Decrease of Spermatogonia, Spermatocytes and Sertoli Cell Counts Male Mice (Mus musculus) Induced by Ethylene Glycol

The experiment was conducted to study the effect of ethylene glycol (EG) on disturbance of spermatogenesis process of the male mice. Sixteen mice at the age of 2 months were randomly into 4 groups of 4 each. The mice of control group were only given drinking water without EG. Group KPI, KP2, and KP3 were given EG 5, 10, and 15 ml/liter drinking water, respectively. On day 31 of post treatment, all mice were killed for their testis. The testis were collected and processed for microscopic examination and haematoxylin-eosin staining. The result showed that EG given orally for 30 days on male mice have negative effect on the process of spermatogenesis as the numbers of spermatogonia, spermatocyte and Sertoli cells decreased significantly (P0.01)

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Curcumin Effectivity on Hepar and Reproductive Organ Recovery Male Mice (Mus musculus L) after Methoxychlor Exposure

Biota ◽

10.20414/jb.v13i1.196 ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Mahriani Mahriani ◽

Susantin Fajariyah ◽

Eva Tyas Utami

Keyword(s):

Mus Musculus ◽

Sertoli Cells ◽

Leydig Cells ◽

Reproductive Organ ◽

Seminiferous Tubules ◽

Control Group ◽

Disruptive Effect ◽

Curcumin Treatment ◽

Cell Counts ◽

Male Reproductive Organ

Methoxychlor (MXC) is an insecticide (DDT derivates) that has the potential for bioaccumulation in mammal and causes a disruptive effect on the hepar and reproductive system. This study was done to find out the benefits of curcumin as a natural ingredient to overcome the negative impact of Methoxychlor (MXC) on hepar and male reproductive organ of Balb’C mice (Mus musculus L). The study was carried out in a Completely Randomized Design (CRD) Posttest Only Control Group Design used four treatments and six replications. The curcumin treatment after administration of MXC was carried out by gavage with curcumin doses: 0.05; 0,1; and 0.2 mg/g body weight, every day for two weeks, respectively. Histological observations of the liver, and testis was performed using the paraffin method and Hematoxylin Eosin stained. The results showed that MXC exposure caused liver disruption by increasing the number of pycnotic necrotic hepatocytes and hydrophic degeneration hepatocytes. On the male reproductive organ, MXC caused testis impairment by reducing the number of Sertoli cells and Leydig cells, spermatogenic cell counts, and the diameter of seminiferous tubules. The administration of curcumin at doses of 0.1 mg/g bw in mice exposed to methoxychlor can reduce the number of hydrophic degeneration hepatocytes and tend to reduce the number of pycnotic hepatocytes; and also increase the number of Sertoli cells, the number of spermatogenic cells, and the diameter of the seminiferous tubules, and tend to reduce the amount of Leydig cells. Curcumin treatment tends to recover hepar dan testis disruption of mice that were exposed by MXC.

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PENGARUH PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum Klotzsch) TERHADAP JUMLAH SEL SERTOLI MENCIT (Mus musculus) YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN

Journal of Basic Medical Veterinary ◽

10.20473/.v8i1.19991 ◽

2020 ◽

Vol 8 (1) ◽

pp. 45

Author(s):

Cyrcilia Relita Berlina ◽

Hana Eliyani ◽

Abdul Sami ◽

Widjiati Widjiati ◽

Sri Mulyati ◽

...

Keyword(s):

Experimental Study ◽

Sertoli Cell ◽

Adult Male ◽

Mus Musculus ◽

Sertoli Cells ◽

Male Mice ◽

Randomized Design ◽

Biophytum Petersianum ◽

Completely Randomized Design ◽

Per Oral

This study was aimed to know the effect of Kebar grass extract on amount of mice Sertoli cell exposed by TCDD. This study was experimental study with completely randomized design. Thirty adult male mice of Mus musculus strain Balb/C, age 11 weeks and weight 25 - 30 ~were used. Mice were divided into five groups, that were: K- was control (aquadest 0) mL); K+ was injected with TCDD at dose of 7 µg/kg BW; Pl was injected with TCCD and administrated with Kebar extract (0,045 mg/ g BW/day); P2 was injected with TCCD and administrated with Kebar extract (0,080 mg/ g BW/day); P3 was injected with TCDD and administrated with Kebar extract (0,135 mg/ g BW/day). Kebar grass extract was administrated in 53 days. Mice were sacrificed and right testis organs were taken. Then, histology preparat with HE staining were made and Sertoli cells were counted. Data were analyzed by One Way ANOV A followed by Duncan test (a = 0,05). The result of this study showed that administration of Kebar grass extract affected amount of mice sertoli cells. K+ (2,92a±0,109) showed significance difference (p<0,05) compared to Pl (5,00b±0,374), P2 (7,64c±0,409) and P3 (9,68ct±0,363). Meanwhile, amount of Sertoli cells of P3 at the highest dose did not show significance difference (p>0,05) with K- (10,16<l±0,829). The conclusion of this study was administration of Kebar grass extract per oral in 53 days could maintain amount of mice Sertoli cell exposed by TCDD.

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Noise Exposure Decreases the Number of Spermatozoa in Male Mice (Mus musculus L.) Even With Grape Seed Extract (Vitis vinifera L.) Administration

WMJ (Warmadewa Medical Journal) ◽

10.22225/wmj.5.2.1785.70-75 ◽

2020 ◽

Vol 5 (2) ◽

pp. 70-75

Author(s):

Ghestiara Siregar

Keyword(s):

Treatment Group ◽

Mus Musculus ◽

Noise Exposure ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

Control Group ◽

Vitis Vinifera L ◽

Not Given ◽

Male Mice

Excessive continuous noises exposure changes the male hormone system which leads to formation of oxidative stress and results in disrupt of semen quality. This condition can be reduced by the use of antioxidants. Grape seed is one of the antioxidants that contains phenol components that have Resveratrol compounds. This research aimed to observe the effect of noise exposure on the number of spermatozoa of male mice given grape seed extract. The method of the research was a post-test only control group design with research subjects of 30 male Mus musculus L. (Swiss Webster) mice divided into 5 groups: group A (treatment control) was not given noise exposure and grape seed extract, group B (negative control) was not given noise exposure and was given grape seed extract, groups C, D, E (treatment group) were given noise exposure with sequential intensities of 65 dB, 85 dB, 105 dB. The treatment was given for 33 days. The results showed that noise exposure with different intensities of 65 dB, 85 dB, 105 dB reduced the spermatozoa count of male Swiss Webster mice even with the administration of grape seed extract. One Way Anova test was used to analyze the data with p-value of 0.001. Conclusion: There were differences in spermatozoa count between the control group and the treatment group. Provision of noise exposure with a value above the threshold limit reduces the number of male Swiss Webster mice spermatozoa given with grape seed extract. Keywords: Number of Spermatozoa, Grape Seed Extract, Noise exposure

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Effect of Aspirin drug in Liver tissue of swiss white mice Mus musculus

Baghdad Science Journal ◽

10.21123/bsj.11.3.1089-1093 ◽

2014 ◽

Vol 11 (3) ◽

pp. 1089-1093

Author(s):

Baghdad Science Journal

Keyword(s):

Acetylsalicylic Acid ◽

Mus Musculus ◽

Liver Tissue ◽

Control Group ◽

Histological Structure ◽

Central Vein ◽

Liver Necrosis ◽

Hepatic Cells ◽

Negative Effect ◽

Radial Arrangement

This study was designed to identify the extent of the effect of drug Acetylsalicylic acid (aspirin) on histological structure of liver in Swiss albino mice Mus musculus.(16) mice were used and divided into two groups. The first groupwas orally treated with )0.5ml(of aspirinat concentration of (50 mcg / kg) and the second group was considered as control group Treated with distiled water lasted for 30 days. Treatment with Aspirin led to the occurrence of histopathological cases included liver necrosis ,cellular infilitration, congestion in central vein and a case of Hemorrhage. The results also showed the absence of radial arrangement of the constituents of hepatic cells from tissue due to these changes with the control group. From the above results, it coulde be concluded that the drug aspirin had a negative effect on liver of Swiss albino

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THE EFFECT OF RED DRAGON FRUIT SKIN EXTRACT (Hylocereus polyrhizus) ON THE NUMBER OF LEYDIG CELLS, DIAMETER OF SEMINIFEROUS TUBULES, AND TESTICULAR WEIGHT OF MALE MICE (Mus musculus) EXPOSURED WITH HOT TEMPERATURES

Ovozoa Journal of Animal Reproduction ◽

10.20473/ovz.v10i1.2021.18-24 ◽

2021 ◽

Vol 10 (1) ◽

pp. 18

Author(s):

Kukuh Prastyaningtyas ◽

Rochmah Kurnijasanti ◽

Rahmi Sugihartuti ◽

Suherni Susilowati ◽

Tri Wahyu Suprayogi ◽

...

Keyword(s):

Mus Musculus ◽

Leydig Cells ◽

Heat Exposure ◽

Seminiferous Tubules ◽

Control Group ◽

Skin Extract ◽

Male Mice ◽

Fruit Peel ◽

Testicular Weight ◽

Hylocereus Polyrhizus

This study aims to determine the effect of red dragon (Hylocereus polyrhizus) fruit peel extract (RDFPE) on the parameters of Leydig cells number, seminiferous tubules diameter, and testicular weight of mice (Mus musculus) exposed to heat (40°C). Twenty adult male mice were divided randomly into five groups. The control group (C) mice only received a placebo. Meanwhile, the treatment groups mice were exposed to heat for 45 minutes daily for 36 days and oral administration of placebo, RDFPE of 250, 500, and 1000mg/kg BW for T0, T1, T2, and T3, respectively. The result showed that heat exposure on mice (T0 group) caused a lower of all of the parameters (p <0.05) than normal mice (control group, C). RDFPE administration at a dose of 250 mg/kg BW (T1 group) and 500 mg/kg BW (T2 group) resulted in a higher value of those parameters (p <0.05) compared to the T0 group. All those parameters of the T2 group (dose of 500 mg/kg BW) were not significantly different (p >0.05) than the control group (normal mice). However, the higher dose of RDFPE (1000 mg/kg BW, T3 group) resulted in the lower values of those parameters (p <0.05) than those of the T2 group. It could be concluded that 500mg/kg BW dose of RDFPE could return Leydig cells number, seminiferous tubules diameter, and testicular weight of mice (Mus musculus) exposed to heat.

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THE EFFECT OF CHRONIC EXPOSURE OF NICOTINE INHALATION TO THE COUNT OF SPERMATOGONIA, SERTOLI CELLS AND LEYDIG CELLS OF YOUNG WHITE RAT WISTAR STRAIN

Indonesian Journal of Urology ◽

10.32421/juri.v26i2.512 ◽

2019 ◽

Vol 26 (2) ◽

Author(s):

Aril Rizaldi ◽

Doddy M Soebadi ◽

Soetojo Soetojo

Keyword(s):

Cell Count ◽

Sertoli Cell ◽

Leydig Cell ◽

Sertoli Cells ◽

Chronic Exposure ◽

Leydig Cells ◽

Control Group ◽

Nicotine Exposure ◽

Control Group Design ◽

Wistar Strain

Objective: To analyze the difference in the number of spermatogonia, leydig cells and sertoli cells in young age of white mice Wistar strain after inhalation of chronic nicotine exposure. Material & Method: Laboratory experimental study with post test only control group design, measurement of spermatogonium, leydig cell, sertoli cell in 5 groups of young male Wistar strain, negative control group and treatment group given nicotine exposure 0.5 mg, 1 mg, 2 mg, and 4 mg/kg body weight/day for 30 days. Results: A significant reduction in spermatogonium was found in the group given nicotine 0.5 mg/kgBW/day (p=0.048), 1 mg/kgBW/day (p=0.002), 2 mg/kgBW/day (p=0.002) and 4 mg/kgBW/day (p=0.000) when compared to the control group. Significant decreases were also seen in the group receiving 4 mg of nicotine exposure compared with 0.5 mg (p=0.018). Significant decrease in sertoli cell count was seen only in the nicotine group of 4 mg/kgBW/day compared with the control group (p=0.047). A significant decrease in leydig cell count was found in the nicotine 2 mg/kgBW/day (p=0.037) and nicotine group 4 mg/kgBW/day (p=0.023) when compared with the control group. Significant decreases were also found in the 4 mg/kgBW/day group compared to the 0.5 mg/kgBW/day group (p=0.004). In this study there were also a decrease in the number of spermatogonia, sertoli cells, and leydig cells in the increased dose of nicotine given although not statistically significant. Conclusion: Chronic exposure of nicotine per inhalation may decrease the number of spermatogonia, sertoli cells, and leydig cells. The higher the dose of nicotine given the greater the decrease in the number of spermatogonium cells, sertoli cells, and leydig cells that occur. This proves that nicotine is one of the causes of infertility in men.

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Efek Analgesik Ekstrak Etanolik Daun Mangrove Api-Api Putih (Avicennia alba) terhadap Mencit (Mus musculus L.) Jantan yang Diinduksi Asam Asetat 0,7%

Hang Tuah Medical journal ◽

10.30649/htmj.v18i1.247 ◽

2020 ◽

Vol 18 (1) ◽

pp. 68

Author(s):

ANDREW THEO WILLIAM CHRIS PURMATA ◽

LESTARI DEWI ◽

CHOESNAN EFFENDI

Keyword(s):

Acetic Acid ◽

Traditional Medicine ◽

Mus Musculus ◽

Analgesic Effect ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Male Mice ◽

Mangrove Species ◽

Avicennia Alba

Background : Avicennia alba or known as mangrove api-api putih is a type of mangrove species which has many benefits and often to be used by the local Indonesians as traditional medicine. The alcaloid and flavonoid substances derived from Avicennia alba leaves could inhibit pain. This study aims to determine the analgesic effect of Avicennia alba leaves extract in male mice (Mus musculus L.) induced by acetic acid 0,7%.Method :25 mice were divided into 5 groups that were given different therapies. The therapy are 10 mL/kg bw of water, 150 mg/kg bw of acetosal, 250 mg/kg bw; 500mg/kg bw; 1000 mg/kg bw of Avicennia alba leaves extract. 0,7% acetic acid with dose of 10 mL/kg bw is given to each subject intraperitoneally 30 minutes after the therapy to induce pain. The pain is manifested as writhes in mice, the writhes then are counted repeatedly each 10 minutes for 30 minutes.Result:: mean of writhes in group treated with Avicennia alba leaves extract in 250 mg/kg bw and 500 mg/kg bw with each value 6,20 and 9,40 are less than mean of writhes in negative control group with value 31,40. %inhibition of Avicennia alba leaves extract with dose of 250 mg/kg bw and 500 mg/kg bw respectively are 80,25% and 70,06%.Conclusion : Avicennia alba leaves extract in 250 mg/kg bw and 500 mg/kg bw dosage showed analgesic effect in male mice (Mus musculus L.) induced with 0,7% acetic acid.Keywords :pain, analgesic, Avicennia alba

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Potensi Ekstrak Rumput Kebar (Biophytum petersianum Klotzsch) Dalam Mempertahankan Jumlah Sel Sertoli Mencit (Mus musculus) Model Diabetes Melitus

Jurnal Penelitian Kesehatan SUARA FORIKES (Journal of Health Research Forikes Voice) ◽

10.33846/sf11311 ◽

2020 ◽

Vol 11 (3) ◽

pp. 277

Author(s):

Etik Yuliarini Widodo ◽

Reny I’tishom ◽

Bambang Purwanto

Keyword(s):

Diabetes Mellitus ◽

Free Radicals ◽

Vitamin E ◽

Mus Musculus ◽

Sertoli Cells ◽

Statistical Tests ◽

Testicular Tissue ◽

Control Group ◽

Biophytum Petersianum ◽

Diabetes Melitus

Background: Hyperglycemia is a common effect of uncontrolled DM. The main impact of DM on male infertility is the result of hyperglycemia which causes an increasing in free radicals which cause oxidative stress in the testes, and then attacks Sertoli cells. Increasing of free radicals will cause disruption and damage to this cell, including apoptosis and atrophy and then caused decreasing in the number of Sertoli cells. Kebar grass contains antioxidants compounds, such as flavonoids and vitamin E. There are synergy effects between vitamin E and flavonoids which reinforce both in working to improve infertility as an antioxidant. Objective: To compared the number of Sertoli cells between groups which receiving extract kebar grass (Biophytum petersianum Klotzsch) and the control group in diabetes mellitus mice (Mus musculus) model. Methods: The research subjects used DM male mice which were divided into 5 groups: 2 control groups (K- and K+) and 3 treatment groups (P1, P2, P3). Day 1 to day 5, all groups were induced by STZ. The 11th day to the 45th day, the control group (K+) is given metformin 2 mg/head /day, the P1, P2 and P3 groups is given kebar grass with dose of 67,5; 135 and 270 mg/kg/day and metformin 2 mg/head/day. The 11th day to the 31th day, the control group (K-) is given CMC-Na 1% suspension. The 46th day is done surgery and sampling testicular tissue. Examination of the number of Sertoli cells is done through examination of the histological picture of testicular tissue that has been given Haematoxylin-Eosin (HE) staining. Results: Statistical tests showed that there was significant differences with a value of p = 0.000 (<0.05) in the number of Sertoli cells. Mean and standard deviation of Sertoli cells in each group were K- = 8.63 ± 0.50; K+ = 9.87 ± 1.52; P1 = 11.40 ± 0.77; P2 = 14.75 ± 1.97 and P3 = 14.97 ± 2.00. Conclusion: Kebar grass extract can maintain the number of Sertoli cells in diabetes mellitus mice models. Keywords: Kebar grass extract; Sertoli cells; diabetes mellitus ABSTRAK Latar belakang: Hiperglikemia adalah efek umum dari DM yang tidak terkontrol. Dampak utama DM terhadap infertilitas pria adalah kondisi hiperglikemik menyebabkan peningkatan radikal bebas dan terjadi stres oksidatif pada testis, kemudian menyerang sel Sertoli. Peningkatan radikal bebas memicu terjadinya gangguan dan kerusakan pada sel-sel tersebut, seperti apoptosis dan atrofi serta terjadi penurunan jumlah sel. Rumput kebar mengandung senyawa yang berperan sebagai antioksidan, seperti flavonoid dan vitamin E. Terdapat efek sinergi antara vitamin E dan flavonoid yang memperkuat kerja keduanya dalam memperbaiki gangguan infertilitas sebagai antioksidan. Tujuan: Membandingkan jumlah sel Sertoli mencit (Mus musculus) model diabetes melitus antara kelompok yang mendapat ekstrak etanol rumput kebar (Biophytum petersianum Klotzsch) dan kelompok kontrol. Bahan dan cara: Subyek penelitian menggunakan mencit jantan DM, dibagi 5 kelompok yaitu 2 kelompok tatist (K- dan K+) dan 3 kelompok perlakuan (P1, P2, P3). Hari ke-1 sampai hari ke-5, semua kelompok diinduksi STZ. Hari ke-11 sampai hari ke-45, kelompok tatist (K-) disonde tatisti CMC-Na 1%, kelompok tatist (K+) disonde metformin 2 mg/ekor/hari, kelompok P1, P2 dan P3 disonde rumput kebar dosis 67,5, 135, 270 mg/kg BB/hari dan metformin 2 mg/ekor/hari, Hari ke – 46 dilakukan pembedahan dan pengambilan sampel jaringan testis kanan dan kiri. Pemeriksaan jumlah sel Sertoli dilakukan melalui pengamatan gambaran histologi jaringan testis yang telah diberikan pewarnaan Haematoxylin-Eosin (HE). Hasil: Uji statistik menunjukkan bahwa terdapat perbedaan bermakna dengan nilai p = 0.000 (< 0,05) pada jumlah sel Sertoli. Mean dan standar deviasi sel Sertoli pada tiap kelompok adalah K- = 8,63 ± 0,50; K+ = 9,87 ± 1,52; P1 = 11,40 ± 0,77; P2 = 14,75 ± 1,97 and P3 = 14,97 ± 2,00. Kesimpulan: Ekstrak rumput kebar dapat mempertahankan jumlah sel Sertoli pada mencit model diabetes melitus. Kata kunci : ekstrak rumput kebar; sel Sertoli; diabetes melitus

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Perubahan Kualitas Sperma Mencit (Mus musculus) Yang Terpapar Asap Rokok Elektrik

BIOTROPIC The Journal of Tropical Biology ◽

10.29080/biotropic.2019.3.2.122-128 ◽

2019 ◽

Vol 3 (2) ◽

pp. 122-128

Author(s):

Andita Ayu Mandasari ◽

Siti Nur Asiyah ◽

Kurnia Lintang

Keyword(s):

Treatment Group ◽

Cigarette Smoke ◽

Mus Musculus ◽

Group Treatment ◽

Time Lag ◽

Cigarette Smoke Exposure ◽

Control Group ◽

Male Mice ◽

Suction Device ◽

Treatment Groups

Smoking is one of the habits that can affect human health. One type of cigarette that is currently the choice is electric cigarettes. The e-cigarette content includes nicotine, propylene glycol, flavorings and glycerin. The purpose of this study was to determine the effect of exposure to e-cigarette smoke on mice strain balb / c with the parameters of the number of spermatozoa. This study used 15 male mice which were 8-10 weeks old and weighed 25-30 grams of male mice which were divided into 3 treatment groups namely control group, treatment group one and treatment group two. In the control group no exposure to cigarette smoke was carried out while in the treatment group one was exposed to cigarette smoke in mice with a dose of 0 mg nicotine and the second treatment group was exposed to e-cigarette smoke at a dose of 18 mg nicotine. Electric cigarette smoke exposure is carried out for 30 days using a suction device from a syringe. The exposure of cigarette smoke is carried out every day with successive doses of 20 times as much as 3 times with a time lag of 10 minutes (5 minutes the chamber is closed and 5 minutes of the chamber is opened). The results showed that the average number of spermatozoa in the control group was 16.816 million / ml. The average number of spermatozoa in treatment one was 10.432 million / ml and the average number of spermatozoa in treatment two was 5.234 million / ml. This shows that exposure to cigarette smoke can reduce the number of spermatozoa from the control group to the first treatment by 42.56% and the second treatment shows a decrease from the first group by 34.65%.

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Oleanolic acid causes reversible contraception in male mice by increasing the permeability of the germinal epithelium

Reproduction Fertility and Development ◽

10.1071/rd18484 ◽

2019 ◽

Vol 31 (10) ◽

pp. 1589 ◽

Cited By ~ 2

Author(s):

David Fisher ◽

Faizel Mosaval ◽

Darla L. Tharp ◽

Doug K. Bowles ◽

Ralf Henkel

Keyword(s):

Oleanolic Acid ◽

Sertoli Cells ◽

Electrical Resistance ◽

Experimental Models ◽

Ethanol Solution ◽

Germinal Epithelium ◽

Control Group ◽

Male Mice ◽

Rt Pcr ◽

Daily Dose

The effects of oleanolic acid (OA) on the fertility of male mice were investigated using both invivo and invitro experimental models. The experimental group (n=12) was treated with a daily dose of 30mgOAkg−1 bodyweight (i.p.), while the control group (n=6) received a daily dose of 10% ethanol solution (1mLkg−1 bodyweight). The effect of OA on the permeability status of TM4 Sertoli monolayers was investigated by measuring the transepithelial electrical resistance (TER), intracellular electrical resistance and semiquantitative RT–PCR. After 45 days, OA-treated males produced no pregnancies but in the control group, all 12 females were impregnated (69 offspring). Male mice, which demonstrated sterility when exposed to OA, recovered their fertility after 30 days (78 offspring). Testicular histological observations of OA-treated mice showed detachment of adjacent Sertoli–Sertoli cells. A control monolayer developed TER of 300–400 Ω.cm2, but OA (50, 100, 200µgL−1) treated monolayers developed TER of approximately 100Ω.cm2. Intracellular electrophysiological and RT–PCR data supported the premise that OA compromised tight junctional permeability. The study demonstrated reversible contraception in male mice by increasing the permeability of the germinal epithelium and further postulates that contraceptive reversibility is brought about by the reconstitution of the paracellular junctions between adjacent Sertoli cells.

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