Transcriptomic Analysis of Quinoa Reveals A Group of Germin-Like Proteins Induced By Trichoderma
Abstract Background: Fungi in the Trichoderma genus affect growth and pathogen resistance of many plant species with different outcomes. Most plant-Trichoderma interactions result in a beneficial relationship. However, Trichoderma fungi may have a negative impact on certain plants depending on their genotype. Thus, plant-Trichoderma interactions outcome might depend on their genetic compatibility, which is not known in molecular detail. Results: Here we describe the transcriptomic response of two cultivars of Chenopodium quinoa to axenic co-cultivation with Trichoderma harzianum BOL-12 and Trichoderma afroharzianum T22. The response of C. quinoa roots to BOL-12 and T22 in the early phases of interaction was studied by RNA sequencing and RT-qPCR verification. Interaction with the two fungal strains induced partially overlapping gene expression responses. Comparing the two plant genotypes, a broad spectrum of putative quinoa defense genes were found activated in cultivar Kurmi but not in the Real cultivar. In cultivar Kurmi, relatively small effects were observed for classical pathogen response pathways but instead a C. quinoa-specific clade of putative defensive germin-like genes were activated. Germin-like genes were found to be more rapidly induced in cultivar Kurmi as compared to Real. The same germin-like genes were found to be upregulated systemically in Kurmi leaves. No strong correlation was observed between any of the known hormone-mediated defense response pathways and any of the quinoa-Trichoderma interactions. Conclusions: C. quinoa triggers a set of germin-like defensive genes in response to Trichoderma interaction. Quinoa germin-like gene expression is cultivar-specific upon interaction with Trichoderma and was found to be expressed also systemically. The observed differences of quinoa response to Trichoderma for each quinoa cultivar are relevant for the application of Trichoderma agents for quinoa crop protection.