scholarly journals The content of fagopyrin and polyphenols in common and tartary buckwheat sprouts

2013 ◽  
Vol 63 (4) ◽  
pp. 553-560 ◽  
Author(s):  
Samo Kreft ◽  
Damjan Janeš ◽  
Ivan Kreft

Abstract Dried buckwheat herb is used in medicinal products whereas fresh green plant parts, especially sprouts, are consumed as a vegetable. The herb contains fagopyrins, which cause sensitivity to light after ingestion. The aim of this study was to investigate the impact of different growing conditions and the development phase on the content of fagopyrin and phenolic compounds in buckwheat sprouts. Total flavonoid and total phenol contents, fagopyrin content and antioxidant activity were determined spectrophotometrically. Fagopyrin and flavonoids were located almost exclusively in cotyledons. Based on a comparison to hypericin toxicity, the recommendable intake of buckwheat sprouts was estimated to be less than 40 g per day.

2018 ◽  
Vol 101 (6) ◽  
pp. 1788-1793 ◽  
Author(s):  
Shahla Shameh ◽  
Bahman Hosseini ◽  
Abolfazl Alirezalu ◽  
Ramin Maleki

Abstract Background: The petals of Rosa species are used in the food industry and various traditional medicinal products, but few studies exist on the phytochemical composition and antioxidant activity of petals of Rosa L. species grown in Iran. Objective: Phytochemical characteristics and antioxidant activity and some phenolic compounds of petals of six Rosa L. species were studied. Methods: Total phenolic content, total flavonoid content, and antioxidant activity were determined using Folin–Ciocalteu reagent, aluminum chloride method, ferric-reducing antioxidant power (FRAP), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays, respectively. An HPLC system was used for quantitative analysis of phytochemical compounds. Hierarchical cluster analysis (HCA) and principal component analysis (PCA) were performed among the variables analyzed using Minitab software. Also, heat maps were used to visualize phytochemical characteristics and antioxidant activity in each species using GraphPad Prism software. Results: The amount of total phenol content, total flavonoid content, and antioxidant activity were in the range of 25.13–52.01 mg gallic acid equivalents/g dry weight (DW), 0.61–0.82 mg quercetin equivalents/g DW, 11.47–20.93 µmol Fe++/g DW (FRAP), and 31.66–74.44% (DPPH), respectively. The p-coumaric acid (647.28 μg/g DW) and chlorogenic acid (24.37–135.23 μg/g DW) were found to be the most abundant phenolic compounds in the extracts of rose petals. The HCA and PCA revealed three distinct categories of species based on phytochemical composition and antioxidant activity. Conclusions: These results showed that phytochemical characteristics of different rose species widely correlated with species type and are promising sources of natural antioxidants beneficial for use in the food or pharmaceutical industries. Highlights: Iran is one of the main centers for genetic diversity of Rosa L. The petals of Rosa species are used in the food industry and various traditional medicinal products, but few studies exist on the phytochemical composition and antioxidant activity of petals of Rosa L. species grown in Iran. Antioxidant activity and phytochemical compound of Six Rosa L. species petals grown in Iran were studied. Phenolic compounds in petals of Rosa were analyzed by HPLC. The color parameters, amount of total phenolic, total flavonoids, antioxidant activity and some individual phenolic compounds were significantly variable amongst Rosa species.


2018 ◽  
Vol 6 (3) ◽  
pp. 807-815
Author(s):  
OMOTOSHO OMOLOLA ELIZABETH ◽  
IHEAGWAM FRANKLYN NONSO ◽  
NOIKI IFEOLUWA ADEBOLA ◽  
OMINI JOY JOHN

Annonamuricata plant parts possess a broad range of medicinal and biological properties. This research compared the chemical composition and antioxidant properties of Annonamuric at aparts. Proximate, mineral, total phenol and total flavonoid content as well as invitro antioxidant activity were examined. Results revealed the leaves contained significantly(p<0.05) higher composition of moisture (8.69±0.22%), ash (4.60±0.02%), protein (14.53±0.11%), crude fat (10.28±0.03%),chromium (0.38±0.05 mg/100g), nickel (1.75±0.04mg/100g), total phenol (1.01±0.03mgpyrocatechol/mL) and total flavonoid (1.12±0.03mgGAE/mL)compared to the respective values for root. Carbohydrate (9.29±0.24%), lead (0.13±0.02mg/100g) and cobalt (1.93±0.02mg/100g) composition was significantly lower(p<0.05) in the leaves compared to the respective compositions in the root. The leaf and root extract exhibited a concentration-dependent increase in hydroxyl radical scavenging activity with no observable(p<0.05)difference in their EC50 value. This study suggests the leaves of A. muricata found in Covenant University had better chemical composition when compared to the root. Nonetheless, these plant parts may be further exploited for not only their nutritive composition and mineral content but also a natural source of antioxidant agents.


2002 ◽  
Vol 31 (2) ◽  
pp. 95-105 ◽  
Author(s):  
V.T. Sundaramurthy

The dynamics of insect pests and the impact of the management system on commercial village farms were investigated over several years. Damage to the cotton crop by bollworms in the polycrop system increased with the advancement of crop growth, with greater application of nitrogenous fertilizers and irrigation and with reduced planting distances between the conventional varieties. The activity of the adults of Helicoverpa armigera, Spodoptera litura and Pectinophora gossypiella in this crop system was at its peak during the middle reproductive phase of the cotton crop, and subsequently declined. The foliaceous, tall growing hybrids and varieties with heavy canopies had greater infestations of the adults of the leaf-feeding insect, S. litura. Investigations carried out over several years on commercial village farms show that the insect management system was lethal to the insect complex in the cotton system. De-topping of the growing terminals of plants greatly reduced oviposition by insecticide-resistant populations of H. armigera. The manual collection of larvae and affected plant parts resulted in the reduction of bollworm infestation on the village farms by 3–90%. Liberation of Trichogramma achea in great numbers reduced the population of different species of bollworms on the village farms. This system has reduced environmental pollution with insecticides by 35–59% and contamination of labourers' and farmers' blood and female workers' breast milk by 35–76%. Increased productivity of varieties and hybrids has been maintained over an observed range (according to growing conditions) from 870 to 2,220 kg/ha of seed cotton. The population of native natural enemies of cotton insects was increased several fold and had inverse density-dependent effects on the host insects. The pest management system has been adopted in cotton-growing states with financial assistance from the government of India since 2000.


Antioxidants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 356 ◽  
Author(s):  
Sachadyn-Król ◽  
Materska ◽  
Chilczuk

The effect of treatment of pepper fruits with gaseous ozone and storage time following the ozonation process on changes in the content of lipophilic fraction is analyzed for the first time in this paper. The aim of the present study was to assess the impact of ozone treatment on the composition of lipophilic compound fraction and its antioxidant activity (AA). Pepper fruits of cv. Cyklon were ozonated for 1 and 3 h immediately after harvesting. Then, the fruits were stored for 30 days under refrigeration conditions. The total content of phenolic compounds and the AA of the lipophilic fraction isolated from the pericarp and placenta of the fruits were investigated after 10, 20, and 30 days of storage. Additionally, quantitative high-performance liquid chromatography diode array detection analysis of individual phenolic compounds was performed. The results revealed that the content and activity of secondary metabolites varied during storage, with the highest values recorded on the 20th day after harvest, both in control and ozonated fruits, regardless of the ozone dosage used. Treatment of the fruits with ozone for 3 h, but not for 1 h, exhibited a positive effect on the phenolic composition and AA during the prolonged storage of pepper fruits. Three hours of ozonation seems to be the appropriate time to increase the persistence of pepper fruits during storage.


2012 ◽  
Vol 81 (2) ◽  
pp. 117-122 ◽  
Author(s):  
Milan S. Stankovic ◽  
Neda Niciforovic ◽  
Vladimir Mihailovic ◽  
Marina Topuzovic ◽  
Slavica Solujic

Total phenolic content, concentration of flavonoids and in vitro antioxidant activity of twenty different extracts from the whole plant and plant parts (leaves, flowers and stems) of <em>Teucrium polium </em>were determined. The total phenolic contents ranged between 14.57 to 157.84 mg of GaA/g of extract. The concentrations of flavonoids varied from 6.48 to 139.87 mg of Ru/g of extract. Antioxidant activity was determined in vitro using DPPH reagent and expressed as concentration of each extract required to inhibit radical by 50% (<em>IC</em><sub>50</sub>) values that ranged from 26.30 to 2190.75 µg/ml. The methanolic leaves extract contain the greatest concentration of phenolic compounds (157.84 mg of GaA/g) and showed strong antioxidant activity (<em>IC</em><sub>50</sub> = 26.30 µg/ml). Ginkgo and Green tea extracts were analyzed for comparison, and the results indicated that some extracts of <em>T. polium </em>were equal in activity with Ginkgo or Green tea and some appeared to have greater activity. The obtained results suggest strong antioxidant activity and large contribution of separate analysis for the maximum exploitation of active phenolic compounds from <em>T. polium</em>. Based on this information, plant parts of this plant are natural sources of antioxidant substances of high importance.


2020 ◽  
Vol 8 (Spl-2-AABAS) ◽  
pp. S327-S335
Author(s):  
Alexander Loenidovich Mikhailov ◽  
◽  
Olga Arnoldovna Timofeeva ◽  
UlianaAleksandrovna Ogorodnova ◽  
Nikita Sergeevich Stepanov ◽  
...  

The current study was carried out to study the effect of growing conditions on the pharmaceutically promising biologically active phenolic compounds on the meadow clover plants. The impact of the plant development site on the contemplated mixes was additionally explored. The estimation of phenolic compounds in raw plant materials was carried out with the help of a spectrophotometer and photocolorimeter. Results of the study revealed that the place of plant growth conditions have remarkable effect on the content of the phenolic compounds. Further, the most ideal conditions available for clover plant growth and higher phenolic compounds were reported from the broad-leaved forests. Furthermore, the results also suggested that the most important elements which affecting the aggregation of phenolic compounds are soil nitrogen.


Author(s):  
GRACE SEREPINA SARAGIH ◽  
SISWADI SISWADI

Objective: Sterculia quadrifida R. Br. of Sterculiaceae family is locally known as “Faloak” in East Nusa Tenggara Province, Indonesia. S. quadrifida is used in folk medicine to treat hepatitis, rheumatism, and to recover stamina. The aim of this study was to determine the total flavonoids, total phenolic content (TPC), and antioxidant activity of extracts from different plant parts of S. quadrifida. Methods: The sampled parts of S. quadrifida were non-stripped stem bark, new regrown stem bark, old regrown stem bark, root bark, branch bark, and leaves. Stem bark was classified into three categories, namely, bark that has never been peeled (non-stripped stem bark), old regrown stem bark (estimated to be >6 months after debarking), and new regrown stem bark (estimated to be <6 months after debarking). Total flavonoid content (TFC) was determined by colorimetric aluminum chloride method and TPC was measured using Folin–Ciocalteu’s reagent. Antioxidant activity was determined with 2,2-diphenyl-1-picrylhydrazyl (DPPH). Results: The concentrations of flavonoids in S. quadrifida extracts from different plant parts varied from 0.58±0.13 to 1.25±0.10 mg QE/g. The TPC in the extracts of different plant parts ranged between 8.61±0.09 and 10.43±0.08 mg GAE/g. Branch bark has the highest total flavonoid and phenolic content. The extract of new regrown stem bark exhibited potent antioxidant activity with inhibitory concentration (IC50) values of 2.51±0.03 μg/ml. Conclusion: This study demonstrated for the first time that extracts from different plant parts of S. quadrifida exhibited strong antioxidant activity. However, the total phenolic and flavonoid contents in S. quadrifida only indicated a weak correlation with its antioxidant activity.


2021 ◽  
Vol 42 (5) ◽  
pp. 1347-1354
Author(s):  
M.K. Berwal ◽  
◽  
S.M. Haldhar ◽  
C. Ram ◽  
P.L. Saroj ◽  
...  

Aim: The aim of the study was Identification and quantification of phenolic compounds, total flavonoides content and antioxidant potential of flower buds, foliages, bark and root of a perennial herb, Calligonum polygonoides (Phog) of Thar Desert. Methodology: The methanolic extracts of different plant parts of C. polygonoides were subjected to LC-MS/MS (Waters Acquity UPLC-PDA, TQD) analysis for phenolic identification and spectrophotometric assay of total phenolic and flavonoids content and total antioxidant activity was estimated. Results: A total of 15 phenolic compounds were identified and quantified, among which gallic acid content was abundant, followed by catechin. Besides these, the other major phenolic compounds detected in different plant parts were vanillic, chlorogenic acid, epicatechin, coumeric acid, catechol, vanillic acid, epicatechin and syringic acid. Extremely higher values were recorded for total phenolics, flavonoids and antioxidant capacity. The values for phenol, flavonoids and total antioxidant activity in bark extracts were higher than that of clove extract. Interpretation: The identified phenolic compounds possess high antioxidant potential. These results provide scientific evidence for use of C. polygonoides as safe natural antioxidant compounds in nutraceutical and pharmaceutical industry.


2019 ◽  
Vol 6 (1) ◽  
pp. 79-90 ◽  
Author(s):  
Imen Laib ◽  
Fairouz Boubrik ◽  
Malika Barkat

Abstract The main objectives of this work are to optimize the extraction parameters, to test the antioxidant activity of Aloe Vera extract and to study the impact of this extract on deteriorating molds of Algerian variety of wheat (CIRTA). The extraction was optimized by central composite design. Determination of the polyphenols, flavonoids, and proanthocyanidins was performed by using colorimetric assays. Identification and quantification of phenolic compounds were performed by RPHPLC-UV method. The antioxidant activity was tested by three methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,20-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and CUPRAC (Cupric reducing antioxidant capacity), the antifungal activity of Aloe Vera extract on isolated strains from durum wheat were tested by dilution in a solid medium method. The optimum of total phenolic got was1,044 x 104 µg GAE/g of dry extract. The extract is rich in polyphenols, flavonoids, and proanthocyanidins. The analysis of phenolic compounds of Aloe Vera by RP-HPLC-UV revealed seven phenolic compounds. Strong antioxidant activity was obtained for Aloe Vera extract. Purification and microscopic study of isolated strains gave the possibility of identifying four strains: Alternaria spp1, Alternaria spp2, Penicillium spp, and Aspergillus spp. the antifungal potential of Aloe Veravaries according to the fungal genera and the concentrations of extract used.


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