Antibiotic Susceptibility and Molecular Detection of Pseudomonas aeruginosa Isolated from Bovine Mastitis

2019 ◽  
Vol 43 (2) ◽  
pp. 77-85
Author(s):  
Ikram A.A. Al-Samarraae
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacterial Culture ◽  
Bovine Milk ◽  
Bovine Mastitis ◽  
Antibiotic Sensitivity ◽  
Culture Method ◽  
Resistance Rate ◽  
Good Choice ◽  
Choice Of Treatment ◽  
Antibiotic Sensitivity Test

This study aimed to isolate Pseudomonas aeruginosa from cattle (bovine) milk with mastitis to characterize its antimicrobial susceptibility against some antibiotics, and to identify aminoglycoside acetyltransferase (aac-3-Ib) gene.A total of 100 bovine milk samples were collected randomly from different local cow farms at districts of Wasit governorate, Iraq. Six P. aeruginosa isolates were obtained using bacterial culture method and further identified by Analytical Profile Index (API-20E). The antibiotic sensitivity test was performed by disc diffusion methods. Among the 5 antibiotics used, the highest resistance (100%) was found with Nalidixic acid andtetracycline, follow by gentamicin (50%) and the lowest resistance rate (16.6%, and 33.3%) was to the ciprofloxacin and cephalothin respectively. PCR was performed for all the gentamicin resistant isolates that be among the aminoglycoside family. The where the frequency of aac(3)-Ib gene have product (530bp) was to 3 of P. aeruginosa isolates. From the finding of present study we concluded that P.aeruginosa thatisolated from mastitic bovinehave been developed resistance against aminoglycosides through presence of aac(3)-Ibgene and the ciprofloxacin and cephalothin can be taken as good choice of treatment

scholarly journals Microbial Pattern and Antibiotic Sensitivity Test Among Eye Infection Patients at Bali Mandara Eye Hospital Bali

2021 ◽  
Vol 6 (2) ◽  
pp. 57-60
Author(s):  
Patria Dewi Pande
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Sensitivity ◽  
Health Care Facilities ◽  
Bacterial Conjunctivitis ◽  
Care Facilities ◽  
Antibiotic Sensitivity Test ◽  
Infection Disease ◽  
Eye Infection ◽  
Special Hospital ◽  
Keywords Infection

Abstract Infection disease of the eye usually found in health care facilities and can caused by viral,bacterial, parasite and fungi. Bali Mandara Eye Hospital was special hospital for eye disease in Bali. In this hospital infection disease of the eye dominated by bacterial conjunctivitis, viral conjunctivitis, corneal ulver, and endophtalmitis. This study was a restropective descriptive study using patient who are take eye secret culture in Bali Mandara Eye Hospital and sample were take from 2019 till 2020 It can be seen that was found seven microba and dominated disease was corneal ulcer 26 sample (86%) followed by endophtalmitis 4 sample (14%). The most microba were Pseudomonas aeruginosa. Pseudomonas aeruginosa found resistant in almost antibiotics and sensitive tolevofloxacin, gentamycin dan ceflazidime Keywords: infection, eye, culture, antibiotics

scholarly journals Pola Kepekaan Bakteri terhadap Antibiotik di Ruang Rawat Intensif RSPI Prof. Dr. Sulianti Saroso Jakarta

2017 ◽  
Vol 1 (2) ◽  
pp. 23
Author(s):  
Putriana Indah Lestari ◽  
Ika Susanti ◽  
Huda Rahmawati
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Acinetobacter Baumannii ◽  
Antibiotic Sensitivity ◽  
Secondary Data ◽  
Sensitivity Pattern ◽  
Important Health ◽  
Antibiotic Sensitivity Test ◽  
Antibiotics Sensitivity

Abstrak : Penyakit infeksi merupakan salah satu masalah kesehatan yang penting. Penggunaan antibiotik yang tidak rasional dan tepat guna pada pasien penyakit infeksi beresiko menyebabkan terjadinya resistensi. Tujuan dari penelitian ini yaitu untuk mengetahui pola kepekaan bakteri terhadap antibiotik pada pasien Ruang Rawat Intensif (ICU) RSPI Prof. Dr. Sulianti Saroso (RSPI-SS) Jakarta. Penelitian dilakukan deskriptif dan retrospektif terhadap data sekunder hasil uji kepekaan antibiotik dan jenis bakteri dari 107 pasien dalam kurun waktu 2011. Hasil menunjukkan 68 (65,4%) pasien mendapatkan hasil kultur positif dan uji kepekaan bakteri terhadap antibiotik. Jenis bakteri patogen yang dominan yaitu Acinetobacter baumannii (29,4%), disusul oleh Pseudomonas aeruginosa (27,9%), Klebsiella pneumoniae (13,2%) dan Escherichia coli (8,8%). Sebagian besar bakteri pada pasien ICU RSPISS telah berkurang kepekaannya (resisten) terhadap beberapa antibiotik. A. baumannii dan P. aeruginosa merupakan bakteri yang paling resisten terhadap antibiotik uji. Pola kepekaannya menunjukkan bahwa bakteri patogen mempunyai resistensi tertinggi terhadap erythromycin dan terendah terhadap amikasin.Infectious diseases is an important health problem. Irrational antibiotics usage is a leading cause in initiating drugs resistances. A preliminary study was conducted on the sensitivity pattern of microorganisms against antibiotics at the intensive care unit of Sulianti Infectious Diseases Hospital Jakarta. Retrospective. Secondary data were collected on the results of antibiotics sensitivity tests and species of microorganisms of 107 patients during the year 2011. Sixty eight (65,4%) patients were positive on microorganism culture test and tested on antibiotic sensitivity test. Predominance pathogenic species found were Acinetobacter baumannii (29,4%), followed by Pseudomonas aeruginosa (27,9%), Klebsiella pneumoniae (13,2%) and Escherichia coli (8,8%). Most species were less sensitive (resistant) to several antibiotics. The pattern of sensitivity showed that pathogenic microorganisms were the most resistant against erythromycin and the most sensitive antibiotics was amikacin.

Mucoid and Nonmucoid Pseudomonas aeruginosa Isolated from Respiratory Tract Infections

2016 ◽  
Vol 1 (2) ◽  
pp. 43-45
Author(s):  
Ved Prakash ◽  
Prem P Mishra
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Respiratory Tract ◽  
High Resistance ◽  
Respiratory Tract Infections ◽  
Antibiotic Sensitivity ◽  
Resistance Rate ◽  
Susceptibility Pattern ◽  
Antibiotic Susceptibility Pattern ◽  
Clinical Specimens ◽  
Tract Infections

ABSTRACT Introduction Pseudomonas aeruginosa is a common etiological agent causing various infections. The isolates can be mucoid or nonmucoid. It harbors innate resistance to a broad range of antibiotics. Objectives The aim of this study is to determine the frequency of the mucoid and nonmucoid isolates along with their antibiogram from respiratory samples. Materials and methods A total of 220 isolates of P. aeruginosa were obtained from various clinical specimens and 72 from respiratory samples. Pseudomonas aeruginosa was identified by standard colony morphology and bacteriological methods, and antibiotic sensitivity was determined by the Kirby Bauer method. Results Out of the 72 respiratory samples, 15 (20.83%) were mucoid strains and 57 (79.87%) nonmucoid strains. The mucoid colonies showed high resistance to antibiotics, such as co-trimoxazole (COT) (73.33%), ciprofloxacin (CIP) (60%), and ceftazidime (CAZ) (53.33%), whereas high resistance rate among the nonmucoid isolates were seen in (COT) (78.95%), (CIP) (71.93%), (CAZ) (54.39%), cefepime (52.63%). Conclusion The antibiotic susceptibility pattern helps in constituting the guidelines for treatment and management of the infections by P. aeruginosa. How to cite this article Mishra PP, Prakash V. Mucoid and Nonmucoid Pseudomonas aeruginosa Isolated from Respiratory Tract Infections. Int J Adv Integ Med Sci 2016;1(2):43-45.

scholarly journals POLA KUMAN PENYEBAB OTITIS EKSTERNA DAN UJI KEPEKAAN ANTIBIOTIK DI POLIKLINIK THT-KL BLU RSUP PROF. Dr. R. D. KANDOU MANADO PERIODE NOVEMBER – DESEMBER 2013

e-CliniC ◽  
2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Monica Lie Sedjati ◽  
Oraetlabora Immanuel Palandeng ◽  
Olivia Claudia Pingkan Pelealu
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Otitis Externa ◽  
Antibiotic Sensitivity ◽  
Sensitivity Test ◽  
Age Group ◽  
Chronic Stage ◽  
Antibiotic Sensitivity Test ◽  
History Of ◽  
Pattern Sensitivity

Abstract: Otitis externa is an inflammation of the ear canal that occur in acute or chronic stage caused by an infection of bacteria, viruses or fungi. The extent of the use of antimicrobials in community can lead to resistant. This study aimed to know the bacteria pattern of otitis externa and the antibiotic sensitivity test at Otorhinolaryngology Departement Prof. Dr. R. D. Kandou Hospital Manado period of November - December 2013. This is a descriptive prospective study. A total of 22 otitis externa patients, the largest age group was adults 15-49 years old with 13 persons (59,1%). Most identified gender was female by 15 people (68.2%). All the patients diagnosed with a history of trauma. The most complained symptoms were pruritus 19 people (86,3%). There were 8 types of bacteria that has been identified and the most identified were 4 samples (18,2%) as Staphylococcus aureus and 4 samples (18,2%) as Pseudomonas aeruginosa. In susceptibility test, the most sensitive antibiotic were Levofloxacin and Ciprofloxacin by 20 samples (100%). The most resistant was Clindamycin 20 sampel (100%). Conclusions : The most identified bacteria were Staphylococcus aureus and Pseudomonas aeruginosa with 4 samples (18.2%). The most sensitive antibiotic were Levofloxacin and Ciprofloxacin 20 samples (100%). Keywords: otitis externa, bacterial pattern, sensitivity test.   Abstrak: Otitis eksterna merupakan peradangan pada liang telinga yang terjadi secara akut maupun kronis yang disebabkan infeksi oleh bakteri, virus maupun jamur. Luasnya penggunaan antimikroba di kalangan masyarakat dapat berujung pada keadaan resisten. Penelitian ini bertujuan untuk mengetahui pola kuman penyebab otitis eksterna dan uji kepekaan antibiotik di poliklinik THT-KL BLU RSUP Prof. Dr. R. D. Kandou Manado periode November-Desember 2013. Penelitian ini bersifat deskriptif prospektif. Dari 22 orang pasien otitis eksterna, diperoleh kelompok umur terbanyak adalah kelompok umur dewasa 15-49 tahun sebanyak 13 orang (59,1%). Jenis kelamin terbanyak adalah perempuan berjumlah 15 orang (68,2%). Semua pasien yang terdiagnosis memiliki riwayat trauma. Gejala yang terbanyak dikeluhkan pasien otitis eksterna adalah pruritus berjumlah 19 orang (86,3%). Terdapat 8 jenis bakteri yang teridentifikasi dan terbanyak adalah Staphylococcus aureus 4 sampel (18,2%) dan Pseudomonas aeruginosa 4 sampel (18,2%). Pada uji kepekaan, antibiotik dengan sensitivitas tertinggi adalah Levofloxacin dan Ciprofloxacin 20 sampel (100%). Angka resistensi tertinggi ditunjukkan oleh Clindamycin 20 sampel (100%). Kesimpulan : Bakteri terbanyak adalah Staphylococcus aureus dan Pseudomonas aeruginosa masing -masing 4 sampel (18,2%). Angka kepekaan tertinggi ditunjukkan oleh Levofloxacin dan Ciprofloxacin 20 sampel (100%). Kata Kunci: otitis eksterna, pola kuman, uji kepekaan.

scholarly journals Detection of blaCTX-M gene among Pseudomonas aeruginosa isolated from water samples in Baghdad

2017 ◽  
Vol 28 (1) ◽  
pp. 35
Author(s):  
Saba R. Khdair
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Tap Water ◽  
Antibiotic Sensitivity ◽  
Penicillin G ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
M Gene ◽  
Antibiotic Sensitivity Test ◽  
Agar Dilution ◽  
Esbl Producers

A total of 50 environmental Pseudomonas aeruginosa isolates were collected from sewage and tap water in Baghdad, Iraq. The MICs of Cefotaxime and Ceftazidime were determined by using agar dilution method, The MIC ranged from 2 to 256 µg/ml.The results of antibiotic sensitivity test showed that among sewage P. aeruginosa isolates, resistance was observed most often to Ticarcillin (92%), Penicillin G (84%), Ceftazidime (12%), (8%) for each of Cefotaxime and Ticarcillin. On the other hand, all tap water isolates were sensitive to Ofloxacin and Levofloxacin, Except (5%) of isolates were resistant to Cefotaxime (25%) to Ceftazidime and (95%) to Ticarcillin. All isolates were tested for Extended-Spectrum β-Lactamase (ESBL) production. Ten isolates (20%) were found to be ESBL producers. All environmental P. aeruginosa isolates were screened for the presence of the blaCTX-M genes by application PCR, Only (30%) of them were positive for this test.

scholarly journals Prevalence of Pseudomonas aeruginosa and its Antibiotic Sensitivity Pattern

2019 ◽  
Vol 17 (01) ◽  
pp. 109-113 ◽  
Author(s):  
Khilasa Pokharel ◽  
Bishwa Raj Dawadi ◽  
Chandra Prakash Bhatt ◽  
Satish Gupte
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Susceptibility ◽  
Clinical Laboratory ◽  
Antibiotic Sensitivity ◽  
Tracheal Aspirate ◽  
Opportunistic Pathogen ◽  
Good Choice ◽  
Cross Sectional ◽  
Clinical Specimens ◽  
Sensitivity Pattern

Background: Pseudomonas aeruginosa is an opportunistic pathogen which causes most of the chronic infection in humans. This study was undertaken to determine the prevalence rate of Pseudomonas aeruginosa that is isolated from various clinical specimens along with its antibiotic susceptibility pattern.Methods: This descriptive cross sectional study was conducted in Kathmandu Medical College and Teaching Hospital (KMCTH) from February to May 2018. Pseudomonas aeruginosa isolated from various clinical specimens were processed in clinical laboratory, Department of Microbiology, KMCTH. Isolation, identification and sensitivity of Pseudomonas aeruginosa to antibiotics were measured.Results: A total of 7527 samples were been processed of which 46 isolates of Pseudomonas aeruginosa were obtained. Pseudomonas aeruginosa was isolated mainly from Pus, Wound swab, Sputum and Tracheal aspirate. Here 63.04% Pseudomonas aeruginosa isolates were resistant to Ceftazidime, 65.21% to Cefixime, 56.52% to Ceftriaxone and Cefotaxime followed by 56.52% to Piperacillin. Furthermore, the current study reveals antibiotics like Imipenem, Meropenem, Piperacillin/Tazobactam, Ciprofloxacin, Gentamicin, Amikacin and Tobramycin were found to be good choice for the treatment of infection caused by this organism.Conclusions: Continuous monitoring of antibiotic susceptibility pattern of Pseudomonas aeruginosa is essential and rational treatment regimens prescription by the clinicians is required to limit the spread of antimicrobial resistance.Keywords: Antibiotic resistance; clinical isolates; Pseudomonas aeruginosa.

Isolation, identification, molecular detection and sensitivity to antibiotics of Salmonella from cattle faeces

2021 ◽  
Vol 24 (1) ◽  
pp. 57-66
Author(s):  
M. N. K. Khan ◽  
M. R. Das ◽  
M. A. Sabur ◽  
M. M. Rahman ◽  
M. B. Uddin ◽  
...  
Keyword(s):  
Antibiotic Sensitivity ◽  
Culture Method ◽  
Sensitivity Study ◽  
Culture Methods ◽  
Isolation And Identification ◽  
Conventional Culture ◽  
Faecal Samples ◽  
Antibiotic Sensitivity Test ◽  
Cattle Faeces ◽  
Polymerase Chain

The present study was designed with the aim of isolation and identification of Salmonella by con-ventional culture method and their confirmation by polymerase chain reaction (PCR). Antibacte-rial sensitivity study of isolated Salmonella from cattle faeces was also performed. During the study period of July 2017 to June 2018, a total of 200 faecal samples were collected from different government and private farms in Sylhet district of Bangladesh. Out of 200 samples, 24 (12%) were found to be positive for Salmonella by conventional culture methods. Among the twenty four suspected colonies of Salmonella, seventeen were confirmed by biochemical test and same number was detected by PCR estimating a prevalence of 8.5% (17/200). The prevalence was high-er in calves under 1 year of age (16%) compared with older animals (11.25% of 1–2 years; 10% of above 2 years of age) but without statistically significant differences (χ2=4.835, P=0.089). Moreo-ver, in diarrhoeic animals the prevalence was significantly higher (32.14%, χ2=49.414, P<0.01) than in apparently healthy animals (8.72%). The antibiotic sensitivity test showed that highest number of Salmonella isolates were sensitive to ciprofloxacin (100%), gentamicin (100%) and neomycin (100%). On the other hand, significantly high resistance of Salmonella isolates was detected to erythromycin (100%), amoxicillin (100%), cotrimoxazole (81.48%), streptomycin (62.96%) followed by tetracycline (55.56%).

Isolation and Diagnosis of Pathogenic Bacteria from the Upper Surface of the Cell Phone Screen and Conduct an Antibiotic Sensitivity Test

2019 ◽  
Vol 9 (02) ◽  
Author(s):  
Muthana Badeea Farhan ◽  
Khadija Khleaf Abdulla
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cell Phone ◽  
Pathogenic Bacteria ◽  
Bacterial Culture ◽  
Culture Media ◽  
Antibiotic Sensitivity ◽  
Personal Communication ◽  
Total Sample ◽  
Final Diagnosis ◽  
Positive Bacterial Culture

A total of 120 samples (80) female samples and 40 male samples were collected from the front of the personal communication (the cell phone) for female students of Education College for Women (65) as well the medical staff of Ramadi Teaching Hospital (55), The samples are collected by swabs, The ages ranged between 19-60 years, These samples were initially culture on the blood agar medium and then on different selective and differential culture media. Api test strips were also used for the final diagnosis of bacterial isolates. The results showed that all samples gave 100% a positive result of general bacterial culture and 80% of pathogenic bacteria. The results of isolating and diagnosism indicated the presence of the pathogenic bacteria Staphylococcus aureus (58.3% of the total sample), Escherichia coli (44.2%), Enterococcus faecalis (25.8%), Pseudomonas aeruginosa (23.3%), Proteus sp. (5%), The results showed that the positive bacterial culture rate for male samples was 87.5%, whereas the female bacterial culture rate was 76.3%. The most important isolates were highly resistant to cephalexin, followed kanamycin. Proteus sp. gave the largest percentage of the resistance to 100% of those antibiotics, except for nitrofurantoin followed by Pseudomonas aeruginosa in the proportion of resistance to antibiotics used in this study.

scholarly journals Detection of qnrS and tetA resistance coding genes in Pseudomonas aeruginosaof egg-laying hens farmin Cianjur Regency, West Java

2021 ◽  
Vol 21 (2) ◽  
pp. 64-71
Author(s):  
FAUZAN ARISANDI ◽  
SAFIKA . SAFIKA ◽  
FACHRIYAN HASMI PASARIBU
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Laying Hens ◽  
Disease Incidence ◽  
Antibiotic Sensitivity ◽  
Cloacal Swab ◽  
Egg Laying ◽  
West Java ◽  
Resistance Level ◽  
Antibiotic Sensitivity Test

The use of antibiotics is an alternative to reduce disease incidence and is used to increase egg-laying hens. This study aims to detect the presence of antibiotic resistance to resistance coding genes in Pseudomonas aeruginosa bacteria. The cloacal swab samples were collected randomly as many 66 samples from several egg-laying hens farms in Cianjur Regency, West Java. The collected isolates were examined bacteriologically. The results of the antibiotic sensitivity test of 8 samples (12.1%) of the Pseudomonas aeruginosa showed the resistance level of antibiotics class tetracycline (tetracycline50%, oxytetracycline75%, and doxycycline50%), fluoroquinolone (enrofloxacin 12.5%), and phenicol (chloramphenicol 12.5%). Pseudomonas aeruginosa was resistant to tetracyclines, oxytetracyclines, doxycycline, enrofloxacin and is sensitive to chloramphenicol. The detection of resistance coding genes showed the genes amount of tetA (62.5%) and qnrS (75%). Efforts to determine the level of resistance and use of antibiotics in farms need regular surveillance and monitoring to ensure the wise use of antibiotics.

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