scholarly journals ANTIBACTERIAL ACTIVITY IN EARTHWORM COELOMIC FLUID LAMPITO MAURITII

2016 ◽  
Vol 3 (2) ◽  
pp. 89-93
Author(s):  
Deepthi M.P ◽  
Kathireswari P
Keyword(s):  
Carbon Dioxide ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Coelomic Fluid ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Zone Formation ◽  
Micro Organisms

Earthworms are the first annulated coelomate organism with haemoglobin in the plasma as carrier of oxygen and carbon dioxide. They have the antimicrobial activity against the disease causing micro organisms. In the present study antibacterial activity of earthworm coelomic fluid Lampito mauritii were confinedagainst disease causing micro organisms through the inhibition zone formation by well diffusion method as well as disc diffusion method.

scholarly journals Synthesis and Antimicrobial Activity of Piperine Analogues Containing 1,2,4-Triazole Ring

2019 ◽  
Vol 31 (5) ◽  
pp. 1077-1080
Author(s):  
Kottakki Naveen Kumar ◽  
Karteek Rao Amperayani ◽  
V. Ravi Sankar Ummdi ◽  
Uma Devi Parimi
Keyword(s):  
Antimicrobial Activity ◽  
Triazole Ring ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Antibacterial Study ◽  
Gram Negative ◽  
E Coli ◽  
Micro Organisms

A series 1,2,4-triazole piperine analogues (TP1-TP6) were designed and synthesized. The structures were confirmed using 1H NMR and 13C NMR. Antibacterial study was done using Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative micro-organisms (E. coli and Pseudomonas aeruginosa) by disc diffusion method. Compound containing chloro substitution (TP6) showed the highest effect, while compound TP1, TP3, TP4, TP5 showed the moderate activity.

scholarly journals Chemical Composition and Antibacterial Activity of Essential Oil of Anvillea radiata Coss. & Dur

2007 ◽  
Vol 2 (5) ◽  
pp. 1934578X0700200
Author(s):  
Fadwa El Hanbali ◽  
Ahmed El Hakmaoui ◽  
Fouad Mellouki ◽  
Lahoussine El Rhaffari ◽  
Mohamed Akssira
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Aerial Parts

The essential oil of the aerial parts of Anvillea radiata Coss. & Dur. was examined by GC-MS. Twenty-nine constituents were identified, representing 88.8% of the total. 6-Oxocyclonerolidol (66.6%) and 6-hydroxycyclonerolidol (11.4%) were found to be the major components. The disc diffusion method, used for antimicrobial activity testing, showed that the essential oil had a significant antibacterial activity against all tested microorganisms.

scholarly journals Antimicrobial Activity of some Honey Samples against Pathogenic Bacteria

2015 ◽  
Vol 34 ◽  
pp. 15-20 ◽  
Author(s):  
S. Mahendran ◽  
D. Kumarasamy
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Western Ghats ◽  
Pathogenic Bacteria ◽  
Research Work ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method

The aim of the present research work to investigate antimicrobial activity of some honey samples six winter honeys six summer honeys collected from different regions of Western Ghats. The microbes used in this study are Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis. Antibacterial activity of the honeys was assayed using the Disc diffusion method. Noticeable variations in the antibacterial activity of the different honey samples were observed. Among the microbes Staphylococcus aureus is the most sensitive against all honey samples shows the maximum inhibitor zone compare to summer honeys.

scholarly journals Formulation and Antibacterial Activity of Liquid Soap Containing Ketapang (Terminalia catappa L.) Leaves Extract

2021 ◽  
Vol 4 (1) ◽  
pp. 43-50
Author(s):  
Asiska Permata Dewi ◽  
Dini Mardhiyani
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Foam Stability ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Liquid Soap ◽  
Disc Diffusion Method ◽  
Inhibition Zone Diameter ◽  
Terminalia Catappa

Ketapang (Terminalia catappa L.) is traditionally used by the community to treat infections of the skin caused by bacteria or fungi. In this study, T. catappa leaves extract was added to the liquid soap formula as an antibacterial. The purpose of this study was to determine the secondary metabolite compounds contained in T. catappa leaves extract, physical evaluation of the preparation, and antibacterial activity of liquid soap. Liquid soap formula is made with various concentrations of T. catappa leaves extract F0 (0%), F1 (1%), F2 (2%), and F3 (3%). The resulting soap was evaluated for organoleptic, pH, high foam, homogeneity, irritation, and its activity against Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli using the disc diffusion method. The results showed that the T. catappa leaves extract contained flavonoids, tannins, saponins, and triterpenoids. The liquid soap formula F0 is clear, while F1, F2, and F3 have the characteristics of brown-dark brown, homogeneous, pH between 4.6-5.2, foam stability between 67-72%, which is not significantly different and stable after five minutes of testing, and it does not irritate the skin. Terminalia catappa leaves extracts liquid soap has antibacterial activity at a concentration of 1%, 2%, and 3%, with the largest inhibition zone diameter produced by S. aureus.

scholarly journals AKTIVITAS ANTIMIKROBA BAKTERI ASAM LAKTAT YANG DIISOLASI DARI MAKANAN TRADISIONAL FERMENTASI KHAS BATAK “NANIURA”

2019 ◽  
Vol 6 (1) ◽  
pp. 8
Author(s):  
Aloysius Aloysius ◽  
Anjurniza Ulfa ◽  
Anggita Kasih Fianti Situmorang ◽  
Harmileni Harmileni ◽  
Edy Fachrial
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Fermented Food ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
E Coli ◽  
Gram Staining ◽  
Potential Source

Lactic acid bacteria (LAB) could be isolated from various fermented food products. One potential source of LAB is traditional fermented food. The aim of the study was to isolate and investigate antimicrobial activity of LAB isolated from traditional Batak food, “naniura”. The LAB isolates were characterized by Gram staining, fermentation type and catalase test. The investigation of antimicrobial activity of LAB against pathogenic bacteria were conducted using disc diffusion method. The results showed that 6 isolates of BAL were successfully isolated namely BN1, BN2, BN3, BN4, BN5 and BN6 had characteristics of Gram positive, rod shaped and catalase negative. All selected isolate have heterofermentation type. Four isolates (BN1, BN2, BN5 and BN6) were able to inhibit S. aureus, E. coli and S. typhi with inhibition zone diameters ranging from 6,9 to 12,3 cm. Based on the result, it was concluded that LAB isolated from naniura has potential as a source of probiotics.

scholarly journals ANTIBACTERIAL ACTIVITY OF A COPPER(II) COMPLEX OF 1-(1H-BENZIMIDAZOL-2-YL)-N- (TETRAHYDROFURAN-2-YLMETHYL) METHANAMINE

2015 ◽  
Vol 2 (1) ◽  
pp. 11-14
Author(s):  
Elayaperumal R ◽  
Kiruthika M ◽  
Dharmalingam P
Keyword(s):  
Antimicrobial Activity ◽  
Cyclic Voltammetry ◽  
Antibacterial Activity ◽  
Free Ligand ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Infra Red ◽  
Uv Visible

A mononuclear copper(II) complex of 1-(1H-benzimidazol-2-yl)-N-(tetrahydrofuran-2- ylmethyl)methanamine) was synthesized and characterized by various physicochemical techniques like cyclic voltammetry and elemental analysis, ESI-MS, UV–Visible, Infra red and EPR spectroscopy. The antibacterial activities of the ligand and its complex were screened by disc diffusion method and found that the metal complexes have higher antimicrobial activity than the free ligand.

scholarly journals Evaluation of the antibacterial activity of bergamot essential oils on different Listeria monocytogenes strains

2016 ◽  
Vol 5 (4) ◽  
Author(s):  
Stefania M. Marotta ◽  
Filippo Giarratana ◽  
Alessio Parco ◽  
Domenico Neri ◽  
Graziella Ziino ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Listeria Monocytogenes ◽  
Essential Oils ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Strong Activity ◽  
Agar Disc ◽  
Inhibition Experiment

Essential oils are aromatic and volatile substances extracted from plants and characterized by antimicrobial activity. The aim of the present study was to evaluate the antibacterial activity (agar disc-diffusion method) of seven different bergamot essential oils (BEOs) on eight <em>Listeria monocytogenes</em> strains. Minimal inhibitory concentration (MIC) of most efficient BEOs was estimated. Extremely variable results for agar disc-diffusion method for <em>L. monocytogenes</em> strains were reported. One of the tested microorganisms resulted insensible to all the BEOs; 3 strains showed an inhibition from weak to null and the remaining 4 a variable susceptibility. Among the BEOs tested, one showed a strong activity against four pathogenic strains. Four BEOs revealed weak, moderate or null activity in all the 7 sensitive strains, while for two oils only a weak or no activity was reported. MIC values were 0.625 μL/mL for the most efficient BEO, 2.5 and 5 μL/mL for the other samples that showed moderate inhibition. Experiment results are significantly related to the strains tested (P&lt;0.01), rather than the BEO employed (P&gt;0.01). In conclusion, we can consider BEO as a natural technological hurdle for <em>Listeria monocytogenes</em> in combination with other preservation strategies. Finally, this study underlines the necessity to evaluate the antimicrobial activity of EOs on a significant strains number of the same bacteria.

scholarly journals Antibacterial activities of Protein Extracts From Andrographis Paniculata, Tinospora Crispa and Centella Asiatica

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Noor Hasniza Zin ◽  
Nurul Fatihah Ahmad ◽  
Noraslinda Muhamad Bunnori ◽  
Widya Abdul Wahab ◽  
Normah Haron
Keyword(s):  
Antibacterial Activity ◽  
Centella Asiatica ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Andrographis Paniculata ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Sds Page ◽  
New Antibiotics

Introduction: Andrographis paniculata, Tinospora crispa and Centella asiatica are known to have various pharmacological functions. This research was carried out to investigate the antibacterial activities of protein extracts from A. paniculata, T. crispa and C. asiatica. Methods: Total soluble proteins from these herbs were extracted using a modified TCA/acetone method. The protein extracts were then quantified using the Bradford assay and separated using SDS-PAGE. The antibacterial activities were determined by disc diffusion method. Results: T. crispa had a significantly higher amount of proteins (83.86 ± 0.4 µg/µl) compared to A. paniculata (81.57 ± 0.4 µg/µl) and C. asiatica (78.93 ± 0.5 µg/µl). The proteins separated by SDS-PAGE were ranged from 30kDa to 260kDa, 25kDa to 110kDa and 25kDa to 160kDa for A. paniculata, T. crispa and C. Asiatic, respectively. The high abundance proteins were observed in A. paniculata and T. crispa but not in C. asitica. Protein extracts from C. asiatica have demonstrated antibacterial activity against all tested bacteria with the diameter of inhibition zone of 11.0 ± 0.5 mm, 12.3 ± 0.6 mm, 10.7 ± 0.7 mm and 20.0 ± 0.8 mm against B. cereus, S. aureus, K. pneumonia and S. typhimurium respectively. Meanwhile, protein extracts of A. paniculata showed a positive antibacterial activity only against B.cereus (13.7 ± 0.4 mm), S. aureus (7.0 ± 0.8 mm) and S. typhimurium (11.5 ± 0.3 mm). Protein extracts from T. crispa only showed a positive antibacterial activity against B. cereus (9.7 ± 0.5 mm). Conclusions: There is a constant need in the discovery of new antibiotics for the treatment of infectious diseases.

scholarly journals POTENSI EKSTRAK TUMBUHAN ANDALIMAN (Zanthoxylum acanthopodium DC.) SEBAGAI ANTIBAKTERI Staphylococcus aureus

Alotrop ◽  
2019 ◽  
Vol 2 (2) ◽  
pp. 133-139
Author(s):  
Oma Sepriani ◽  
Nurhamidah Nirhamidah ◽  
Dewi Handayani
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Secondary Metabolite ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Activity Test ◽  
North Sumatra

The aims of this study is to determine the secondary metabolite compounds in Roots, stem bark, leaves and fruit of Andaliman (Zanthoxylum acanthopodium DC), and determine the most potent part of Andaliman plant extract in inhibiting the growth of Staphylococcus aureus bacteria. Andaliman plant taken in Simarpingan, Sipaholon village sub district of North Sumatra. Part of the Andaliman plantar are cleaned, cut into small pieces and then dried and mashed, samples that have been finely phytochemically tested and extracted. Testing of secondary metabolite compound by doing phytochemical screening of roots, stem bark, leaves and fruit of Andaliman, alkaloid test using meyers reagent, terpenoid test and steroid using TLC plate with eluent n-hexana : ethyl acetate with ratio 6:4, flavonoid test using soluton HCl and Mg band, saponin test using soap test, tannin test using ferro (III) chloride solution. Test of antibacterial activity using disc diffusion method. Phytochemical results showed that all parts of the plant andaliman contain secondary metabolite compounds namely alkaloids, flavonoids, terpenoids, tannins and also saponins. The result of antibacterial activity test of leaf extract of leaf and leaf which has the most potential as antibacterial of S.aureus is bark. Because it able to inhibit S.aureus growth with 11 mm inhibition zone at concentration 5x104 ppm, which is included in the strong category. In addition to skin of andaliman leaf stem is also potential as an antibacterial with a 10 mm inhibition zone at concentration 5x104 ppm, which is included in the medium category.

scholarly journals AKTIVITAS ANTIBAKTERI INFUSA DAUN LIDAH BUAYA (Aloe vera L) TERHADAP PERTUMBUHAN Staphylococcus aureus DAN Escherichia coli

2019 ◽  
Vol 6 (1) ◽  
pp. 33
Author(s):  
Yuni Widyastuti ◽  
Nia Yuliani ◽  
I.G.A. Manik Widhyastini
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Aloe Vera ◽  
Bacterial Count ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Escherichia Coli Bacteria

Antibacterial Activity of Infused Aloe Vera Leaf (Aloe Vera L) on Growth Of Stapylococcus aureus and Escherichia coliDisinfectants may act as antibacterials. Aloe vera (Aloe vera L) is one of the plants that is often used by the community as a medicinal plant that has many active compounds such as lignin, saponin and anthraquinone substances that act as antibacterial. This study aims to determine the antibacterial activity of Aloe vera leaf extracted with infusa method on the growth of Staphylococcus aureus and Escherichia coli bacteria. The research method is to see the reduction / reduction of bacterial count (% reduction) after 30 second and 60 second with disc diffusion method. The results showed decrease or decrease of bacteria count (% reduction) to Escherichia coli bacteria found in aloe leaf skin infected by 28,2% after 60 seconds. While decrease or decrease of bacteria amount (% reduction) to Staphyloccocus uareus bacteria infusa mixture of meat and skin of Aloe vera leaves of 27.5% after 60 seconds. Antibacterial test of aloe vera leaf aloe (Aloe veraL) of 100% concentration by disc diffusion method did not show any inhibition zone around cultures of test bacteria.Keywords: Aloe vera, antibacterial activity, Staphylococcus aureus, and Escherichia coli, Infusa.ABSTRAKDesinfektan dapat berfungsi sebagai antibakteri. Lidah buaya (Aloe vera L) merupakan salah satu tanaman yang sering digunakan masyarakat sebagai tanaman obat yang memiliki banyak senyawa aktif seperti lignin, saponin dan antrakuinon yaitu zat yang berfungsi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri daun lidah buaya (Aloe vera L) yang diekstrak dengan metode infusa terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Metode penelitian yaitu dengan melihat penurunan/pengurangan jumlah bakteri (% reduksi) setelah waktu 30 detik dan 60 detik dengan metode difusi cakram. Hasil penelitian menunjukkan penurunan atau pengurangan jumlah bakteri (% reduksi) terhadap bakteri Escherichia coli terdapat pada infusa kulit daun lidah buaya yaitu sebesar 28,2 % setelah waktu 60 detik.Sedangkan penurunan atau pengurangan jumlah bakteri (% reduksi) terhadap bakteri Staphyloccocus uareus terdapat pada infusa campuran daging dan kulit daun lidah buaya yaitu sebesar 27,5 % setelah waktu 60 detik. Uji antibakteri infusa daun lidah buaya (Aloe veraL) konsentrasi 100% dengan metode difusi cakram tidak menunjukkan adanya zona penghambatan disekitar biakan bakteri uji.Kata kunci : Lidah buaya, Aktivitas antibakteri, Staphylococcus aureus, dan   Escherichia coli,   Infusa.

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