scholarly journals SEROLOGICAL STUDY OF BOVINE LEUKOSIS IN IRAQ LOCAL BREED AND IMPORTED COW

2022 ◽  
Vol 21 (1) ◽  
pp. 137-142
Author(s):  
Afaf Abd - Al-Rahmain Yousif
Keyword(s):  
Specific Antibody ◽  
Serological Study ◽  
Local Breed ◽  
Blood Samples ◽  
Bovine Leukosis

Two hundreds and sixteen blood samples from Iraqi local breed cows in Baghdad, Basrah, Diala provinces and 144 blood samples from imported cows in three large cows stations were collected. All samples were tested by ELISA for the detection of specific antibody to bovine leucosis.  Results revealed the presence of antibodies of 8.4 % in imported cows only. Whereas the blood samples from the local animals included in this study were negative.

scholarly journals Serological diagnosis of Toxoplasmosis by specific antibody in camels of Wasit province, Iraq

2018 ◽  
Vol 1 (29) ◽  
pp. 412-423
Author(s):  
Basim Mohammed Hanon
Keyword(s):  
Public Health ◽  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Specific Antibody ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Samples ◽  
Zoonotic Parasite

Background: toxoplasma gondii is a zoonotic parasite, more added a major public health is worldwide because have high distribution in livestock. Objectives: the main aim of this study determine the occurrence of the seroepidemiological toxoplasmosis in camels in waist province of Iraq from November 2016 to April 2017. Materials and Methods: blood samples collected of animals randomly were included six different groups of animals were diagnosed by A Latex agglutination test (LAT) and indirect enzyme linked immunosorbent assay (ELISA) kits.

scholarly journals Viral haemorrhagic disease of rabbits and human health

1998 ◽  
Vol 121 (2) ◽  
pp. 409-418 ◽  
Author(s):  
J. A. CARMAN ◽  
M. G. GARNER ◽  
M. G. CATTON ◽  
S. THOMAS ◽  
H. A. WESTBURY ◽  
...  
Keyword(s):  
Biological Control ◽  
New Zealand ◽  
Enzyme Immunoassay ◽  
Specific Antibody ◽  
Field Trials ◽  
Blood Samples ◽  
Organ Systems ◽  
Occupationally Exposed ◽  
Haemorrhagic Disease ◽  
Very High

Viral haemorrhagic disease of rabbits (VHD), a potential biological control for wild rabbits in Australia and New Zealand, escaped from quarantined field trials on Wardang Island and spread to the mainland of Australia in October 1995. This study looked for any evidence of infection or illness in people occupationally exposed to the virus. Two hundred and sixty-nine people were interviewed and 259 blood samples were collected. Exposures to VHD-infected rabbits ranged from nil to very high. No VHD antibodies were detected in any of the 259 sera when tested by VHD competitive enzyme immunoassay, which had been validated with 1013 VHDV-specific antibody negative sera. A questionnaire designed to elicit symptoms of disease in a range of organ systems found no significant differences between illness in those exposed and those not exposed to VHD, nor could an association be found between exposure and subsequent episodes of illness. The findings are consistent with the view that exposure to VHD is not associated with infection or disease in humans.

scholarly journals Dynamics of Schmallenberg virus infection within a cattle herd in Germany, 2011

2013 ◽  
Vol 142 (7) ◽  
pp. 1501-1504 ◽  
Author(s):  
K. WERNIKE ◽  
C. SILAGHI ◽  
M. NIEDER ◽  
M. PFEFFER ◽  
M. BEER
Keyword(s):  
Virus Infection ◽  
Serum Sample ◽  
Specific Antibody ◽  
Core Region ◽  
Cattle Herd ◽  
Schmallenberg Virus ◽  
Cattle Farm ◽  
Blood Samples ◽  
The Core ◽  
Antibody Elisa

SUMMARYIn late 2011, the insect-transmitted Schmallenberg virus (SBV) emerged in Europe. In this study, a cattle farm located in the core region of the epidemic was closely monitored between May 2011 and January 2012. Up to the end of September every tested serum sample was negative by an SBV-specific antibody ELISA, suggesting the absence of an infection before autumn 2011. Around the end of September/beginning of October SBV genome was detected in blood samples of some animals, and a few cows exhibited fever during that period. Starting at the end of September the first cows seroconverted; the within-herd prevalence reached 100% within barely 1 month. Consequently, SBV spread rapidly in the tested herd during the vector season of 2011.

scholarly journals Measles virus-specific antibody levels in Sudanese infants: a prospective study using filter-paper blood samples

2005 ◽  
Vol 134 (1) ◽  
pp. 79-85 ◽  
Author(s):  
S. A. IBRAHIM ◽  
A. ABDALLAH ◽  
E. A. SALEH ◽  
A. D. M. E. OSTERHAUS ◽  
R. L. DE SWART
Keyword(s):  
Cord Blood ◽  
Specific Antibody ◽  
Filter Paper ◽  
Measles Virus ◽  
Dried Blood Spots ◽  
Birth Cohort Study ◽  
Blood Samples ◽  
Heel Prick ◽  
A Prospective Study ◽  
Antibody Levels

SUMMARYWe conducted a prospective birth cohort study in rural Sudan to assess measles virus (MV)-specific antibody levels at different time points in infancy. Dried blood spots were collected on filter paper at birth (cord blood) and at ages 6, 12 and 24 months (heel-prick). Maternally derived MV-specific antibody levels were high in cord blood samples, but at the age of 6 months had dropped below cut-off values in half of the infants. By extrapolation it was concluded that the current Expanded Programme of Immunization (EPI) target age for measles vaccination of 9 months was an appropriate choice for this area. At the age of 24 months acquired MV-specific antibodies were detected in 65–85% of the cohort, which corresponded well with the 79% of infants reported to be vaccinated by this age. This study demonstrates the usefulness of filter paper blood samples for seroepidemiological studies in developing countries.

scholarly journals Conformation of the clinical diagnoses of some malnutrition diseases in local and Shammi goats in Baghdad province

2012 ◽  
Vol 36 (1) ◽  
pp. 8-14
Author(s):  
Al-Shawi A.F. S.
Keyword(s):  
Body Temperature ◽  
Normal Values ◽  
Mean Body Temperature ◽  
Local Breed ◽  
Blood Samples ◽  
Clinical Diagnoses ◽  
The Mean ◽  
Phosphorus And Potassium ◽  
Hb Concentration ◽  
The Relationship

The relationship between the clinical singes and some of the hematological and biochemical values in 126 cases of local breed and Shammi breed goats (from both sexes) diagnosed as cases of malnutrition from 230 goats examined .The clinical examination include (beside the general inspection and case history) body temperature, respiratory and pulse rates, mucus membranes skin and coat. Examination of the blood samples included RBCs count, Hb concentration, PCV % and values of cupper, magnesium, phosphorus, and potassium. The results showed that the mean body temperature of these goats was lower than in normal goats, while the respiratory and pulse rates were higher .Examination of blood revealed decrease in the means of RBCs counts ,Hb concentration and PCV % in the clinically diagnosed cases , and the decrease was more sever in local goats . However the results indicated lower values of cupper, magnesium, phosphorus, and potassium in comparison with normal values in goats. The ratio of cupper deficiency was the highest followed by phosphorus, magnesium and potassium and the ratio of malnutrition was high in Baghdad province. It was in Shammi breed higher than in local breed.

scholarly journals VARIASI GENETIK KAMBING BENGGALA DI KABUPATEN MANGGARAI BARAT BERDASARKAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA

2018 ◽  
Vol 5 (2) ◽  
pp. 222
Author(s):  
Suhendra Pakpahan ◽  
Wayan Tunas Artama ◽  
Rini Widayanti ◽  
I Gede Suparta Budisatria
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Rural Communities ◽  
Genetic Similarity ◽  
Random Amplified Polymorphic Dna ◽  
Extended Period ◽  
Sample Population ◽  
Natural Conditions ◽  
Local Breed ◽  
Blood Samples

Genetic Variation of Benggala Goats in West Manggarai Regency Based on Random Amplified Polymorphic DNA Method ABSTRACTIndonesia has several types of local goats that have had an extended period of adaptation to the natural conditions in Indonesia. Goat is one of the most important germplasm in supporting the economy of rural communities. Benggala is a local breed of goat originating from Flores Island, East Nusa Tenggara province and has distinctive characteristics. The RAPD technique has several advantages and has been widely used in studies of the genetic diversity of goats. A total of 50 blood samples of Benggala goats were taken from four sub-districts in West Manggarai Regency. This study was conducted to estimate genetic variations of Benggala goats using OPA-6 and OPA-16 primers. The OPA-6 primer consisted of 0-11 bands, while the OPA-16 primer consisted of 0-7 bands. The total bands produced on the OPA-6 primer from all samples was 456, whilst OPA-16 primer was 314. The lowest genetic similarity between individuals of Benggala goats was 44% from the sample K46. Based on the sample population, the average genetic similarity level was 72%. These results show that the genetic diversity of Benggala goats is low.Keywords: Benggala  goat, genetic similarity,genetic variation, RAPD, West Manggarai ABSTRAKIndonesia memiliki beberapa jenis kambing lokal yang memiliki periode adaptasi yang panjang dengan kondisi alam di Indonesia. Kambing merupakan salah satu plasma nutfah yang sangat penting dalam mendukung perekonomian masyarakat pedesaan. Benggala adalah jenis kambing lokal yang berasal dari pulau Flores, propinsi Nusa Tenggara Timur dan kambing Benggala memiliki ciri khas. Teknik RAPD memiliki beberapa keunggulan dan telah banyak digunakan pada studi keragaman genetik kambing. Total 50 sampel darah kambing Benggala yang diambil dari empat kecamatan di Kabupaten Manggarai Barat. Penelitian ini dilakukan untuk menguji variasi genetik kambing Benggala dengan menggunakan primer OPA-6 dan OPA-16. Primer OPA-6 terdiri dari 0-11 band, sedangkan primer OPA-16 terdiri dari 0-7 band. Total jumlah pita yang dihasilkan pada primer OPA-6 dari semua sampel adalah 456, sementara primer OPA-16 adalah 314. Kemiripan genetik terendah antara individu-individu kambing Benggala adalah 44% dari sampel K46. Berdasarkan populasi sampel, tingkat kemiripan genetik rata-rata adalah 72%. Hasil ini menunjukkan bahwa keragaman genetik kambing Benggala tergolong rendah.Kata Kunci: kambing Benggala, kemiripan genetik, Manggarai Barat , RAPD, variasi genetik

scholarly journals Conformation of the clinical diagnoses of some malnutrition diseases in local and Shammi goats in Baghdad province

2012 ◽  
Vol 36 (1) ◽  
pp. 8-14
Author(s):  
Al-Shawi A.F. S.
Keyword(s):  
Body Temperature ◽  
Normal Values ◽  
Mean Body Temperature ◽  
Local Breed ◽  
Blood Samples ◽  
Clinical Diagnoses ◽  
The Mean ◽  
Phosphorus And Potassium ◽  
Hb Concentration ◽  
The Relationship

The relationship between the clinical singes and some of the hematological and biochemical values in 126 cases of local breed and Shammi breed goats (from both sexes) diagnosed as cases of malnutrition from 230 goats examined .The clinical examination include (beside the general inspection and case history) body temperature, respiratory and pulse rates, mucus membranes skin and coat. Examination of the blood samples included RBCs count, Hb concentration, PCV % and values of cupper, magnesium, phosphorus, and potassium. The results showed that the mean body temperature of these goats was lower than in normal goats, while the respiratory and pulse rates were higher .Examination of blood revealed decrease in the means of RBCs counts ,Hb concentration and PCV % in the clinically diagnosed cases , and the decrease was more sever in local goats . However the results indicated lower values of cupper, magnesium, phosphorus, and potassium in comparison with normal values in goats. The ratio of cupper deficiency was the highest followed by phosphorus, magnesium and potassium and the ratio of malnutrition was high in Baghdad province. It was in Shammi breed higher than in local breed.

scholarly journals Clinical and Biochemical Profile of Iraqi Local Breed Cows During Pregnancy and Early Lactation

2020 ◽  
Vol 44 ((E0)) ◽  
pp. 51-56
Author(s):  
Al-Bayati O.A. Shareef
Keyword(s):  
Biochemical Parameters ◽  
Jugular Vein ◽  
Late Pregnancy ◽  
Early Lactation ◽  
Biochemical Profile ◽  
Local Breed ◽  
Blood Samples ◽  
Significant Difference ◽  
Calcium Iron

This study was conducted in Al-Alam region, which is located in Salah Al-Din Governorate, on first month pregnant Iraqi local cows (n-10). The follow up extended from the first month of pregency up to the end of the first month post-parturituion during that some clinical and biochemical parameters were measured in the serum. Blood samples from the jugular vein of cows were collected monthly for the whole period of experiment and divided into four stages: early pregnancy, mid pregnancy, late pregnancy and early lactation immediately after birth. It was observed that the temperature, respiration rate, and heart rate increased gradually and significantly (P ≤ 0.05) with the progress of pregnancy reaching to its highest value in the last period of pregnancy and decreased after birth. The last trimester of pregnancy and the early lactation were also showed a significant decrease (P ≤ 0.05) of the phosphorus, calcium, iron and copper concentrations compared to the first and second trimesters of the pregnancy howecer, significant increasees in other biochemical values (P ≤ 0.05) were observed in urea and creatinine concentrations in the last trimester of pregnancy and early lactation. In addition to a significant decrease (P ≤ 0.05) of the ratios of concentrations of glucose, total protein, albumin and glubulin in the last period of pregnancy compared to other periods. The decrease in the value of glubulin continued until the early birth period while the study did not showed any significant difference in the concentration of bilirubin between durations. Finally, values of the ALT, AST and ALP enzymes showed significant increases (P ≤ 0.05) in thier concentrations in the last period of pregnancy. It is concluded that possible changes in the biochemical parameters of the local Iraqi cows blood during pregnancy and early lactation are existed

scholarly journals Study the effects of naturally acquired canine Dirofil Ariasis on some hematological and biochemical parameters

2017 ◽  
Vol 41 (1) ◽  
pp. 104-108
Author(s):  
Amal Hassen Atyha
Keyword(s):  
Biochemical Parameters ◽  
Clinical Signs ◽  
Reference Ranges ◽  
Pathological Changes ◽  
Affected Animal ◽  
Local Breed ◽  
Blood Samples ◽  
Blood Constituent ◽  
Serum Biochemical ◽  
Hematological And Biochemical Parameters

     The present study was aimed to check the amount of variation in some hematologic and biochemical parameters accompanied with natural acquired canine dirofilariasis. Blood samples were collected from sixty five stray dogs (5-10) years old belong to local breed dog in the villages of Al-Hindya area/ Karbala Governorate, The affected animal showed differences in hematological and biochemical values as compared with reference ranges. In conclusion, the disease showed no significant clinical signs although many pathological changes in some blood constituent with serum biochemical parameters were observed, before euthanasia for dogs.

scholarly journals Allopregnanolone concentrations and premenstrual syndrome

2000 ◽  
pp. 269-273 ◽  
Author(s):  
P Monteleone ◽  
S Luisi ◽  
A Tonetti ◽  
F Bernardi ◽  
AD Genazzani ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Specific Antibody ◽  
Premenstrual Syndrome ◽  
Luteal Phase ◽  
Test Design ◽  
Normal Range ◽  
Serum Progesterone ◽  
Blood Samples ◽  
Gaba A ◽  
Psychological Conditions

OBJECTIVE: To evaluate basal allopregnanolone and progesterone in both phases of the menstrual cycle in women suffering from premenstrual syndrome (PMS) and their response to a GnRH test. DESIGN: We selected 56 women (28 patients with PMS and 28 controls) aged between 18 and 32 years. Blood samples were drawn in both follicular and phases. Twenty-eight women (14 patients with PMS and 14 controls) underwent a GnRH test in the luteal phase. METHODS: We evaluated allopregnanolone by RIA, using a specific antibody. Serum progesterone and oestradiol were determined using a commercially available RIA kit. RESULTS: Luteal phase allopregnanolone concentrations were significantly lower in patients with PMS than in controls. Progesterone concentrations were significantly lower in patients with PMS in both the follicular and the luteal phase. Serum oestradiol concentrations were in the normal range in both groups of women, although slightly greater in those with PMS. Allopregnanolone and progesterone responses to a GnRH test were significantly blunted in women with PMS. CONCLUSIONS: Diminished concentrations of allopregnanolone and progesterone, its precursor, and a blunted response to the GnRH test lead us to hypothesise that patients with PMS may suffer from an inadequate production of ovarian neuroactive steroids, especially in the luteal phase. This would lead to an impaired anxiolytic GABA(A)-mediated response in stressful physiological and psychological conditions, and may in part explain various psychoneuroendocrine symptoms that arise during PMS.

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