scholarly journals Pharmacognostic and Preliminary Phytochemical Investigation of Corchorus Fascicularis LAM. Stems

Author(s):  
Sanjay B Sonawale

Abstract: Stems of plant Corchorus fascicularis L. are reported to possess medicinal values in traditional system of medicine. The present investigation deals with preliminary phytochemical investigation of Stems of Corchorus fascicularis L. which includes physicochemical parameters like ash values, extractive values and moisture content. Phytochemical investigation of nhexane, chloroform, ethanol and water extract revealed the presence of glycosides, tannins, terpenoids, steroids, carbohydrates, alkaloids, saponins and proteins. The main aim of present investigation is to study the pharmacognostic characters and phytochemical standard of Stems of Corchorus fascicularis L. which could be used to prepare a monograph for the proper identification of plant. Keywords: Stems, Corchorus fascicularis L., Physicochemical, Phytochemical analysis.

2021 ◽  
Vol 12 (3) ◽  
pp. 631-636
Author(s):  
Disha Prakash Prajapati ◽  
Tanvi R Dodiya

Aims: To evaluate pharmacognostic and preliminary phytochemical analysis of stem of Conocarpus lancifolius including macroscopic, microscopic and physicochemical parameters. Methods and Material: Macroscopic and microscopic properties of dried stem were investigated. Preliminary phytochemical analysis of various extracts and fluorescence analysis of dried stem powder was performed using various chemicals. Physicochemical parameters were evaluated as per WHO guidelines. Results: Organoleptic parameters of stem of C. lancifolius like colour, shape, size etc were noted.  Transverse section of fresh stem revealed the presence of epidermis, cortex, sone cells, phloem, xylem and pith. Whereas, powder microscopy showed the presence of epidermis, xylem, stone cells, phloem fibre with crystals of calcium oxalate etc. Phytochemical screening of methanolic extract of stem showed presence of alkaloids, flavonoids, glycoside, steroids etc. Ethyl acetate extract showed the presence of alkaloids and steroids while aqueous extract showed presence of tannins. Physicochemical parameters viz. ash value, extractive value, moisture content, fluorescence analysis and foaming index of stem powder were determined. The total tannins were also determined in the stem of C. lancifolius. Conclusion: The present study revealed pharmacognostic and preliminary phytochemical parameters of stem of C. lancifolius which would be beneficial for its standardization and future scope of the work.


Author(s):  
Dahiya P

  Objective: The inhibitory properties of successive extracts from Dioscorea bulbifera (Dioscoreaceae) tubers have been evaluated for the presence of phytochemical constituents and antimicrobial efficacy against multidrug-resistant (MDR) clinical isolates was evaluated.Methods: The tuber of D. bulbifera was oven dried and extracted successively with n-hexane, chloroform, methanol, ethanol, and water. The antimicrobial potential of successive extracts against MDR isolates was studied by agar well-diffusion method. Qualitative phytochemical analysis was performed.Results: Qualitative phytochemical analysis demonstrated the presence of steroids, flavonoids, cardiac glycosides, saponins, and reducing sugars in almost all the extracts tested. Anthraquinones, phlobatanins, and tannins were not reported in any extracts tested. The in vitro antimicrobial activity of various solvents and water extracts of D. bulbifera was further investigated against ten MDR bacteria and three fungi, respectively. Aqueous and chloroform extracts were found to be more potent being capable of exerting significant inhibitory activities against the majority of the isolates such as Escherichia coli, Acinetobacter sp., Salmonella paratyphi, Klebsiella pneumoniae, and Candida albicans. The highest inhibitory activity was observed for K. pneumoniae with wide inhibition zone diameters (17 ± 0.15 mm), followed by E. coli 1(13 ± 0.11) mm, and Acinetobacter sp. (11 ± 0.12).Conclusion: Based on the present study, the extracts of D. bulbifera tubers have shown excellent activity against MDR microbial cultures tested. Further study is recommended for clinical evaluation, of the efficacy of crude extract in herbal medicine that can serve as a base for the development of novel potent drugs and phytomedicines.


2014 ◽  
Vol 1 (2) ◽  
pp. 105-108
Author(s):  
Sharmila S ◽  
Kalaichelvi K ◽  
Jayanthi G

Medicinal plants are an integral component of alternative medical care. For millennia, Indian people traditionally played an important role in the management of biological resources and were custodians of related knowledge that they acquired through trial and error over centuries. India has a rich wealth ofmedicinal plants and the potential to accept the challenge to meet the global demand for them. In recent time, focus on plant research has increased all over the world and a large body evidence has collected to show immense potential of medicinal plants used in various traditional system. Plumbago zeylanica L.(Plumbaginaceae) is a tropical plant and the source for the well known ayurvedic drug ‘chitrakah’. Different parts of this plant are widely used for variety of medicinal treatments. Especially used for appetite, indigestion, piles, worms, liver diseases and cancer treatments. The present study was therefore carried outto provide pharmacognostic details of root of Plumbago zeylanica. The physico-chemical analysis of ash content indicated the presence of inorganic matter and siliceous matter in the drug. The phytochemical analysis indicated the presence of alkaloids, phenols, flavonoids, tannins and terpenoids. Plumbagin is anaturally occurring yellow pigment, produced by member of plumbaginaceae that can be obtained from roots


Author(s):  
K.P. Jaiganesh ◽  
T.J. Jasna ◽  
A.C. Tangavelou

Hugonia mystax L., (Linaceae), is commonly distributed in the thorny scrubs and tropical dry evergreen forests of Tamil Nadu, which has been valued for centuries in traditional system of medicine for the treatment of various ailments. In the present study was an attempt to investigate the phytochemical nature and anti-inflammatory, antimicrobial potential by adopting suitable methods. Phytochemical analysis of Hugonia mystax L., plant extracts revealed the presence of various biochemical compounds such as alkaloids, flavonoids, glycosides, triterpenoids and saponins etc. Since triterpenoids and flavonoids have remarkable anti-inflammatory activity, so our present work aims at evaluating in vitro anti inflammatory activity of Hugonia mystax L., by HRBC membrane stabilization method. The inhibition of hypotonicity induced HRBC membrane lysis was taken as a measure of the anti-inflammatory activity. The percentage of membrane stabilization for ethanolic extracts and Diclofenac sodium were done at different concentrations. The maximum membrane stabilization of Hugonia mystax L., extracts was found to be 94.97 % at a dose of 2000 μg/ml. Therefore, our studies support the isolation and the use of active constituents from Hugonia mystax L., in treating inflammations.


Author(s):  
S. ANNAI THERASA ◽  
G. SOBIYA ◽  
S. MABEL PARIMALA

Objective: Andrographis paniculata (Family: Acanthaceae) is a well-known medicinal plant used in the Indian traditional system of medicine for the treatment of many chronic diseases. The present study was aimed to quantify secondary metabolites, determine antioxidant, and anticancer activity of ethanol extract of A. paniculata leaves. Methods: Leaf sample was macerated with ethanol solvent. Alkaloids, terpenoids, saponins, phenols, and flavonoids were quantified with standard calibrations. The antioxidant potential was tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and ferric reducing antioxidant power (FRAP) assays. In vitro anticancer activity was evaluated using human epithelial type 2 (HEp-2) cell line. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to estimate the cytotoxicity of the extracts. Apoptotic and necrotic effects were characterized by DNA fragmentation assay and fluorescence microscopy using the dual acridine orange/ethidium bromide (AO/EB) staining method. Results: The phytochemical analysis reveals the presence of alkaloids, saponins, phenols, flavonoids, terpenoids, and steroids. Alkaloids, terpenoids, saponins, phenol, and flavonoid content were recorded as follows: 9.84%, 8.42%, 13.94%, 44.37 mg gallic acid equivalent/100 g, and 904 mg quercetin equivalent/100 g, respectively. The antioxidant activity from DPPH, ABTS, and FRAP assays showed dose-dependent inhibition of free radicals. In cell viability tests, cell death with increasing extract concentration was observed. DNA fragmentation and AO/EB stain confirmed apoptosis and necrosis in extract-treated cells. Conclusion: The results indicate that A. paniculata is a promising source for the development of antioxidant and anticancer drugs.


2018 ◽  
Vol 879 ◽  
pp. 101-107
Author(s):  
Udomdeja Polyium ◽  
Na Thaisong Phinthida

The biodiversity of local plants on Phraek Nam Daeng community Amphawa, Samut Songkhram province, Thailand has been the result of the terrain as a source of freshwater and salty. This study was carried out to evaluate the phytochemical screening and nutritional value of 6 local plants in the Phraek Nam Daeng community including: Azima sarmentosa Benth, Pluchea indica (L) Less,, Boehravia diffusa L, Acanthus ilicifolius L, Sesuvium portulacastrum L and Derris trifoliata Lour. The phytochemical analysis was performed for alkaloid, tannin, terpenoid, flavonoid, anthraquinone and cardiac glycoside. Nutritional analysis of proximal composition of local plants using the method of AOAC (2005),Which consist of moisture content, protein content, lipid content, fiber content, total ash content and carbohydrate content. The results showed that phytochemical screening of 6 local plants, the methanolic extracts have the presence of bioactive substances such as alkaloid, tannin, terpenoid, flavonoid, anthraquinone and cardiac glycoside. The most nutritional composition of local plants showed that the moisture content was Sesuvium portulacastrum L, protein content was Acanthus ilicifolius L, lipid and carbohydrate content was Azima sarmentosa Benth. & Hook, fiber content was Pluchea indica (L) Less., these represent the potential of food crops for food security and sustainable use of biodiversity.


2016 ◽  
Vol 2 (1) ◽  
pp. 01-10 ◽  
Author(s):  
Anju Mathew ◽  
A Malar Retna

Scoparia dulcis Linn has been widely reported to have pharmacological uses arising from its wide spread uses. The different extracts were prepared by successive extraction with petroleum ether, chloroform, ethanol and water using soxhlet distillation method. Phytochemical analysis of plant extract revealed the presence of alkaloids, carbohydrates, glycoside, tannins, starch etc.Thin layer chromatography and Gas chromatography-mass spectrometric analysis revealed the presence of different components in the plant extract. Among the 50 components obtained, 10 important organic compounds were analyzed. All these compounds are found to be having some medicinal application.UV visible spectroscopic analysis of extract of Scoparia dulcis reported four chromatogram figures which showed prominent peaks having maximum absorption of 666 nm corresponded to wavelength of methylene blue and brilliant blue. FTIR spectroscopic analysis reveals the presence of important functional groups like-OH,-NO2,-SO3,-SH, -COOH, NH2, R-X etc. Antibacterial activity of petroleum ether, chloroform, and ethanol and water extracts of stems and leaves of Scoparia dulcis reveals that chloroform and ethanol extracts shows maximum resistance against Staphylococcus while ethanol and aqueous extracts showed maximum resistance against Klebsiela pneumonia.The exciting fact came out of the study is that water extract of Scoparia dulcis showed great potential to dissolve the Calcium oxalate crystals ie, the plant extract shows invitro antilithiatic activity for kidney stones. Thus Scoparia dulcis act as a source of different valuable organic compounds that are having medicinal applications and have a beneficial effect on kidney stone problem.


2019 ◽  
Vol 5 (2) ◽  
pp. 138-145
Author(s):  
Namadina Muhammad Murtala ◽  
Nuhu Aliyu ◽  
Aliyu Bala Sidi ◽  
Hafizu Muhammad Sulaiman ◽  
Ahmad Abdulkadir Tahir ◽  
...  

Nymphaea lotus belongs to the family Nymphaeaceae and traditionally used in the treatment of aphrodisiac, anodyne, astringent, cardiotonic, sedative, demulcent, analgesic and as anti-inflammatory agent. The objective of the study was to evaluate the phytochemical, physicochemical analysis and safety margin of N. lotus rhizome with the hope of assisting in its standardization for quality, purity and safety. The powdered sample of the rhizome was extracted with aqueous and methanol and evaluated for physicochemical parameters of the plant. The extracts were subjected to qualitative and quantitative phytochemical analysis and acute toxicity study. The physicochemical parameters evaluated include: moisture content (7.4%), total ash (10.3%), water soluble (7.1%), acid insoluble (2.8%), ethanol extractive value (16.7%), and water extractive value (22.0%). The quantitative phytochemical analysis revealed that alkaloids (166.0 mg/g) was the highest phytochemical detected in the rhizome while the lowest was saponins (22.0 mg/g). LD50 of both extracts was above 5000 mg/kg and did not cause mortality in all the tested rats. The results of this finding may be useful in laying down standards and for the compilation of a suitable pharmacopoeia parameters on N. lotus. Asian J. Med. Biol. Res. June 2019, 5(2): 138-145


2020 ◽  
pp. 290-304
Author(s):  
Amrin Naimoddin Mirza ◽  
Satish S Patil

The aim of study was to investigate the seasonal changes of physicochemical parameters in the soil of selected 15 spots of the Gautala reserve forest. The soil is consist of the most significant natural factors, which is created by weathering of the rocks existing in the environment. The present survey was made to study consists the physicochemical parameters in the soil of the Gautala Reserve Forest during the year 2017-18 at three seasonal intervals i.e. rainy, winter and summer seasons. The soil parameters examined were soil pH, soil texture, moisture content, bulk density, electrical conductivity (EC),organic carbon (OC), available Nitrogen (N), available Phosphorus (P), and available Potassium (K). The Gautala forest soil physical property was analyzed as sandy loam and silty loam. The pH and Organic carbon levels were low during summer and high in monsoon. The total amount of available N, P, K, electrical conductivity, water holding capacity and moisture content were maximum in monsoon and minimum in summer season. The physicochemical properties are dependent variables that play an important role in order to understand plant diversity.


Author(s):  
N. Michael Antony ◽  
Jennifer Fernandes ◽  
Jane Mathew

Aims: To carry out extraction, preliminary phytochemical analysis and invivo analgesic screening of extract of the stem of Passiflora foetida L. Methodology: Passiflora foetida L; Family: Passifloraceae, is an exotic fast-growing perennial and medicinal vine occurring in Germany, France and other European countries and USA and grown in different parts of India. Dried stems of Passiflora foetida L was coarsely powdered and maceration was done using Soxhlet apparatus. The ethanolic extract of stems of Passiflora foetida L was subjected to preliminary phytochemical tests. Then subjected to in vivo analgesic activity. Results: Phytochemical investigation of the stem of Passiflora foetida L preliminary test showed the presence of carbohydrates, glycosides, flavonoids and steroids. Acute toxicity study of ethanolic extract of stems of Passiflora foetida L was carried out and extracts were found to be safe up to 2000 mg/kg body weight. Pharmacological activities of stems of Passiflora foetida L was carried out from ethanolic extract. Conclusion: Phytochemical investigation of ethanolic extract of stems were carried out and Analgesic activity by tail flick method in rats and acetic acid induced writhing method in mice, showed statistically significant activity (P=.05) when compared to control. The ethanolic stem extract of Passiflora foetida L proved to have significant pain relieving action in a dose dependent manner.


Sign in / Sign up

Export Citation Format

Share Document