scholarly journals Possibility of using Bacillus and Trichoderma strains for decomposition of crop residues

Author(s):  
Dragana Bjelic ◽  
Jelena Marinkovic ◽  
Branislava Tintor ◽  
Jordana Ninkov ◽  
Jovica Vasin ◽  
...  

The objective of this study was to investigate the possibility of using microbial strains as residue decomposers and to determine the effect of these strains on chemical and microbial properties in the residue-amended soil. Greenhouse experiment consisted of eight Bacillus treatments, three Trichoderma treatments, and their combination, all applied to non-sterile chernozem soil amended with wheat straw. Incorporation of wheat straw improved soil chemical and microbial properties, while the extent of residue decom?position under microbial strains was intensified. Microbial treatments significantly affected the soil pH, the content of carbonate, total carbon, soil organic carbon, humus, and available phosphorus and potassium. Bacterial and fungal treatments also significantly influenced the total microbial number, ammonifiers, N2-fixers, fungi, actinomycetes, oligotrophs, copiotrophs, and cellulolytic microorganisms. The effect of microbial treatments varied depending on the applied strains and examined properties, with Bacillus strains being more promising residue decomposers compared to Trichoderma strains. The most effective microbial strains could be used as potential decomposers of crop residues.

2020 ◽  
Author(s):  
Gheorghe Stegarescu ◽  
Jordi Escuer ◽  
Karin Kauer ◽  
Endla Reintam

<p>The organic residues amendments have been widely studied for their essential role of enriching the soil with organic matter. Although the pathways of the fresh organic matter additions are very complex, so is the effect. Thus, the quality of the crop residues incorporated into the soil is a valuable attribute when deciding to switch to conservation agriculture. The different C/N ratio and biochemical composition of the crop residues will affect in various ways soil CO2, N2O and CH4 emissions and soil structural stability. The study explores the effect of different crop residues incorporated in the soil on greenhouse gas emissions and aggregate stability. The incubation experiment consisted of five treatments: control (just soil), sand (as reference), soil mixed with wheat straw, soil mixed with green fresh rye residues and soil mixed with green fresh oilseed rape residues. The residues were applied into the soil at a rate of 6 g C kg-1 of soil. The pots of all the treatments were placed for incubation for 105 days at approximately 23 oC and covered with dark plastic bags. The wetting procedure was done five times at 0-11-26-46-75 days to bring the soil to field capacity for water. The sampling for the gas emissions and aggregate stability was done before wetting and after wetting. The gas emissions were sampled using the chamber method and analysed in a Gas Chromatographer. The water-stable aggregates were analysed using the wet sieving method. The plant material was chemically analysed for total carbon and nitrogen and the biochemical composition on Fourier Transform Infrared Spectroscopy. The results revealed that the cumulative CO2 emissions in oilseed rape were 8% higher than in rye treatment. Also, it was 76% higher than in wheat straw treatment and 95 % higher than in control treatment. The highest cumulative N2O emissions were registered in rye treatment 18.79 (±0.48) mg m-2 h-1. Oilseed rape treatment had 19% lower cumulative emissions compared to rye and 98 % higher compared to control and wheat straw treatments. Both rye and oilseed rape had a low C/N ratio 12 and 10, respectively whereas wheat straw had 98 C/N ratio. From a biochemical point of view, the wheat straw was richer in stable compounds such as lignin, cellulose and hemicellulose followed by rye and oilseed rape which had a higher content of labile compounds such as sugars and easily decomposable proteins. In general mean aggregate stability increased significantly only in the wheat straw treatment being 34.69% ±1.35. </p><p>In conclusion, this study showed that crop residues with low C/N ratio have a negative effect on greenhouse emissions. But do not have a long term effect on the increase of aggregate stability. On the contrary, the wheat straw has a positive impact on greenhouse gases, and it increased aggregate stability. </p>


2016 ◽  
Vol 11 (1) ◽  
pp. 234-242
Author(s):  
Eugene Appiah-Effah ◽  
Kwabena Biritwum Nyarko ◽  
Eric Ofosu Antwi ◽  
Esi Awuah

Composting of raw faecal sludge (FS) poses many difficulties due to its high moisture content (MC), high wet bulk density and low carbon to nitrogen ratio. The aim of this study were to (1) examine the suitability of bulking materials (BMs) and (2) investigate the effect of bulking material and mixing ratio on concentration of nutrients during composting of raw FS compost. Raw FS and BMs (wood chips and maize cobs) were sampled from three peri-urban communities. The raw FS showed an initial high MC (73%) which was greater than that required to start a compost process (55–65%). The results showed that the total carbon and nitrogen contents of all the experiments decreased at the end of the composting process. Composted materials at the end of the experiment showed lower concentrations of available phosphorus and potassium in all the experiments than the initial substrate materials. Experimental trial, Maize cob (1:2) is the most preferred due to the fact that it contained more nitrogen, phosphorus, potassium and also carbon which are essential nutrients for plant growth and for improving on the soil organic matter content.


Author(s):  
V. А. Shevchenko ◽  
A. V. Nefedov ◽  
A. V. Ilinskiy ◽  
А. Е. Morozov

Long-term observations of the drained soil of peat-podzolic-gley light loam on ancient alluvial sands state on the example of the meliorative object "Tinky-2" showed that under the influence of agricultural use in the soil, the organic matter mineralization processes are accelerated. During the drainage process, the soil evolutionarily suffered the following changes: the peat layer was compacted, humified and mineralized, which was a reason of the transformation them into the humus horizon. Based on the monitoring studies results it was established that during 21 intensive use years the peat layer thickness was decreased by 74.5% and amounted to 5.51 inch, which in the following 20 years was decreased to a layer of 1.18 inch, and for another 14 years it became a homogeneous humus horizon containing difficulty identifiable plant remains. For half a century, the bulk density increased by 6 times and the total moisture capacity of the soil decreased by 3.6 times. Other indicators were changed significantly. So, the ash content by 2016 increased from 11.2% to 52.7%. It was a reason of the plough-layer decreasing and it mixes with the mineral sand horizon during plowing. It should also be noted that the total nitrogen content in the soil decreased by 1.13%, and total carbon by 15.3% from 1982 to 2016. The dynamics of changes in the soil acidity, phosphorus and potassium content is associated with the introduction of calcareous, organic and mineral fertilizers in the 1980s. The unsystematic exploitation of such soils leads to decrease in the agricultural products productivity and increase in energy costs. When planning these soils usage in agricultural production, it is necessary to develop and implement modern melioration technologies and techniques aimed to increase soil fertility.


2021 ◽  
Vol 13 (15) ◽  
pp. 8332
Author(s):  
Snežana Jakšić ◽  
Jordana Ninkov ◽  
Stanko Milić ◽  
Jovica Vasin ◽  
Milorad Živanov ◽  
...  

Topography-induced microclimate differences determine the local spatial variation of soil characteristics as topographic factors may play the most essential role in changing the climatic pattern. The aim of this study was to investigate the spatial distribution of soil organic carbon (SOC) with respect to the slope gradient and aspect, and to quantify their influence on SOC within different land use/cover classes. The study area is the Region of Niš in Serbia, which is characterized by complex topography with large variability in the spatial distribution of SOC. Soil samples at 0–30 cm and 30–60 cm were collected from different slope gradients and aspects in each of the three land use/cover classes. The results showed that the slope aspect significantly influenced the spatial distribution of SOC in the forest and vineyard soils, where N- and NW-facing soils had the highest level of organic carbon in the topsoil. There were no similar patterns in the uncultivated land. No significant differences were found in the subsoil. Organic carbon content was higher in the topsoil, regardless of the slope of the terrain. The mean SOC content in forest land decreased with increasing slope, but the difference was not statistically significant. In vineyards and uncultivated land, the SOC content was not predominantly determined by the slope gradient. No significant variations across slope gradients were found for all observed soil properties, except for available phosphorus and potassium. A positive correlation was observed between SOC and total nitrogen, clay, silt, and available phosphorus and potassium, while a negative correlation with coarse sand was detected. The slope aspect in relation to different land use/cover classes could provide an important reference for land management strategies in light of sustainable development.


2018 ◽  
Vol 64 (No. 9) ◽  
pp. 421-426
Author(s):  
Li Jun ◽  
Liu Lixin ◽  
Zhang Chunlei ◽  
Chen Chang ◽  
Lu Guangyuan ◽  
...  

Soil microbial properties are varied by growing different crops, ultimately reflecting the growth and reproduction of crops. In this study, two types of oilseed rape (Brassica napus L. ZS11 and ZY821) and wheat (Triticum aestivum L. ZM9023) were planted in the Jianghan plain of China. Rhizosphere soil samples were collected three months after sowing. Soil physicochemical properties, enzyme activities and microbial diversity were determined. The results showed that soil available phosphorus significantly increased from 25.57 mg/kg (ZM9023) to 33.20 mg/kg (ZS11) and 35.72 mg/kg (ZY821), respectively. Invertase activity of ZS821 (0.86 mg glucose/g) was significantly lower than in ZS11 (1.04 mg glucose/g). Acid phosphatase activity under planting rapes was significantly higher than that under wheat. Urease activities significantly increased from 40.88 mg NH<sub>4</sub><sup>+</sup>-N/g soil/24 h (NFP) to 49.04 mg NH<sub>4</sub><sup>+</sup>-N/g soil/24 h (FNP) and 51.28 mg NH<sub>4</sub><sup>+</sup>-N/g soil/24 h (ZM9023), 51.60 mg NH<sub>4</sub><sup>+</sup>-N/g soil/24 h (ZY821) and 52.28 mg NH<sub>4</sub><sup>+</sup>-N/g soil/24 h (ZS11), respectively. The ACE (abundance based coverage estimator) and Chao1 indexes of bacteria of ZS11 were lower than ZY821, which were similar to ZM9023. Fertilization increased soil bacterial ACE and Chao1 indexes. However, ACE and Chao1, Shannon and Simpson indexes of soil fungi for ZS11 were significantly higher than in ZY821, which were similar to ZM9023 (except for the Shannon index).


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chen Wang ◽  
Xuehong Ma ◽  
Gang Wang ◽  
Guitong Li ◽  
Kun Zhu

AbstractSoil O2 dynamics have significant influences on greenhouse gas emissions during soil management practice. In this study, we deployed O2-specific planar optodes to visualize spatiotemporal distribution of O2 in soils treated with biological soil disinfestation (BSD). This study aimed to reveal the role of anoxia development on emissions of N2O and CH4 from soil amended with crop residues during BSD period. The incorporation of crop residues includes wheat straw only, wheat straw with biochar and early straw incorporation. The anoxia in soil developed very fast within 3 days, while the O2 in headspace decreased much slower and it became anaerobic after 5 days, which was significantly affected by straw and biochar additions. The N2O emissions were positively correlated with soil hypoxic fraction. The CH4 emissions were not significant until the anoxia dominated in both soil and headspace. The co-application of biochar with straw delayed the anoxia development and extended the hypoxic area in soil, resulting in lower emissions of N2O and CH4. Those results highlight that the soil O2 dynamic was the key variable triggering the N2O and CH4 productions. Therefore, detailed information of soil O2 availability could be highly beneficial for optimizing the strategies of organic amendments incorporation in the BSD technique.


2019 ◽  
Vol 1 (1) ◽  
pp. 09-13
Author(s):  
Hakoomat Ali ◽  
Asad Abbas ◽  
Shabir Hussain ◽  
Shoukat Ali Abid ◽  
Shazia Khaliq ◽  
...  

Cotton is an important cash crop and source of foreign exchange. Nitrogen is a critical nutrient for plant growth throughout the life span of the crop. Wheat straw mulch not only source of nitrogen supply but also improves soil fertility and reduces soil erosion. The current study was performed to investigate the effects of mulches and nitrogen application on cotton productivity and fiber quality at the Central Cotton Research Institute (CCRI), Multan. Two crop residues i.e. wheat straw and non wheat straw were used in main plots while nitrogen levels viz. 0,50, 100 and 150 kg ha-1 were randomized in subplots. The highest seed cotton yield (22.99 t ha-1) was obtained by the combination of nitrogen fertilizer application highest level (150 kg N ha-1) along with the wheat straw (20.27 t ha-1). The fiber quality was also affected by the wheat straw along with nitrogen application 150 kg N ha-1 and gave maximum results. In conclusion, wheat straw along with 150 kg ha-1 of Nitrogen application gave maximum results on cotton production as compared to non straw with low nitrogen application.


Author(s):  
Vítězslav Vlček ◽  
Miroslav Pohanka

Soil samples (n = 11) were collected in the chernozem areas of the Czech Republic (the Central Europe) from the topsoil and used as representative samples. All sampling areas have been used for agricultural purposes (arable soil) and they were selected as typical representatives of agricultural soil. These samples represented the soil with same genesis (to reduction differencies between soil types) but with different soil properties (physical and chemical). Complete chemical and physical analyses were made for confirmation of copper adsorption on solid phase: we analysed the particle size distribution, content of oxidizable carbon (Cox), the cation exchange capacity (CEC), supply of exchange calcium, magnesium, sodium, phosphorus and potassium, soil reaction and the total supply of Fe, Al, Mn, Ca, Mg, K, P and N. The strongest simple correlation between analysed soil properties and copper concentration had content of available magnesium (r = 0.44) and available phosphorus (r = −0.51). In the case of multiple correlations (i. e. collective influence of multiple soil properties) had the strongest influence combination of clay, soil reaction, total content of phosphorus, available magnesium and available phosphorus. The main influence of phosphorus and magnesium is evident. We suppose that copper and phosphorus enter into specific complex. Influence of these five soil properties can explain 92.7 % (r = 0.927) changes in the content of copper changes in the experiment.


2015 ◽  
Vol 17 (1) ◽  
pp. 41-46 ◽  
Author(s):  
MA Haque ◽  
P Bala ◽  
AK Azad

A field experiment was conducted at the farm of Bangladesh Agricultural University, Mymensingh during November 2009 to March 2010 to study the response of three lentil varieties (viz., BARI Masur-1, BARI Masur-2 and BARI Masur-3) to Rhizobium inoculations to yield. There were three Rhizobium inoculants (Rhizobium strain BINA L4, Rhizobium strain TAL 640, and mixed culture) with uninoculated control and urea @ 50 kg ha-1. Phosphorus and potassium @ 26 kg P ha-1from TSP and 33 kg K ha-1 from MP were used as basal. It was observed that Rhizobium inoculation alone increased plant height, grain yield and crop residues yield of plant significantly compared to uninoculated control. Local inoculants BINA L4 performed better than the exotic culture TAL 640 in respect of yield. 50 kg urea ha-1 also recorded better results than control but not superior to any of the inoculation treatments. The highest seed (1,565 kg ha-1) and crop residue yields (3,303 kg ha-1) were recorded from the lentil variety BARIMasur-3 inoculated with mixed culture.Bangladesh Agron. J. 2014, 17(1): 41-46


2017 ◽  
Vol 17 (1) ◽  
pp. 19
Author(s):  
Tihomir Predić ◽  
Petra Nikić Nauth ◽  
Bojana Radanović ◽  
Alen Predić

This paper presents the results of the research of soil contamination in flooded area located at the confluence of Bosna and Sava rivers. The sampling was performed according to a network of points Etrs89 reference European Network based on grid points of 500 m x 500 m. The total number of soil samples was 31 and 13 samples of sediment-mud. In the soil samples and sediment-mud samples various parameters were analyzed: pH H2O and 1 M KCl, humus (method by Tjurin), and the readily available phosphorus and potassium (AL method). The interpretation of total content of heavy metals in the soil was conducted according to Dutch Soil Remediation Circular (2009). The samples were analyzed for the total content of heavy metals: Pb, Cd, Cr, Ni, Zn, Cu (aqua regia digestion, AAS) and the elevated concentrations of Ni. However, Ni content in mud was from 240.5 mg/kg to 294.5 mg/kg, in soil it was from 128.9 mg/kg to 452.1 mg/kg. All mud samples were alkaline, and 79% of soil samples were alkaline and of neutral reaction. The analysis indicated that the flood in May 2014 was not caused by soil contamination with heavy metals, which however contributed to the increase of the overall content of these elements in the soil.


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