scholarly journals Role of Harderian gland in immune response of chickens with maternal immunity to vaccine against infectious bronchitis virus

2012 ◽  
Vol 66 (3-4) ◽  
pp. 211-218
Author(s):  
Ljiljana Spalevic ◽  
Vojin Ivetic ◽  
Dobrila Jakic-Dimic ◽  
Danka Maslic-Strizak ◽  
Aleksandar Potkonjak ◽  
...  
Keyword(s):  
Immune Response ◽  
Negative Impact ◽  
Harderian Gland ◽  
Vaccine Virus ◽  
Elisa Test ◽  
Lymphoid Organ ◽  
Control Group ◽  
Broiler Chicks ◽  
Infectious Bronchitis

In this study we investigated the role of the Harderian gland in the immune response following vaccination against infectious bronchitis (IB). The experiment was carried out on 100 broiler chicks which were divided into two experimental groups. Experimental group O was vaccinated on the first day of age against infectious bronchitis with vaccine Bronhivet I batches that contain a live attenuated strain H120 and the control group did not get immunized. The experiment lasted 21 days. Blood sera were examined on days 1, 7, 14 and 21 for the presence of specific antibodies against infectious bronchitis (ELISA test). Histological changes were observed in the structure of the Harderian gland in relation to the applied vaccine. This research demonstrated the role of the Harderian gland as a secondary lymphoid organ in broiler vaccination against infectious bronchitis. Maternal antibodies present in the IB had no negative impact on the vaccination of chickens on the first day of age. The vaccine virus partially damaged the Harderian gland.

scholarly journals EFFECT OF VITAMIN E AND COD LIVER OIL SUPPLEMENT WITH BIVALENT OIL BASED VACCINE OF NEWCASTLE DISEASE AND INFECTIOUS BRONCHITIS DISEASE ON IMMUNE RESPONSE OF THE BROILERS.

2016 ◽  
Vol 47 (3) ◽  
Author(s):  
ABDULWAHID & et al.
Keyword(s):  
Immune Response ◽  
Vitamin E ◽  
Newcastle Disease ◽  
Challenge Test ◽  
Elisa Test ◽  
The Other ◽  
Control Group ◽  
Broiler Chicks ◽  
Cod Liver Oil ◽  
Infectious Bronchitis

This study was aimed to enhance the immune response of the broilers by vitamin E and Cod liver oil supplement with locally inactivated bivalent vaccine of Newcastle disease and Infectious bronchitis viruses. One hundred and twenty broiler chicks Ross 308 were divided to four groups at 30 chicks of each group. The 2nd group was vaccinated with 0.5ml/chick of locally inactivated ND-IB vaccine at 5 days old. The 3rd group was vaccinated with 0.5ml locally ND-IB vaccine and 0.1 ml/chick of VE. The 4th group was vaccinated with 0.5ml of locally ND-IB vaccine and 0.1ml/chick of Cod liver oil, while the first group was left without vaccination as a control group. All groups were vaccinated with attenuated ND-IB at 3 days old and attenuated IBD at 14 days old. The results of ELISA test revealed that the 3rd and 4th groups showed significantly increased (P≤0.05) in Ab titers against ND virus at aged 35 days, also, the 4th group showed significantly (P≤0.05) increased in Ab titers against IB virus at day 35 of age as compared with the other groups. The 4th group showed a significant (P≤0.05) increased in bursal index as compared with the other groups. The results of challenge test revealed that vaccinated groups resist the velogenic ND and IB viruses with relatively high productive range particularly the 4th group as compared with other groups. In conclusion, the VE and Cod liver oil supplement with local bivalent oil based vaccine of ND and IBV may enhance the immune response of the broilers particularly the 4th group which vaccinated with 0.5 ml of local bivalent oil based vaccine of ND-IB vaccine and 0.1 ml Cod liver oil.

scholarly journals Morphofunctional condition of the harderian gland of chickens during the post-vaccine immunity to infectious bronchitis

2020 ◽  
Vol 7 (1) ◽  
pp. 32-39
Author(s):  
S. Guralska ◽  
T. Kot ◽  
O. Pinskyi ◽  
V. Sokolyuk ◽  
T. Budnik
Keyword(s):  
Harderian Gland ◽  
Morphometric Study ◽  
Control Group ◽  
Infectious Bronchitis ◽  
Study Results ◽  
Vaccine Prophylaxis ◽  
Immunohistochemical Studies ◽  
Structure Patterns ◽  
Experimental Group

Aim. To fi nd out the contents and placement of CD4+, CD8+, CD45RA+, CD20+-lymphocyte subpopulations and morphocytochemical architecture of the harderian gland in clinically healthy chickens and for vaccination against infectious bronchitis. Methods. Anatomical, histological, immunohistochemical, optical, morphometric and variational-statistical. Results. The obtained morphometric study results showed that the area of secretory lobules and lymphoid formations was signifi cantly larger in the harderian gland of vaccinated 40-, 90- and 110-day-old chickens. Moreover, the organ size correlates signifi cantly with the development of lymphoid formations, since the area of the secretory lobules in the gland of the experimental group chickens does not differ from that of the control group. Immunohistochemical studies have shown that the number of lymphocytes with CD20+ markers was characterized by a reliable (P < 0.001) increase in B-lymphocytes in the harderian gland of the vaccinated chickens of all ages. Lymphocytes with CD8+ markers were not detected in the gland of vaccinated 8- and 20-day-old chickens. According to cytomorphometric studies, their number in vaccinated 40-, 90- and 110-day-old chickens was signifi cantly (P < 0.001) higher compared to intact birds – 2.11, 2.38 and 2.96 times, respectively. Conclusions. The data on the composition of lymphocytes with CD4+, CD8+, CD45RA+, CD20+ markers in the harderian gland of chickens of different age, on their changes in case of immunization against infectious bronchitis were obtained, and the role of certain clusters at different stages of the immunity formation was determined. The information on the structure, patterns of growth and development of the harderian gland of chickens, which demonstrates the incompleteness of its morphogenesis at the early stages of the postnatal ontogenesis period that should be taken into account during the compilation of vaccine prophylaxis programs, has been supplemented.

Effects of feeding Isomaltooligosaccharides on the growth performance, carcass traits and immune response of broiler chickens

2014 ◽  
Vol 3 (1) ◽  
pp. 150-157
Author(s):  
Khalid M. Gaafar
Keyword(s):  
Immune Response ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Carcass Traits ◽  
Dietary Supplementation ◽  
Control Group ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Flu Vaccine ◽  
Lipids Profile

The research was conducted to study the effect of feeding broiler chickens on diets containing isomaltooligosaccharides on the growth performance, carcass traits and immune response. 90-one day old broiler chicks were used according to completely randomized two treatment groups and one control, 30 birds each. Birds fed ad-libitum on basal starter and grower-finisher diets for 35 day. Diets of treatment`s groups contained 0.5 g/Kg and 1 g/Kg of Isomaltooligosaccharides, while the control group fed on the basal diets without Isomaltooligosaccharides supplementation. Dietary supplementation of broiler chickens with Isomaltooligosaccharides improved body weight, feed conversion, carcass traits, two lymphoid organs weight and log antibody titer against avian flu vaccine. Most of the highest values were for birds fed low levels of Isomaltooligosaccharides. Feed intake decreases as Isomaltooligosaccharides level increases. Dietary supplementation with Isomaltooligosaccharides did not affect the lipids profile (triglycerides, total cholesterol, LDL and HDL), however the blood VLDL levels decreased with increased levels of Malondialdehyde and Glutathione reductase. Collectively, Dietary supplementation of broiler chickens with 0.5 g/Kg diet of Isomaltooligosaccharides improved growth performance, carcass traits and immune status.

scholarly journals The Role of Serotonin in MDMA Self-administration in Rats

2021 ◽  
Author(s):  
◽  
Sarah Bradbury
Keyword(s):  
Negative Impact ◽  
Primary Objective ◽  
Repeated Exposure ◽  
Lesion Group ◽  
Control Group ◽  
Self Administration ◽  
Initial Exposure ◽  
Abstinence Period

<p>Rationale: The profile of acquisition for MDMA self-administration differs from that of amphetamine and cocaine self-administration in that fewer rats meet an acquisition criterion and the latency to acquisition is longer. These characteristics of MDMA self-administration may be because it preferentially stimulates serotonin (5HT) release whereas self-administration has generally been attributed to enhanced dopamine (DA) neurotransmission. Because 5HTergic agonists are not self-administered and increased synaptic 5HT decreased self-administration of other drugs, MDMA self-administration may be initially inhibited by the pronounced 5HT response. Accordingly, the acquisition of MDMA self-administration might proceed as a result of deficits in 5HT neurotransmission and a corresponding disinhibition of DA neurotransmission.  Objective: The primary objective was to determine the role of 5HT in the acquisition and maintenance of MDMA self-administration.  Methods: MDMA-induced increases of extracellular 5HT and DA and their primary metabolites were measured in the DA terminal regions of the nucleus accumbens (NAc) using in vivo microdialysis, prior to the commencement of MDMA self-administration. The relationship between MDMA-induced increases of neurotransmitter levels and the acquisition of MDMA self-administration was assessed. A subsequent study depleted brain 5HT by administering the neurotoxin, 5,7 – DHT, or vehicle into the lateral ventricle of the left hemisphere, prior to the commencement of MDMA self-administration. The proportion of subjects that acquired MDMA self-administration and the latency to acquire MDMA self-administration was compared for the two groups. In order to determine effects of MDMA self-administration on 5HT and DA responses, behaviours that reflect 5HT and/or DA neurotransmission were measured 5 or 14 days after self-administration of 165 mg/kg MDMA, or 14 days after vehicle self-administration. These time periods were chosen because they reflect a period of 5HT deficits (5 days) and recovery (14 days). Finally, the effect of abstinence on MDMA self-administration was measured.  Results: The MDMA-induced increase of extracellular 5HT was significantly lower for the group that subsequently acquired MDMA self-administration but the MDMA-induced increase in DA was not different from the group that failed to acquire self-administration. 5, 7-DHT administration significantly decreased tissue levels of 5HT, but not DA. MDMA self-administration was facilitated by the lesion; 100% of the lesion group acquired MDMA self-administration, whereas only 50% of the control group acquired self-administration. Five days following the last MDMA self-administration session, DAergic behaviours were enhanced and 5HTergic behaviours were reduced relative to the control group. These differences in 5HTergic mediated behaviours were not apparent 14 days after self-administration but the DAergic behaviours remained elevated. The pattern of self-administration did not differ as a function of the length of the abstinence period.  Conclusions: The variability in acquisition of MDMA self-administration was related to the magnitude of the 5HT response evoked by initial exposure to MDMA. These findings suggested that predisposing differences in the 5HT response might explain differences in the variability in acquisition of MDMA self-administration. The negative impact of 5HT on the acquisition of MDMA self-administration was clearly demonstrated following a 5, 7-DHT lesion. Thus, 5HT limits the development of MDMA self-administration. With repeated exposure to self-administered MDMA, behavioural responses indicative of 5HT activation were reduced whereas behavioural indices of DA activation were increased. The maintenance of MDMA self-administration was comparable regardless of whether there was a forced abstinence period or not. These data are consistent with the hypotheses that 5HT is inhibitory to the acquisition, but not the maintenance, of MDMA self-administration. Rather, the maintenance of self-administration might reflect sensitised DA responses that became apparent following repeated exposure.</p>

scholarly journals Route of infectious bronchitis virus vaccination determines the type and magnitude of immune responses in table egg laying hens

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Mohammed Al-Rasheed ◽  
Christopher Ball ◽  
Kannan Ganapathy
Keyword(s):  
Immune Responses ◽  
Infectious Bronchitis Virus ◽  
Laying Hens ◽  
Early Time ◽  
Harderian Gland ◽  
Vaccine Virus ◽  
Egg Laying ◽  
Immune Gene ◽  
Mrna Levels ◽  
Infectious Bronchitis

AbstractChicken immune responses to infectious bronchitis virus (IBV) vaccination can depend on route of administration, vaccine strain and bird age. Typically for layer chickens, IBV vaccinations are administered by spray in the hatchery at day-old and boosted at intervals with live vaccines via drinking water (DW). Knowledge of live attenuated IBV vaccine virus kinetics and the immune response in egg-laying hens is exceptionally limited. Here, we demonstrated dissemination of vaccine viruses and differences in hen innate, mucosal, cellular and humoral immune responses following vaccination with Massachusetts or 793B strains, administered by DW or oculonasal (ON) routes. Detection of IBV in the Mass-vaccinated groups was greater during early time-points, however, 793B was detected more frequently at later timepoints. Viral RNA loads in the Harderian gland and turbinate tissues were significantly higher for ON-Mass compared to all other vaccinated groups. Lachrymal fluid IgY levels were significantly greater than the control at 14 days post-vaccination (dpv) for both vaccine serotypes, and IgA mRNA levels were significantly greater in ON-vaccinated groups compared to DW-vaccinated groups, demonstrating robust mucosal immune responses. Cell mediated immune gene transcripts (CD8-α and CD8-β) were up-regulated in turbinate and trachea tissues. For both vaccines, dissemination and vaccine virus clearance was slower when given by DW compared to the ON route. For ON administration, both vaccines induced comparable levels of mucosal immunity. The Mass vaccine induced cellular immunity to similar levels regardless of vaccination method. When given either by ON or DW, 793B vaccination induced significantly higher levels of humoral immunity.

scholarly journals The Specific Immune Response after Vaccination against Neonatal Calf Diarrhoea Differs between Apparent Similar Vaccines in a Case Study

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1238
Author(s):  
Román Gonzalez ◽  
Laura Elvira ◽  
Carlos Carbonell ◽  
Geert Vertenten ◽  
Lorenzo Fraile
Keyword(s):  
Immune Response ◽  
Negative Impact ◽  
Calf Serum ◽  
Group Versus ◽  
Study Groups ◽  
Negative Control ◽  
Specific Immune Response ◽  
Control Group ◽  
Neonatal Calf ◽  
Calf Diarrhoea

Neonatal calf diarrhoea (NCD) is a major health challenge with a negative impact on farm profitability, calf welfare and antimicrobial use. Neonatal calves are particularly sensitive to enteric infections. Thus, a key point for prevention is minimising infectious pressure and maximising specific immune responses. An amount of 120 dams not previously vaccinated against NCD were randomly allocated to one of three study groups: negative control versus two vaccinated groups (A and B). In the control group, the average level of antibodies was significantly low for both BoCV and ETEC (15.6 and 13.9% in the colostrum samples, respectively), demonstrating the importance of dam vaccination. Indeed, the level of specific immunity was significantly increased for BoCV and ETEC with dam vaccination using both one-shot vaccines versus the control group. Moreover, the statistical analysis revealed a significantly higher level of antibodies for BoCV and ETEC in colostrum samples in vaccine A versus vaccine B and the control group. In accordance, the calf serum demonstrated a significantly higher level and greater homogeneity of antibodies against BoCV and ETEC in the Vaccine A group versus other experimental groups (p < 0.05). In conclusion, this study demonstrated a different specific immune response for the pathogens depending on the vaccine used to control NCD in cows.

scholarly journals The effect of egg powder inclusion in the pre-starter diet on the immune response of male broiler chickens

2013 ◽  
Vol 56 (1) ◽  
pp. 527-535 ◽  
Author(s):  
L. Esmailzadeh ◽  
M. Shivazad ◽  
A. A. Sadeghi ◽  
M. Karimi-Torshizi
Keyword(s):  
Immune Response ◽  
Disease Virus ◽  
Antibody Titre ◽  
Broiler Chickens ◽  
Relative Weight ◽  
Control Group ◽  
Infectious Bronchitis ◽  
Significant Difference ◽  
Bursal Disease ◽  
Egg Powder

Abstract. A completely randomised design was performed to evaluate the effect of inclusion of egg powder in the pre-started diet (1–7 day age) on the immune response of male broiler chickens. Experimental pre-starter diets formulated to have 0, 20, 40 or 60 g/kg egg powder. The relative weight of lymphoid organs (spleen and bursa) was reduced, in days 7 and 42 of age, by graded inclusion of egg powder in the pre-starter period (P≤0.05). The highest antibody titre against Newcastle disease virus (NDV) and Infectious bronchitis virus (IBV) in days 7 and 42 of age was for chickens fed the diet containing 60 g/kg egg powder in the pre-starter diet (P≤0.05). At day 7 of age, birds fed the diet containing egg powder had a higher antibody titre against Infectious bursal disease virus (IB) compared to the control group (P≤0.05), but at day 42 of age, there was no significant difference between the treatments (P>0.05). There was no difference (P>0.05) among the treatments for the titre against sheep red blood cells and immune response against dinitrochlorobenzene. It was concluded that the inclusion of egg powder to the pre-starter diet could significantly enhance the immune response of the male broiler chickens.

scholarly journals The impact of Levamisole on Newcastle disease antibodies titer in broilers fed with diet contaminated with Aflatoxin

2006 ◽  
Vol 30 (1) ◽  
pp. 35-42
Author(s):  
Hatem M. M. AL- Naemey
Keyword(s):  
Immune Response ◽  
Drinking Water ◽  
Newcastle Disease ◽  
Elisa Test ◽  
The Third ◽  
Suppressor Effect ◽  
The Impact ◽  
Newcastle Disease Vaccine ◽  
Protection Ratio

This study designed to know the immuno suppressor effect of aflatoxinsand the efficacy of levamisole to reduce these effects in the titer of antibodiesand protection against challenge for Newcastle disease vaccine in chickens.This trail include three groups, first group fed on ration free fromaflatoxins contamination, Second group fed on ration contaminated withaflatoxins, while the third group fed on the same ration of second group andgiven levamisole via drinking water with the dose of 10mg/kg of body weight.All groups vaccinated twice against Newcastle disease at 10 and 20 daysof age. The immunity evaluated by ELISA test at 6, 19, 31 days of age andchallenged at 32 days old.The results indicate that the second group has the lowest titer ofantibodies and protection ratio after challenge, while the first group has thehightest titer of antibodies and high protection ratio after challenge. The titer ofantibodies and the protection against challenge in the third group had higher titerthan them at second group and lower than them at first group.These results revealed the role of aflatoxins in immunosuppression forNewcastle vaccines and reduce the efficacy of vaccination process and reducethe protection ratio after challenge, also reveal the role of levamisole in reducingof these effect and elevation of immune response to Newcastle vaccine inchickens.

scholarly journals EVALUATING THE ROLE OF IL-2 AND IL-6 IN PATIENTS WITH BURNS USING ELISA TECHNIQUE

2019 ◽  
Vol 1 (2) ◽  
pp. 85-89
Keyword(s):  
Immune Response ◽  
Age Groups ◽  
Adaptive Immune Response ◽  
Control Group ◽  
Serum Samples ◽  
Adaptive Immune ◽  
Significant Difference ◽  
Elisa Technique ◽  
Male Patients

Introduction: Burns are common medical infections that examined in hospitals. Cytokines are produced by innate immune response; cytokines determine the type of adaptive immune response. This study aims to screen and evaluate the role of IL-2 and IL-6 levels in the serum of patients who have suffered from burns by ELISA technique. Methods: Seventy serum samples were collected from burned patients in Baghdad city hospitals and tested by ELISA technique to detect IL-2 and IL-6 levels. Results: Shows great differences in IL-2 level of male patients (30.16 pg/ml) compared to males control group by an average of (29.66 pg/ml). While IL-6 shows significant differences in female patients with range (63.39 pg/ml) and male (66.47 pg/ml) compared to females control group (2.48 pg/ml) and males (22.80 pg/ml). Moreover cytokines shows significant differences between the three age groups of burned patients in comparison with the control group. In conclusion the result of present study showed significant difference in level of some cytokines IL-2,IL-6 for patients with burns. Conclusion: the result of present study showed significant difference in level of some cytokines IL-2, IL-6 for patients with burns.

scholarly journals Effect of dietary stevia-based sweetener on body weight and humoral immune response of broiler chickens

2021 ◽  
Vol 14 (4) ◽  
pp. 913-917
Author(s):  
Ramón Miguel Molina-Barrios ◽  
Cielo Rubí Avilés-Trejo ◽  
María Esthela Puentes-Mercado ◽  
Jesús Raymundo Cedillo-Cobián ◽  
Juan Francisco Hernández-Chavez
Keyword(s):  
Immune Response ◽  
Body Weight ◽  
Weight Gain ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
The Body ◽  
Farm Animals ◽  
Control Group ◽  
Steviol Glycosides ◽  
Infectious Bronchitis

Background and Aim: Steviol glycosides extracted from the leaves of Stevia rebaudiana Bertoni have been of much consideration recently because of their beneficial effects on health, raising the possibilities for improving farm animals' health. Although some studies on stevia's dietary effect on body weight gain are available, few studies have been conducted to evaluate stevioside supplementation on immune response in broilers. This experiment aimed to analyze how a stevia-based sweetener can affect broiler chickens' growth performance and humoral response. Materials and Methods: In this experiment, one hundred and twenty 1-day-old Cobb-line broiler chicks fed with commercial starter/grower diets were included in three groups and supplemented with stevia-based sweetener at levels 0, 80, and 160 ppm, respectively. Chickens were weighed on day 0 and every 7 days for the next 6 weeks. Chicks were then immunized on days 10 and 24 with a Newcastle and infectious bronchitis vaccine and blood sampled on days 7, 24, and 35. Serologic assays were performed to detect specific antibody levels. Results: The body weight means and body weight gain on day 42 were found to be significantly higher in birds from the group fed with 80 ppm of stevia-based sweetener than those in the control group and slightly higher than those in the group supplemented with 160 ppm of stevia-based sweetener. Likewise, on day 35, antibodies against the Newcastle disease virus were higher in the treatment groups. Immune response to infectious bronchitis virus vaccination was not statistically different among the three groups through the experiment. Conclusion: Stevia-based sweetener at 80 ppm in commercial-based diets improved body weight gain and immune response in broiler chickens at the market age.

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