The activity of alcoholic extract of Garlic on the growth of Staphylococcus aureus with estimation of median lethal dose in lab. Mice
The study was intended to investigate the in vitro activity of alcoholic extract of Garlic on the inhibition of growth of Staph. aureus which was isolated from skin infections , and determine the median lethal dose ( LD 50 ) of the extract in lab. mice. The Garlic was extracted by ethyl alcohol 95% , the ratio of ethanolic extraction amounted 44% of the weight of dry substance. Graduated concentration were prepared from alcoholic extract of Garlic from 10-100 mg/ml. Their activities were checked up against Staph.aureus by agar diffusion method using ethylene glycol as control. The results showed that the sensitivity of the test bacteria was gradually increased with increasing the extract concentration , the concentration 10-30 mg/ml were rather low active in preventing the growing of Staph. aureus in culturing media , the concentrations 40-70 mg/ml were moderately active ,meanwhile the concentrations 80-100 mg/ml were highly active against the growing of Staph.aureus . The results also showed that the LD50 of the ethanolic extract of Garlic when it is orally administered to the lab mice by gradual concentrations was about 8000 mg/kg body weight . The toxic signs during 24 hrs after initial feeding with the extract were rapid breathing followed by dullness , then death