scholarly journals Effect of Obesity on Serum Estrogen concentration and ovarian growth with uterine development in Mature Female Wistar Rats

2012 ◽  
Vol 11 (2) ◽  
pp. 162
Author(s):  
H.A.N. AL-Zamely
Keyword(s):  
Wistar Rats ◽  
Treated Group ◽  
Mature Female ◽  
Female Rats ◽  
Control Female ◽  
Abdominal Vein ◽  
Significant Decrement ◽  
Female Wistar Rats ◽  
Animal House ◽  
Estrogen Concentration

The present study has been carried out at the College of Veterinary Medicine ,AL-Qadisiya University to determine the effect of obesity on serum Estrogen concentration in mature female Wistar rats.Fifty immature female rats (14-20 days age,30-40g.) were housed at the animal house during the period extended from 25th March,2011 to 10th June,2011.Animals were randomly divided into two equal groups. Control fed on standard feed and treated fed on high fat feed ( 32.3%fat by weight and 4828 k cal/kg energy). Daily body weights were recorded during the experiment period extended to the 100th day of age .At 60 days of age ,eight of mature female Wistar rats from each group were anesthetized, dissected and blood samples were obtained from abdominal vein for assessment of Estrogen concentration in serum. Ovaries and uteri were also obtained, weighted and fixed in formalin 10% for histopathological study.Ovarian and uterine weights of treated group recorded significant decrement in comparison with that of control.Hormonal assay in sera showed significant decrement of estrogen in treated mature female rats in comparison with that of control female rats. Ovarian sections of treated female rats revealed lower level of proliferation in the follicular tissue and predominance of Graffian follicles compared with control. Similarly uterine sections of treated female rats showed filtration of adipose tissue with less proliferated changes and less uterine glands compared with that in control.

scholarly journals Estrogenic Effect of Bambusa arundinacea, Trichosanthes dioica and Punica granatum on Rats

2022 ◽  
Author(s):  
Akanksha Awasthi ◽  
Mamta F. Singh ◽  
Saurabh Sharma
Keyword(s):  
Wistar Rats ◽  
Estrogenic Activity ◽  
Ethanolic Extract ◽  
Punica Granatum ◽  
Mature Female ◽  
Female Rats ◽  
Vaginal Opening ◽  
Estrogenic Effect ◽  
Female Wistar Rats ◽  
Trichosanthes Dioica

Background: Phytoestrogens have recently become a hot topic among scientists. Phytoestrogens’ estrogen-like properties have led to their widespread use in the reproductive system. The aim of this research was to see whether the ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum had any estrogenic activity in female wistar rats. Methods: In female wistar rats, the estrogenic effect was studied using a uterotropic assay, vaginal cytology and vaginal opening. In ovariectomized immature and mature female wistar rats, a 400 mg/kg body weight (b.w.) dose of ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum was given. Result: When compared to ovariectomized control rats, the uterine wet weight increased significantly. The estrogen-treated rats had only cornified epithelial cells, indicating the existence of oestrogen, as well as 100% vaginal opening. At 400 mg/kg b.w., the ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum demonstrated promising estrogenic activity, as evidenced by uterotropic assays, vaginal opening measurements and histopathological changes. As a result of this research, it’s possible to infer that the ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum play an important role in estrogenic activity in female rats.

scholarly journals Naloxone Breeding Effectiveness in Rat Suffering from Nitric Oxide-induced Polycystic Ovary Syndrome

2015 ◽  
Vol 6 (2) ◽  
pp. 67-72
Author(s):  
Manizheh Karami ◽  
Fatemeh Lakzaei ◽  
MohammadReza Jalali Nadoushan
Keyword(s):  
Nitric Oxide ◽  
Polycystic Ovary Syndrome ◽  
Wistar Rats ◽  
Polycystic Ovary ◽  
Treated Group ◽  
Female Rats ◽  
Control Group ◽  
Ovary Syndrome ◽  
Crown Rump Length ◽  
Female Wistar Rats

ABSTRACT Background and objective Polycystic ovary syndrome (PCOS) can be induced in Wistar rats by over production of nitric oxide (NO). This study evaluated the efficacy of naloxone on the breeding characteristics of rats suffering from nitric oxide induced PCOS. Materials and methods Twenty-four female Wistar rats(200–250 gm) were kept as virgin under standard conditions. They were divided into four groups (n = 6). One group of the animals received L-arginine (50 mg/kg) intraperitoneally (i.p.) for 9 days/once a day. Another group was administered naloxone hydrochloride (0.4 mg/kg, i.p.) prior to injection of L-arginine. The third group was injected solely naloxone. Control group received saline solution (1 ml/kg, i.p.). After the treatments, all female rats were coupled with the intact males. They were then separated by observation of vaginal plaques; it was considered as day 0 of pregnancy. Eventually, they were operated on days 18 to 19 of the gestation to collect the animals’ ovaries. The samples were studied for pathological evidence. The fetal number and weight along with the fetal crown-rump length (CRL) were measured. Results The ovaries obtained from the L-arginine treated group had large cysts with thickened granulosa cell layer in contrast to those of the control or naloxone treated rats (p < 0.0001). The number of fetus though showed a decrease in the L-arginine treated rats (3 ± 1), but the fetal weight or fetal CRL did not change (p > 0.05). Conclusion This study may clearly illustrate the polycystic characteristics in the L-arginine treated group. It may particularly display the breeding efficacy of naloxone in rats with PCOS. How to cite this article Karami M, Lakzaei F, Nadoushan MRJ. Naloxone Breeding Effectiveness in Rat Suffering from Nitric Oxide-induced Polycystic Ovary Syndrome. Int J Infertil Fetal Med 2015;6(2):67-72.

Histomorphometry and expression of Cdc47 and caspase-3 in hyperthyroid rat uteri and placentas during gestation and postpartum associated with fetal development

2007 ◽  
Vol 19 (3) ◽  
pp. 498 ◽  
Author(s):  
E. S. Freitas ◽  
E. D. Leite ◽  
C. A. Souza ◽  
N. M. Ocarino ◽  
E. Ferreira ◽  
...  
Keyword(s):  
Adult Female ◽  
Morphometric Analysis ◽  
Fetal Development ◽  
Wistar Rats ◽  
Caspase 3 ◽  
Female Rats ◽  
Control Female ◽  
Uterine Involution ◽  
Female Wistar Rats ◽  
Medium Diameter

In two different experiments, the effects of hyperthyroidism on the histomorphometry and expression of Cdc47 and caspase-3 were evaluated in the uteri and placentas during gestation and postpartum. Fetal development was also evaluated during gestation. In the first experiment, 36 adult female Wistar rats were divided into two groups of 18 animals each: (1) hyperthyroid; and (2) euthyroid (control). Female rats were mated and killed at 7, 14 and 19 days of gestation. Uteri and placentas were weighed and subjected to histomorphometric and immunohistochemical evaluation to determine the expression of Cdc47 and caspase-3. Ovaries were also evaluated for weight and subjected to morphometric analysis. Fetuses were quantified and weighed individually. In the second experiment, 12 adult female Wistar rats were divided into two groups of six animals each: (1) hyperthyroid; and (2) euthyroid (control). Female rats were mated and killed 2 days postpartum. Uteri were evaluated in the same way as for the first experiment. Hyperthyroidism increased ovulation and conception rates without disturbing the size and viability of the fetuses. In the pregnant uteri, hyperthyroidism did not change the thickness of the layers or the expression of Cdc47 and caspase-3. However, in the placentas, hyperthyroidism increased the medium diameter of trophoblast cells, as well as the thickness and the expression of Cdc47 of spongiotrophoblast cells, at 14 days of gestation. During uterine involution, hyperthyroidism significantly increased the expression of Cdc47 and reduced the expression of caspase-3 in the uterine layers. In conclusion, hyperthyroidism increased the conception rate because of an ovulation gain, induced significant placental changes during pregnancy and, in the uterus, increased Cdc47 expression and decreased caspase-3 expression after parturition.

NEW DATA CONCERNING THE ROLE PLAYED BY PROGESTERONE IN THE CONTROL OF FOLLICULAR GROWTH IN THE RAT

1974 ◽  
Vol 75 (3) ◽  
pp. 569-578 ◽  
Author(s):  
G. Buffler ◽  
S. Roser
Keyword(s):  
Wistar Rats ◽  
Venous Blood ◽  
Oestrous Cycle ◽  
Female Rats ◽  
Cycle Duration ◽  
Follicular Growth ◽  
The Third ◽  
Female Wistar Rats

ABSTRACT The mechanisms involved in the prolongation of the oestrous cycle following LH administration were studied in 4-day cyclic female Wistar rats. In females injected with LH on the morning of dioestrus I there was an increase in ovarian venous blood progesterone as compared with non-injected animals. In both LH-treated females, and those injected with progesterone on the morning of dioestrus I, a slowing up in follicular growth was observed from the afternoon of dioestrus I. The size of follicles greater than 400 urn present in LH or progesterone injected animals on the third day of cycle was similar to the size reached by the same range of follicles in non-injected animals on the second day of the cycle. Hence, the increase in endogenous ovarian progesterone elicited by LH was considered as the cause of the slowing up of follicular growth and therefore of the lengthening of the oestrous cycle duration in female rats injected with LH at the beginning of 4-day cycle.

scholarly journals Are skeletally mature female rats a suitable model to study osteoporosis?

2012 ◽  
Vol 56 (4) ◽  
pp. 259-264 ◽  
Author(s):  
Claudia Cardoso Netto ◽  
Vivian Cristine Correia Vieira ◽  
Lizanka Paola Figueiredo Marinheiro ◽  
Sherry Agellon ◽  
Hope Weiler ◽  
...  
Keyword(s):  
Bone Metabolism ◽  
Type I Collagen ◽  
Biomechanical Properties ◽  
Mature Female ◽  
Female Rats ◽  
Suitable Model ◽  
Type I ◽  
Age Related ◽  
Female Wistar Rats ◽  
Old Rats

OBJECTIVE: To analyze if female Wistar rats at 56 weeks of age are a suitable model to study osteoporosis. MATERIALS AND METHODS: Female rats with 6 and 36 weeks of age (n = 8 per group) were kept over a 20-week period and fed a diet for mature rodents complete in terms of Ca, phosphorous, and vitamin D. Excised femurs were measured for bone mass using dual-energy x-ray absorptiometry, morphometry, and biomechanical properties. The following serum mar-kers of bone metabolism were analyzed: parathyroid hormone (PTH), osteocalcin (OC), osteoprotegerin (OPG), receptor activator of nuclear factor Κappa B ligand (RANKL), C-terminal peptides of type I collagen (CTX-I), total calcium, and alkaline phosphatase (ALP) activity. RESULTS: Rats at 56 weeks of age showed important bone metabolism differences when compared with the younger group, such as, highest diaphysis energy to failure, lowest levels of OC, CTX-I, and ALP, and elevated PTH, even with adequate dietary Ca. CONCLUSION: Rats at 26-week-old rats may be too young to study age-related bone loss, whereas the 56-week-old rats may be good models to represent the early stages of age-related changes in bone metabolism.

Sex differences in spironolactone induction of rat intestinal and hepatic p-nitrophenol UDP-glucuronyltransferase

1990 ◽  
Vol 68 (10) ◽  
pp. 1385-1387 ◽  
Author(s):  
Viviana A. Catania ◽  
Marcelo G. Luquita ◽  
María C. Carrillo ◽  
Aldo D. Mottino
Keyword(s):  
Enzyme Activity ◽  
Sex Differences ◽  
Wistar Rats ◽  
Female Rats ◽  
Intestinal Tissue ◽  
Male And Female ◽  
Percent Increase ◽  
Female Wistar Rats ◽  
Related Response ◽  
Intestinal Enzyme

In the present study we analyzed the effect of spironolactone administration on hepatic and intestinal p-nitrophenol-UDP-glucuronyltransferase activity. We used microsomal preparations from male and female Wistar rats to establish whether or not this effect was sex dependent. Enzyme activity was measured in the presence of UDP-N-acetylglucosamine, a presumed physiological activator of the enzyme. Female but not male microsomes showed an increase in enzyme activity of both hepatic and intestinal tissue preparations in response to the inducer pretreatment. In addition, the inducer effect observed in female rats showed a tissue-related difference, since percent increase in the intestinal enzyme activity was greater than that in the liver (127 and 52%, respectively). These results suggest that factors regulating enzyme activity or mechanisms involved in the inducer effect of spironolactone could be different in the intestinal mucosa in comparison to the liver. A possible explanation of sex-related response to spironolactone administration was discussed.Key words: p-nitrophenol, UDP-glucuronyltransferase, spironolactone induction, sex differences.

An analysis of the course of infection of Moniliformis dubius (Acanthocephala) in rats

Parasitology ◽  
1972 ◽  
Vol 64 (3) ◽  
pp. 517-523 ◽  
Author(s):  
D. W. T. Crompton ◽  
D. E. Walters
Keyword(s):  
Wistar Rats ◽  
Female Rats ◽  
Male Rats ◽  
Male And Female ◽  
Oral Infections ◽  
The Third ◽  
Male And Female Rats ◽  
Female Wistar Rats ◽  
Average Recovery Rate ◽  
Marked Decline

An analysis of the course of infection of mixed oral infections of 12 cystacanths of Moniliformis dubius in 174 male and 179 female Wistar rats has been undertaken.There was a marked decline in the average recovery rate of worms of both sexes from hosts of both sexes during the course of the infection.Female worms from both male and female rats showed, on average, a greater power of survival than male worms from the third period (10–13 weeks) onwards.Male rats were found to retain, on average, a greater number of worms of both sexes than female rats.We wish to thank Miss Susan Arnold and Mr David Barnard for excellent technical help.

scholarly journals Tolerance to hypothermic and antinoceptive effects of Δ9-tetrahydrocannabinol (THC) vapor inhalation in rats

2017 ◽  
Author(s):  
Jacques D. Nguyen ◽  
Yanabel Grant ◽  
Tony M. Kerr ◽  
Arnold Gutierrez ◽  
Maury Cole ◽  
...  
Keyword(s):  
Propylene Glycol ◽  
Wistar Rats ◽  
Female Rats ◽  
Male Rats ◽  
Preclinical Studies ◽  
Male And Female ◽  
Temperature Changes ◽  
Male And Female Rats ◽  
Antinociceptive Effects ◽  
Female Wistar Rats

AbstractRationaleA reduced effect of a given dose of Δ9-tetrahydrocannabinol (THC) emerges with repeated exposure to the drug. This tolerance can vary depending on THC dose, exposure chronicity and the behavioral or physiological measure of interest. A novel THC inhalation system based on e-cigarette technology has been recently shown to produce the hypothermic and antinociceptive effects of THC in rats.ObjectiveTo determine if tolerance to these effects can be produced with repeated vapor inhalation.MethodsGroups of male and female Wistar rats were exposed to 30 minutes of inhalation of the propylene glycol (PG) vehicle or THC (200 mg/mL in PG) two or three times per day for four days. Rectal temperature changes and nociception were assessed after the first exposure on the first and fourth days of repeated inhalation.ResultsFemale, but not male, rats developed tolerance to the hypothermic and antinociceptive effects of THC after four days of twice-daily THC vapor inhalation. Thrice daily inhalation for four days resulted in tolerance in both male and female rats. The plasma THC levels reached after a 30 minute inhalation session did not differ between the male and female rats.ConclusionsRepeated daily THC inhalation induces tolerance in female and male rats, providing further validation of the vapor inhalation method for preclinical studies.AbbreviationsPG, propylene glycol; THC; Δ9tetrahydrocannabinol;

scholarly journals Kefir as a Prevention of Arsenic-mediated Toxicity in Uterine Female Rats

2021 ◽  
Vol 4 (4) ◽  
Author(s):  
Sabatina Windyaningrum ◽  
◽  
Tri Yudani Mardining Raras ◽  
Bambang Rahardjo ◽  
Rose Khasana Dewi
Keyword(s):  
Protective Effect ◽  
Arsenic Trioxide ◽  
Stromal Cells ◽  
Wistar Rats ◽  
Endometrial Thickness ◽  
Normal Diet ◽  
Female Rats ◽  
Tissue Preparation ◽  
Endometrial Stromal Cells ◽  
Female Wistar Rats

Background: kefir is a fermented milk product that demonstrates numerous health benefits including antioxidant and immunomodulatory. Aim: to study the protective effect kefir on the expression of estrogen receptor alpha (ERα) in endometrial stromal cells and endometrial thickness on female rats that were exposed to arsenic. Methods: twenty-five female Wistar rats (Rattus norvegicus) were divided into five groups (CRL, As, T1, T2, T3). Control group (given a normal diet), As group (given the normal diet and exposed to arsenic trioxide 2 mg/kgBW/day). The T1; T2; T3 were exposed to arsenic trioxide 2 mg/kgBW/day and treated with different doses of kefir (1.25; 2.5; and 5 mL/kgBW/day, respectively) for 35 days. The rats of group As treated with arsenic trioxide only and group CRL served as control with normal feed in water. Cytological samples were taken after 35 days of treatment and examined every day to see the rat oestrus phase, and the proestrus phase of the oestrous cycle was chosen for termination. Uterine tissue fixed in 10% neutral buffered formalin for tissue preparation. ERα expression in endometrial stromal cells was analized using immunohistochemistry method, endometrial thickness was observed using histopathological methods. Results: significant reduction of ERα expression in endometrial stromal cells and endometrial thickness in female rats exposed to arsenic were observed in groups on treated rats (p ≤ 0.000; 0.009, respectively). Conclusion: the administration of kefir in female Wistar rats exposed to arsenic had shown significantly differences on ERα expressions and endometrial thickness. The smallest dose of kefir (1.25 mL/kgBW/day) could increase ERα expression and endometrial thickness in female Wistar rats with arsenic exposure. Therefore kefir has protective effect related to female reproductive system.

scholarly journals Gestational and neurodevelopmental effects of black mustard seeds' (Brassica nigra) extract in wistar rats

1970 ◽  
Vol 8 (1) ◽  
pp. 1368-1378
Author(s):  
Bernard Ufuoma Enaibe ◽  
Tolulope Timothy Arogundade ◽  
Oluwaseun Adigun ◽  
Foyeke Munirat Adigun ◽  
Emmanuel Olusola Yawson ◽  
...  
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Treated Group ◽  
Brassica Nigra ◽  
Female Rats ◽  
Rat Pups ◽  
Group A ◽  
Black Mustard ◽  
Group B ◽  
Mustard Seeds

This study investigated the effect of the crude aqueous extract of Brassica nigra (Black Mustard Seeds) in gestation and on the prefrontal cortex of newborn Wistar rats at different doses following prenatal administration. Eighteen (18) adult female rats weighing an average of 180±10g were used. The female rats were split into 3 groups of six animals; Group A received distilled water throughout gestation, Group B received 200 mg/kg body weight of extract throughout gestation, and Group C received 100 mg/kg body weight of extract throughout gestation). Rat pups from the experimental groups were sacrificed on postnatal days 1, 7, 14, 21, 28, and 35 and subsequently prepared through routine histological and histochemical procedures. Brassica nigra was abortifacient at 200 mg/kg body weight and reduced litter size at 100 mg/kg body weight. No observed physical deformities in pups of treated groups. Comparative prefrontal microarchitecture revealed little to no alteration in the treated group. This study concludes that Brassica nigra (black mustard) is not totally innocuous and as such, should be moderately consumed or totally avoided in pregnancy.Keywords: Brassica nigra; Mustard seeds; Gestation; Neurodevelopment.

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