Chrysin Impact on Oxidative and Inflammation Damages in the Liver of Aged Male Rats

2020 ◽  
Vol 20 ◽  
Author(s):  
Mohammad Samini ◽  
Tahereh Farkhondeh ◽  
Mohsen Azimi-Nezhad ◽  
Saeed Samarghandian
Keyword(s):  
Lipid Peroxidation ◽  
Natural Antioxidants ◽  
Serum Levels ◽  
Treated Group ◽  
Male Rats ◽  
Hepatic Tissue ◽  
Control Group ◽  
Middle Aged ◽  
Tnf Α ◽  
Male Wistar Rats

Aims: The purpose of this research was to investigate the effect of chrysin on one of the natural antioxidants on aging progression in the animal model. Background: Oxidative stress and inflammation increase in hepatic tissue during aging, leading to liver dysfunction. Objective: The current research was conducted to show the effect of chrysin on the activities of antioxidant enzyme (catalase, glutathione peroxidase, and superoxide dismutase), serum nitric oxide (NO), and lipid peroxidation as well as inflammatory cytokines (TNF-α, IL-6, and IL-1β) of aging rats. Method: Male Wistar rats of different ages, 2, 10, and 20 months randomly divided into six groups as follows (n=8, per each group): young control rats (C2), young CH-treated rats (CH2), middle-aged control rats (C10), middle-aged CH-treated group (CH10), aged control group (C20), and aged CH-treated group (CH20). Chrysin (20 mg/kg) was administrated intraperitoneally once a day for 30 days. Result: Present findings indicated that chrysin treatment ameliorated the increased liver levels of lipid peroxidation, TNF-α, and IL-1β as well as serum levels of NO. Conclusion: The findings suggest that chrysin could be effective against the progression of age-induced damage by modulation oxidant-antioxidant system and inflammatory response.

scholarly journals Metallothionein-II Inhibits Lipid Peroxidation and Improves Functional Recovery after Transient Brain Ischemia and Reperfusion in Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Araceli Diaz-Ruiz ◽  
Patricia Vacio-Adame ◽  
Antonio Monroy-Noyola ◽  
Marisela Méndez-Armenta ◽  
Alma Ortiz-Plata ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Frontal Cortex ◽  
Neurological Deficit ◽  
Neuroprotective Effect ◽  
Treated Group ◽  
Neurological Deficits ◽  
Control Group ◽  
Ischemia And Reperfusion ◽  
Male Wistar Rats ◽  
Neuroprotective Treatment

After transient cerebral ischemia and reperfusion (I/R), damaging mechanisms, such as excitotoxicity and oxidative stress, lead to irreversible neurological deficits. The induction of metallothionein-II (MT-II) protein is an endogenous mechanism after I/R. Our aim was to evaluate the neuroprotective effect of MT-II after I/R in rats. Male Wistar rats were transiently occluded at the middle cerebral artery for 2 h, followed by reperfusion. Rats received either MT (10 μg per rat i.p.) or vehicle after ischemia. Lipid peroxidation (LP) was measured 22 h after reperfusion in frontal cortex and hippocampus; also, neurological deficit was evaluated after ischemia, using the Longa scoring scale. Infarction area was analyzed 72 hours after ischemia. Results showed increased LP in frontal cortex (30.7%) and hippocampus (26.4%), as compared to control group; this effect was fully reversed by MT treatment. Likewise, we also observed a diminished neurological deficit assessed by the Longa scale in those animals treated with MT compared to control group values. The MT-treated group showed a significant (P<0.05) reduction of 39.9% in the infarction area, only at the level of hippocampus, as compared to control group. Results suggest that MT-II may be a novel neuroprotective treatment to prevent ischemia injury.

scholarly journals Neurotoxic Effects of Stanozolol on Male Rats‘ Hippocampi: Does Stanozolol cause apoptosis?

2019 ◽  
Vol 10 (1) ◽  
pp. 73-81
Author(s):  
Faezeh Nemati Karimooy ◽  
Alireza Ebrahimzadeh Bideskan ◽  
Abbas Mohammadi Pour ◽  
Seyed Mahmoud Hoseini
Keyword(s):  
Histopathological Examination ◽  
Apoptotic Cell ◽  
Cell Formation ◽  
Treated Group ◽  
Male Rats ◽  
Control Group ◽  
Cell Detection ◽  
Apoptotic Effect ◽  
Male Wistar Rats ◽  
The Mean

AbstractStanozolol is an anabolic-androgenic steroid which is commonly abused by athletes for improved energy, appearance, and physical size. It has been previously shown to cause changes in behaviour and has various physical effects. Studies have previously been conducted on its neurotoxic effect on the central nervous system (CNS), which are typically psychological in nature. This study was performed to investigate the apoptotic effect of stanozolol on different parts of the rat hippocampus. Sixteen male Wistar rats were divided randomly into two groups (experimental and control). The experimental group received subcutaneous injections of stanozolol (5mg/kg/day) for consecutive 28 days, whereas the control group received saline using the same dosing schedule and administration route. After routine procedures, coronal sections of rat brain were stained with Toluidine blue and TUNEL for pre-apoptotic and apoptotic cell detection, respectively. In order to compare groups, the mean number of TUNEL-positive and pre-apoptotic neurons per unit area were calculated and analysed. Histopathological examination revealed that the mean number of pre-apoptotic and apoptotic neurons in the CA1, CA2, CA3 and DG areas of the hippocampus were significantly increased in the stanozolol treated group. In conclusion, stanozolol abuse may induce pre-apoptotic and apoptotic cell formation in different regions of the hippocampus.

Effects of curcumin on antioxidant capacity and gastric mucosal injury following strenuous endurance training in rats

2020 ◽  
pp. 1-8
Author(s):  
A. Gorzi ◽  
M. Asadi ◽  
F.A. Voltarelli ◽  
M. Molanouri Shamsi
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Endurance Training ◽  
Serum Levels ◽  
Mucosal Injury ◽  
Gastric Mucosal Injury ◽  
Male Rats ◽  
Antioxidant Defences ◽  
Gastrointestinal Problems ◽  
Male Wistar Rats

Strenuous endurance training (SET) in endurance athletes can cause gastrointestinal problems, such as gastritis. Gastritis is associated with increased oxidative stress and an imbalance between free-radical production and antioxidant defences. The present study investigated the effects of SET and curcumin injection on systemic total antioxidant capacity (TAC), gastric mucosal injury and lipid peroxidation (malondialdehyde – MDA) in male rats. Twenty-six male Wistar rats were randomly divided into four groups including Control, Curcumin, Endurance, and Endurance + curcumin. Incremental endurance training (up to 70 min with 35 m/min), and curcumin injection (30 mg/kg bodyweight, three times per week) was carried out in relevant groups. The pathology of gastritis was measured concerning the restoration factors (the number of neutrophils, the formation of new vessels, and proliferation of fibroblasts), gastric MDA levels and the TAC were measured. SET-induced gastritis symptoms, such as gastric mucosal injury and lipid peroxidation and curcumin decreased systemic TAC. However, curcumin reduced exercise-induced gastric mucosal injury and lipid peroxidation. Also, serum levels of TAC were maintained at normal levels following a combination of SET and curcumin injection. These findings suggest that curcumin injection during SET could be useful for managing gastritis symptoms and improving antioxidant capacity in healthy and eutrophic rats.

Effect of Sodium Metabisulfite on Oxidative Stress and Lipid Peroxidation Biomarkers

2020 ◽  
Vol 16 (1) ◽  
pp. 114-117
Author(s):  
Shahnaz Shekarforoush ◽  
Parisa Ebrahimi ◽  
Akbar Afkhami Fathabad ◽  
Elaheh Farzanfar
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Treated Group ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Sodium Metabisulfite ◽  
Male Wistar Rats ◽  
Pharmaceutical Agents ◽  
Cellular Components ◽  
Dose Dependent

Background: Sulfites are widely used as preservatives in the foods and pharmaceutical agents. It has been demonstrated that sulfites can react with a variety of cellular components and cause toxicity. Objective: The present study was designed to investigate the effects of ingested sodium metabisulfite (SMB) on serum antioxidant status in rats. Methods: Thirty-two male Wistar rats were randomly divided into control and treated groups. Treated groups received 10, 100, and 260 mg/kg body weight of SMB for 28 days. After 28 days, serum was assayed for measuring superoxide dismtase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT) activities, glutathion (GSH) level and lipid peroxidation. Results: The results showed that the activities of GPx, GR, CAT and GSH levels were significantly decreased in 100 and 260 mg/kg SMB treated rats, while malondialdehyde (MDA) level was significantly increased in 260 mg/kg treated group when compared with the control group. Conclusion: It is concluded that SMB administration as dose-dependent is associated with decreased serum antioxidant enzyme activities and increased lipid peroxidation.

scholarly journals Evaluating the Effects of Noise Pollution on the Levels of Blood Cortisol, Testosterone, and Thyroid in Male Wistar Rats

Health Scope ◽  
2021 ◽  
Vol 10 (3) ◽  
Author(s):  
Seyed-Hosein Abtahi-Eivary ◽  
Ali Tajpoor ◽  
Ali Firoozi ◽  
Shahrzad Mehrzad ◽  
Mohammad Hosein Beheshti
Keyword(s):  
Wistar Rats ◽  
Statistical Tests ◽  
Noise Pollution ◽  
Well Being ◽  
Serum Levels ◽  
Sound Level ◽  
Male Rats ◽  
Control Group ◽  
Male Wistar Rats ◽  
Commercial Kits

Background: Noise pollution is a global problem causing changes in the secretion of various hormones and consequently affecting social well-being and quality of life in cities. Objectives: This study aimed to investigate the effect of noise pollution on the levels of testosterone, thyroid, and cortisol hormones in male rats. Methods: In this experimental study, a total of 70 male Wistar rats (200 - 250 g) were randomly assigned into one control and six experimental groups, with 10 rats in each group. Experimental groups were exposed to noise with different intensity (dB) and time (min) as follows: (I) 60 dB, 30 min; (II) 60 dB, 60 min; (III) 85 dB, 30 min; (IV) 85 dB, 60 min; (V) 110 dB, 30 min; (VI) 110 dB, 60 min; (VII) controls. Animals in the experimental groups were exposed to noise in an acoustic chamber designed for this purpose for 50 days. The Noise.exe software was used to generate noise, and the sound level meter (model TES 1358) was used to determine the accuracy of the intensity and frequency of sound. To determine plasma levels of the hormones, appropriate research and commercial kits were used, which were based on the ELISA method. To determine the concentration of hormones other than TSH, human assay kits were used. All statistical tests were performed in SPSS software version 21. Results: Serum levels of cortisol in the 110-dB (30 and 60 min), 65-dB (60 min), and 85-dB (60 min) groups were significantly higher than the control group (P ≤ 0.05). Also, cortisol levels in the 65-dB and 85-dB (30 minutes) groups were higher than the control group; however, the increase was not significant (P > 0.05). The levels of T4, T3, and TSH in the 60-dB and 85-dB groups were significantly lower than in the control group (P ≤ 0.05). The serum levels of T4, T3, and TSH hormones in the 110-dB group were insignificantly lower than the control group (P > 0.05). The serum level of testosterone in the 110-dB group was significantly lower than the control group (P ≤ 0.05). The mean serum levels of testosterone in the 65-dB and 85-dB groups were insignificantly lower than the control group (P > 0.05). Conclusions: Based on this study, exposure to noise pollution increased cortisol secretion and decreased T4, T3, TSH, and testosterone levels in rats. As this hormonal imbalance may create direct and indirect effects, studies and strategies are recommended to control the imbalance of hormones in the polluted environments.

scholarly journals Melatonin menurunkan berat badan tetapi tidak menurunkan kadar TNF-α pada tikus wistar jantan yang diberi minyak jelantah selama 28 hari

2017 ◽  
Vol 5 (2) ◽  
pp. 127-132
Author(s):  
Anugrah Novianti ◽  
Edi Dharmana ◽  
Nyoman Suci Widyastiti
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Treated Group ◽  
Waste Cooking Oil ◽  
The Body ◽  
Control Group ◽  
Cooking Oil ◽  
Tnf Α ◽  
Significant Difference ◽  
Male Wistar Rats

Backgound: Non Alcoholic Fatty Liver Disease (NAFLD) occurs when the intake and free fatty acid synthesis occurs more frequently than its oxidation and resecretion in the blood. Melatonin is a powerful antioxidant that can boost the synthesis of endogenous antioxidants in the body, suppress the inflammatory response and inhibit the formation of steatosis.Objective: To analyze the effect of melatonin supplementation in reducing body weight andTNF-α levels in male Wistar rats were fed by waste cooking oil.Methods: True experimental studyusing post-test only control group design. This study was done on 18 male wistar rats were divided into 3 groups : the positive control group (P0) was administrated waste cooking oil, the treated group 1 (P1) was administrated waste cooking oil and 5mg/kgBW melatonin, and the treated group 2 (P2) was administrated waste cooking oil and 10mg/kgBW melatonin for 28 days.Data analysis using One Way ANOVA test and followed by Tukey test to determine the most effective dose of melatonin.Results: There was significant difference in body weightbetween P2group and K0 group (p=0,019) with the mean body weight difference was 19,167g lower than K0 group. There was no difference in TNF-α levels between the three groups (p=0,155). Conclusion: Melatonin dose of 10mg/kgBWloses body weight male Wistar rats have given by waste cooking oil for 28 days, but does not reduce TNF-α levels.

Melatonin modulates 900 MHz microwave-induced lipid peroxidation changes in rat brain

2006 ◽  
Vol 22 (5) ◽  
pp. 211-216 ◽  
Author(s):  
Halis Köylü ◽  
Hakan Mollaoglu ◽  
Fehmi Ozguner ◽  
Mustafa Nazýroölu ◽  
Namýk Delibap
Keyword(s):  
Lipid Peroxidation ◽  
Brain Cortex ◽  
Treated Group ◽  
Male Rats ◽  
Control Group ◽  
Protective Effects ◽  
Sprague Dawley ◽  
Melatonin Administration ◽  
Anti Oxidant ◽  
The Brain

Microwaves (MW) from cellular phones may affect biological systems by increasing free radicals, which may enhance lipid peroxidation levels of the brain, thus leading to oxidative damage. Melatonin is synthesized in and secreted by the pineal gland at night and exhibits anti-oxidant properties. Several studies suggest that supplementation with anti-oxidant can influence MW-induced brain damage. The present study was designed to determine the effects of MW on the brain lipid peroxidation system, and the possible protective effects of melatonin on brain degeneration induced by MW. Twenty-eight Sprague-Dawley male rats were randomly divided into three groups as follows: (1) sham-operated control group (N-8); (2) study 900-MHz MW-exposed group (N-8); and (3) 900-MHz MW-exposed-melatonin (100 mg/kg sc before daily MW exposure treated group) (N-10). Cortex brain and hippocampus tissues were removed to study the levels of lipid peroxidation as malonyl dialdehyde. The levels of lipid peroxidation in the brain cortex and hippocampus increased in the MW group compared with the control group, although the levels in the hippocampus were decreased by MW-melatonin administration. The brain cortex lipid peroxidation levels were unaffected by melatonin treatment. We conclude that melatonin may prevent MW-induced oxidative changes in the hippocampus by strengthening the anti-oxidant defense system, by reducing oxidative stress products.

scholarly journals Nephroprotective effect of Aloe barbadensis Miller against gentamicin induced nephrotoxicity in long evans male rats

2021 ◽  
Vol 16 (1) ◽  
pp. 33-38
Author(s):  
Farjana Rahman ◽  
Mahmuda Begum ◽  
Rama Chowdhury
Keyword(s):  
Body Weight ◽  
Aloe Vera ◽  
Basal Diet ◽  
Serum Levels ◽  
Treated Group ◽  
Male Rats ◽  
Control Group ◽  
Long Evans ◽  
Nephroprotective Effect ◽  
Experimental Group

Background: Kidney is an essential excretory organ of our body. It can be damaged by poisonous effect of chemicals, toxin, prolonged and uncontrolled use of drugs. Aloe vera is a herbal plant, it can be used for the prevention and treatment of kidney damage due to its natural compatability. Objective: To observe the nephroprotective effect of Aloe vera against gentamicin induced nephrotoxicity in long evans male rats. Method: This experimental study was conducted in the Department of Physiology, Sir Salimullah Medical College (SSMC), Dhaka from 1st January 2018 to 30th December 2018. For this, forty five (45) apparently healthy long evans male rats, 90-120 days old, weighing between 150-200 g were taken and divided into control group (group A) and experimental group (group B - Aloe vera pretreated and gentamicin treated group). Control group was subdivided into group A1 (baseline control group) and group A2 (gentamicin treated control group). Each group consisted of fifteen (15) rats. At the beginning of the study period initial body weight of rats were measured at day 01. All the rats received basal diet for thirty (30) days. Group A1 received only basal diet for 30 consecutive days (started from day 1 to day 30). In addition to basal diet, group A2 received injection gentamicin intraperitoneally once daily in the morning (80 mg/kg/day) for last 5 days (26th to 30th) of the study period. Again, in addition to basal diet, experimental group received Aloe vera orally (200 mg/kg/day) for 30 days and injection gentamicin intraperitoneally (80 mg/kg/day) for last 5 days (26th to 30th). After measuring final body weight all the rats were sacrificed on day 31. Blood sample was collected from heart. Serum levels of creatinine and urea were estimated for assessment of kidney function. Statistical analysis was done by one way ANOVA test followed by post hoc-Bonferroni test. Result: Mean serum levels of creatinine and urea were significantly (p<0.001) higher in gentamicin treated control group in comparison to that of base line control group. Again these levels were significantly (p<0.001) lower in Aloe vera pretreated and gentamicin treated group than that of gentamicin treated control group. Conclusion: From this study, it is concluded that Aloe vera has nephroprotective effect against gentamicin induced nephrotoxicity. J Bngladesh Soc Physiol 2021;16(1): 33-38

scholarly journals Biochemical Effects of Oleuropein in Streptozotocin Induced Diabetic Male Rat

2018 ◽  
Vol 41 (2) ◽  
pp. 187-194
Author(s):  
Sina Amirnahavandirahbar ◽  
Mohammad Reza Nasirzadeh
Keyword(s):  
Blood Glucose ◽  
Treatment Group ◽  
Serum Levels ◽  
Diabetic Group ◽  
Atherogenic Index ◽  
Male Rats ◽  
Male Rat ◽  
Control Group ◽  
Biochemical Effects ◽  
Male Wistar Rats

Abstract Diabetes is a chronic disease characterized by a disorder in the metabolism of proteins, fats, and carbohydrates. The liver as a non-insulin dependent organ plays an important role in the regulation of blood fat and glucose. Most blood glucose lowering drugs that are introduced for treatment have side effects in long-term consumption. Therefore, to control diabetes and its complications, the use of herbal drugs is widely considered nowadays. The present study investigates the biochemical effects of oleuropein in diabetic male rats. In this study, 30 adult male Wistar rats with a weight range of 190±30 gr were equally divided into 3 groups randomly: 1) control group or intact animals, 2) diabetic animals, and 3) treatment group, which received 60 mg/kg oleuropein for 30 days by gastric gavage. Diabetes was induced in diabetic and treatment groups by injection of streptozotocin (60 mg/kg) intraperitoneally. At the end of the treatment, the levels of triglyceride, cholesterol, LDL, HDL, VLDL, blood glucose, HbA1C, and activity of AST and ALT were determined. The results showed that the serum lipid profile and blood glucose increased significantly in the diabetic group compared with the control group (p<0.05). Also, HbA1C and atherogenic index decreased significantly in the treatment group compared with the diabetic group (p<0.05). This study showed that oral administration of oleuropein has hypoglycemic effects, which can reduce the serum levels of the lipids profile and the atherogenic index in streptozotocin-induced diabetic male rats.

scholarly journals Silymarin mitigates diclofenac-induced liver toxicity through inhibition of inflammation and oxidative stress in male rats

2019 ◽  
Vol 8 (3) ◽  
pp. 231-237 ◽  
Author(s):  
Pantea Ramezannezhad ◽  
Ali Nouri ◽  
Esfandiar Heidarian
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Total Bilirubin ◽  
Liver Toxicity ◽  
Treated Group ◽  
Male Rats ◽  
Control Group ◽  
Tnf Α ◽  
Ameliorative Effect ◽  
And Oxidative Stress

Introduction: Diclofenac (DIC) is one of the compounds derived from acetic acid which isknown for its anti-inflammatory and analgesic attributes. Silymarin is a flavonoid compoundwhich is derivate from Silybum marianum seeds. This research was done to assess the protectiverole of silymarin against liver toxicity induced by DIC in male rats.Methods: Randomly, 40 male Wistar rats were assigned into five groups as follows: Group 1:control group, Group 2: DIC-only treated (50 mg/kg, i.p), Group 3: silymarin-only treated (200mg/kg, p.o); Groups 4 and 5: DIC (50 mg/kg, i.p) plus silymarin (100 mg/kg and 200 mg/kg, p.o,respectively) treated. Various biochemical, molecular, and histological parameters were evaluatedin serum and tissue.Results: In the DIC-only treated group, the levels of liver glutathione peroxidase (GPx), superoxidedismutase (SOD), intracellular glutathione (GSH) and catalase (CAT) significantly diminished andthe levels of total bilirubin, alkaline phosphatase (ALP), nitrite, alanine aminotransferase (ALT),malondialdehyde (MDA), serum tumor necrosis factor-α (TNF-α), aspartate aminotransferase(AST), and TNF-α gene expression were remarkably elevated relative to control animals. In otherhands, treatment with silymarin caused a noticeable elevation in GPx, SOD, GSH, CAT and aremarkable reduction in levels of total bilirubin, ALP, nitrite content, ALT, MDA, serum TNF-α,AST and TNF-α gene expression relative to DIC-only treated group. Histopathological injurieswere also improved by silymarin administration.Conclusion: The results confirm that silymarin has an ameliorative effect on liver toxicity inducedby DIC and oxidative stress in male rats.

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