05). Based on molecular docking analysis, ursolic acid gave lower docking score than CP against CYP3A4 (PDB ID: 2V0M) and similar binding site on amino acid residues Ala 448, Ile 369, Thr 309, and Val 313. All of these data suggest that HcEE perform protective effect against CP-induced genotoxicity.Keywords: Antigenotoxic, Hedyotis corymbosa L., cyclophosphamide, micronucleus, molecular docking

scholarly journals A Histopathological Study of Cisplatin-induced Acute Vascular Injuries in Vital Organs and Protective Effect of Achillea millefolium

2020 ◽  
pp. 56-69
Author(s):  
Zahra Eslamifar ◽  
Susan Sabbagh
Keyword(s):  
Single Dose ◽  
Protective Effect ◽  
Wistar Rats ◽  
Ethanolic Extract ◽  
Achillea Millefolium ◽  
Control Group ◽  
Histopathological Study ◽  
Histopathological Analysis ◽  
Vascular Injuries ◽  
Group I

The aim was to study the protective effect of ethanolic extract of Achillea millefolium on acute vascular injuries induced by cisplatin in liver, heart and renal tissues 24 hour after administration and using histopathological surveys in wistar rats. 24 adult male wistar rats were randomly divided into four groups. Group I (control group) received physiological saline for 10 days. Animals of group II had single dose of injection of CP (cisplatin) (6 mg/kg, IP) on the ninth day. Group III received Achillea millefolium extract (250 mg/kg, gavage) for 10 consecutive days. Group IV had both Achillea millefolium extract (250 mg/kg, gavage) for 10 consecutive days and a single dose of injection of CP (6 mg/kg, IP) on the ninth day. Kidney, liver and heart organs were collected on 10th day from sacrificed rats and subjected to histopathological analysis. Then the possible histopathological vascular effects of cisplatin on liver, heart, kidney tissues and the protective effect of Achillea millefolium extract was analysed. Obtained data showed the vascular injuries in CP group as congestion of cardiac capillaries (p=0.00) and interstitial edema (p=0.03). In the kidney, shrinkage of glomeruli (p=0.04), widening of Bowman's space (p=0.04), dilatation of cortical capillaries (p=0.01) were significantly altered. The findings of liver organ were increased sinusoidal space (p=0.00) and infiltration of neutrophils in portal space (p=0.01). Pretreatment with ethanolic extract of Achillea millefolium could attenuate these vascular injuries. Briefly, 24 hour after single injection of cisplatin the inflammatory process was seen in vital organs and administration of Achillea millefolium could mitigate these side effects.

scholarly journals Effects of Binahong (Anredera cordifolia (Tenore) Steenis) Extracts on the Levels of Malondialdehyde (MDA) in Cataract Goat Lenses

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Feriyani Feriyani ◽  
Hady Maulanza ◽  
Rodiah Rahmawaty Lubis ◽  
Ummu Balqis ◽  
Darmawi Darmawi
Keyword(s):  
Oxidative Stress ◽  
Leaf Extract ◽  
Positive Control ◽  
Group Iv ◽  
Diabetic Cataract ◽  
Group V ◽  
Group Iii ◽  
Pathological Conditions ◽  
Group I ◽  
High Oxidative Stress

Cataracts are one of the most causes of blindness in the world. Oxidative stress can form pathological conditions such as cataracts. This oxidative stress ability can be measured by the malondialdehyde (MDA) biomarker. Binahong leaves (Anredera cordifolia (Tenore) Steenis) are native plants from Indonesia that are used to treat various diseases including cataract treatment. Binahong leaf (Anredera cordifolia (Tenore) Steenis) has a high amount of flavonoids and is rich in antioxidants that can be used to treat cataracts. Objective. The purpose of this study was to assess the effect of binahong leaf extract on the levels of MDA in a goat lens with cataract-induced material. Method. As many as possible, 40 goat eye lenses were divided into several groups, namely, group I normal lenses as controls (glucose 5.5 mM), group II lenses were cataract induced with glucose concentration of 55 mM, group III lenses with glucose 55 mM + binahong leaf extract (100 μg/ml), group IV lens with glucose 55 mM + binahong leaf extract (200 μg/ml), and group V lens with glucose 55 mM + quercetin (positive control). Biochemical parameters measured in the lens homogenate are malondialdehyde lens morphology in all groups’ observations and comparisons made. Results. The results of the study found that the lens group with the addition of binahong extract showed more results transparency compared to lens groups induced by glucose concentrations of 55 mM). This shows that the diabetic cataract group experienced high oxidative stress due to the accumulation of sorbitol compounds derived from glucose which caused turbidity in the goat eye lens and increased levels of lens MDA. Binahong levels at concentrations of 100 or 200 can inhibit MDA production. Conclusion. Binahong (Anredera cordifolia (Tenore) Steenis) extract has the ability to inhibit the production of MDA levels. In glucose-induced goat lenses, binahong extract and quercetin show antioxidant and anticataract properties.

scholarly journals Evaluation of protective effect of hydroalcoholic extract of saffron petals in prevention of acetaminophen-induced renal damages in rats

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Arash Omidi ◽  
Narges Riahinia ◽  
Mohammad Bagher Montazer Torbati ◽  
Mohammad Ali Behdani
Keyword(s):  
Uric Acid ◽  
Serum Creatinine ◽  
Protective Effect ◽  
Ethanolic Extract ◽  
Control Group ◽  
Pathologic Evaluation ◽  
Treatment And Prevention ◽  
Group I ◽  
Male Wistar Rats ◽  
The Right

In recent years more attention has been given to herbal drugs in the treatment and prevention of drug toxicity because of the harmful effects of chemical drugs. In this study, directed for this purpose, research was conducted on the protective effect of hydro-ethanolic extract of saffron petals (SPE) against acetaminophen (APAP) induced acute nephrotoxicity. Twenty-four male Wistar rats were distributed into four groups of six each. Group I, as a control group, received normal saline (0.09%) orally (PO). Group II, as an intoxicated group was treated with APAP, PO (600 mg/kg). In the groups III and IV, SPE in a dose of 10 and 20 mg/kg along with APAP (600 mg/kg) was administered, respectively. At the end of the trial (8th day), blood was taken from the heart of rats for assessment of biochemical parameters and the right kidney was placed in 10% buffered formalin for histopathological evaluations. In the APAP treatment group, higher serum creatinine and uric acid were observed. SPE in a dose of 20 mg/kg significantly reduced serum creatinine and uric acid. In pathologic evaluation, a dose of 20 mg/kg of SPE prevented the kidney injuries induced by APAP. Tissues changes were in accordance with biochemical findings. It is likely that the SPE contributed to the prevention of acute nephrotoxicity induced by APAP.

In- vitro Antioxidant and Cardio-protective effect of Delonix elata (L.) Leaf extract against Doxorubicin induced toxicity in H9c2 Cardio-myocyte cell line

2021 ◽  
pp. 5635-5641
Author(s):  
Archana V ◽  
Indumathy R
Keyword(s):  
Reactive Oxygen Species ◽  
Protective Effect ◽  
Cell Viability ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Reactive Oxygen ◽  
Ethanolic Extract ◽  
Antioxidant Property ◽  
Oxygen Species

Objective: The aim of this study is to evaluate the protective effect of Delonix elata (L.) leaf extract against doxorubicin-induced cardiotoxicity in H9c2 cells. Methods: Doxorubicin has been used to treat cancer, but its clinical uses are limited because of its dose-dependent cardiotoxicity. Reactive oxygen species play an important role in the pathological process of cardiotoxicity. The various extracts (pet.ether, ethyl acetate and ethanol) of Delonix elata leaves antioxidant property was evaluated by SOD antioxidant assay and DPPH free radical scavenging assay. The cells were incubated with different concentrations of various extracts of Delonix elata leaves for 2 hr, followed by incubation with 5µM doxorubicin for 24 hr. Cell viability was determined by using MTT assay, respectively. Results: The various extracts of Delonix elata leaves exhibits antioxidant activity. The Doxorubicin significantly decreased cell viability which was accompanied by an increased ROS production. Pre-treatment with various extracts of Delonix elata leaves increased the viability ofcells and inhibit the generation of reactive oxygen species. Conclusion: In this study, findings how that Delonix elata leaf extract exhibited a protective effect against oxidative stress-induced cardiomyocyte damage. The ethanolic extract of Delonix elata leaves possesses significant antioxidant and cardioprotective activity.

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