ANTIOXIDANT AND HYPOGLYCEMIC EFFECTS OF WATERCRESS (NASTURTIUM OFFICINALE) EXTRACTS IN DIABETIC RATS

Background: Watercress is a semi-aquatic plant used in traditional medicine to treat various ailments, such as flu, cough, avitaminosis, and anorexia; it is also used as a diuretic and for hypoglycemia treatment in diabetes. In this study, we report the antioxidant and hypoglycemic activity of orally administered aqueous (WAQE), acetonic (WAE), and alcoholic (WOHE) watercress extracts. The effect of subchronic administration of watercress extracts on oxidative stress was also studied. Materials and Methods: WAQE, WAE, and WOHE were obtained and administered orally. Alloxan (200 mg/kg) and streptozotocin (60 mg/kg) were applied to induce hyperglycemia in male Wistar rats. Phenolic and flavonoid content, as well as antioxidant activity of the extracts were measured. The acute and subchronic effects (8 weeks) of WAQE were evaluated. The activity of antioxidant enzymes levels of malondialdehyde, hepatic enzyme markers in the serum, and renal function markers, were assessed. Histopathological evaluation of the pancreas, kidney, and liver was performed using hematoxylin-eosin staining. Results: Watercress extracts have high concentrations of phenols, polyphenols, and flavonoids, in addition to a very high antioxidant effect. The hypoglycemic effect of WAQE upon acute administration was 76.6% higher than that of insulin. When administered chronically, glucose levels were normalized on the third week up to the eighth week. Furthermore, the antioxidant enzymes and biochemical parameters improved. Conclusion: WAQE administration to diabetic rats reduced oxidative stress damage and decreased glucose levels. This study supports the use of this plant for the treatment of diabetes.

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Effects of Apocynin on Heart Muscle Oxidative Stress of Rats with Experimental Diabetes: Implications for Mitochondria

Antioxidants ◽

10.3390/antiox10030335 ◽

2021 ◽

Vol 10 (3) ◽

pp. 335

Author(s):

Estefanía Bravo-Sánchez ◽

Donovan Peña-Montes ◽

Sarai Sánchez-Duarte ◽

Alfredo Saavedra-Molina ◽

Elizabeth Sánchez-Duarte ◽

...

Keyword(s):

Oxidative Stress ◽

Insulin Sensitivity ◽

Cardiac Tissue ◽

Diabetic Rats ◽

Oxidase Inhibitor ◽

Beneficial Effects ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Transport Chain ◽

Male Wistar Rats

Diabetes mellitus (DM) constitutes one of the public health problems today. It is characterized by hyperglycemia through a defect in the β-cells function and/or decreased insulin sensitivity. Apocynin has been tasted acting directly as an NADPH oxidase inhibitor and reactive oxygen species (ROS) scavenger, exhibiting beneficial effects against diabetic complications. Hence, the present study’s goal was to dissect the possible mechanisms by which apocynin could mediate its cardioprotective effect against DM-induced oxidative stress. Male Wistar rats were assigned into 4 groups: Control (C), control + apocynin (C+A), diabetes (D), diabetes + apocynin (D+A). DM was induced with streptozotocin. Apocynin treatment (3 mg/kg/day) was applied for 5 weeks. Treatment significantly decreased blood glucose levels and insulin resistance in diabetic rats. In cardiac tissue, ROS levels were higher, and catalase enzyme activity was reduced in the D group compared to the C group; the apocynin treatment significantly attenuated these responses. In heart mitochondria, Complexes I and II of the electron transport chain (ETC) were significantly enhanced in the D+A group. Total glutathione, the level of reduced glutathione (GSH) and the GSH/ oxidized glutathione (GSSG) ratio were increased in the D+A group. Superoxide dismutase (SOD) and the glutathione peroxidase (GSH-Px) activities were without change. Apocynin enhances glucose uptake and insulin sensitivity, preserving the antioxidant defense and mitochondrial function.

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Modulation of Renal Insulin Signaling Pathway and Antioxidant Enzymes with Diabetes: Effects of Resveratrol

10.20944/preprints201810.0488.v1 ◽

2018 ◽

Author(s):

Gökhan Sadi ◽

Gamze Şahin ◽

Aykut Bostancı

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Signaling Pathway ◽

Insulin Signaling ◽

Diabetic Rats ◽

Insulin Signaling Pathway ◽

Inflammatory Agent ◽

Male Wistar Rats ◽

The Relationship ◽

Control State

Diabetes mellitus, a disease arising by the deficiency of insulin hormone or its inability of usage, affects carbohydrate, lipid and protein metabolism, and destruct variety of the tissues. A strong antioxidant and anti-inflammatory agent; resveratrol has a high potential to prevent or treat the pathogenesis of diseases. This study was conducted to reveal the relationship between diabetes-induced oxidative stress and tissue inflammation with changes in antioxidant enzymes (cat, sod, gpx, and gst) and the components of insulin signaling pathway (insulin Rβ, irs-1, pi3k, akt, mtor) in kidney tissues. Additionally, the effects of resveratrol on these parameters were evaluated. Male Wistar rats were randomly divided into four groups; (1) control/vehicle; (2) control/20 mg/kg resveratrol; (3) diabetic/vehicle; (4) diabetic/20 mg/kg resveratrol. Results demonstrated down-regulation of antioxidant enzymes in the kidney tissues of diabetic rats and this situation was devoted partially to the reduced expression of nfκb. Moreover, the components of renal insulin signaling elements were up-regulated in diabetic rats, and resveratrol treatment decreased this sensitization towards the control state. In conclusion, resveratrol improved diabetes-induced renal oxidative stress and inflammation partly due to healing action on renal antioxidant enzymes and insulin signaling pathway components.

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Modulation of Renal Insulin Signaling Pathway and Antioxidant Enzymes with Streptozotocin-Induced Diabetes: Effects of Resveratrol

Medicina ◽

10.3390/medicina55010003 ◽

2018 ◽

Vol 55 (1) ◽

pp. 3 ◽

Cited By ~ 3

Author(s):

Gökhan Sadi ◽

Gamze Şahin ◽

Aykut Bostanci

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Antioxidant Enzymes ◽

Signaling Pathway ◽

Insulin Signaling ◽

Diabetic Rats ◽

Insulin Signaling Pathway ◽

Enzymatic Antioxidants ◽

Gene And Protein Expression ◽

Male Wistar Rats

Background and objectives: Diabetes mellitus is a disease of insulin deficiency or its inability of usage by the target tissues leading to impairment of carbohydrate, lipid, and protein metabolisms. Resveratrol, having robust anti-inflammatory and anti-oxidant properties, has a high potential to treat or prevent the pathogenesis of diseases. This study was conducted to reveal the relationship between diabetes-induced oxidative stress and tissue inflammation with changes in main enzymatic antioxidants (cat, sod, gpx, and gst) and the components of the insulin signaling pathway (insulin Rβ, irs-1, pi3k, akt, mtor) in kidney tissues. Additionally, the effects of resveratrol on these parameters were evaluated. Materials and Methods: Male Wistar rats were randomly divided into four groups; (1) control/vehicle; (2) control/20 mg/kg resveratrol; (3) diabetic/vehicle; (4) diabetic/20 mg/kg resveratrol. Gene and protein expressions of antioxidant enzymes and insulin signaling elements were evaluated in renal tissues. Results: Downregulation of antioxidant enzymes’ gene expression in the kidney tissues of diabetic rats was demonstrated and this situation was devoted partially to the reduced gene expression of nfκb. Moreover, the components of renal insulin signaling elements were upregulated at both gene and protein expression levels in diabetic rats, and resveratrol treatment decreased this sensitization towards the control state. Conclusion: Resveratrol partially improved diabetes-induced renal oxidative stress and inflammation due to healing action on renal antioxidant enzymes and insulin signaling pathway components.

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Antihyperglycemic, Antidiabetic, and Antioxidant Effects of Garcinia pedunculata in Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2017/2979760 ◽

2017 ◽

Vol 2017 ◽

pp. 1-15 ◽

Cited By ~ 8

Author(s):

Md. Yousuf Ali ◽

Sudip Paul ◽

E. M. Tanvir ◽

Md. Sakib Hossen ◽

Nur-E Noushin Rumpa ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Enzymes ◽

Wistar Rats ◽

Diabetic Rats ◽

Normal Male ◽

Insulin Levels ◽

Nondiabetic Control ◽

Male Wistar Rats ◽

Treatment Of Diabetes ◽

Altered Lipid

The antihyperglycemic, antidiabetic, and antioxidant potentials of the methanolic extract of Garcinia pedunculata (GP) fruit in rats were investigated. The acute antihyperglycemic effect of different doses of GP was studied in normal male Wistar rats. Diabetes was induced by streptozotocin (STZ) injection in another cohort of male Wistar rats and they showed significantly higher blood glucose and glycated hemoglobin (HbA1c) levels, altered lipid profiles, and lower insulin levels compared to nondiabetic control animals. There were increased lipid peroxidation and reduced levels of cellular antioxidant enzymes in different tissues of diabetic rats. However, oral administration of GP extracts, especially the highest dose (1000 mg/kg), significantly ameliorated hyperglycemia (42%); elevated insulin levels (165%); decreased HbA1c (29.4%); restored lipid levels (reduction in TG by 25%, TC by 15%, and LDL-C by 75% and increase in HDL-C by 4%), liver and renal function markers, and lipid peroxidation (reduction by 52% in the liver, 39% in the kidney, 44% in the heart, and 46% in the pancreas); and stimulated tissue antioxidant enzymes to near normalcy. Overall, the findings suggest that GP fruit is effective against hyperglycemia and could be used in the treatment of diabetes and its complications and other oxidative stress-mediated pathological conditions.

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Modulation of Renal Insulin Signaling Pathway and Antioxidant Enzymes with Streptozotocin-Induced Diabetes: Effects of Resveratrol

10.20944/preprints201810.0488.v2 ◽

2019 ◽

Author(s):

Gökhan Sadi ◽

Gamze Şahin ◽

Aykut Bostancı

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Antioxidant Enzymes ◽

Signaling Pathway ◽

Insulin Signaling ◽

Diabetic Rats ◽

Insulin Signaling Pathway ◽

Enzymatic Antioxidants ◽

Gene And Protein Expression ◽

Male Wistar Rats

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Amelioration of Diabetes-Induced Nephropathy by Loranthus regularis: Implication of Oxidative Stress, Inflammation and Hyperlipidaemia

Applied Sciences ◽

10.3390/app11104548 ◽

2021 ◽

Vol 11 (10) ◽

pp. 4548

Author(s):

Ahmed Z. Alanazi ◽

Mohamed Mohany ◽

Fawaz Alasmari ◽

Ramzi A. A. Mothana ◽

Abdulaziz O. A. Alshehri ◽

...

Keyword(s):

Oxidative Stress ◽

Renal Tissue ◽

Serum Glucose ◽

Diabetic Rats ◽

Glucose Levels ◽

Renal Markers ◽

Male Wistar Rats ◽

Biochemical Indices ◽

Elevated Lipid

In traditional Yemeni medicine, various preparations of Loranthus regularis (L. regularis), such as powder, decoctions and infusions are commonly used to treat diabetes, kidney stone formations and inflammation. In the present study, we evaluated the antinephrotoxic effects of L. regularis extract in experimentally-induced diabetes in male Wistar rats. A single dose (60 mg/kg/day) of Streptozotocin (STZ) was used to induce type 1 diabetes. Animals were then treated for four weeks with L. regularis extract (150 or 300 mg/kg/day) by oral gavage. Renal and blood samples were subsequently harvested. Several biochemical indices, oxidative stress and inflammatory markers were assessed. Additionally, histological alterations in the renal tissue were examined. Serum glucose levels were significantly (p < 0.01) lowered while insulin levels were enhanced in L. regularis-treated diabetic animals. The increased renal markers in diabetic rats were decreased by L. regularis treatment. Serum elevated lipid profiles were markedly decreased by the plant extract. The serum and renal cytokines that were significantly increased (p < 0.001) by STZ were diminished by L. regularis treatment. Finally, renal tissue antioxidant enzymatic activity was enhanced with L. regularis treatment. Taken together, the data here indicate that L. regularis possesses therapeutic ability to reduce the development of diabetic nephropathy (DN) by minimizing oxidative injury and inflammation.

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Protective effects of Trigonella foenum-graecum crude extract over damage induced by Sreptozotocin diabetes rats

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v10i4-s.4256 ◽

2020 ◽

Vol 10 (4-s) ◽

pp. 138-141

Author(s):

Dalila Bencheikh ◽

Seddik Khennouf ◽

Saliha Dahamna

Keyword(s):

Streptozotocin Diabetes ◽

Diabetic Rats ◽

Antidiabetic Activity ◽

Protective Effects ◽

Traditional Use ◽

Orogastric Tube ◽

Trigonella Foenum Graecum ◽

Male Wistar Rats ◽

Treatment Of Diabetes

The seeds of Trigonella foenum-graecum (fenugreek) are used for treatment of diabetes mellitus in traditional medicine. This paper examines the protection effects of fenugreek from the damage induced by streptozotocin diabetes rats. Tannins content of T. foenum-graecum was also estimated in vitro. Normoglycemic male Wistar rats, weighing 170-250 g, were selected and randomly divided into five groups (n= 6): normal control, diabetic + TFGE (200mg/kg), diabetic+ TFGE (600mg/kg), diabetic + Glibil (3mg/kg), untreated group. Diabetes was induced after a single intraperitoneal injection of streptozotocin (50 mg/kg body weight) and Fenugreek was given every day via orogastric tube for 18 days. At the end of experiment, rats were sacarificed. Organ weight was estimated of all groups. Trigonella foenum-graecum administration significantly improved the polydipsia, polyphagia, and it also compensated weight loss of diabetic rats (P<0.05, P < 0.01). Moreover, fenugreek had a significant concentration of tannins (806.22 ±0.036 µg TAE/gE). The results revealed that fenugreek improves the damage in diabetic rats that in some ways validates the traditional use of this plant in treatment of diabetes. Keywords: Antidiabetic activity, Protective effect, Streptozotocin, Tannins, Trigonella foenum-graecum

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STREPTOZOTOCIN-INDUCED OXIDATIVE STRESS IN DIABETIC RATS - A DEFENSIVE EFFECT OF PSYDRAX DICOCCOS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i11.28019 ◽

2018 ◽

Vol 11 (11) ◽

pp. 378

Author(s):

Ravi Kumar V ◽

Sailaja Rao P

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Blood Glucose ◽

Methanolic Extract ◽

Antioxidant Effect ◽

Control Group ◽

Standard Drug ◽

Antihyperglycemic Activity ◽

Glucose Levels ◽

Blood Glucose Levels

Objective: The present study was aimed to evaluate the antihyperglycemic activity and in vivo antioxidant effect of methanolic extract of whole plant of Psydrax dicoccos (MEPD) belonging to the family Rubiaceae.Methods: MEPD was prepared by Soxhlet extraction. Wistar rats weighing (180–200 g) were divided into six groups (n=6), with three doses of 100 mg/kg, 200 mg/kg, and 400 mg/kg of extract. Metformin was used as a standard drug. Diabetes was induced by streptozotocin (STZ) (40–50 mg/kg, i.p) in control group. The animals were treated with different doses of extracts for 21 days, and on the 22nd day, the blood glucose levels along with antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and lipid peroxidase (LPO) were determined.Results: The phytochemical screening of the extract showed the presence of carbohydrates, phenolics, flavonoids, glycosides, and tannins. The methanolic extract of MEPD at the dose of 200 mg/kg body weight showed a significant reduction in blood glucose levels (**p<0.001) with the value of 151.2 mg/dl on the 22nd day at 8 h. A promising antioxidant effect was also evident from the determination of antioxidant enzymes such as SOD, CAT, and LPO.Conclusion: The P. dicoccos extract revealed a potential effect of antihyperglycemic activity and combating nature on oxidative stress induced by STZ.

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Electrospun Nanofibers Loaded with Quercetin Promote the Recovery of Focal Entrapment Neuropathy in a Rat Model of Streptozotocin-Induced Diabetes

BioMed Research International ◽

10.1155/2017/2017493 ◽

2017 ◽

Vol 2017 ◽

pp. 1-12 ◽

Cited By ~ 10

Author(s):

Chonlathip Thipkaew ◽

Jintanaporn Wattanathorn ◽

Supaporn Muchimapura

Keyword(s):

Oxidative Stress ◽

Functional Recovery ◽

Electrospun Nanofibers ◽

Diabetic Rats ◽

Nerve Lesion ◽

Electrospinning Technique ◽

Oxidative Stress Status ◽

Male Wistar Rats ◽

Streptozotocin Induced Diabetes ◽

The Right

In this study, quercetin-loaded zein-based nanofibers were developed using electrospinning technique. The therapeutic effect of these quercetin-loaded nanofibers on neuropathy in streptozotocin- (STZ-) induced diabetes in rats was assessed. Diabetic condition was induced in male Wistar rats by STZ, after which a crush injury of the right sciatic nerve was performed to induce mononeuropathy. Functional recovery was assessed using walking track analysis, measurements of foot withdrawal reflex, nerve conduction velocity, and morphological analysis. The oxidative stress status and the ratio of phosphorylated extracellular recognition kinase (pERK)/extracellular recognition kinase (ERK) expression in the nerve lesion were also assessed in order to elucidate the potential mechanisms involved. Results showed that quercetin-loaded zein-based nanofibers slightly enhanced functional recovery from neuropathy in STZ-diabetic rats. The potential mechanism might partially involve improvements in oxidative stress status and the ratio of pERK/ERK expression in the nerve lesion.

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Isosteviol ameliorates diabetic cardiomyopathy in rats by inhibiting ERK and NF-κB signaling pathways

Journal of Endocrinology ◽

10.1530/joe-17-0681 ◽

2018 ◽

Vol 238 (1) ◽

pp. 47-60 ◽

Cited By ~ 12

Author(s):

Sheng-Gao Tang ◽

Xiao-Yu Liu ◽

Ji-Ming Ye ◽

Ting-Ting Hu ◽

Ying-Ying Yang ◽

...

Keyword(s):

Oxidative Stress ◽

Blood Glucose ◽

Diabetic Cardiomyopathy ◽

Nuclear Factor Κb ◽

Diabetic Rats ◽

Signal Pathways ◽

Mortality And Morbidity ◽

Beneficial Effects ◽

Male Wistar Rats ◽

Glucose Plasma

Diabetes-induced injury of myocardium, defined as diabetic cardiomyopathy (DCM), accounts for significant mortality and morbidity in diabetic population. Alleviation of DCM by a potent drug remains considerable interests in experimental and clinical researches because hypoglycemic drugs cannot effectively control this condition. Here, we explored the beneficial effects of isosteviol sodium (STVNa) on type 1 diabetes-induced DCM and the potential mechanisms involved. Male Wistar rats were induced to diabetes by injection of streptozotocin (STZ). One week later, diabetic rats were randomly grouped to receive STVNa (STZ/STVNa) or its vehicle (STZ). After 11 weeks of treatment or 11 weeks treatment following 4 weeks of removal of the treatment, the cardiac function and structure were evaluated and related mechanisms were investigated. In diabetic rats, oxidative stress, inflammation, blood glucose and plasma advanced glycation end products (AGEs) were significantly increased, whereas superoxide dismutase 2 (SOD-2) expression and activity were decreased. STVNa treatment inhibited cardiac hypertrophy, fibrosis and inflammation, showed similar ratio of heart to body weight and antioxidant capacities almost similar to the normal controls, which can be sustained at least 4 weeks. Moreover, STVNa inhibited diabetes-inducted stimulation of both extracellular signal-regulated kinase (ERK) and nuclear factor κB (NF-κB) signal pathways. However, blood glucose, plasma AGE and insulin levels were not altered by STVNa treatment. These results indicate that STVNa may be developed into a potent therapy for DCM. The mechanism underlying this therapeutic effect involves the suppression of oxidative stress and inflammation by inhibiting ERK and NF-κB without changing blood glucose or AGEs.

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