scholarly journals TLC BASED PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF SENNA ALATA

2020 ◽  
Vol 8 (11) ◽  
pp. 1099-1107
Author(s):  
Rajeshwari Prabha Lahare ◽  
◽  
Yogesh Kumar Bisen ◽  
Hari Shankar Yadav ◽  
Anil Kumar Dashahre ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Skin Diseases ◽  
Antimicrobial Properties ◽  
Bioactive Molecules ◽  
Flowering Plant ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Tlc Analysis ◽  
Insect Bites ◽  
Senna Alata

Senna alata is an ornamental flowering plant also known as ringworm bush and candle bush belongs to Leguminaceae family and used mainly as antifungal agent .The plant is pharmacological significant and used as antibacterial, antioxidant, anti-cancerous, anti-mutagenic, antifungal, anti-diabetic and antiviral activities. Leaves of the plant have anti-parasitic activity used in treatment of eczema, ringworm, asthma, bronchitis and in poisonous insect bites, bark of the plant is used in treatment of skin diseases. In the present study chloroform, methanol, petroleum ether and aqueous extracts were used for phytochemical analysis, TLC profiling and antioxidant activity of Senna alata. The phytochemical analysis of Senna alata indicated presence of tannins, saponins, flavonoids, alkaloids, terpenoids, steroids, cardiac glycosides and quinones in Senna alata. In TLC analysis maximum 6 bands were found in leaf extract, 5 bands in stem extract and 4 bands were found in root methanol extract. The plant showed good antioxidant activity in methanol and aqueous based leaf, stem and root extract which may be due to the presence of flavonoids and phenolic compounds. The plant has potent antioxidant and antimicrobial properties so characterization of bioactive molecules of the plant should be beneficial for formulation of drugs.

scholarly journals Phytochemical, antioxidant and antimicrobial properties of Litsea angulata extracts

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1839
Author(s):  
Harlinda Kuspradini ◽  
Indah Wulandari ◽  
Agmi Sinta Putri ◽  
Sabeti Yulis Tiya ◽  
Irawan Wijaya Kusuma
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Phytochemical Analysis ◽  
Dpph Radical ◽  
Free Radical Formation ◽  
Dpph Radical Scavenging ◽  
Phytochemical Constituent ◽  
Microdilution Broth

Background: Litsea angulata is a plant species belonging to Lauraceae family that is distributed throughout Indonesia, Malaysia, and New Guinea. The seeds have been traditionally used by local people in Kalimantan, Indonesia for the treatment of boils; however, there is no information about the potency of its branch, bark and leaves yet. This study aimed to determine the antioxidant, antimicrobial activity as well as the phytochemical constituent of Litsea angulata branch, bark, and leaves. Methods: Extraction was performed by successive maceration method using n-hexane, ethyl acetate, and ethanol solvent. Antioxidant activity was evaluated by DPPH radical scavenging assay. The antimicrobial activity using the 96 well-plate microdilution broth method against Staphylococcus aureus and Streptococcus mutans. Results: Based on the phytochemical analysis, it showed that extract of L. angulata contains alkaloids, flavonoids, tannins, terpenoids, and coumarin. The results showed that all extracts of plant samples displayed the ability to inhibit DPPH free radical formation and all tested microorganisms. Conclusions: L. angulata contains secondary metabolites such as alkaloids, flavonoids, tannins, terpenoids, carotenoids, and coumarin. The antioxidant activity on different plant extracts was a range as very strong to weak capacity. All extracts in this study could inhibit the growth of S. aureus and S. mutans.

scholarly journals Qualitative Phytochemical Analysis and Antioxidant Activity of Methonolic Extract of Eichhornia crassipes (Mart.) Solms and Pistia

2017 ◽  
Vol 9 (5) ◽  
Author(s):  
Tyagi Tulika ◽  
Parashar Puneet ◽  
Agarwal Mala
Keyword(s):  
Antioxidant Activity ◽  
Water Hyacinth ◽  
Eichhornia Crassipes ◽  
Antimicrobial Properties ◽  
New Drugs ◽  
Pistia Stratiotes ◽  
Ayurvedic Medicine ◽  
Phytochemical Analysis ◽  
Good Source ◽  
Invasive Weed

Eichhornia crassipes (Mart.) solms and Pistia stratiotes (L.) are two invasive weed aquatic plants that have been traditionally known as “water hyacinth” and “Jalkumbhi” respectively. They are commonly used in Ayurvedic medicine which possesses diuretic, antidiabetic, antidermatophytic, antifungal, and antimicrobial properties. The present study was carried out to estimate the total phytochemicals such as phenolics, flavonoids, tannins, alkaloids, protein, carbohydrate, lipid, amino acids and antioxidant activity. The presence of various phytochemicals in the plants reveals that these plants may be good source for the production of new drugs for various ailments.

Evaluation of antioxidant and antibacterial capacity of green microalgae Scenedesmus subspicatus

2019 ◽  
Vol 25 (4) ◽  
pp. 318-326 ◽  
Author(s):  
Danielli Matias de Macedo Dantas ◽  
Carlos Yure Barbosa de Oliveira ◽  
Romero Marcos Pedrosa Brandão Costa ◽  
Maria das Graças Carneiro-da-Cunha ◽  
Alfredo Olivera Gálvez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Dimethyl Sulfoxide ◽  
Raw Materials ◽  
Antimicrobial Properties ◽  
Human Consumption ◽  
Phytochemical Analysis ◽  
Toxic Substances ◽  
Green Microalgae ◽  
Scenedesmus Subspicatus

Microalgae are considered one of the most promising raw materials for the development of high value products for pharmaceuticals, nutraceuticals, and cosmetic industries, as well as being potential sources of protein, vitamins, and minerals for human consumption. Hence, the present research focuses extraction of antioxidant and antimicrobial compounds from Scenedesmus subspicatus using solvents of different polarities. Different solvents such as ethanol, methanol, butanol, acetone, dimethyl sulfoxide, and water were used to extract compounds from the green microalgae S. subspicatus and then they were examined for phytochemical screening, antioxidant activity, and antimicrobial properties. In vitro free radical quenching and total antioxidant activity of extracts were investigated with 1,1-diphenyl-2-picryl hydrazyl and compared with catequin and gallic acid as positive controls. The antimicrobial activity was evaluated in gram-negative and gram-positive bacteria. Aqueous extracts and dimethyl sulfoxide presented better performance in phytochemical analysis. This result showed consistency in the sequential tests. The antioxidant activity was also better using the two solvents cited above. The extracts acetone, water, and dimethyl sulfoxide showed ability to inhibit the growth of Bacillus subtilis. However, only dimethyl sulfoxide inhibited the growth of Klebsiella pneumoniae and Escherichia coli. Use of the aqueous extract, proven its effectiveness, is an economic protocol and avoids the use of toxic substances.

Pesudomance sp. Bacteria Associated with Marine Sponge as a Promising and Sustainable Source of Bioactive Molecules

2019 ◽  
Vol 20 (11) ◽  
pp. 964-984 ◽  
Author(s):  
Asmaa Nabil-Adam ◽  
Mohamed A. Shreadah ◽  
Nehad M. Abd El Moneam ◽  
Samy A. El-assar
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Marine Sponge ◽  
Bacterial Strain ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Bioactive Molecules ◽  
Phytochemical Analysis ◽  
Anti Inflammatory

Background: The study was conducted to identify the bacterial strain associated with marine sponge Hyrtiosaff. erectus collected from the Red Sea coastal water and to assess the utilization of their secondary metabolites for human benefit as antioxidant, anti-Alzheimer, anti-viral, anticancer and anti-inflammatory agent. Methods: After biochemical identification of Pesudomance sp. bacterial strain, the total polyphenol contents, cytotoxic, antioxidant, anti-Alzheimer, anti-viral, anticancer and anti-inflammatory activity of the Pesudomance sp. ethyl acetate extract were investigated by applying different biochemical assays. Polyphenol contents were investigated using spectrophotometric techniques. Antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), and 2,2/-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) ABTS radical scavenging activity assays. The cytotoxic effects were investigated by using the human cancerous cell lines. Results: The anti-Alzheimer, anti-viral, anticancer and anti-inflammatory activities were determined using ELISA. Qualitative phytochemical analysis of the Pesudomance sp. extract demonstrated the presence of a large and diverse group of substances such as alkaloids, carbohydrates, flavonoids, phenols, terpenoids, saponins, and tannins. The strong antioxidant activity of the Pesudomance sp. extract was mainly attributed to the protective role of polyphenols against reactive oxygen. It was also observed that Pesudomance sp. extract possessed significant anti-Alzheimer activity with 94% at 1 mg. The extract showed also high antiviral activity (90%) using reverse transcriptase enzymes inhibition assay. The examination of the anticancer activity by applying two experimental models, i.e., PTK and SHKI cleared out high significant percentages of 76.19 and 83.09 %; respectively. Conclusion: The anti-inflammatory profiling using TNF, COX1, COX2, IL6 also revealed high antiinflammatory activity with different metabolic pathway of 62.70, 75.444, 79.27 and 54.15 %; respectively. The present study concluded that ethyl acetate extract of Pesudomance sp. possessed strong antioxidant, anti-Alzheimer, and anti-viral, anticancer and anti-inflammatory activities. Further studies are required to purify the bioactive compounds.

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT PROPERTIES BY DPPH RADICAL SCAVENGER ACTIVITY OF RUELLIA BRITTONIANA FLOWER

2019 ◽  
pp. 24-28
Author(s):  
NADZILA ANINDYA TEJAPUTRI ◽  
ADE ARSIANTI ◽  
FONA QORINA ◽  
QOTRUNNADA FITHROTUNNISA
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Chemical Components ◽  
Phytochemical Analysis ◽  
Radical Scavenger Activity ◽  
Scavenger Activity ◽  
Dpph Method ◽  
Tlc Analysis

Objective: The genus Ruellia has been widely used in traditional and Ayurvedic medicine as an antioxidant. This study seeks to examine the antioxidant activity of the species Ruellia brittoniana. Methods: In this study, Ruellia brittoniana flowers were acquired from Depok, West Java, Indonesia. The flowers were cleaned and ground to form a powder, then dissolved in hexane, ethanol and ethyl acetate solvents. These three extracts were then tested for phytochemicals and thin layer chromatography (TLC) analysis. Ethanol and ethyl acetate extracts were also analyzed for antioxidants using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Results: Phytochemical results from the three extracts proved that Ruellia brittoniana contains flavonoids, alkaloids, tannins, glycosides and triterpenes. These results are comparable to the results from TLC analysis, which showed the samples contained 4–5 chemical components. Furthermore, the best antioxidant activity resulted from the ethyl acetate extract of the Ruellia brittoniana flower with an IC50 value of 68.42 ppm. Conclusion: An ethyl acetate extract from the Ruellia brittoniana flower can be used as a natural source of additional antioxidants.

scholarly journals Characterisation of an Aromatic Plant-based Formula using UV-Vis Spectroscopy, LC–ESI(+)QTOF-MS and HPLC-DAD Analysis

2013 ◽  
Vol 70 (1) ◽  
pp. 16 ◽  
Author(s):  
Florina Bunghez ◽  
Carmen Socaciu ◽  
Florina Zagrean ◽  
Raluca Maria Pop ◽  
Floricuta Ranga ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Biological Effects ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
New Product ◽  
Bioactive Molecules ◽  
Accurate Information ◽  
Vis Spectroscopy ◽  
The Individual

Abstract. It is known for a long time that seasoning/condimentary herbs have antioxidant activity and antibacterial properties, being good natural alternatives for disease prevention. The different efficiency of these plants is assigned to their bioactive molecules, stability and bioavailability. In the present study seven aromatic herbs (basil, thyme, oregano, rosemary, clove, cinnamon and sage) were investigated individually. A new product was developed using basil, thyme, oregano, rosemary, clove, cinnamon and sage, according to a default recipe. The characterization of each plant aimed to identify the specific “fingerprint” by its main bioactive molecules and the “traceability” of these molecules in the new product, made by mixing the selected plants according to a default recipe. In order to determine the main bioactive compounds of the individual plants composition, in comparison with the new plant-based (EPC) formula, high throughput techniques like UV-Vis spectroscopy and LC-QTOF-MS  spectrometry were used. The most important bioactive compounds determined in the studied herbs, which may exert antioxidant activity and antibacterial properties, were phenolic compounds (phenolic acids, flavonoids), quinones, clorophylls as well some polar terpenoids. The fingerprints are providing comprehensive and accurate information about the compounds that may exert antimicrobial properties. In order to assure the biological effects and the bioavailability of the polyphenols and the secondary metabolites we have to consider the antagonistic and synergistic effect that the metabolites can exert on each other.

scholarly journals Phytochemical and Antimicrobial Properties of the Aqueous Root Extract of Triclisia dictyophylla

2013 ◽  
Vol 4 (1) ◽  
pp. 15-20
Author(s):  
Anslem O Ajugwo ◽  
Anthony C Ezimah ◽  
Francis M Awah ◽  
Philippe E Mounbegna ◽  
Celestine C Azikiwe
Keyword(s):  
Staphylococcus Aureus ◽  
Antifungal Activity ◽  
Antimicrobial Properties ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Medical Sciences ◽  
Active Components ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Aqueous Root Extract

Triclisia dictyophylla have been used traditionally for the treatment of different ailments. The root was therefore subjected to phytochemical analysis and antimicrobial/antifungal activity against some hospital-strain disease causing microorganisms. Standard methods were used for the phytochemical screening. The extract was subjected to antimicrobial/antifungal activity using Staphylococcus aureus, Escherichia coli, Klebsiella spp, Pseudomonas aeruginosa and Candida albicans. The sensitivity pattern was compared to that of ciprofloxacin, cotrimoxazole and ofloxacin. Phytochemical analysis revealed mostly alkaloids and protein while tannins, glycoside and saponin were revealed in trace (+) amounts. The extract showed a good antimicrobial activity on Staphylococcus aureus isolated from sputum and on E. coli from both urine and sputum with no antifungal activity. The extract contains active components which could be harnessed for formulation of antibiotics.DOI: http://dx.doi.org/10.3126/ajms.v4i1.7846   Asian Journal of Medical Sciences 4(2013) 15-20  

PHENOLIC CONTENT AND POTENTIAL APPLICATION OF „LYSIMACHIA VULGARIS“ L. AERIAL PART AND ROOT EXTRACTS

2021 ◽  
Author(s):  
Nikola Z. Srećković ◽  
◽  
Jelena S. Katanić Stanković ◽  
Vladimir B. Mihailović
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Potential Application ◽  
Aerial Part ◽  
Phenolic Content ◽  
Flowering Plant ◽  
Total Phenolic ◽  
Root Extract ◽  
High Level

Lysimachia vulgaris L., also known as yellow loosestrife, is an herbaceous perennial flowering plant from the family Primulaceae. Traditionally, L. vulgaris has been commonly used in Chinese medicine to increase urination, reduce fever, and treat sexually transmitted ailments. This study aimed to investigate phenolic content, antioxidant activity, and antimicrobial potential of L. vulgaris aerial part (LVA) and root (LVR) methanol extracts. Based on the spectrophotometric determination, both extracts possessed a high level of phenolic compounds. The content of total phenolic compounds (130.14 mg GAE/g) and condensed tannins (120.58 mg GAE/g) was higher in LVR, while total flavonoid (206.93 mg QUE/g) and flavanol (17.02 RUE/g) contents were higher in LVA. DPPH and ABTS assays were used to estimate the antioxidant activity of the extracts, whereby LVR showed better antioxidant potential in both applied methods. The extracts inhibited the growth of most of the tested bacterial and fungal strains with minimal inhibitory concentration values (MICs) ranged between (˂ 0.3125 to 20 mg/mL), whereby the significantly higher antimicrobial activity of LVR was observed. Based on the obtained results, the significant antioxidant and antimicrobial activity of L. vulgaris root extract may be a basis for its further research and potential application in the pharmaceutical and food industry.

Anti-microbial, antioxidant potential of Cassia fistula and study their phytochemical assessments

2021 ◽  
Vol 7 (2) ◽  
pp. 130-140
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Salmonella Typhi ◽  
Flowering Plant ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Cassia Fistula

Cassia fistula (C. fistula) is a flowering plant and a member of Fabaceae family. This study was designed to examine the antibacterial, antioxidant and phytochemical activity of ethanolic extract of C. fistula plant. The microbial inhibitory effect of ethanolic extracts of C. fistula was tested against Gram positive isolates such as Bacillus cereus, Staphylococcus aureus and Gram-negative isolates such as Salmonella typhi, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa using disc diffusion method and well diffusion method. The 25 mg extract of C. fistula leaves (CF-05) showed more zone of inhibition against Salmonella typhi, i.e., (21mm) and in 50 mg extract of CF-13 fruit showed best zone of inhibition against Salmonella typhi, i.e., 17 mm. Qualitative analysis and antioxidant activity at various concentrations was also measured. The phytochemical analysis showed the presence of alkaloids, carbohydrates, fats, tannins, flavonoids, saponins, terpenoids, and sterols. The antioxidant activity in 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity revealed the distinguished antioxidant activity of C. fistula.

scholarly journals Uji Aktivitas Antioksidan Ekstrak n-Heksana, Etil Asetat, Metanol Daun dan Akar Bakau Merah (Rhyzophora stylosa) dengan Metode DPPH

ALCHEMY ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 20
Author(s):  
Ahmad Hanapi ◽  
Ahmad Ghanaim Fasya ◽  
Abdan Syakuro
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Mangrove Species ◽  
Rhizophora Stylosa ◽  
Red Mangrove ◽  
Dpph Method ◽  
Leaves And Roots

<p>Red mangrove (<em>Rhizophora stylosa</em>) is one of the mangrove species which is abundance in the coast of Java. The mangrove contains a lot of active compounds that is potent as antioxidant. The objective of this study was to determine antioxidant activity of n-hexane, ethyl acetate, and methanol extract of red mangrove leaves and roots using DPPH (1,1-diphenyl-2-picrylhydrazyl) method. Active compound extraction of red mangrove is conducted by gradual maceration. According to phytochemical analysis, crude root extract showed the presence of flavonoids, tannins, and steroids/triterpenoids, while crude leave extract showed the presence of alkaloids, flavonoids, tannins, saponins, and steroids/triterpenoids. EC<sub>50</sub> value of methanol extract from red mangrove leaves and roots was 5.01 and 2.55 ppm, respectively, whilst of ethyl acetate extracts from red mangrove leaves and roots was 89.94 and 8.51 ppm, respectively. Crude extract n-hexane has antioxidant activity 33.14 ppm (EC<sub>50</sub>). Roots and leaves extracts have high activity of antioxidant.</p><p> </p><p>Keywords: Antioxidant, red mangrove, DPPH method</p><p> </p><p> </p><p>Bakau merah (<em>Rhizophora stylosa</em>) merupakan salah satu spesies bakau yang keberadaannya sangat melimpah di pesisir pantai Pulau Jawa dan mengandung banyak senyawa aktif yang berpotensi sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui aktifitas antioksidan ekstrak n-heksana, etil-asetat, metanol pada akar dan daun bakau merah menggunakan metode DPPH. Ekstraksi senyawa aktif pada akar dan daun dilakukan dengan metode maserasi bertingkat menggunakan n-heksana, etil asetat dan metanol. Ekstrak kasar dilakukan uji fitokimia dan aktivitas antioksidannya menggunakan metode DPPH (<em>1,1-difenil-2-pikrilhidrazil</em>). Hasil pengujian fitokimia dari ekstrak kasar akar menunjukkan adanya senyawa flavonoid, tanin, dan steroid/triterpenoid, sedangkan ekstrak kasar daun menunjukkan adanya alkaloid, flavonoid, tanin, saponin, dan steroid/triterpenoid. Nilai EC<sub>50</sub> (<em>Efficient Concentration</em>) ekstrak metanol daun dan akar berturut-turut sebesar 5,01 dan 2,55 ppm, sedangkan ekstrak etil-asetat daun dan akar berturut-turut sebesar 89,94 dan 8,51 ppm. Adapun ekstrak n-heksana daun menghasilkan nilai EC<sub>50</sub> sebesar 33,14 ppm. Ekstrak akar dan daun bakau merah memiliki aktivitas antioksidan yang tinggi.</p><p><strong> </strong></p><p>Kata kunci: Antioksidan, bakau merah, metode DPPH</p>

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