PSXV-27 Late-Breaking: Evaluation of antioxidant capacity in receiving beef calves challenged with lipopolysaccharide (LPS)

Abstract The objective of this study was to evaluate antioxidant capacity in plasma of beef calves challenged with LPS. Following an initial feeding period of 40 d, steers (n = 32; 379 kg ± 30.7) were transported to the Livestock Issues Research Unit’s Bovine Immunology Research and Development Complex and challenged intravenously with LPS (0.25 µg/kg BW) on d 41. Blood samples were collected via jugular catheter at -2, 0, 2, 4, 6, 8, 10, 12, 18, 24, 36 and 48 h relative to the LPS challenge at 0 h. Blood samples were processed to isolate plasma for indicators of oxidative stress with a colorimetric assay to determine ferric reducing antioxidant power (FRAP) values via concentration of ferrous iron (µM). Data were analyzed as repeated measures using the GLIMMIX procedure of SAS. Antioxidant values did vary with time (P < 0.001) being greater (P < 0.05) at -2, 0, 2, 36, and 48 h. Antioxidant capacity was reduced at 6 and 8 h (P < 0.05), with the least FRAP value observed at 8 h post-challenge. Antioxidant capacity increased (P < 0.05) again at 10 h, showing similar (P > 0.05) concentrations to those observed at 4 h. By 24 h post-challenge, plasma FRAP values increased (P < 0.05) similar to initial values at -2, 0, and 2 h. It can be inferred that oxidative stress contributes to reduced antioxidant capacity, ultimately interfering with animal growth and productivity. While these values reflect the oxidative stress response to an acute endotoxin challenge, and a subsequent recovery returning to homeostasis within 24 to 48 h, they may also correlate with other physiological and immunological indicators associated with an acute endotoxin challenge.

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Evaluation of Salivary and Serum Total Antioxidant Capacity and Lipid Peroxidation in Postmenopausal Women

International Journal of Dentistry ◽

10.1155/2020/8860467 ◽

2020 ◽

Vol 2020 ◽

pp. 1-5

Author(s):

Fatemeh Zovari ◽

Hadi Parsian ◽

Ali Bijani ◽

Ameneh Moslemnezhad ◽

Atena Shirzad

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Antioxidant Capacity ◽

Postmenopausal Women ◽

Total Antioxidant Capacity ◽

Premenopausal Women ◽

Control Group ◽

Antioxidant Power ◽

Whole Saliva ◽

Total Antioxidant

Objective. In menopause, reduction of estrogen hormone affects oxidative stress process in serum. Oxidative stress in saliva plays a significant role in the pathogenesis of oral diseases. The aim of this study was to investigate the total antioxidant capacity and lipid peroxidation in the serum and saliva of premenopausal and postmenopausal women. Methods. In this case control study, 50 postmenopausal women (case group) and 48 premenopausal women (control group) were selected. The unstimulated whole saliva and serum of the postmenopausal and premenopausal women were collected. The total antioxidant capacity (TAC) of the saliva and serum was measured by ferric reducing antioxidant power (FRAP). Also, malondialdehyde (MDA) was measured by thiobarbituric acid reactive substance (TBARS) method for serum and saliva. Then, the obtained data were analyzed by SPSS 17, whereby Mann–Whitney test and Spearman’s correlation test were used. P < 0.05 was considered statistically significant. Results. The postmenopausal group had significantly lower mean serum TAC and higher mean serum MDA than the control group ( P < 0 < 001 and P < 0.01 , respectively). The mean salivary TAC and MDA, however, did not differ significantly between the case and control group ( P = 0.64 and P = 0.08 , respectively). Conclusion. In postmenopausal women, with elevation of serum MDA and reduction of serum TAC, the extent of serum oxidative stress grows, but MDA and TAC levels of saliva do not change.

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Effect of Mixed Flavonoids, n-3 Fatty Acids, and Vitamin C on Oxidative Stress and Antioxidant Capacity Before and After Intense Cycling

International Journal of Sport Nutrition and Exercise Metabolism ◽

10.1123/ijsnem.21.4.328 ◽

2011 ◽

Vol 21 (4) ◽

pp. 328-337 ◽

Cited By ~ 13

Author(s):

Steven R. McAnulty ◽

David C. Nieman ◽

Lisa S. McAnulty ◽

Worley S. Lynch ◽

Fuxia Jin ◽

...

Keyword(s):

Oxidative Stress ◽

Fatty Acids ◽

Vitamin C ◽

Antioxidant Capacity ◽

Repeated Measures ◽

Statistical Design ◽

Potential Health ◽

Plasma Antioxidant Capacity ◽

Plasma Antioxidant ◽

Post Hoc

Consumption of plant flavonoids, antioxidants, and n-3 fatty acids is proposed to have many potential health benefits derived primarily through antioxidant and anti-inflammatory activities. This study examined the effects of 1,000 mg quercetin + 1,000 mg vitamin C (QC); 1,000 mg quercetin, 1,000 mg vitamin C, 400 mg isoquercetin, 30 mg epigallocatechin gallate, and 400 mg n-3 fatty acids (QFO); or placebo (P), taken each day for 2 wk before and during 3 d of cycling at 57% Wmax for 3 hr, on plasma antioxidant capacity (ferricreducing ability of plasma [FRAP], oxygen-radical absorbance capacity [ORAC]), plasma oxidative stress (F2-isoprostanes), and plasma quercetin and vitamin C levels. Thirty-nine athletes were recruited and randomized to QC, QFO, or P. Blood was collected at baseline, after 2 wk supplementation, immediately postexercise, and 14 hr postexercise. Statistical design used a 3 (groups) × 4 (times) repeated-measures ANOVA with post hoc analyses. Plasma quercetin was significantly elevated in QC and QFO compared with P. Plasma F2-isoprostanes, FRAP, and vitamin C were significantly elevated and ORAC significantly decreased immediately postexercise, but no difference was noted in the overall pattern of change. Post hoc analyses revealed that the QC and QFO groups did not exhibit a significant increase in F2-isoprostanes from baseline to immediately postexercise compared with P. This study indicates that combining flavonoids and antioxidants with n-3 fatty acids is effective in reducing the immediate postexercise increase in F2-isoprostanes. Moreover, this effect occurs independently of changes in plasma antioxidant capacity.

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Salivary antioxidant status and oral health in children and adolescents

New Medicine ◽

10.25121/newmed.2019.23.4.145 ◽

2019 ◽

Vol 23 (4) ◽

Author(s):

Paula Piekoszewska-Ziętek ◽

Emilia Raćkowska ◽

Natalia Korytowska ◽

Dorota Olczak-Kowalczyk

Keyword(s):

Oxidative Stress ◽

Oral Health ◽

Antioxidant Capacity ◽

Children And Adolescents ◽

Oral Hygiene ◽

Protective Factor ◽

Salivary Flow ◽

White Spot ◽

White Spot Lesions ◽

Antioxidant Power

Introduction. The pathogenesis of oral diseases may be associated with oxidative stress. Salivary antioxidant system constitutes one of the key salivary defence mechanisms against pathogens and a protective factor for oral cavity. Aim. To investigate the relationship between oral health (hygiene level, gingival and dental health), age and gender and antioxidant capacity parameters in children and adolescents with permanent dentition. Material and methods. A total of 87 patients were examined. DMFT/DMFS and white spot lesions (WSL), oral hygiene level and gingival health were assessed. Salivary samples were collected from all participants. Unstimulated salivary flow was calculated and salivary samples were assayed for total antioxidant capacity (TAC) and ferric reducing antioxidant power (FRAP). Results. Antioxidant capacity parameters were lower in patients with caries, active caries, white spot lesions, poor oral hygiene and gingivitis, but the differences were not statistically significant. Oxidative stress parameters were significantly higher in low unstimulated salivary flow. Spearman’s rank correlation analysis revealed no relationship between TAC or FRAP values and patients’ gender, but there was a positive correlation between TAC/FRAP and patients’ age. Conclusions. Salivary antioxidant capacity parameters differ in certain oral conditions. There is a correlation between salivary antioxidant capacity parameters and patients’ age.

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Antioxidant capacity of lipid- and water-soluble antioxidants in dogs with subclinical myxomatous mitral valve degeneration anaesthetised with propofol or sevoflurane

10.21203/rs.3.rs-18067/v2 ◽

2020 ◽

Author(s):

Katerina Tomsič ◽

Alenka Nemec Svete ◽

Ana Nemec ◽

Aleksandra Domanjko Petrič ◽

Tatjana Pirman ◽

...

Keyword(s):

Oxidative Stress ◽

Mitral Valve ◽

Antioxidant Capacity ◽

Early Stage ◽

Water Soluble ◽

Oxygen Species ◽

Dental Procedure ◽

Blood Samples ◽

Sampling Times

Abstract Background: Antioxidants located in both the hydrophilic and lipophilic compartments of plasma act as a defence system against reactive oxygen species (ROS). . Excessive production of ROS during anaesthesia affects the antioxidant capacity of plasma and may result in oxidative stress. The aim of this study was to evaluate the antioxidant capacity of lipid- (ACL) and water-soluble (ACW) antioxidants in client-owned dogs diagnosed with periodontal disease and early-stage myxomatous mitral valve degeneration (MMVD) and anaesthetised for a dental procedure with propofol and sevoflurane or with propofol only. Results: Dogs with MMVD were anaesthetised with propofol and sevoflurane (MMVD/PS, n = 8) or with propofol only (MMVD/P, n = 10). Dogs with no evidence of MMVD (PS, n = 12) were anaesthetised with propofol and sevoflurane. Blood samples for determination of ACL and ACW were collected before and 5 minutes, 60 minutes and 6 hours after induction to anaesthesia. In dogs with MMVD and anaesthetised with propofol and sevoflurane, ACL was significantly higher at all sampling times when compared to control dogs. Compared to basal values, only anaesthesia maintained with propofol significantly increased ACL at 60 minutes in dogs with MMVD. In dogs with MMVD and anaesthetised with propofol, ACW increased after induction to anaesthesia and remained elevated up to 6 hours after anaesthesia. Compared to basal values, anaesthesia maintained with sevoflurane significantly increased ACW only at 60 minutes in both dogs with and without MMVD.The only difference between propofol and propofol/sevoflurane anaesthesia in dogs with MMVD was significantly higher ACW at 60 minutes after induction to anaesthesia in the propofol group. Conclusions: Regarding antioxidant capacity, propofol could be a better choice than sevoflurane for anaesthesia of dogs with early-stage MMVD, although further studies are necessary to clarify the advantage of this antioxidant capacity.

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Effect of pharmacological lowering of plasma urate on exercise-induced oxidative stress

Applied Physiology Nutrition and Metabolism ◽

10.1139/h07-131 ◽

2007 ◽

Vol 32 (6) ◽

pp. 1148-1155 ◽

Cited By ~ 9

Author(s):

S. R. McAnulty ◽

P. A. Hosick ◽

L. S. McAnulty ◽

J. C. Quindry ◽

L. Still ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Capacity ◽

Repeated Measures ◽

Lipid Hydroperoxides ◽

Double Blind ◽

Reducing Ability ◽

Plasma Antioxidant Capacity ◽

Plasma Urate ◽

Exercise Induced ◽

The Impact

Urate is a metabolic end product of purine metabolism that contributes about 66% of the antioxidant capacity of plasma. The objective of this study was to evaluate the importance of plasma urate as an antioxidant using pharmacological lowering and examining the impact on plasma antioxidant capacity and oxidative stress after intense exercise. Fifteen subjects ran for 45 min at ~80% VO2 max under the influence of probenecid (1 g/d) (PRO) or placebo (PLA) in a double-blind, crossover design. Blood samples obtained at baseline, pre-exercise, and immediately post-exercise were analyzed for F2-isoprostanes, lipid hydroperoxides (LHs), ferric-reducing ability of plasma (FRAP), urate, ascorbate (AA), and nitrite. A 2 (group) × 2 (time) repeated-measures analysis of variance (ANOVA), one-way ANOVA, Tukey–Kramer multiple comparison tests, and Student’s t tests were used for statistical analysis. PRO exhibited lowered urate and FRAP compared with baseline (p ≤ 0.05), and group effects existed for the exercise trials (p = 0.023 and p ≤ 0.001, respectively) versus PLA. F2-isoprostanes, nitrite, and AA were increased after exercise (p = 0.004, p = 0.001, and p = 0.003, respectively), but the pattern of change was not different between treatments. This study indicates that plasma markers of exercise-induced oxidative stress were not affected by below-normal physiological concentrations of urate and a diminished antioxidant capacity within the plasma compartment.

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Dietary Antioxidant Capacity Promotes a Protective Effect against Exacerbated Oxidative Stress in Women Undergoing Adjuvant Treatment for Breast Cancer in a Prospective Study

Nutrients ◽

10.3390/nu13124324 ◽

2021 ◽

Vol 13 (12) ◽

pp. 4324

Author(s):

Luiza Kuhnen Reitz ◽

Jaqueline Schroeder ◽

Giana Zarbato Longo ◽

Brunna Cristina Bremer Boaventura ◽

Patrícia Faria Di Pietro

Keyword(s):

Breast Cancer ◽

Oxidative Stress ◽

Prospective Study ◽

Antioxidant Capacity ◽

Adjuvant Treatment ◽

Lipid Hydroperoxides ◽

Antioxidant Power ◽

Dietary Antioxidant ◽

Increased Risk ◽

Dietary Antioxidant Capacity

Breast cancer (Bca) is the most common type of cancer among women worldwide, and oxidative stress caused by adjuvant treatment may be decreased by antioxidant intake. The aim of this study is to investigate the associations between Dietary antioxidant Capacity (DaC) and oxidation and antioxidant biomarkers in women undergoing adjuvant treatment (AT) for Bca. This prospective study had a sample of 70 women (52.2 ± 10.7 y). DaC (mmol/g) was calculated using nutritional data obtained from a Food Frequency Questionnaire, and blood was collected to measure the oxidation and antioxidant biomarkers at baseline (T0), and after AT (T1). Carbonylated protein levels were inversely associated with DaC at T1 (p = 0.004); women showed an increased risk of having increment on lipid hydroperoxides and thiobarbituric acid reactive substances (TBARS), and decrement on ferric reducing antioxidant power (FRAP) and reduced glutathione after AT, in response to lowered DaC (p < 0.05). Carbonylated proteins, TBARS and FRAP levels remained stable between the periods for women at the 3rd DaC tertile at T1, differentiating them from those at the 1st tertile, who showed negative changes in these biomarkers (p < 0.04). DaC may be beneficial for women undergoing AT for Bca, since it promoted a reduction in oxidative stress.

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In vitro, antioxidant activities of aqueous and methanol roselle (Hibiscus sabdariffa) calyces extracts from two localities in Cameroon

The North African Journal of Food and Nutrition Research - July-December 2021 ◽

10.51745/najfnr.4.08.292-297 ◽

2020 ◽

Vol 4 (08) ◽

pp. 292-297

Author(s):

Ghislain Maffo Tazoho ◽

Esther Etengeneng Agbor ◽

Inocent Gouado

Keyword(s):

Oxidative Stress ◽

Free Radical ◽

Antioxidant Capacity ◽

Aqueous Extract ◽

Methanol Extract ◽

Total Phenolic ◽

Hibiscus Sabdariffa ◽

Antioxidant Power ◽

Methanol Extracts ◽

Significant Difference

Background: Fighting against malnutrition and diseases such as oxidative stress diseases via a food-based approach could be achieved through identification, valorization, and promotion of local foods rich in macro- and micronutrients and phytochemical components. Aim: This study aimed to investigate the effect of agro-ecological conditions on the antioxidant capacity of the Hibiscus sabdariffa aqueous and methanol calyces extracts. Material and methods: The total phenolic content, the free radical DPPH• (1,1-diphenyl-2-picrylhydrazyl), and ferric reducing antioxidant power (FRAP) activities were evaluated in aqueous and methanol Hibiscus sabdariffa calyces extract samples from two localities (Dschang [western highland zone] and Ngaoundéré [high Guinean savanna zone]) in Cameroon. Results: The results obtained showed that these extracts contain an important amount of total phenolic compounds with no significant difference (P>0.05) between aqueous and methanol extracts and also between the origins of calyces. The extracts showed their ability to reduce Fe3+ to Fe2+ as well as their ability to reduce the free radical, DPPH•. Concerning the FRAP results, at the concentration of 12.5µg/mL, the absorbance of AEN (Aqueous Extract from Ngaoundéré locality) recorded the value (0.33±0.05) meanwhile at a far higher concentration of 200 µg/mL, the absorbance of MEN (Methanol Extract from Ngaoundéré locality) was the highest (1.39±0.06). The CI50 of methanol extracts was significantly low (P<0.05) compared to that of aqueous extracts. The value ranges were 11.31±0.15, 14.69±0.84, 18.07±0.63, 20.50±0.54, and 21.50±0.54 µg/mL for ascorbic acid, MEN (Methanol Extract from Ngaoundéré locality), MED (Methanol Extract from Dschang locality), AEN (Aqueous Extract from Ngaoundéré locality), and AED (Aqueous Extract from Dschang locality) respectively. Conclusion: These findings show that the antioxidant activity of methanol extract is higher than that of aqueous extract. Hibiscus calyces from the two localities could be used as a natural antioxidant in the treatment of oxidative stress diseases. Keywords: Roselle calyces, aqueous and methanol extracts, antioxidant capacity.

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Patients Undergoing Surgery for Hip Fractures Suffer from Severe Oxidative Stress as Compared to Patients with Hip Osteoarthritis Undergoing Total Hip Arthroplasty

Oxidative Medicine and Cellular Longevity ◽

10.1155/2021/5542634 ◽

2021 ◽

Vol 2021 ◽

pp. 1-10

Author(s):

Theofilos Karachalios ◽

Dionyssios Paridis ◽

Fotios Tekos ◽

Zoi Skaperda ◽

Aristidis S. Veskoukis ◽

...

Keyword(s):

Oxidative Stress ◽

Total Hip Arthroplasty ◽

Bone Turnover ◽

Antioxidant Capacity ◽

Hip Fractures ◽

Hip Arthroplasty ◽

Hip Osteoarthritis ◽

First Line ◽

Blood Samples ◽

Oxidative Defense

Hip fractures are associated with the highest degree of morbidity and mortality of all fractures in elderly patients and pose a major risk for subsequent fractures. Patients with hip fractures also present accelerated bone turnover despite early stable fracture fixation and early mobilization. We aimed to evaluate oxidative stress in two groups of patients (25 patients each, matched for age, side, and BMI) who underwent internal fixation of hip fractures and total hip arthroplasty for hip osteoarthritis. Blood samples were taken from all patients during admission, the day of surgery, the 4th postoperative day, and the 15th postoperative day. Reduced (GSH) and oxidized (GSSG) glutathione, GSH/GSSG, catalase (CAT), thiobarbituric acid reactive substances (TBARS), protein carbonyls (PC), and total antioxidant capacity (TAC) as a widely used battery of redox biomarkers were recorded from blood samples. Patients with hip fractures who undergo fixation surgery, compared to those with hip osteoarthritis, suffer significant oxidative stress with an active but insufficient first line of oxidative defense, an intensive first line reaction, a very active second line of oxidative defense, and a low plasma antioxidant capacity. Surgery worsened already present lipid- and protein-related tissue damage. The severe oxidative stress observed may explain high morbidity and mortality rates and high bone turnover status, as well as the high incidence of refractures. Furthermore, the question of whether antioxidant therapy measures should be introduced in the management of hip fracture patients is raised.

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Oxidative Stress Modulation by Cameroonian Spice Extracts in HepG2 Cells: Involvement of Nrf2 and Improvement of Glucose Uptake

Metabolites ◽

10.3390/metabo10050182 ◽

2020 ◽

Vol 10 (5) ◽

pp. 182 ◽

Cited By ~ 1

Author(s):

Achille Parfait Atchan Nwakiban ◽

Stefania Cicolari ◽

Stefano Piazza ◽

Fabrizio Gelmini ◽

Enrico Sangiovanni ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Capacity ◽

Glucose Uptake ◽

Gallic Acid ◽

Hepg2 Cells ◽

Biological Activities ◽

Array Detector ◽

Ros Scavenging ◽

Antioxidant Power ◽

Tetrapleura Tetraptera

Oxidative stress plays a relevant role in the progression of chronic conditions, including cardiometabolic diseases. Several Cameroonian plants, including spices, are traditionally used as herbal medicines for the treatment of diseases where oxidative stress contributes to insulin resistance, like type 2 diabetes mellitus. This study evaluated the antioxidant capacity and the effects on oxidative-stress-induced impairment of glucose uptake of 11 Cameroonian spice extracts. H2O2-induced reactive oxygen species (ROS) production by human HepG2 cells was significantly reduced by 8/11 extracts. The most effective extracts, Xylopia parviflora, Echinops giganteus, and Dichrostachys glomerata, showed a concentration-dependent ROS-scavenging activity, which involved Nrf2 translocation into the nucleus. Xylopia parviflora, Tetrapleura tetraptera, Dichrostachys glomerata, Aframomum melegueta, and Aframomum citratum extracts showed the highest antioxidant capacity, according to oxygen radical absorbance capacity (ORAC) (2.52–88 μM Trolox Eq/g of extract), ferric-reducing antioxidant power (FRAP) (40.23–233.84 mg gallic acid Eq/g of extract), and total phenol (8.96–32.96% mg gallic acid Eq/g of extract) assays. In HepG2 cells, glucose uptake was stimulated by 4/11 extracts, similarly to insulin and metformin. H2O2-induced oxidative stress reduced glucose uptake, which was rescued by pretreatment with Xylopia aethiopica, Xylopia parviflora, Scorodophloeus zenkeri, Monodora myristica, and Dichrostachys glomerata extracts. The ROS-scavenging ability of the spice extracts may reside in some secondary metabolites observed by phytochemical profiling (reverse-phase high-performance liquid chromatography coupled to a diode array detector (HPLC-UV-DAD)). Further studies are needed to better clarify their biological activities and potential use to control oxidative stress and promote insulin sensitivity.

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Effects of dietary supplementation with exogenous catalase on growth performance, oxidative stress, and hepatic apoptosis in weaned piglets challenged with lipopolysaccharide

Journal of Animal Science ◽

10.1093/jas/skaa067 ◽

2020 ◽

Vol 98 (3) ◽

Cited By ~ 1

Author(s):

Yang Li ◽

Xilun Zhao ◽

Xuemei Jiang ◽

Ling Chen ◽

Liang Hong ◽

...

Keyword(s):

Oxidative Stress ◽

Growth Performance ◽

Mrna Expression ◽

Rectal Temperature ◽

Caspase 3 ◽

Dietary Supplementation ◽

Basal Diet ◽

Weaned Piglets ◽

Post Challenge ◽

Blood Samples

Abstract Two experiments were conducted to investigate the effects of exogenous catalase (CAT) in the diet of weaned piglets on growth performance, oxidative capacity, and hepatic apoptosis after challenge with lipopolysaccharide (LPS). In experiment 1, 72 weaned piglets [Duroc × Landrace × Yorkshire, 6.90 ± 0.01 kg body weight (BW), 21 d of age] were randomly assigned to be fed either a basal diet (CON group) or a basal diet supplemented with 2,000 mg/kg CAT (CAT group; dietary CAT activity, 120 U/kg) for 35 d. Blood samples were collected on day 21 and day 35. At the end of this experiment, 12 pigs were selected from each of the CON and CAT groups, and six pigs were injected with LPS (50 μg/kg BW), while the remaining six pigs were injected with an equal amount of sterile saline, resulting in a 2 × 2 factorial arrangement of treatments (experiment 2). Blood samples and rectal temperature data were collected 0 and 4 h after challenge, and liver samples were obtained after evisceration. The gain-to-feed ratio was higher (P < 0.05) in piglets in the CAT group than in those in the CON group from day 1 to 35. Catalase and total superoxide dismutase (T-SOD) activities were higher (P < 0.05), whereas malondialdehyde (MDA) concentrations were lower (P < 0.05), in piglets in the CAT group than in those in the CON group at day 35. During challenge, rectal temperature and liver MDA and H2O2 concentrations increased significantly (P < 0.05), whereas plasma CAT and glutathione peroxidase (GSH-Px) activities and liver CAT activity decreased markedly (P < 0.05), in LPS-challenged piglets 4 h post-challenge. Increased CAT activity and decreased MDA concentration were observed in the plasma and liver of piglets in the CAT group 4 h post-challenge (P < 0.05). Dietary CAT supplementation markedly suppressed the LPS-induced decrease in plasma GSH-Px activity and liver CAT activity to levels observed in the CON group (P < 0.05) as well as significantly decreasing the concentration and mRNA expression of caspase-3 and caspase-9 (P < 0.05). LPS-induced liver injury was also attenuated by dietary CAT supplementation, as demonstrated by a decrease in liver caspase-3 mRNA expression (P < 0.05). Overall, dietary supplementation with 2,000 mg/kg exogenous CAT (dietary CAT activity, 120 U/kg) improves growth performance and has a beneficial effect on antioxidant capacity in weaned piglets; alleviates oxidative stress and reduces liver damage by suppressing hepatic apoptosis in LPS-challenged piglets.

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