The possibility of using bacteria Bacillus in the production of disinfectants

2020 ◽  
Vol 99 (5) ◽  
pp. 436-442
Author(s):  
Anastasia V. Ilyakova ◽  
N. V. Shestopalov ◽  
L. S. Fedorova ◽  
A. S. Belova
Keyword(s):  
Bacillus Subtilis ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Seeding Rate ◽  
Medical Institutions ◽  
Third Stage ◽  
Causative Agents ◽  
Bacillus Spores ◽  
Object Of Study ◽  
Healthcare Associated

Introduction. In recent years, healthcare-associated infections (HAIs) cause ever greater economic and social damage. Microorganisms circulating in medical institutions the causative agents of HAIs, become resistant to most antibiotics and many disinfectants. There is a need to create alternative mechanisms for controlling microorganisms. The purpose of the work is to determine the antagonistic activity of bacteria strains of the genus Bacillus in relation to test microorganisms, as a potential basis for the development of domestic detergents and disinfectants based on probiotics. Material and methods. The strains of Bacillus subtilis 2/10 VKPM B-2896; Bacillus subtilis VKPM B-1283; Bacillus licheniformis VKPM B-5397; Bacillus amyloliquefaciens VKPM B-5462 werethe main object of study. At the first stage of the studies, the antagonistic activity of the strains was studied by the well method, at the second stage of the studies, the antagonistic activity was studied at the test object, and at the third stage of the studies, the effectiveness of the composition containing B. subtilis VKPM B-1283, B.licheniformis VKPM B-5397 and sulfonol strains was studied. Results. As a result of studies, the antagonistic effect of bacilli against test microorganisms was revealed. When surfaces are contaminated with test microorganisms (S.aureus ATCC 6538-P, E.coli ATCC 10531, C. albicans ATCC 10231) and subsequent application of Bacillus spores, a significant reduction in the seeding rate with test microorganisms is observed after 24 hours by 97.07-100%. The use of B.subtilis VKPM B-1283 and B.licheniformis VKPM B-5397 spores together with a detergent component for treating surfaces contaminated with test microorganisms (S.aureus ATCC 6538-P, E.coli ATCC 10531, C.albicans ATCC 10231) provides a reduction in microbial seeding after 4 hours by 99.97 %. Conclusions. B.subtilis 2/10 VKPM B-2896, B.subtilis VKPM B-1283 and B.licheniformis VKPM B-5397 are promising for the use as probiotics, as the basis of detergents and disinfectants.

scholarly journals The possibility of using bacteria Bacillus in the production of disinfectants

2020 ◽  
Vol 99 (5) ◽  
pp. 436-442
Author(s):  
Anastasia V. Ilyakova ◽  
N. V. Shestopalov ◽  
L. S. Fedorova ◽  
A. S. Belova
Keyword(s):  
Bacillus Subtilis ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Seeding Rate ◽  
Medical Institutions ◽  
Third Stage ◽  
Causative Agents ◽  
Bacillus Spores ◽  
Object Of Study ◽  
Healthcare Associated

Introduction. In recent years, healthcare-associated infections (HAIs) cause ever greater economic and social damage. Microorganisms circulating in medical institutions the causative agents of HAIs, become resistant to most antibiotics and many disinfectants. There is a need to create alternative mechanisms for controlling microorganisms. The purpose of the work is to determine the antagonistic activity of bacteria strains of the genus Bacillus in relation to test microorganisms, as a potential basis for the development of domestic detergents and disinfectants based on probiotics. Material and methods. The strains of Bacillus subtilis 2/10 VKPM B-2896; Bacillus subtilis VKPM B-1283; Bacillus licheniformis VKPM B-5397; Bacillus amyloliquefaciens VKPM B-5462 werethe main object of study. At the first stage of the studies, the antagonistic activity of the strains was studied by the well method, at the second stage of the studies, the antagonistic activity was studied at the test object, and at the third stage of the studies, the effectiveness of the composition containing B. subtilis VKPM B-1283, B.licheniformis VKPM B-5397 and sulfonol strains was studied. Results. As a result of studies, the antagonistic effect of bacilli against test microorganisms was revealed. When surfaces are contaminated with test microorganisms (S.aureus ATCC 6538-P, E.coli ATCC 10531, C. albicans ATCC 10231) and subsequent application of Bacillus spores, a significant reduction in the seeding rate with test microorganisms is observed after 24 hours by 97.07-100%. The use of B.subtilis VKPM B-1283 and B.licheniformis VKPM B-5397 spores together with a detergent component for treating surfaces contaminated with test microorganisms (S.aureus ATCC 6538-P, E.coli ATCC 10531, C.albicans ATCC 10231) provides a reduction in microbial seeding after 4 hours by 99.97 %. Conclusions. B.subtilis 2/10 VKPM B-2896, B.subtilis VKPM B-1283 and B.licheniformis VKPM B-5397 are promising for the use as probiotics, as the basis of detergents and disinfectants.

Antagonistic activity of Bacillus subtilis strains isolated from various sources

2018 ◽  
Vol 8 (2) ◽  
pp. 354-364
Author(s):  
A. N. Irkitova ◽  
A. V. Grebenshchikova ◽  
A. V. Matsyura
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Wild Strain ◽  
Fish Farming ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
E Coli ◽  
Long Period ◽  
Test Culture ◽  
Agar Media

<p>An important link in solving the problem of healthy food is the intensification of the livestock, poultry and fish farming, which is possible only in the adoption and rigorous implementation of the concept of rational feeding of animals. In the implementation of this concept required is the application of probiotic preparations. Currently, there is an increased interest in spore probiotics. In many ways, this can be explained by the fact that they use no vegetative forms of the bacilli and their spores. This property provides spore probiotics a number of advantages: they are not whimsical, easily could be selected, cultivated, and dried. Moreover, they are resistant to various factors and could remain viable during a long period. One of the most famous spore microorganisms, which are widely used in agriculture, is <em>Bacillus subtilis</em>. Among the requirements imposed to probiotic microorganisms is mandatory – antagonistic activity to pathogenic and conditional-pathogenic microflora. The article presents the results of the analysis of antagonistic activity of collection strains of <em>B. subtilis</em>, and strains isolated from commercial preparations. We studied the antagonistic activity on agar and liquid nutrient medias to trigger different antagonism mechanisms of <em>B. subtilis</em>. On agar media, we applied three diffusion methods: perpendicular bands, agar blocks, agar wells. We also applied the method of co-incubating the test culture (<em>Escherichia coli</em>) and the antagonist (or its supernatant) in the nutrient broth. Our results demonstrated that all our explored strains of <em>B. subtilis</em> have antimicrobial activity against a wild strain of <em>E. coli</em>, but to varying degrees. We identified strains of <em>B. subtilis</em> with the highest antagonistic effect that can be recommended for inclusion in microbial preparations for agriculture.</p><p><em><br /></em><em></em></p>

scholarly journals CULTURAL MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF BACILLUS SUBTILIS STRAINS

2019 ◽  
pp. 58-62
Author(s):  
V. S. Rusaleyev ◽  
О. V. Pruntova ◽  
D. A. Vasilyev
Keyword(s):  
Bacillus Subtilis ◽  
Storage Period ◽  
Biological Properties ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Lag Phase ◽  
Probiotic Properties ◽  
Biochemical Characteristics ◽  
Species Characteristics ◽  
One Year

A decrease in therapeutic effect of some live lacto- and bifdobacteria-based drugs for veterinary use has been observed for the last 20 years that urges scientists to search for new microorganisms possessing probiotic properties. Many studies in this feld are focused onBacillus subtilisthat is widespread in the environment and non-pathogenic for animals and humans. Results of tests ofBacillus subtilisfor its biological properties and antagonistic activity aimed at optimization of methodical approaches for detection of strain with the highest antagonistic effect on some opportunistic microorganisms and their further use as probiotics are described. Cultural morphological and biochemical characteristics of the tested strains conformed to the species characteristics ofBacillus subtilis.Tested strains were nonpathogenic for white mice. Tests showed that spore biomass could be prepared both in liquid and on solid nutrient media. Methodically, spore biomass preparation in liquid nutrient medium is preferable. The tests showed that spores emerged from anabiosis non-uniformly and it depended on original seed spore storage period. Spore cultures stored less than one year emerged from anabiosis more quickly. It was found that the spores formed more readily when the cultures were aerated with oxygen as well as that lag-phase culture medium had a stimulating effect onBacillus subtilisspore germination.Bacillus subtilisstrains were found to have antagonistic effect onEscherichia coli, SalmonellaandStaphylococcus. Area of growth inhibition of the said bacteria was 15–20 mm. TestedBacillus subtilisstrains could be proposed for use as probiotics.

scholarly journals ANTAGONISTIC ACTIVITY OF ACTINOMYCETES ISOLATED FROM SALINE SOILS NEAR WITH KUMISI LAKE (GEORGIA)

2021 ◽  
pp. 55-59
Author(s):  
M. D. Tsulukidze ◽  
Z. Sh. Lomtatidze ◽  
Q. H. Mamulashvili
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Xanthomonas Campestris ◽  
Inhibitory Effect ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Pectobacterium Carotovorum ◽  
Rhizobium Radiobacter ◽  
Antibiotic Compounds ◽  
Escherichia Coli B

Aim. To study the antagonistic activity of actinomycetes isolated from soils adjacent to Kumisi Lake (Georgia). Methods. It was used 12 samples of actinomycetes isolated from the soils adjacent to Kumisi Lake. The antibiotic activity of actinomycetes was determined using test strains: Escherichia coli К-12 (B-3254), Bacillus subtilis 26 D, Xanthomonas campestris В-1459, Rhizobium radiobacter C58, Pectobacterium carotovorum EC1by the method of agar blocks. Results. It was revealed that actinomycete samples isolated from soils adjacent to Lake Kumisi shown the most significant inhibitory effect on test strains: Escherichia coli B-3254, Rhizobium radiobacter C58 and Xanthomonas campestris В-1459. The most resistant test-strains, to the studied actinomycetes, were Bacillus subtilis 26 D and Pectobacterium carotovorum EC1. Conclusion. Isolated strain 301 of actinomycetes shown a significant antagonistic effect on all test strains and could be potential for further studies spectrum and properties of antibiotic compounds.

scholarly journals Search for Promising Strains of Probiotic Microbiota Isolated from Different Biotopes of Healthy Cats for Use in the Control of Surgical Infections

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 667
Author(s):  
Pavel Rudenko ◽  
Yuriy Vatnikov ◽  
Nadezhda Sachivkina ◽  
Andrei Rudenko ◽  
Evgeny Kulikov ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
High Efficiency ◽  
Biological Marker ◽  
High Sensitivity ◽  
Antagonistic Activity ◽  
Adhesive Properties ◽  
Surgical Infection ◽  
Surgical Infections ◽  
Causative Agents ◽  
Inflammatory Processes

Despite the introduction of modern methods of treatment, the creation of new generations of antibacterial agents, and the constant improvement of aseptic and antiseptic methods, the treatment of purulent–inflammatory processes remains one of the most complex and urgent problems in veterinary practice. The article presents the results of the isolation of indigenous microbiota from various biotopes of healthy cats, as well as the study of their biological marker properties for the selection of the most optimal strains in probiotic medicines for the control of surgical infections. It was demonstrated that isolated cultures of bifidobacteria and lactobacilli, which we isolated, revealed high sensitivity to antibiotics of the β-lactam group (excepting L. acidophilus No. 24, L. plantarum “Victoria” No. 22, L. rhamnosus No. 5, L. rhamnosus No. 20, and L. rhamnosus No. 26, which showed a significant variability in sensitivity to antibacterial drugs of this group, indicating the great potential of these microorganisms) and resistance to aminoglycosides, lincosamides, and fluoroquinolones (with the exception of gatifloxacin, which showed high efficiency in relation to all lactic acid microorganisms). The adhesive properties of the isolated lactobacteria and bifidobacteria were variable, even within the same species. It was found that the B. adolescentis No. 23 strain of the Bifidobacterium genus, as well as the L. plantarum No. 8, L. plantarum “Victoria” No. 22, L. rhamnosus No. 6, L. rhamnosus No. 26, L. acidophilus No. 12, and L. acidophilus No. 24 strains of the Lactobacillus genus had the highest adhesive activity. Thus, when conducting a detailed analysis of the biological marker properties of candidate cultures (determining their sensitivity to antimicrobial agents, studying the adhesive properties, and antagonistic activity in relation to causative agents of surgical infection in cats), it was found that the most promising are L. plantarum “Victoria” No. 22, L. rhamnosus No. 26, and L. acidophilus No. 24.

scholarly journals Anti-Carcinogenic Glucosinolates in Cruciferous Vegetables and Their Antagonistic Effects on Prevention of Cancers

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2983 ◽  
Author(s):  
Prabhakaran Soundararajan ◽  
Jung Kim
Keyword(s):  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Side Chain ◽  
Cruciferous Vegetables ◽  
Related Factor ◽  
Nuclear Factor ◽  
Naturally Occurring ◽  
Therapeutic Properties ◽  
Responsive Element

Glucosinolates (GSL) are naturally occurring β-d-thioglucosides found across the cruciferous vegetables. Core structure formation and side-chain modifications lead to the synthesis of more than 200 types of GSLs in Brassicaceae. Isothiocyanates (ITCs) are chemoprotectives produced as the hydrolyzed product of GSLs by enzyme myrosinase. Benzyl isothiocyanate (BITC), phenethyl isothiocyanate (PEITC) and sulforaphane ([1-isothioyanato-4-(methyl-sulfinyl) butane], SFN) are potential ITCs with efficient therapeutic properties. Beneficial role of BITC, PEITC and SFN was widely studied against various cancers such as breast, brain, blood, bone, colon, gastric, liver, lung, oral, pancreatic, prostate and so forth. Nuclear factor-erythroid 2-related factor-2 (Nrf2) is a key transcription factor limits the tumor progression. Induction of ARE (antioxidant responsive element) and ROS (reactive oxygen species) mediated pathway by Nrf2 controls the activity of nuclear factor-kappaB (NF-κB). NF-κB has a double edged role in the immune system. NF-κB induced during inflammatory is essential for an acute immune process. Meanwhile, hyper activation of NF-κB transcription factors was witnessed in the tumor cells. Antagonistic activity of BITC, PEITC and SFN against cancer was related with the direct/indirect interaction with Nrf2 and NF-κB protein. All three ITCs able to disrupts Nrf2-Keap1 complex and translocate Nrf2 into the nucleus. BITC have the affinity to inhibit the NF-κB than SFN due to the presence of additional benzyl structure. This review will give the overview on chemo preventive of ITCs against several types of cancer cell lines. We have also discussed the molecular interaction(s) of the antagonistic effect of BITC, PEITC and SFN with Nrf2 and NF-κB to prevent cancer.

scholarly journals Isolation, Characterization, and Identification of Biological Control Agent for Potato Soft Rot in Bangladesh

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
M. M. Rahman ◽  
M. E. Ali ◽  
A. A. Khan ◽  
A. M. Akanda ◽  
Md. Kamal Uddin ◽  
...  
Keyword(s):  
Biological Control ◽  
Biological Control Agent ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Control Agent ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Antagonistic Bacteria ◽  
And Storage

A total of 91 isolates of probable antagonistic bacteria of potato soft rot bacteriumErwinia carotovorasubsp.carotovora(Ecc) were extracted from rhizospheres and endophytes of various crop plants, different soil varieties, and atmospheres in the potato farming areas of Bangladesh. Antibacterial activity of the isolated probable antagonistic bacteria was testedin vitroagainst the previously identified most common and most virulent soft rot causing bacterial strain Ecc P-138. Only two isolates E-45 and E-65 significantly inhibited thein vitrogrowth of Ecc P-138. Physiological, biochemical, and carbon source utilization tests identified isolate E-65 as a member of the genusBacillusand the isolate E-45 asLactobacillussp. The stronger antagonistic activity against Ecc P-138 was found in E-65in vitroscreening and storage potatoes. E-65 reduced the soft rot infection to 22-week storage potatoes of different varieties by 32.5–62.5% in model experiment, demonstrating its strong potential to be used as an effective biological control agent for the major pectolytic bacteria Ecc. The highest (62.5%) antagonistic effect of E-65 was observed in the Granola and the lowest (32.7%) of that was found in the Cardinal varieties of the Bangladeshi potatoes. The findings suggest that isolate E-65 could be exploited as a biocontrol agent for potato tubers.

scholarly journals Antifungal activity of several isolates of Trichoderma against Cladosporium and Botrytis

2018 ◽  
Vol 8 (1) ◽  
pp. 88-91 ◽  
Author(s):  
M. Skaptsov ◽  
S. Smirnov ◽  
M. Kutsev ◽  
O. Uvarova ◽  
T. Sinitsyna ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Trichoderma Harzianum ◽  
Its Region ◽  
Botanical Garden ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Soil Samples ◽  
Morphological Observation ◽  
The South

<p><em>Trichoderma</em> isolates (SSBGT07, SSBGT08, SSBGT09, SSBGT10) were isolated from the soil samples of the South-Siberian Botanical Garden and identified using morphological observation and ITS region analysis as <em>Trichoderma harzianum</em>, <em>T. asperellum, T. ghanense</em>, and <em>T. longibranchiatum</em>. Antagonistic activity against <em>Cladosporium </em>sp. and<em> Botrytis </em>sp. was evaluated <em>in vitro</em>. All isolates showed antagonistic effect by competition against <em>Cladosporium </em>sp. <em>T. asperellum </em>and <em>T. longibranchiatum</em> showed antagonism against <em>Botrytis </em>sp. All isolates showed hyper sporulation on the sclerotia of <em>Botrytis</em> sp. (except the <em>T. ghanense</em>) and colonies of the <em>Cladosporium</em> sp. Our study provides new isolates that affect the <em>Cladosporium </em>sp. and<em> Botrytis </em>sp.</p>

scholarly journals Current Pattern and Clinico-Bacteriological Profile of Healthcare Associated Infections (HAI) in an ICU Setting: An Observational Study

2021 ◽  
Author(s):  
Mradul Kumar Daga ◽  
Govind Mawari ◽  
Saman Wasi ◽  
Naresh Kumar ◽  
Udbhav Sharma ◽  
...  
Keyword(s):  
Age Groups ◽  
Healthcare Facility ◽  
Central Line ◽  
Multidrug Resistant ◽  
Gram Negative Bacteria ◽  
Healthcare Associated Infections ◽  
Acinetobacter Species ◽  
Gram Negative ◽  
Causative Agents ◽  
Healthcare Associated

Abstract Objective To understand the pattern and types of healthcare associated infections (HAI) at our healthcare facility, and to determine the common causative agents and their antibiotic susceptibility profile. Methods One hundred consecutive patients diagnosed with HAI were enrolled and monitored; the causative organisms isolated on culture were recorded and their sensitivity profile was generated. Results Of the 100 patients diagnosed with HAI (mean age ± SD being 42 ± 17 years), there were a total of 110 hospital acquired infections with 10 patients having two infections each. Out of 100 patients with HAI, 69 patients had ventilator associated pneumonia (VAP), 21 patients had catheter associated urinary tract infection (CAUTI) patients, and 20 patients had central line associated bloodstream infection (CLABSI). There were 10 patients with both VAP and CAUTI. All of the HAIs were device associated. A total of 76 pathogens were isolated on culture. No organism was isolated in 40 HAI. Majority (94.7%) of the organisms isolated from HAIs were gram-negative bacteria and all were multidrug resistant. Seventy-seven of the enrolled patients expired while 23 were discharged from the hospital Conclusions Our study demonstrated that HAIs occur in patients of all age groups; younger patients are not spared. Majority of the HAIs were caused by multidrug resistant gram-negative bacteria and were associated with high patient mortality. Acinetobacter species was the most common organism associated with HAI.

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