scholarly journals PREVALENCE AND HAEMATOLOGICAL INDICES OF GARDIASIS AND MALARIA AMONGST PUPILS OF PAEDIATRIC AGE (0-15) IN OWERRI WEST IMO STATE, NIGERIA

2021 ◽  
Vol 9 (11) ◽  
pp. 595-601
Author(s):  
Nwachukwu J.C ◽  
◽  
Ukwandu N.C.D ◽  
Keyword(s):  
Primary Schools ◽  
Malaria Parasite ◽  
Haematological Parameters ◽  
Clear Correlation ◽  
Preparation Technique ◽  
Protozoan Parasites ◽  
Parasite Count ◽  
Imo State ◽  
Significant Difference ◽  
Paediatric Age

This study investigated the prevalence and haematological parameters of gardiasis and malaria amongst pupils of paediatric age (0-15)in 20 randomly selected primary schools was carried out in Owerri West Local Government Area of Imo State, Nigeria. Observations were made of the age, sex, socio-economic status of parents and the schools locations to determine the secondary outcomes measures to these two diseases. Both blood and faecal samples were collected from each of the pupils between the hours of 8.00 am and 11.00 am using 19cc needle guage and 10 ml syringes into EDTA bottles for the blood and sterile applicator sticks employed for the faeces, into wide-mouthed screwed, non-greasy universal bottles. Exclusion procedures were also carried out to eliminate other possible protozoal parasites including administration of combantrin tablets to the 150 volunteers for deworming. Blood parasitaemia due to malaria parasites was determined using the giemsa stained thick and thin smears, while giardiasis was determined using the faecal wet preparation technique involving the Lugols iodine faecal smears for the presence of trophozoites, cysts or casts. Determinations of other haematological parameters were carried out using histochemical techniques. Results showed out of 150 samples, 78(52.0%) were infected with either giardiasis, 20(25.64%) or other malaria parasite forms 30(38.46%), while 28(35.89%) had mixed infections of both malaria and giardiasis. A total parasite count for both infections was 1958 represented as 37% and 62.92% for giardiasis and malaria respectively. Forty-two (42) samples were discarded due to indeterminate results. RBC counts (X1012/L) for control: scarification (5.6±2.0), unscarification (2.7±0.3) and for test (infected): malaria (4.1±3.2), giardiasis (2.9±1.3), and malaria+giardiasis (1.4±0.02) showed marked significant difference (t=3.7, p<0.002) and similarly between both infection categories (t=1.8, p<0.007). Recorded values for PCV showed control: scarification (42.5±3.3), unscarification (26.2±2.2) and for test (infected): malaria (30.4±4.2), giardiasis (28.4±1.0) are indirectly proportional to intensity of infection establishing significant correlation between parasite count (PC) and PCV (r=0.45, p<0.002), PC and RBC (r=0.36, p<0.002), PC and reticulocyte (r=0.08, p<0.02). MCV values did not show clear correlation pattern with PC (r= -0.07, p>0.02). Mean proteins from the test (infected) groups: total protein (malaria, 6.13±0.01 & giardiasis, 4.50±0.10) albumin – (malaria, 5.52±0.30 & giardiasis, 7.41±2.2) globulin- (malaria, 6.40±0.15 & giardiasis, 8.15±0.22) reduced significantly (p<0.01) compared with each of the control groups and showed significant correlation (r=0.01, p<0.003) with PC: malaria (104.4±3.3x105), and giardiasis (76.2±5.8x105).Giardia lamblia and malaria parasite species are naturally prevalent protozoan parasites. Notable significant difference in prevalence of these protozoan parasites infestations among children abound.

Effect of washing procedures on trace-element content of hair.

1977 ◽  
Vol 23 (9) ◽  
pp. 1771-1772 ◽  
Author(s):  
G S Assarian ◽  
D Oberleas
Keyword(s):  
Trace Element ◽  
Trace Element Analysis ◽  
Trace Element Content ◽  
Magnesium Content ◽  
Preparation Technique ◽  
Element Analysis ◽  
Significant Difference ◽  
Trace Element Status ◽  
Mg Content ◽  
Source Of Information

Abstract A pooled sample of hair was divided and portions prepared for analysis by three washing procedures, to evaluate the effect of washing procedure on the subsequent trace-element (Zn, Cu, Mg) content. The methods selected were a detergent wash, a hexane-ethanol wash, and an acetone-ether-detergent wash. For all elements, there was a significant difference among the results after these wash procedures. Magnesium content of hair was most affected by washing, containing less than half of the magnesium of the unwashed hair. The detergent wash removed the most zinc and magnesium; the acetone-ether-detergent wash removed the most copper. Our results indicate that the trace-element analysis of hair is sensitive to the preparation technique and therefore is an unreliable source of information about trace-element status.

scholarly journals Inter-rater Variability in Malaria Microscopy at the LEKMA Hospital, Ghana

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Andrew Nii Adzei Bekoe ◽  
Emmanuel Alote Allotey ◽  
Elliot Elikplim Akorsu ◽  
Albert Abaka-Yawson ◽  
Samuel Adusei ◽  
...  
Keyword(s):  
Sub Saharan Africa ◽  
Medical Laboratory ◽  
Rater Agreement ◽  
Laboratory Personnel ◽  
Parasite Count ◽  
Laboratory Staff ◽  
Significant Difference ◽  
Expected Outcome ◽  
Sub Saharan ◽  
Group B

Background. Malaria remains a major cause of morbidity and mortality worldwide and particularly in sub-Saharan Africa where it is endemic. As such, it is important that a proper diagnosis is made before treatment is initiated. Malaria parasite count plays a key role in the diagnosis and management of malaria. Variations in ratings by laboratory personnel can impact negatively on the treatment regimen for malaria-infected patients. The study is thus aimed at evaluating and comparing the proficiency and parasitaemia counts by two different categories of laboratory staff at the LEKMA Hospital, Ghana. Materials and Methods. A total of 200 confirmed malaria-positive samples were used in the study. Six thick and thin films were prepared from each sample and uniquely labelled. Two of the six slides were given to two WHO-accredited malaria microscopists to examine and report their respective parasite count/μl ( parasite   count / WBC × 8000 ). These were used as the reference for the two categories of laboratory staffs: rater A being diploma holders (Technical Officers referred to as untrained rater) and rater B being degree holders (Medical Laboratory Scientist referred to as trained rater) at the LEKMA Hospital. Results. In comparison to the expected outcome, the parasite count by the rater group A (190 (151-239)]) and the rater group B (177 (140-224)) demonstrated significant positive correlation ( r = 0.995 , p < 0.0001 vs. r = 0.995 , p < 0.0001 , respectively) with the expected outcome in the cases of heavy parasitaemia. A statistically significant difference ( p < 0.05 ) between counts by the different raters in low parasitemia was observed in this study. A persistent nosedive inter-rater agreement from k = 0.82 to k = 0.40 with increasing density cutoff was observed in this study. Conclusion. The study observed that the degree of inter-rater agreement of parasite density count by various categories of laboratory personnel is almost perfect. However, the parasite count between raters varied significantly with very low levels of parasitemia but better correlated with heavy parasitemia.

scholarly journals Haematological and biochemical profiles of Gallus indigenous, exotic and hybrid chicken breeds (Gallus domesticus l.) from Rajshahi, Bangladesh

2015 ◽  
Vol 41 (2) ◽  
pp. 135-144 ◽  
Author(s):  
Ripon Kumar Dutta ◽  
M Saiful Islam ◽  
Md Ashraful Kabir
Keyword(s):  
Rhode Island ◽  
Biochemical Parameters ◽  
Gallus Domesticus ◽  
Haematological Parameters ◽  
Breeding Strategies ◽  
Significant Difference ◽  
Haematological Profile ◽  
Biochemical Profiles ◽  
Chicken Breeds ◽  
Differential Counts

A comparative account of haemato-biochemical profiles of an indigenus (IND) and five chicken breeds: viz. Cobb 500 (COB), cockerel (COC), Fayoumi (FAY), Rhode Island Red (RIR), and Sonali (SON, derived from RIR cock × FAY hen), has been presented. The haematological profile included total counts (TC) of RBC, WBC, platelets, haemoglobin (Hb%) and ESR and differential counts (DC) of WBC included neutrophils, eosinophils, lymphocytes, monocytes and basophils. All the haematological parameters except WBC, ESR and basophils showed significant difference among the breeds (P < 0.01). Vital biochemical parameters from blood sera such as calcium, cholesterol, creatinine, glucose and urea also showed significant difference among the chicken breeds (P < 0.05). The haematobiochemical parameters, however, were not significantly correlated (P > 0.05) with each other among the chicken breeds. Except for cholesterol vs. urea in IND, none of the correlations tested for haemato-biochemical profiles of the experimental chickens was found significant. Relevance of this study in relation to health, clinico-pathology and improved breeding strategies of the poultry species in the country has been discussed.Bangladesh J. Zool. 41(2): 135-144, 2013

scholarly journals To what extent are GCS and AVPU equivalent to each other when assessing the level of consciousness of children with head injury? A cross-sectional study of UK hospital admissions.

BMJ Open ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. e023216 ◽  
Author(s):  
Amy GL Nuttall ◽  
Katie M Paton ◽  
Alison M Kemp
Keyword(s):  
Head Injury ◽  
Hospital Admissions ◽  
Diagnostic Yield ◽  
Cross Sectional Study ◽  
Initial Assessment ◽  
Clear Correlation ◽  
Sectional Study ◽  
Cross Sectional ◽  
Significant Difference ◽  
Level Of Consciousness

ObjectiveTo evaluate utility and equivalence of Glasgow Coma Scale (GCS) and the Alert, Voice, Pain, Unresponsive (AVPU) scale in children with head injury.DesignCross sectional study.SettingUK hospital admissions: September 2009–February 2010.Patients<15 years with head injury.InterventionsGCS and/or AVPU at injury scene and in emergency departments (ED).Main outcomeMeasures used, the equivalence of AVPU to GCS, GCS at the scene predicting GCS in ED, CT results by age, hospital type.ResultsLevel of consciousness was recorded in 91% (5168/5700) in ED (43%: GCS/30.5%: GCS+AVPU/17.3%: AVPU) and 66.1% (1190/1801) prehospital (33%: GCS/26%GCS+AVPU/7%: AVPU). Failure to record level of consciousness and the use of AVPU were greatest for infants. Correlation between AVPU and median GCS in 1147 children <5 years: A=15, V=14, P=8, U=3, for 1163 children ≥5 years: A=15, V=13, P=11, U=3. There was no significant difference in the proportion of infants who had a CT whether AVPU=V/P/U or GCS<15. However diagnostic yield of intracranial injury or depressed fracture was significantly greater for V/P/U than GCS<15 :7/7: 100% (95% CI 64.6% to 100%) versus 5/17: 29.4% (95% CI 13.3% to 53.1%). For children >1 year significantly more had a CT scan when GCS<14 was recorded than ‘V/P/U only’ and the diagnostic yield was greater. Prehospital GCS and GCS in the ED were the same for 77.4% (705/911).ConclusionThere was a clear correlation between Alert and GCS=15 and between Unresponsive and GCS=3 but a wider range of GCS scores for responsive to Pain or Voice that varied with age. AVPU was valuable at initial assessment of infants and did not adversely affect the proportion of infants who had head CT or the diagnostic yield.

scholarly journals Prevalence of Soil Transmitted Helminthes (STHs)Among Pupils of Community Primary Schools in Nkpor and Mgbodohia, Obio/Akpor Local Government Area, Rivers State, Nigeria

2018 ◽  
Author(s):  
LeBari Barine Gboeloh ◽  
Ike-Ihunwo Chikaire Ndamzi
Keyword(s):  
Public Health ◽  
Local Government ◽  
Mixed Infection ◽  
Primary Schools ◽  
Local Government Area ◽  
Sub Saharan Africa ◽  
Health Concern ◽  
Public Health Concern ◽  
Significant Difference ◽  
Rivers State

ABSTRACTSoil transmitted helminthes (STHs) are common public health concern among children in Sub saharan Africa. A study to determine the prevalence and intensity of these parasites among pupils of two primary schools in Nkpor and Mgbodohia communities, Obio/Akpor Local Government Area, Rivers State, Nigeria was conducted. The formo-ether concentration technique was used to concentrate and separate the eggs and cysts from the faeces. Out of 107 pupils (56males and 51 females) investigated, 81 (75.7 %) were positive for at least one gastrointestinal helminth. The parasites identified included Ascaris lumbricoide(58.0%), Hookworms(28.4%), Trichirus trichiura(13.6%) and A.lumbricoide + T. trichiura (6.3%). Ascaris lumbricoide was significantly (P<0.05) higher in prevalence than other parasites. Although more females (54.3%) were infected than males (45.7%), there was no significance(P>0.05) difference in the prevalence in relation to sex. Of the 44 males infected, 24(54.5%), 5 (11.4%), 13(29.5%) and 2(4.5%) haboured A. lumbricoide, T. trichiura, Hookworms and mixed infection (A. lumbricoide + T. trichiura) repectively. Out of the 37 females infected, 21 (56.8%), 3(8.1%), 10(27.0%) and 3(8.1%) haboured A. lumbricoide, T. trichiura, Hookworms and mixed infection (A. lumbricoide + T. trichiura) repectively. There was no significance difference in the prevalence of A. lumbricoide and Hookworms between males (54.5%) and females (56.8%). There was significant difference in prevalence among two major age groups (5-10years-45% and 11-15years-41.9%). Children within the age group of 16-20years had the least infection (9.9%). The intensities of A.lumbricoide, T.trichiura and hookworm were 246.5, 107.5 and 187 Epg respectively. The intensity of A. lumbricoide was significantly difference than other parasites identified.Soil transmited helminthes remain a public health concern among children in the study area. Provision of portable water, toilet facilities, good education on the epidemiology of STHs and regular de-worming will enhance control measures.

scholarly journals IMPACT OF MODELLING TECHNOLOGY INTEGRATION FOR OUT-OF-SCHOOL TIME LEARNING ON ACADEMIC ACHIEVEMENT

2020 ◽  
pp. 292-299
Author(s):  
Dr. Thadei A. Kiwango
Keyword(s):  
Academic Achievement ◽  
Technology Integration ◽  
Primary School ◽  
Primary Schools ◽  
Significant Difference ◽  
The Government ◽  
Arusha Region ◽  
Primary School Pupils ◽  
The Impact ◽  
Out Of School

This paper determines the impact of modelling technology integration for of out-of-school (OST) learning on academic achievement in primary schools. The research was conducted in Arusha Region, specifically in Meru District. The paper adopted the experimental design, involving experimental and control groups. Each of the two groups comprised three (3) schools, making a total of six (6) schools. The experiment was conducted using Digital Video Disks (DVDs), mobile phones, notebooks and home assignment booklets. The tools were further supplemented by a list of perceived best practices, and examination papers. The findings reveal a statistically significant difference in mean scores between the control and experimental groups as confirmed by 95% confidence level whereby, F (1, 180) = 28.63, p=0. Based on the findings, null hypothesis was rejected, leading to the conclusion that the proposed model for OST technology integration is attributed to significant improvements in academic achievement for primary school OST learners. The implication of these findings is for researchers, and other educational stakeholders, including the government to invest in devising contextually relevant model, and mobilizing parents, teachers and learners with a view to hastening technology integration in order to improve academic achievements for primary school pupils. There is also a need for studies that further explore technology integration opportunities, and associated challenges in a bid to addressing poor academic performance among primary school pupils.

scholarly journals Setting expectations: Building the blocks of phonemic awareness

2021 ◽  
Author(s):  
W. L. Quint Oga-Baldwin ◽  
Kaori Nakao ◽  
Luke K. Fryer
Keyword(s):  
Elementary School ◽  
Phonemic Awareness ◽  
Primary Schools ◽  
School Students ◽  
English Classes ◽  
Instructional Context ◽  
Significant Difference ◽  
The Difference ◽  
Identity Test ◽  
English Instruction

Young learners require developmental benchmarks to improve awareness of the phonemes in a new language. This study aimed to extend our understanding of Japanese elementary school students’ general and specific phonemic awareness across four years of English instruction. A public elementary school 3rd-6th year students in Japan (n=261, ages=8–12) participated in this study. The phoneme-identity test used consisted of fifteen items employing words during students' regular English classes. The effect of year on achievement and the difference between grades was tested. There was a significant effect of students' year on test score (p&lt;.05). While there were no statistically significant difference years 4-5, and between years 5-6, students’ phonemic awareness increased two-year increments. Moreover, patterns of specific phoneme difficulties were identified. The present study provides preliminary guidelines for understanding the intersection between first and foreign languages, instructional context and their shared contribution towards listening and reading development in primary schools.

scholarly journals OVERVIEW OF MALARIA PARASITE AND ITS PREVENTION IN INDIA

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Adil Raza ◽  
Megha Chaudhary ◽  
Sonika Devi
Keyword(s):  
Red Blood Cells ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Malaria Parasite ◽  
Plasmodium Species ◽  
Peripheral Blood Smear ◽  
Infected Mosquito ◽  
Protozoan Parasites ◽  
Malaria Parasites ◽  
Genus Plasmodium

Background: Malaria is a systematic disease caused by a parasite called Plasmodium which is transmitted into the human blood via female Anopheles mosquito. Malaria in humans is caused by four species of protozoan parasites of the genus Plasmodium: P. falciparum, P. vivax, P. ovale, and P. malariae. The parasite enters the human body through a mosquito bite and travel to the very crucial organ, the liver, where they multiply and come back to the bloodstream and destroy red blood cells. Malaria causes symptoms that typically include fever, tiredness, vomiting, and headaches. In severe cases it can cause yellow skin, seizures, coma, or death. Symptoms usually begin ten to fifteen days after being bitten by an infected mosquito. In those who have recently survived an infection, reinfection usually causes milder symptoms. Objectives: Isolation of different species of malaria parasites. The prevalence of malaria parasite in India. Methods: The procedure follows these steps: collection of peripheral blood, staining of smear with Leishman’s stain and examination of red blood cells for malaria parasites under the microscope. Results: We observed the plasmodium species in peripheral blood smear. Conclusion: Worldwide, the number of cases of malaria caused by Plasmodium falciparum, the most dangerous species of the parasite, is on the rise.

scholarly journals Gender as a Factor in Predicting Academic Performance in Agriculture in Primary School Leaving Examination in Gaborone, Botswana

2019 ◽  
pp. 1-8
Author(s):  
Tapela Bulala ◽  
Keabetswe Malima
Keyword(s):  
Academic Performance ◽  
Primary School ◽  
Parental Support ◽  
Primary Schools ◽  
Economic Status ◽  
Government Schools ◽  
Significance Level ◽  
Ex Post ◽  
Significant Difference ◽  
Males And Females

The study looked at gender as it relates to academic performance of students in Agriculture Primary School Leaving Examination in Gaborone schools. The research was quantitative ex-post facto type design. The sample composed of 3781 (1751 males and 2030 females) students who wrote PSLE in 2012. The sample was taken from 43 primary schools in Gaborone area which included private and government schools. The results which included students’ scores were obtained from Botswana Examinations Council academic records and were not manipulated in any way. The results were then subjected to testing using Statistical Package for Social Sciences at significance level of 0.05. An independent t-test was conducted to determine significance difference and value of -5.964 which is statistically significance (P = 0.00) obtained and therefore the null hypothesis that states that there is no significant difference between males and females was rejected. The analysis revealed that girls performed better than boys and moreover there is a significant difference between males and females’ academic performance. It was therefore, recommended that the Gaborone Regional Education office intensify research to establish how other variables such as location, parental support and economic status of parents contribute to poor Primary School leaving Examinations.

scholarly journals Comparison of the Change in the Prevalence and Intensity of Schistosoma haematobium Infection Between High and Low Prevalence Areas of White Nile State, Sudan

2020 ◽  
Vol 58 (4) ◽  
pp. 421-430 ◽  
Author(s):  
Seungman Cha ◽  
Sung-Tae Hong ◽  
Jin-Su Lee ◽  
Hoo Gn Jeong ◽  
In-Sun Kwon ◽  
...  
Keyword(s):  
Primary Schools ◽  
Schistosoma Haematobium ◽  
Geometric Mean ◽  
Infection Intensity ◽  
Baseline Survey ◽  
Relative Reduction ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Significant Difference ◽  
White Nile

This study aimed to investigate whether mass drug administration (MDA) intervention has an equivalent effect on reducing the prevalence and intensity of Schistosoma haematobium infection regardless of the baseline values. A repeated cross-sectional survey was performed targeting students of 12 primary schools in Al Jabalain and El Salam districts of White Nile State, Sudan, at both 1 week before and 8 months after the MDA. Prior to the baseline survey, school-aged children in Al Jabalain had received MDA interventions twice in 4 years, while those in El Salam had not. The baseline prevalence was 9.1% in Al Jabalain and 35.2% in El Salam, which were reduced to 1.8% and 5.5% at 8 months after the MDA, respectively. The corresponding reduction rates were 80.3% and 84.4%, not significant difference between both districts. However, changes in the geometric mean intensity (GMI) of egg counts were significantly different between both districts. The baseline GMIs were 14.5 eggs per 10 ml of urine (EP10) in Al Jabalain and 18.5 EP10 in El Salam, which were reduced to 7.1 and 11.2 EP10 after treatment, respectively. The corresponding reduction rates were 51.0% and 39.5%. In conclusion, MDA interventions were found to bring about similar relative reduction in prevalence regardless of the baseline value; however, the relative reduction in infection intensity was more salient in the district with a low baseline value for both prevalence and intensity. This clearly points to the importance of repeated MDA interventions in endemic areas, which will eventually contribute to schistosomiasis elimination.

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