Isolation, Characterization and Hepatoprotective Activity of Naturally Occurring Protopine against Simvastatin Induced Liver Toxicity in Experimental Rodents

2020 ◽  
Vol 16 (5) ◽  
pp. 568-575
Author(s):  
Ramesh K. Gupta ◽  
Sudhansu R. Swain ◽  
Jagannath Sahoo ◽  
Sachin Chaudhary ◽  
Amresh Gupta
Keyword(s):  
Protective Effect ◽  
Total Protein ◽  
Total Bilirubin ◽  
Liver Toxicity ◽  
Hepatoprotective Activity ◽  
The Body ◽  
Central Vein ◽  
Spectroscopic Techniques ◽  
Traditional Use

Background: The present research reported isolation, characterization of protopine from Hedyotis corymbosa and investigation of in-vivo hepatoprotective activity of protopine against simvastatin induced hepatotoxicity in experimental rodents. Methods: Protopine was isolated from H. coryambosa by column chromatography using chloroform: methanol: diethylamine (9:1:1) as the mobile phase and structural characterization was done by UV, FTIR, 1H-NMR and 13C-NMR and mass spectroscopy, followed by determination of in-vivo liver protective effect of protopine against simvastatin (20 mg/kg, p.o.), induced hepatotoxicity in experimental rodents. The liver protective activity was assessed by interpreting distinct biochemical parameters like SGOT, SGPT, cholesterol, urea, total bilirubin, total protein and albumin along with the haematological and histopathological studies. Results: The reports of spectroscopic techniques confirmed that the isolated compound is protopine, an isoquinoline alkaloid. The treatment with protopine significantly at (P<0.05-P<0.001) and dosedependently reversed simvastatin induced elevated level of SGOT, SGPT, cholesterol, urea, total bilirubin and restored the total protein and albumin level in rodents. Furthermore, protopine also signifies the blood parameters at a dose of 11 and 22 mg/kg and restored the defence mechanism of the body. The histological examination revealed that protopine at a dose of 22 mg/kg showed the regeneration of hepatocytes around central vein with near normal liver architecture. Conclusion: The results of the current study confirm the liver protective effect of protopine against simvastatin induced hepatotoxicity and therefore, scientifically support its traditional use.

scholarly journals Hepatoprotective and antioxidant activity of ethanolic leaves extract of Avicennia marina against alcohol-induced liver toxicity in rats

2019 ◽  
Vol 9 (4-A) ◽  
pp. 403-408
Author(s):  
K Vellimalai ◽  
G Dinesh Kumar ◽  
K Jayaseelan
Keyword(s):  
Total Protein ◽  
Total Bilirubin ◽  
Liver Enzymes ◽  
Liver Toxicity ◽  
Avicennia Marina ◽  
Hepatoprotective Activity ◽  
Modern Medicine ◽  
Albino Rats ◽  
Test Plant ◽  
Basic Source

Plants play an important role in the life of human, as the major source of food, as well as for the maintenance and improvement of health and for the elimination of the enemies since ages. Plants are the basic source of knowledge of modern medicine. The present study was conducted to evaluate the hepatoprotective activity of ethanolic leaves extract  of Avicennia marina are evaluated in alcohol induced hepatotoxicity in rats. Silymarin (100mg/kg) was given as reference standard. The ethanolic leaves extract  of Avicennia marina have shown very significant hepatoprotection against alcohol induced hepatotoxicity in albino rats in reducing SGOT, SGPT, Alkaline phosphatase (ALP) and GGT and levels of total bilirubin and total protein were investigated and showed an increase in alcohol induced rats when compared to control. The extracts of the test plant exhibited significant (p < 0.01) hepatoprotective activity against the alcohol induced liver models by improving liver function which was indicated by reduction in the levels of SGOT, SGPT, ALP, GGT, total bilirubin and total protein. Keywords: Avicennia marina, Hepatoprotective, Liver Enzymes, Silymarin

Protective effect of Indian honey on acetaminophen induced oxidative stress and liver toxicity in rat

Biologia ◽  
2009 ◽  
Vol 64 (6) ◽  
Author(s):  
Ayyavu Mahesh ◽  
Jabbith Shaheetha ◽  
Devarajan Thangadurai ◽  
Dowlathabad Muralidhara Rao
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Liver Damage ◽  
Total Bilirubin ◽  
Liver Toxicity ◽  
Total Bilirubin Level ◽  
Serum Levels ◽  
Hepatoprotective Activity ◽  
Natural Medicine ◽  
Enzyme Markers

AbstractThe present study was undertaken to investigate the protective effect of Indian honey on acetaminophen induced oxidative stress and liver damage in rat. Honey serves as a source of natural medicine, which is effective to reducing the risk of heart disease, liver toxicity and inflammatory processes. The hepatoprotective activity of the Indian honey was determined by assessing levels of Serum transaminases, ALP and total bilirubin. Finally, the effects of the test substances on the antioxidant enzymes of the liver were also studied by assessing changes in the level of reduced glutathione, glutathione peroxidase, catalase and superoxide dismutase. Serum transaminase, ALP and total bilirubin level were significantly elevated and the antioxidant status in liver such as activities of SOD, CAT, GPx and the levels of GSH were declined significantly in APAP alone treated animals. Pretreatment with honey and silymarin prior to the administration of APAP significantly prevented the increase in the serum levels of hepatic enzyme markers and reduced oxidative stress. The histopathological evaluation of the livers also revealed that honey reduced the incidence of liver lesions induced by APAP. Results suggest that the Indian honey protects liver against oxidative damage and it could be used as an effective hepatoprotector against APAP induced liver damage.

scholarly journals IN-VITRO ANTIOXIDANT AND IN-VIVO HEPATO PROTECTIVE ACTIVITY OF ETHANOL EXTRACTS FROM THE BARK OF SHOREA ROBUSTA (DIPTEROCARPACEAE) AGAINST CARBON TETRA CHLORIDE INDUCED LIVER TOXICITY IN RATS

2016 ◽  
Vol 9 (6) ◽  
pp. 225
Author(s):  
Diptanu Biswas
Keyword(s):  
Liver Toxicity ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Protective Effects ◽  
Shorea Robusta ◽  
Carbon Tetra ◽  
Anti Oxidant ◽  
Ethanol Extracts

ABSTRACT: The study is designed for the evaluation of in-vivo Hepato protective and in-vitro Anti oxidant activity of ethanol extracts from the bark of Shorea robusta (Dipterocarpaceae) by CCl4 induced hepatotoxicity in rats. Ethanol extracts from the bark Shorea robusta (EESR) was evaluated for hepatoprotective activity in rats by inducing liver damage by CCl4. The anti oxidant activity of EESR was assayed by various in-vitro antioxidant methods and activities were compared to standard ascorbic acid. Ethanol extracts at an oral dose 200mg/kg and 400mg/kg exhibited a significant (*p<0.005) protective effects by lowering the level of SGOT, SGPT, ALP, Serum bilirubin, total cholesterol and increasing the level of total proteins as compared to Silymarin (50mg/kg) used as positive control. The extracts exhibit significant anti oxidant activity in various in vitro anti oxidant models.  From these studies we are concluding that, the ethanolic extracts of S.robusta have potent hepatoprotective effects and have anti oxidant properties, hence can be used as a natural product against liver damage.KEY WORDS: Anti oxidant, Carbon tetra chloride,  Hepatoprotective,  Shorea robusta

scholarly journals Evaluation of Hepatoprotective and in vivo Antioxidant Potentials of Methanol Extract of Tephrosia pumila against Thioacetamide Induced Liver Toxicity in Wistar Rats

2021 ◽  
Vol 70 (2) ◽  
Author(s):  
Ramesh C ◽  
Pinkey Rawal ◽  
Soma Pramanik ◽  
Shabana S
Keyword(s):  
Biochemical Parameters ◽  
Methanol Extract ◽  
Liver Toxicity ◽  
Antioxidant Properties ◽  
Total Duration ◽  
Hepatoprotective Activity ◽  
Oral Toxicity ◽  
Standard Drug ◽  
Protein Ions

The objective of the current investigation was performed to assess the hepatoprotective potentials and in vivo antioxidant properties of methanol extract of Tephrosia pumila against thioacetamide induced liver damage in rats. The acute oral toxicity study of methanol extract was determined as per OECD guidelines and the extract was proved to be safe up to the dose of 2000mg/kg. The total duration of the study was 21 days and animals were divided into six groups. Hepatotoxicity was induced in the animals of all groups except normal control by single dose administration of Thioacetamide(100mg/kg) at first day of the study followed by animals were treated daily with standard drug sylimarin and methanol extract of Tephrosia pumila (100mg/kg, 200mg/kg and 400mg/kg) to respective groups for 21 days. Variations in biochemical parameters like alanine transferase (ALT), aspartate transferase (AST), alkaline phosphatase (ALP), total bilirubin, direct bilirubin, albumin, total protein, ions and others parameters like clotting time and weight of the liver were considered to determine beneficial effect of the extract. At the end of the study liver samples were collected and subjected to histopathological evaluation. There were significant variations in the above mentioned biochemical parameters in toxic control animals treated with Thioacetamide alone while in the animals treated with methanol extract and standard drug silymarin, all the parameters were normal possibly due to their beneficial property in protecting the liver against thioacetamide induced hepatotoxicity. The results obtained in the above study suggesting that, the methanol extract of Tephrosia pumila possess significant hepatoprotective activity.

scholarly journals Secondary Metabolite Profiling, Anti-Inflammatory and Hepatoprotective Activity of Neptunia triquetra (Vahl) Benth

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7353
Author(s):  
Nasir Aziz Wagay ◽  
Shah Rafiq ◽  
Mohammad Aslam Rather ◽  
Younas Rasheed Tantray ◽  
Feng Lin ◽  
...  
Keyword(s):  
Total Protein ◽  
Ethanolic Extract ◽  
Liver Weight ◽  
Hepatoprotective Activity ◽  
Experimental Models ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Total Lipids ◽  
Anti Inflammatory

The present study aimed to analyze the phytoconstituents of Neptunia triquetra (Vahl) Benth. Anti-inflammatory and hepatoprotective activities of ethanol (EE), chloroform (CE) and dichloromethane (DCME) of stem extracts were evaluated using in vivo experimental models. The extracts were analyzed for phytoconstituents using GC-HRMS. Anti-inflammatory activity of CE, EE and DCME was accessed using carrageenan-induced paw oedema, cotton pellet-induced granuloma and the carrageenan-induced air-pouch model in Wistar albino rats. The hepatotoxicity-induced animal models were investigated for the biochemical markers in serum (AST, ALT, ALP, GGT, total lipids and total protein) and liver (total protein, total lipids, GSH and wet liver weight). In the in vivo study, animals were divided into different groups (six in each group) for accessing the anti-inflammatory and hepatoprotective activity, respectively. GC-HRMS analysis revealed the presence of 102 compounds, among which 24 were active secondary metabolites. In vivo anti-inflammatory activity of stem extracts was found in the order: indomethacin > chloroform extract (CE) > dichloromethane extract (DCME) > ethanolic extract (EE), and hepatoprotective activity of stem extracts in the order: CE > silymarin > EE > DCME. The results indicate that N. triquetra stem has a higher hepatoprotective effect than silymarin, however the anti-inflammatory response was in accordance with or lower than indomethacin.

scholarly journals Preliminary phytochemical screening and hepatoprotective activity of methanol extract of Artocarpus hirsutus leaves.

2016 ◽  
Vol 8 (3) ◽  
pp. 379
Author(s):  
Jitendra Patel ◽  
Venkateshwar Reddy ◽  
GS Kumar
Keyword(s):  
Total Bilirubin ◽  
Methanol Extract ◽  
Hepatoprotective Activity ◽  
Direct Bilirubin ◽  
Control Group ◽  
Phytochemical Screening ◽  
Methanolic Extracts ◽  
Group I ◽  
Hepatic Protection

<p>The objective of the present work was investigating the preliminary phytochemical screening and hepatoprotective activity of methanol extract of the leaves of <em>Artocarpus hirsutus</em>.<strong></strong></p><p>Group I served as vehicle control, Group II served CCL<sub>4 </sub>(2ml/kg, s.c.), Group III served as standard Silymarin (50 mg/kg, p.o.)  Group IV and V served as methanolic extracts of <em>Artocarpus hirsutus</em><strong> </strong>(MEAH) at the dose level (250 and 500 mg/kg, p.o.). The degree of protection was determined by measuring level biochemical marker like alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), direct bilirubin, total bilirubin and Cholesterol. The histopathology study also showed the hepatic protection of extracts. The preliminary phytochemical screening was performed to find out the phytoconstituents responsible for the activity.<strong></strong></p><p>The marker biochemical level such as ALT, AST, ALP, Direct bilirubin, Total bilirubin and Cholesterol were significantly raised in CCL<sub>4 </sub>treated rats when compared with the normal group (p&lt;0.05), but the MEAH (500 mg/kg, bw) treated rats exhibited maximum depletion. The histopathology study also showed the hepatic protection of extracts. Preliminary phytochemical screening showed the presence of glycosides, flavonoids, Tannins, triterpenoids, carbohydrates and steroids.</p><p>The results of <em>in vivo</em> hepatoprotective activity showed that the methanol extract of <em>Artocarpus hirsutus</em><strong> </strong>exhibit significant hepatoprotective activity. This might be due to flavonoids and tannins; which was confirmed their presence in phytochemical tests.  </p>

scholarly journals Phytochemical Characterization, Hepatoprotective Activity on Alcohol-Induced Toxicity of the Aqueous Extract of Curcuma longa (Zingiberaceae) in Wistar Rats

2021 ◽  
pp. 134-149
Author(s):  
Ameaka Fatima Nkempu ◽  
Tembe Estella ◽  
Tchadji Mayoudom Vanessa Edwige ◽  
Bayaga Herve ◽  
Dobgima John Fonmboh ◽  
...  
Keyword(s):  
Liver Disease ◽  
Secondary Metabolites ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Liver Toxicity ◽  
Curcuma Longa ◽  
Hepatoprotective Activity ◽  
Bioactive Metabolites ◽  
Central Vein ◽  
Phytochemical Screening

Introduction: Liver or hepatic disease refers to different conditions that affect the liver. Chronic alcohol consumption is one of the most frequent causes of liver disease and accounts for about 55% of liver cirrhosis deaths recorded in Cameroon in 2020. Standard accessible treatments focus on end-stage liver disease with safety and efficacy obstacles. We have a research gap in Cameroon to understand the alternative use of natural products as treatment with a long traditional history of safe use. Curcuma longa has long been a source of traditional and modern medicine. It is commonly used in Cameroon as a spice and herbal product with some level of activity against various forms of liver disease. Objective: To phytochemically screen for bioactive metabolites and evaluate the hepatoprotective activity of the aqueous extract of Curcuma longaon alcohol-induced toxicity in Wistar rats. Methods: Phytochemical screening was carried out on the aqueous extract obtainedfrom maceration of plant rhizomes. Three doses (125, 250 and 500mg/Kg) of the plant extract and the reference (Silymarin 50mg/Kg) were administered daily (p.o) to rats 30 min before administration of 40% alcohol (2mL/100g p.o) for 21 days. Biochemical parameters such as ALAT, ASAT, GGT, Bilirubin and Lipid profile were quantified and histological studies of the liverwas carried out using standard procedures. Results: Phytochemical screening of the aqueous extract of C. longa revealed polyphenols such as flavonoids, tannins, quinones, saponins and phlobatanins. The plant showed hepatoprotective activity by decreasing liver toxicity markers such as ASAT, ALAT, GGT and Bilirubin. Histology revealed dose-dependent protection with 500 mg/Kg showing the most cellular integrity, no central vein occlusion and minimal fibrosis. Conclusion: This study indicated the presence of polyphenols like flavonoids and tannins in the aqueous extract of C. longa. The presence of these secondary metabolites in the studied extract justifies its antioxidant and anti-inflammatory properties confirmed by its hepatoprotective effects on alcohol-induced toxicity. This was clearly shown by biochemical and histological parameters. More sensitive and specific methods are required to test for these secondary metabolites in serum.

scholarly journals Hepatoprotective activity of the ethanolic extract of Morus indica roots from Indian Bodo tribes

2022 ◽  
Vol 4 (2) ◽  
Author(s):  
Hankhray Boro ◽  
Talambedu Usha ◽  
Dinesh Babu ◽  
Prakashmurthy Chandana ◽  
Arvind Kumar Goyal ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Active Components ◽  
Traditional Use ◽  
Hematological Indices ◽  
Pharmacological Mechanism ◽  
Hepatic Lipid Peroxidation

AbstractThe roots of Morus species are well described in the Pharmacopoeia of the People's Republic of China (ChP) for its traditional use in treating liver fibrosis due to its hepatoprotective property. However, little is known about the hepatoprotective effect of the roots of Morus indica L. (RoMi), and the pharmacological mechanism(s) are uncertain due to its intricacy. Therefore, this study evaluates the hepatoprotective activity of the ethanolic extract of RoMi (eRoMi) against the CCl4-induced in-vivo animal model at different dosages (100 and 200 mg/kg BW) in comparison with silymarin as a positive control. The hepatoprotective activity of eRoMi was evaluated by measuring the levels of serum biomarkers, hepatic antioxidant enzymes and was verified by histological studies. Interestingly, 1,2-bis(trimethylsilyl) benzene, 1,4-phenylenebis (trimethylsilane), 2,4,6-cycloheptatriene-1-one, 3,5-bis-trimethylsilyl and α-amyrin were the active components found in eRoMi as detected by GC–MS. Oral administration of eRoMi (200 mg/kg BW) to rats significantly protected serum biochemical parameters (increased ALT, AST, LDH, bilirubin and GGT as well as depletion of antioxidant enzymes and hepatic GSH) and elevation in hepatic lipid peroxidation as compared to CCl4-treated rats. The hematological indices such as erythrocytes, hemoglobin, monocytes and lymphocytes were also normal in eRoMi-treated rats. The histopathological evaluation indicated a significant restoration of liver structure as compared to silymarin. This study is the first scientific validation for the traditional use of eRoMi to understand its hepatoprotective activity.

scholarly journals PHYTOCHEMICAL ANALYSIS, ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITY OF ACTINIOPTERIS RADIATA

2020 ◽  
pp. 69-74
Author(s):  
KANDUKURI GOURI SANKAR ◽  
BENDI SRI VENKATESWARLU
Keyword(s):  
Antioxidant Activity ◽  
Alkaline Phosphatase ◽  
Free Radical ◽  
Medicinal Plants ◽  
Total Bilirubin ◽  
Liver Toxicity ◽  
Hepatoprotective Activity ◽  
Phytochemical Analysis ◽  
Alcoholic Extract ◽  
Phytochemical Constituents

Objective: The medicinal plants have been using to treat ailments since ancient times. The recent advances in science and technology impel humans to evaluate medicinal plants therapeutic efficiency and isolation of bioactive compounds in pure forms before their use in development of new drugs and their derivatives. But even now, abundant medicinal plants unevaluated scientifically. The current study was aimed to explore phytochemical constituents, antioxidant and hepatoprotective activities of Actiniopteris radiata root parts. Methods: Standard procedures have been used to perform phytochemical analysis. Antioxidant activity was carried using In vitro methods on superoxide, hydroxyl, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. Hepatoprotective activity was studied by paracetamol-induced liver toxicity on WISTAR albino rats. The parameters assessed were Aspartate aminotransferase (SGOT/AST), Alanine aminotransferase (SGPT/ALT), alkaline phosphatase (ALP) and total bilirubin levels. Results: The tested extracts (hexane, ethyl acetate, and hydro-alcoholic) possess biologically active compounds such as sterols, terpenoids, glycosides, phenolics, alkaloids, flavonoids. The hydro-alcoholic extract has more phenolic contents (24.28±0.3) and flavonoid contents (22.68±0.6). The extracts showed dose dependent activity on tested free radicals and extracts showed more percentage inhibition at 320µg. The hydro-alcoholic extract showed more percentage inhibition i.e. 71.00±2.08 on DPPH free radical, 79.67±1.20 on hydroxyl free radical and 80.33±1.20 on superoxide free radical. As antioxidant activity of hexane and ethyl acetate extracts was less and they also showed less percentage protection on liver toxicity, hydro-alcoholic extract showed more percentage protection on biomedical enzyme levels of liver toxicity at high concentration i.e., 400 mg/kg b.w. The percentage protection on the enhancement of AST (SGOT), ALT (SGPT), ALP, and total bilirubin levels were 82.24%, 82.14%, 84.18%, and 82.85% are significant (P<0.01) as Liv52 shown percentage protection on the enhancement of Aspartate aminotransferase (SGOT), alanine aminotransferase (SGPT), Alkaline phosphatase (ALP) and total bilirubin levels were 93.58%, 92.83%, 94.67% and 93.57%. Conclusion: The current study was aimed to explore phytochemical constituents, antioxidant and hepatoprotective activities of Actiniopteris radiata root parts extracts. The outcome of the current research results provides scientific evidence of the traditional usage of Actiniopteris radiata.

scholarly journals Evaluation of Hepatoprotective Effect of Leaves ofCassia sopheraLinn.

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Arijit Mondal ◽  
Sanjay Kumar Karan ◽  
Tanushree Singha ◽  
D. Rajalingam ◽  
Tapan Kumar Maity
Keyword(s):  
Carbon Tetrachloride ◽  
Total Protein ◽  
Total Bilirubin ◽  
Serum Alkaline Phosphatase ◽  
Histopathological Examination ◽  
Elevated Serum ◽  
Hepatoprotective Activity ◽  
Hepatic Damage ◽  
Protein Levels ◽  
Serum Glutamate

In the present study, the hepatoprotective activity of ethanolic extracts ofCassia sopheraLinn. leaves was evaluated against carbon-tetrachloride- (CCl4-) induced hepatic damage in rats. The extracts at doses of 200 and 400 mg/kg were administered orally once daily. The hepatoprotection was assessed in terms of reduction in histological damage, changes in serum enzymes, serum glutamate oxaloacetate transaminase (AST), serum glutamate pyruvate transaminase (ALT), serum alkaline phosphatase (ALP), total bilirubin, and total protein levels. The substantially elevated serum enzymatic levels of AST, ALT, ALP, and total bilirubin were restored towards the normalization significantly by the extracts. The decreased serum total protein level was significantly normalized. Silymarin was used as standard reference and exhibited significant hepatoprotective activity against carbon tetrachloride-induced hepatotoxicity in rats. The biochemical observations were supplemented with histopathological examination of rat liver sections. The results of this study strongly indicate thatCassia sopheraleaves have potent hepatoprotective action against carbon tetrachloride-induced hepatic damage in rats. This study suggests that possible activity may be due to the presence of flavonoids in the extracts.

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