scholarly journals Healing Features of Experimental Injuries of Soft Tissues That Contain Foreign Bodies In The Form of Fragments Of Military Personnel Uniforms

2020 ◽  
Author(s):  
Sergey Pavlov ◽  
Olga Litvinova ◽  
Rostislav Mikhaylusov ◽  
Vladimir Negoduyko ◽  
Marina Kumetchko ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Soft Tissue ◽  
Foreign Bodies ◽  
Soft Tissues ◽  
Normal Values ◽  
Structural Features ◽  
Laboratory Animals ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inflammatory Reactions

Abstract Background The healing of combat wounds can be complicated by the presence of foreign bodies (FBs) in the form of fragments of military uniforms in the wound canal. During the experiment, the structural features of the regeneration of soft tissue injuries complicated by FBs in the form of fragments of two types of military uniforms were studied. Changes in the content of the basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) during wound healing were studied.Methods By randomization, 60 rats were divided into 4 groups: intact (Int), control (Con) and two experimental (EG1, EG2). A layered incision of the thigh soft tissues was performed on the animals. In Con, wounds were sutured without implantation of FBs. A camouflage uniform consisting of 100% cotton was used as FBs for EG1, a uniform consisting of 65% cotton and 35% polyester was used for EG2. The size of the implanted fragments was 0.5 × 0.5 cm. The removal of laboratory animals from the experiment was carried out in 6 of each group on the 15th, 30th, 60th day. Histological studies of soft tissue samples were performed according to generally accepted methods. The growth factors content in serum was determined by enzyme-linked immunosorbent assay with the usage of bFGF (Elabscience) and VEGF (Vector-Best) kits. Results In EG1, the inflammatory reaction proceeded intensively and protractedly, which complicated the development and maturation of granulation tissue (GT). In EG2, considering the moderate inflammatory reactions, healing of the wound defect and optimal encapsulation of FB became possible. The increase of the growth factors content in the blood of Con group animals was maximal for a period of 15 days: bFGF – 2.2 times, VEGF – 1.6 times (p <0.001 compared to Int). In groups of animals with textile implants, the bFGF content was maximal on the 60th day and exceeded the normal values by 1.7 times in EG1and by 2.6 times in EG2 (p <0.001 compared to Int). The level of VEGF in EG1 and EG2 at all stages was slightly higher than in healthy animals.Conclusion At all stages of the experiment, the repair of damaged tissue in rats was complicated by the presence of the textile foreign body (TFB). This confirms the need for thorough debridement of combat wounds during primary surgical treatment. The increase in bFGF production contributed to the development and maturation of GT in the injury area and optimal encapsulation of FBs. The level of VEGF in the experimental groups was increased relative to normal values, which reflected the chronicity of the injuries regeneration process.

scholarly journals Healing features of experimental injuries of soft tissues that contain foreign bodies in the form of fragments of military personnel uniforms

2021 ◽  
pp. e001666
Author(s):  
Sergey Pavlov ◽  
O Litvinova ◽  
R Mikhaylusov ◽  
V Negoduyko ◽  
M Kumetchko ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Growth Factor ◽  
Foreign Bodies ◽  
Soft Tissues ◽  
Normal Values ◽  
Soft Tissue Injuries ◽  
Vascular Endothelial ◽  
Inflammatory Reactions ◽  
Endothelial Growth Factor

IntroductionThe healing of combat wounds can be complicated by the presence of foreign bodies. The present research aimed to study the regeneration of soft tissue injuries complicated by foreign bodies in the form of fragments of military uniforms.Methods60 rats were divided into four groups: intact (Int), control (Con) and two experimental (EG1, EG2). In Con, wounds were sutured without implantation of foreign bodies. A uniform consisting of 100% cotton was used as foreign bodies for EG1 and a uniform consisting of 65% cotton and 35% polyester for EG2. Histological studies were performed according to generally accepted methods. The growth factors wеre determined by ELISA.ResultsIn EG1, the inflammatory reaction proceeded protractedly, which complicated the development of granulation tissue. In EG2, considering the moderate inflammatory reactions, healing of the wounds became possible. The increase of the growth factors content in Con was maximal at the 15th day: basic fibroblast growth factor (bFGF)—2.2 times and vascular endothelial growth factor (VEGF)—1.6 times (p<0.001). In experimental groups, the bFGF content was maximal at the 60th day and exceeded the normal values by 1.7 times in EG1 and by 2.6 times in EG2 (p<0.001); the level of VEGF was slightly higher than that in healthy animals.ConclusionsThe study showed wound healing in all groups. The repair of injuries in rats of EG1 and EG2 was complicated by the textile implant and took longer. The dynamics of the content of growth factors reflected the impairment of wound healing processes. These violations were more pronounced in the EG1 group.

scholarly journals A CLINICAL CASE OF REMOVING A RESIDUAL FOREIGN BODY FROM THE LEFT SUPRACLAVICULAR REGION

2017 ◽  
Vol 4 (3) ◽  
pp. 120-122
Author(s):  
V.V. Boyko ◽  
V.V. Makarov ◽  
A.L. Sochnieva ◽  
V.V. Kritsak
Keyword(s):  
Surgical Treatment ◽  
Foreign Body ◽  
Soft Tissue ◽  
Clinical Case ◽  
Foreign Bodies ◽  
Soft Tissues ◽  
Life Quality ◽  
The Other ◽  
Practical Case ◽  
The One

Boyko V.V., Makarov V.V., Sochnieva A.L., Kritsak V.V.Residual foreign bodies in soft tissues are one of the main causes of chronical infection lesions and decrease in life quality. Surgical treatment is the most common way to relieve the patient from a foreign body. Often there is a question whether to remove a foreign body? On the one hand, all foreign bodies that are in the human body must be removed. On the other hand, in the absence of symptoms, the risk of surgery performed for the purpose of removal exceeds the risk associated with finding the foreign body. We would like to describe a practical case of removing a foreign body (Kirschner`s wires) from the left supraclavicular region. The young patient lived with a fragment of Kirschner's wire left after the osteosynthesis of the fractured clavicle for 5 years. Surgery to remove the residual foreign body was successful. On the 7th postoperative day the patient was discharged from the hospital under the supervision of surgeons at the place of residence.Key words: foreign body in soft tissue, Kirschner`s wire, surgical treatment. КЛІНІЧНИЙ ВИПАДОК ВИДАЛЕННЯ ЗАЛИШКОВ СТОРОННЬОГО ТІЛА З ЛІВОЇ НАДКЛЮЧИЧНОЇ ОБЛАСТІБойко В.В., Макаров В.В., Сочнева А.Л.,  Крицак В.В.Залишкові чужорідні тіла м'яких тканин залишаються однією з основних причин виникнення вогнища хронічної інфекції та зниження рівня якості життя. Хірургічне лікування основний спосіб позбавити хворого від наявності чужорідного агента. Часто виникає питання чи видаляти чужорідне тіло. З одного боку, усі сторонні тіла, що знаходяться в тілі людини, підлягають видаленню, з іншого боку при відсутності симптомів ризик операції, проводимої з метою видалення, перевищує ризик, пов'язаний з перебуванням чужорідного тіла. Ми хотіли б поділитися випадком видалення залишкового стороннього тіла (спиці Кіршнера) лівої надключичної ділянки із власної практики. Молода пацієнтка прожила з уламком спиці Кіршнера, залишеної після металлоостеосинтезу поламаної ключиці протягом 5 років. Операція з видалення залишкового стороннього тіла пройшла успішно. На 7 післяопераційну добу пацієнтка була виписана зі стаціонару під спостереження хірурги за місцем проживання.Ключові слова: чужорідне тіло м'яких тканин, спиця Кіршнера, хірургічне лікування. кЛИНИЧЕСКИЙ СЛУЧАЙ УДАЛЕНИЯ ОСТАТКОВ ИНОРОДНОГО ТЕЛА ИЗ ЛЕВОЙ ПОДКЛЮЧИЧНОЙ ОБЛАСТИ Бойко В.В., Макаров В.В., Сочнева А.Л.,  Крицак В.В.Остаточные инородные тела мягких тканей остаются одной из основных причин возникновения очага хронической инфекции и снижения уровня качества жизни. Хирургическое лечение основной способ избавить больного от наличия чужеродного агента. Часто возникает вопрос удалять ли инородное тело? С одной стороны, все инородные тела, находящиеся в теле человека, подлежат удалению, с другой стороны при отсутствии симптомов риск операции, производимой с целью удаления, превышает риск, связанный с нахождением инородного тела. Мы хотели бы поделится случаем удаления остаточного инородного тела (спицы Киршнера) левой надключичной области из собственной практики. Молодая пациентка прожила с обломком спицы Киршнера, оставленной после металлоостеосинтеза поломанной ключицы в течении 5 лет. Операция по удалению остаточного инородного тела прошла успешно. На 7 послеоперационные сутки пациентка была выписана из стационара под наблюдение хирурги по месту жительства.Ключевые слова: инородное тело мягких тканей, спица Киршнера, оперативное лечение.

Platelet-Rich Plasma Powder: A New Preparation Method for the Standardization of Growth Factor Concentrations

2016 ◽  
Vol 45 (4) ◽  
pp. 954-960 ◽  
Author(s):  
Matthias Kieb ◽  
Frank Sander ◽  
Cornelia Prinz ◽  
Stefanie Adam ◽  
Anett Mau-Möller ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Clinical Application ◽  
Whole Blood ◽  
Sports Medicine ◽  
Transforming Growth Factor ◽  
Platelet Rich Plasma ◽  
Transforming Growth Factor Β1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tgf Β1

Background: Platelet-rich plasma (PRP) is widely used in sports medicine. Available PRP preparations differ in white blood cell, platelet, and growth factor concentrations, making standardized research and clinical application challenging. Purpose: To characterize a newly standardized procedure for pooled PRP that provides defined growth factor concentrations. Study Design: Controlled laboratory study. Methods: A standardized growth factor preparation (lyophilized PRP powder) was prepared using 12 pooled platelet concentrates (PCs) derived from different donors via apheresis. Blood samples and commercially available PRP (SmartPrep-2) served as controls (n = 5). Baseline blood counts were analyzed. Additionally, single PCs (n = 5) were produced by standard platelet apheresis. The concentrations of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), platelet-derived growth factor AB (PDGF-AB), transforming growth factor β1 (TGF-β1), insulin-like growth factor 1 (IGF-1), interleukin (IL)–1α, IL-1β, and IL-1 receptor agonist (IL-1RA) were analyzed by enzyme-linked immunosorbent assay, and statistical analyses were performed using descriptive statistics, mean differences, 95% CIs, and P values (analysis of variance). Results: All growth factor preparation methods showed elevated concentrations of the growth factors VEGF, bFGF, PDGF-AB, and TGF-β1 compared with those of whole blood. Large interindividual differences were found in VEGF and bFGF concentrations. Respective values (mean ± SD in pg/mL) for whole blood, SmartPrep-2, PC, and PRP powder were as follows: VEGF (574 ± 147, 528 ± 233, 1087 ± 535, and 1722), bFGF (198 ± 164, 410 ± 259, 151 ± 99, and 542), PDGF-AB (2394 ± 451, 17,846 ± 3087, 18,461 ± 4455, and 23,023), and TGF-β1 (14,356 ± 4527, 77,533 ± 13,918, 68,582 ± 7388, and 87,495). IGF-1 was found in SmartPrep-2 (1539 ± 348 pg/mL). For PC (2266 ± 485 pg/mL), IGF-1 was measured at the same levels of whole blood (2317 ± 711 pg/mL) but was not detectable in PRP powder. IL-1α was detectable in whole blood (111 ± 35 pg/mL) and SmartPrep-2 (119 ± 44 pg/mL). Conclusion: Problems with PRP such as absent standardization, lack of consistency among studies, and black box dosage could be solved by using characterized PRP powder made by pooling and lyophilizing multiple PCs. The new PRP powder opens up new possibilities for PRP research as well as for the treatment of patients. Clinical Relevance: The preparation of pooled PRP by means of lyophilization may allow physicians to apply a defined amount of growth factors by using a defined amount of PRP powder. Moreover, PRP powder as a dry substance with no need for centrifugation could become ubiquitously available, thus saving time and staff resources in clinical practice. However, before transferring the results of this basic science study to clinical application, regulatory issues have to be cleared.

scholarly journals Periradicular Tissue Responses to Biologically Active Molecules or MTA When Applied in Furcal Perforation of Dogs' Teeth

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Anna Zairi ◽  
Theodoros Lambrianidis ◽  
Ourania Pantelidou ◽  
Serafim Papadimitriou ◽  
Dimitrios Tziafas
Keyword(s):  
Zinc Oxide ◽  
Growth Factors ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Mineral Trioxide Aggregate ◽  
Biologically Active ◽  
Inflammatory Reactions ◽  
Zinc Oxide Eugenol ◽  
Tissue Responses ◽  
Igf I

The aim of this study was the comparative evaluation of inflammatory reactions and tissue responses to four growth factors, or mineral trioxide aggregate (MTA), or a zinc-oxide-eugenol-based cement (IRM) as controls, when used for the repair of furcal perforations in dogs’ teeth. Results showed significantly higher inflammatory cell response in the transforming growth factorβ1 (TGFβ1) and zinc-oxide-eugenol-based cement (IRM) groups and higher rates of epithelial proliferation in the TGFβ1, basic fibroblast growth factor (bFGF), and insulin growth factor-I (IGF-I) groups compared to the MTA. Significantly higher rates of bone formation were found in the control groups compared to the osteogenic protein-1 (OP-1). Significantly higher rates of cementum formation were observed in the IGF-I and bFGF groups compared to the IRM. None of the biologically active molecules can be suggested for repairing furcal perforations, despite the fact that growth factors exerted a clear stimulatory effect on cementum formation and inhibited collagen capsule formation. MTA exhibited better results than the growth factors.

scholarly journals Ultrasound-guided removal of soft tissue foreign bodies in companion animals: A case series

2020 ◽  
Vol 65 (No. 2) ◽  
pp. 49-55
Author(s):  
S Manfredi ◽  
G Covi ◽  
M Bonazzi ◽  
G Gnudi ◽  
M Fumeo ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Minimally Invasive ◽  
Foreign Bodies ◽  
Soft Tissues ◽  
Companion Animals ◽  
Case Series ◽  
Invasive Technique ◽  
Surgical Removal ◽  
Small Animals ◽  
Ultrasound Guided

Foreign bodies (FBs) retained in the subcutaneous tissues are a common reason for medical consultation. In small animals, FBs usually consist of vegetal materials, especially grass awns. Failure to remove the FBs is likely to give rise to acute or late complications. The surgical removal of the FBs can be invasive, costly and technically challenging. Ultrasound has become a mainstay in the detection of FBs and it can be used to guide the extraction of the FBs with a minimally invasive technique. This study describes the detection and extraction of soft-tissue FBs in small animals. One hundred-sixty-two patients, presenting at two veterinary clinics with suspected FBs retained in the soft tissues of various body districts, were considered. Once an ultrasound diagnosis was established, the ultrasound-guided removal of the FB was performed. A high-frequency linear transducer, a skin disinfection, sedation or anaesthesia was used when needed and a scalpel and some Hartmann forceps were also used. One hundred-eighty-two FBs were successfully removed in all the patients. In six cases, the FB was identified during a second ultrasonographic examination, after recurrence of the fistula. No complications were reported after the procedure. The extraction of the FB was performed in an echographic suite in 138 cases and in a surgery room with surgical intervention in 24 cases. In the latter situation, the surgical minimally invasive dissection of tissues under ultrasound guidance was performed before the removal of the FB. In conclusion, the ultrasound-guided removal of the FBs retained in the superficial soft tissue can be considered a good alternative to surgery. However, failure to remove a FB does not preclude the removal by traditional surgery.

scholarly journals ENCAPSULATION OF METALLIC SOFT-TISSUE FOREIGN BODIES WITH A LONG CARRIER LIFE

2020 ◽  
pp. 160-164
Author(s):  
V. V. Negoduyko ◽  
R. M. Mikhailusov ◽  
T. P. Yakimova ◽  
P. M. Zamyatin ◽  
S. O. Beresnev ◽  
...  
Keyword(s):  
Foreign Body ◽  
Soft Tissue ◽  
Foreign Bodies ◽  
Soft Tissues ◽  
Clinical Laboratory ◽  
Gunshot Wounds ◽  
Analysis Data ◽  
Capsule Formation ◽  
Iron And Steel ◽  
Morphological Studies

Sumary. The aim is to investigate the features of pathogenesis in capsule formation around metallic foreign bodies of soft tissues. Materials and methods. The results of a study of 6 patients with metallic foreign bodies of soft tissues with a carrier period of 16 to 50 years were analyzed. 3 injured had foreign bodies of gunshot origin and 3 patients had foreign bodies as a result of personal injury. Used clinical, laboratory, instrumental, histological, immunohistochemical, X-ray spectrometric studies. Results. All foreign soft tissue bodies were removed with the capsule. By gender: 5 men and 1 woman. By localization - the lower extremity. Pain was present in all the victims. Radiography and ultrasound were informative, magnetodetection is informative only at the superficial location of a foreign body. The foreign bodies were made of gray cast iron and steel needle wire. Histologically, immunohistochemically and radiospectrometrically, it has been found that the capsule formation around the foreign body is affected by the mechanism and extent of damage to the soft tissues, the composition of the metal and its coating, and the carrier term of the foreign body. Conclusions. Depending on the composition of the metal and its coating, there is a different rate of oxidation of the foreign body in the soft tissues: oxidation of metal foreign bodies of fire origin is faster. The formation of the capsule around the foreign body is affected by the mechanism of tissue damage: when the needle penetrates, the soft tissues are destroyed minimally, in the case of gunshot wounds, they are destroyed more. Spectral analysis data in conjunction with morphological studies are the basis for the removal of a foreign body of inflammatory origin along with the capsule.

Selective receptor-mediated impairment of growth factor activity in neonatal- and X-linked adrenoleukodystrophy patients

2019 ◽  
Vol 32 (7) ◽  
pp. 733-738
Author(s):  
Mazen Al-Essa ◽  
Gursev S. Dhaunsi
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Dna Synthesis ◽  
Serum Levels ◽  
Vital Role ◽  
Brdu Incorporation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Factor Activity ◽  
Poor Growth ◽  
Fgf Receptor

Abstract Background Neonatal adrenoleukodystrophy (n-ALD) and X-linked ALD (X-ALD) patients present with demyelination, poor growth and progressive mental retardation. Growth factors are known to play a vital role in the development of children. Objective To examine the mitogenic activity of various growth factors in skin fibroblasts from n-ALD and X-ALD patients. Methods Skin fibroblast cultures from n-ALD and X-ALD patients, and controls were treated with 50 ng/mL of platelet-derived growth factor (PDGF), basic fibroblast growth factor (bFGF) or insulin-like growth factor-1 (IGF-1) to examine DNA synthesis by 5-bromo-2′-deoxyuridine (BrdU) incorporation. Expression of receptors for PDGF, bFGF and IGF-1 was measured by western blotting. Serum levels of IGF-1 were assayed by enzyme-linked immunosorbent assay (ELISA). Results Fibroblasts from n-ALD and X-ALD patients had significantly (p < 0.01) less BrdU incorporation in response to fetal bovine serum (FBS). The mitogenic effect of PDGF, bFGF and IGF-1 was significantly lower in n-ALD as compared to control and X-ALD cells. X-ALD cells showed significant impairment in IGF-1-induced DNA synthesis. Expression of the FGF receptor (FGF-R) was significantly reduced in n-ALD cells. PDGF receptor remained unaffected, and IGF-1 receptor (IGF-1R) expression and serum IGF-1 levels were significantly (p < 0.01) reduced in n-ALD and X-ALD patients as compared to controls. Conclusions Growth factor activity differs in n-ALD and X-ALD patients, with marked impairment of IGF-1 function through receptor down-regulation.

Angiogenic Growth Factors: Their Effects and Potential in Soft Tissue Wound Healing

1992 ◽  
Vol 101 (4) ◽  
pp. 349-354 ◽  
Author(s):  
David B. Hom ◽  
Robert H. Maisel
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Growth Factor ◽  
Soft Tissue ◽  
Transforming Growth Factor Beta ◽  
Animal Studies ◽  
Transforming Growth Factor ◽  
Therapeutic Potential ◽  
Angiogenic Growth Factors

Since their discovery 30 years ago, angiogenic growth factors have been demonstrated to stimulate neovascularization in vitro and in animal studies. Over the last decade, knowledge gained in the field of angiogenic growth factors has grown immensely. These angiogenic growth factors exist in four major families: fibroblast growth factor (FGF), transforming growth factor beta (TGF-β), platelet-derived growth factor (PDGF), and epidermal growth factor (EGF). Each has the ability to induce soft tissue vascularization in microgram quantities. In animal models, FGF, TGF-β, PDGF, and EGF have been shown to enhance soft tissue wound healing. In human clinical trials, EGF and a mixture of PDGFs have been demonstrated to accelerate epidermal regeneration in cutaneous wounds. These factors have considerable therapeutic potential in the areas of soft tissue wound healing and otolaryngology. This article reviews important aspects of angiogenic growth factors and discusses their future potential in soft tissue wound healing.

scholarly journals Retained Metallic Foreign Body in Cheek: A Diagnostic and Surgical Challenge

2013 ◽  
Vol 4 (2) ◽  
pp. 107-109
Author(s):  
Varsha Sunil Manekar ◽  
Ankush Chavan
Keyword(s):  
Foreign Body ◽  
Soft Tissue ◽  
Unusual Case ◽  
Foreign Bodies ◽  
Soft Tissues ◽  
Metallic Foreign Body ◽  
Industrial Accidents ◽  
Traumatic Injuries ◽  
Orofacial Region ◽  
Head Neck

ABSTRACT Foreign body lodged in the soft tissue is fairly common in the vehicular or industrial accidents. Traumatic injuries in orofacial region often drive foreign bodies in the soft tissues. The immediate closure of the soft tissue wounds become the emergency treatment for the control of bleeding. The foreign body may sometimes remain unnoticed. The purpose of reporting this unusual case of metallic foreign object in the cheek is to highlight the difficulties in detection of foreign bodies and discuss its clinical management. We also discuss the usefulness of various imaging modalities for assessment of its nature, location, size, shape and relation to vital structures. How to cite this article Manekar VS, Chavan A. Retained Metallic Foreign Body in Cheek: A Diagnostic and Surgical Challenge. Int J Head Neck Surg 2013;4(2):107-109.

scholarly journals Foreign Body (Bamboo Splinter of Broom Stick) in Soft Tissue

1970 ◽  
Vol 20 (1) ◽  
pp. 67-70 ◽  
Author(s):  
MA Kasem Pramanik ◽  
Joydeep Bhaduri ◽  
AM Rashid ◽  
M Nazmul Hasan
Keyword(s):  
Foreign Body ◽  
High Resolution ◽  
Soft Tissue ◽  
Foreign Bodies ◽  
Soft Tissues ◽  
Cost Effective ◽  
Daily Practice ◽  
Radiation Hazard ◽  
X Ray ◽  
High Resolution Ultrasonography

Patients with foreign bodies inside soft tissues are common in a surgeon's daily practice,. Radio-opaque foreign bodies can easily be located with radiography but radio-lucent foreign bodies cannot be located with X-ray, where Ultrasonography especially, high resolution ultrasonography can be used to locate it. Ultrasonography, being easily available, cost-effective and radiation-hazard free, can be done repeatedly for foreign bodies which move inside tissues. The presenting article describes a patient with a radio-lucent foreign body, deep inside muscle in his fore-arm and having one sharp end, was advancing inside tissues, was located with the help of Ultrasonography and was removed.   doi: 10.3329/taj.v20i1.3095 TAJ 2007; 20(1): 67-70

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