scholarly journals Penentuan Aktivitas Antioksidan dan Kandungan Flavonoid Total Ekstrak Daun Papasan (Coccinia grandis L.) Berdasarkan Perbedaan Pelarut Polar

KOVALEN ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 30-41
Author(s):  
Meliani Sari ◽  
Rani Nareza Ulfa ◽  
Mauritz Pandapotan Marpaung ◽  
Purnama
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Water Extract ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Polar Solvents ◽  
Coccinia Grandis ◽  
Minor Metabolite ◽  
Ic50 Values

Papasan leaves have several properties, including antihyperglycemic, diabetes, antipyretic and antioxidant. Minor metabolite compounds that have the potential as antioxidants from this plant are flavonoids, alkaloids, saponins, and tannins. This study's purpose to determine the antioxidant activity and the flavonoid total contents of Papasan (Coccinia grandis L.) leaf extract based on the differences in polar solvents. The polar solvents used were ethanol, methanol, and water. Extraction used by maceration and dekokta. The antioxidant activity and the flavonoid total contents were established by UV-Vis spectrophotometry according to the decrease in absorbance at a maximum wavelength of 515 nm by the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Determination of antioxidant activity and the flavonoid total contents were excluded on quercetin comparisons. The values of  IC50 and the flavonoid total contents in the ethanol extract respectively were 287.92 ppm and 49.825 mg QE/g while the IC50 values and total flavonoid levels in the extract of methanol were 73.29 ppm and 50.415 mg QE/g. In the extract of the water, the value of IC50 was 39.80 ppm and the total flavonoid contents were 50.415 mg QE/g. The conclusions of these studies were that the most antioxidant activity was found in water extract, while water and methanol extract had the same and highest levels of total flavonoids.

scholarly journals DETERMINATION OF THE ANTIOXIDANT ACTIVITY OF PRASMAN LEAF EXTRACTS (AYAPANA TRIPLINERVIS [VAHL]) AND THE TOTAL FLAVONOID AND PHENOL CONTENTS OF THE MOST ACTIVE EXTRACTS

2020 ◽  
pp. 107-111
Author(s):  
YUNITRIANTI ◽  
BERNA ELYA ◽  
ARIKADIA NOVIANI
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Total Flavonoid ◽  
Dpph Assay ◽  
Frap Assay ◽  
Methanol Extracts ◽  
Uv Visible ◽  
Antioxidant Effects

Objective: Prasman (Ayapana triplinervis [Vahl]) is a plant that can eliminate free radicals through its antioxidant effects. However, little research hasbeen conducted to explore the antioxidant activity of the plant.Methods: A. triplinervis (Vahl) leaves, which were determined by the Indonesian Institute of Sciences (LIPI) Bogor, were used in this study. Meanwhile,extraction was performed using n-hexane, ethyl acetate, and methanol as the solvents. Based on the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay usinga UV–visible spectrophotometer and based on ferric reducing antioxidant power (FRAP) assay using a microplate reader.Results: Based on the DPPH assay using a UV–visible spectrophotometer, n-hexane, ethyl acetate, and methanol extracts with a final concentrationof 25 μg/mL inhibited DPPH radical production by 38.91, 51.03, and 54.06%, respectively. Using the percent inhibition, the IC50 for the ethyl acetateand methanol extracts were 28.71 and 23.472 μg/mL, respectively. Based on FRAP assay using a microplate reader, the n-hexane, ethyl acetate, andmethanol extracts had ferrous equivalent antioxidant capacity values of 460, 828.99, and 940.22 μmol/g, respectively. The methanol extract had thegreatest antioxidant activity. The ethyl acetate and methanol extracts at the initial concentrations contained total phenol levels of 12.06 and 42.11 mgGAE/g extract, respectively, as well as total flavonoid levels of 3.24 and 3.41 mg QE/g extract, respectively.Conclusion: Based on the determination of phenol and flavonoid levels, the methanol extract had the greatest antioxidant effects.

scholarly journals Phytochemical Composition, Antioxidant Activity, and Neuroprotective Effect ofTerminalia chebulaRetzius Extracts

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Chia Lin Chang ◽  
Che San Lin
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Neuroprotective Effect ◽  
Water Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Cupric Sulfate ◽  
Neuroprotective Effects

The objectives of this study were to determine phytochemical compositions, chemiluminescence antioxidant activities, and neuroprotective effects on PC12 cells for water, methanol, and 95% ethanol extracts of the air-dried fruit ofTerminalia chebulaRetzius. The water extract afforded the greatest yield, and total phenolic and tannin content. The methanol extract yielded the greatest total triterpenoid content. Based on four chemiluminescence antioxidant assays, the three extracts showed various degrees of antioxidant activity. The methanol extract showed good antioxidant activity based on the horseradish peroxidase-luminol-hydrogen peroxide (H2O2) assay. The water extract appeared to have good antioxidant activities in cupric sulfate-Phen-Vc-H2O2and luminol-H2O2assays. Pyrogallol-luminol assay showed the 95% ethanol extract to have good antioxidant activity. The methanol and water extracts presented neuroprotective activities on H2O2-induced PC12 cell death at 0.5–5.0 μg/mL. Further investigations are necessary to verify these activitiesin vivo.

scholarly journals Determination of Total Phenolic, Total Flavonoid and Antioxidant Activity of Karamunting Leaves (Rhodomyrtus tomentosa (Aiton) Hassk) Extract and Fraction

2021 ◽  
Vol 9 (2) ◽  
pp. 72-78
Author(s):  
Rahma Dona
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Total Flavonoid ◽  
Hexane Fraction ◽  
Total Phenolic ◽  
Water Fraction ◽  
Ic50 Value

Karamunting (Rhodomyrtus tomentosa (Aiton) Hassk) is a family of Myrtaceae which has been used by the community as traditional medicine. The purpose of this research is determine total phenolic and total flavonoid level and test antioxidant activity of Karamunting Leaves. Determination of total phenolic using gallic acid standard by the Folin Ciocalteu method. The results obtained total phenolic in water fraction of 99 mgGAE/g, ethanol extract 94,1 mgGAE/g, ethyl acetate fraction 83,3 mgGAE/g and n-hexane fraction 41,4 mgGAE/g. Determination of total flavonoid using standard quersetin with AlCL3 complex formation method, the results obtained total flavonoid in water fraction of 156,8 mgQE/g, ethanol extract 192,6 mgQE/g, ethyl acetate fraction 89,4 mgQE/g and n-hexane fraction 31,3 mgQE/g. Determination of antioxidant activity was determined by DPPH radical capture test (1,1-diphenyl-2-picrylhydrazyl), the results obtained in water fraction with IC50 value 15,02 µg/mL, ethanol extract 14,06 µg/mL, ethyl acetate fraction 14,48 µg/mL with very strong activity of antioxidant, whereas in the n-hexane fraction was categorized as weak antioxidant activity with an IC50 value of 264,02 µg/mL.

scholarly journals Spectrophotometric Determination of Total Flavonoid Content in Biancaea Sappan (Caesalpinia sappan L.) Leaves

2021 ◽  
Vol 8 (3) ◽  
pp. 1-4
Author(s):  
Nurlinda Nurlinda ◽  
Virsa Handayani ◽  
Faradiba Abdul Rasyid
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Total Flavonoid ◽  
Total Flavonoids ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Caesalpinia Sappan ◽  
Antimicrobial Treatments

AbstractBiancaea sappan  (BS) is traditionally used to treat anticonvulsants, anti-inflammatory, antiproliferative, anticoagulant, antiviral, immunostimulant, antioxidant, and antimicrobial treatments. Flavonoids are found in Secang; flavonoids are secondary metabolites that have antioxidant activity. This study aims to identify the flavonoids using TLC and determination of flavonoids content in BS leaves. Initially, The methanol extract of BS was obtained by maceration with ethanol. The qualitative analysis of flavonoid was using TLC and visualization by sprayed with AlCl3. The determination of total flavonoid content is conducted based on the AlCl3 method with total flavonoids expressed in QE (Quercetin equivalent) at the maximum wavelength of 431 nm. The research results showed that BS leaves contain flavonoids and the total flavonoid content of BS leaf extract is 1.0318 mg QE / g extract.Keywords: Caesalpinia sappan; Flavonoid content, Spectrophotometric

scholarly journals Comparison of Antioxidant Activity of Ethanolic, Methanolic, n-Hexan, and Aqueous Extract of Parkia speciosa Peel based on Half -Maximal Inhibitory Concentration Through Free Radical Inhibition

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Fafa Nurdyansyah ◽  
Dyah Ayu Widyastuti
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Water Extract ◽  
Bark Extract ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Rotary Vacuum ◽  
Potential Content

The objectives of this study was to determine the half maximum inhibitory concentration (IC50) from four types of Parkia speciosa peel extracts (ethanol, methanol, n-hexane, and aqueous)  through DPPH free radical inhibition. First Parkia’s peel extract made by drying the Parkia’s peel that has been sorted, then crushed and mashed with a blender. Parkia’s powder then macerated for 3 replication using each type of solvent and then solvent evaporation was carried out using a rotary vacuum evaporator. The evaporated extract produced then tested for antioxidant activity using the IC50 method and phytochemical screening was performed to analyze the potential content of functional compounds. The results showed that all types of solvents dissolve alkaloid compounds (except water extract), flavonoids, saponins, tannins, and phenols. IC50 values produced from the four types of petai bark extract using methanol, ethanol, water, and n-hexane solvents sequentially were 76.92; 111; 136; and 201 ppm. Methanol extract had the lowest IC50 value of 76.92 ppm which resulted that the methanol extract of petai skin had a strong (active) antioxidant strength compared to others.Keywords: Antioxidant, Exctract, Parkia’s peel, IC50

scholarly journals Total Phenol and Antioxidant Activity of Ethanol Extract and Water Extract from Claw Uncariaa gambir Roxb

2021 ◽  
Vol 9 (3) ◽  
pp. 131
Author(s):  
Lucky Hartanti ◽  
Asri Mulya Ashari ◽  
Warsidah Warsidah
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Water Extract ◽  
Ethanol Extract ◽  
Total Phenol ◽  
Total Phenolic ◽  
Market Demand ◽  
University Campus ◽  
Industrial Plants

Uncaria gambier Roxb is a plant from the Rubiaceae family, belongs to the Bajakah group and has been used empirically in the treatment of various types of diseases in the community. Besides being one of the industrial plants and export commodities produced in Sumatra and Kalimantan, gambier leaves is also used frequently in Indonesia, both for the pharmaceutical and cosmetic industries. As a result, market demand both nationally and internationally have increased. The aim of this research are determination of antioxidant activity of ethanol extract and aqueous extract of gambir claw using 1,1-diphenil-2-picrylhydrazyl (DPPH) and determination of total phenol using Calcetau folin reagent. Gambier plant samples were taken in the forest area around the Tanjungpura University campus, the claws were separated and dried at room temperature, then powdered and extracted by maceration using 70% ethanol pa and aquadest, then the extract was concentrated until it was ready to be tested for antioxidant activity and determination of total phenol. The total phenolic ethanol extract and aqueous extract of gambir claw were 224.66 mg GAE/g extract (GEA : Gallic Acid Eqivalent) and 299.08 mg GAE/g extract, respectively, while the antioxidant potential (IC50) was 39.566 μg/mL and 65.140 μg/mL, and the IC50 for comparison of Vitamin C was 7.02 μg/mL.

scholarly journals Determination of Total Flavonoid Contents and Antioxidant Activity of Leucaena Leucocephala Leaves's Extract

2020 ◽  
Vol 9 (2) ◽  
pp. 70-77
Author(s):  
Taupik Hidayat ◽  
Baharuddin Hamzah ◽  
Minarni R. Jura
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Leucaena Leucocephala ◽  
Positive Control ◽  
Total Flavonoid ◽  
Antioxidant Vitamin ◽  
Antioxidant Power ◽  
Sample Extract ◽  
Ic50 Values

The study of the determination of total flavonoid contents and antioxidant activity of extract of Leucaena leucocephala leaves had been carried out using UV-Vis spectrophotometry method. This study aimed to determine the total flavonoid contents and the antioxidant power of Leucaena leucocephala extract. Extraction of chemical content from Leucaena leucocephala was carried out by the maceration method using absolute ethanol. The total flavonoid contents from the sample extract were taken up for absorption at wavelength 510 nm and 700 nm. The variation of the concentration of Leucaena leucocephala leaves' extract was respectively 20, 40, 60, and 80 ppm. The results of the study showed that the total flavonoid contents of extracts of Leucaena leucocephala leaves was 2.242 g/100 grams. The antioxidant activity of Leucaena leucocephala leaves extract was carried out using DPPH (1,1-diphenyl-2-picrylhydrazine) and the measurement of absorption was at a wavelength of 517 nm, and by varying the sample concentration. Positive control used was vitamin C solution with the same variation of extract's concentration. The results showed that the IC50 values of Leucaena leucocephala leaves extract and vitamin C were 175.388 and 28.907 ppm. Based on the IC50 data it can be stated that the antioxidant vitamin C was stronger than the Leucaena leucocephala leaves extract which was classified as a weak type of natural antioxidant.

scholarly journals PHYTOCHEMICAL SCREENING, TOTAL FLAVONOID, ANTIOXIDANT ACTIVITY, AND TOXICITY OF ETHANOL EXTRACT Cleome gynandra L. HERB

2018 ◽  
Vol 3 (2) ◽  
pp. 41
Author(s):  
Agustinus Widodo ◽  
Ritha Pratiwi
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Phytochemical Screening ◽  
Food Ingredients ◽  
The People ◽  
Cleome Gynandra ◽  
Dpph Method ◽  
Central Sulawesi

<em>Cleome gynandra L. is one of the plants that the people of Palu, Central Sulawesi use as food ingredients. This plant is empirically used in traditional medicine. This study aims to determine phytochemical constituents, total flavonoid, antioxidant activity, and toxicity of 96% ethanol extract of C. gynandra herb. C. gynandra herb extract was obtained by maceration. Phytochemical screening of the ethanol extract was carried out qualitatively according to the standard methods. Determination of total flavonoid using AlCl<sub>3</sub> then determined by Spectrophotometric UV-Vis. Antioxidant activity using the DPPH method and determined IC<sub>50</sub> value. Toxicity test was assessed using shrimp lethality as an indicator of toxicity. Phytochemical screening showed 96% ethanol extract containing alkaloid compounds, flavonoids, saponins, steroids, and tannins. Total flavonoid of the 96% ethanol extract was 4,778 ± 0,522 mg QE/g extract. Antioxidant activity (IC<sub>50</sub>) of the ethanol 96% extract was 189,455 µg/ml. Lethal concentration 50% of the 96% ethanol extract was 472,648 mg/L (toxic). The results of this study indicate that 96% ethanol extract of C. gynandra herb has antioxidant activity and has the potential to be further tested as an anticancer activity.</em>

Validasi dari Spektrofotometri UV-Vis dan Kandungan Total Flavonoid Ekstrak Etanol dari Akar Alang-Alang (Imperata cylindrica) dan Daun Pegagan (Centella asiatica) Anita Puspa Widiyana

2021 ◽  
Vol 3 (2) ◽  
pp. 126-136
Author(s):  
Anita Puspa Widiyana ◽  
Keyword(s):  
Chemical Constituents ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Imperata Cylindrica ◽  
Total Flavonoid ◽  
Analysis Method ◽  
Flavonoid Content ◽  
Validation Testing

Validation as a quality control for the content of chemical compounds from natural ingredients. One of the chemical constituents is the flavonoids which are found in the Imperatacylindrica roots and Centella asiatica leaves. This study aims to ensure the analysis method meets the requirements and determines the levels of flavonoids. The research stages included extraction, validation and determination of total flavonoid. Extraction was carried out by immersing dry simplicia in 96% ethanol solvent for 3x24 hours. The solvent is evaporated using a rotary evaporator until a thick extract is formed. Validation testing includes linearity, accuracy, precision, LOD and LOQ. Determinationof the total flavonoid was carried out by measuring the absorption at a maximum wavelength of 428.2 nm. The validation results includethe correlation coefficient (R) of 0.998, precision % RSD <2 %, %accuracy 99,53-97,98%, LOD 3.02ppm and LOQ 9.15 ppm. The total flavonoid of the ethanol extract of Imperata cylindrica roots was 36.39 ± 0.08 mg/g QE and Centella asiatica leaves was 102.10 ± 0.08 mg/g QE. The conclusion is that the method used met the validation requirements and the total flavonoid content of the ethanol extract of Centella asiatica leaves was higher than Imperata cylindrica roots

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL BULBUS BAWANG DAYAK (Eleutherine americana Merr) DENGAN METODE DPPH (1,1-Diphenyl-2-Picrylhydrazyl)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 451
Author(s):  
Rizkah V. Mokoginta ◽  
Herny E. I. Simbala ◽  
Karlah L.R Mansauda
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Onion Plant ◽  
Dpph Method ◽  
Ic50 Values ◽  
Experimental Laboratory ◽  
Ethanol Extracts ◽  
Eleutherine Americana

ABSTRACTDayak onion plant (Eleutherine americana Merr.) or commonly called forest onion is a typical plant of Central Kalimantan that has been used by local peoples for cure a various types of diseases. This study aims to determine the activity of antioxidant compounds from Dayak bulb ethanol extracts from Kotamobagu City. This research method is an experimental laboratory. Initially the Dayak bulb sample was extracted maceration with 96% ethanol solvent then evaporated until a thick extract was obtained. As a parameter, the DPPH method (1,1-diphenyl-2-picrylhydrazyl) was used to test the antioxidant activity of the samples at concentrations of 50, 60, 70, 80 and 90 mg / L and as a comparison standard used Vitamin C with concentrations of 2, 4, 6, 8 and 10 mg / L. The results obtained showed antioxidant activity in Dayak bulb ethanol extracts with IC50 values of 41.46 mg / L and on vitamin C IC50 values of 1.04 mg / L. Based on these results it can be concluded that the onion bulb ethanol extract has very strong antioxidant activity. Keywords: Dayak Onion bulbs, Activity, Antioxidant, DPPH  ABSTRAK Tanaman bawang Dayak (Eleutherine americana Merr.) atau biasa disebut bawang hutan merupakan tanaman khas Kalimantan Tengah yang sudah dipergunakan masyarakat lokal sebagai obat berbagai jenis penyakit. Penelitian ini bertujuan untuk mengetahui aktivitas senyawa antioksidan dari ekstrak etanol bulbus bawang Dayak yang berasal dari Kota Kotamobagu. Metode penelitian ini adalah eksperimental laboratorium. Awalnya sampel bulbus bawang Dayak diekstraksi maserasi dengan pelarut etanol 96% kemudian dievaporasi hingga didapatkan ekstrak kental. Sebagai parameter, digunakan metode DPPH (1,1-diphenyl-2-picrylhydrazyl) untuk pengujian aktivitas antioksidan sampel pada konsentrasi 50, 60, 70, 80 dan 90 mg/L dan sebagai standar pembanding digunakan Vitamin C dengan konsentrasi 2, 4, 6, 8 dan 10 mg/L. Hasil penelitian yang didapat menunjukkan aktivitas antioksidan pada ekstrak etanol bulbus bawang Dayak dengan nilai IC50 sebesar 41,46 mg/L dan pada vitamin C nilai IC50 sebesar 1,04 mg/L. Berdasarkan hasil tersebut dapat disimpulkan bahwa ekstrak etanol bulbus bawang Dayak memiliki aktivitas antioksidan yang sangat kuat. Kata kunci: Bulbus Bawang Dayak, Aktivitas, Antioksidan, DPPH

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