scholarly journals Impact of the small bowel impassability on expression of the hypoxia-induced factor-1α

2021 ◽  
Vol 88 (5-6) ◽  
pp. 65-68
Author(s):  
D. A. Kluiko
Keyword(s):  
Small Bowel ◽  
Partial Pressure ◽  
Process Development ◽  
Abdominal Cavity ◽  
Cellular Structures ◽  
Laboratory Animals ◽  
Partial Pressure Of Oxygen ◽  
Visceral Peritoneum ◽  
Disease Occurrence ◽  
The Impact

Objective. Studying of the hypoxia-induced factor-1α expression and peritoneal oxygenation in experimental small bowel intestinal impassability. Materials and methods. The investigation was done on 18 laboratory animals, in which 62 metrics of direct oxymetry performed with the objective to study the impact of the small bowel impassability on velocity of the mass transduction and partial pressure of oxygen and the hypoxia-induced factor-1α expression, influenced by hypoxia. Results. In experimental ileus, complete or partial one, partial pressure and velocity of the oxygen mass-transduction in peritoneum were statistically significantly lower, than such in a control, indicating on inhibition of cellular structures, utilizing the oxygen, what promotes the adhesive disease occurrence. Experimental ileus have led to lowering of the oxygen partial pressure in peritoneum by 88% - from 28.7 (in a control) to 3.5 mm Hg (p<0.01). The partial (by 277.8%) and the complete (by 248.2%) experimental ileus was characterized by enhancement of the hypoxia-induced factor-1α expression. Conclusion. Ileus, accompanied by change in the parietal and visceral peritoneum oxygenation, stimulates the abdominal cavity adhesive process development.

scholarly journals Effect of intra-abdominal hypertension on the strength of the postoperative scar of a laparotomic wound

2020 ◽  
Vol 24 (2) ◽  
pp. 223-226
Author(s):  
I. K. Morar
Keyword(s):  
Mechanical Strength ◽  
Negative Impact ◽  
Abdominal Cavity ◽  
Study Groups ◽  
Laboratory Animals ◽  
Abdominal Pressure ◽  
Abdominal Hypertension ◽  
Laparotomy Wound ◽  
The Impact ◽  
Postoperative Scar

Annotation. One of the main factors of postoperative eventration is intra-abdominal hypertension, which occurs in various surgical pathologies of the abdominal cavity. Despite the presence of a large number of scientific papers on the negative effect of intra-abdominal hypertension on the morphological state of granulation tissue in the area of the laparotomy wound, there are no publications on the impact on the strength of the postoperative scar. Therefore, the study aimed to investigate in an experiment on small laboratory animals the effect of intra-abdominal hypertension on the mechanical strength of the postoperative scar of a laparotomy wound. The experiment was performed on 120 laboratory rats, which underwent a median laparotomy and brought together the edges of the musculoaponeurotic layer of the anterior abdominal wall with simple nodal sutures. The main group consisted of 72 animals who developed intra-abdominal hypertension by inserting a container (condom) with a certain amount of Furacilin into the abdominal cavity. The comparison group consisted of 48 animals who had an empty condom inserted into the abdominal cavity after laparotomy. The mechanical strength of the postoperative scar of the laparotomy wound was determined by the method of G. V. Petrovich (2010) on the 1st, 3rd, and 5th day after the creation of intra-abdominal hypertension, by measuring the level of intra-abdominal pressure at the time of rupture of the postoperative scar of the laparotomy wound. Statistical analysis of the results was performed using Microsoft Excel spreadsheets and a package of statistical processing software PAST. Differences between study groups were determined using Mann-Whitney criteria. The results of the study indicate that the created intra-abdominal hypertension leads to a decrease in the mechanical strength of the postoperative scar of the laparotomy wound. The degree of the negative impact of intra-abdominal hypertension on the strength of the postoperative scar is inversely proportional to the level of intra-abdominal pressure.

scholarly journals REGISTRATION OF INDICATORS OF TISSUE RESPIRATION IN THE INTESTINAL WALL DURING ADHESION PROCESS IN EXPERIMENT

2021 ◽  
pp. 38-43
Author(s):  
D.A. Klyujko ◽  
◽  
V.E. Korik ◽  
Keyword(s):  
Rapid Development ◽  
Abdominal Cavity ◽  
Experimental Studies ◽  
Clinical Manifestations ◽  
Intestinal Wall ◽  
Laboratory Animals ◽  
Tissue Respiration ◽  
Surgical Interventions ◽  
Peritoneal Tissue ◽  
The Impact

The problem of adhesion after surgical diseases on the abdominal organs remains extremely relevant despite the rapid development of new medical technologies and approaches to the diagnosis and treatment of surgical diseases. The formation of adhesions occurs during open surgical interventions and will differ only in the degree of severity and clinical manifestations. The article is devoted to the study of oxygenation of the peritoneal tissue in experimental adhesions of the abdominal cavity. Experimental studies were carried out on 95 outbred guinea pigs with simulated adhesive disease of the abdominal cavity. As a result of performing direct oximetry on the surface of the peritoneum in conditions of modeling adhesions of the abdominal cavity in laboratory animals, it was revealed that any of the models causes, to a greater or lesser degree, hypoxic changes in the peritoneum, thereby creating favorable conditions for the maturation of coarse-fibrous connective tissue. The impact on the peritoneum of any of the modeling factors caused a decrease in the partial pressure of oxygen in the intestinal wall by an average of 45%. The leveling of hypoxic changes on the surface of the peritoneum in the postoperative period is a method of preventing adhesions of the abdominal cavity.

scholarly journals Possibilities of clinical application of nano-crystal hydroxyapatyte in treatment of purulent-inflammatory diseases

2021 ◽  
Vol 88 (5-6) ◽  
pp. 75-80
Author(s):  
І. D. Duzhyi ◽  
L. F. Sukhodub ◽  
V. S. Bielai ◽  
O. M. Lytvynenko ◽  
A. V. Yurchenko
Keyword(s):  
Zinc Oxide ◽  
Body Mass ◽  
Abdominal Cavity ◽  
Drainage System ◽  
Physiological Solution ◽  
The Body ◽  
Laboratory Animals ◽  
Antiinflammatory Action ◽  
Group I ◽  
The Impact

Objective. To investigate the impact of the sorbtion-aspiration drainage system on background of hydroxyapatite and zinc oxide on experimental peritonitis. Materials and methods. The experiment was performed on 55 white laboratory rat males, ageing 2 - 3 mo, with the body mass 225 - 250 g, which were kept in the typical vivarium conditions. Suspension of E. coli in physiological solution, 1.0 - 1.5 ml in concentration 1010 , was used for the peritoneum contamination. The suspension was introduced into abdominal cavity in the dose, dependent on the animal body mass, using the syringe for insulin injection. Peritonitis in the rats was developed in 48 h and characterized by intoxication, which exaggerated rapidly, as well as by intestinal motor disorders and pronounced microcirculatory disturbances. The experimental animals were distributed into 5 Groups. In patients of Group I the interventions were not performed (control); in laboratory animals of Group II peritonitis was not simulated, but the abdominal cavity was drained, using the sorption-aspiration drainage system; in laboratory animals of Group III peritonitis was simulated; in the Group IV of laboratory animals on background of peritonitis the abdominal cavity was drained, using the sorption-aspiration drainage system; in the Group V animals the abdominal cavity was drained by a standard silicon drain on background of peritonitis. Results. Reduction of abdominal contamination by 43.12% and of histological features were produced in abdominal organs under the influence of the composite system, witnessing its bactericidal and sorption-antiinflammatory action. Conclusion. Experimentally there was confirmed the possibility of application of sorption-aspiration drainage system on background of hydroxyapatite and zinc oxide in treatment of an acute peritonitis.

Interaction of H2O, O2 and H2 with Proton Conducting Oxides Based on Lanthanum Scandates

2019 ◽  
pp. 88-100
Author(s):  
A. S. Farlenkov ◽  
N. A. Zhuravlev ◽  
Т. A. Denisova ◽  
М. V. Ananyev
Keyword(s):  
Water Vapor ◽  
Partial Pressure ◽  
Gas Phase ◽  
Molecular Hydrogen ◽  
Proton Magnetic Resonance ◽  
Partial Pressure Of Oxygen ◽  
Proton Conducting ◽  
Conducting Oxides ◽  
Temperature Range ◽  
Apparent Level

The research uses the method of high-temperature thermogravimetric analysis to study the processes of interaction of the gas phase in the temperature range 300–950 °C in the partial pressure ranges of oxygen 8.1–50.7 kPa, water 6.1–24.3 kPa and hydrogen 4.1 kPa with La1–xSrxScO3–α oxides (x = 0; 0.04; 0.09). In the case of an increase in the partial pressure of water vapor at a constant partial pressure of oxygen (or hydrogen) in the gas phase, the apparent level of saturation of protons is shown to increase. An increase in the apparent level of saturation of protons of the sample also occurs with an increase in the partial pressure of oxygen at a constant partial pressure of water vapor in the gas phase. The paper discusses the causes of the observed processes. The research uses the hydrogen isotope exchange method with the equilibration of the isotope composition of the gas phase to study the incorporation of hydrogen into the structure of proton-conducting oxides based on strontium-doped lanthanum scandates. The concentrations of protons and deuterons were determined in the temperature range of 300–800 °C and a hydrogen pressure of 0.2 kPa for La0.91Sr0.09ScO3–α oxide. The paper discusses the role of oxygen vacancies in the process of incorporation of protons and deuterons from the atmosphere of molecular hydrogen into the structure of the proton conducting oxides La1–xSrxScO3–α (x = 0; 0.04; 0.09). The proton magnetic resonance method was used to study the local structure in the temperature range 23–110 °C at a rotation speed of 10 kHz (MAS) for La0.96Sr0.04ScO3–α oxide after thermogravimetric measurements in an atmosphere containing water vapor, and after exposures in molecular hydrogen atmosphere. The existence of proton defects incorporated into the volume of the investigated proton oxide from both the atmosphere containing water and the atmosphere containing molecular hydrogen is unambiguously shown. The paper considers the effect of the contributions of the volume and surface of La0.96Sr0.04ScO3–α oxide on the shape of the proton magnetic resonance spectra.

B-cell and T-cell values in peripheral blood in Polish mixed-breed rabbits with addition of blood of meet breeds

2014 ◽  
Vol 17 (3) ◽  
pp. 421-426 ◽  
Author(s):  
B. Tokarz-Deptuła ◽  
P. Niedźwiedzka-Rystwej ◽  
B. Hukowska-Szematowicz ◽  
M. Adamiak ◽  
A. Trzeciak-Ryczek ◽  
...  
Keyword(s):  
T Cells ◽  
B Cells ◽  
B Cell ◽  
Cd8 T Cells ◽  
Peripheral Blood ◽  
Laboratory Animals ◽  
Immunological Parameters ◽  
Four Seasons ◽  
Mixed Breed ◽  
The Impact

Abstract In Poland, rabbit is a highly valued animal, due to dietetic and flavour values of its meat, but above all, rabbits tend to be commonly used laboratory animals. The aim of the study was developing standards for counts of B-cells with CD19+ receptor, T-cells with CD5+ receptor, and their subpopulations, namely T-cells with CD4+, CD8+ and CD25+ receptor in the peripheral blood of mixed-breed Polish rabbits with addition of blood of meet breeds, including the assessment of the impact of four seasons of the year and animal sex on the values of the immunological parameters determined. The results showed that the counts of B- and T-cells and their subpopulations in peripheral blood remain within the following ranges: for CD19+ B-cells: 1.05 - 3.05%, for CD5+ T-cells: 34.00 - 43.07%, CD4+ T-cells: 23.52 - 33.23%, CD8+ T-cells: 12.55 - 17.30%, whereas for CD25+ T-cells: 0.72 - 2.81%. As it comes to the season of the year, it was observed that it principally affects the values of CD25+ T-cells, while in the case of rabbit sex, more changes were found in females.

Biorhythm of the partial pressure of oxygen in uterine and fetal tissues

1981 ◽  
Vol 92 (4) ◽  
pp. 1305-1307
Author(s):  
A. Ya. Chizhov ◽  
V. G. Filimonov ◽  
Yu. M. Karash ◽  
R. B. Strelkov
Keyword(s):  
Partial Pressure ◽  
Partial Pressure Of Oxygen ◽  
Fetal Tissues

scholarly journals Viscoelastic Properties of Cell Structures Manufactured Using a Photo-Curable Additive Technology—PJM

Polymers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1895
Author(s):  
Tomasz Kozior ◽  
Czesław Kundera
Keyword(s):  
Viscoelastic Properties ◽  
Cellular Structure ◽  
Material Selection ◽  
Cellular Structures ◽  
Test Results ◽  
Additive Technology ◽  
Polymer Resin ◽  
Cell Structures ◽  
Relaxation Curves ◽  
The Impact

This research paper reviews the test results involving viscoelastic properties of cellular structure models made with the PolyJet Matrix—PJM additive technology. The designed test specimens were of complex cellular structure and made of three various photo-curable polymer resin types. Materials were selected taking into account the so-called “soft” and “tough” material groups. Compressive stress relaxation tests were conducted in accordance with the recommendations of standard ISO 3384, and the impact of the geometric structure shape and material selection on viscoelastic properties, as well as the most favorable geometric variants of the tested cellular structure models were determined. Mathematica and Origin software was used to conduct a statistical analysis of the test results and determine five-parameter functions approximating relaxation curves. The most favorable rheological was adopted and its mean parameters determined, which enables to match both printed model materials and their geometry in the future, to make a component with a specific rheological response. Furthermore, the test results indicated that there was a possibility of modelling cellular structures within the PJM technology, using support material as well.

scholarly journals Inadequate management of natural ecosystem in the Brazilian Amazon region results in the emergence and reemergence of arboviruses

2001 ◽  
Vol 17 (suppl) ◽  
pp. S155-S164 ◽  
Author(s):  
Pedro F. C. Vasconcelos ◽  
Amélia P. A. Travassos da Rosa ◽  
Sueli G. Rodrigues ◽  
Elizabeth S. Travassos da Rosa ◽  
Nicolas Dégallier ◽  
...  
Keyword(s):  
Environmental Changes ◽  
Epidemiological Data ◽  
Brazilian Amazon ◽  
Host Population ◽  
Amazon Region ◽  
Laboratory Animals ◽  
Natural Ecosystem ◽  
Brazilian Amazon Region ◽  
Mining Dam ◽  
The Impact

A total of 187 different species of arboviruses and other viruses in vertebrates were identified at the Evandro Chagas Institute (IEC) from 1954 to 1998, among more than 10,000 arbovirus strains isolated from humans, hematophagous insects, and wild and sentinel vertebrates. Despite intensive studies in the Brazilian Amazon region, especially in Pará State, very little is known about most of these viruses, except for information on date, time, source, and method of isolation, as well as their capacity to infect laboratory animals. This paper reviews ecological and epidemiological data and analyzes the impact of vector and host population changes on various viruses as a result of profound changes in the natural environment. Deforestation, mining, dam and highway construction, human colonization, and urbanization were the main manmade environmental changes associated with the emergence and/or reemergence of relevant arboviruses, including some known pathogens for humans.

Resistless Patterning of Magnetic Storage Media Using Ion Projection Structuring

2001 ◽  
Vol 705 ◽  
Author(s):  
A. Dietzel ◽  
R. Berger ◽  
H. Grimm ◽  
C. Schug ◽  
W. H. Bruenger ◽  
...  
Keyword(s):  
Process Development ◽  
Ion Beam ◽  
Silicon On Insulator ◽  
Magnetic Storage ◽  
Magnetic Islands ◽  
Superlattice Structure ◽  
Head Positioning ◽  
Out Of Plane ◽  
Set Up ◽  
The Impact

AbstractCo/Pt thin film multilayers with strong perpendicular anisotropy and out-of-plane coercivities of 5-11 kOe were magnetically altered in areas of local ion beam interaction. The ion irradiations were performed by ion projection through silicon stencil masks fabricated by silicon on insulator (SOI) membrane technology. The ion projector at the Fraunhofer Institute for Silicon Technology (ISiT) was operated at 73 keV ion energy and with a 8.7- fold demagnification. After exposure to 3 × 1014Ar+/ cm2 magnetic islands smaller than 100 nm in diameter were resolved in the Co/Pt multilayersby means of magnetic force microscopy. The impact of different ion species (He+, Ar+ and Xe+) and ion energies (10 – 200 keV) on the multilayer structure was evaluated using Monte Carlo simulations. The ballistic interface intermixing was used to predict magnetic coercivity changes for various irradiation conditions. The simulations revealed that with 73 keV Ar+ and Xe+ ions the irradiation dose could be reduced by a factor of 100 and 400 respectively in comparison to 73 keV He+which was verified in the experiments. X-ray reflectivity measurements confirmed that the Co/Pt superlattice structure is slightly weakened during the irradiation and that the surface smoothness of the media is preserved. Using the Ion Projection Process Development Tool (PDT) at IMS-Vienna concentric data tracks including head positioning servo informations were patterned onto a 1” IBM microdrive™ glass disk which was coated with Co/Pt multilayers. In a single exposure step several tracks within an exposure field of 17 mm in diameter were structured by 2 × 1015He+/ cm2 at 45 keV using a 4- fold demagnification set-up.

Sign in / Sign up

Export Citation Format

Share Document