Chronic exposure to cobalt compounds — an in vivo study

2014 ◽  
Vol 9 (10) ◽  
pp. 973-981
Author(s):  
Yordanka Gluhcheva ◽  
Vasil Atanasov ◽  
Juliana Ivanova ◽  
Ekaterina Pavlova
Keyword(s):  
Body Weight ◽  
Cobalt Chloride ◽  
Tap Water ◽  
Organ Weight ◽  
Duration Of Treatment ◽  
Cobalt Ions ◽  
Cobalt Compounds ◽  
Pregnant Mice

AbstractAn in vivo experimental model for testing the effects of long-term chronic treatment with cobalt(II) compounds — cobalt chloride (CoCl2) and cobalt-EDTA (Co-EDTA) on mice at different stages of development was optimized. Pregnant mice and their progeny were treated with daily doses of 75 or 125 mg kg−1 body weight until postnatal day 90. The compounds were dissolved in regular tap water. Mice were sacrificed on days 18, 25, 30, 45, 60 and 90 after birth, which correspond to different stages of their development. Altered organ weight indices (calculated as a ratio of organ weight to body weight) of spleen, liver and kidneys, were found depending on the type of compound used, dose, duration of treatment, and the age of the animals. The results also showed significant accumulation of cobalt ions in blood plasma, spleen, liver and kidneys of the exposed mice. More Co(II) was measured in the organs of the immature mice (day 18, 25 and 30 pnd) indicating that they were more sensitive to treatment.

scholarly journals Toxicological evaluation of aqueous extract of the traditional Chinese formula Qing Hao Gan Cao

2021 ◽  
Author(s):  
Yongchun Li ◽  
Hui Zhang ◽  
Shanshan Chen ◽  
Liutao Zhao ◽  
Jie Wu ◽  
...  
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Toxicity Test ◽  
Artemisia Annua ◽  
Hematological Parameters ◽  
Organ Weight ◽  
Toxicological Evaluation ◽  
Subacute Toxicity ◽  
Qing Hao

Abstract Qing Hao Gan Cao (QHGC), a Chinese medicinal formula containing Artemisia annua and Glycyrrhizae Radix et Rhizoma, has been used to treat sunstroke and as an antiviral agent for more than 800 years. It has not previously been subject to a toxicological safety evaluation in acute and subacute (28 days) studies. Therefore, the acute and subacute toxicity of an aqueous extract of QHGC were evaluated in vivo. For the QHGC preparation, the botanical raw materials were crushed into pieces and mixed in the ratio of 10:1 in distilled water for 12 h, then boiling three times for 2 h each time. The three decoctions were mixed and filtered, then spray-dried with hot air at 160°C for 30 min, and stored at room temperature. For the acute toxicity test, 72.0 g/kg of QHGC extract was administered by gavage to male and female mice. Body weight, general observations, and autopsy results were recorded. No mortality or toxicity signs were observed during the studies. For the subacute toxicity test, 4.0, 8.0, or 16.0 g/kg/day of QHGC extract was administered to rats for 28 days. General observations and mortality, body weight, biochemical and hematological parameters, organ weight, and pathological morphology were analyzed. The acute and subacute toxicity studies did not show significant changes in body weight, general observations, hematology and biochemical parameters, organ weight, and liver, spleen, stomach, duodenum, testis, ovary, lung, heart, and kidney histopathological analyses. The consumption of QHGC aqueous extract can be considered safe within the conditions of this study.

Abstract 228: Alterations of Cardiac Morphology and Function Caused by Cardio-Thoracic Surgery in Small Animal Models of Heart Disease

2013 ◽  
Vol 113 (suppl_1) ◽  
Author(s):  
Peter Nordbeck ◽  
Leoni Bönhof ◽  
Karl-Heinz Hiller ◽  
Sabine Voll ◽  
Paula Arias ◽  
...  
Keyword(s):  
Body Weight ◽  
Heart Disease ◽  
Animal Models ◽  
Right Ventricular ◽  
Small Animal ◽  
Cardiac Morphology ◽  
Small Animal Models ◽  
And Function

Background: Surgical procedures in small animal models of heart disease, such as artificial ligation of the coronary arteries for experimental myocardial infarction, can evoke alterations in cardiac morphology and function. Such alterations might induce artificial early or long term effects in vivo that might account for a significant bias in basic cardiovascular research, and, therefore, could potentially question the meaning of respective studies in small animal models of heart disease. Methods: Female Wistar rats were matched for weight and distributed to sham left coronary artery ligation or untreated control. Cardiac parameters were then investigated in vivo by high-field MRI over time after the surgical procedure, determining left and right ventricular morphology and function. Additionally, the time course of several metabolic and inflammatory blood parameters was determined. Results: Rats after sham surgery showed a lower body weight for up to 8 weeks after the intervention compared to healthy controls. Left and right ventricular morphology and function were not different in absolute measures in both groups 1 week after surgery. However, there was a confined difference in several cardiac parameters normalized to the body weight (bw), such as myocardial mass (2.19±0.30/0.83±0.13 vs. 1.85±0.22/0.70±0.07 mg left/right per g bw, p<0.05), or enddiastolic ventricular volume (1.31±0.36/1.21±0.31 vs. 1.14±0.20/1.07±0.17 µl left/right per g bw, p<0.05). Vice versa, after 8 weeks, cardiac masses, volumes, and output showed a trend for lower values in the sham operated rats compared to the controls in absolute measures (782.2±57.2/260.2±33.2 vs. 805.9±84.8/310.4±48.5 mg, p<0.05 for left/right ventricular mass), but not normalized to body weight. Matching these findings, blood testing revealed prolonged metabolic and inflammatory changes after surgery not related to cardiac disease. Conclusion: There is a small distinct impact of cardio-thoracic surgical procedures on the global integrity of the organism, which in the long term also includes circumscribed repercussions on cardiac morphology and function. This impact has to be considered when analyzing data from respective studies and transferring the findings to conditions in patients.

Long-term passage of tachyzoites in tissue culture can attenuate virulence of Neospora caninum in vivo

Parasitology ◽  
2006 ◽  
Vol 133 (4) ◽  
pp. 421-432 ◽  
Author(s):  
P. M. BARTLEY ◽  
S. WRIGHT ◽  
J. SALES ◽  
F. CHIANINI ◽  
D. BUXTON ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Body Weight ◽  
Growth Rates ◽  
Clinical Symptoms ◽  
Neospora Caninum ◽  
High Passage ◽  
Low Passage

To determine whether prolonged in vitro passage would result in attenuation of virulence in vivo, Neospora caninum tachyzoites were passaged for different lengths of time in vitro and compared for their ability to cause disease in mice. Groups of Balb/c mice were inoculated intraperitoneally with 5×106 or 1×107 of low-passage or high-passage N. caninum tachyzoites. The mice were monitored for changes in their demeanour and body weight, and were culled when severe clinical symptoms of murine neosporosis were observed. Mice inoculated with the high-passage parasites survived longer (P<0·05), and showed fewer clinical symptoms of murine neosporosis, compared to the mice receiving the low-passage parasites. The parasite was detected in the brains of inoculated mice using immunohistochemistry and ITS1 PCR. Tissue cysts containing parasites were seen in mice inoculated with both low-passage and high-passage parasites. When the in vitro growth rates of the parasites were compared, the high-passage parasites initially multiplied more rapidly (P<0·001) than the low-passage parasites, suggesting that the high-passage parasites had become more adapted to tissue culture. These results would suggest that it is possible to attenuate the virulence of N. caninum tachyzoites in mice through prolonged in vitro passage.

Schistosoma mansoni: structural damage and tegumental repair after in vivo treatment with praziquantel

Parasitology ◽  
1987 ◽  
Vol 94 (2) ◽  
pp. 243-254 ◽  
Author(s):  
M. K. Shaw ◽  
D. A. Erasmus
Keyword(s):  
Immune Response ◽  
Body Weight ◽  
Schistosoma Mansoni ◽  
Structural Damage ◽  
Post Treatment ◽  
Drug Induced ◽  
Male And Female ◽  
Extensive Damage

SUMMARYThe long-term, in vivo effects of a single, subcurative dose (200 mg/kg body weight of mouse) of praziquantel on the structure of adult Schistosoma mansoni and on the process and speed of tegumental repair are described. In both male and female worms praziquantel caused often extensive damage to the tegument, in the form of surface blebbings, swellings and lesions, and vacuolization and disruption of the subtegumental tissues. Repair of the drug-induced tegumental damage occurred slowly with partial and, more rarely, complete repair only being seen after 65 days post-treatment (p.t.), although signs of damage were still observed, particularly in male worms, at 100 days p.t. In contrast, repair of damage to the subtegumental/parenchymal tissues including the tegumental perikarya occurred relatively quickly, with the majority of worms examined appearing normal by 8–12 days p.t. The possible role(s) of the host immune response in relation to the speed of tegumental repair in vivo is discussed.

The influence of long-term melatonin administration on basic physiological and metabolic variables of young Wistar:Han rats

Biologia ◽  
2006 ◽  
Vol 61 (3) ◽  
Author(s):  
Monika Kassayová ◽  
Martina Marková ◽  
Bianka Bojková ◽  
Eva Adámeková ◽  
Peter Kubatka ◽  
...  
Keyword(s):  
Body Weight ◽  
Water Intake ◽  
Glucose Concentration ◽  
Tap Water ◽  
Serum Glucose ◽  
The Body ◽  
Male Rats ◽  
Epididymal Fat ◽  
Food And Water Intake

AbstractThe question of effects of long-term melatonin (MEL) administration have not yet been explained sufficiently, especially its metabolic consequences in young persons and animals. The aim of the present study was to analyze the effects of MEL given during prolonged time (for 3 months) and chronically (for 6 months) at the dose of 4 µg/mL of tap water, on the selected metabolic and hormonal parameters in young female and male Wistar:Han (WH) rats. The weights of selected organs, tissues, body weight gains and food and water intake were registered. Six weeks aged rats were adapted to standard housing conditions and light regimen L:D=12:12 h, fed standard laboratory diet and drank tap water (controls) or MEL solution ad libitum; finally they were sacrificed after overnight fasting. Prolonged MEL administration decreased serum glucose concentration and increased triacylglycerol and malondialdehyde concentration/content in the liver in females. In males MEL increased concentrations of serum phospholipids, corticosterone and liver malondialdehyde. MEL treatment reduced the body weight in both sexes and weight of epididymal fat in males, without any alterations of food and water intake. Chronic MEL administration reduced serum glucose concentration and increased concentration/content of glycogen, triacylglycerol and cholesterol in the liver and glycogen concentration/content in heart muscle in males. In females, the significant rise of serum corticosterone concentration and liver malondialdehyde content was recorded. MEL significantly increased liver weight and decreased thymus weight in males. MEL administration increased temporarily water intake in males, body and epididymal fat weights were similar to that in controls. Body weight of MEL drinking females was reduced in the 1st half of experiment only; the food and water intake did not differ from control group. The response in WH rats on MEL was more prominent as in the Sprague-Dawley strain (our previous studies). Male rats were generally more affected, probably due to higher daily and total consumption of melatonin.

scholarly journals Effects of long-term in vivo micro-CT imaging on hallmarks of osteopenia and frailty in aging mice

2020 ◽  
Author(s):  
Ariane C. Scheuren ◽  
Gisela A. Kuhn ◽  
Ralph Müller
Keyword(s):  
Body Weight ◽  
Aging Process ◽  
Frailty Index ◽  
Ct Imaging ◽  
Morphometric Parameters ◽  
The Body ◽  
The Other ◽  
Micro Ct

AbstractIn vivo micro-CT has already been used to monitor microstructural changes of bone in mice of different ages and in models of age-related diseases such as osteoporosis. However, as aging is accompanied by frailty and subsequent increased sensitivity to external stimuli such as handling and anesthesia, the extent to which longitudinal imaging can be applied in aging studies remains unclear. Consequently, the potential of monitoring individual mice during the entire aging process – from healthy to frail status – has not yet been exploited. In this study, we assessed the effects of long-term in vivo micro-CT imaging - consisting of 11 imaging sessions over 20 weeks - on hallmarks of aging both on a local (i.e., static and dynamic bone morphometry) and systemic (i.e., frailty index (FI) and body weight) level at various stages of the aging process. Furthermore, using a premature aging model (PolgA(D257A/D257A)), we assessed whether these effects differ between genotypes.The 6th caudal vertebrae of 4 groups of mice (PolgA(D257A/D257A) and PolgA(+/+)) were monitored by in vivo micro-CT every 2 weeks. One group was subjected to 11 scans between weeks 20 and 40 of age, whereas the other groups were subjected to 5 scans between weeks 26-34, 32-40 and 40-46, respectively. The long-term monitoring approach showed small but significant changes in the static bone morphometric parameters compared to the other groups. However, no interaction effect between groups and genotype was found, suggesting that PolgA mutation does not render bone more or less susceptible to long-term micro-CT imaging. The differences between groups observed in the static morphometric parameters were less pronounced in the dynamic morphometric parameters. Moreover, the body weight and FI were not affected by more frequent imaging sessions. Finally, we observed that longitudinal designs including baseline measurements at young adult age are more powerful at detecting effects of in vivo micro-CT imaging on hallmarks of aging than cross-sectional comparisons between multiple groups of aged mice subjected to fewer imaging sessions.

scholarly journals Effects of Cimetidine on Broiler Fattening and on Stress-Induced Gizzard Erosion in Chicken

1999 ◽  
Vol 47 (2) ◽  
pp. 233-241 ◽  
Author(s):  
Ž. Grabarević ◽  
P. Džaja ◽  
J. Perić ◽  
V. Šerman ◽  
Z. Biđin ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Morphometric Analysis ◽  
Tap Water ◽  
Water Immersion ◽  
Carcass Weight ◽  
Feed Consumption ◽  
Control Group ◽  
Feed Deprivation

The work describes the effects of cimetidine on stress-induced gizzard erosions (Experiment A) and the influence of the long-term application (42 days) of the same drug on weight gain and feed consumption during broiler fattening (Experiment B). For Experiment A, 60 male, three-day-old chicks were divided into two groups: C (n = 30) - control chicks treated with 0.5 ml saline; CIM (n = 30) - chicks treated with cimetidine in a dose of 5 mg/kg body weight (b. w.) in-tragastrically. All chicks were stressed using a modified water-immersion stress method according to which the chicks, after 24 h of feed deprivation, were immersed in tap water (17 °C) for a few seconds. Under chloroform anaesthesia ten chicks from each group were killed 1, 2 and 3 h after the stressing. The morphometric analysis of gizzard erosion (GE) and histopathological examinations of gizzards were performed for each chick. In Experiment B, 32 one-day-old broilers of both sexes were used. The control group was untreated (n = 16) while the CIM group (n = 16) was fed the same diet supplemented with 10 mg of cimetidine per kilogram of feed throughout the fattening period (42 days). The results of Experiment A showed decreased mean length of the GE in the cimetidine-treated birds as compared with the GE lesions of the controls. In Experiment B, the treated chicks had reduced liveweight (1835.1 g), carcass weight (1474.6 g) and increased feed consumption (2115 g of feed per kilogram of weight gain) compared to the controls in which the same parameters were 1898.5 g, 1574.2 g and 1797 g, respectively. The results show that while stress-induced GE of chicks can be medicated pharmacologically, long-term application of the same substance impairs the results of fattening.

scholarly journals Different Effects of Three Types of Water on Developmental Behaviors, Lipid Metabolism and Antioxidant Capacity of Juvenile Zebrafish

2018 ◽  
Author(s):  
Cheng Chang ◽  
Jiajin Zhu
Keyword(s):  
Body Weight ◽  
Lipid Metabolism ◽  
Antioxidant Capacity ◽  
Natural Water ◽  
Growth And Development ◽  
Tap Water ◽  
Adequate Nutrition ◽  
Drinking Waters ◽  
Healthy Growth

AbstractAdolescence is an important period when people need adequate nutrition for healthy growth and development, whether from food or water. Tap water, bottled water (bottled purified water, and bottled natural water) are now the three most popular drinking waters consumed by adolescents in China. However, the constituents of them differ, which may cause different long-term health effects. In order to determine which type of water is the most beneficial regarding developmental behaviors, lipid metabolism and antioxidant capacity of juvenile zebrafish, 21 dpf (days post-fertilization) zebrafish were given these three waters separately with the same feed until 90 dpf. Results showed that zebrafish in purified water had the lowest survival rate, body weight and body length, while zebrafish in natural water and tap water had similar developmental behaviors; the highest HDL-C level and the lowest TG level were found in natural water, tap water second; and zebrafish in natural water showed the best antioxidant capacity. Thus, the best outcomes were found in natural water, which had suitable pH and proper amount of minerals, tap water also showed good performance, while purified water seemed not suitable for juvenile zebrafish.

scholarly journals Impacts of Prepubertal Rearing Intensity and Calf Health on First-Lactation Yield and Lifetime Performance

2019 ◽  
Vol 19 (1) ◽  
pp. 201-214
Author(s):  
Nina Volkmann ◽  
Nicole Kemper ◽  
Anke Römer
Keyword(s):  
Body Weight ◽  
Milk Yield ◽  
Average Daily Gain ◽  
Long Term Effects ◽  
Duration Of Treatment ◽  
Feeding Intensity ◽  
Long Term Study ◽  
Lifetime Performance ◽  
Daily Gain

AbstractThe objective of the present study was to investigate possible long-term effects of calf and heifer feeding intensity on first-lactation milk yield and lifetime efficiency (milk per day of life). Detailed records from a total number of 2,252 female German Holstein calves from one commercial farm were obtained from birth to culling. Data regarding all information about calf’s birth, rearing time until first insemination, first calving, first-lactation yield and lifetime performance were collected over a 12-year period. This large data volume was merged, handled, checked for plausibility, classified and evaluated. Analyses revealed that body weight at an age of six months was significantly influenced by the average daily gain in the first two weeks of life (P<0.0001) and by the duration of treatment for respiratory diseases (P=0.0080). Moreover, first-lactation yield was affected by average daily gain in the whole first year of life (P=0.0013) and particularly in the period of month nine to twelve (P=0.00187). Lifetime efficiency was significantly influenced by body weight at first insemination (P=0.0051), average milk yield (P<0.0001) and reason for culling (P<0.0001). The results of this long-term study confirm that growth is important in general, but as shown by the negative correlation between average daily gain from month nine to twelve and the first lactation milk yield, nutrient intake (energy and protein) should be adapted to enable a controlled growth especially at certain periods of life. After six months of age, daily gain has to be controlled to avoid fat accumulation for a healthy and effective start of lactation. In addition, this analysis revealed that after passing first lactation, particularly health and fertility are the keys for a long efficient lifetime of dairy cows.

scholarly journals Genome sequencing and transcriptome analyses of the Siberian hamster hypothalamus identify mechanisms for seasonal energy balance

2019 ◽  
Vol 116 (26) ◽  
pp. 13116-13121 ◽  
Author(s):  
Riyue Bao ◽  
Kenneth G. Onishi ◽  
Elisabetta Tolla ◽  
Fran J. P. Ebling ◽  
Jo E. Lewis ◽  
...  
Keyword(s):  
Body Weight ◽  
Energy Balance ◽  
Food Availability ◽  
Molecular Mechanisms ◽  
Thyroid Hormone Receptor ◽  
Binding Motif ◽  
Siberian Hamster ◽  
Species Specific

Synthesis of triiodothyronine (T3) in the hypothalamus induces marked seasonal neuromorphology changes across taxa. How species-specific responses to T3signaling in the CNS drive annual changes in body weight and energy balance remains uncharacterized. These experiments sequenced and annotated the Siberian hamster (Phodopus sungorus) genome, a model organism for seasonal physiology research, to facilitate the dissection of T3-dependent molecular mechanisms that govern predictable, robust, and long-term changes in body weight. Examination of thePhodopusgenome, in combination with transcriptome sequencing of the hamster diencephalon under winter and summer conditions, and in vivo-targeted expression analyses confirmed that proopiomelanocortin (pomc) is a primary genomic target for the long-term T3-dependent regulation of body weight. Further in silico analyses ofpomcpromoter sequences revealed that thyroid hormone receptor 1β-binding motif insertions have evolved in several genera of the Cricetidae family of rodents. Finally, experimental manipulation of food availability confirmed that hypothalamicpomcmRNA expression is dependent on longer-term photoperiod cues and is unresponsive to acute, short-term food availability. These observations suggest that species-specific responses to hypothalamic T3, driven in part by the receptor-binding motif insertions in some cricetid genomes, contribute critically to the long-term regulation of energy balance and the underlying physiological and behavioral adaptations associated with the seasonal organization of behavior.

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