Evaluation of ethanol preconditioning as a rapid seed vigour and viability test

It is essential that tests for evaluating seed vigour be faster and increasingly efficient to enable precise differentiation among batches. In this way, it is possible to evaluate the quality of seeds based on the anaerobic metabolism of cells when exposed to environments lacking oxygen. Thus, the objective of this study was to establish methodology for evaluating the viability and vigour of 3 lots of cowpea (Amendoim cultivar) seeds using the ethanol test. The treatments were carried out in a completely randomized design with four replications. For the test, 25 seeds were stored in hermetically sealed PET (Polyethylene the Ethylene) bottles containing 40 ml of distilled water and subjected to 3 soaking times in distilled water (6, 24, and 48 h) at a controlled temperature of 40°C in a germinator. The amount of ethanol produced was quantified with the aid of an adapted breath analyser. The results are expressed as mg L-1; these data were then compared with data for the following: germination; the first germination count; the total length, root length and shoot length of the seedling; dry weight of the seedling; emergence in the field; emergence speed index; and electrical conductivity. The experimental design was completely randomised, and the data were subjected to analysis of variance and correlation analysis. The results were compared using the Tukey test at 5% probability. Measurement of ethanol after 6 or 48 h of soaking at 40°C was effective for determining the viability and vigour of cowpea seeds. As ethanol test results have high correlation with germination and vigour test results, this approach is a viable alternative for analysts and seed producers.

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Effect of Seed Rate on Seedling Quality for Mechanical Rice Transplanting

Bangladesh Rice Journal ◽

10.3329/brj.v22i1.41834 ◽

2019 ◽

Vol 22 (1) ◽

pp. 9-23

Author(s):

MA Hossen ◽

MM Hossain ◽

ME Haque ◽

RW Bell

Keyword(s):

Rice Variety ◽

Seedling Emergence ◽

Dry Weight ◽

Seed Rate ◽

Shoot Dry Weight ◽

Root Shoot Ratio ◽

Randomized Design ◽

Seedling Length ◽

Main Factor

A study was conducted in the Farm Machinery and Postharvest Technology Division, Bangladesh Rice Research Institute (BRRI), Gazipur in the irrigated dry season (Boro) of 2012-13. It was aimed at identifying the optimum seed rate of different graded rice variety for mat type seedlings preparation and suitable seedling adjustment option of the rice trans planter to maintain required number of seedlings dispensed per stroke by the rotary picker of the trans planter. Walk behind type 4-rows rice trans planter (DP 480) was used to conduct the study. The experiment was followed as two factorial completely randomized design (CRD) with three replications. Three categories of rice variety as short and bold grain (cv. BR3), medium and slender grain (cv. BRRI dhan28) and extra-long and slender grain (cv. BRRI dhan50) were considered as main factor whereas seed rate of 100, 120, 130, 140, 150 and 160g pertray (280×580×25 mm) were considered as sub-factor. Irrespective of rice category, seedling emergence decreased with the increase of seed rate. Seedling emergence decreased from 77 to 56, 74 to 57 and 77 to 54% in BR3, BRRI dhan28 and BRRI dhan29 respectively with the increase of seed rate from 100 to 160g per tray. Seed rate did not affect the seedling height significantly whereas it was varied with the rice verities only and BRRI dhan50 produced higher seedling length. However, number of leaf, stem thickness and shoot dry weight decreased and root-shoot ratio increased significantly with the increase of seed rate. The highest shoot dry weight was observed in BR3 followed by BRRI dhan50. Seedling strength also decreased with the increase of seed rate. The highest seedling strength (0.043 mg cm-1) was observed for the seed rate of 100g of BR3 and the lowest (0.020 mg cm-1) for the seed rate of 160g of BRRI dhan28. The number of seedling increased and percentage of missing hills decreased with the increase of both the seed rate and seedling adjustment option of the rice transplanter irrespective of variety. In case of BR3, seedling dispensed per stroke and percentage of missing hills varied from 4.3 to 5.7 and 6.8 to 7.8 for the seed rates of 140, 150 and 160 g of seeds tray-1 for 5 to 7 seedling adjustment option of the rice transplanter respectively. Seedlings per stroke and percentage of missing hills of BRRI dhan28 for the seed rate of 130, 140 and 150 g of seeds tray-1 was found almost same for 5 to 7 seedling adjustment options of the rice transplanter (4.2 to 6.3 and 5.7 to 9.8). However, there was minimum difference of seedlings per stroke and percentage of missing hills among 120, 130 and 140 g of seeds tray-1 for the option of 4 to 7 (4.0 to 5.6 and 3.9 to 7.8 respectively) for BRRI dhan50. It can be concluded that 140 g of seeds tray-1 for short and bold grain (BR3) and 130 g of seeds tray-1 for medium and slender grain (BRRI dhan28) under the seedling adjustment options of 5 to 7 and 120 g of seeds tray-1 for extra-long and slender grain (BRRI dhan50) under the seedling adjustment options of 4 to 7 may be used for desired seedlings per hill and minimum missing hills along with good quality of seedling. Bangladesh Rice j. 2018, 22(1): 9-23

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Shading and DCPTA Interactively Influence Shoot Growth of Hypoestes

HortScience ◽

10.21273/hortsci.30.4.854d ◽

1995 ◽

Vol 30 (4) ◽

pp. 854D-854

Author(s):

Sven E. Svenson

Keyword(s):

Shoot Growth ◽

Seedling Emergence ◽

Tween 80 ◽

Dry Weight ◽

Distilled Water ◽

Seedling Height ◽

Shoot Dry Weight ◽

Randomized Design ◽

Completely Randomized Design ◽

Shoot Weight

The objective of this study was to determine if shading and DCPTA application interactively influence seedling emergence and shoot growth of Hypoestes phyllostachya Bak. seedlings. Hypoestes `Carmine Red' seeds were soaked for 6 h in distilled water, and then soaked 6 h in solutions containing' 10 mg DCPTA/liter (30 mM) and 0.1% Tween-80, 0.1% Tween-80, or distilled water. Date of seedling emergence was recorded after sowing (0.5 cm) in 9-cm (460 ml) pots filled with 440 ml of a 5 pine bark: 4 Florida sedge peat: 1 sand medium. Forty pots from each of the three seed-soak treatments were grown under 30%, 63%, or 84% shading, provided by saran-type shadecloth, using natural photoperiods (completely randomized design). Shoot heights and dry weights were recorded 75 days after sowing. Neither shading nor DCPTA influenced total seedling emergence or seedling emergence rate (time to 50% emergence). Under 30% shading, seedlings from DCPTA-treated seeds were taller and had more shoot dry weight than seedlings from surfactant- or water-treated-seeds; however, DCPTA did not influence seedling height or shoot weight under 63% or 84% shading.

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Evaluation of seed physiological quality of papaya elite hybrids, their reciprocal crosses and parents

Journal of Seed Science ◽

10.1590/s2317-15372013000200008 ◽

2013 ◽

Vol 35 (2) ◽

pp. 190-197 ◽

Cited By ~ 5

Author(s):

Célia Maria Peixoto de Macedo ◽

Messias Gonzaga Pereira ◽

Deisy Lúcia Cardoso ◽

Roberto Ferreira da Silva

Keyword(s):

Seed Quality ◽

Seedling Emergence ◽

Dry Weight ◽

Fresh Weight ◽

Reciprocal Effect ◽

F1 Hybrid ◽

Reciprocal Crosses ◽

Randomized Design ◽

Physiological Quality

This study aimed to evaluate the physiological quality of seeds of three hybrid combinations and their reciprocals, as well as the selfed parents, and to estimate genetic parameters associated with seed quality, and possible reciprocal effects. The experiment used F1 hybrid papaya seeds and their reciprocal crosses between JS12 and Sunrise Solo 72/12, Sekati and Waimanalo, and the seeds of the parents and two varieties, totaling 12 treatments in a completely randomized design with four replications. Weight of 1,000 seeds, germination, seedling fresh weight and seedling dry weight, first count, speed of germination and seedling emergence were determined. There was no xenia effect for weight of 1,000 seeds and seedling fresh weight. The reciprocal effect was not expressed for germination and seedling emergence, while for the other traits, it was not expressed only for JS12 x Sekati. Differences among hybrids and their parents occurred as a result of the action of pollen in the crosses, and justified by early heterosis. The most sensitive vigor tests to discriminate differences in performance of hybrids and their reciprocals were speed of germination and seedling fresh weight. There were high magnitudes of the estimates for the genotypic determination coefficient for most of the evaluated variables, except for seedling fresh weight.

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The Effect of Acetic Acid Treatments on the Quality of Stored Carrot (Daucus carota L.) Seeds

Agronomy ◽

10.3390/agronomy11061176 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1176

Author(s):

Hanna Dorna ◽

Agnieszka Rosińska ◽

Dorota Szopińska

Keyword(s):

Acetic Acid ◽

Distilled Water ◽

Storage Duration ◽

Seed Vigour ◽

Daucus Carota L ◽

Two Samples ◽

Before And After ◽

Alternaria Dauci ◽

Acid Treatments

Alternaria dauci (J.G. Kühn) J.W. Groves et Skolko and A. radicina Meier, Drechsler et E.D. Eddy are important seed-transmitted pathogens of carrot. The aim of this study was to evaluate the effects of acetic acid treatments on the quality of stored carrot seeds. Seeds of two samples were soaked for 30 min in 0.5, 1 and 2% acetic acid. Controls included untreated seeds, seeds soaked in distilled water and seeds treated with fungicide Zaprawa Nasienna T 75 WS/DS (a.i. thiram 75%). Germination, vigour and health of untreated and treated seeds were evaluated before and after 5 and 12 months of storage at 4 and 20 °C. Seeds of both samples treated with 0.5 and 1% acetic acid were characterized by higher germination capacity after storage than untreated seeds. However, treatments with 1 and 2% acetic acid negatively affected seed vigour. Generally, seeds of both samples treated with acetic acid were characterized by lower infestation with A. alternata and A. radicina after storage than untreated seeds and seeds soaked in distilled water. Moreover, acetic acid often controlled these fungi more effectively than the fungicide. Regardless of the storage duration, infestation with fungi was higher if seeds of both samples were stored at a lower temperature.

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Application of Trichoderma harzianum on the physiological quality of cowpea seeds

Australian Journal of Crop Science ◽

10.21475/ajcs.21.15.05.p2865 ◽

2021 ◽

pp. 643-648

Author(s):

Marcelo Laranjeira Pimentel ◽

Henara Valéria Miranda Castro ◽

Mike Kirixi Munduruku ◽

Larissa Conceição Cunha Ponte ◽

Deyvielen Maria Ramos Alves ◽

...

Keyword(s):

Trichoderma Harzianum ◽

Seed Treatment ◽

Germination Percentage ◽

Hypocotyl Length ◽

Cowpea Seeds ◽

Randomized Design ◽

Germination Speed ◽

Physiological Quality ◽

Set Up

The use of fungi in seed treatment can improve the plant's physiological characteristics. The aim of this study was to evaluate the influence of the use of Trichoderma harzianum on the physiological quality of bean seeds. The experiment was set up and conducted in the forest seed laboratory of the Federal University of Western Para, in a randomized design, using 4 procedures on the basis of a colony forming unit (CFU): T1 (0 CFU), T2 (4 x 109 CFU), T3 (8 x 109 CFU) and T4 (12 x 109 CFU), with 5 repetitions. Germination percentage (G%), germination speed index (GSI), hypocotyl length (HL) and radicle length (RL) were evaluated as variables. The data were analyzed by variance analysis and the averages were compared using the Tukey test (p≤0.05), in addition to the regression analysis, using the Minitab© version 18 statistical software. There were differences between the germination percentage tests, in which T2 obtained 100% of germinated seeds, for GSI it was observed that T2 and T3 were equal and superior to the other treatments, while for RL T1 and T2 obtained the best results, however, for HL there were no statistical differences between treatments. T2 was more efficient in the physiological quality of seeds to germination percentage and germination speed index

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Exogenous Antioxidants Enhance Seedling Growth and Yield of Artificially Aged Cabbage and Lettuce Seeds

Horticulturae ◽

10.3390/horticulturae7090274 ◽

2021 ◽

Vol 7 (9) ◽

pp. 274

Author(s):

Ademola Adetunji ◽

Sershen ◽

Boby Varghese ◽

Norman Pammenter

Keyword(s):

Leaf Area ◽

Seedling Growth ◽

Seedling Emergence ◽

Dry Weight ◽

Growth And Yield ◽

Seed Vigour ◽

Beneficial Effects ◽

Shoot Dry Weight ◽

Antioxidant Defence System ◽

Aged Seeds

Aged seeds exhibit compromised vigour in terms of germination, seedling emergence and growth, but this can to some extent be alleviated by invigoration treatments before sowing. This study aimed to investigate ageing rates and patterns in cabbage (Brassica oleraceae) and lettuce (Lactuca sativa) seeds and whether the beneficial effects of invigorating aged seeds with exogenous antioxidants translate to enhanced seedling performance. Seeds were artificially aged to 25% viability before soaking in 0.4 mM glycerol, 0.6 mM GSH and 0.2 mM trolox for cabbage, and 0.6 mM glycerol, GSH and trolox for lettuce; deionised water served as a control. After 14 days of sowing, seedling emergence percentage, mean emergence time, mean daily emergence, and time taken to 25% emergence were computed. Seedling vigour index, root and shoot dry weight, root:shoot ratio, leaf area, leaf area ratio, and leaf chlorophyll content were assessed 6 weeks after sowing. Furthermore, the photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (E), and chlorophyll fluorescence were measured 6 weeks after sowing. Notably, ageing resulted in the loss of seed vigour and viability at higher rates in lettuce than cabbage. Seed pretreatment with glycerol promoted seedling growth in both species and shoot dry weight in lettuce, while glycerol and GSH enhanced Pn, Gs and E in lettuce. Trolox also enhanced Pn and E in lettuce. The beneficial effects of the antioxidant treatments are thought to be associated with the protection of photosystems from oxidative stress and/or stimulation of enzymes involved in photosynthesis, possibly through an enhanced antioxidant defence system during the early development stages when seedlings are particularly vulnerable to stress.

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KUALITAS MINUMAN SARI BIJI NANGKA (Artocarpus heterophyllus)DENGAN PENAMBAHAN JAHE MERAH(Zingiber officinale varrubrum rhizoma)

Jurnal Pendidikan Teknologi Pertanian ◽

10.26858/jptp.v5i0.8566 ◽

2019 ◽

Vol 5 ◽

pp. 115

Author(s):

Syahri Fadhila Al-Farid ◽

Jamaluddin Jamaluddin ◽

Andi Sukainah

Keyword(s):

Protein Content ◽

Water Content ◽

Zingiber Officinale ◽

Carbohydrate Content ◽

Test Results ◽

Artocarpus Heterophyllus ◽

Randomized Design ◽

Completely Randomized Design ◽

Red Ginger

This study aims to determine the quality of jackfruit seed juice drinks with the addition of red ginger and to determine the level of acceptance of panelists on jackfruit seed juice drinks. Parameters observed included water content, protein content, carbohydrate content and organoleptic including color, taste, aroma and texture. This study used a Completely Randomized Design (CRD) consisting of one factor, namely the concentration of red ginger juice with four treatments, namely: 0%, 6%, 12%, 18%, each repeated three times. The results showed that jackfruit seed juice drink with the addition of 18% red ginger was the best treatment from the results of organoleptic tests. The proximate test results showed that the best treatment was 3.13% water content in 12% ginger treatment, 2.17% protein in 18% red ginger treatment and 95.61 carbohydrate in 18% red ginger treatment.

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EKSTRAK KARAGINAN RUMPUT LAUT (Eucheuma cottonii) YANG DIPEROLEH DARI PANTAI TABLOLONG

Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi ◽

10.33323/indigenous.v3i3.137 ◽

2021 ◽

Vol 3 (3) ◽

pp. 119-127

Author(s):

Runa Matias Ruku ◽

Alfred G.O Kase ◽

Hartini R.L Solle

Keyword(s):

Fat Content ◽

Ash Content ◽

Test Results ◽

A Value ◽

Randomized Design ◽

Yield Test ◽

Completely Randomized Design ◽

Quantitative Descriptive ◽

Treatment Concentration

ABSTRACTEucheuma cottonii (E. cottonii) seaweed is a low level plant that has a high nutritional value. One of the ingredients that play a role in the formation of texture is carrageenan.Carrageenan is a type of hydrocolloid extracted from the seaweed of the red algae group (Rhodophyceae). The purpose of this study was to determine the quality of yields of Seaweed Carrageenan Extract (E. cottonii) obtained from Tablolong beach in the form of yield, ash content and fat content. The method used in this study is an experimental method with a completely randomized design (CRD) and for fat testing using quantitative descriptive methods. This study uses various concentrations of 0.1 N KOH, 0.2 N KOH, 0.3 N KOH and 0.4 N KOH. The results of the carrageenan yield test show that the addition of 0.1 N KOH concentration has a value of 56.10, KOH 0 , 2 N 62.16, KOH 0.3 N 72.73 and the best treatment was at P4 KOH 0.4 N 80.90. Carrageenan ash content test results showed that the addition of KOH concentration value obtained P1 KOH 0.1 N was 60.50, P2 KOH 0.2 N was 70.70, P3 KOH 0.3 N was 80.50, and P4 KOH 0.4 with a value of 90.50. The results of the carrageenan fat content of 0.1 KOH treatment has a fat content of 0, 2 while the treatment of KOH 0,2, KOH 0,3, KOH 0,4 have no fat content by showing the value (0). The results showed that the highest yield in the treatment of KOH concentration of 0.4 N with a value 80.95% and carrageenan ash content has the highest value in the treatment concentration of 0.4 N with a value of 90.50% while the fat content has a value of 0.2 in the treatment of KOH concentration of 0.1 N Keywords: Carrageenan, extract of E. cottonii seaweed.

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Physiological maturity and drying speed in the quality of zucchini ( Cucurbita pepo L.) seeds

Journal of Seed Science ◽

10.1590/2317-1545v39n2171033 ◽

2017 ◽

Vol 39 (2) ◽

pp. 142-149

Author(s):

Hellismar Wakson da Silva ◽

João Almir Oliveira ◽

Lucinda Helena Fragoso Monfort ◽

Jodson Moraes dos Santos ◽

Ana Clara Reis Trancoso ◽

...

Keyword(s):

Seed Quality ◽

Seedling Emergence ◽

Seed Mass ◽

Accelerated Aging ◽

Maturity Stage ◽

Physiological Maturity ◽

Randomized Design ◽

Maturation Stages ◽

Seed Health Test

Abstract: Harvesting and drying are essential procedures to obtain high quality seeds as well as for their conservation during storage. The goal of this research was to identify the physiological maturity stage and to verify the effect of drying speeds on the quality of zucchini seeds. A completely randomized design in a 3x2x2 factor scheme was used, involving three fruit maturation stages (49, 56 and 63 days after anthesis - DAA), two drying speeds (slow and quick) and two storage periods (0 and 6 months). Seed quality was evaluated by germination, first count, accelerated aging, electrical conductivity, tetrazolium, seedling emergence, emergence speed index and seed health test. Moisture content, thousand seed mass and percentage full seeds were also evaluated. Zucchini seeds reach maximum quality at 49 DAA. Slow or quick drying and storage do not affect the physiological quality of seeds that were harvested at 49 DAA. The delayed harvest of fruits reduces the quality of seeds. Quick drying reduces the incidence of the fungi Alternaria, Cladosporium, Phoma and Fusarium, and increases the occurrence of Aspergillus and Penicillium.

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Rooting of Herbaceous and Semihardwood Cuttings of Guava cv. Século XXI, under Varying Concentrations of Indolebutyric Acid

Journal of Experimental Agriculture International ◽

10.9734/jeai/2019/v33i130135 ◽

2019 ◽

pp. 1-9

Author(s):

Jussara Cristina Firmino Da Costa ◽

Rejane Maria Nunes Mendonça ◽

Gerciane Cabral Da Silva ◽

Silvanda de Melo Silva ◽

Walter Esfrain Pereira ◽

...

Keyword(s):

Experimental Design ◽

Significant Interaction ◽

Dry Weight ◽

Psidium Guajava ◽

Commercial Production ◽

Control Treatment ◽

Distilled Water ◽

Indolebutyric Acid ◽

Rooting Of Cuttings

In the commercial production of guava seedlings (Psidium guajava L.) the quality of the cuttings, homogeneity, high percentage of rooting are the factors important to be analyzed. Therefore, as the Século XXI cultivar is recent, it is necessary to do more studies regarding the behavior of this guava to the factors that aid in the rhizogenic process. The objective of this work was to verify the concentrations of indolebutyric acid (IBA) that provide the best rooting of cuttings herbaceous and semihardwood, as well as to verify the best kind of cuttings used for vegetative propagation the guava cuttings cultivar ‘Século XXI’. The experimental design was completely randomized in a 5 x 2 + 1 factorial design, beginning with five concentrations of IBA (0, 1000, 1500, 2000 and 2500 mg L-1), two kinds of cuttings (herbaceous and semihardwood) and one control treatment (immersed in distilled water for 12 hours), with four replications and 10 cuttings per plot. In relation of the variables: the percentage of cuttings live without roots, callus, number of roots and dry weight of shoots did not present significant interaction among treatments, not adjusting to any regression model. Therefore, concluding that the herbaceous cutting is the most indicated for the propagation of guava seedlings of ‘Século XXI’; The concentration of 2500 mg L-1 of IBA promotes a higher percentage of rooted semihardwood cuttings without leaf; The hormone solution diluted with alcohol 50% (v/v) resulted in toxicity for herbaceous and semihardwood cuttings cv. Século XXI.

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