Quantification of Flavonoids in Alpinia officinarum Hance. via HPLC and Evaluation of its Cytotoxicity on Human Prostate Carcinoma (LNCaP) and Breast Carcinoma (MCF-7) Cells

Background: Various plant species have been shown to be effective in prevention or adjuvant therapy of cancer. Alpinia officinarum and its main phytochemicals have also been the subject of several studies for their anti-cancer properties. Objective: The objective of this study is to analyze the extracts of A. officinarum to quantify flavonoids, and to evaluate the growth inhibitory effects of the extracts on MCF-7 and LNCaP cells. Methods: A. officinarum aqueous and hydroalcoholic extracts were analyzed using high-performance liquid chromatography (HPLC) for quantification of three flavonoid compounds. Then MCF-7, LNCaP, and fibroblast cells were treated with several concentrations (25, 50, 100, 200, and 400 μg/mL) of extracts (24, 48 and 72h). Cell viability was assessed using MTT assay. Flow cytometry was conducted to evaluate apoptosis. Results: Galangin and kaempferol (3.85 and 1.57 mg/g dry extract) were quantified respectively in hydroalcoholic and aqueous extracts using a validated method. The hydroalcoholic extract significantly decreased the viability of MCF-7 (IC50: 43.45μg/mL for 48h) and LNCaP cells (IC50: 168μg/mL for 48h). The aqueous extract reduced cancer cell viability by more than 50% only at 200 and 400 μg/mL (72h). Treatment of primary fibroblasts with both extracts showed no significant decrease in cell viability (25-100 μg/mL; 24 and 48h). The hydroalcoholic extract induced a significant increase in apoptotic cells in both MCF-7 and LNCaP cells. Conclusion: Obtained results demonstrated the cytotoxicity of A. officinarum through apoptosis induction in two cancer cell lines. Further investigations are required to determine the underlying apoptotic cell death mechanisms induced by A. officinarum in cancerous cells.

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Analyzing Cytotoxic and Apoptogenic Properties ofScutellaria litwinowiiRoot Extract on Cancer Cell Lines

Evidence-based Complementary and Alternative Medicine ◽

10.1093/ecam/nep214 ◽

2011 ◽

Vol 2011 ◽

pp. 1-9 ◽

Cited By ~ 15

Author(s):

Zahra Tayarani-Najaran ◽

Seyed Ahmad Emami ◽

Javad Asili ◽

Alireza Mirzaei ◽

Seyed Hadi Mousavi

Keyword(s):

Flow Cytometry ◽

Cell Lines ◽

Cancer Cell ◽

Methylene Chloride ◽

Apoptotic Cell ◽

Malignant Cell ◽

Cancer Cell Lines ◽

Dependent Manner ◽

Antitumor Effects ◽

Mcf 7

TheScutellariaspecies (Lamiaceae) is used as a source of flavonoids to treat a variety of diseases in traditional medicine. In spite of many reports about the cytotoxic and antitumor effects of some species of this genus, anticancer researches on one of the Iranian speciesS. litwinowiihave not yet been conducted.The cytotoxic properties of total methanol extract ofS. litwinowiiand its fractions were investigated on different cancer cell lines including AGS, HeLa, MCF-7, PC12 and NIH 3T3. Meanwhile, the role of apoptosis in this toxicity was explored. The cells were cultured in DMEM medium and incubated with different concentrations of herb plant extracts. Cell viability was quantitated by MTT assay. Apoptotic cells were determined using propidium iodide staining of DNA fragmentation by flow cytometry (sub-G1 peak).Scutellaria litwinowiiinhibited the growth of malignant cells in a dose-dependent manner. Among solvent fractions ofS. litwinowii, the methylene chloride fraction was found to be more toxic compared to other fractions. The IC50values of this fraction against AGS, HeLa, MCF-7 and PC12 cell lines after 24 h were determined, 121.2 ± 3.1, 40.9 ± 2.5, 115.9 ± 3.5 and 64.5 ± 3.4μg/ml, respectively.Scutellaria litwinowiiinduced a sub-G1 peak in the flow cytometry histogram of treated cells compared to control cells indicating that apoptotic cell death is involved inS. litwinowiitoxicity.Scutellaria litwinowiiexerts cytotoxic and proapototic effects in a variety of malignant cell lines and could be considered as a potential chemotherapeutic agent in cancer treatment.

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Potential Metabolite Nymphayol Isolated from Water Lily (Nymphaea stellata) Flower Inhibits MCF-7 Human Breast Cancer Cell Growth via Upregulation of Cdkn2a, pRb2, p53 and Downregulation of PCNA mRNA Expressions

Metabolites ◽

10.3390/metabo10070280 ◽

2020 ◽

Vol 10 (7) ◽

pp. 280

Author(s):

Laila Naif Al-Harbi ◽

Pandurangan Subash-Babu ◽

Manal Abdulaziz Binobead ◽

Maha Hussain Alhussain ◽

Sahar Abdulaziz AlSedairy ◽

...

Keyword(s):

Breast Cancer ◽

Tumor Suppressor ◽

Cell Viability ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Kinase Inhibitor ◽

B Cell Lymphoma ◽

Nuclear Antigen ◽

Mrna Levels ◽

Mcf 7

Controlled production of cyclin dependent kinases (CDK) and stabilization of tumor suppressor genes are the most important factors involved in preventing carcinogenesis. The present study aimed to explore the cyclin dependent apoptotic effect of nymphayol on breast cancer MCF-7 cells. In our previous study, we isolated the crystal from a chloroform extract of Nymphaea stellata flower petals and it was confirmed as nymphayol (17-(hexan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol) using x-ray diffraction (XRD), Fourier transform infrared (FTIR), and mass spectroscopy (MS) methods. The cytotoxic effect of nymphayol on MCF-7 cells were analyzed using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The cellular and nuclear damage was determined using propidium iodide (PI) and acridine orange/ethidium bromide (AO/ErBr) staining. Tumor suppressor and apoptosis related mRNA transcript levels were determined using real-time polymerase chain reaction (RT-PCR). Nymphayol potentially inhibits MCF-7 cell viability up to 78%, and the IC50 value was observed as 2.8 µM in 24 h and 1.4 µM in 48 h. Treatment with nymphayol significantly increased reactive oxygen species (ROS) level and the tunnel assay confirmed DNA damage. We found characteristically 76% apoptotic cells and 9% necrotic cells in PI and AO/ErBr staining after 48 h treatment with 2.8 µM of nymphayol. Gene expression analysis confirmed significantly (p ≤ 0.001) increased mRNA levels of cyclin dependent kinase inhibitor 2A (Cdkn2a), retinoblastoma protein 2 (pRb2), p53, nuclear factor erythroid 2-factor 2 (Nrf2), caspase-3, and decreased B-cell lymphoma 2 (Bcl-2), murine double minute 2 (mdm2), and proliferating cell nuclear antigen (PCNA) expression after 48 h. Nymphayol effectively inhibited breast cancer cell viability, and is associated with early expression of Cdkn2a, pRb2, and activation of p53 and caspases.

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Effect of Hydroalcoholic Extract of Ephedramajor, Memordia charantia, and Resveratrol on Cytotoxicity and Caspase-3 Genes Expression Level in MCF-7 Breast Cancer Cell Line

Gene Cell and Tissue ◽

10.5812/gct.110658 ◽

2021 ◽

Vol In Press (In Press) ◽

Author(s):

Seyed Kazem Sabbagh ◽

Ehsan Ghodrati ◽

Alireza Hajibeiki ◽

Mahta Mazaheri ◽

Mohammad Reza Sarafraz Ardakani ◽

...

Keyword(s):

Gene Expression ◽

Medicinal Plants ◽

Cell Viability ◽

Cell Line ◽

Plant Extracts ◽

Caspase 3 ◽

Cell Toxicity ◽

Hydroalcoholic Extract ◽

Expression Levels ◽

Mcf 7

Background: To increase the therapeutic effect of drugs to combat diseases, combination therapy with current chemical drugs and new medicines derived from medicinal plants is necessary. Objectives: The present work aimed to investigate the effect of hydroalcoholic extract of two medicinal plants, Ephedra major and Momordi cacharantia (Carla), and resveratrol drug on cell viability and expression levels of caspase-3 gene in MCF-7 cell line. Methods: In this experimental study, the hydroalcoholic extraction of tested plants was done with a Soxhlet extractor. The MTT assay and real-time PCR were used to determine cell toxicity and caspase-3 gene expression levels, respectively. Results: The highest and lowest cytotoxic effects of plant extracts and resveratrol were observed at concentrations of 500 and 150 µg/mL, respectively. The highest level of the caspase-3 gene expression was observed after 72 h of incubation by different concentrations of plant extracts and resveratrol. Conclusions: It can be concluded that both plant extracts could influence cell viability in MCF-7 cells via the increase of cell toxicity and expression of caspase3 gene. Thus, these species could be used in the pharmaceutical industry.

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Comparison on the Cancer Specific Cytotoxicity of Three Gingers (Zingiber officinale Rosc) Leaves Varieties from Indonesia

International Journal of Pharmacognosy and Phytochemical Research ◽

10.25258/ijpapr.v9i1.8053 ◽

2017 ◽

Vol 9 (1) ◽

Author(s):

Susanti S. ◽

Kumoro C. A. ◽

Santoso I. S. ◽

Murwanti R. ◽

Suzery M. ◽

...

Keyword(s):

Cell Viability ◽

Cancer Cell ◽

Detrimental Effect ◽

Zingiber Officinale ◽

Assay Method ◽

Human Breast ◽

Normal Cells ◽

Specific Cytotoxicity ◽

Reduce Cell Viability ◽

Mcf 7

This study was performed to get more insight the cancer specific cytotoxicity of ginger leaf (GL). Three GL varieties (Gajah, Emprit, and Red) were extracted and fractionated. Each etyl acetate fraction in concentration of 200 µg/ml was tested about specific cytotoxicity toward cancer dan normal cells. Cancer cell lines used in this study were human colorectal (HCT116) and human breast (MCF-7 and T47D) cancer while normal cell line was human fibroblast (KMST-6). Based MTS assay method, the results showed Gajah and Emprit GL more significantly reduce cell viability of HCT116 and T47D than Red GL although there was no difference on the efficacy of both varieties. All varieties of GL also significantly reduce cell viability of MCF-7 compare to PBS control. However, there were not significant differences between those GL varieties on their effectiveness against MCF-7. In contrast, there were no effects on the KMST-6 due to all GL varieties treatment compare with PBS control. All data suggested that GL treatment only inhibited in the cancer cells without detrimental effect in the normal cells. Effectiveness of GL against cancer cell varies depend on the varieties. Gajah and Emprit GL are better varieties possess the cancer specific cytotoxicity that merits to be developed as promising chemo preventive agent in the future.

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Comparative Assessment of the Volatile Profile, Antioxidant Capacity and Cytotoxic Potential of Different Preparation of Millefolli Herba Samples

Revista de Chimie ◽

10.37358/rc.20.3.7975 ◽

2001 ◽

Vol 71 (3) ◽

pp. 69-78

Author(s):

Mihaela Buleandra ◽

Zenovia Moldovan ◽

Irinel Adriana Badea ◽

Iulia Gabriela David ◽

Dana Elena Popa ◽

...

Keyword(s):

Cell Death ◽

Essential Oil ◽

Cell Viability ◽

Antioxidant Capacity ◽

Apoptotic Cell ◽

Principal Component ◽

Volatile Components ◽

Dependent Manner ◽

Hydroalcoholic Extract ◽

Hct 116

Millefolii herba is an available product on the Romanian market as mixture of stems, leaves and flowers of Achillea millefolium L. There were established its volatile compounds profile, total polyphenolic content (TPC), antioxidant capacity and effects on HCT 116 cell viability and programmed cell death. The infusion, hydroalcoholic extract and hydrodistillated essential oil were studied. A comparative analysis using static headspace (HS) and hydro-distillation (HD) GC/MS of the volatile components from Millefolii herba was realized: the essential oil contains chamazulene as the principal component (37.1%), while 1,8-cineole (46.8%) is the main constituent of headspace volatiles. The highest antioxidant capacity was found in essential oil, compared with hydroalcoholic extract, infusion and ascorbic acid. Yarrow hydroalcoholic extract reduced the HCT 116 cell viability and induced the apoptotic cell death in a dose and time dependent manner.

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Zerumbone inhibits prostate cancer cell viability and induces cell death by non-apoptotic pathway

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v11i4.26728 ◽

2016 ◽

Vol 11 (4) ◽

pp. 771

Author(s):

Xian-De Cao ◽

Hui-Min Zheng

Keyword(s):

Prostate Cancer ◽

Cell Death ◽

Cell Viability ◽

Cancer Cell ◽

Propidium Iodide ◽

Prostate Cancer Cell ◽

Apoptotic Cell ◽

D1 Protein ◽

Annexin V ◽

Apoptotic Pathway

<p class="Abstract">The aim of the present study was to investigate the role of zerumbone on the proliferation, cell cycle arrest and cell death in DU-145 prostate cancer cell lines. The MTT assay revealed that zerumbone (20 µM) reduced proliferation of DU-145 cells to 39.0% at 48 hours. It also increased the proportion of propidium iodide stained cells to 53.4% compared 1.0% in control. However, the population of annexin V-stained cells remained uneffected indicating induction of non-apoptotic cell death by zerumbone. Treatment of DU-145 cells with zerumbone (20 µM) caused 8-fold enhancement in the level of reactive oxygen species (ROS). On the other hand, exposure of the zerumbone treated DU-145 cells to glutathione inhibited the generation of ROS. Fow cytometry using propidium iodide staining revealed that zerumbone treat-ment increased proportion of cells in G1 phase to 71.3% on compared to 34.7% in the control. The results from Western blot analysis revealed a significant increase in the expression of cyclin D1 protein in DU-145 cells on treatment with 20 µM concentration of zerumbone. Thus, zerumbone treatment inhibits prostate cancer cell viability and can be used for its treatment.</p><p> </p>

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Biological Effect of Organically Coated Grias neuberthii and Persea americana Silver Nanoparticles on HeLa and MCF-7 Cancer Cell Lines

Journal of Nanotechnology ◽

10.1155/2018/9689131 ◽

2018 ◽

Vol 2018 ◽

pp. 1-11 ◽

Cited By ~ 6

Author(s):

Lizeth Salazar ◽

María José Vallejo López ◽

Marcelo Grijalva ◽

Luis Castillo ◽

Alexander Maldonado

Keyword(s):

Gene Expression ◽

Transcription Factor ◽

Cell Viability ◽

Cell Lines ◽

Cancer Cell ◽

Hela Cells ◽

Biological Effect ◽

Persea Americana ◽

Expression Levels ◽

Mcf 7

The aim of this study was to assess the biological effect of organically coated Grias neuberthii (piton) fruit and Persea americana (avocado) leaves nanoparticles (NPs) on cervical cancer (HeLa) and breast adenocarcinoma (MCF-7) cells with an emphasis on gene expression (p53 transcription factor and glutathione-S-transferase GST) and cell viability. UV-Vis spectroscopy analysis showed that synthesized AgNPs remained partially stable under cell culture conditions. HeLa cells remained viable when exposed to piton and avocado AgNPs. A statistically significant, dose-dependent cytotoxic response to both AgNPs was found on the breast cancer (MCF-7) cell line at concentrations above 50 µM. While expression levels of transcription factor p53 showed downregulation in treated MCF-7 and HeLa cells, GST expression was not affected in both cell lines treated. Cell viability assays along with gene expression levels in treated MCF-7 cells support a cancer cell population undergoing cell cycle arrest. The selective toxicity of biosynthesized piton/avocado AgNPs on MCF-7 cells might be of value for novel therapeutics.

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The Synergistic Combination of Cisplatin and Piperine Induces Apoptosis in MCF-7 Cell Line

Iranian Journal of Public Health ◽

10.18502/ijph.v50i5.6121 ◽

2021 ◽

Author(s):

Abolfazl Fattah ◽

Ali Morovati ◽

Zahra Niknam ◽

Ladan Mashouri ◽

Amirhooman Asadi ◽

...

Keyword(s):

Breast Cancer ◽

Cell Viability ◽

Cancer Cells ◽

Cell Line ◽

Cancer Cell ◽

Breast Cancer Cells ◽

Caspase 3 ◽

Assay Method ◽

Caspase 9 ◽

Mcf 7

Background: Piperine is a natural compound obtained from the Piper nigrum that exhibits anti-proliferative and anti-cancer activity in cancer cell lines. We analyzed the cytotoxic effect of piperine combined with cisplatin compound in the human MCF-7 breast cancer cell line and the underlying mechanism. Methods: The present in vitro study was performed on MCF-7 cell line in Jahrom University of Medical Sciences between, Jahrom, Iran from 2016 to 2017. Cultured MCF-7 cells were seeded into four groups: a control group (untreated group), a group treated with cisplatin, a group treated with piperine and a group treated with cisplatin and piperine. Cell viability was analyzed using the MTT assay method. Flow c-ytometric analysis was investigated for apoptosis. The mRNA and protein expression of the apoptotic regulators p53, Bcl-2, Bax, caspase 3 and caspase 9 were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting analysis. Results: Piperine (20 and 30 µM) in combination with cisplatin (5, 10 and 15 µM) for 24 h synergistically inhibited cell viability of MCF-7 breast cancer cells more than piperine and cisplatin used alone. Synergistic antibreast cancer activities cisplatin (5 µM) and piperine (20 µM) were via inducing apoptosis. Piperine (20 µM) and cisplatin (5 µM) for 24 h induce apoptosis strongly through reduction of Bcl-2 and increase of caspase 3, p53, caspase 9, and Bax. Conclusion: Piperine in combination with cisplatin could trigger p53-mediated apoptosis more effective than cisplatin alone in MCF-7 breast cancer cells, reducing the toxic dose of cisplatin used in cancer chemotherapy.

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PIPERINE IN THE PREVENTION OF THE DECREASED TAMOXIFEN SENSITIVITY IN MCF-7 BREAST CANCER CELL LINE

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2018.v10s1.74 ◽

2018 ◽

Vol 10 (1) ◽

pp. 335

Author(s):

Sandy Vitria Kurniawan ◽

Lies Sugiarti ◽

Septelia Inawati Wanandi ◽

Melva Louisa

Keyword(s):

Breast Cancer ◽

Cell Viability ◽

Cancer Cells ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Breast Cancer Cells ◽

Cancer Cell Line ◽

P Glycoprotein ◽

Mcf 7 ◽

In Cells

Objective: This study was designed to analyze the role of piperine in modulating P-glycoprotein mRNA expression when added in combination withtamoxifen to breast cancer cells in culture.Methods: MCF-7 breast cancer cells were treated with 1 μM tamoxifen with or without piperine (12.5, 25, or 50 μM) or verapamil 50 μM (P-glycoproteininhibitor positive control) for up to 12 days. We assessed the cell viability and isolated total RNA from them. We quantified P-glycoprotein expressionsusing quantitative reverse transcription polymerase chain reaction.Results: Administration of various doses of piperine decreased MCF-7 breast cancer cell viability. Piperine, when given in combination with tamoxifen,decreased the expression of P-glycoprotein mRNA in cells compared with the expression in cells treated with tamoxifen only. The effects were shownto be dose dependent.Conclusion: Piperine prevents the development of breast cancer cell tamoxifen resistance, probably through its inhibition of P-glycoprotein expression.

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