scholarly journals Examination of Impact of Arsenic Stress on Soil Microbes

2020 ◽  
Vol 21 (2) ◽  
pp. 342-348
Author(s):  
Rashida Sultana

The research was performed at Botany department, Nusrat Jahan College Rabwah Pakistan to screen stress tolerance level of soil microbes (pseudomonas and penicillium) taken from NARC (National Agricultural Research Centre) Pakistan against different levels of sodium arsenite stress (1 mg/L to 10 mg/L). The research was based on completely randomized design. Three drops were applied to microbes grown on CLED (cysteine-, lactose-, and electrolyte-deficient) media to determine zone of inhibition through disk, well and spread method, while control group was without sodium arsenite application. After application of various levels of arsenic stress organisms were incubated for 24 h at 37°C. After incubation, zones of inhibition were measured. Our study has shown that both micobes cannot overcome higher levels of arsenic stress because in higher stress petriplates, increased inhibitory zones were observed.

2021 ◽  
Vol 3 (3) ◽  
pp. 56-61
Author(s):  
Sharmila Rani Mallick ◽  
AKM Quamruzzaman ◽  
Md. Altaf Hossain ◽  
M. Mizanur Rahman ◽  
Md. Azizul Hoque ◽  
...  

A study based on completely randomized design with three replications was carried out at Tuber Crops Research Centre (TCRC), Bangladesh Agricultural Research Institute (BARI), Gazipur with a view to evaluating and classifying agro-morphological traits in 12 potato varieties to enumerating the genetic diversity. Discriminant function analysis (DFA) was done to determine the set of discriminatory functions contributed to separating 12 potato varieties into four distinct clusters. Discriminant function analysis showed that, function 1 explained 78.21% and function 2 explained 21.79% of total variance. The first function was more related to leaf area (having coefficient value of 0.661) and second function was more related to minituber per plant and plant height (having coefficient value of 0.644 and 0.523, respectively). Among 4 clusters, cluster II showed the highest distance (29.79) with cluster III followed by the distance between clusters III and IV (24.70) so the varieties in these clusters were genetically diverse. Based on mean value of the six quantitative characters for each of the four clusters and the inter cluster distance the cluster III and IV might be selected for future breeding program as a better parent to get positive heterosis. Cluster III contained six varieties BARI Alu 29, BARI Alu 35, BARI Alu 36, BARI Alu 37, BARI Alu 7 (Diamant), BARI Alu 8 (Cardinal) and which were characterized by lowest plant height and average values for other characters. Cluster IV contained three potato varieties BARI Alu 41, BARI Alu 46 and BARI Alu 53 which was characterized by highest plant height and second highest leaf area.


2018 ◽  
Vol 12 (2) ◽  
pp. 103-109
Author(s):  
Feri Hardiansah Manihuruk ◽  
Ismail Ismail ◽  
Rastina Rastina ◽  
Razali Razali ◽  
Mustafa Sabri ◽  
...  

This study aimed to determine the effect of fermented kelor leaves (Moringa oleifera) flour as additive in animal feed to increase the weight of boiler carcass. The samples used were 28 DOC strain Cobb CP-707 aged 1 day. This study was conducted using one way pattern complete randomized design with 4 treatments and 7 replications. Control group (P0) was fed with commercial feed; P1 was given 5% fermented kelor leaves flour;  P2 was given 7,5% fermented kelor leaves flour; and P3 was given 10% concentration of fermented kelor leaves flour. Feed replacement was conducted on 08.00 am and 5.00 pm everyday, and water was given ad libitum. Chemical content of flour before and after fermented was analyzed at Research Centre of Biotechnology and Biological Resources, IPB. Body weight measurement was carried out at Veterinary Public Health Laboratorium. Data were analyzed using ANOVA with SPSS 16.0. Mean value (±SD) of carcass weight for P0, P1, P2, and P3 were 938,71±180,83, 1011,86±101,64, 1010,71±126,69, and 907,43±190,35, respectively. This study showed that adddition of fermented kelor leaves flour for 21 days did not affect (P0,05) the weight of broilers carcass.


Food Biology ◽  
1970 ◽  
pp. 13-15
Author(s):  
Shariq Mahmood Alam ◽  
Imdad Ali Mahmood, Muhammad Arshad Ullah ◽  
Taj Naseeb, Nausherwan N. Nawab ◽  
Syed Ishtiaq Haider ◽  
Syeda Sana Aamir

A pot experiment was conducted to investigate the effect of Zn and Fe application on growth and yield of garlic (Allium sativum L.) var. Desi Sufaid at National Agricultural Research Centre, Islamabad during Rabi season 2018-2019. Eight kilogram of sandy clay loam soil was filled in the pots. Four garlic cloves were sown in each pot. The treatments of Zn, Fe and their mixture (1:1 ratio) @ 5 kg ha-1 along with basal dose of N, P and K (100, 75 and 50 mg kg-1) fertilizer were applied at sowing following complete randomized design with three replications. The plants were allowed to stand till maturity and data on growth traits like plant height (cm), pseudo stem diameter (cm), leaf length (cm), leaves plant-1, bulb diameter (cm), cloves (bulb-1), clove weight (g) and bulb yield (g plant-1) were recorded at harvest. The mean data were analyzed statistically. The highest growth attributes like plant height, number of leaves per plant, bulb diameter, cloves, clove weight and bulb yield were harvested with micronutrients (Zn and Fe) along with basal dose of N, P and K (100, 75 and 50 mg kg-1) application which were significantly higher as compared to control. Though, all the treatments showed positive effect through growth characteristics and yield, however, the mixture of Zn and Fe application produced comparable garlic yield among all the treatments which was 6 and 4 (%) higher than that of sole application of Zn and Fe, respectively. Significant improvement in Zn and Fe concentration in garlic plant tissues was observed with the application of Zn and Fe respectively. Overall the combination of both micronutrients performed better than rest of the treatments through improved growth traits and bulb yield.


2021 ◽  
pp. 1-9
Author(s):  
Badar-uz- Zaman ◽  
Zaryab Gul ◽  
Muhammad Suhaib ◽  
Muhammad Ishaq ◽  
Haris Khurshid

Aims: To evaluate the effect of K2SO42- application on growth of soybean cultivars besides chemical parameters under salt stress. Study Design: Laid out the experiment in Complete Randomized Design in triplicates.  Analyzed the data statistically by using the statistical software Statistix 8.1. Place and Duration of Study: The study was conducted in Soil Salinity and Bio-saline Research green house and in the laboratory of Land Resources Research Institute at National Agricultural Research Centre, Islamabad (under PARC), Pakistan for three months. Methodology: To study germination parameters under salt stress, NaCl was used @ 0, 100, 120 and 140 mmol L-1. At seedling stage, interactive effect among salt stress (0 and 4.5 dSm-1), potassium sulphate (KS) application @ 10 mmol L-1 and soybean (Cvs. NIBGE-301 and NIGBE-158) was recorded for growth and ion relations.  Results: Germination, biomass, bio vigor, mass vigor and sap vigor of the seedlings and ion relations were affected highly significantly (p≤ 0.01).  Under interactive effect of KS and salt stress at vegetative stage, Na+/K+ in the plants declined with KS application. Sulphur and phosphorus concentration in the plants changed under the same conditions. Conclusion: Induced salt stress revealed germination, seed bio vigor, seeding mass vigor, and seedling sap vigor seedling and other vegetative parameters of soybean cultivars. Potassium sulphate application revealed genotypes differential response to ion relations and growth under salt stress. NIBGE-301 was more tolerant to salt stress and more responsive to potassium sulphate application than NIGBE-158.


2019 ◽  
Vol 36 ◽  
pp. 148-156
Author(s):  
Md. H. Azad ◽  
M. R. Tiwari ◽  
N. R. Poudel ◽  
B. M. Shah ◽  
B. K. J.C ◽  
...  

An experiment was conducted at Regional Agricultural Research Station, Parwanipur, Bara for 38 days to test the effect of Khesari leaves inclusion in broiler feed and its effect on growth performance. Altogether 135 day-olds Cobb-500 broiler birds were procured from Shivam Hatchery, Birgung and divided into 3 treatments with 3 replications (15 birds in each replication) by using completely randomized design. Concentrate feed was purchased from Posak Feed industry, Birgung. Control group (T1) was fed without inclusion of Khesari leaves and whereas T2 and T3 groups were fed 5% and 10 % Khesari leaves added feed respectively. Diet replaced with 5 % and 10 % Khesari leaves were offered from 14th day of trial to T2 and T3 broilers group respectively. Experimental birds were provided adlib amount of grower feed (B1) for 21 days and that after finisher feed (B3) for 21 days and had easily access to drinking water. Feed intake was recorded daily and body weight gain was measured in 7 days interval. Experiment revealed that highest weight gain was observed in T3 (2.55 kg) followed by T1 (2.48) where inclusion of 10 % Khesari leaves and normal feed respectively, which was also significant among diet groups. Similarly, highest cumulative feed intake was also observed for T3 (4.23 kg) followed by T1 (3.90 kg) which was also significant among diet groups. Experiment suggested that further study should be carried out to precise the appropriate level of organic acids inclusion and higher cost benefit ratio.


2021 ◽  
pp. 23-29
Author(s):  
Md. Riazul Islam ◽  
Md. Nazmul Hasan Mehedi ◽  
Rumman Ara ◽  
Abu Jafor Mohammad Obaidullah ◽  
Md. Moniruzzaman ◽  
...  

Genetic diversity is the base for survival of plants in nature and for crop improvement. Diversity in plant genetic resources provides opportunity for plant breeders to develop new and improved cultivars with desirable characteristics. Therefore, an experiment was employed to determine the genetic variability and evaluate the growth performing parameters of different Chaba germplasm at the research field of Regional Spices Research Centre, Bangladesh Agricultural Research Institute, Magura during kharif 1 season in 2018-19 following completely randomized design with three replications.  Six germplasm namely PCmag-001, PCmag-002, PCmag-003, PCmag-004, PCmag-005 and PCmag-006 were collected from khulna region of Bangladesh and transplanted adjunct to different tree species mainly mango. Among the six germplasm heighest plant height (450 cm), number of branch (35), number of leaves (350), internodes length (16 cm) and vine diameter (2.5 cm) after one year of planting was recorded from PCmag-001 germplasm followed by PCmag-002 while the lowest plant height (450 cm), number of branch (35), number of leaves (350), internodes length (16 cm) and vine diameter (2.5 cm) were found from the germplasm PCmag-005. Through the observation of one year field trail in terms of growing characteristics the germplasm PCmag-001 and PCmag-002 can be chosen for future breeding material to release a commercial variety in southern part of Bangladesh.


2017 ◽  
Vol 5 (2) ◽  
pp. 93-96
Author(s):  
Shashi Kant Shukla ◽  
◽  
Awadhesh Kumar ◽  
Anupam Dikshit ◽  
◽  
...  

The present study aims the impact of Pseudomonas putida on different growth parameters of Trigonella sp., a leguminous plant to support the requirement of food, protein along with their medicinal value in the rural areas of India. A pot experiment was arranged based on completely randomized design with four replications at Biological Product Laboratory, Botany Department, University of Allahabad. Treatments were given at the seed level with one of control. Results indicated that application of P. putida significantly improved vegetative growth and showed an edge on the growth of the fenugreek as compared to the control.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 158-159
Author(s):  
Hailey Wooten ◽  
Hwanhee Kim ◽  
Amanda Rakhshandeh ◽  
Anoosh Rakhshandeh

Abstract We previously found that i.m. injection of GRA, similar to in-feed antibiotics, improves measures of intestinal integrity, nutrient digestibility, and overall growth performance in nursery pigs. In addition, we have demonstrated that the mitigating effects of GRA on the stress of weaning is predominantly done by subsiding the inflammatory response that often accompanies early weaning. The objective of the current study was to explore the most practical methods for delivering GRA to newly weaned pigs. One-hundred and sixty-seven PIC pigs were weaned at 25 ±1.0 days of age (BW 7 ±1.2 kg), and assigned to fourteen treatments (3-4 pigs /pen; 3 pens/treatment). Seven treatments were tested within two sexes (gilts vs. barrows). The treatments were: 1) i.m. injection of GRA (two injections, 0.2 mg/kg BW at -1 and 3 d post-weaning), 2) two levels of in-water GRA (0.8 and 1.6 ppm), 3) in-feed GRA (2.5 and 5.0 ppm), 4) in-feed antibiotic (ANT, 100 ppm tylosin), and 5) control. Dexamethasone was used as GRA. Pigs had free access to in-feed or in-water GRA or ANT during the 1st-week post-weaning. Parameters of growth performance were measured weekly until the end of the nursery phase. A completely randomized design and repeated measurement ANOVA (PROC MIXED) in SAS were used for statistical analyses. In-water, in-feed, and i.m. GRA treatments resulted in growth performance outcomes comparable to that of in-feed ANT. Relative to CON pigs, both ANT and GRA improved ADG and G: F during the nursery phase (P < 0.05). Among the delivery methods tested, 2.5 ppm of in-feed GRA resulted in superior ADG (GRA vs. CON; 0.29 vs. 0.20 kg/d) and G: F (0.63 vs. 0.40 ± 0.05), compared to the control group (P < 0.02). Collectively, these results suggested that in-feed treatment is the best method for delivering GRA to newly weaned pigs.


1993 ◽  
Vol 121 (2) ◽  
pp. 193-198 ◽  
Author(s):  
Z. M. Sawan ◽  
M. H. Mahmoud ◽  
B. R. Gregg

SUMMARYA decline in supplies of organic manures and the increased use of chemical fertilizers has led to a decrease in the micronutrient content of the soil, with attendant effects on yield.Field studies were conducted in 1986 and 1987 at the Giza Agricultural Research Centre, Egypt, to determine the effect of Cu-EDTA and Mn-EDTA foliar sprays on growth, mineral content, yield components and fibre properties of the Egyptian cotton cultivar Giza 75 (Gossypium barbadense L.).All combinations used significantly increased the uptake of Cu and Mn and the dry matter yield. The earliness of harvest and the value of yield components were all increased by the application of Cu or Mn; 25 mg/1 of both Cu and Mn gave the highest values. Lint percentage and fibre properties were not significantly affected.This study indicated that the yield of cotton, grown under otherwise standard conditions, may be increased by foliar sprays of Cu-EDTA and Mn-EDTA combined.


2015 ◽  
Vol 39 (4) ◽  
pp. 641-659
Author(s):  
MAHS Jahan ◽  
MAR Sarkar ◽  
NCD Barma ◽  
MNA Mondal ◽  
MNS Ferdousi

A field experiment was conducted at Regional Wheat Research Centre of the Bangladesh Agricultural Research Institute, Joydebpur, Gazipur for 2 consecutive years during 2006-07 and 2007-08. The objectives were to find out the optimum nutrient management practice on tuber yield, nutrient balance and economics of potato cultivation. Twelve nutrient management treatments were tested in a randomized complete block design with 3 replications. Treatments were T1=HYG (0-198-44-194-24-6-1.2), T2=MYG (0-140-34-138-18-4.5-0.9), T3=IPNS (10000-168-38-170-18-6-1.2), T4=STB (0-171-40-164-22-5-1), T5=FP (0-97-16-91-0-0-0), T6=CON (0-0-0-0-0-0-0), T7=HYG+CRI, T8=MYG+CRI, T9=IPNS+CRI, T10=STB+CRI, T11=FP+CRI, T12=CON+CRI kg ha-1 CDNPKSZnB for potato. The highest tuber yield of potato was obtained from STB+CRI (27.64 t ha-1) followed by IPNS+CRI (27.35 t ha-1), STB (27.10 t ha-1), IPNS (26.83 t ha-1), HYG+CRI (26.52 t ha-1) and HYG (26.10 t ha-1) in 2006- 07. Similar trend was found in 2007-08. The highest tuber yield and yield contributing parameters were noticed in CRI plots than without CRI. Except N, remaining nutrient balance like P K S Zn and B were found positive in case of HYG, MYG, IPNS and STB along with or without CRI nutrient managements. The balance was found almost negative in case of FP and CON. The highest BCR was observed in STB+CRI (3.96) followed by STB (3.93), IPNS+CRI (3.76), and IPNS (3.74). The higher gross return (Tk. 283331 ha-1) and also gross margin (Tk. 211761 ha-1) was observed from the same treatment. DOI: http://dx.doi.org/10.3329/bjar.v39i4.22544 Bangladesh J. Agril. Res. 39(4): 641-659, December 2014


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