scholarly journals Antimicrobial activity of Capparis spinosa as its usages in traditional medicine

2014 ◽  
Vol 60 (1) ◽  
pp. 39-48 ◽  
Author(s):  
Mohaddese Mahboubi ◽  
Atefeh Mahboubi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Traditional Medicine ◽  
Aqueous Extract ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Traditional Uses ◽  
Capparis Spinosa ◽  
Bacteria And Fungi

Summary Capparis spinosa roots and fruits are traditionally used for the treatment of diseases such as diarrhea and hemorrhoids. In the 10th century, farmers also used aqueous extract from its roots for disinfecting their plants’ seeds. Due to widespread use of this plant in traditional medicine, we evaluated different extracts (aqueous, methanol, ethanol and ethyl acetate) of fruits and roots of this plant against bacteria and fungi causing infections in plants or humans. The total phenolic and flavonoid content of extracts from fruits and roots and the antioxidant activity were evaluated. The antimicrobial activity was evaluated by microdilution assay in fluid medium. Among different extracts from roots and fruits, root ethyl acetate and fruit methanol extracts had higher total phenolic content, while fruit ethyl acetate extract contained higher total flavonoid content. The higher antioxidant activity was shown for roots ethanol extract (IC50=88 μg/ml). As its traditional uses, roots aqueous extract from C. spinosa exhibited inhibitory effect against bacteria and fungi with the exception of Pseudomonas aeruginosa. With the exception of fruit aqueous extract, other extracts (methanol, ethanol and ethyl acetate) from fruit and root exhibited good activity against microorganisms, especially fungi. This study reveals the traditional uses of roots and fruit extracts as antimicrobials agent.

scholarly journals A STUDY ON PHYTOCHEMICAL SCREENING, ANTIOXIDANT, ANTIMICROBIAL AND α-ΑMYLASE INHIBITORY ACTIVITIES OF CRUDE EXTRACTS OF THE STEM BARK OF PISONIA GRANDIS, R.BR

2017 ◽  
Vol 10 (11) ◽  
pp. 129
Author(s):  
Vishu Mohan ◽  
Mohan Kumar Ramasamy ◽  
Srikalyani Vemuri ◽  
Ilango Kaliappan
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Antidiabetic Activity ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid

  Objective: The aim of the present study is to determine the phytochemical screening, antioxidant activity and α-amylase inhibitory activity of the crude hexane, ethyl acetate and ethanolic stem bark extract of Pisonia grandis.Methods: The evaluation of antioxidant and antimicrobial activity, total phenolic, and flavonoid content were assessed using 2,2-diphenyl-1- picrylhydrazyl, Folin–Ciocalteu’s reagent, and aluminum chloride assay, respectively. The antidiabetic activity was assessed for porcine pancreatic α-amylase for the stem bark of P. grandis. Results: Phytochemical screening confirmed the presence of phenolic, flavonoids, tannins, saponins, terpenoids, and steroids in all the three extracts. The antioxidant activity showed 148.2 μg/ml, total phenolic content (gallic acid equivalent), 0.0665±0.0002 mg/g, flavonoid content (quercetin equivalent), 0.6061±0.1817 mg/g, and inhibitory concentration 50% values were found to be 40.42 μg/ml and showed better in ethyl acetate extract. The antidiabetic activity exhibited mimic action with insulin due to the presence of pinnatol in the stem bark and leaves of P. grandis. Conclusion: P. grandis stem bark crude ethyl acetate extract showed strong antioxidant activity, high phenolic, and flavonoid content. The antimicrobial activity was studied in both Gram-positive and Gram-negative strains against ampicillin and rifampicin as reference drugs. Antidiabetic activity shows effective result by α-amylase inhibitory activity.

scholarly journals Antimicrobial and antioxidant activity of Epimedium pinnatum

2013 ◽  
Vol 59 (2) ◽  
pp. 24-34 ◽  
Author(s):  
Mohaddese Mahboubi ◽  
Nastaran Kazempour ◽  
Hossein Hosseini ◽  
Mona Mahboubi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Methanol Extract ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Spectrophotometric Methods ◽  
Ic50 Value ◽  
Antioxidant And Antimicrobial Activity ◽  
Antimicrobial And Antioxidant Activity

Summary Epimedium pinnatum (Berberidaceae family) is used as an aphrodisiac in traditional Chinese medicine (TCM). The aim of this study was to evaluate the antimicrobial and antioxidant activity of E. pinnatum extracts (ethanol, methanol and aqueous extracts). Total phenolic (TPC) and flavonoid contents (TFC) of each extract were assessed by spectrophotometric methods. It was exhibited that methanol extract had better antimicrobial activity than those of ethanolic extract or aqueous extract. The TPC and TFC of E. pinnatum extracts was higher in methanol extract (149 and 36.6 mg/g) than that of ethanolic extract (137.2 and 19.5 mg/g) and aqueous extract (86.2 and 8.4 mg/g). The methanol extract had lower IC50 value (200 µg/ml) than ethanolic (250 µg/ml) and aqueous extract (400 µg/ml). There was a positive correlation between TPC, TFC in E. pinnatum extract and their antioxidant and antimicrobial activity

scholarly journals Profile of Phenolic and Antioxidants Activity from Seaweed Extract Turbinaria conoides and Eucheuma cottonii

2017 ◽  
Vol 20 (2) ◽  
pp. 230 ◽  
Author(s):  
Rini Yanuarti ◽  
N. Nurjanah ◽  
Effionora Anwar ◽  
Taufik Hidayat
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Phenolic Content ◽  
Polar Solvent ◽  
Seaweed Extract ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Antioxidants Activity ◽  
Turbinaria Conoides ◽  
Eucheuma Cottonii

Seaweed extract has ability to reduce some effects of free radicals because of phenolic compound. The purpose of this study was to determine total phenolic content, total flavonoids and antioxidant activity of seaweed extract from Turbinaria conoides and Eucheuma cottonii with different solvents. The extraction was carried out by n-hexane (non-polar) solvent, ethyl acetate (semi-polar) and methanol (polar). The results showed that the highest total phenol and flavonoids were found in  extract of Turbinaria conoides with ethyl acetate 211.00 mg GAE/g and 157.16 mg QE/g. Seaweed Eucheuma cottonii extract using methanol solvent had a higher phenol content 141.00 mg GAE/g, whereas the highest flavonoid content found in ethyl acetate solvent (35.1771 mg QE/g). The very strong antioxidant activity (< 50 μg/mL) was found in Turbinaria conoides and Eucheuma cottonii which extracted by methanol solvent (15.148 μg/mL and 23.154 μg/mL). Seaweed Turbinaria conoides and Eucheuma cottonii had a great potential as natural antioxidant.

scholarly journals Study on the Chemical Composition, Enzyme Inhibition and Antioxidant Activity of Ziziphora taurica subsp. cleonioides

2019 ◽  
Vol 9 (24) ◽  
pp. 5515 ◽  
Author(s):  
Cengiz Sarikurkcu ◽  
Eleni Kakouri ◽  
Rifat Tayyib Sarikurkcu ◽  
Petros A. Tarantilis
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Enzyme Inhibition ◽  
Methanolic Extract ◽  
Skin Pigmentation ◽  
Extraction Process ◽  
Total Phenolic ◽  
Chemical Profile ◽  
Flavonoid Content ◽  
And Oxidative Stress

Ziziphora is a plant used in Turkish and Iran traditional medicine for its antibacterial activity, sedative and stomach soothing properties. Although the chemical profile of the essential oil of different Ziziphora species is well documented, data regarding plant extracts are incomplete. In this study extracts from Ziziphora taurica subsp. cleonioides were obtained using ethyl acetate, methanol and water and the chemical profile of the aerial part of the plant was elucidated. Among the compounds identified, rosmarinic acid was the most abundant (3375.67 ± 38.02 μg/mL), at the extract of methanol, followed by chlorogenic acid (3225.10 ± 16.44). Enzyme inhibition activity against α-amylase and tyrosinase was also estimated. The ethyl acetate extract showed the highest α-amylase activity (1.95 ± 0.04 mg/mL), while the best anti-tyrosinase activity was calculated for the methanolic extract (1.25 ± 0.01 mg/mL). In addition, total phenolic, flavonoid content and antioxidant activity were evaluated. According to our results, bioactivity of the plant is of great interest, nonetheless, at the same time, it is strongly depended on the solvent used during the extraction process. Our data suggest that the plant under study may be an important source to consider against metabolic, skin pigmentation and oxidative stress related disorders.

Studies on In vitro Interaction of Ampicillin and Polyalthia longifolia Leaf Ethyl Acetate Fraction (PLEAF) by Checkerboard Method Against Methicillin Resistant Staphylococcus aureus (MRSA)

2020 ◽  
Vol 16 (7) ◽  
pp. 1049-1062
Author(s):  
Balasupramaniam Kirubakari ◽  
Yeng Chen ◽  
Jagat R. Kanwar ◽  
Lai N. Shin ◽  
Sreenivasan Sasidharan
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Cell Viability ◽  
Ethyl Acetate ◽  
Inhibitory Concentration ◽  
Ethyl Acetate Fraction ◽  
Vero Cells ◽  
Total Phenolic ◽  
Cytotoxicity Test ◽  
Polyalthia Longifolia

Background: Polyalthia longifolia which originates from India is rich with various useful phytochemicals which are valuable for human health. Accordingly, the current study was conducted to evaluate the combinational antimicrobial activity of P. longifolia Ethyl Acetate Fraction (PLEAF) with ampicillin, antioxidant and cytotoxicity activities. Methods: The evaluation of the synergistic activity of PLEAF fraction and ampicillin against MRSA local isolate was conducted with various antimicrobial assays. Results: The Minimum Inhibitory Concentration (MIC) values of PLEAF fraction (62.5 μg/mL) and ampicillin (5000 μg/mL) were found to decrease to 15.63 μg/mL for PLEAF and 2500 μg/mL for ampicillin respectively in the Fractional Inhibitory Concentration (FIC) assay against the MRSA bacteria. The 2,2-diphenyl1-picrylhydrazyl (DPPH) and nitric oxide free radical scavenging activities showed that PLEAF fraction possessed high antioxidant activity and the combinational of PLEAF fraction and ampicillin exhibited moderate antioxidant activity. The total phenolic content (TPC) of PLEAF was 168.22 ± 0.00407 μg GAE/g of PLEAF fraction. Discussion: henolic compounds might be responsible for the observed antioxidant and antimicrobial activity of PLEAF fraction. In addition, in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity test against Vero cells the PLEAF fraction was proven to be non-toxic (98.14% of cell viability) and the combination of PLEAF fraction and ampicillin treatment against the Vero cells showed an improved cell viability (52.44%) as compared with ampicillin alone in the treated group. Conclusions: The PLEAF fraction works well in combination with ampicillin to kill the MRSA local resistance strain. PLEAF fraction also showed favourable antioxidant activity and improved Vero cell viability in the presence of ampicillin which is an important attribute of PLEAF fraction to be used in the future combinational therapy.

A Comparative Study on Phytochemical Screening and Antioxidant Activity of Aqueous Extract from Various Parts of Moringa oleifera

2021 ◽  
Vol 3 (2) ◽  
pp. 43
Author(s):  
Awalul Fatiqin ◽  
Hanif Amrulloh ◽  
Ike Apriani ◽  
Aneke Lestari ◽  
Berta Erawanti ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Total Phenolic Content ◽  
Moringa Oleifera ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Leaf Part ◽  
Different Parts

In this work, we reported a comparative study on the phytochemical screening and antioxidant activity of aqueous extract from different parts of Moringa oleifera plant, i.e. leaves, twigs, stem barks, and woods. The phytochemical content of each extract was screened through qualitative analysis and followed by quantitative analysis for the determination of total phenolic content and total flavonoid content. The results showed that different parts of Moringa oleifera plant would give different extracted natural products. Either leaf or twig part of Moringa oleifera contained alkaloids, flavonoids, saponins, carbohydrates, polyphenols, proteins, amino acids, and phenolics. Meanwhile, only flavonoids, carbohydrates, and phenolics were found in the aqueous extract of the wood part of Moringa oleifera. In agreement to the phytochemical assay, the aqueous extract of leaf part of Moringa oleifera gave the highest total phenolic content (684 ± 16.7 µg GAE mL-1) and the highest flavonoid content (514 ± 26.1 µg CE mL-1) than other parts. Consequently, the leaf extract exhibited the highest antioxidant activity (64.6 ± 0.69%) which is close to the antioxidant activity of ascorbic acid (71.7 ± 0.56%) as the positive control. This finding is critical for the utilization of Moringa oleifera leaf part as the most potent source of antioxidant agents in the future.

scholarly journals ANTIOXIDANT ACTIVITIES AND TOTAL PHENOLIC AND FLAVONOID CONTENT VARIATIONS OF LEAF EXTRACTS OF LAURUS NOBILIS L. FROM MOROCCO

2018 ◽  
Vol 11 (12) ◽  
pp. 540 ◽  
Author(s):  
Amal Taroq ◽  
Fatima El Kamari ◽  
Imane Aouam ◽  
Yassine El Atki ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Laurus Nobilis ◽  
Dry Extract ◽  
Water Extracts ◽  
Phenolic And Flavonoid

Objective: The present study was undertaken to determine antioxidant activity and total phenolic and flavonoid content of ethanol, methanol, ethyl acetate, and aqueous extracts of the leaves of Laurus nobilis L.Methods: Antioxidant properties were measured using three tests: Free radical scavenging activity against 2,2-diphenyl picrylhydrazyl (DPPH), reduction of molybdate, and reducing (Fe3+/Fe2+) power. Total phenolic and flavonoid content was measured by Folin–Ciocalteu and rutin reagent, respectively.Results: The results showed that both the ethanol and water extracts had greater antioxidant activity than ethyl acetate. Aqueous extract exhibited a higher DPPH radical scavenging and reducing molybdate 545.83±5.89 mg equivalent to ascorbic acid/g dry extract. The strong antioxidant activity of water extract was probably due to its high content of phenols. Furthermore, ethanol and water extracts showed higher total flavonoid content with the values: 153.33±3.59 and 127.25±2.60 mg equivalent rutin/g dry extract, respectively.Conclusion: Our results suggested a potent and excellent antioxidant activity of L. nobilis L. extracts of Morocco. Further research has to be done to isolate the pure bioactive compound and could be considered as a potential source of biomolecules for pharmaceutical and food industry.

scholarly journals Fatty acid composition, phytochemicals and antioxidant potential of Capparis spinosa sedes

2022 ◽  
Vol 72 (4) ◽  
pp. e430
Author(s):  
A. Bodaghzadeh ◽  
K. Alirezalu ◽  
S. Amini ◽  
A. Alirezalu ◽  
R. Domínguez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Oil Content ◽  
Unsaturated Fatty Acids ◽  
Bioactive Molecules ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Capparis Spinosa

The present study evaluates the contents in bioactive compounds, antioxidant activity, oil content and fatty acid composition of Capparis spinosa seeds. Samples were collected from 5 different habitats (AH: Ahar; KU: Kurdistan; U1, U2 and U3: Urmia) in Iran. The oil content in the seeds ranged from 16 to 27%. The predominant fatty acid was linoleic acid (45-50%) followed by oleic acid (30-39%), palmitic acid (2-8%) and stearic acid (2-3%). Total phenolic content (TPC) varied from 16.3 to 24.2 mg GAE/ g DW; total flavonoid content (TFC) ranged from 1.48 to 3.05 mg QE/g DW; and the antioxidant activity (DPPH assay) of the seeds was between 35 and 63%. The compounds obtained from different genotypes of C. spinosa seeds had different compositions, great antioxidant capacity and unsaturated fatty acids, and therefore could be a prospective source of natural bioactive molecules for the food and health industry.

The influence of Gum Arabic on the physicochemical and antimicrobial activity of the microencapsulated Mahkota Dewa (Phaleria macrocarpa) leaves

Food Research ◽  
2021 ◽  
Vol 5 (3) ◽  
pp. 203-213
Author(s):  
Z. Mohd Zin ◽  
N.H. Razman ◽  
Hasmadi M. ◽  
M.N. Abd Manap ◽  
M.K. Zainol
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Gum Arabic ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Spherical Structure ◽  
Ultrasonic Spray ◽  
Phaleria Macrocarpa ◽  
Coating Matrix ◽  
Phenolic And Flavonoid

Mahkota Dewa (Phaleria macrocarpa) also known as God's Crown has been historically used as an indispensable alternative herbal medicine. Microencapsulation is a process whereby tiny particles or droplets are engulfed or enclosed in a coating matrix to produce small capsules. Generally, without microencapsulation, powders are fragile materials that could easily interfere with other components that are difficult to dissolve in water, lose their beneficial properties and decrease shelf life. It is hoped that the microencapsulation would increase the consistency of the powder during storage and maintain its beneficial properties. The goal of this research is to investigate the physicochemical and antimicrobial activity of Mahkota Dewa leaves encapsulated in different concentrations of gum Arabic (GA) and to determine the form of antioxidant and their role and properties. Mahkota Dewa leaves powders were microencapsulated in 0%, 2%, 4%, 6%, 8% and 10% gum Arabic using an ultrasonic spray dryer at 90οC. The microencapsulated Mahkota Dewa leaves (MMDL) samples were subjected to physicochemical and antimicrobial activity. The results showed that the 6% GA MMDL exhibited the highest yield (3.91%) while 0% GA was the lowest yield (1.64%). The highest total phenolic and flavonoid content was exhibited by 2% GA. The highest DPPH inhibition was depicted in 0% GA which indicates the highest antioxidant activity (54.9±0.01%) and is significantly (p<0.05) different from other samples. The highest inhibition was exhibited in 0% GA in the TBA method and FTC analysis. The encapsulated powders were identified to have weak antimicrobial activity against Bacillus cereus, Escherichia coli, Staphylococcus aureus, Salmonella and Listeria monocytogenes. The powders produced have an irregularly spherical structure and smooth surface with some dented spots on the surface. The different concentration of gum Arabic resulted in different antioxidant activity, flavonoid content and antimicrobial activity of MMDL.

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