scholarly journals Potensi Ekstrak Rumput Kebar (Biophytum petersianum Klotzsch) Dalam Mempertahankan Jumlah Sel Sertoli Mencit (Mus musculus) Model Diabetes Melitus

2020 ◽  
Vol 11 (3) ◽  
pp. 277
Author(s):  
Etik Yuliarini Widodo ◽  
Reny I’tishom ◽  
Bambang Purwanto
Keyword(s):  
Diabetes Mellitus ◽  
Free Radicals ◽  
Vitamin E ◽  
Mus Musculus ◽  
Sertoli Cells ◽  
Statistical Tests ◽  
Testicular Tissue ◽  
Control Group ◽  
Biophytum Petersianum ◽  
Diabetes Melitus

Background: Hyperglycemia is a common effect of uncontrolled DM. The main impact of DM on male infertility is the result of hyperglycemia which causes an increasing in free radicals which cause oxidative stress in the testes, and then attacks Sertoli cells. Increasing of free radicals will cause disruption and damage to this cell, including apoptosis and atrophy and then caused decreasing in the number of Sertoli cells. Kebar grass contains antioxidants compounds, such as flavonoids and vitamin E. There are synergy effects between vitamin E and flavonoids which reinforce both in working to improve infertility as an antioxidant. Objective: To compared the number of Sertoli cells between groups which receiving extract kebar grass (Biophytum petersianum Klotzsch) and the control group in diabetes mellitus mice (Mus musculus) model. Methods: The research subjects used DM male mice which were divided into 5 groups: 2 control groups (K- and K+) and 3 treatment groups (P1, P2, P3). Day 1 to day 5, all groups were induced by STZ. The 11th day to the 45th day, the control group (K+) is given metformin 2 mg/head /day, the P1, P2 and P3 groups is given kebar grass with dose of 67,5; 135 and 270 mg/kg/day and metformin 2 mg/head/day. The 11th day to the 31th day, the control group (K-) is given CMC-Na 1% suspension. The 46th day is done surgery and sampling testicular tissue. Examination of the number of Sertoli cells is done through examination of the histological picture of testicular tissue that has been given Haematoxylin-Eosin (HE) staining. Results: Statistical tests showed that there was significant differences with a value of p = 0.000 (<0.05) in the number of Sertoli cells. Mean and standard deviation of Sertoli cells in each group were K- = 8.63 ± 0.50; K+ = 9.87 ± 1.52; P1 = 11.40 ± 0.77; P2 = 14.75 ± 1.97 and P3 = 14.97 ± 2.00. Conclusion: Kebar grass extract can maintain the number of Sertoli cells in diabetes mellitus mice models. Keywords: Kebar grass extract; Sertoli cells; diabetes mellitus ABSTRAK Latar belakang: Hiperglikemia adalah efek umum dari DM yang tidak terkontrol. Dampak utama DM terhadap infertilitas pria adalah kondisi hiperglikemik menyebabkan peningkatan radikal bebas dan terjadi stres oksidatif pada testis, kemudian menyerang sel Sertoli. Peningkatan radikal bebas memicu terjadinya gangguan dan kerusakan pada sel-sel tersebut, seperti apoptosis dan atrofi serta terjadi penurunan jumlah sel. Rumput kebar mengandung senyawa yang berperan sebagai antioksidan, seperti flavonoid dan vitamin E. Terdapat efek sinergi antara vitamin E dan flavonoid yang memperkuat kerja keduanya dalam memperbaiki gangguan infertilitas sebagai antioksidan. Tujuan: Membandingkan jumlah sel Sertoli mencit (Mus musculus) model diabetes melitus antara kelompok yang mendapat ekstrak etanol rumput kebar (Biophytum petersianum Klotzsch) dan kelompok kontrol. Bahan dan cara: Subyek penelitian menggunakan mencit jantan DM, dibagi 5 kelompok yaitu 2 kelompok tatist (K- dan K+) dan 3 kelompok perlakuan (P1, P2, P3). Hari ke-1 sampai hari ke-5, semua kelompok diinduksi STZ. Hari ke-11 sampai hari ke-45, kelompok tatist (K-) disonde tatisti CMC-Na 1%, kelompok tatist (K+) disonde metformin 2 mg/ekor/hari, kelompok P1, P2 dan P3 disonde rumput kebar dosis 67,5, 135, 270 mg/kg BB/hari dan metformin 2 mg/ekor/hari, Hari ke – 46 dilakukan pembedahan dan pengambilan sampel jaringan testis kanan dan kiri. Pemeriksaan jumlah sel Sertoli dilakukan melalui pengamatan gambaran histologi jaringan testis yang telah diberikan pewarnaan Haematoxylin-Eosin (HE). Hasil: Uji statistik menunjukkan bahwa terdapat perbedaan bermakna dengan nilai p = 0.000 (< 0,05) pada jumlah sel Sertoli. Mean dan standar deviasi sel Sertoli pada tiap kelompok adalah K- = 8,63 ± 0,50; K+ = 9,87 ± 1,52; P1 = 11,40 ± 0,77; P2 = 14,75 ± 1,97 and P3 = 14,97 ± 2,00. Kesimpulan: Ekstrak rumput kebar dapat mempertahankan jumlah sel Sertoli pada mencit model diabetes melitus. Kata kunci : ekstrak rumput kebar; sel Sertoli; diabetes melitus

scholarly journals PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum Klotszch) TERHADAP VIABILITAS SPERMATOZOA MENCIT (Mus musculus) DIABETES MELITUS

2021 ◽  
Vol 4 (1) ◽  
pp. 26
Author(s):  
Prima Arundani ◽  
Reny I'tishom ◽  
Bambang Purwanto
Keyword(s):  
Diabetes Mellitus ◽  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Control Group ◽  
Oxygen Species ◽  
Control Group Design ◽  
Group Design ◽  
Biophytum Petersianum ◽  
Post Test ◽  
Diabetes Melitus

<p>Diabetes mellitus yang tidak ditangani dengan baik akan mengakibatkan infertilitas yang disebabkan oleh adanya kerusakan salah satu organ reproduksi yaitu testis. Hiperglikemia atau tingginya kadar gula dalam darah berperan dalam kerusakan sel dengan cara peningkatan <em>reactive oxygen spescies</em> (ROS) sehingga terjadi stres oksidatif jaringan yang mengakibatkan radikal hidroksil. Peningkatan <em>Reactive Oxygen Species </em>(ROS) menyebabkan cedera sel melalui mekanisme peroksidasi lipid dan kerusakan oksidatif protein serta DNA. Proses lipid periksidase pada akhirnya merusak membran spermatozoa dan mitokondria DNA sehingga menyebabkan penurunan kualitas spermatozoa.</p><p><strong>          </strong>Penelitian ini bertujuan mengetahui efek ekstrak rumput kebar terhadap viabilitas spermatozoa mencit jantan (<em>Musmusculus) </em>model diabetes melitus<em>. </em>Penelitian ini merupakan penelitian eksperimental dengan rancangan <em>post test only control group design</em>. Ekstrak rumput kebar menggunakan metode maserasi dengan pelarut etanol 96%. Sebanyak 30 ekor mencit yang dibagi ke dalam 5 kelompok, yaitu kelompok kontrol negatif yaitu mencit diabates mellitus yang di sonde cmc na, kelompok kontrol positif yaitu mencit diabetes mellitus yang di sonde metformin, kelompok I, II, dan III yang di sonde ekstrak rumput kebar dengan dosis 67,5; 130; dan 270 mg/kg BB. Viabilitas spermatozoa dianalisis dengan mengambil sampel spermatozoa dari kauda epididimis.</p><p>Hasil uji Kruskal Wallis menunjukkan hasil yang signifikan p = 0,005 (p ˂ 0,05) pada viabilitas spermatozoa mencit diabetes mellitus dan perbedaan yang bermakna pada kelompok kontrol negatif  mencit dengan diabetes mellitus dengan kelompok perlakuan 3 yang diberi metformin dan ekstrak rumput kebar dosis 270 mg/kg BB. viabilitas spermatozoa pada kelompok kontrol negatif memiliki persentase terendah sedangkan pada kelompok perlakuan 3 memiliki persentase tertinggi. Simpulan penelitian ini adalah ekstrak rumput kebar (<em>Biophytum petersianum </em>Klotzsch) meningkatkan viabilitas spermatozoa mencit (<em>Mus </em>musculus) model diabetes melitus.</p><p> </p>

scholarly journals EFEK JUS BAWANG BOMBAY (ALLIUM CEPA LINN.) TERHADAP MOTILITAS SPERMATOZOA MENCIT YANG DIINDUKSI STREPTOZOTOCIN (STZ)

2017 ◽  
Vol 11 (2) ◽  
pp. 92
Author(s):  
Annisa Hasanah
Keyword(s):  
Allium Cepa ◽  
Mus Musculus ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Diabetes Melitus

Diabetes melitus adalah penyakit metabolik yang ditandai dengan hiperglikemia. Sekitar 90% pria penderita DMmengalami penurunan kualitas spermatozoa. STZ  merupakan bahan toksik yang merusak sel β pankreas. Kandungan quercetin yang tinggi dalam bawang bombay (Allium cepa Linn.) melindungi spermatozoa dari kerusakan. Penelitian ini adalah penelitian eksperimental dengan rancangan posttest only control group design. Besar sampel menggunakan rumus Federer dengan jumlah sampel 32 ekor mencit yang dibagi empat kelompok perlakuan : K0 adalah kelompok kontrol diberi placebo dan jus bawang bombay 1 g/kgBB, K1 adalah kelompok kontrol DM yang diinduksi STZ dosis rendah 50 mg/ kgBB, K2 adalah kelompok induksi STZ dosis rendah dan jus bawang bombay 0,5 g/kgBB, K3 adalah kelompok induksi STZ dosis rendah dan jus bawang bombay 1 g/kgBB. Kualitas spermatozoa yang diperiksa adalah motilitas spermatozoa. Data dianalisis dengan uji Kruskal-Wallis dilanjutkan uji Mann-Whitney. Uji normalitas dengan Saphiro-Wilk didapatkan p<0,05 pada semua parameter (data tidak berdistribusi normal). Hasil uji Kruskal-Wallis didapatkan p<0,05, menunjukkan ada perbedaan signifikan motilitas pada keempat kelompok perlakuan. Uji Mann-Whitney menunjukkan perbedaan signifikan motilitas antar kelompok (p<0,05). Kesimpulan penelitian ini adalah pemberian jus bawang bombay dosis 0,5 g/kgBB dan 1 g/kgBB meningkatkan motilitas spermatozoa mencit yang dijadikan DM dengan induksi STZ.Kata Kunci : Jus bawang bombay (Allium cepa Linn.), motilitas spermatozoa, mencit (Mus musculus), streptozotocin (STZ)

scholarly journals Implementasi Metode Support Group Dalam Meningkatkan Persepsi Pasien Tentang Perawatan Diabetes Melitus di Kota Bandung

2020 ◽  
Vol 7 (1) ◽  
pp. 62-67
Author(s):  
Nandang Jamiat Nugraha ◽  
Rahmat Rahmat
Keyword(s):  
Diabetes Mellitus ◽  
Peripheral Arterial Disease ◽  
Support Group ◽  
Arterial Disease ◽  
Control Group ◽  
Post Test ◽  
Equivalent Control ◽  
Peripheral Arterial ◽  
Diabetes Melitus ◽  
Quasi Experiment

Diabetes Mellitus (DM) merupakan penyakit yang tidak dapat disembuhkan, namun dapat dikendalikan. Prevalensi penderita DM di Indonesia 6.9% dan di Jawa Barat sekitar 29,4% dari jumlah penduduknya. Sebagai mother of desease, penyakit DM memiliki komplikasi yang serius seperti retinopati diabetik, neuropati, amputasi, penyakit jantung, gagal jantung, stroke dan peripheral arterial disease. Kondisi tersebut menunjukkan perlunya keseriusan dalam penanganan penyakit DM. Diperlukan dukungan dari kader (sebagai bagian dari support group) bagi peserta prolanis DM. Tujuan penelitian ini adalah untuk mengetahui implementasi metoda support group dalam meningkatkan persepsi pasien tentang perawatan DM di Kota Bandung. Penelitian ini termasuk jenis penelitian kuantitatif dengan menggunakan rancangan quasi experiment dengan pretest and post test non equivalent control group. Hasil penelitian menunjukkan peningkatan persepsi pasien prolanis sebesar 3,08 dan peningkatannya dinyatakan sangat bermakna (p<0,01).  Edukasi yang dilakukan kader (support group) berpengaruh terhadap peningkatan persepsi pasien DM. Metode support group dengan memberdayakan kader dalam memberikan edukasi sangat bermanfaat dan dapat dijadikan kebijakan di pelayanan kesehatan masyarakat.

scholarly journals Pengaruh Pemberian Antioksidan Vitamin C Dan E Terhadap Konsentrasi Spermatozoa Mencit (Mus Musculus) Yang Dipapar Asap Rokok

2017 ◽  
Vol 5 (1) ◽  
pp. 26
Author(s):  
Ervina Wati Harahap ◽  
Normalina Sandora ◽  
Winarto Winarto
Keyword(s):  
Reactive Oxygen Species ◽  
Free Radical ◽  
Vitamin E ◽  
Vitamin C ◽  
Male Infertility ◽  
Research Design ◽  
Mus Musculus ◽  
Sperm Count ◽  
Control Group ◽  
Oxygen Species

Free radical that are released in cigarette smoke, believed to play an important role in male infertility. The free radicalproduces Reactive Oxygen Species (ROS) that is found increased in male smokers. ROS is one of the free radicalscomponent can be neutralized by vitamin C and E as an antioxidant. This study using those agents to the fifteen minuteshalf piece cigarette to expose mice and count their sperm as the fertilility parameter. The research design used was posttest-only control group with twenty mice were divided into five groups. A group of no smoke exposed was categorizedas a baseline control, the exposed groups were consisted of one group without antioxidant, one group with vitamin Ctreatment, a group of vitamin E treatment and at last the combination of vitamin C and E. All groups were treatedfor fourteen days. The results were analyzed using oneway ANOVA with p<0.05. It is obviously proven that thecombination of vitamin C and vitamin E treatment on the usual dose gave the highest sperm count compared to othergroups.

scholarly journals Pengaruh Pemberian Sakarin terhadap Aktivitas Alanine Aminotransferase Serum Mencit Diabetes Melitus yang Diinduksi Aloksan

2018 ◽  
Vol 7 (1) ◽  
pp. 94
Author(s):  
Mhicya Utami R ◽  
Cimi Ilmiawati ◽  
Elmatris Sy
Keyword(s):  
Mus Musculus ◽  
Alanine Aminotransferase ◽  
Control Group ◽  
Post Test ◽  
Normal Alt ◽  
Diabetes Melitus

Sakarin dijadikan pemanis alternatif pada penderita Diabetes melitus (DM) karena tidak mempengaruhi kadar glukosa darah. Keamanan sakarin mulai diperdebatkan berdasarkan temuan bahwa sakarin mengakibatkan kerusakan hepar yang ditunjukkan dengan peningkatan aktivitas alanine aminotransferase (ALT) serum. Tujuan penelitian ini adalah melihat pengaruh pemberian sakarin terhadap aktivitas ALT serum mencit diabetes melitus yang diinduksi aloksan. Jenis penelitian ini adalah eksperimental dengan rancangan post-test only control group sejak bulan Agustus sampai Oktober 2015 di Laboratorium Farmakologi Fakultas Farmasi Universitas Andalas. Penelitian dilakukan terhadap 20 mencit (Mus musculus) putih jantan diabetes melitus yang diinduksi aloksan yang dibagi menjadi empat kelompok. Kelompok kontrol diberi akuades dan kelompok perlakuan diberi sakarin dengan dosis masing masing 22,75; 45,5; dan 91 mg/kgBB secara oral selama 28 hari. Nilai aktivitas ALT diukur pada akhir penelitian dengan menggunakan alat chemistry analyzer dengan metode spektrofotometri. Hasil penelitian menunjukkan peningkatan nilai aktivitas ALT pada semua kelompok percobaan dibandingkan dengan nilai normal ALT. Analisis statistik mendapatkan aktivitas ALT yang tidak berbeda bermakna antara kelompok percobaan (p = 0,264). Berdasarkan penelitian dapat disimpulkan bahwa pemberian sakarin selama 28 hari tidak memberikan efek yang bermakna pada peningkatan ALT serum mencit diabetes melitus yang diinduksi aloksan.

230 Cryopreservation for Canis lupus conservation: Evaluating protocols to freeze grey wolf testicular tissue

2020 ◽  
Vol 32 (2) ◽  
pp. 243
Author(s):  
C. Andrae ◽  
M. Ferraz ◽  
N. Songsasen ◽  
J. Nagashima
Keyword(s):  
Liquid Nitrogen ◽  
Sertoli Cells ◽  
Canis Lupus ◽  
Testicular Tissue ◽  
Wilcoxon Test ◽  
Cell Populations ◽  
Control Group ◽  
Grey Wolf ◽  
Tissue Samples ◽  
Abnormal Cells

Because of the severe decline in grey wolf (Canis lupus) abundance, preservation of genetic diversity within the species is essential to prevent inbreeding depression. Cryopreservation is a valuable tool in endangered species conservation, because it allows genetic material to be rescued from rare individuals for future use. The ability to preserve gonadal tissue is particularly useful in seasonal breeders, such as grey wolves, when mature gametes are not available during the non-breeding season. Optimization of species-specific cryopreservation protocols is necessary due to the toxicity of cryoprotectants. Here, we aimed to investigate three protocols to cryopreserve grey wolf testicular tissue, as a model for endangered canid species. Testicular tissue samples from four adult grey wolves were obtained and cryopreserved using slow-freezing (SF) and needle vitrification (nVT) techniques. Slow-frozen samples were exposed to 15% dimethylsulfoxide (DMSO) or 7.5% DMSO + 7.5% ethylene glycol (EG) in minimum essential medium (MEM) at room temperature, placed in a Mr. Frosty Freezing Container (Thermo Fisher Scientific) and cooled to −80°C overnight, before storage in liquid nitrogen. Needle-vitrified samples were equilibrated in a 7.5% DMSO + 7.5% EG solution in MEM for 10min at 4°C, then treated with 15% DMSO + 15% EG + 0.5M sucrose for 10min at 4°C before plunging into liquid nitrogen. Each sample was thawed using serial dilutions of sucrose (1, 0.5, and 0.25) into MEM at 37°C for 5min each, fixed in 4% paraformaldehyde, and embedded in paraffin wax. Five-micron-thick cross-sections of tissue samples were stained using haematoxylin and eosin. Tubule areas and quantity of each cell type (Sertoli cells, spermatogonia, spermatocytes, spermatids, and abnormal cells) were recorded and compared with a fresh control group for each wolf. Cell populations and tubule areas were analysed using a nonparametric Wilcoxon test through JMP statistical software (SAS Institute Inc.), and are presented as means±standard deviations. There was no difference in tubule areas between the treatment groups (fresh=5200±2870, nVT=5655±2370, SF DMSO=3607±814, SF DMSO+EG=3905±1450µm2; P&gt;0.05). Although there was an increase in abnormal cells/tubule area (µm2) for nVT, SF DMSO, and SF DMSO+EG compared with the fresh samples (0.0015±0.0014, 0.0014±0.0013, 0.0016±0.0011, and 0.0007±0.0004, respectively; P&gt;0.05), differences were not statistically significant. Moreover, the number of Sertoli cells, spermatogonia, and spermatocytes were reduced in the cryopreserved groups compared with fresh tissues, but the differences were not statistically different (P&gt;0.05). The data suggest that the three tested protocols preserved testicular structure and cell populations of cryopreserved testicular tissue and could be useful in the genetic preservation of grey wolves.

scholarly journals Comparison of Oxidant-Antioxidant Status in Patients with Vitiligo and Healthy Population

2015 ◽  
Vol 12 (2) ◽  
pp. 132-136 ◽  
Author(s):  
S Agrawal ◽  
A Kumar ◽  
TK Dhali ◽  
SK Majhi
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Vitamin E ◽  
Vitamin C ◽  
Control Group ◽  
Healthy Population ◽  
Significant Difference ◽  
Destruction Of Melanocytes ◽  
And Control

Background Vitiligo is a well-recognized pigmentary disorder of the skin and /or mucous membrane characterized by circumscribed ivory or chalky white macules devoid of identifiable melanocytes. The pathogenesis of vitiligo is complex and still not well understood. According to autocytotoxic hypothesis, oxidative stress has been suggested to be the initial pathogenic event in melanocyte degeneration. The role of free radicals and oxidative damage in the pathophysiology of vitiligo has been documented in recent studies.Objective To evaluate the role of oxidative stress in patients with vitiligo and of healthy controls by measuring levels of the oxidant malondialdehyde (MDA) and antioxidants vitamin C and vitamin E in serum and catalase (CAT) in erythrocytes.Method A total of 80 clinically diagnosed cases of vitiligo and 80 control subjects were included in the study to assess the activity of MDA, vitamin C and vitamin E in serum and CAT in erythrocytes of patients and controls by using the spectrophotometric assay.Result There was statistically significant increase in the levels of MDA in patients with vitiligo compared to the control group (p<0.001). No significant difference was found in the levels of vitamin C (p=0.411) and vitamin E (p=0.771) between the patients with vitiligo and control group. The levels of CAT in the vitiligo patients were found to be significantly lower than those of controls (p<0.001).Conclusion Increased oxidative stress and decreased catalase have been observed in vitiligo patients and the data suggesting that the free radicals may be involved in the destruction of melanocytes or dysregulation of melanogenesis.Kathmandu University Medical Journal Vol.12(2) 2014: 132-136

scholarly journals AKTIVITAS FISIK AKUT INTENSITAS TINGGI MEMPERBAIKI KADAR IL-6 OTOT MODEL MENCIT DIABETES MELITUS

2020 ◽  
Vol 8 (3) ◽  
pp. 416
Author(s):  
Septyaningrum Putri Purwoto ◽  
Bambang Purwanto ◽  
Paulus Liben
Keyword(s):  
Physical Activity ◽  
Diabetes Mellitus ◽  
Research Design ◽  
High Intensity ◽  
Design Research ◽  
Control Group ◽  
A Value ◽  
Left And Right ◽  
Random Control ◽  
Diabetes Melitus

Diabetes mellitus is a metabolic disease whose prevalence continues to increase. Diabetes mellitus sufferers are advised to do lifestyle interventions in the form of physical activity. Physical activity provides benefits for diabetics, through the autocrine response of muscles to release IL-6 for muscle repair. The purpose of this study was to determine the appropriate intensity of physical activity against muscle IL-6 in diabetic mice. The subjects in this study were male mice (balb / c) weighing 20-30 grams. This study was divided into 4 groups. The treatment in the form of treadmill activity is carried out once with heating for 5 minutes and core for 30 minutes. This study used a True Experimental research design using a Random Control Group Posttest-Only Design research design. The variable measured in this study was muscle IL-6, namely the calf muscle (gastrocnemious and soleus muscles) of the left and right legs of each subject 24 hours after acute physical activity. Acute physical activity causes the release of interleukin-6 by myofibers. Based on the analysis results showed that there was a significant effect of low, medium, and high intensity acute physical activity on muscle IL-6 levels (p = 0.05). In addition, it also showed that the highest average muscle IL-6 level was the high intensity treatment group with a value of 325.17267 mg / dL. These results prove that high-intensity acute physical activity improves IL-6 levels in the muscle muscle model of diabetes mellitus mice.

scholarly journals Curcumin Effectivity on Hepar and Reproductive Organ Recovery Male Mice (Mus musculus L) after Methoxychlor Exposure

Biota ◽  
2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Mahriani Mahriani ◽  
Susantin Fajariyah ◽  
Eva Tyas Utami
Keyword(s):  
Mus Musculus ◽  
Sertoli Cells ◽  
Leydig Cells ◽  
Reproductive Organ ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Disruptive Effect ◽  
Curcumin Treatment ◽  
Cell Counts ◽  
Male Reproductive Organ

Methoxychlor (MXC) is an insecticide (DDT derivates) that has the potential for bioaccumulation in mammal and causes a disruptive effect on the hepar and reproductive system. This study was done to find out the benefits of curcumin as a natural ingredient to overcome the negative impact of Methoxychlor (MXC) on hepar and male reproductive organ of Balb’C mice (Mus musculus L). The study was carried out in a Completely Randomized Design (CRD) Posttest Only Control Group Design used four treatments and six replications. The curcumin treatment after administration of MXC was carried out by gavage with curcumin doses: 0.05; 0,1; and 0.2 mg/g body weight, every day for two weeks, respectively. Histological observations of the liver, and testis was performed using the paraffin method and Hematoxylin Eosin stained. The results showed that MXC exposure caused liver disruption by increasing the number of pycnotic necrotic hepatocytes and hydrophic degeneration hepatocytes. On the male reproductive organ, MXC caused testis impairment by reducing the number of Sertoli cells and Leydig cells, spermatogenic cell counts, and the diameter of seminiferous tubules. The administration of curcumin at doses of 0.1 mg/g bw in mice exposed to methoxychlor can reduce the number of hydrophic degeneration hepatocytes and tend to reduce the number of pycnotic hepatocytes; and also increase the number of Sertoli cells, the number of spermatogenic cells, and the diameter of the seminiferous tubules, and tend to reduce the amount of Leydig cells. Curcumin treatment tends to recover hepar dan testis disruption of mice that were exposed by MXC.

scholarly journals PENGARUH EKSTRAK BAWANG HITAM TERHADAP PENURUNAN KADAR GLUKOSA DARAH PADA MENCIT

2021 ◽  
Vol 4 (1) ◽  
pp. 49
Author(s):  
Pipit Festi W
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Mus Musculus ◽  
T Test ◽  
Control Group ◽  
Control Group Design ◽  
Significance Level ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Reduce Blood Glucose

 Administration of oral hypoglycemic drugs and insulin is one of the most common pharmacological treatments given to people with diabetes mellitus. This treatment is carried out in the long term so that it causes side effects and is carried out continuously so that it will affect the financial condition of the diabetes mellitus patient. The purpose of this study was to determine the effect of black garlic extract on decreasing blood glucose levels in mice (mus musculus). This study uses the design of True Experimental design (pretest-posttest with control group design). The population is mice in Pusvetma Surabaya. Sampling method uses Non Probability Sampling Purposive Sampling type. Samples taken were 30 mice consisting of 15 intervention groups and 15 control groups. Data was collected using observation sheets, then analyzed using Independent sample t test and Dependent sample t test with a significance level of 5 0.05. The results showed: Using the test Independent sample T test obtained a significance value ρ = 0,000  It can be concluded that there is an effect of black onion extract to reduce blood glucose levels in mice. In the paired t-test test in the treatment group showed ρ = 0,000  that there were differences in blood glucose levels before being given extracts of garlic and after being given intervention. It is expected that black garlic can be used as an alternative medicine to reduce blood glucose levels.Keyword : Ekstrak of black garlic, Blood sugar levels, mus musculus

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