scholarly journals POTENTIAL OF ENDOPHYTIC FUNGI DERIVING FROM ASIATIC PENNYWORTH TO PRODUCE ANTIOXIDANTS

2021 ◽  
Vol 22 (2) ◽  
pp. 58
Author(s):  
Dwi Ningsih Susilowati ◽  
Nani Radiastuti ◽  
Nurhasni Nurhasni ◽  
Lisca Puji Rustanti ◽  
Ika Roostika Tambunan
Keyword(s):  
Endophytic Fungi ◽  
Color Change ◽  
Antioxidant Activities ◽  
Antioxidant Compounds ◽  
Potato Dextrose Broth ◽  
Fungal Isolates ◽  
Freeze Dried ◽  
Ic50 Value ◽  
Layer Chromatography ◽  
Made In

<p>Asiatic pennyworth is a medicinal plant that contains triterpenoids, saponin, flavonoids, and tannins which possess antioxidants. Endophytic fungi from the plant could produce a similar compound; therefore, antioxidants could be made in the laboratory if the fungi are isolated. This study aimed to evaluate the potential of endophytic fungi isolated from Asiatic pennyworth to produce antioxidants. The study used 34 endophytic fungal isolates from Asiatic pennyworth accessions of Malaysia (17 isolates) and Bengkulu, Indonesia (17 isolates) collected by the Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development. The fungi were propagated in a potato dextrose broth medium, then mycelia mats and filtrates were separated and then freeze-dried. The antioxidant activities were first tested with 1,1-diphenyl-2- picrylhydrazyl (DPPH) using thin layer chromatography (TLC), then UV-Vis spectrophotometry λ517 nm with five variations of concentration. Results showed all 34 fungal isolates have antioxidant activities based on a yellowish-white color change after applying 0.002% DPPH solution of the TLC method and IC50 value of the UV-Vis spectrophotometer. The highest antioxidant activity was shown by <em>Aspergillus austroafricanus </em>MB 1 (IC50 = 12.08 ppm) from Bengkulu accession and <em>A. oryzae </em>MM 13 (IC50 = 10.29 ppm) from Malaysia accession. <em>A. austroafricanus </em>MB 1 produced more antioxidant compounds (seven) than <em>A. oryzae </em>MM 13 (six). The antioxidant compounds produced by both endophytic fungi included in the group of flavonoids, fatty acids, and carboxylic acids. The research implies that <em>A. austroafricanus </em>MB 1 and <em>A. oryzae </em>MM 13 could be further developed as sources of antioxidants.</p>

scholarly journals Orange-Brown Pigment Production from an Endophytic Fungus Aspergillus Sp. N11 and its Potential Pharmaceutical Applications

2022 ◽  
Author(s):  
Madeha Ashfaq ◽  
Qurban Ali ◽  
Abdul Haleem ◽  
Aman Ullah ◽  
Aiman Umar ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Industrial Applications ◽  
Pigment Production ◽  
Brown Pigment ◽  
Zone Of Inhibition ◽  
Potato Dextrose Broth ◽  
Food Industries ◽  
Teucrium Stocksianum ◽  
Aspergillus Sp ◽  
Layer Chromatography

Abstract Endophytic fungi are the main source of natural compounds including pigments having various industrial applications. Present study describes the production of extracellular orange-brown pigment from an endophytic fungal isolate Aspergillus sp. N11from Teucrium stocksianum. The optimum conditions for pigment production from this isolate was investigated and results showed that highest yield was observed in Potato dextrose broth, at pH 5 and 30 ℃ under shaking condition at 150 rpm for 7-10 days. The pigment was extracted in ethyl acetate and purified using column chromatography. Three different pigments were purified (yellow, light brown and orange-brown) and characterized based on Thin layer chromatography and Fourier transform infrared spectroscopy. The antimicrobial activity of purified fragments showed maximum zone of inhibition of 40 mm against S. aureus while for P. aeruginosa maximum zone of 50 mm and maximum antifungal activity of 20 mm against C. albicans. The antioxidant potential of purified pigment obtained from Aspergillus sp. N11 indicates that maximum scavenging activity of 67%. The results showed that purified pigments are astaxanthins belonging to oxygen containing carotenoids. The purified astaxanthins showed antibacterial, antifungal and antioxidant activities indicating its potential to be utilized in pharmaceutical and food industries.

scholarly journals TLC analysis and antioxidant activity of garden egg leaves

2020 ◽  
Vol 12 (1) ◽  
pp. 443-448
Author(s):  
U.A. Umar ◽  
L.G. Hassan ◽  
K.L. Maradun
Keyword(s):  
Antioxidant Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Solanum Melongena ◽  
Solvent System ◽  
Antioxidant Compounds ◽  
Isolation And Characterization ◽  
Layer Chromatography

The Solanum melongena is used traditionally to treat different diseases such as cancer, atherosclerosis, and inflammation. This study is aimed at investigating the thin-layer chromatography analysis and antioxidant activity of n-hexane, acetone and methanol leaves extract of Solanum melongena. Sequential extraction with solvents of increasing polarity was carried out using the n-hexane, ethyl acetate and methanol. The thin-layer chromatography of the extracts carried out with different solvent system. Antioxidant activity was evaluated quantitatively using DPPH (2, 2-diphenyl-2-picrylhydrazyl) for its free radical scavenging ability. The results of thin-layer chromatography revealed some spots with Rf values in the respective extracts, n-hexane (0.31, 0.42, 0.59, 0.65, 0.72, 0.85, 0.92), acetone (0.97, 0.92, 0.88 and 0.59) and methanol (0.72, 0.83, 0.86, 0.94 and 0.96). The extracts exhibit strong antioxidant activities as radical scavengers, indicating that they have strong proton donating abilities. The results from this research show credence to the traditional application of the plant. Further research is recommended on the isolation and characterization of the antioxidant compounds from the plant. Key words: DPPH (2, 2-diphenyl-2-picrylhydrazyl), Thin layer chromatography, Reactive Oxygen Species (ROS)  

scholarly journals ANTIOXIDANT ACTIVITY of THE SECONDARY METABOLITES PRODUCED by ENDOPHYTIC FUNGI ISOLATED from JERUJU (Acanthus ilicifolius L.) PLANT

2019 ◽  
Vol 5 (2) ◽  
Author(s):  
Amanda Rahmaniah Putri ◽  
Salni Salni ◽  
Harry Widjajanti
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Flavonoid Compound ◽  
Antioxidant Compounds ◽  
Strong Activity ◽  
Antioxidant Compound ◽  
Diaporthe Phaseolorum ◽  
Ic50 Value ◽  
Dpph Method

Twelve endophytic fungi were isolated from Jeruju plant. Three of which have the best antioxidant activity, namely Culvularia lunata DJS1, Diaporthe phaseolorum DJS2, and Colletotrichum siamense BJS4. The fungi can produce secondary metabolites that have the potential as antioxidant compounds. This study aims to isolate the antioxidant compound using a chromatography column and test the antioxidant activity using DPPH method. Isolation of secondary metabolites produced 40 eluates. The IC50 value of DJS111 and BJS42 eluates were 10.20 µg/ml and 20.89 µg/ml, which is an antioxidant compound with very strong activity. The IC50 value of DJS24 eluate was 54.45 µg/ml, which is strong antioxidant activity. DJS111 eluate has antioxidant activity that approaches to ascorbic acid as a standart antioxidant compound (7.76µg/ml). From TLC, DJS111 contains flavonoid compound and DJS24 and BJS42 contain terpenoid compound.

scholarly journals Test on the Antioxidant Activities of Methanol Extract of Bidara Leaves (Ziziphus spina-christi L.) using the DPPH Radical Immersion Method

2020 ◽  
Vol 3 (1) ◽  
pp. 44-51
Author(s):  
Dwi Bagus Pambudi ◽  
Nuniek Nizmah Fajriyah ◽  
Vidiah Rizka Shalekhah
Keyword(s):  
Free Radicals ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Chemical Compounds ◽  
Bioactive Compound ◽  
Flavonoid Compound ◽  
Immersion Method ◽  
Compound A ◽  
Ic50 Value ◽  
Layer Chromatography

Bidara (Ziziphus spina-christi L.) is a tropical tree originating from Sudan in which it is commonly known as "Nebeq" in Saudi Arabia. It is of a bioactive compound - a flavonoid compound, which is the potential to be used as an antioxidant. It is capable of inhibiting any cell damages caused by free radicals. This study aimed to measure the activities of the free radicals in methanol extracts of Z. spina-christi leaves. The process of extracting the Z. spina-christi leaves was carried out through the maceration method using methanol as a solvent. The qualitative analysis of chemical compounds with certain eluents using thin-layer chromatography (TLC) was purposely to determine the groups of active compounds in extracts. The measurement of antioxidant activities was carried out using the 1,1-Diphenyl-2-picryl Hidrazyl (DPPH) immersion method in which absorption was measured at a maximum wavelength of 513 nm. The results showed that the methanol extract of Z. spina-christi leaves had a very weak antioxidant activity with the IC50 value of 466.804 �g/ml. The results of the bioautographic profile showed the presence of flavonoid compounds, phenols, saponins, and tannins.

scholarly journals Fungi Endofit dari Tanaman Secang (Caesalpinia sappan L) Sebagai Penghasil Senyawa Antioksidan

2019 ◽  
Vol 5 (1) ◽  
pp. 26-32
Author(s):  
Amirullah Amirullah ◽  
Sartini Sartini ◽  
Firzan Nainu
Keyword(s):  
Elementary Schools ◽  
Endophytic Fungi ◽  
Host Plants ◽  
Antioxidant Activities ◽  
Agar Plate ◽  
Wilcoxon Test ◽  
Antioxidant Compounds ◽  
P Value ◽  
Caesalpinia Sappan ◽  
Fifth Grade Students

Endophytic fungi are microbes that reside symbiotically in the plant tissues with undetected negative impacts to their host plants. These fungi are able to produce elements similar to those produced by their host plants. Secang (Caesalpinia sappan L.) has been known as one of the medicinal plants that contains compounds with antioxidant activity thus promote a hypothesis that its endophytic fungi may yield a similar antioxidant effect. In this study, the isolation of endophytic fungi from Secang as the producer of antioxidant compounds was carried out. At the initial stage, endophytic fungi were isolated from the leaves, the twigs, the stems, and the rootsof Secang using the agar plate method. Pure fungi isolates were then subjected to fermentation process using the PDBgrowth medium supplemented with yeast extractand wereconstantly shaken for 12 days at a speed of 200 rpm to produce secondary metabolites. Fermentatesobtained from all fungi isolates were then extracted using ethyl acetate and further tested for their antioxidant activities. In this study, of 19 isolates that were obtained from four parts of the Secang, five fungi isolates, coded as IFD1, IFD4, IFR5, IFA1, and IFA2 were found to yield antioxidant activities demonstrated by the presence of yellow spots on the chromatogram. elementary schools representatives “not good ranking”, the education did not influence their knowledge with a p value of 0.149. Analysis of the Wilcoxon test in all elementary schools showed a p value p ≤ 0,001, which meant that education had an influence on the knowledge of fifth grade students in elementary schools in Palu City.

Endophytic Fungi - An Untapped Source of Potential Antioxidants

2020 ◽  
Vol 16 (7) ◽  
pp. 944-964
Author(s):  
Rahul C. Mishra ◽  
Mayurika Goel ◽  
Colin J. Barrow ◽  
Sunil K. Deshmukh
Keyword(s):  
Free Radicals ◽  
Endophytic Fungi ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Free Radical Scavengers ◽  
Bioactive Metabolites ◽  
Critical Examination ◽  
Antioxidant Compounds ◽  
Terrestrial Plants ◽  
Micro Organisms

Background: Antioxidants are the substances that interact inside and outside of a biological system against the damaging effects of highly reactive free radicals produced during metabolism. Among various natural alternative sources of bioactive metabolites, endophytic fungi have emerged as a significant reservoir of potent antioxidant compounds. These scantly explored micro-organisms are prolific producers of novel compounds and have the capability to produce metabolites that are exclusively isolated from Plantae. A wide array of compounds like nucleobases, polyketides, terpenoids, flavonoids, coumarins, xanthones, semiquinones, peptides, and phenolic acids have been identified as natural antioxidants produced by these micro-organisms. Methods: A detailed review of the literature published recently was undertaken using bibliographic database like Sci-finder and Google scholar. Questions to be reviewed and criteria for selection as a part of the study were fixed. The key features like information on the structure of isolated metabolites and antioxidant activities were summarised after a critical examination. A skeleton was established which gives insight into the type of novel chemical moieties which can be explored as a future antioxidant (s). Results: The review substantially covers the recently discovered compounds, in the period 2013 – 2018, having potent antioxidant activity, isolated from endophytic fungi colonizing diverse plant types such as terrestrial plants, mangrove plants and marine algae. Among the 96 compounds discussed here, thirtynine are from the first report of their occurrence. The present study reports 96 compounds obtained from 34 endophytic fungi out of which 15 fungi belonging to 13 genera of Ascomycetes produced 44 compounds, 14 fungi belonging to 5 genera of hyphomycetes yielded 33 compounds and 6 fungi belonging to 2 genera of Coelomycetes yielded 19 compounds. : The antioxidant potency of these compounds against different free radicals is briefly described and some details such as host organisms, plant sources, place of collection and the antioxidant properties of these compounds are tabulated in this review. Conclusion: Some of these free radical scavengers have shown wide applications in the food and pharmaceutical industry as potential food preservatives, nutraceuticals, antibacterial, anticancer and antifungal agents. This review aims at highlighting some of the novel compounds isolated recently from endophytic fungi, and their applications as potential antioxidant candidates.

scholarly journals Antibacterial and Antioxidant Activity of Rhodomyrtus Tomentosa and Cinnamomum Zeylanicum Crude Extracts

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Tran Thi Quynh Lan ◽  
Vu Manh Khiem ◽  
Nguyen Van Tin
Keyword(s):  
Antibacterial Activity ◽  
Crude Extract ◽  
Antioxidant Activities ◽  
Inhibition Zone ◽  
Antioxidant Compounds ◽  
Crude Extracts ◽  
Layer Chromatography ◽  
Antibacterial And Antioxidant Activity ◽  
Rhodomyrtus Tomentosa

The aim of this  study was to investigate the extraction method for R. tomentosa and C. zeylanicum leaves and the evaluation of antibacterial and antioxidant activities of crude extracts. The results of the study showed that the active ingredients of crude extracts were  clearly separated by Thin-layer chromatography and the presence of rhodomyrtone in R. tomentosa crude extract and cinnamaldehyde in C. zeylanicum crude extract. R. tomentosa crude  extract was  antibacterial activity against Staphylococcus aureus with 13.1 mm of inhibition zone, but is not effective against Salmonella Typhimurium. C. zeylanicum leaf extract did not show antibacterial activity on both S. aureus and S. Typhimurium. At a dilution of 1/2 of the R. tomentosa crude extract can completely inhibit S. aureus growth. This study also indicated the presence of antioxidant compounds such as flavonoids, tannins, phenols and terpenoids in C. zeylanicum and R. tomentosa crude extracts. The results showed that R. tomentosa and C. zeylanicum crude extracts should be used as a biotherapy alternative to antibiotic therapy. However, further study would be needed to investigate the antibacterial activity of crude extracts in vivo. 

Physiochemical properties and antioxidant potential of Persea Americana seed oil

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Seed Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Acid Value ◽  
Persea Americana ◽  
Antioxidant Potential ◽  
Physiochemical Properties ◽  
Physiochemical Parameters ◽  
Ic50 Value

Oil extracted from Persea Americana seed was assayed for its physiochemical properties and antioxidant potential using various standard methods. The oil content of the seed was found to be &lt; 10%. Brownish-red color oil was liquid at room temperature, with specific gravity of 0.91±0.02 g/mL. Other physiochemical parameters determined were; acid value (4.51±0.08 mgKOH/g), %FFA (2.26±0.08), peroxide value (2.40±0.57 mgO2/Kg), ester value (31.26±0.03 mgKOH/g), saponification value (35.76±0.07 mgKOH/g) and iodine value (23.5±0.07). The results of the antioxidant activities of the seed oil showed that the flavonoid content (80.00±1.41 mgQE/g) was ~10 folds higher than the phenolic content (8.27±0.06 mgGAE/g). The DPPH radical scavenging value was found to be 51.54±0.25% with an IC50 value of 4.68±0.02 mg/mL and reducing power with an average absorbance of 0.85±0.01 and an IC50 value of 0.001±0.02 mg/mL. Gallic acid showed better antioxidant activities than the oil studied. The results obtained in this study showed that Persea Americana seed oil has nutritional, industrial as well as medicinal potentials.

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