scholarly journals Histopathological and diagnostic study of Toxoplasmosis in human and sheep by using ELISA in Kut city

2017 ◽  
Vol 40 (2) ◽  
pp. 94-99
Author(s):  
Zainab R. Zghair
Keyword(s):  
Blood Vessels ◽  
Histopathological Examination ◽  
Inflammatory Cells ◽  
Elisa Test ◽  
Control Group ◽  
Diagnostic Study ◽  
Total Percentage ◽  
Multiple Variable ◽  
Cardiac Muscle Fibers ◽  
Normal Men

     This study is designed to diagnosis blood Toxoplasmosis in human (women and men) and sheep (male and female) by using Elisa test; as well histopathological examination of heart, liver and lung     of sheep were also done, internal organs were also obtained heart, liver and lung. Women diagnosis was performed by collection 96 samples from aborted women ageing from 15-45 years and divided into three groups; first group 15-25 years involved 50 sample, second group 25-35 years include 37 samples; and the third group 35-45 years included 9 samples. The results of Elisa test showed six positive of IgG, (two for each group) and one positive IgM for the second group, a significant (P<0.05) difference in the first and third groups between IgG and IgM was found. The total percentage of the infection 4, 8.1and 22.2 for the first, second and third groups respectively, and Total of 96 blood samples were collected from men with different chronic diseases and were divided into four groups, (first, second and third) each containing 25 samples and suffering from Thalasemia, renal failure and cancer respectively; of them 21 samples collected from normal men as fourth control group. The results of Elisa diagnostic test indicated 9, 9, 7 and 2 positive of IgG for first, second, third and fourth groups respectively, and 1, 2, 0 and 1 positive of IgM. The total percentage of Toxoplasmosis infection 38.4, 44, 28 and 14.28 for the first, second, third and fourth groups respectively, with a difference between them except the second group appeared significantly P(0.05). 92 samples of sheep blood were collected from male 46 and 46 female. The results of Toxoplasmosis showed one case positive IgG for female and one for male only. The histopathological examination of sheep positive case with chronic infection revealed pathological changes in the organs, the heart showed multiple variable sizes of parasite cysts embedded and scatter between cardiac muscle fibers, in addition to aggregation of inflammatory cells around the blood vessels forming nests of inflammatory cells with congestion of blood vessels in liver and lung. In conclusion that the infection with Toxoplasmosis in Kut city revealed the percentage of infection in men more than the aborted women, also it reported few cases of infection in sheep.

scholarly journals Evaluation the exposure of Formalin as a Disinfectant For Poultry House on Hematological, Biochemical Parameters and Histopathological Examination in Broiler

2013 ◽  
Vol 12 (2) ◽  
pp. 144
Author(s):  
M. H. AL-Saeed
Keyword(s):  
Blood Vessels ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Histopathological Examination ◽  
Inflammatory Cells ◽  
Treated Group ◽  
Control Group ◽  
Alveolar Cells ◽  
Poultry House ◽  
Wbc Count

This study was done to evaluate exposure to inhalation of formalin as a disinfectant for poultry house on hematological, biochemical parameters and histopathological examination in the broiler. The used (24) chicken divided into two groups, every group included (12) chicken. The first group (control) and second group exposure to formalin by inhalation for 2 weeks. Blood samples were collected at end of each week for analyzed some hematological such as (PCV, Hb, RBCs, total WBC counts and differential WBC count) and biochemical parameters such as (total protein, albumin, globulin and bilirubin) at the end of each week after 1wk scarified of half number of broiler and remove organs such as (lung, heart, and liver) for histopathological examination and end of 2wk scarified the reminder number of broiler. In our study, the hematological changes revealed significant decreases in PCV, Hb, RBCs, and WBC count after 1wk and 2wk compared with the control group. The total protein, albumin, and globulin showed significant decreases while bilirubin showing significant increasing after 1wk and 2wk of treated group compared with the control group. The differential WBC count showed significant increasing into neutrophils, and monocytes after 1wk while there was significant increasing into eosinophils, basophils, and neutrophils after 2wk compared with the control group. There was significantly decreasing into lymphocytes eosinophils and basophils after 1wk of treated groups but after 2wk the monocytes and lymphocytes that showed significant decreasing. The histopathological examination of lung in treated group after 1wk revealed highly changes that represent by congestion of blood vessels, hemorrhages with thickening of blood vessels walls, thickening into alveolar walls, alveoli filled with erythrocytes and fibrin, hemosiderosis, and hyperplasia of alveolar cells into treated group compared with control group, while after 2wk the changes was emphysema (destruction of alveolar walls) only. The heart revealed congestion of blood vessels after 2weeks. The liver revealed more changes that represented by congestion of central veins and necrosis in the hepatocytes, fibrosis, and hyperplasia into the portal area of the liver tissues compared with control group. Changes into liver after 2wk of treated group showed acute inflammation, congestion of blood vessels, hemorrhage and degeneration of hepatocytes with necrosis into some areas. After 1wk the heart show slightly changes includes congestion of blood vessels and slightly infiltration of inflammatory cells and hemorrhage compared with control group, but after 2wk the heart shows more infiltration of inflammatory cells with fibrosis of treated group compared with control group.

scholarly journals Effects of Adenosine A2A Receptor Agonist on Histopathology of Some Oral Tissues in Rabbits

2020 ◽  
Vol 11 (4) ◽  
pp. 5173-5178
Author(s):  
Faehaa Azher Al-Mashhadane
Keyword(s):  
Treatment Group ◽  
Salivary Glands ◽  
Blood Vessels ◽  
Rabbit Model ◽  
Inflammatory Cells ◽  
The Body ◽  
Control Group ◽  
Serum Samples ◽  
Tissue Samples ◽  
Group 15

Adenosine is a protective regulator that act endogenously to restore equilibrium of cellular energy in response to tissue trauma. It can perform such function of different systems in the body by activation of adenosine receptors. Study the effects of systemic administration of the adenosine on tongue and salivary glands tissues in the rabbit model. Thirty male rabbits of body weight of 1.5 ± 0.25kg were included in the study. In control group (15 animals), one ml of distilled water was injected intraperitoneally while in treatment group (15 animals) were injected by adenosine intraperitoneally at a dose of one mg/ml, All animals were sacrificed after 30 days. Serum samples were separated and used for analysis of adenosine deaminase (ADA)and glutathione(GSH). Tissue samples sections from tongue and salivary glands were stained with hematoxylin-eosin (H&E) and examined under a light microscope for histological changes by a blinded pathologist. Histological sections in treatment group showed congestion of blood vessels and infiltration of inflammatory cells with mild hemorrhage among acini of salivary glands. Increased level of adenosine in the body microenvironment may affect tongue and salivary glands tissues by modulating some processes including inflammation and blood vessels.

scholarly journals Teratogenic effects of Metoprolol in the chick embryos

2015 ◽  
Vol 39 (1) ◽  
pp. 49-55
Author(s):  
Abbas Razzaq Abed
Keyword(s):  
Histopathological Examination ◽  
Inflammatory Cells ◽  
Heart Tissue ◽  
Control Group ◽  
Control Groups ◽  
Teratogenic Effects ◽  
Fourth Group ◽  
The Third ◽  
Significant Difference ◽  
T1 And T2

     This study was conducted to demonstrate the effect of Metoprolol on chicken embryos during the period of cuddling automated .This study was completed in Babylon hatchery dedicated to the production of chick chickens, which is located in the province of Babylon. One hundred eggs (Belgian origin, CZ 1924) were divided into equal four groups. The first group injected eggs by Metaprolol at dose 10 mg /70kg BW, and the second group eggs was injected by Metoprolol at dose 15 mg /70kg BW, while eggs in the third group was injected by physiological normal saline only and the fourth group did not inject their eggs any material and considered the control group and all the eggs used in this experiment to take on the fourth day of cuddling. The results of this study showed a decrease in the percentage hatching in groups injected with Metoprolol (T1 and T2 groups, 4% and 13%, respectively) compared to the T3 and the control groups. Also showed the results of the current study, no significant difference at the level of (P <0.05) in the weights of embryos after hatching. While histopathological examination showed the presence of pathological lesions in the heart tissue in injected eggs groups by Metoprolol (T1 and T2 groups) and included these changes infiltration of inflammatory cells, thickening in epicardium and the presence of vacuolation in heart tissue with the appearance of edema, heart tissue damage, congestion in the blood vessels and the occurrence of hemorrhage.

scholarly journals Efficacy of adjuvant intrastromal and combination of intrastromal and intracameral voriconazole in Aspergillus fumigatus-induced moderate fungal keratitis in rabbits

2021 ◽  
Vol 30 (1) ◽  
pp. 13-9
Author(s):  
Dyah Tjintya Sarika ◽  
Melva Louisa ◽  
Anna Rozaliyani ◽  
Evelina ◽  
Made Susiyanti
Keyword(s):  
Aspergillus Fumigatus ◽  
Clinical Response ◽  
Histopathological Examination ◽  
Inflammatory Cells ◽  
Fungal Keratitis ◽  
Control Group ◽  
Corneal Tissue ◽  
Epithelial Defect ◽  
Treatment Groups

BACKGROUND There is no in vivo evidence for the effectiveness of adjuvant intrastromal and combination of intrastromal and intracameral voriconazole (VCZ) for treating Aspergillus fumigatus keratitis. This study aimed to compare the efficacy of both agents against it.  METHODS A randomized, masked, controlled experimental study was conducted on 11 albino New Zealand white rabbits in which moderate fungal keratitis was induced by inoculating spores of A. fumigatus to the cornea. The rabbits were allocated into 3 groups: 50 μg/0.1 ml intrastromal VCZ injection, 50 μg/0.1 ml intrastromal VCZ and intracameral VCZ injections, and topical VCZ (control). The treatment was given 5 days after inoculation. Epithelial defect, infiltrate size, corneal ulcer depth, and hypopyon were evaluated clinically. Histopathological and mycological examinations were also done 14 days after treatment.  RESULTS All rabbits in the adjuvant treatment groups demonstrated a tendency of a better clinical response with decreasing size of epithelial defect (p = 0.679) and infiltrate (p = 0.755) than in the control group. Direct microscopy, corneal culture, and chop corneal tissue culture were still positive in most of the rabbits from all groups. Histopathological examination showed an increase of inflammatory cells after treatment in all groups, especially in rabbits which were inoculated with A. fumigatus spores in both eyes.  CONCLUSIONS An adjuvant combination of intrastromal and intracameral VCZ showed a tendency of better clinical response for A. fumigatus-induced moderate fungal keratitis in rabbits. 

scholarly journals Toxicopathological effects of lead acetate on the brain of male mice

2012 ◽  
Vol 36 (0E) ◽  
pp. 340-346
Author(s):  
Bassim M. Jwad
Keyword(s):  
Body Weight ◽  
Blood Vessels ◽  
Lead Acetate ◽  
Histopathological Examination ◽  
Clinical Signs ◽  
Physiological Saline ◽  
Inflammatory Cells ◽  
Stomach Tube ◽  
Toxic Dose ◽  
The Brain

The aim of the present study is to determine the influence of different doses of lead acetate on the central nerves system. 25 Swiss strain white mice were used, each weighing about 30 –32 g, divided into three groups, first group (n=10) treated with 0.5 ml. contain 150 mg/kg/body weight lead acetate via stomach tube daily for 40 days, second group (n=10) treated with 0.5 ml. contain 300 mg/kg/body weight lead acetate via stomach tube daily for 40 days. While the third group (n=5) served as control and were given mammalian physiological saline. Clinical signs were reported during the course of the study, then at day 40 post treatment , all animal were sacrificed and post mortem examination was done and any gross lesions were reported, then the pieces of brain was fixed in 10% formalin for 72 hours .The pathological results showed congestion of cerebellum and cerebrum of both treated group but the 2nd group was more sensitive. Histopathological examination of 1st group expressed moderate pathological lesions, characterized by extracellular edema around neuron cells and Virchow Robbin space , as well as proliferation of astrocyets in the white matter , also central chromatolysis of neurons and Nissle granules with homogenous pink their cytoplasm in 1st G. while in 2nd G the main lesions characterized by severe congestion of blood vessels with inflammatory cells infiltration in the lumen of arachnoids' space and brain parenchyma as well as hemorrhage with aggregation of microglia in the wall of blood vessels which characterized by round shape and clear cytoplasm (microgliosis). Also severe neuron degeneration, with Alzheimer's type-II astrocyets are reported in other animals characterized by pairs observed surrounded by clear space. And there is no clear pathological lesion in control group.In conclusion the present study investigated that the lead acetate affected on the brain tissue, and the degree of influence depended on the concentration of the toxic dose.

scholarly journals Protective effect of melatonin on the rat lung following exposure to 900-MHz electromagnetic field: a stereological and histopathological study

2021 ◽  
Vol 38 (2) ◽  
pp. 55-60
Author(s):  
Ahmad Yahyazadeh ◽  
Elfide Gizem Kıvrak ◽  
Gülüna Erdem Koç
Keyword(s):  
Electromagnetic Field ◽  
Blood Vessels ◽  
Histopathological Examination ◽  
Marked Change ◽  
Female Rats ◽  
Control Group ◽  
Albino Rats ◽  
Histopathological Study ◽  
Rat Lung ◽  
The Mean

Long-term use of cell phone emitting electromagnetic field (EMF) has increased concerns regarding histopathological change in vital organs. Our present study was aimed to investigate the effect of 900 megahertz (MHz) EMF on the rat lungs, as well as the possible efficacy of melatonin (MEL) on lung tissues. Fifteen female Wistar albino rats were randomly selected and assigned into five groups as follow: control (CONT), SHAM, EMF, melatonin (MEL) and EMF+MEL group. Subsequently, female rats were then mated, and pregnant rats underwent the experimental application for 21 days. After parturition, 6 pups (2 pups from each mother) were randomly chosen and maintained for 4 weeks. At the end of the 4th week, the lung tissues of all groups were used for the stereological analysis and histopathological examination. We found that the mean volumes of the alveoli, bronchioles and blood vessels were significantly lower in the EMF group compared to the control group (p<0.05). In the EMF+MEL group, the mean volumes of the alveoli, bronchioles and blood vessels were significantly increased compared to the EMF group. Our histopathological results showed marked change in the lung tissues. We speculated that exposure to EMF caused the damage to the rat lung tissues, and that MEL administration alleviated 900 MHz EMF-induced complications.

scholarly journals THE PROTECTIVE ROLE OF FLAXSEED LIGNAN IN MALE RABBITS WITH HIGH-FAT DIET: HISTOPATHOLOGICAL STUDY

2016 ◽  
Vol 8 (11) ◽  
pp. 90
Author(s):  
Zahraa Ahmed Okhti ◽  
Muthanna I. Al-ezzi ◽  
Raghad Abdulmahdi
Keyword(s):  
Blood Vessels ◽  
Bile Ducts ◽  
Mononuclear Cells ◽  
Inflammatory Cells ◽  
Negative Control Group ◽  
Negative Control ◽  
Normal Diet ◽  
Control Group ◽  
Histopathological Study ◽  
Portal Area

Objective: The objective of the study was to investigate the effect of dietary flaxseed-derived lignan supplement as anti-hyperlipidemic (fatty liver changes) on rabbit liver.Methods: Rabbits were assigned randomly into three groups (five rabbits for each group): group-1, normal diet (negative control); group-2, 1.6 % cholesterol diet for 30 d (positive control) and group-3, 1.6 % cholesterol for 30 d then gave 40 mg/kg/day of pure flax lignan for 14 d.Results: Marked proliferation of fibrous connective tissue infiltrated with mononuclear cells was recorded; congested dilated of sinusoids and fibrosis in the portal area around the proliferation of bile ducts and congested portal blood vessels with the proteinous material in the lumen of bile ducts were also recorded. In other sections, the liver showed large necrotic area replacement with RBCs as well as enlarged foamy hepatocytes with mononuclear cells in narrowing sinusoids were seen.Conclusion: From the results of this study we can conclude that lignan has been used successfully in decreasing the inflammatory cells in congested blood vessels and sinusoids and has decreased the inflammatory cells infiltration in moderate fibrosis in the portal area around the bile ducts of rabbit liver tissue.

scholarly journals Pathological study of Braken Fern ( Pteridium aquilinium ) toxicity in Rats

2013 ◽  
Vol 12 (2) ◽  
pp. 119
Author(s):  
S. O. Youkhana
Keyword(s):  
Histopathological Examination ◽  
Inflammatory Cells ◽  
Fatty Degeneration ◽  
Control Group ◽  
Albino Rats ◽  
Pathological Study ◽  
Bracken Fern ◽  
Acute Cystitis ◽  
Gross Lesions ◽  
Renal Tubular

A pathological study of bracken fern ( Pteridium aquilinium ) toxicity was conducted in rats. Twenty albino rats were randomly divided in to 4 groups. The first three groups were received diet containing 10 %, 20 % and 30 % bracken fern powder respectively. The fourth group was served as control. The experimental rats were sacrificed after one month. Gross and histopathological examination reveal lesions in many organs . Gross lesions revealed congestion of all internal organs, while the histopathological lesions were characterized by spongiosis, perivascular cuffing and astrocytosis in the brain. Swollen hepatocytes accompanied by vacuolation and fatty degeneration in the liver. Suppurative pneumonia with infiltration of inflammatory cells in the lungs. In the intestine, the villi were fused with a proliferation of cryptal enterocytes. Interstitial nephritis associated by degenerative changes in the renal tubular epithelial cells. Acute cystitis with edematous lamina propria and neutrophilic infiltration were seen in the urinary bladder. These lesions were more sever in the group 3 incomparism with group 1 and 2 . No lesions were observed in the control group

scholarly journals Effect of different doses of Tamoxifen Drug on kidney of Albino male rats: Histopathological and biochemical study

2020 ◽  
Vol 11 (3) ◽  
pp. 4590-4597
Author(s):  
Abed H. Baraaj ◽  
Aqeel H. Qasim
Keyword(s):  
Blood Vessels ◽  
Histopathological Examination ◽  
Biochemical Study ◽  
The Body ◽  
Male Rats ◽  
Control Group ◽  
Renal Tubules ◽  
Albino Rat ◽  
Vacuolar Degeneration ◽  
Histological Changes

The Tamoxifen (TAM) is an effective anticancer drug; it is a hormonal treatment against estrogen necessary for the growth of cancer cells. The study was conducted to estimate the effects of TAM on some physiological and histological parameters. We used for 40 rats of the strain albino rat [Rattus norvegicus] divided into four groups. A total of three groups were given different TAM doses (30, 40, 50mg/kg) of the body weight four times a week during 10 weeks period. The control group was injected with physiological solution (Normal Saline 0.9%). At the end of dosage duration, the blood was collected for some biochemical parameters (kidney functions) investigation. The test results showed significant differences in the treated groups as three groups revealed a significant increase in Urea, Uric Acid, Creatinine levels. The kidney tissues histopathological examination of in the TAM groups showed histological changes which increased in accordance with elevated doses administration in comparison with the control group. The histological changes were inflammatory cells infiltration, blood congestion, pyknic and necrotic nucleus in glomerulus with massive degeneration in epithelial of renal tubules, and deformed of architecture of renal tissue. In addition, massive infiltration of mononucleosis adjacent dilated blood vessels with hemorrhage in tubule and necrosis of tubules that exhibited pyknic and necrotic nucleus in glomerulus as well. As well as the exhibited small granulomatous lesion with large dilated of blood vessels and necrotic tubules with great granulomatous trauma containing from accumulation of macrophages and lymphocytes with vacuolar degeneration of tubules and massive necrotic of glomerulus with thickness of bowman capsule.

scholarly journals Histopathological Effects of Potassium Bromate on Liver Male Rat's and Possible Protective Role of Ruta chalepensis L. (Rutacae) Oil Extract

2019 ◽  
Vol 7 (2) ◽  
pp. 93-97 ◽  
Author(s):  
Ebtesam M. M. Gheth ◽  
Ibrahim S Eldurssi ◽  
Abdullah A. H. Algassi ◽  
Gasem M. A. Abdalla ◽  
Mabroka A. S. Hamad
Keyword(s):  
Blood Vessels ◽  
Inflammatory Cells ◽  
Hepatoprotective Activity ◽  
Potassium Bromate ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Bread Making ◽  
Ruta Chalepensis ◽  
Oil Extract

Potassium bromate (KBrO3) is an oxidizing agent that has been used as a food additive, mainly in the bread-making process. Ruta chalepensis L. (Family-Rutaceae) is a small shrub, native to the Mediterranean Basin. The present study aimed to investigate the protective and curative effects of R. chalepensis oil extract against KBrO3 toxicity on liver of male rats. Fifty male albino rats were divided into five groups. The first group served as a control group. The second group was administered Rue at an oral daily dose of 0.5 g/Animal for four weeks. The third group received KBrO3 100 mg/kg/b. w. for four weeks. The fourth group (protective group) was initially administered Rue alone for 2 weeks and followed by KBrO3 in association with Rue for 2 weeks. The fifth group (therapeutic group) was first given KBrO3 alone for 2 weeks and was then administered Rue in association with KBrO3 for 2 weeks. At the end of 2nd and 4th weeks of treatment, the liver tissues were dissected out for histopathological studies. Histopathological sections of rats administered with Rue showed the same histological observations as in the liver of control animals. KBrO3 treated rats exhibited marked congestion and dilatation of the blood vessels, the central veins and the portal veins. Additionally, marked infiltrative inflammatory cells were revealed. The occurrence of the cellular necrobiotic lesions and nuclei in these necrotic cells showed pyknosis. They also, showed cellular atrophied and hyaline degeneration of the cytoplasm. Vacuoles of different shapes and sizes were developed in the hepatocytes. Blood vessels being thick walled and fibrotic encircled by an inflammatory area rich in leucocytes. The protective and therapeutic groups showed marked hepatoprotective activity and better improvement than that noticed in the group which was given KBrO3 only. It may be concluded from the results that the hepatotoxic effect of KBrO3 and the ameliorative effect of Rue an effective when administrated as protective and therapeutic measures. Keywords: Rat, Liver, Potassium Bromate, Ruta chalepensis, Histopathology.

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