The growth of the Ukrainian economy largely depends on the effective use of the intellectual, scientific and technological potential of the country, the rapid pace of commercialization of high-tech scientific developments and its implementation in key areas of the national economy. In the period 2018–2020, the State Scientific Organization “Ukrainian Institute of Scientific and Technical Expertise and Information” (hereinafter — UkrISTEI) implements the project “Development of the Interregional Technology Transfer Network” (hereinafter — Project), within the framework of which the Interregional Office for the Transfer of Knowledge and Technologies on the basis of UkrISTEI was created; two pilot regional centers for the transfer of knowledge and technology in Odesa and Kharkiv were created. This Project is aimed at the widespread introduction of modern technologies in the real sector of the economy — as one of the tasks on the agenda of not only Ukraine, but also the European Union. The article assesses the potential of pilot regions through research that unites enterprises, scientific institutions, organizations, universities and other legal entities, regardless of the form of ownership, which create or use technologies or objects of intellectual property rights or relate to innovation infrastructure. The activities within the framework of the project “Development of an interregional technology transfer network” (hereinafter referred to as the Project) to increase the competitiveness of regions and increase their innovative development were presented, the role of the Project in the innovative development of regions was highlighted. The article defines the role of the Interregional Office and regional centers as centers of technology transfer and innovation: also a number of measures to activate the startup movement in Kharkiv and Odesa were taken. The work within the framework of the project “Development of an interregional technology transfer network” allowed to improve the results of domestic scientific and technical developments introduction in the real sector of the economy and to give impetus to its dynamic growth, to raise the employees’ awareness level in offices, departments, etc. for the technologies transfer, developers, startups, etc.