scholarly journals Studi Akumulasi Pigmen β-Cryptoxanthin untuk Membentuk Warna Jingga Buah Jeruk di Daerah Tropika

2019 ◽  
Vol 9 (2) ◽  
pp. 73-83
Author(s):  
Inanpi Hidayati Sumiasih ◽  
Taruna Shafa Arzam ◽  
Roedhy Poerwanto ◽  
Darda Efendi ◽  
Andria Agusta ◽  
...  
Keyword(s):  
Chlorophyll Content ◽  
Room Temperature ◽  
Color Index ◽  
Transformation Process ◽  
Total Chlorophyll ◽  
Citrus Fruits ◽  
Citrus Peel ◽  
Total Chlorophyll Content ◽  
Citrus Varieties ◽  
Orange Color

ABSTRACTDegreening is a transformation process on peel which enables it to change color from green to orange on citrus fruits. The orange color of the peel comes from the mixture of carotenoid pigments, such as β-cryptoxanthin and β-citraurin. The pigments contributed in the formation of β-citraurin are β-cryptoxanthin and zeaxanthin. The objectives of this study were (1) to obtain proper degreening temperature in the orange color formation of several citrus varieties, and (2) to identify and determine pigments of β-cryptoxanthin pigment and total chlorophyll content in citrus peel after degreening. This study was conducted at PKHT IPB and LIPI Cibinong from July 2013 to December 2013, and from February 2016 to May 2017. About 100 ppm of ethylene gas was injected into a citrus-containing box using 5 ml syringe, then the box was placed in cool storage at 15 0C, 20 0C and room temperature, for 72 hours. The results showed that the best colors of Keprok Selayar and Keprok Tejakula were obtained by the degreening at 15 0C, in Siam Kintamani it was obtained by degreening at 20 0C. Degreening significantly reduced the total chlorophyll content, and increased β-cryptoxanthin content. The content of β-cryptoxanthin after degreening was 3 folds higher on highland Citrus reticulata than lowland citrus.Keywords: citrus color index, chlorophill, degreening, ethylene, tropical citrusABSTRAKDegreening adalah proses perombakan warna hijau pada kulit jeruk diikuti dengan proses pembentukan warna jingga. Warna jingga adalah campuran antara β-cryptoxanthin dengan β-citraurin. Pigmen yang berkontribusi dalam pembentukan β-citraurin adalah β-cryptoxanthin dan zeaxanthin. Tujuan penelitian ini ialah (1) Mendapatkan suhu degreening yang tepat dalam pembentukan warna jingga pada beberapa varietas jeruk, (2) Identifikasi dan penentuan kadar pigmen β-cryptoxanthin dan kandungan total klorofil pada kulit jeruk setelah degreening. Penelitian ini dilakukan di PKHT IPB dan LIPI Cibinong pada bulan Juli 2013 sampai Desember 2013, dan bulan Februari 2016 sampai Mei 2017. Degreening dilakukan dengan menginjeksikan gas etilen konsentrasi 100 ppm ke dalam wadah tertutup yang berisi jeruk menggunakan syringe 5 ml, kemudian disimpan pada suhu 15 0C, 20 0C dan suhu ruang, selama 72 jam. Hasil penelitian menunjukkan bahwa warna terbaik jeruk Keprok Selayar dan Tejakula diperoleh dengan degreening pada suhu 15 0C, Siam Kintamani diperoleh dengan degreening pada suhu 20 0C. Degreening dapat menurunkan kandungan total klorofil secara tajam, dan terbukti meningkatkan kandungan pigmen β-cryptoxanthin. Kandungan pigmen β-cryptoxanthin setelah degreening 3 kali lebih tinggi pada jeruk keprok dataran tinggi dibandingkan dengan dataran rendah.Kata kunci: citrus color index, degreening, etilen, jeruk tropika, klorofil

scholarly journals The Analysis of β-cryptoxanthin and Zeaxanthin using HPLC in the Accumulation of Orange Color on Lowland Citrus

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Inanpi Hidayati Sumiasih ◽  
Roedhy Poerwanto ◽  
Darda Efendi ◽  
Andria Agusta ◽  
Sri Yuliani
Keyword(s):  
Color Index ◽  
Total Dissolved Solids ◽  
Citrus Reticulata ◽  
Total Chlorophyll ◽  
Exposure Control ◽  
Citrus Peel ◽  
Total Chlorophyll Content ◽  
Peel Color ◽  
Bright Orange ◽  
Orange Color

Citrus peel color is one of the main quality attributes which was caused by the accumulation of carotenoids and its derivatives, especially β-citraurine. It makes citrus peel color looks attractive (orange). The orange color is a mixture of β-cryptoxanthin with β-citraurin. The objectives of this study were (1) to observe the effect of precooling and duration of proper ethylene exposure in the formation of orange color on citrus peel, (2) to identify and determine the β-cryptoxanthin content and total chlorophyll on citrus peel. Citrus was from Tuban, East Java while the study was conducted at PKHT IPB and LIPI. Precooling and without precooling treatment prior to injection of 100 ppm of ethylene exposed at 15 °C, duration of exposure control (0), 24, and 48 hours. The results show that the best color of the Citrus Color Index (CCI) is the precooling treatment and the duration of ethylene exposure for 24 hours, which can reduce total chlorophyll content about 8 times and proved to increase β-cryptoxanthin pigment content five times in accelerating the formation of orange citrus reticulata peel color to bright orange. Degreening has no significant effect on total dissolved solids and the firmness level of citrus fruits.Keyword: β-cryptoxhantin; citrus; chlorophyll; degreening; ethylene zeaxanthin.

scholarly journals Improving the Keeping Quality and Vase Life of Cut Alstroemeria Flowers by Pre and Post-harvest Salicylic Acid Treatments

2013 ◽  
Vol 5 (3) ◽  
pp. 364-370 ◽  
Author(s):  
Elnaz SOLEIMANY-FARD ◽  
Khodayar HEMMATI ◽  
Ahmad KHALIGHI
Keyword(s):  
Salicylic Acid ◽  
Water Balance ◽  
Chlorophyll Content ◽  
Water Loss ◽  
Vase Life ◽  
Total Chlorophyll ◽  
Cut Flowers ◽  
Fresh Weight ◽  
Total Chlorophyll Content ◽  
Keeping Quality

Keeping quality and length of vase life are important factors for evaluation of cut flowers quality, for both domestic and export markets. Studding the effect of pre- and post-harvest salicylic acid applications on keeping quality and vase life of cut alstroemeria flowers during vase period is the approach taken. Aqueous solutions of salicylic acid at 0.0 (with distilled water), 1, 2 and 3 mM were sprayed to run-off (approximately 500 mL per plant), about two weeks before flowers harvest. The cut flowers were harvested in the early morning and both of cut flowers treated (sprayed) and untreated were kept in vase solutions containing salicylic acid at 0.0 (with distilled water), 1, 2 and 3 mM. Sucrose at 4% was added to all treatments as a base solution. The changes in relative fresh weight, water uptake, water loss, water balance, total chlorophyll content and vase life were estimated during vase period. The results showed that the relative fresh weight, water uptake, water balance, total chlorophyll content and vase life decreased significantly while the water loss increased significantly during experiment for all treatments. A significant difference between salicylic acid and control treatments in all measured parameters is observed. During vase period, the salicylic acid treatments maintained significantly a more favourable relative fresh weight, water uptake, water balance, total chlorophyll content and supressed significantly water loss, as compared to control treatment. Also, the results showed that the using salicylic acid increased significantly the vase life cut alstroemeria flowers, over control. The highest values of measured parameters were found when plants were treated by pre + post-harvest application of salicylic acid at 3 mM. The result revealed that the quality attributes and vase life of cut alstroemeria flowers were improved by the use of salicylic acid treatment.

scholarly journals Antioxidant gene expression, chlorophyll, and starch content in Persian lime (Citrus latifolia Tanaka Ex Q. Jiménez) trees with HLB by application of elicitors of plant resistance

2021 ◽  
Vol 6 (2) ◽  
pp. 86-102
Author(s):  
Felipe Roberto Flores-de la Rosa ◽  
◽  
Ricardo Santillán-Mendoza ◽  
Cynthia Guadalupe Rodríguez-Quibrera ◽  
Antonio Martínez-Ruiz ◽  
...  
Keyword(s):  
Chlorophyll Content ◽  
Starch Content ◽  
Starch Accumulation ◽  
Economic Losses ◽  
Total Chlorophyll ◽  
Gamma Aminobutyric Acid ◽  
Antioxidant Genes ◽  
Spectrophotometric Methods ◽  
Total Chlorophyll Content ◽  
Randomized Design

Persian lime is economically important for Mexico. However, the disease known as Huanglongbing (HLB) causes chlorosis symptoms in the foliage that affect the growth and decreases the yield of the trees. The expression of chlorosis symptoms is associated with starch accumulation and inhibition of antioxidant activity. The objective of the present work was to determine the effect of the application of three resistance elicitors on the expression of three antioxidant genes and on starch and chlorophyll content in Persian lime trees with HLB. Salicylic acid (SA), jasmonic acid (JA) or gamma-aminobutyric acid (GABA) were applied every eight days for nine weeks using a completely randomized design and a one-way ANOVA was performed for the analysis. Expression of APX, CAT and SOD genes was quantified at week nine at four different times. Starch and total chlorophyll content was estimated every three weeks by spectrophotometric methods. The application of the elicitors significantly increased the expression of the three genes, with SA and GABA generating the greatest increase at different times of application. No difference was found in starch and total chlorophyll content at most of the times evaluated. The use of resistance elicitors is promising in the management of HLB in plots already infected, seeking to extend the productive life of the orchards and thus counteract the economic losses caused by HLB.

scholarly journals PENGARUH PERBANDINGAN IKAN KEMBUNG (Rastrelliger kanagurta) DENGAN SAYUR GONDA (Sphenoclea zeylanica Gaertner) TERHADAP KARAKTERISTIK BAKSO

2020 ◽  
Vol 9 (4) ◽  
pp. 426
Author(s):  
Brogina Mayank Dini ◽  
Luh Putu Trisna Darmayanti ◽  
I Ketut Suter
Keyword(s):  
Antioxidant Capacity ◽  
Protein Content ◽  
Water Content ◽  
Chlorophyll Content ◽  
Fat Content ◽  
Ash Content ◽  
Carbohydrate Content ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Randomized Design

This research was conducted with the aim to determine the effect of comparison of mackerel with gonda vegetable on meatballs characteristics and get a comparison of mackerel with specific gonda vegetable that can produce meatballs with the best characteristics. This study uses a Completely Randomized Design (CRD) with treatment that is the comparison of mackerel with gonda vegetable consisting of 6 levels: 100%: 0%; 95%: 5%; 90%: 10%; 85%: 15%; 80%: 20%; 75%: 25%. Each treatment was repeated 3 times so that obtained 18 units. The data is then analyzed by analysis of variance and if the treatment influences the proposed variable then it is continued by Duncan. The results showed that the comparison of mackerel and gonda vegetable had a very significant effect on water content, ash content, fat content, protein content, carbohydrate content, total chlorophyll content, antioxidant capacity, color (scoring), texture, aroma, overall acceptance and no significant effect for texture, aroma and taste. Comparison of 80% mackerel and 20% gonda vegetables had the best characteristics, with 62.99% water content, 1.56% ash content, 1.28% fat content, 8.75% protein content, 25.43% carbohydrate content, 18.39% total chlorophyll content, antioxidant capacity 25.63%. Sensory properties obtained are color (rather green, rather like), texture (rather like), flavor (liked) and overall acceptance (like).

scholarly journals The Effect of Organic Fertilizers on Growth and Yield of Water Spinach (Ipomoea reptans Poir)

2021 ◽  
Vol 3 (2) ◽  
pp. 37-44
Author(s):  
Selvia Dewi Pohan
Keyword(s):  
Plant Growth ◽  
Water Content ◽  
Chlorophyll Content ◽  
Organic Fertilizers ◽  
Chicken Manure ◽  
Growth And Yield ◽  
Total Chlorophyll ◽  
Cow Manure ◽  
Water Spinach ◽  
Total Chlorophyll Content

Water spinach (Ipomoea reptans Poir) had been identified as a nutritious vegetable with high demand in Indonesia. Besides, this plant also has been evidenced to play an important role in environmental cleaning as phytoremediator. The study about the effect of organic fertilizers on the growth and yield of water spinach (Ipomoea reptans Poir) has been conducted in the Green House of Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan. The study aims to investigate the most effective type of fertilizers and the dose for water spinach’s (Ipomoea reptans Poir) growth and yield. A Completely Randomized Factorial Design was designed for the experiment with two factors and three repetitions. The first factor is the type of fertilizer (chicken manure, cow manure, and compost), and the second factor is the dose of the fertilizer (1:1, 2:1, and 3:1). The parameters such as plant height, number of shoots, number of leaves, fresh weight, dry weight, leaf’s total chlorophyll content, and water content were measured to evaluate plant growth and yield. The General Linear Model used SPSS 21 programs was applied to analyze the collected data. Study results revealed that cow manure increased plant growth and yield significantly with dose 2:1 as the finest treatment, followed by dose 3:1. Cow manure also increased total chlorophyll content (8.0574c mg. L-1), with the most suitable dose was 2:1 (8.2807 mg. L-1). The plant’s water content tended to be high in chicken manure (93%), and the lower water content was in cow manure with dose 3:1 (87.5%).

Cell membrane stability and chlorophyll content variation in wheat (Triticum aestivum) genotypes under conditions of heat and drought

2016 ◽  
Vol 67 (7) ◽  
pp. 712 ◽  
Author(s):  
Shoaib Ur Rehman ◽  
Muhammad Bilal ◽  
Rashid Mehmood Rana ◽  
Muhammad Naveed Tahir ◽  
Muhammad Kausar Nawaz Shah ◽  
...  
Keyword(s):  
Heat Stress ◽  
Drought Stress ◽  
Cell Membrane ◽  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Stress Conditions ◽  
Seedling Stage ◽  
Total Chlorophyll ◽  
Cell Membrane Stability ◽  
Total Chlorophyll Content

Heat and drought are among the major obstacles confronting crop production under climate change. The present study was conducted to evaluate 50 diverse wheat genotypes for cell membrane stability (CMS) and chlorophyll content at seedling and anthesis stages under heat and drought stress conditions, to understand the effect of the two abiotic factors and to find promising genotypes for future breeding. Experiments were conducted in the glasshouse (seedling stage) and the field (anthesis stage). Analysis of variance showed significant variation (P ≤ 0.05) for all of the traits at seedling and anthesis stages. High levels of broad-sense heritability and genetic advance at 5% selection intensity indicated the presence of a high genetic component of variation and potential for genetic improvement through selection among the existing genetic variation. CMS showed a significant positive correlation with 1000-grain weight (TGW) under heat and drought conditions at both seedling and anthesis stages. Chlorophyll a/b ratio at seedling stage exhibited a significant negative correlation (r = –0.39, P < 0.05) with TGW under heat stress. Total chlorophyll content was significantly (r = 0.42, P < 0.05) correlated with TGW under heat stress at anthesis. Genotypes ETAD248 and ETAD7 showed the highest CMS and TGW values, whereas their chlorophyll a/b values were lowest, at both seedling and anthesis stages under heat and drought stress conditions. Higher CMS and total chlorophyll content, and lower chlorophyll a/b, were found to be useful indicators to identify genotypes with high TGW under heat and drought stress conditions. This study indicated the possibility of using seedling resistance as an indicator for later stage response in breeding for heat and drought resistance. The resistant genotypes identified can be used as potential germplasm in breeding programs.

scholarly journals Cultivation of Cress Involving Water Treated Under Different Atmospheres with Low-Temperature, Low-Pressure Glow Plasma of Low Frequency

Water ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2152
Author(s):  
Katarzyna Ciesielska ◽  
Wojciech Ciesielski ◽  
Damian Kulawik ◽  
Zdzisław Oszczęda ◽  
Piotr Tomasik
Keyword(s):  
Ascorbic Acid ◽  
Low Temperature ◽  
Chlorophyll Content ◽  
Tap Water ◽  
Low Frequency ◽  
Low Pressure ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Glow Plasma ◽  
Water Saturated

Watering cress with tap water treated for 30 min with low-temperature, low-pressure glow plasma of low frequency (LPGP) in the air (LPGPA), saturated with either nitrogen (LPGPN), CO2 (LPGPC) or methane (LPGPM), promoted the yield of crops. Their efficiency increased in the order LPGPA < LPGPN < LPGPM < LPGPC. The kind of water prior and after the treatment specifically influenced the fat and protein content in the watered cress and had no effect on the carbohydrate content. Watering cress with water saturated with N2 and CO2 slightly increased the total chlorophyll content, whereas watering with water saturated with CH4 significantly decreased it. Watering with plasma-treated water always resulted in an increase in the total chlorophyll content and subtly influenced the content of carotenoids and ascorbic acid. Watering cress with water saturated with particular gases influenced the bioaccumulation of cations and anions.

Total chlorophyll, growth and productivity of soybean [Glycine Max (L) Merril] under different levels and sources of phosphorus and plant growth regulators in south Rajasthan, India

2016 ◽  
Author(s):  
Shankar Lal Khaswan ◽  
R. K. Dubey ◽  
R. C. Tiwari ◽  
S. K. Dubey ◽  
Raj Kumari Chaudhary
Keyword(s):  
Plant Growth ◽  
Chlorophyll Content ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Total Biomass ◽  
Total Chlorophyll ◽  
Water Spray ◽  
Benzyl Adenine ◽  
Total Chlorophyll Content ◽  
Different Levels

An experiment was conducted at the Instructional Farm of Rajasthan College of Agriculture, Udaipur during Kharif 2009 and 2010 on medium clay loam soils to assess the influence of different levels and sources of phosphorus fertilization as well as plant growth regulators on productivity of soybean. Twenty seven treatments i.e. three levels (20, 30 and 40 kg P2O5 ha-1) and three sources (Single super phosphate or SSP; phosphorus rich organic manure or PROM and di-ammonium phosphate or DAP) of phosphorus in main plots and three PGRs (water spray, benzyl adenine 50 ppm and NAA 100 ppm) in sub plots of a split plot design having three replications. Results revealed that application of 40 kg P2O5 ha-1 recorded significantly higher pooled yield (q ha-1) of grain (25.95), stover (37.34) and total biomass (63.29); crop growth rate or CGR (g m-2day-1) between 30-60 days after sowing ( DAS)(18.83) and 61-90 DAS (11.96); total chlorophyll content at 45 DAS (2.26%) and 60 DAS (2.80%); Leaf area index(LAI) at 45 DAS (1.56) and 60 DAS (3.44) and green leaves plant-1 at 45 DAS (11.98) and 60 DAS (21.43). Among different phosphorus sources, SSP outperformed DAP and PROM on pooled yield (q ha-1) of grain (25.12), stover (36.23) and total biomass (61.35); CGR (g m-2day-1) between 30-60 DAS (11.22) and 61-90 DAS (10.94); total chlorophyll content at 45 DAS (2.26%) and 60 DAS (2.76%); LAI at 45 DAS (1.51) and 60 DAS (3.42) and green leaves plant-1 at 45 DAS (11.77) and 60 DAS (21.35). Foliar application of NAA recorded significantly higher pooled yield (q ha-1) of grain (24.23), stover (35.73) and total biomass (60.01) than benzyl adenine and water spray. However, variations recorded under NAA and benzyl adenine in CGR, total chlorophyll content, LAI and green leave plant-1at different stages of soybean crop were statistically at par.

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