scholarly journals Experimental study on the expression and diagnostic significance of I-FABP in acute intestinal ischemia

2021 ◽  
Vol 5 (2.1) ◽  
pp. 30
Author(s):  
Haikun Li ◽  
Minhua Wang ◽  
Xiansen Zhu ◽  
Xiaoqing Zhou ◽  
Bin Yang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Early Diagnosis ◽  
Intestinal Mucosa ◽  
Intestinal Ischemia ◽  
Control Group ◽  
Direct Immunofluorescence ◽  
Intestinal Tissue ◽  
Acute Intestinal Ischemia ◽  
The Difference ◽  
Experimental Group

Objective: To detect the expression and distribution of I-FABP in intestinal tissue and the changes of serum concentrations at different time of acute intestinal ischemia, and explore the significance and mechanism of I-FABP in early diagnosis of acute ischemic bowel disease. Methods: The selected 96 healthy adult SD rats were randomly divided into the experimental group and control group; 48 in each group. Each group was randomly subdivided into 6 groups with 8 rats in each group. The superior mesenteric artery was ligated in the experimental group and the peritoneal switch operation was performed in the control group. The venous blood samples were extracted from each group rats’ right ventricle at 0.5 h, 1 h, 2 h, 4 h, 8 h, 12 h after the operation and the concentration of I-FABP was tested respectively. Then the rats were killed, and the diseased intestinal tubes were cut out for paraffin sections. The I-FABP in intestinal tissue was stained by routine HE staining and direct immunofluorescence staining. Results: The I-FABP was mainly expressed in the epithelial villi of intestinal mucosa, and there was a small amount of expression in the intestinal submucosa and even the muscularis. Within 1 hour of intestinal ischemia, the number of I-FABP positive granules in the intestine and intestinal cavity increased gradually, and then gradually decreased after 1 hour. The difference has statistically significant between the experimental group and the control group (P < 0.05). The serum I-FABP: In the experimental group, the serum I-FABP concentration began to increase at 0.5 h, and reached a peak at 1 h (290. 24 ± 156.69) μg·L–1, then gradually decreased. Compared with the control group, the difference was statistically significant (P < 0.05). Conclusion: I-FABP usually mainly exists in the epithelial cells of intestinal mucosa. When acute intestinal ischemia occurs, the epithelial cells of intestinal mucosa permeability changes; I-FABP expression rapidly releases to intestinal tissue and intestinal cavity, and is absorbed into the blood. Therefore, I-FABP has certain clinical significance in early diagnosis and treatment of acute intestinal ischemia.

Study on the Effect of Composite Nanoparticles on Corneal Epithelial Cell Immune Mechanism Based on Dectin-1 Signaling Pathway

2021 ◽  
Vol 21 (2) ◽  
pp. 1148-1153
Author(s):  
Ming Li ◽  
Juan Liu
Keyword(s):  
Epithelial Cells ◽  
Signaling Pathway ◽  
Composite Nanoparticles ◽  
Control Group ◽  
Corneal Epithelial Cells ◽  
Blocking Group ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cells ◽  
The Difference ◽  
Experimental Group

In order to investigate the role of composite nanoparticles in the immune stage, we observed the expression of R-1 in anti-mycotic infection of human corneal epithelial cells, and divided the experiment into control group, fungal stimulation group, Gw507 blocking group and Dectin-1. In the inhibitor group, the immune effect of human corneal epithelial cells was studied. Subsequently, the expression of total R-1 and phosphorylated form of p-R-1 in each experimental group was detected by western blot method. Finally, real-time quantitative PCR was used to detect the expression of factors IL-6 and IL-8. We concluded that p-R-1 was slightly expressed in the control group. After 15 minutes of fungal stimulation, the expression level in the experimental group was significantly higher than that in the blank group, and the difference was statistically significant (p < 0.05). In the blocking group of two different inhibitors, the expression of p-R-1 was lower than that in the fungal stimulation experimental group, and the difference was statistically significant (p < 0.05). Therefore, R-1 is expressed in corneal epithelial cells and the fungus exerts its antifungal effect through the Dectin-1 signaling pathway.

scholarly journals QOLP-17. GROUP MEDICAL PLAY EFFECTIVELY IMPROVES HOSPITALIZATIONADAPTABILITY OF PEDIATRIC PATIENTS WITH NEUROTUMOR

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii178-ii178
Author(s):  
Xing Zhang ◽  
Fuqiang Zhang ◽  
Mingyao Lai ◽  
Juan Li ◽  
Yangqiong Zhang ◽  
...  
Keyword(s):  
Social Status ◽  
Pediatric Patients ◽  
Picture Book ◽  
Family Satisfaction ◽  
Control Group ◽  
Play Intervention ◽  
Significant Difference ◽  
The Difference ◽  
Therapeutic Play ◽  
Experimental Group

Abstract OBJECTIVE To explore the effect of group medical games on the hospitalization adaptability of pediatric patients with neuro tumor. METHODS pediatric patients with neuro tumor (age:6 to 13 years) who were treated in hospital from June to December 2019 and were hospitalized for 1 month to 2 months. 29 pediatric patients(mean age:9y) were selected as the control group and treated as usual; 26 pediatric patients(meanage:8y) were selected as the experimental group for group therapeutic play intervention. Interventions last Monday, Wednesday and Friday of each week, with an average duration of one hour. Group medical play include: medical picture book education, medical preview game, emotional games, social table games. Two groups completed self-made questionnaires at the time of admission and two weeks after admission, including: diet, sleep, compliance, and social status, hospital adaptation and other related issues, two groups completed a satisfaction questionnaire after two weeks of admission, recorded analysis and compared the difference of questionnaire data and satisfaction of the two groups of pediatric patients. RESULTS There was no statistical difference in age and sex between the two groups, and there was no significant difference in baseline RESULTS: The re-test results showed that the experimental group was significantly better than the control group in terms of social status, hospital adaptation, compliance and family satisfaction(p＜0.05). CONCLUSION Group medical games can effectively improve the adaptability, compliance and family satisfaction of pediatric with neuro tumor.

Influence of Tobacco on Physical Fitness Recovery of College Students After Sports

2021 ◽  
Vol 7 (5) ◽  
pp. 3445-3451
Author(s):  
Chen Yake
Keyword(s):  
College Students ◽  
Physical Fitness ◽  
Aerobic Exercise ◽  
Negative Impact ◽  
Control Group ◽  
Exercise Time ◽  
Activity Intensity ◽  
Group I ◽  
The Difference ◽  
Experimental Group

Objectives: In this paper, the effects of tobacco on aerobic exercise ability and physical fitness recovery of college students were studied. Methods: University group sports intervention form: traditional characteristic project (basketball) + Taiji soft ball (R&D intervention project). Exercise time: 3 times/week; Activity duration: 30min; Activity intensity: the heart rate is controlled at 120-140 beats/min. All the college students in the experimental group are students who have never smoked, and the college students in the control group are students who have smoked for more than two years. The other conditions are the same. Results: The exercise time and endurance of experimental groups I and II were significantly lower than those of the control group, and the cardiopulmonary function was significantly lower than that of the control group. The indexes of experimental group II changed significantly compared with experimental group I, and the difference was statistically significant. Conclusion: Cigarette smoke can significantly reduce the aerobic exercise ability and anti fatigue ability. The longer the smoking time, the more serious the adverse effects. Therefore, tobacco smoke and nicotine will damage college students’ aerobic exercise ability and have a negative impact on the recovery of physical fitness after exercise.

scholarly journals The Effectiveness of the Use of Animation in Arabic Language Learning

2017 ◽  
Vol 13 (10) ◽  
pp. 124 ◽  
Author(s):  
Norhayati Che Hat ◽  
Mohd Fauzi Abdul Hamid ◽  
Shaferul Hafes Sha'ari ◽  
Safawati Basirah Zaid
Keyword(s):  
Student Achievement ◽  
Language Learning ◽  
Language Teaching ◽  
Arabic Language ◽  
Control Group ◽  
Teaching Aid ◽  
Significant Difference ◽  
Post Test ◽  
The Difference ◽  
Experimental Group

Implementation of animation as an Arabic language teaching aid is an innovation in creating an atmosphere that can influence student achievement. This study aimed to identify the effectiveness of the use of animation in Arabic language teaching and learning among diploma students at Universiti Sultan Zainal Abidin (UniSZA), Terengganu, Malaysia. A total of 66 diploma students were randomly selected and divided into experimental group (n = 33) and control group (n = 33). The results obtained from the data collected from pre-and post-test for each group were analyzed using t-test in SPSS version 17.0. The results showed a significant difference of (t = 8789, df = 64, p <0.05) between the achievement of the experimental group and the control group in the post test. The difference in mean score of the experimental group and the control group was 33.03. This shows that there is significant improvement in Arabic language according to the groups. The difference prove that the use of animation in learning sessions contribute to the achievement of students in the Arabic language. This study advocate the idea that animation applications can be integrated as part of language teaching aid to positively improve student achievement, classroom learning environment and student motivation. 

scholarly journals PENGARUH MODEL COOPERATIVE LEARNING TIPE STUDENT TEAMS ACHIEVEMENT DIVISION TERHADAP KETERAMPILAN LAY-UP SHOOT BOLA BASKET

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Davi Sofyan
Keyword(s):  
Cooperative Learning ◽  
Sampling Technique ◽  
Control Group ◽  
Student Teams ◽  
Post Test ◽  
Quasi Experimental ◽  
The Mean ◽  
The Difference ◽  
Experimental Group ◽  
Cluster Random Sampling

The purpose of this study was to determine how much influence the cooperative learning model type student team's achievement division has on basketball shoot lay-up skills. The research method used in this research is a quasi-experimental method. The type of research design used was the non-equivalent group design. The population in this study were students of class XI SMA Negeri 1 Majalengka in 2019 totaling 424 students. The sampling technique used in this study was cluster random sampling technique. The sample in this study amounted to 214 students consisting of class XI IPS 1-4 and XI MIPA 7-8. The experimental group class XI IPS 1-2 and XI MIPA, control group class XI IPS 3-4 and XI MIPA 8. The results showed that the mean pre-test lay-up shoot of the experimental group was 4.09 while the control group was 3.92. The mean post-test shoot lay-up of the experimental group was 6.76 while the control group was 4.72. Furthermore, by looking at 95% confidence interval of the difference, there is a difference between the shoot lay-up results of the experimental group and the control group, which is -0.190 to 2.412. Thus, it can be concluded that STAD is effective in improving basketball shoot lay-up skills.

scholarly journals Improvement of Physical, Honesty, Discipline and Cooperation in Class IV Elementary School Students through Circuit Training Learning Model

2020 ◽  
Vol 3 (1) ◽  
pp. 244-250
Author(s):  
Dean Novianti ◽  
I Made Sriundy Mahardika ◽  
Abdul Rachman Tuasikal
Keyword(s):  
Elementary School ◽  
Physical Fitness ◽  
Learning Model ◽  
Control Group ◽  
Circuit Training ◽  
School Students ◽  
Post Test ◽  
The Difference ◽  
Efficient Learning ◽  
Experimental Group

Physical education program for sport and health allocates four lesson hour of which each hour consists of 35 minutes in one week. It requires an innovative and efficient learning model in order to improve physical fitness, honesty, discipline, and cooperation. The researcher developes a circuit training learning model in the from circuit games which is a modified form of circuit training. The study aim to examine the effectiveness of the circuit training learning model in improving physical fitness, honesty, discipline, and cooperation in students of Elementary School Sidoarjo. The type of research used is quantitative and qualitative by using research and development method, and data analysis using ANOVA. The data collection process is carried out by pre-test and post-test and then the results of the research data are analyzed using SPSS series 2.0. The results of the study shows (1) the sig value of 0,011 smaller than the 0,05 ( p< 0,05 ), meaning that there is the difference in the results of the TKJ I test on groups of students who are not treated compared to the group of students who are given the learning model. (2) the average group of students who are not given the treatment (control group) is 12,50 while for the group of students         given treatment (experimental group) is 15,15, meaning that there is an increase in test results againts the experimental group at 45%, (4) there is an increase in the observations of the discipline attitude from the control group compared to the experimental group at 35 %, (5) there is an increase in the observations of the discipline attitudes of the control group compared to the experimental group at 55%. Based on the above analysis it can be cocluded that the circuit training learning model can improve physical fitness and cultivate honesty, discipline and cooperattion in students of Elementary School.  

scholarly journals The Effectiveness of Input on Voiced and Unvoiced Consonant Sounds in a Native Arabic Speaking Context: An Action Research Project

2020 ◽  
Vol 2 (3) ◽  
pp. p1
Author(s):  
Gareth Morgan
Keyword(s):  
Action Research ◽  
Control Group ◽  
Action Research Project ◽  
Research Project ◽  
English Speaking ◽  
The Difference ◽  
Arabic Speaking ◽  
Consonant Sounds ◽  
Positive Effect ◽  
Experimental Group

The paper describes the process and outcomes of an action research project with the aim of determining whether focusing classroom input on voiced and unvoiced consonant sounds has a positive effect on their production. Statistics were derived from English-speaking respondents listening to native Arabic speaking participants from an experimental group, who had received input on the difference between these sounds, and practiced their production, as well as to speakers from a control group who had received neither input nor practice. The rates of intelligibility were compared, with the conclusion being that the provision of limited input on this pronunciation issue does not, generally speaking, result in the ability to produce the sounds with greater clarity.

scholarly journals SLAUGHTER QUALITIES AND BIOLOGICAL VALUE OF RABBIT MEAT FOR FEEDING VITAMIN-MINERAL SUPPLEMENT «TEKRO»

2020 ◽  
Vol 11 (4) ◽  
pp. 70-80
Author(s):  
M. M. Fedorchenko ◽  
V. V. Malina ◽  
V. A. Hryshko
Keyword(s):  
Control Group ◽  
Internal Organs ◽  
Mineral Supplement ◽  
Biological Value ◽  
Mineral Supplements ◽  
The Difference ◽  
Rabbit Meat ◽  
Meat Samples ◽  
Experimental Group

The problem of complete mineral and vitamin nutrition in rabbit breeding is relevant today, despite new discoveries, and research in this direction continues both in Ukraine and around the world. The paper presents experimental data from the results of research to study the effect of different doses of vitamin and mineral supplement "Tekro" in the diets of young rabbits on their productivity, slaughter quality and biological value of rabbit meat. The obtained data of body growth, massometric indicators of carcasses and internal organs of rabbits of experimental groups after slaughter indicate a positive effect of vitamin-mineral supplement "Tekro" on the intensity of development of the body and individual internal organs, which contributes to increased metabolic processes and weight gain in animals research groups. Feeding vitamin and mineral supplements to rabbits of experimental groups of the New Zealand breed for 45 days contributed to a better transformation of feed nutrients into products. In rabbits of the 3rd experimental group, which together with basic ration were fed 3.5 % of the premix "Tekro" at 90 days of age, the highest rates of body weight were recorded, which differed by 8.89 % compared to the control group. In a set of indicators that characterize the nutritional value of rabbit meat, we studied sensory indicators that characterize the quality of rabbit meat: taste, color, pH, juiciness, moisture content, elasticity, aroma, "marbling", etc. It was found that in the appearance of carcasses and muscles in the control group, the samples scored 8.4 points. In the 3-rd and 4-th experimental groups, the number of points on this indicator was higher by 1.2 %. The difference was a trend. Examining the aroma of meat, it was found that in the control of this indicator, the samples scored 7.8 points. In meat samples from experimental groups 3 and 4, the aroma was more identical to natural, so the samples received 2.6 % higher scores. Samples from 3 experimental groups scored the most points in terms of taste. Compared with the control, the figure was higher by 3.6 %. Examining the juiciness of rabbit meat, it was found that samples obtained from carcasses 2 and 3 of the experimental group tended to increase this indicator. Summing up the data, it was found that the highest overall score was found in meat samples from 3 experimental groups. The difference with the control was within the trend and amounted to 2.4 %. Feeding rabbits vitamin and mineral supplements to rabbits helps to obtain good quality meat products with high culinary properties. No data were available to support the effect of vitamin and mineral supplements on compound feed on the synthesis of toxic substances in muscle tissue or the deterioration of rabbit meat. The biological value of muscle samples from rabbits of the 2-nd experimental group did not differ from the control data. The increase was only 0.11 %. It was found that the biological value of muscle tissue from animals of the 4th experimental group was greater than in the control. The difference was 2.6 % Meat obtained from rabbits of the 3rd experimental group, which were fed compound feed with a content of mineral-vitamin supplement "Tekro" 3.5 %, in biological value outweighed the control indicators. The difference was not significant and amounted to 4.1 %. The use of different doses of vitamin and mineral supplements in the feed of young rabbits did not lead to significant changes in the quality of raw hides and furs.

scholarly journals Influence of Network Multimedia Nutritional Supplements on Basketball Exercise Fatigue Based on Embedded Microprocessor

2022 ◽  
Vol 2022 ◽  
pp. 1-13
Author(s):  
Qiao Chen ◽  
Shihong Liu
Keyword(s):  
Hemoglobin Concentration ◽  
Nutritional Supplements ◽  
Weight Lifting ◽  
The Body ◽  
Control Group ◽  
Energy Materials ◽  
Long Distance ◽  
Basketball Players ◽  
The Difference ◽  
Experimental Group

Sports can cause the consumption of energy materials in the body. The rational use of nutritional supplements can maintain the homeostasis of the organism, which plays a very important role in improving the competitive performance of sports athletes. The purpose of this study is to explore the effect of nutritional supplements on basketball sports fatigue. The method of this study is as follows: first of all, 15 basketball players in our city were selected as the experimental objects, and they were randomly divided into the experimental group and the control group. The members of the experimental group took nutrients. After the training, 6 days a week, 3 hours in the morning and 3 hours in the afternoon, and the rest was adjusted on Sunday. Before training, four weeks and eight weeks of training, the blood routine indexes and body functions of athletes were tested. The results showed that the number of red blood cells, hemoglobin concentration, and average hemoglobin concentration of ligustilide supplement of the athletes were at the level of 0.05 after 4 weeks and 8 weeks, and the difference was significant ( P < 0.05 ). The nutritional supplements were used in sprint (3.4 s less), long-distance running (12.8 s less), and weight lifting (6.2 kg more) to a certain extent. Nutritional supplements are used as an auxiliary means of diet to supplement the amino acids, trace elements, vitamins, minerals, etc. required by the human body. The conclusion is that nutrition supplement can effectively improve the indexes of athletes’ body in about four weeks, but the effect is not obvious after a long time. This study provides a certain method for the research of nutritional supplements in the field of sports.

scholarly journals Application of Magnetic Resonance Image Intelligent Data Acquisition in Ovarian Cancer (Preprint)

2020 ◽  
Author(s):  
Tingting Liang ◽  
Yanlei Dong ◽  
Xinrui Zhao ◽  
Lu Wang ◽  
Hui Xu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Magnetic Resonance ◽  
Epithelial Cells ◽  
Data Acquisition ◽  
Cell Lines ◽  
P53 Protein ◽  
Control Group ◽  
Ovarian Epithelial Cells ◽  
Intelligent Data Acquisition ◽  
Experimental Group

UNSTRUCTURED As a diagnostic method with no radiation, high resolution of soft tissue, and different imaging methods, Magnetic Resonance Imaging (MRI) intelligent data acquisition is more and more widely used in the examination of abdominal, pelvic, and other organ lesions. In order to study the diagnostic effect of multi-mode magnetic resonance intelligent data acquisition on ovarian cancer and the ovarian cancer model modified based on p53-/-+Myc+ASAP1 gene, NSG mice were selected as experimental subjects in this study. 293FT cell lines packaging p53, Myc, and ASAP1(ArfGAP with SH3 domain, Ankyrin repeat and PH domain 1) recombinant lentivirus were inoculated into mouse ovarian epithelial cells to construct mouse ovarian epithelial cell tumor cell lines and their performance was analyzed. According to the different injection cell lines, they were divided into the experimental group and the control group. Tumor samples were collected and the mice were analyzed using immunofluorescence staining and MRI. The results showed that, in the detection of protein expression in genetically modified cell lines, for p53-/-+Myc+ASAP1 fully modified cell lines, the high expression of ASAP1 and Myc functional proteins was detected after the lentivirus containing p53-/-, ASAP1, and Myc were introduced into mouse ovarian epithelial cells, while the expression of p53 protein decreased significantly; after inoculation into mice, it was found that the expression of ASAP1 protein and Myc protein in the experimental group was significantly higher than that in the control group, while the expression of p53 protein in the experimental group was significantly lower than that in the control group, with significant statistical difference; further MRI diagnosis of two groups of mice showed that the ADC (Apparent dispersion coefficient) value of the experimental group was significantly higher than the control group, there were statistically significant differences. Therefore, it was found that p53 gene expression was down-regulated and Myc and ASAPl genes were overexpressed in the tumor tissues and tumor cells formed, and tumor formation differences between the two groups of mice could be obviously found after MRI intelligent data acquisition, which provided experimental basis for early diagnosis of breast cancer in the later clinical stage.

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