Clinico-immunological justification of the use of a new dental ointment developed on the basis of a long-acting beekeeping product for topical treatment of inflammatory parodontal diseases

2019 ◽  
Vol 24 (1) ◽  
pp. 94-100
Author(s):  
A. I. Bulgakova ◽  
N. A. Vasilieva ◽  
Y. V. Shikova ◽  
E. A. Imelbayeva ◽  
F. R. Ahmadeeva
Keyword(s):  
Periodontal Diseases ◽  
Control Level ◽  
Clinical State ◽  
Base Line ◽  
Local Immunity ◽  
Clinical Indicators ◽  
Periodontal Tissues ◽  
Group I ◽  
Long Acting ◽  
Mixed Saliva

Relevance of the research topic. The composition and technology for the manufacture of dental ointment based on antimicrobial metronidazole, anesthetic anesthesin and an immunomodula-tor are substantiated and developed - for the frst time a biological product of the beekeeping product of a large wax moth larvae extract.Puprose – the goal is a clinical and immunological rationale for the use of dental ointment developed composition based on the product of beekeeping for the treatment of inflammatory periodontal diseases. Materials and methods. Studies have been conducted in patients with inflammatory periodontal disease (CDL), aged from 20 to 70 years. Group I control (n = 25), who had no dental pathology and practically healthy at the time of examination, group II (n = 204) received basic thera-py, group III (n = 120) used a developed dental ointment based on ( Galleria mellonela). Evaluation of the effectiveness of treatment was carried out according to clinical and laboratory parameters. Analysis of the hygienic and clinical state of periodontal tissues was taken into account by the Green-Vermillion, Russel, PMA indices in the Parma modifcation. The state of local immunity was investi-gated according to the indicators of the leukocyte migration test, the quantitative and qualitative con-tent of spontaneously released mixed saliva, the content of total protein in it, secretory immunoglobu-lin A, lysozyme.Results. The effectiveness of the developed dental ointment is confrmed by the positive dy-namics of clinical indicators in comparison with the basic therapy in 72.3% of cases. The number of sessions per course of treatment for gingivitis and mild periodontitis was reduced to 3, moderate –5–6, and severe to 8 sessions. Indices (GI, PI, PMA) decreased by 6.7; 2.6; 5.1 times, respectively, in comparison with the group before treatment and in 3; 2; 2.6 times, respectively, compared with base-line therapy. Local immunity revealed: an increase in the production of mixed saliva in comparison with baseline data by 2.6 times, its recovery and an increase of 1 ml above the control level (p> 0.05), an increase in protein level (p <0.05), lysozyme concentration and sIgA exceeded the level before the treatment and after the baseline therapy, however, the indices did not recover to the level in the con-trol.Summaru. Inclusion of dental ointment on the basis of Galleria mellonell into the complex therapy of VZP is a means of correcting violations of local immunity and is a promising new dosage form in practical periodontology

scholarly journals IMMUNOLOGICAL AND PATHOPHYSIOLOGICAL LAWS OF LOCAL IMMUNITY ORAL CAVITY PREVENTION OF INFLAMMATORY COMPLICATIONS OF TREATMENT OF DENTOALVEOLAR ANOMALIES

2018 ◽  
Vol 22 (1) ◽  
pp. 25-28
Author(s):  
Pavel Aleksandrovich Zheleznyi ◽  
K. S Shchelkunov ◽  
S. P Zheleznyi ◽  
A. P Zheleznaia ◽  
Y. N Belousov
Keyword(s):  
Orthodontic Treatment ◽  
Antimicrobial Agents ◽  
Immunoglobulin A ◽  
Interleukin 4 ◽  
Activity Levels ◽  
Local Immunity ◽  
Immunomodulatory Agents ◽  
Periodontal Tissues ◽  
Mixed Saliva ◽  
Secretory Immunoglobulin

The state of local immunity oral factors in orthodontic treatment with fixed structures in 192 people aged 18-25 years. The mixed saliva was determined lysozyme activity levels of secretory immunoglobulin A, interleukin 1в and interleukin 4 dynamics. It was found that orthodontic treatment leads to activation of the inflammatory process in periodontal tissues, effective therapy which should be based on the integrated use of antimicrobial agents and immunomodulatory agents.

scholarly journals EVALUATION OF LOCAL IMMUNITY OF MORBIDITY OF THE MUTUAL IN THE TRADITIONAL THERAPY OF INFLAMMATORY DISEASES OF THE PARODONT

2018 ◽  
Vol 14 (3) ◽  
pp. 11-16 ◽  
Author(s):  
Надежда Васильева ◽  
Nadezhda Vasil'eva ◽  
Альбина Булгакова ◽  
Al'bina Bulgakova ◽  
Эльмира Имельбаева ◽  
...  
Keyword(s):  
Inflammatory Diseases ◽  
Periodontal Diseases ◽  
Effective Means ◽  
Immunoglobulin A ◽  
Control Group ◽  
Total Protein Content ◽  
Total Production ◽  
Local Immunity ◽  
Traditional Therapy ◽  
Mixed Saliva

Subject. The state of local immunity before and with the use of traditional therapy is considered. As a material for assessing local immunity, the quantitative and qualitative characteristics of mixed saliva and the migration test of leukocytes were used, reflecting the severity of inflammatory phenomena in the periodontium and to determine the effectiveness of treatment. The purpose is an evaluation of local immunity of the oral cavity in the traditional therapy of inflammatory periodontal diseases. Methodology. Local immunity was studied in 204 patients with periodontal inflammatory diseases according to the test of leukocyte migration, quantitative and qualitative content of spontaneously secreted mixed saliva: the total protein content, secretory immunoglobulin A (sIgA), lysozyme. All patients underwent traditional therapy. Results. The study revealed an imbalance of local immunity and was characterized by a decrease in total production of mixed saliva in all groups of CDW patients in comparison with the control group. The level of protein was reduced in the group of moderate hippocampus, sIgA in mixed saliva in all patients, especially in cases of severe HCV. The amount of lysozyme is increased in patients with gingivitis, in mild to moderate HGP it corresponds to the content of the control group, it is decreased in patients with moderate and severe periodontitis. In the course of traditional treatment, all patients showed a tendency to normalization of these parameters and was characterized by preservation of reduced production of mixed saliva in all CDW patients, increased lysozyme content and sIgA. However, the restoration of sIgA level in CDW patients to the control group level was not observed. In the group of severe hCG, the level of lysozyme was lower than in control. Conclusions. The use of traditional therapy indicates the preservation of the intensity of immune response in patients with CDW and requires further research and more effective means of action on inflammatory mediators.

Physiological substantiation of drug-free methods of patients with chronic catarrhal gingivitis rehabilitation

2019 ◽  
pp. 53-62
Author(s):  
Elena V. NEVZOROVA ◽  
Andrey A. FLENKIN ◽  
Sabir E. OSMANOV
Keyword(s):  
Olive Oil ◽  
Clinical Efficacy ◽  
Periodontal Diseases ◽  
The State ◽  
Theoretical Justification ◽  
Local Immunity ◽  
Clinical Indicators ◽  
Modern Times ◽  
Medical Ozone ◽  
Drug Free

Treatment of inflammatory periodontal diseases is one of the most urgent problems in dental practice. If we take into account the increase in the level of population allergization, tolerance of microorganisms and their associations to drugs, the approach to treatment with drug-free methods remains very relevant. In this study we deals with the etiological factors of chronic catarrhal gingivitis, we analyze the main means of rehabilitation in this disease, which are used in modern times, justified drug-free approach in the rehabilitation method. As the purpose of the study, we identify the theoretical justification and development of drug-free rehabilitation techniques for chronic catarrhal gingivitis, based on the local application of therapeutic concentrations of medical ozone. In this study we investigate the clinical efficacy of topical application of ozonized olive oil in the complex treatment of patients with chronic catarrhal gingivitis. During the experiment, the high clinical efficacy of the ozonated olive oil method in patients with chronic catarrhal gingivitis was observed, which was used to optimize the pro/antioxidant body system, improve the state of the autonomous system of local immunity of the oral cavity, significantly reduce the values of hygienic indices characterizing the state of the periodontium, significantly reduce the content of microorganisms and their associations, improve clinical indicators. The application method of ozonated olive oil in patients with chronic catarrhal gingivitis, has a therapeutic and healing effect, and also meets the requirements for technologies of periodontal rehabilitation.

The effectiveness of a dental gel with barberry extract in the treatment of plaque-induced gingivitis in orthodontic patients undergoing treatment with fixed appliances

2021 ◽  
Vol 21 (1) ◽  
pp. 51-56
Author(s):  
A. G. Skubitskaya ◽  
I. V. Firsova ◽  
S. V. Poroyskyi ◽  
O. G. Strusovskaya
Keyword(s):  
Orthodontic Treatment ◽  
Periodontal Diseases ◽  
Orthodontic Appliances ◽  
Local Immunity ◽  
Group I ◽  
Fixed Orthodontic Appliances ◽  
Tnf Α ◽  
Orthodontic Patients ◽  
Fixed Appliances ◽  
Group Ii

Relevance. The high necessity in orthodontic treatment among young patients and the use of fixed orthodontic appliances for the treatment of dental pathology are the factors that initiate inflammatory periodontal processes. Timely diagnosis and optimal preventive and treatment procedures can prevent or stop the inflammatory periodontal complications which could have developed during the orthodontic treatment. One of the effective methods for the prevention and treatment of inflammatory periodontal diseases is the use of targeted antimicrobial agents based on medicinal plants that have specific antimicrobial activity against periodontal pathogens. Purpose – to study the effectiveness of the dental gel with barberry extract for the treatment of plaque-induced gingivitis in orthodontic patients during the active phase of the treatment with fixed appliances.Materials and methods. The study included 60 patients (35 women and 25 men) aged 18 to 35 years who had presented for the orthodontic care. The patients undergoing orthodontic treatment with fixed appliances were randomly divided into two groups. In group I, the patients were prescribed applications of a dental gel with barberry extract; in group II, this medication was not prescribed. The clinical condition of the periodontium was assessed with periodontal indices PMA, SBI and OHI-S. The immune status was evaluated by the level of secretory IgA, interleukins (IL-1β and IL-4) and tumor necrosis factor-α (TNF-α). The patients were followed up during 1 month of treatment. The results were statistically processed with Statsoft Statistica 8.0 and Graph Pad Prism 5.0.Results. In group II, the production of immunoglobulin IgA, pro- and anti-inflammatory cytokines was impaired after a month of treatment, associated with a significant deterioration in the oral hygiene status and an increase in PMA (by 3 times), SBI (by 5.3 times) indices. In group II, the oral fluid concentrations of IL-1β, IL-4 and TNF-α significantly differed from those in group I as of the 7th day of the observation period and indicated suppression of the mucosal immunity. Clinical and immune parameters of the group I patients improved and were comparable with the control by the end of the observation month.Conclusions. Patients with dental abnormalities are at risk of developing inflammatory periodontal diseases. The results of the immunological tests, performed during the treatment with the use of a dental gel with barberry extract, demonstrated the recovery of the local immunity, as evidenced by the elevation of secretory immunoglobulin A in saliva and improvement of cytokine profile parameters.

scholarly journals Characteristics of Periodontal Tissues in Prosthetic Treatment with Fixed Dental Prostheses

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1331
Author(s):  
Anna Avetisyan ◽  
Marina Markaryan ◽  
Dinesh Rokaya ◽  
Marcos Roberto Tovani-Palone ◽  
Muhammad Sohail Zafar ◽  
...  
Keyword(s):  
Periodontal Diseases ◽  
Group Iii ◽  
Periodontal Tissues ◽  
Dental Prostheses ◽  
Group I ◽  
Periodontal Index ◽  
Computer Aided ◽  
Cad Cam ◽  
Group Ii ◽  
Gingival Biotype

The objective of the present study was to investigate the effects of various types of fixed prostheses on periodontal tissues and explore the association of gingival biotype and gum recession in relation to prosthesis types. The study participants (N = 95) were divided into three groups based on the type of dental prosthesis: Group-I: cobalt-chrome (Co-Cr) ceramic prosthesis fabricated by the conventional method (n = 35); Group-II: consisted of patients with Co-Cr ceramic prostheses fabricated by a computer-aided design and computer aided manufacturing (CAD/CAM) technique (n = 30); and Group-III: zirconia-based prostheses fabricated by the CAD/CAM technique (n = 30). Following the use of prostheses, periodontal examinations were performed using the Community Periodontal Index (CPI) and Modified Approximal Plaque Index (MAPI). In addition, the gingival biotype was examined using a probe transparency method. The Statistical Package for the Social Sciences (SPSS), Version 20 (IBM Company, Chicago, IL, USA), was used to analyze the results, and the significance level was set at p = 0.05. It showed the MAPI results after the use of prosthetic rehabilitation for 12 months of periodontitis in 87.9% ± 15.4 of patients in Group-I, in 80.6% ± 17.97 in those in Group-II, and in 62.5% ± 21.4 in those in Group-III (p < 0.01). The CPI index results indicated a high prevalence of periodontal disease in all groups. The number of people with healthy periodontium constituted 17.1% of patients in Group-I, 24.2% in Group-II, and 37.1% in Group-III. Our study concluded that prosthetic treatment with periodontal diseases showed better outcomes while using dental prostheses fabricated by the CAD/CAM technique compared to the conventionally fabricated dental prostheses. The thin gingival biotype is more often associated with gingival recession than the thick biotype.

EVALUATION OF THE EFFECTIVENESS OF COMPLEX TREATMENT AND COMPLIANCE OF PATIENTS WITH PERIODONTAL TISSUE DISEASES

2020 ◽  
pp. 42-55
Author(s):  
Наталия Владимировна Чиркова ◽  
Алена Алексеевна Плутахина ◽  
Алик Эдикович Петросян ◽  
Елена Анатольевна Андреева ◽  
Марина Николаевна Бобешко
Keyword(s):  
Oral Cavity ◽  
Antibiotic Therapy ◽  
Dental Treatment ◽  
Periodontal Diseases ◽  
Medical Science ◽  
Young Patients ◽  
Young Age ◽  
Nonspecific Resistance ◽  
Local Immunity ◽  
Periodontal Tissues

Лечение заболеваний пародонта представляет собой одну из наиболее сложных и важных проблем современной стоматологии, актуальность которой обусловлена высокой частотой воспалительных заболеваний пародонта среди населения во всех возрастных группах - до 90% в популяции. Несмотря на прогрессивное развитие медицинской науки и практики, заболеваемость данной патологией сохраняется на высоком уровне не только в группах населения среднего и пожилого возраста, но при этом выявляется тенденция к ее распространению в молодом возрасте. Исследования, проведенные ВОЗ более, чем в 30 странах, выявили высокую распространенность заболеваний пародонта (свыше 60%) среди пациентов возрасте от 18 до 30 лет. Наиболее частой формой поражения околозубных тканей в молодом возрасте является хронический гингивит по классификации СКБ-10 К 05.1. Нерегулярность профилактики и эпизодические обращения к врачу-пародонтологу для лечения заболеваний пародонтита утяжеляют течение заболевания, которое при отсутствии лечения прогрессирует. В связи с этим, от пациента требуется неоднократное прохождение курсов лечения у врача-пародонтолога и выполнение в домашних условиях профилактических мероприятий по рекомендации врача. Однако установлено, что степень комплаентности пациентов молодого возраста к стоматологическому лечению недостаточно высокая, а кратность обращения к врачу-пародонтологу в 62% ограничивается выраженной стоматофобией. Известно, что развитие пародонтита является результатом дисбаланса между микрофлорой полости рта и иммунной защитой организма. Иммунологические изменения при хроническом катаральном гингивите связаны с нарушением взаимодействия факторов неспецифической резистентности организма, изменением клеточного и гуморального иммунитета и подавлением системы местного иммунитета. Поэтому с целью ликвидации воспаления применяют различные иммунокорректоры и антибактериальные средства (антисептики, антибиотики, фитопрепараты). Однако в последнее время появились формы пародонтита, обусловленные нетипичными инфекционными агентами (вирусами, грибами), либо резистентные к антибактериальной терапии. В результате нерационального применения антимикробных препаратов, которые негативно воздействуют на представителей облигатной микрофлоры полости рта, они еще больше снижают местные факторы антибактериальной защиты. Альтернативой антибактериальной терапии при заболеваниях пародонта являются различные методы биотерапевтического воздействия, предполагающие местное и системное применение пробиотиков, фаговых препаратов и других средств. Вместе с тем, такие исследования в отечественной стоматологии единичные, как и исследования по повышению степени соответствия между поведением пациента и рекомендациями, полученными от врача Treatment of periodontal diseases is one of the most difficult and important problems of modern dentistry, the relevance of which is due to the high frequency of inflammatory periodontal diseases among the population in all age groups - up to 90% in the population. Despite the progressive development of medical science and practice, the incidence of this pathology remains at a high level not only in middle-aged and elderly population groups, but at the same time a tendency towards its spread at a young age is revealed. Studies conducted by WHO in more than 30 countries have revealed a high prevalence of periodontal disease (over 60%) among patients aged 18 to 30 years. The most common form of damage to the periodontal tissues at a young age is chronic gingivitis according to the SKB-10 K 05.1 classification. Irregular prophylaxis and occasional visits to a periodontist for the treatment of periodontitis complicate the course of the disease, which progresses in the absence of treatment. In this regard, the patient is required to undergo repeated courses of treatment with a periodontist and to perform preventive measures at home on the recommendation of a doctor. However, it was found that the degree of compliance of young patients to dental treatment is not high enough, and the frequency of visits to a periodontist in 62% is limited by pronounced stomatophobia. It is known that the development of periodontitis is the result of an imbalance between the microflora of the oral cavity and the body's immune defenses. Immunological changes in chronic catarrhal gingivitis are associated with a violation of the interaction of factors of nonspecific resistance of the organism, changes in cellular and humoral immunity and suppression of the local immunity system. Therefore, in order to eliminate inflammation, various immunocorrectors and antibacterial agents (antiseptics, antibiotics, herbal medicines) are used. However, recently there have been forms of periodontitis caused by atypical infectious agents (viruses, fungi), or resistant to antibiotic therapy. As a result of the irrational use of antimicrobial drugs, which negatively affect the representatives of the obligate microflora of the oral cavity, they further reduce the local factors of antibacterial protection. An alternative to antibiotic therapy for periodontal diseases are various methods of biotherapeutic action, involving the local and systemic use of probiotics, phage preparations and other agents. At the same time, such studies in domestic dentistry are rare, as well as studies to improve the degree of correspondence between the patient's behavior and the recommendations received from the doctor

scholarly journals Application of hirudotherapy in the complex treatment of periodontal diseases

2020 ◽  
Vol 24 (4) ◽  
pp. 273-277
Author(s):  
A. I. Abdullayeva ◽  
A. Y. Bozhedomov ◽  
Elena P. Pustovaya ◽  
V. M. Slonova ◽  
A. S. Karnaeva ◽  
...  
Keyword(s):  
Periodontal Diseases ◽  
Treatment Method ◽  
Bioactive Molecules ◽  
Periodontal Pocket ◽  
Periodontal Tissues ◽  
Periodontal Index ◽  
Noticeable Improvement ◽  
Therapeutic Mechanisms ◽  
Mixed Saliva ◽  
Socio Economic Factors

This article reviews research data on the etiology, pathogenesis, and features of inflammatory periodontal diseases, the possibilities of using hirudotherapy to treat this group of diseases, and the mechanisms of action of this method. The literature search was conducted using the databases Scopus, Web of Science, MedLine, and Global Health. The mechanisms by which medical leeches influence the human body were analyzed. It is known that the progression of inflammatory periodontal diseases depends on risk factors such as age, smoking, gender, socio-economic factors, and some systemic diseases. The main features of chronic and acute periodontitis are periodontal pockets accompanying the loss of bone mass and attachment of the tooth to the alveolar process, which occur unevenly throughout the dentition. The decisive factor in inflammatory periodontal diseases is the presence of microorganisms. Hirudotherapy is a treatment method using the medical leech Hirudo medicinalis. In their secretion, leeches have more than 20 bioactive molecules with various effects. Their therapeutic mechanisms include six subtypes: a decrease in the content of cellular elements and neutrophils in mixed saliva, normalization of the microflora of the periodontal pocket, and an increase in the number of lactobacilli. The treatment method using leeches leads to a decrease in the depth of the periodontal pockets, normalization of the periodontal index, and tooth mobility. Hirudotherapy leads to a noticeable improvement in the clinical condition of periodontal tissues, is convenient to perform, affordable, and can be recommended for use in the practice of periodontists.

scholarly journals THE JUSTIFICATION FOR THE USE OF THE THERAPEUTIC FACTORS OF THE INTERNAL ENVIRONMENT OF MODERN DEVICES MADE OF NATURAL SYLVINITE IN DENTISTRY

2017 ◽  
Vol 72 (3) ◽  
pp. 203-209
Author(s):  
V. A. Chereshnev ◽  
V. G. Barannikov ◽  
L. E. Leonova ◽  
L. V. Kirichenko ◽  
Yu. N. Maslov ◽  
...  
Keyword(s):  
Background Radiation ◽  
Tooth Enamel ◽  
Periodontal Diseases ◽  
Physical Factors ◽  
Therapeutic Effects ◽  
Internal Environment ◽  
Therapeutic Factors ◽  
Local Immunity ◽  
Periodontal Tissues ◽  
The Impact

Background: One of the methods of comprehensive therapeutic effects on the human body is sylvinite therapy which has not been used in the treatment of inflammatory periodontal diseases at young age. Aims: Hygienic and clinical evaluation of the possible application of therapeutic factors of sylvinite devices in dentistry.Materials and methods: We conducted hygienic study of the basic physical factors of the internal environment of modern sylvinite structures (radiation background, aeroionization and aerosol environment, climate). The percentage of sylvine and halite in the sylvinite minerals was determined using color image analysis of the salt screen. The microbiological studies of the impact of the mineral sylvinite on the growth parameters of bacterial culture were performed. We examined 79 patients aged 20−25 years diagnosed with chronic generalized catarrhal gingivitis, 36 persons underwent treatment course in the salt structure. The evaluation of the dental status and the periodontal status was conducted using hygienic and periodontal indices. The duration of study was 4 years.Results: Аll participants who entered the study completed it. In the experimental sylvinite room formed food-grade, biodegradable internal environment (background radiation ― 0,18±0,0027 µsv/h; LOA ― 802,33±62,69 ion/cm3; RO ― 509,33±37,17 ion/cm3; stable microclimate). We detected evidence of an inhibitory effect of the combination on Staphylococcus aureus. The use of a comprehensive dental program has improved the condition of periodontal tissues and careeradvantage tooth enamel (PMA and SBI fell of 80.8% and 75.5%; the reduction of the CPU ― 67.7%; fuel and energy resources increased by 37%). Conclusions: The inclusion of sylvinite therapy in the complex treatment of patients with chronic generalized catarrhal gingivitis had a marked positive impact on the state of periodontal tissues and local immunity of the oral cavity.

scholarly journals Violation of Local Immunity of the Oral Cavity in Agro-Industrial Workers with Generalized Periodontitis under the Influence of Pesticides

2021 ◽  
Vol 6 (1) ◽  
pp. 222-227
Author(s):  
V. T. Dyryk ◽  
◽  
R. V. Shkrebniyuk ◽  
O. M. Vynogradova
Keyword(s):  
Immune System ◽  
Oral Cavity ◽  
Periodontal Diseases ◽  
Main Group ◽  
The State ◽  
Industrial Workers ◽  
Local Immunity ◽  
High Biological Activity ◽  
Periodontal Tissues ◽  
Study Results

Among dental diseases, periodontal diseases occupy a prominent place. The polyetiology and polypathogenicity of periodontal diseases are different in nature: pathological processes at the level of the whole organism, its cells and environments, periodontal tissues, biochemically reactive substrates. The significant impact of adverse environmental factors on the state of human organs and systems is confirmed by the tendency to increase the incidence of periodontal disease in industrialized countries, which in different age groups ranges from 80% to 100%. Pesticides are one of the most important factors determining the well-being and balance of the human-environment system today. This is due to their characteristics as chemical pollutants, which deliberately and purposefully contributes to the environment pollution. The main among these features are constant and excessive scattering over large areas; high biological activity on pets, as well as on humans and other living organisms; the ability to persist in the environment and circulate in its objects; metabolized in biological objects and transformed into the environment with the formation of more toxic and dangerous, compared to the original, compounds; the ability to cause the development of long-term effects. The group of risk of periodontal tissue diseases naturally includes employees of agricultural enterprises, who in the process of work are exposed to occupational pathogens of different nature, intensity and duration of action. Continuous intake of agro-industrial xenobiotics creates a chemical load, causes the occurrence of toxic hypoxia, anemia, imbalance in the immune system, promotes the activation of endogenous intoxication, which in turn is projected on the periodontal tissues, and disease of tooth-retaining tissues. The purpose of our research was to study the state of local immunity of the oral cavity in agro-industrial workers with generalized periodontitis under the influence of pesticides. Material and methods. To determine the changes in local immunity in the oral and gingival fluid we determined the concentrations of lysozyme, sIgA, IgA and IgG in 39 patients with generalized periodontitis who were not adversely affected by greenhouse factors (comparative group) and 81 agricultural workers (41 people in open (I main group) and 40 people in closed soils) II main group), exposed to pesticides). The obtained values were compared with data in 31 dentistically healthy people not in contact with pesticides (control group). Results and discussion. The obtained results showed that generalized periodontitis marked changes in the local immune mechanisms of the oral cavity. However, the most pronounced violations were observed in patients with generalized periodontitis working in closed and open soils under the influence of pesticides, and in subjects who were exposed to toxic effects of chemicals in closed soil, this trend was more intense. Conclusion. The study results proved that agro-industrial workers in contact with pesticides in open and closed soils have significant disturbances in the local immune system, which was characterized by a decrease in the levels of immunoglobulins A, G, sIgA and lysozyme in oral and gingival fluids

Comparative analysis of polymorphic variants of IL-17A and MMP-1 genes with the risk of developing chronic periodontitis in petrochemical workers

2020 ◽  
Vol 60 (10) ◽  
pp. 687-693
Author(s):  
Iskander I. Zaidullin ◽  
Denis O. Karimov ◽  
Lilija K. Karimova ◽  
Milyausha F. Kabirova ◽  
Rasima R. Galimova ◽  
...  
Keyword(s):  
Ethylene Oxide ◽  
Chronic Periodontitis ◽  
Periodontal Diseases ◽  
Clinical Manifestations ◽  
Control Group ◽  
Periodontal Tissues ◽  
Dental Examination ◽  
Number Of Patients ◽  
Increased Risk ◽  
Harmful Substances

The susceptibility to the development and progression of inflammatory periodontal diseases, which depends on genetic and external factors (smoking, stress, oral hygiene), varies widely. In the development of these diseases, an important role is played not only by the presence of periodontal pathogenic microorganisms, but also by the presence of congenital or acquired immunodeficiency, immunoregulatory defects. The immune system plays a key role in the physiological and pathological processes of periodontal tissues. In this regard, IL17, produced by CD4+ Th cells, which has both Pro-inflammatory and protective activity, is of particular interest in the pathogenesis of periodontitis. The aim of study was to identify the relationship between polymorphic loci of the IL-17A (rs2275913) and MMP-1 (rs1799750) genes and clinical manifestations of chronic periodontitis in petrochemical workers. Dental examination was performed in 92 ethylene oxide production workers with chronic periodontitis and 74 patients with chronic periodontitis who did not come into contact with chemical factors (control group). Genotyping of polymorphisms rs2275913 of the IL17A gene and rs1799750 of the MMP1 gene was performed by allele-specific real-time polymerase chain reaction (PCR). Hygienic assessment of the degree of air pollution of the working area with harmful substances was carried out by gas chromatography according to the guidelines for the determination of harmful substances in the air № 5098-89, № 3119-84. When comparing the results of studies of both groups, there were no statistically significant differences in the frequency distributions of allelic variants and genotypes of the IL-17A and MMP-1 genes. The AA/AG genotypes of the IL-17A gene were associated with an increased risk of severe disease compared to the GG genotype in workers in the main group (OR=6.1; 95% CI 1.33-28.5; p=0.021) and in the control group (OR=7.26; 95% CI 1.34-39.25; p=0.016). Carriers of the A allele in the control group increased the risk of severe chronic periodontitis by 2.4 times compared to carriers of the G allele (OR=2.41; 95% CI 1.19-4.87; p=0.014). During the dental examination of employees of the ethylene oxide plant, the clinical course of periodontal diseases was more severe in comparison with the control group, and the number of patients with severe periodontitis was twice as high. It was found that the AA/AG genotypes of the IL-17A gene and the carrier of the A allele are associated with increased susceptibility to the development of severe chronic periodontitis. The association between the MMP-1 gene polymorphism and the risk of severe forms of chronic periodontitis has not been established. A risk factor for the development of inflammatory periodontal diseases in employees of the petrochemical complex is a complex of harmful production factors.

Sign in / Sign up

Export Citation Format

Share Document