scholarly journals Comparative Transcriptome Analysis of the Roots and Leaves of Bupleurum Chinense DC. Seedlings Under Drought Stress

2021 ◽  
Author(s):  
Ya Fan ◽  
Yannan Geng ◽  
Hailu Cao ◽  
Li Liu ◽  
Quanfang Zhang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Molecular Mechanism ◽  
Drought Resistance ◽  
Fatty Acid Biosynthesis ◽  
Factors Affecting ◽  
Kegg Pathways ◽  
Cellular Processes ◽  
Ssr Analysis ◽  
Before And After ◽  
Leaves And Roots

Abstract Background drought stress is one of the important environmental factors affecting the quality and yield of medicinal materials, and is the main factor restricting the field production of Bupleurum chinense. B. chinense seedlings sensitive to low moisture, but there are few reports on the molecular mechanism of B. chinense seedlings under drought stress. Therefore, the transcriptome of the leaves and roots of B. chinense seedlings before and after drought were analyzed by Illumina sequencing technology and bioinformatics analysis. Results a total of 59.82 GB of clean data was obtained, and the unigenes were compared with Nr, Swissprot, String, GO, KEGG, and Pfam databases. Under drought stress, 3,737 and 6,816 differentially expressed genes (DEGs) were identified in leaves and roots of B. chinense, respectively. The obtained DEGs from leaves and roots were classified into 37, and 36 GO terms and were involved in 222 and 253 KEGG pathways, respectively. SSR analysis were obtained identified 33,728 loci, wherein dinucleotides accounted for the largest proportion. Genes involved in diterpenoid and unsaturated fatty acid biosynthesis were significantly over-expressed in roots under drought stress, suggesting these two cellular processes underpin the adaptation and resistance of B. chinense seedlings to drought stress. Conclusions the results provided a theoretical basis for further identification of the molecular mechanism of drought resistance and breeding of drought resistance varieties of B. chinense.

An Empirical Investigation of Factors Affecting Stock Prices in Vietnam

2014 ◽  
pp. 74-89 ◽  
Author(s):  
Vinh Vo Xuan
Keyword(s):  
Exchange Rates ◽  
Crude Oil ◽  
Foreign Exchange ◽  
Stock Prices ◽  
Global Financial Crisis ◽  
Oil Prices ◽  
Foreign Exchange Rates ◽  
Factors Affecting ◽  
Crude Oil Prices ◽  
Before And After

This paper investigates factors affecting Vietnam’s stock prices including US stock prices, foreign exchange rates, gold prices and crude oil prices. Using the daily data from 2005 to 2012, the results indicate that Vietnam’s stock prices are influenced by crude oil prices. In addition, Vietnam’s stock prices are also affected significantly by US stock prices, and foreign exchange rates over the period before the 2008 Global Financial Crisis. There is evidence that Vietnam’s stock prices are highly correlated with US stock prices, foreign exchange rates and gold prices for the same period. Furthermore, Vietnam’s stock prices were cointegrated with US stock prices both before and after the crisis, and with foreign exchange rates, gold prices and crude oil prices only during and after the crisis.

scholarly journals Arabidopsis MADS-box factor AGL16 negatively regulates drought resistance via stomatal density and stomatal movement

2020 ◽  
Vol 71 (19) ◽  
pp. 6092-6106 ◽  
Author(s):  
Ping-Xia Zhao ◽  
Zi-Qing Miao ◽  
Jing Zhang ◽  
Si-Yan Chen ◽  
Qian-Qian Liu ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Resistance ◽  
Molecular Mechanisms ◽  
Stomatal Density ◽  
Stomatal Closure ◽  
Negative Regulator ◽  
Stomatal Development ◽  
Mads Box ◽  
Mobility Shift ◽  
Aba Metabolism

Abstract Drought is one of the most important environmental factors limiting plant growth and productivity. The molecular mechanisms underlying plant drought resistance are complex and not yet fully understood. Here, we show that the Arabidopsis MADS-box transcription factor AGL16 acts as a negative regulator in drought resistance by regulating stomatal density and movement. Loss-of-AGL16 mutants were more resistant to drought stress and had higher relative water content, which was attributed to lower leaf stomatal density and more sensitive stomatal closure due to higher leaf ABA levels compared with the wild type. AGL16-overexpressing lines displayed the opposite phenotypes. AGL16 is preferentially expressed in guard cells and down-regulated in response to drought stress. The expression of CYP707A3 and AAO3 in ABA metabolism and SDD1 in stomatal development was altered in agl16 and overexpression lines, making them potential targets of AGL16. Using chromatin immunoprecipitation, transient transactivation, yeast one-hybrid, and electrophoretic mobility shift assays, we demonstrated that AGL16 was able to bind the CArG motifs in the promoters of the CYP707A3, AAO3, and SDD1 and regulate their transcription, leading to altered leaf stomatal density and ABA levels. Taking our findings together, AGL16 acts as a negative regulator of drought resistance by modulating leaf stomatal density and ABA accumulation.

Factors Affecting Eyelid Crease Formation Before and After Silicone Frontalis Suspension for Adult-Onset Myogenic Ptosis

2015 ◽  
Vol 31 (3) ◽  
pp. 227-232 ◽  
Author(s):  
Richard C. Allen ◽  
Esther S. Hong ◽  
M. Bridget Zimmerman ◽  
Leslie A. Morrison ◽  
Jeffrey A. Nerad ◽  
...  
Keyword(s):  
Adult Onset ◽  
Factors Affecting ◽  
Before And After ◽  
Frontalis Suspension

Desiccation-tolerance specific gene expression in leaf tissue of the resurrection plant Sporobolus stapfianus

2007 ◽  
Vol 34 (7) ◽  
pp. 589 ◽  
Author(s):  
Tuan Ngoc Le ◽  
Cecilia K. Blomstedt ◽  
Jianbo Kuang ◽  
Jennifer Tenlen ◽  
Donald F. Gaff ◽  
...  
Keyword(s):  
Drought Stress ◽  
Desiccation Tolerance ◽  
Molecular Mechanisms ◽  
Leaf Tissue ◽  
Resurrection Plant ◽  
Specific Gene ◽  
Irreversible Damage ◽  
Cellular Processes ◽  
Sporobolus Stapfianus ◽  
Normal Metabolism

The desiccation tolerant grass Sporobolus stapfianus Gandoger can modulate cellular processes to prevent the imposition of irreversible damage to cellular components by water deficit. The cellular processes conferring this ability are rapidly attenuated by increased water availability. This resurrection plant can quickly restore normal metabolism. Even after loss of more than 95% of its total water content, full rehydration and growth resumption can occur within 24 h. To study the molecular mechanisms of desiccation tolerance in S. stapfianus, a cDNA library constructed from dehydration-stressed leaf tissue, was differentially screened in a manner designed to identify genes with an adaptive role in desiccation tolerance. Further characterisation of four of the genes isolated revealed they are strongly up-regulated by severe dehydration stress and only in desiccation-tolerant tissue, with three of these genes not being expressed at detectable levels in hydrated or dehydrating desiccation-sensitive tissue. The nature of the putative proteins encoded by these genes are suggestive of molecular processes associated with protecting the plant against damage caused by desiccation and include a novel LEA-like protein, and a pore-like protein that may play an important role in peroxisome function during drought stress. A third gene product has similarity to a nuclear-localised protein implicated in chromatin remodelling. In addition, a UDPglucose glucosyltransferase gene has been identified that may play a role in controlling the bioactivity of plant hormones or secondary metabolites during drought stress.

scholarly journals A MicroRNA Signature of Hypoxia

2006 ◽  
Vol 27 (5) ◽  
pp. 1859-1867 ◽  
Author(s):  
Ritu Kulshreshtha ◽  
Manuela Ferracin ◽  
Sylwia E. Wojcik ◽  
Ramiro Garzon ◽  
Hansjuerg Alder ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Hypoxia Inducible Factor ◽  
Tumor Formation ◽  
Low Oxygen ◽  
Functional Link ◽  
Factors Affecting ◽  
Cellular Processes ◽  
Hypoxic Environment ◽  
Tumorigenic Transformation ◽  
First Time

ABSTRACT Recent research has identified critical roles for microRNAs in a large number of cellular processes, including tumorigenic transformation. While significant progress has been made towards understanding the mechanisms of gene regulation by microRNAs, much less is known about factors affecting the expression of these noncoding transcripts. Here, we demonstrate for the first time a functional link between hypoxia, a well-documented tumor microenvironment factor, and microRNA expression. Microarray-based expression profiles revealed that a specific spectrum of microRNAs (including miR-23, -24, -26, -27, -103, -107, -181, -210, and -213) is induced in response to low oxygen, at least some via a hypoxia-inducible-factor-dependent mechanism. Select members of this group (miR-26, -107, and -210) decrease proapoptotic signaling in a hypoxic environment, suggesting an impact of these transcripts on tumor formation. Interestingly, the vast majority of hypoxia-induced microRNAs are also overexpressed in a variety of human tumors.

Response of Interspecific Hybrid Species of Manchurian Ash to MJ and NO Signals and Preliminary Study on the Formation Mechanism of Drought Resistance Advantage

2021 ◽  
Author(s):  
Yang Cao ◽  
fei song ◽  
Xingtang Zhao ◽  
Liming He ◽  
Yaguang Zhan
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Drought Resistance ◽  
Interspecific Hybrid ◽  
Normal Growth ◽  
Control Group ◽  
Physiological Indicators ◽  
Manchurian Ash ◽  
In The Wild ◽  
Set Up

Abstract Background: In this study, sodium nitrate (SNP, a donor of nitric oxide) and methyl jasmonate (MJ) were used as exogenous hormones. The experiment was conducted with the offspring (interspecific hybrid) D110 of ash and ash, and their respective parents (non-interspecific hybrid) D113 and 4-3 as experimental materials. The experiment set up three experimental groups of drought stress, exogenous hormone SNP and MJ, and a control group under normal growth (non-drought stress), to study the physiological indicators and gene expression of manchurian ash. Result: The results showed that under drought stress and exogenous application of hormone SNP or MJ, there were significant differences between hybrids and parents in plant growth, photosynthesis, defense enzyme activity, hormone content and gene expression.Conclusions: This experiment provides a new theoretical support for the existing hormone breeding methods of manchurian ash, which can improve the drought resistance of manchurian ash and increase its survival rate in the wild. Increasing the growth rate and breeding efficiency of manchurian ash brings new ideas.

scholarly journals Tensile Testing with Cyclic Strain Holding to Analyze Dynamic Recrystallization of Pure Lead

2014 ◽  
Vol 2014 ◽  
pp. 1-8
Author(s):  
Mayu Muramatsu ◽  
Motomichi Koyama ◽  
Ikumu Watanabe
Keyword(s):  
Dynamic Recrystallization ◽  
Tensile Testing ◽  
Cyclic Strain ◽  
Tensile Tests ◽  
Pure Lead ◽  
Factors Affecting ◽  
Before And After ◽  
Dynamic Recrystallization Behavior ◽  
The Difference ◽  
Strain Responses

We analyzed the dynamic recrystallization of pure lead by tensile testing with cyclic strain holding at room temperature. The specimens were held at an identical strain and subsequently reloaded, providing the strength before and after the strain holding process. The difference in strength enables factors affecting dynamic recrystallization behavior to be analyzed through mechanical testing. For instance, the effects of strain rate on dynamic recrystallization were analyzed by comparing the results obtained from tensile tests with and without strain holding. This experimental technique demonstrated some parts of contribution of elastic strain, dynamic recovery, dynamic recrystallization, and necking to stress-strain responses.

scholarly journals Drought tolerance molecular responses of two cultivated varieties Jerusalem artichoke (Helianthus tuberosus L.) as revealed by RNA-Seq

2020 ◽  
Author(s):  
Shipeng Yang ◽  
Lihui Wang ◽  
Qiwen Zhong ◽  
Guangnan Zhang ◽  
Haiwang Zhang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Resistance ◽  
Metabolic Pathways ◽  
Jerusalem Artichoke ◽  
Tibet Plateau ◽  
Helianthus Tuberosus ◽  
Rna Seq ◽  
Concentration Gradients ◽  
Light Reaction ◽  
Metabolic Activities

Abstract Background Jerusalem artichoke (Helianthus tuberosus L.) is a highly stress-resistant crop, especially it grows normally in the desertified land of Qinghai-Tibet Plateau in the past two years, and has become a crop with agricultural, industrial and ecological functions. However, there are few studies on drought resistance of Jerusalem artichoke at present, and studies on the mechanisms of stress resistance of Jerusalem artichoke breeding and fructan are seriously lagging behind. In this study, we selected two differentially resistant cultivars for drought stress experiments with different concentration gradients, the aim was finding DEGs and metabolic pathways associated with drought stress. Results Based on an additional analysis of the metabolic pathways under drought stress using MapMan, the most different types of metabolism included secondary metabolism, light reaction metabolism and cell wall. As a whole, QY1 and QY3 both had a large number of up-regulated genes in the flavor pathway. It was suggested that flavonoids could help Jerusalem artichoke to resist drought stress and maintain normal metabolic activities. In addition, the gene analysis of the abscisic acid (ABA) key metabolic pathway showed that QY3 had more genes in NAC and WRKY than QY1, but QY1 had more genes in response to drought stress as a whole. By combining RNA-Seq and WGCNA, a weighted gene co-expression network was constructed and divided into modules. By analyzing specifically the expressed modules, four modules were found to have the highest correlation with drought. Further research on the genes revealed that all 16 genes related to histone, ABA and protein kinase had the highest significance in these pathways. Conclusions These findings represent the first RNA-Seq analysis of drought stress in Jerusalem artichoke, which is of substantial significance to explore the function of drought resistance in Jerusalem artichoke and the excavation of related genes.

scholarly journals USAGE OF YOU TUBE- BASED VIDEOS IN LEARNING DAILY ENGLISH CONVERSATION FOR CHILDREN AT ASRAMA YATIM MIZAN AMANAH, SALIHARA, PASAR MINGGU, SOUTH JAKARTA

Pujangga ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 48
Author(s):  
Evi Jovita Putri ◽  
Gilang Ilham Agustinus ◽  
Intan Kusuma Wardhani
Keyword(s):  
Language Learning ◽  
Test Score ◽  
Qualitative Method ◽  
Influential Factors ◽  
Learning Activities ◽  
Factors Affecting ◽  
Before And After ◽  
Post Test ◽  
Learning English ◽  
Descriptive Qualitative

ABSTRACT This research aims to analyze the effectiveness of YouTube-based videos as a media in learning daily English conversation for children and to identify factors affecting learning daily English conversation for children by using YouTube-based videos as a media. To obtain the aims, this research uses six children whose ages vary from ten to twelve years old lived in Asrama Yatim Mizan Amanah located at Jalan Salihara, Pasar Minggu, South Jakarta as the source of data. Descriptive qualitative method is applied as the research design. In collecting data, the researchers gather the result of learning activities in pre-tests, treatments, and post-tests. Meanwhile, in analyzing the data, the researchers compare the children’s pre-test score with their post-test score to see the development of learning which show their competence before and after being treated. in this case, the research is focused on two topics, Greeting and Introduction in English. The children are introduced some expressions of greeting and introduction in English children by using YouTube-based videos, then some roleplays or conversation containing expressions of greeting and introduction are shown to the children by watching together YouTube-based videos. The last, the children are asked to recognize and practice the expression of greeting and introduction in English. The result shows that the usage of YouTube based video as a media in learning daily English conversation for children is considered effective. Four out of six children are able to memorize new expressions of both greeting and introduction in English, whereas the two other children remains stagnant after learning using YouTube based videos. It is found that there are factors that affect learning English for children by using this media, they are; personality, motivation and attitude, intelligent, learners’ belief, and personality. The most influential factors affecting learning are motivation and attitude. The successful children have these factors stronger rather than the stagnant children. Based on the result, the effectiveness in learning English using YouTube-based videos will be stronger if it is supported by an interactive and conducive learning atmosphere. The learning atmosphere should be considered to achieve a stronger speaking English proficiency. Key words: YouTube based-videos, language learning, daily English conversation ABSTRAK Penelitian ini ditujukan untuk menganalisis keefektifan penggunaan video berbasis YouTube sebagai media dalam belajar percakapan bahasa Inggris sehari-hari pada anak-anak dan untuk mengidentifikasi faktor yang mempengaruhi pembelajaran percakapan bahasa Inggris sehari-hari pada anak-anak yang menggunakan video berbasis YouTube. Untuk mencapai tujuan tersebut, penelitian ini menjadikan enam orang anak berumur sepuluh hingga dua belas tahun yang tinggal di Asrama Yatim Mizan Amanah Jalan Salihara, Pasar Minggu, Jakarta Selatan sebagai sumber data. Penelitian ini menggunakan metode descriptive qualitative. Disini, proses pengumpulan data meliputi pengumpulan hasil pre-tests, treatment, dan post-test. Sementara itu, proses analisis data dilakukan dengan cara membandingkan skor pre-test dengan skor post-test pembelajar untuk melihat perkembangan pembelajaran yang menunjukkan perkembangan anak-anak sebelum dan sesudah pembelajaran. Penelitian difokuskan pada dua topik pembelajaran yaitu sapaan dan perkenalan bahasa Inggris. Dalam hal ini anak-anak diajak mengenal ekspresi sapaan dan perkenalan dalam Bahasa Inggris melalui video yang ada pada YouTube. Kemudian menonton beberapa simulasi dari perilaku menyapa dan berkenalan dalam Bahasa Inggris juga melalui video You-Tube. Pada tahap terakhir, para pembelajar ini diminta untuk berlatih mengingat dan mempraktekkan ungkapan sapaan dan perkenalan dalam Bahasa Inggris Hasil penelitian menunjukkan bahwa penggunaan video berbasis YouTube sebagai media belajar percakapan bahasa Inggris sehari-hari adalah efektif. Empat dari 6 anak mampu mengingat kosakata sapaan dan perkenalan yang baru secara signifikan setelah belajar menggunakan media video berbasis You Tube, meskipun ada 2 anak yang tidak mengalami peningkatan. Ada beberapa faktor yang memengaruhi pembelajaran pada anak-anak tersebut diantaranya kepribadian, motivasi dan sikap, kecerdasan, dan keyakinan. Faktor yang paling berpengaruh pada pembelajaran adalah motivasi dan sikap. Anak-anak yang mengalami peningkatan belajar yang pesat memiliki faktor motivasi dan sikap yang lebih kuat dibanding anak-anak yang tidak mengalami peningkatan. Berdasarkan hasil tersebut, keefektifan dalam belajar percakapan Bahasa Inggris sehari-hari menggunakan video YouTube menjadi lebih besar jika didukung oleh atmosfer belajar yang interaktif dan kondusif. Hal ini mesti diperhatikan guna mencapai kemampuan Bahasa Inggris lisan yang lebih baik. Kata Kunci: Video YouTube, Pembelajaran Bahasa, Percakapan dasar dalam Bahasa Inggris.

Factors affecting post‐relocation adaptation in a case of an entire plant relocation

2002 ◽  
Vol 17 (2) ◽  
pp. 80-94 ◽  
Author(s):  
Moshe Krausz ◽  
Aharon Bizman ◽  
Shaul Fox
Keyword(s):  
Organizational Change ◽  
Work Satisfaction ◽  
Emotional Reactions ◽  
Adaptation Measures ◽  
Factors Affecting ◽  
Family Issue ◽  
Entire Plant ◽  
Before And After ◽  
Plant Relocation

Pre‐relocation measures as well as measures collected subsequent to it were used to predict employees’ post‐relocation adaptation in a case where an entire plant relocated its premises. The distance between the old and the new site did not require home relocation, with the result that the change was deemed less of a “family issue” than an essentially work and organizational change. The sample consisted of 176 employees, for whom matched before and after questionnaires were available. Pre‐relocation measures included demographics, justification of management’s decision to relocate, and positive and negative emotional reactions to the move. In addition, measures of perceived changes were taken after the move. The three post‐relocation adaptation measures were work satisfaction, loyalty to the organization, and relocation satisfaction. Findings are discussed in the light of previous findings, although most of that research dealt with individual as opposed to plant relocation, and of very few studies dealing with post‐relocation variables.

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