Uji Efektivitas Bioinsektisida Berbahan Aktif Beauveria bassiana (Balsamo) Vuillemin terhadap Kutudaun Macrosiphoniela sanborni pada Krisan

Kutudaun Macrosiphoniela sanborni merupakan salah satu hama penting yang menimbulkan kerugian yang cukup serius pada budidaya krisan. Untuk mengendalikannya petani biasa menggunakan pestisida kimia sintetis. Selain itu pengendalian hayati berpotensi dapat menekan hama. Beauveria bassiana merupakan agens pengendali hayati yang memiliki potensi besar untuk mengendalikan beberapa hama penting tanaman hias. Biorama 1, 2, dan 3 merupakan bioinsektisida berbahan aktif B. bassiana dengan kepadatan1010 konidia per g bahan pembawa dengan bahan pembawa yang berbeda (tepung jagung, sekam, dan talk) yang dibuat di Laboratorium Biokontrol, Balai Penelitian Tanaman Hias (Balithi). Bioinsektisida tersebut terbukti efektif mengendalikan trips pada tanaman krisan. Penelitian bertujuan mengetahui efektivitas bioinsektisida Biorama 1, 2, dan 3 dalam mengendalikan kutudaun krisan. Penelitian dilaksanakan di Rumah Plastik Balithi Segunung sejak bulan Juli hingga November 2008. Bahan yang digunakan dalam penelitian ini ialah Dendranthema grandiflora kultivar Sakuntala. Perlakuan yang diuji yaitu Biorama 1 (1010 konidia/g tepung jagung), Biorama 2 (1010 konidia/g arang sekam), dan Biorama 3 (1010 konidia/g talk) masing-masing dengan konsentrasi aplikasi 5 g/l, suspensi B. bassiana dengan kepadatan 1010 konidia/ml, Natural BVR adalah bioinsektisida komersial berbahan aktif B. bassiana (1010 konidia/g bahan pembawa) dengan konsentrasi aplikasi 5 g/l sebagai kontrol positif dan air sebagai kontrol negatif. Penelitian disusun menggunakan rancangan acak kelompok terdiri atas enam perlakuan dan lima ulangan. Hasil penelitian menunjukkan bahwa bioinsektisida Biorama 1, 2, dan 3 efektif mengendalikan kutudaun M. sanborni dan efektivitasnya tidak berbeda nyata dibandingkan dengan Natural BVR dan suspensi Beauveria. Perlakuan tersebut mampu menekan serangan M. sanborni, masing-masing dengan 68,58, 60,59, dan 54,37 % secara berurutan. Bioinsektisida memberikan pengaruh nyata terhadap mortalitas kutudaun. Biorama 1 dan 2 menunjukkan keefektifan yang paling tinggi (22,30 dan 24,20%) dalam menekan kerusakan bunga dibandingkan dengan perlakuan lain dan kontrol. Implikasi dari penelitian ini ialah Biorama memiliki potensi yang besar diaplikasikan sebagai pestisida hayati ramah lingkungan pada budidaya krisan. Macrosiphoniela sanborni is one of the important pests causing serious losses on Chrysanthemum. Beauveria bassiana is a biological control agents, which has great potential to control several important pests on ornamental plants. Biorama 1, 2, and 3 are bioinsecticides containing B. bassiana as an active ingredient and made in Biological Control Laboratory of Indonesian Ornamental Crops Research Institute (IOCRI). The bioinsecticides were successfully tested and gave significant effect on controlling Chrysanthemum thrips. The study was aimed to determine the effectiveness of Biorama 1, 2, and 3 on controlling Chrysanthemum aphids. The experiment was conducted at the Plastichouse of IOCRI from July to November 2008. Plant material used in this study was Dendranthema grandiflora cv. Sakuntala. The treatments tested were Biorama 1 (1010 conidia/g corn powder), Biorama 2 (1010 conidia/g activated-carchoal), and Biorama 3 (1010 conidia/g talk) respectively with a concentration of 5 g/l, B. bassiana suspension with a density of 1010 conidia/ml, Natural BVR is a bioinsecticide with active ingredient B. bassiana, which has been commercialized as positive control in concentration 5 g/l and water as negative control. The experiment was arranged by a randomized complete block design consisted of six treatments and five replications. The results showed that Biorama 1, 2, and 3 were effective to control M. sanborni and the results did not give significant difference compared to Natural BVR and Beauveria suspension. They reduced Chrysanthemum aphids up to 68.58, 60.59, and 54,37% respectively. Bioinsecticide provide real impact on the mortality of aphids. The Biorama 1 and 2 indicated the highest effectiveness (22.3 and 24.2% respectively) on lowering to flower damage compared to other treatments and control. This results indicated that Biorama have high potential applied as an environmental friendly biopesticide on Chrysanthemum cultivation.

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Histopathological Profile of Therapeutic Doses of Mango Mistletoe Methanolic Extract (MMME) in Cardiac of Hypertensive Rats (DOCA-Salt)

JSMARTech ◽

10.21776/ub.jsmartech.2021.002.02.48 ◽

2021 ◽

Vol 2 (2) ◽

pp. 048-054

Author(s):

Annisatul Hakimah ◽

◽

Nour Sjakoer ◽

Nurul Mubarakati ◽

◽

...

Keyword(s):

Methanolic Extract ◽

Muscle Cells ◽

Positive Control ◽

Negative Control ◽

P Value ◽

Hypertensive Rats ◽

Control Groups ◽

Significant Difference ◽

Heart Muscle Cells ◽

And Control

This study determined the effect of Mango mistletoe Methanolic Extract (MMME) on the cardiac's histopathological profile of hypertensive rats (DOCA-Salt) treated. The MMME was treated on fifty rats for 14 days, divided into five treatments: negative control, positive control, doses 50, 100, and 200 mg/kg BW with five replications. One-way ANOVA analysis was used, namely JAMOVI with version 1.1.9.0, and for cell calculation, diameter using the IMAGE J application. Results showed that there were no significant differences in the histopathological profile of the cardiac in hypertensive rats (DOCA-Salt) treated with MMME for 14 days on the diameter of the heart muscle cells between the control (+) and control groups (-), treatments 1, 2, and 3. This is evidenced by the analysis of p-value> 0.05, namely 0.187 millimeters. Therefore, we concluded that MMME does not affect the diameter of heart/cardiac organ muscle cells. However, there was a significant difference in the amount of necrosis in the cardiac of hypertensive rats between control (+) and control (-) groups, treatments 1, 2, and 3. Based on the results, MMME reduces the number of necrosis in the heart/cardiac organ.

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Peningkatan Pertumbuhan Tanaman Cabai dan Pengendalian Busuk Phytophthora melalui Biopriming Benih dengan Rizobakteri Asal Pertanaman Cabai Jawa Timur

Jurnal Hortikultura Indonesia ◽

10.29244/jhi.8.3.171-182 ◽

2017 ◽

Vol 8 (3) ◽

pp. 171 ◽

Cited By ~ 2

Author(s):

Aulia Zakia ◽

Satriyas Ilyas ◽

Candra Budiman ◽

Syamsuddin , ◽

Dyah Manohara

Keyword(s):

Plant Growth ◽

Seed Treatment ◽

Block Design ◽

Phytophthora Capsici ◽

Positive Control ◽

Negative Control ◽

Phytophthora Blight ◽

Blight Disease ◽

And Control ◽

East West

ABSTRACT The objectives of this study was to evaluate biopriming of chili seed with rhizobacteria to improve plant growth and control Phytophthora blight disease in a greenhouse. This experiment used three isolates of rhizobacteria, i.e. E1, E3C2 and F2B1, and isolate Phytophthora capsici (Cb6) isolated from the production center of chili in East Jawa. Laris variety from PT. East West was used in this experiment. This experiment used randomized block design with one factor, i.e. 11 levels of seed treatment (E1 rhizobacteria, E3C2 rhizobacteria, F2B1 rhizobacteria, E1+E3C2 rhizobacteria, E1+F2B1 rhizobacteria, E1+E3C2+F2B1 rhizobacteria, seed soaking in water, without soaking, metalaxyl, positive control and negative control). The result showed that seed treatment with combination of E1+F2B1 isolates when grown in nursery, significantly increased the height and number of leaves in chilli. Besides, seed treatment with F2B1 isolate and combination of E1+F2B1 isolates after transplanting were capable to improve plant growth and control Phytophthora blight disease in greenhouse.Keywords: greenhouse, isolate rhizobacteria, Phytophthora capsici ABSTRAK Tujuan penelitian ini ialah mengevaluasi perlakuan biopriming benih cabai dengan rizobakteri dalam meningkatkan pertumbuhan bibit dan mengendalikan kejadian busuk Phytophthora di rumah kaca. Perlakuan biopriming benih dengan rizobakteri menggunakan tiga isolat rizobakteri E1, E3C2 dan F2B1 dan isolat Phytophthora capsici Cb6 hasil eksplorasi pertanaman cabai Jawa Timur. Benih yang digunakan dalam percobaan merupakan benih varietas Laris produksi PT. East West. Percobaan menggunakan rancangan acak kelompok satu faktor, masing-masing perlakuan diulang empat kali, dengan 11 taraf perlakuan, antara lain R0+ (kontrol positif, benih direndam dalam PDB tanpa perlakuan rizobakteri dengan inokulasi P. capsici), R0- (kontrol negatif, benih direndam dalam PDB tanpa perlakuan rizobakteri dan tanpa inokulasi P. capsici), R1 (perlakuan benih dengan isolat E1), R2 (isolat E3C2), R3 (isolat F2B1), R4 (kombinasi isolat E1+E3C2), R5 (kombinasi isolat E1+F2B1), R6 (kombinasi isolat E1+E3C2+F2B1), R0RA (benih direndam dalam air 24 jam), R0TR (benih tanpa rendam), R0M (benih direndam dalam metalaksil). Tanah inokulum P. capsici diberikan 28 hari setelah pindah-tanam di sekitar pangkal batang tanaman cabai di bawah permukaan tanah. Hasil percobaan menunjukkan, perlakuan dengan kombinasi isolat E1+F2B1 saat persemaian di rumah kaca nyata meningkatkan tinggi dan jumlah daun tanaman cabai. Perlakuan benih dengan isolat F2B1 maupun kombinasi isolat E1+F2B1 setelah pindah-tanam di rumah kaca memiliki kemampuan meningkatkan pertumbuhan tanaman serta mengendalikan penyakit busuk Phytophthora. Kata kunci: isolat rizobakteri, Phytophthora capsici, rumah kaca

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Pengaruh pemberian klorofilin berbagai dosis terhadap indeks fagositosis makrofag dan kadar nitric oxide mencit BALB/c yang diinfeksi dengan Salmonella typhimurium

JURNAL GIZI INDONESIA ◽

10.14710/jgi.2.2.78-82 ◽

2014 ◽

Vol 2 (2) ◽

pp. 77-81

Author(s):

Puspito Arum ◽

Lisyani B Suromo ◽

Niken Puruhita

Keyword(s):

Immune Responses ◽

Positive Control ◽

Negative Control ◽

Group Design ◽

Macrophage Phagocytosis ◽

Significant Difference ◽

Post Test ◽

Phagocytosis Index ◽

And Control ◽

One Way Anova

Background: Immune responses to eliminate Salmonella infection are by activating macrophage and by producing NO. Chlorophyllin is a chlorophyll derivate that has immunomodulator properties. Objective: The aim of this study was to prove effect of chlorophyllin in macrophage phagocytosis index and NO level. Methods: A post test only controlled group design was conducted in 5 groups Balb/c mice (negative control, positive control, dosage 100 µg/200 g BW, dosage 200 µg/200 g BW and dosage 380 µg/200 g BW). Macrophage phagocytosis index was measured by counting cells that phagocyte latexs particles. NO level was measured by Griess method. Macrophage phagocytosis index difference was analyzed by one way anova and NO level deference was analyzed by Kruskall-Wallis test (α 0,05).Results: Means of macrophage phagocytosis index were 0,7(±0,80), 1,8(±0,80), 2(±0,22), 2,5(±0,43) and 3,2(±0,68) respectively in negative control, positive control, chlorophyllin dosage 100 µg/g BW/day, 200 µg/g BW/day and 380 µg/g BW/day. There was a significant difference of macrophage phagocytosis index between group (p 0,000). Mean of NO level were 0,4 µM(±0,10), 0,6 µM(±0,60), 0,8 µM(±0,64), 0,6 µM(±0,67) and 0,4 µM(±0,26) respectively in negative control, positive control, chlorophyllin dosage 100 µg/g BW/day, 200 µg/g BW/day and 380 µg/g BW/day. There was no difference of NO level between group (p 0,813).Conclusion: There was a significant difference of macrophage phagocytosis index between chlorophyllin administered group and control. The higher chlorophyllin dosage, the higher macrophage phagocytosis index. Therewas no difference of NO level between chlorophyllin administered group and control.

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Evaluation of in vivo anti-malarial potential of omidun obtained from fermented maize in Ibadan, Nigeria

Malaria Journal ◽

10.1186/s12936-020-03486-0 ◽

2020 ◽

Vol 19 (1) ◽

Author(s):

Favour O. Omeiza ◽

George O. Ademowo ◽

Funmilola A. Ayeni

Keyword(s):

Scientific Evidence ◽

Positive Control ◽

Negative Control ◽

Control Treatment ◽

Malaria Therapy ◽

Significant Difference ◽

And Control ◽

Final Group ◽

Ibadan Nigeria

Abstract Background The menace of resistance to anti-malarial drugs is a great challenge to malaria control, necessitating the search for new anti-malarial agents. This search has led to the exploration of natural products for efficacy in malaria therapy. Omidun is the supernatant of fermenting maize (ogi) slurry that has been widely investigated and reported to possess several health benefits and it is used traditionally as solvent for preparing anti-malarial herbs. However, there is no information on the anti-malarial activity of omidun itself. This study was conducted to investigate the prophylactic, curative and suppressive anti-malarial potential of omidun. Methods Experimental mice in the curative group were infected with 1 × 106 cells of Plasmodium berghei strain ANKA and treated with either 0.2 ml of omidun containing 3 × 109 cfu/ml of viable lactic acid bacteria or 0.2 ml of 5 mg/kg of chloroquine (positive control) or 0.2 ml of saline (negative control) for 4 days from day 3 post infection. The prophylactic group of mice were pre-treated with either omidun, chloroquine or saline for 4 days before infection with P. berghei, while the suppressive group was treated with omidun or chloroquine or saline and infected with P. berghei simultaneously. A group of mice were uninfected but treated (with omidun and control samples), while a final group was uninfected and untreated (controls). Parasitaemia and histopathology analysis were done in all groups. Results The curative and suppressive groups showed a significant difference between the omidun-treated mice (100% parasitaemia reduction) and the untreated mice (54.5% parasitaemia increase). There was no significance difference between the omidun treatment and chloroquine (positive control) treatment in suppressive group as both treatment had 100% parasitaemia reduction. The omidun prophylactic treatment however did not show any parasitaemia suppression, but a significant difference was observed between the omidun treatment (85% increase) and the chloroquine (positive control) treatment (100% reduction) in the group. Omidun treatment is non-toxic to the kidney. Conclusion This study provides scientific evidence supporting omidun usage in the treatment of malaria. Consequently, further work may yield the specific component of omidun responsible for the anti-malarial activity.

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THE EFFECT OF Beauveria bassiana APPLICATION TO CONTROL MAIN PESTS OF STRING BEANS (Vigna sinensis) ON PLANT DAMAGE AND YIELD

Agrivet ◽

10.31315/agrivet.v25i1.4182 ◽

2019 ◽

Vol 25 (1) ◽

pp. 59

Author(s):

Hanafi Nursahid ◽

Rukmowati Brotodjojo ◽

Oktavia Sarhesti Padmini

Keyword(s):

Beauveria Bassiana ◽

Pest Control ◽

Block Design ◽

Positive Control ◽

Negative Control ◽

Vigna Sinensis ◽

Leaf Beetles ◽

Plant Damage ◽

Application Frequency ◽

Single Factor Experiment

String beans productivity decreases every year. One of the causes is pest attacks. Pest control using Beauveria bassiana is an enviromentally friendly pest control. The aim of this study was to determine of the effect B. bassiana’s application to control string beans main pests, determine the best B. bassiana application frequency to control pest attacks and determine the correlation between main pests population and plant damage and yield of string beans. This study was conducted in Krebet, Sendangsari, Pajangan, Bantul, Daerah Istimewa Yogyakarta in Januari – April 2019. This study used a single factor experiment arranged in a Randomized Completely Block Design (RCBD). This study used the following treatments: once application of B. bassiana at 5 weeks after planting (wap), two times application of B. bassiana at 3 wap (week after planting) and 7 wap, three times application of B. bassiana at 3 wap, 5 wap and 7 wap, four times application of B. bassiana at 3 wap, 4 wap, 6 wap and 7 wap, five times application of B. bassiana at 3 wap, 4 wap, 5 wap, 6 wap and 7 wap, negative control (without B. bassiana application) and positive control (synthetic insectiside profenofos application). The results of the study showed that B. bassiana could control Aphis craccivora. The best B. bassiana application frequency was 5 times at 3 wap, 4 wap, 5 wap, 6 wap and 7 wap. Leaves damage increased as the population of leaf beetles increased. Yield of string beans decreased as the population of A. craccivora and leaf beetles increased as well as the leaves damage increased.Keyword: main pests, Beauveria bassiana, damage, string beans, yield

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PEMANFAATAN BEBERAPA ISOLAT Trichoderma harzianum SEBAGAI AGENSIA PENGENDALI HAYATI PENYAKIT LAYU FUSARIUM PADA BAWANG MERAH IN PLANTA

EUGENIA ◽

10.35791/eug.17.2.2011.4105 ◽

2011 ◽

Vol 17 (2) ◽

Cited By ~ 3

Author(s):

Aenul Latifah ◽

. Kustantinah ◽

Loekas Soesanto

Keyword(s):

Fusarium Oxysporum ◽

Fusarium Wilt ◽

Trichoderma Harzianum ◽

Block Design ◽

Positive Control ◽

Negative Control ◽

In Planta ◽

Randomized Block Design ◽

Significant Difference ◽

Potato Isolate

ABSTRACT The green house research aimed to know the best isolate of T. harzianum in controlling Fusarium wilt , shallot production, and the effect of T. harzianum application time in suppressing the disease. Randomized block design was used with twelve treatments. Each treatment was replicated three times. The treatments were negative control, positive control as wel as extract of ginger isolate, banana isolate, shallot isolate, potato isolate, and ginseng isolates. The treatments were applied a week before or after inoculation of the pathogen. The result showed that the antagonist extract of ginger isolate applied a week before inoculation decreased the disease intensity by 43.854%. The antagonist extract application applied before or after inoculation were not significant difference . The antagonist of potato isolate extract was the best in increasing number of plants per clump and tuber fresh weight resulting 9.7503 and 13.133 g, respectively. The best extract in increasing root growth was shallot extract. The ginger isolate extract increased root length by 17.672%. Keywords: Trichoderma harzianum isolates, biological control, Fusarium wilt, shallot ABSTRAK Penelitian rumah kasa ini bertujuan untuk mengetahui isolat T. harzianum yang paling baik dalam mengendalikan penyakit moler dan meningkatkan hasil bawang merah, serta pengaruh waktu pemberian T. harzianum dalam menekan penyakit moler. Rancangan yang digunakan adalah Rancangan Acak Kelompok (RAK), yang terdiri atas 12 perlakuan dengan 3 ulangan. Perlakuan yang dicoba adalah kontrol negatif (tanpa perlakuan T. harzianum dan Fusarium oxysporum) dan kontrol positif (hanya dengan inokulasi F. oxysporum), serta ekstrak isolat jahe, pisang, bawang merah, kentang, dan ginseng yang diberikan 1 minggu sebelum atau sesudah inokulasi. Hasil penelitian menunjukkan bahwa ekstrak T. harzianum isolat jahe yang disiram sebelum inokulasi Fusarium oxysporum, mampu menurunkan intensitas penyakit sebesar 43,854%. Belum terlihat jelas pengaruh nyata pemberian ekstrak antagonis sebelum maupun sesudah inokulasi patogen. Ekstrak T. harzianum isolat kentang adalah isolat terbaik dalam meningkatkan jumlah anakan per rumpun dan bobot basah umbi, yaitu masing-masing 9,7503 dan 13,133 g, sedangkan ekstrak terbaik dalam pertumbuhan akar yaitu isolat bawang merah yang meningkatkan jumlah akar sebesar 57,452% dan ekstrak isolat jahe menunjukkan pengaruh terbaik meningkatkan panjang akar sebesar 17,672%. Eugenia Volume 17 No. 2 Agustus 2011 Kata Kunci: Isolat Trichoderma harzianum, pengendalian hayati, penyakit layu Fusarium, bawang merah

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Potensi Beberapa Fungisida Nabati dalam Mengendalikan Karat Putih (Puccinia horiana Henn.) dan Perbaikan Mutu Krisan

Jurnal Hortikultura ◽

10.21082/jhort.v22n4.2012.p385-391 ◽

2016 ◽

Vol 22 (4) ◽

pp. 385

Author(s):

Evi Silvia Yusuf ◽

Waqiah Nuryani ◽

Ika Djatnika ◽

Hanudin ◽

Suhardi ◽

...

Keyword(s):

Environmental Sustainability ◽

Block Design ◽

Positive Control ◽

Negative Control ◽

Production Costs ◽

Dendranthema Grandiflora ◽

Rust Disease ◽

Promising Alternative ◽

White Rust ◽

Puccinia Horiana

Puccinia horiana Henn. merupakan patogen penting penyebab penyakit karat putih yang menimbulkan kerugian signifikan dalam budidaya krisan, baik bunga potong atau tanaman pot. Aplikasi fungisida sintetik yang sering diandalkan oleh petani dan pengusaha tidak hanya memerlukan biaya yang lebih mahal, namun juga berdampak pada kerusakan lingkungan. Oleh karena itu pemanfaatan fungisida nabati yang lebih murah dan ramah terhadap lingkungan dapat menjadi alternatif pemecahannya. Beberapa fungisida nabati seperti Cees EC, Neem-plus, Cekam EC, dan Sitron-E berbahan aktif minyak atsiri cengkih, nimba, kayu manis, serai wangi, dan asam salisilat telah diproduksi dan dikomersialisasikan oleh Balai Penelitian Tanaman Rempah dan Obat. Aplikasi fungisida tersebut diduga berpengaruh positif dalam menekan penyakit karat putih. Penelitian bertujuan untuk memperoleh informasi keefektifan empat produk fungisida nabati tersebut dalam mengendalikan penyakit karat putih dan meningkatkan kualitas pertumbuhan krisan. Penelitian dilakukan di Rumah Plastik di Poncokusumo, Malang, Jawa Timur sejak Bulan Januari hingga Desember 2010. Bahan tanaman yang digunakan ialah Dendranthema grandiflora cv. Swarna Kencana. Perlakuan yang diuji ialah 3 ml/l untuk Cees EC, Neem-plus, Cekam EC, dan Sitron-E, serta 1,5 ml/l Amistartop 35 EC sebagai kontrol positif dan air sebagai kontrol negatif. Percobaan disusun menggunakan rancangan acak kelompok dengan enam perlakuan dan empat ulangan. Hasil penelitian menunjukkan bahwa semua fungisida nabati yang diuji efektif mengendalikan penyakit karat pada krisan. Perlakuan tersebut menurut uji statistik memiliki kemampuan yang sebanding dengan Amistartop. Penurunan intensitas karat putih oleh perlakuan Cees EC, Neem-plus, Cekam EC, Sitron-E, dan Amistartop berturut-turut sebesar 49; 37,74; 32,43; 29,78; dan 48,33%. Aplikasi Cees EC, Neem-plus, Cekam EC, dan Sitron-E tidak berpengaruh nyata terhadap tinggi tanaman, diameter batang, diameter bunga, dan vaselife bunga. Aplikasi hasil penelitian ini dapat memberi manfaat untuk petani dan pengusaha dalam menurunkan biaya produksi serta meningkatkan pendapatan petani dan pengusaha karena harga keempat biofungisida murah dan tanpa dampak negatif terhadap kelestarian lingkungan. Controlling white rust disease (Puccinia horiana Henn.) on chrysanthemum with some biofungicides P. horiana Henn. is important pathogen causing white rust disease that may lead to a significant lost in chrysanthemum cultivation (both for cut flower and pot plant). Synthetic fungicide commonly applied by farmers are causing not only high production costs, but also endangering the environment. Confronting to this situation, the use of biofungicide that are considered cheaper and more environmental friendly has become a relevant and promising alternative. Several biofungicides such as Cees EC, Neem-plus, Cekam EC, and Sitron-E with active ingredient of clove oil, neem, cinnamon, citronella, and salicylic acid have been commersialized by the Indonesian Medical and Spice Crops Research Institute. In this study those biofungicides were hypothesized to have great potential in control the white rust disease. The main objective of this study was to obtain information regarding the efficacy of four biofungicides in controlling white rust disease on chrysanthemum. The experiment was conducted at Plastichouse in Poncokusumo, Malang, East Java from January to December 2010 by using Dendranthema grandiflora cv. Swarna Kencana as planting materials. The treatments were consisted of 3 ml/l application of Cees EC, Neem-plus, Cekam EC, and Sitron-E 1.5 ml/l application of difenokonazol + azoxistrobin (Amistartop 35 EC) as a positive control and water as negative control. The experiment was set up using a randomized block design with six treatments and four replications. The results showed that all tested biofungicides were quite effective in controlling white rust disease on chrysanthemum and had similar effectiveness in reducing disease intensity compared to Amistartop. Cees EC, Neem-plus, Cekam EC, Sitron-E, and Amistartop had been able to reduce the white rust disease intensity by 49; 37.74; 32.43; 29.78; and 48.33% respectively. In the meantime, those biofungicides did not show significant effect on plant height, stem diameter, flower diameter, and flower vaselife. The use of biofungicides seems potentially promising to increase farmers income because the price of biofungicides were cheap and maintain environmental sustainability.

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Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v9i2.2015.71-77 ◽

2017 ◽

Vol 9 (2) ◽

pp. 71

Author(s):

Nurhasanah Nurhasanah ◽

Fauzia Andrini ◽

Yulis Hamidy

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Allium Sativum ◽

Fungicidal Activity ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) PADA MENCIT PUTIH JANTAN (Mus musculus) DENGAN INDUKSI NYERI ASAM ASETAT

Jurnal Ilmiah Manuntung ◽

10.51352/jim.v2i2.59 ◽

2017 ◽

Vol 2 (2) ◽

pp. 147

Author(s):

Triswanto Sentat ◽

Susiyanto Pangestu

Keyword(s):

Analgesic Effect ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Control Treatment ◽

Treatment Groups ◽

Analgesic Effects ◽

Significant Difference ◽

Analgesic Dose ◽

Muntingia Calabura

Kersen leaf (Muntingia calabura L.) contains tannins, flavonoids and polyphenol compounds allegedly have analgesic effect. The objective was to determine the analgesic effect of ethanol extract of kersen leaves and to determine the most effective analgesic dose. This study was an experimental research. Leaves were extracted with ethanol 70% and the analgesic effect test was divided into 5 groups: negative control treatment (distilled water), positive control (mefenamic acid 2.6mg/kg), kersen leaf ethanol extract first dose (100mg/kg), second dose (200mg/kg) and tthird dose (400mg/kg). Giving treatments by oral, after 30 minutes, the mices were given a pain inductor with 0.5% acetic acid by intra peritonial administration. Analgesic power was calculated by counting the number of writhing in mice for 1 hour. The results showed that the ethanol extract of cherry leaf has analgesic effect. From the calculation of the first dose analgesic power (42.9%), second dose (59.4%) and the third dose 69.9%. Statistical test results kruskal wallis value of p=0.011 (p<0.05) showed a significant difference between all analgesic treatment groups. The conclusion of this study is all of the ethanol extract had analgesic effects on male white mice, whereas a dose of 400mg/kg is the most effective analgesic dose.

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Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00548 ◽

2021 ◽

pp. 3143-3149

Author(s):

Endang Sri Purwanti Ningsih ◽

Noorlaila Noorlaila ◽

Ikhwan Rizki Muhammad ◽

Windy Yuliana Budianto

Keyword(s):

Wound Healing ◽

Wistar Rats ◽

Leaf Extract ◽

Wound Care ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Significant Difference ◽

Male Wistar Rats ◽

Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

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