scholarly journals Novel topical combo-therapeutic formulation integrating honey, neomycin and bacitracin for diabetic wound management

2021 ◽  
Vol 17 (2) ◽  
pp. 167-177
Author(s):  
Emmanuel Uronnachi ◽  
Obinna Nwafor ◽  
Chinelo Ezejiegu ◽  
Josephat Obasi ◽  
Franklin Kenechukwu ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Topical Therapy ◽  
Clinical Signs ◽  
Wound Management ◽  
Diffusion Technique ◽  
Physicochemical Stability ◽  
Superior Effect ◽  
Antimicrobial Evaluation ◽  
The Individual ◽  
Diabetic Wounds

This study evaluated the healing effects of honey as a topical therapy for diabetic ulcers singly and in combination with bacitracin and neomycin (Cicatrin®), formulated as ointments in experimental rats. Antimicrobial evaluation of the test agents against Vancomycin and Oxacillin resistant Staphylococcus aureus (VORSA) and Pseudomonas aeruginosa was done by the cup plate agar-diffusion technique using the Checkerboard method. Subsequently, the optimized combination was formulated into an ointment and administered as single therapy and in combination to hyperglycemic rats made diabetic by subcutaneous injection of alloxan (130 mg/kg) and inflicted with wounds. Administration was done daily on wounds for 21 days while infected wounds had the pus from them evaluated for presence of VORSA and Pseudomonas aeruginosa. The triple combo-therapeutics formulation had improved anti-bacterial activity, in comparison with the individual formulations, with the ratio (1:9) of Cicatrin®: Honey respectively giving the best activity against VORSA. Also, the triple combo-therapeutics exhibited positive wound contraction and size reduction. Furthermore, clinical signs of infection were absent at the end of the follow-up period in the rats administered the combo-therapeutic agents while other groups of rats administered the bland ointment, and the individual agents were infected with either Pseudomonas aeruginosa or VORSA. In addition, the triple combo-therapeutics formulation exhibited good physicochemical stability throughout the treatment duration and beyond (28 days), with insignificant (p > 0.05) changes in pH and spreadability. The triple combination therapeutics formulation showed superior effect to the singly administered agents (honey and Cicatrin®) in the management of diabetic wounds

STUDYING SOME CLINICAL AND PARACLINICAL CHARACTERISTICS OF POSMATURE BABIES CARED IN NICU AT HUE UNIVERSITY HOSPITAL

2012 ◽  
pp. 74-84
Author(s):  
Thi Kieu Nhi Nguyen
Keyword(s):  
Respiratory Rate ◽  
Clinical Signs ◽  
Clinical Manifestations ◽  
University Hospital ◽  
Maternal Characteristics ◽  
Exact Test ◽  
Menstrual Cycles ◽  
Term Newborns ◽  
Student’S T ◽  
The Individual

Objectives: 1. Estimating the ratios of clinical and paraclinical signs of post-term newborns hospitalized at Pediatric Department of Hue University Hospital. 2. Identifying the relation between clinical signs and paraclinical signs. Materials and Method: 72 post- term babies < 7 days of life hospitalized at NICU from 2010/5 to 2011/4. Classification of post - term newborn was based on WHO 2003: gestational age ≥ 42 weeks with clinical manifestations: desquamation on press with fingers or natural desquamation, withered or meconial umbilicus, meconial long finger nails (*) or geatational age still < 42 weeks with theses clinical manifestations (*). Data were recorded on a clinical record form. Per-protocol analysis of clinical outcomes was performed by using Medcalc 11.5 and Excell 2007. Analyses used the χ2 test or Fisher's exact test for categorical data; Student's t test was used for continuous data and the Mann-Whitney U test for nonparametric data. Data were presented as means or proportions with 95% CIs. Results: Clinical characteristics: Tachypnea and grasp were main reasons of hospitalisation (48.61%). Poor feeding, vomitting (16.67%). Asphyxia (8.34%). Jawndice (6.94%). Hypothermia < 36.50C (13.89%), fever (13.89%). Tachypnea (59.72%). Bradycardia (1.39%). Poor feeding (11.11%). Hypertonia (9.72%). Paraclinical characteristics: Erythrocytes < 4.5.1012/l (51.39%), Leucocytes 5 – 25.103/mm3 (81.94%), Thrombocytes 100- 400.103/mm3 (94.44%). Hemoglobinemia < 10mg/l (67.61%). Maternal characteristics: Menstrual cycles regular (75%). Primiparity (75%). Amniotic volume average (70.42%), little (29.58%). Aminiotic liquid clair (62.5%), aminiotic liquid yellow (4.17%), aminiotic meconial liquid (33.33%). Maternal manifestation of one of many risk factors consist of genital infection; urinary infection; fever before, during, after 3 days of birth; prolonged delivery; medical diseases influence the foetus (75%). The relation between clinical signs and paraclinical signs: There was significantly statistical difference: between gestationnal age based on obstetrical criteria and amniotic volume on ultrasound (p < 0.05); between birth weight and glucosemia p < 0.02). There was conversional correlation of average level between erythroctes number and respiratory rate (r = - 0.5158; p < 0,0001), concordance correlation of weak level betwwen leucocytes number and respiratory rate r = 0.3045; p = 0.0093). Conclusion: It should made diagnosis of postterm baby based on degree of desquamation. The mother who has menstrual cycles regular is still delivered of a postterm baby. A postterm baby has the individual clinical and paraclinical signs.

The Perennial Problem of Variability In Adenosine Triphosphate (ATP) Tests for Hygiene Monitoring Within Healthcare Settings

2015 ◽  
Vol 36 (6) ◽  
pp. 658-663 ◽  
Author(s):  
Greg S. Whiteley ◽  
Chris Derry ◽  
Trevor Glasbey ◽  
Paul Fahey
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Staphylococcus Epidermidis ◽  
Test Point ◽  
Dilution Series ◽  
Healthcare Settings ◽  
Speed Of Response ◽  
Numerical Scale ◽  
Dilution Step ◽  
The Mean ◽  
The Individual

OBJECTIVETo investigate the reliability of commercial ATP bioluminometers and to document precision and variability measurements using known and quantitated standard materials.METHODSFour commercially branded ATP bioluminometers and their consumables were subjected to a series of controlled studies with quantitated materials in multiple repetitions of dilution series. The individual dilutions were applied directly to ATP swabs. To assess precision and reproducibility, each dilution step was tested in triplicate or quadruplicate and the RLU reading from each test point was recorded. Results across the multiple dilution series were normalized using the coefficient of variation.RESULTSThe results for pure ATP and bacterial ATP from suspensions ofStaphylococcus epidermidisandPseudomonas aeruginosaare presented graphically. The data indicate that precision and reproducibility are poor across all brands tested. Standard deviation was as high as 50% of the mean for all brands, and in the field users are not provided any indication of this level of imprecision.CONCLUSIONSThe variability of commercial ATP bioluminometers and their consumables is unacceptably high with the current technical configuration. The advantage of speed of response is undermined by instrument imprecision expressed in the numerical scale of relative light units (RLU).Infect Control Hosp Epidemiol2015;00(0):1–6

scholarly journals Biological and physicochemical stability of ceftazidime and aminophylline on glucose parenteral solution

2012 ◽  
Vol 48 (4) ◽  
pp. 691-698
Author(s):  
Carolina Alves dos Santos ◽  
Laura Oliveira-Nascimento ◽  
Marcos Camargo Knirsch ◽  
Marco Antônio Stephano ◽  
Adalberto Pessoa Júnior ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
High Performance ◽  
Inhibitory Concentration ◽  
Serum Levels ◽  
High Loss ◽  
Physicochemical Stability ◽  
Physicochemical Evaluation ◽  
The Mean ◽  
The Stability

Ceftazidime is a broad spectrum antibiotic administered mainly by the parenteral route, and it is especially effective against Pseudomonas aeruginosa. The period of time in which serum levels exceed the Minimum Inhibitory Concentration (MIC) is an important pharmacodynamic parameter for its efficacy. One of the forms to extend this period is to administer the antibiotic by continuous infusion, after prior dilution in a Parenteral Solution (PS). The present work assessed the stability of ceftazidime in 5% glucose PS for 24 hours, combined or not with aminophylline, through High Performance Liquid Chromatography (HPLC). The physicochemical evaluation was accompanied by in vitro antimicrobial activity compared MIC test in the 24-hour period. Escherichia coli and Pseudomonas aeruginosa were the microorganisms chosen for the MIC comparison. The HPLC analysis confirmed ceftazidime and aminophylline individual stability on PS, while the MIC values were slightly higher than the mean described in the literature. When both drugs were associated in the same PS, the ceftazidime concentration by HPLC decreased 25% after 24 hours. Not only did the MIC values show high loss of antibiotic activity within the same period, but also altered MIC values immediately after the preparation, which was not detected by HPLC. Our results indicate that this drug combination is not compatible, even if used right away, and that PS might not be the best vehicle for ceftazidime, emphasizing the importance of the MIC evaluation for drug interactions.

scholarly journals SELULITIS DENGAN ULKUS VARIKOSUM

2014 ◽  
Vol 6 (1) ◽  
Author(s):  
Andravita F. Mitaart ◽  
Herry E. J. Pandaleke
Keyword(s):  
Subcutaneous Tissue ◽  
Topical Therapy ◽  
Venous Blood ◽  
Clinical Signs ◽  
Lower Limbs ◽  
Body Region ◽  
Venous Blood Flow ◽  
Oral Antibiotic ◽  
Varicose Ulcer ◽  
Days Of Therapy

Abstract: Cellulitis is an acute bacterial infection of dermis and subcutaneous tissue which manifests as an erythematous lesion with an undefined border accompanied with inflammatory signs. It is mainly found in the elderly; more frequently in females than males; with a history of malaise, fever, and pain as the prodromal signs, and enlargement of local lymph nodes. Cellulitis can occur in any body region, most commonly on the lower limbs, followed by the arms, head, and, neck. It tends to occur in sites with prior lesions such as dermatitis, static ulcers (including varicose ulcers), animal bites, or trauma. A varicose ulcer is an ulcer located on the lower limb caused by a disturbance in the venous blood flow. We reported an 80-year-old woman, presented with cellulitis and varicose ulcers. The diagnosis was based on history, clinical signs, and laboratory findings. The therapy consisted of limb elevation; oral antibiotic clindamycin (300 mg), mefenamic acid (500 mg), and mebhydroline napadisilate (50 mg), each three times daily; and a topical therapy that was comprised of a wound dressing using NaCl 0.9% for 30 minutes three times daily and an application of sodium fusidate cream twice daily. After ten days of therapy, there were clinical improvements with wound healing without any sign of cellulitis.Keywords: cellulitis, varicose ulcer  Abstrak: Selulitis merupakan infeksi bakteri akut pada dermis dan jaringan subkutan yang ditandai lesi kemerahan berbatas tidak jelas dan disertai tanda-tanda radang. Umumnya selulitis ditemukan pada usia lanjut, perempuan lebih sering daripada laki-laki, dengan riwayat lesu, demam, dan rasa nyeri sebagai gejala prodromal, disertai pembesaran kelenjar getah bening setempat. Selulitis dapat terjadi pada bagian tubuh manapun dengan predileksi pada tungkai bawah diikuti lengan, kepala, dan leher. Selain itu, selulitis biasanya timbul pada lokasi dengan lesi yang telah ada sebelummya, yaitu dermatitis, ulkus stasis (termasuk ulkus varikosum), luka tusuk, gigitan binatang, atau trauma. Ulkus varikosum ialah ulkus pada tungkai bawah yang disebabkan gangguan aliran darah venosa. Kami melaporkan kasus seorang perempuan berusia 80 tahun dengan selulitis dan ulkus varikosum. Diagnosis ditegakkan berdasarkan anamnesis, gejala klinis, dan pemeriksaan penunjang. Penatalaksanaannya ialah elevasi tungkai; antibiotik oral klindamisin 300 mg, asam mefenamat 500 mg, dan mebhidrolin napadisilat 50 mg, masing-masing 3 kali sehari; kompres solusio NaCl 0,9% selama 30 menit 3 kali sehari, dan natrium fusidat krim dioleskan 2 kali sehari. Setelah 10 hari paska terapi, terdapat perbaikan klinis berupa luka yang mulai mengering tanpa disertai tanda-tanda selulitis.Kata kunci: selulitis, ulkus varikosum

Lead toxicosis in cats—a review

2003 ◽  
Vol 5 (5) ◽  
pp. 249-255 ◽  
Author(s):  
TE Knight ◽  
MSA Kumar
Keyword(s):  
Diagnostic Tests ◽  
Aminolevulinic Acid ◽  
Clinical Signs ◽  
Body Burden ◽  
Blood Lead ◽  
Good Prognosis ◽  
Metabolic Effects ◽  
Neuronal Necrosis ◽  
The Individual ◽  
High Index Of Suspicion

Although the incidence of lead toxicosis in small animals continues to decrease, it remains a significant malady. We have reviewed the literature of the past 45 years, which revealed 70 cases involving cats. Sources, signs, diagnosis, pathology and treatment of feline lead toxicosis are reviewed. In 84% of these cases the source of lead was old paint usually from home renovation. The most common signs in cats are anorexia, vomiting, and seizures. The younger individuals seem more likely to show CNS signs. Since signs are often vague, lead toxicosis may be significantly under diagnosed in cats. The gold standard of diagnostic tests is blood lead concentration, although it does not necessarily correlate with total body burden of lead or with metabolic effects including clinical signs. Diagnostic tests including erythropoietic protoporphyrin (EPP), urine aminolevulinic acid, and others are discussed. Gross findings on necropsy are few and include a yellow-brown discoloration of the liver often with a nutmeg-like appearance. Histological examination may reveal pathognomonic inclusion bodies in liver and renal tissues. Characteristic histological changes in the CNS include neuronal necrosis and demyelination. Treatment of lead toxicosis in cats, as in any species, involves removing the exposure, decontaminating the individual and the environment, supportive care and chelation therapy. The most recently available chelator is succimer (meso 2,3-dimercaptosuccinic acid). Succimer given orally is well tolerated and has a wide margin of safety. A high index of suspicion of lead toxicosis is warranted in cats since they often present with vague and non-specific signs. With any consistent history owners need to be asked about home renovation. Early diagnosis and treatment affords a good prognosis.

Keimbesiedlung von chronischen Ulcera cruris: Unterscheidet sich das diabetische vom venösen und arteriellen Geschwür?

VASA ◽  
2000 ◽  
Vol 29 (1) ◽  
pp. 62-70 ◽  
Author(s):  
Schmidt ◽  
Debus ◽  
Jeßberger ◽  
Ziegler ◽  
Thiede
Keyword(s):  
Bacterial Growth ◽  
Healing Process ◽  
Clinical Signs ◽  
The Other ◽  
Leg Ulcers ◽  
Diabetic Ulcers ◽  
Venous Ulcers ◽  
Positive Smear ◽  
Diabetic Wounds ◽  
The Impact

Background: At the Surgical Department of Surgery of the University Hospital Würzburg microbiological examinations were performed of the ulcer grounds from patients with diabetic-neuropathic, diabetic-ischemic, venous, and arterial leg ulcers. The aim of the examination was to evaluate possible differences in the healing process of these ulcers based on the knowledge of their bacterial populations. Patients and methods: In a period of four months, 63 patients were consecutively examined by taking a bacteriological swab of their ulcer area. The healing process of their wounds was followed and related to the impact of bacterial colonisation and clinical signs of infection. Results: 95% of the venous and arterial leg ulcers had a positive smear, whereas only 70% of diabetic ulcers were positive for bacterial growth. Bacterial population of the three ulcer entities, however did not differ significantly. 100% of the clinically infected venous and arterial ulcers but only 80% of the diabetic wounds revealed a positive smear. On the other hand, only 22% of the venous ulcers with a positive smear developed a clinical infection in contrast to 70% of the arterial and diabetic. Venous ulcers showed only in a few patients prolonged healing, even in cases of marked bacterial contamination. Despite of clinical signs of infection however, diabetic wounds sometimes did not reveal a positive wound smear (20%). All infected venous, but only 20% of the infected ischemic ulcers healed satisfactorily. Arterial wounds with no bacterial growth healed significantly better than contaminated wounds. This difference was not significant in the other entities. Radical removal of the infection by minor amputation increased the healing rate in diabetic ulcers over 80%, whereas ischemic wounds did not profit from this therapy. Conclusions: A positive bacterial wound smear is not inevitably correlated with a protracted leg ulcer healing. Nevertheless a fulminant infection often developed in diabetic ulcers despite the initial inability to demonstrate bacterial growth. In order to start antibiotic treatment as early as possible, a wound smear should be obtained routinely from patients with diabetic ulcers. In chronic venous ulcers, a routine swab does not appear to be indicated as it bears no clinical consequences. The same applies to patients with surgically fully treated peripheral arterial occlusive disease. As ischemia presents the limiting factor, antibiotic therapy in case of infection will not prevent imminent amputation.

scholarly journals Help, hinder, hide and harm: what can we learn from the interactions between Pseudomonas aeruginosa and Staphylococcus aureus during respiratory infections?

Thorax ◽  
2019 ◽  
Vol 74 (7) ◽  
pp. 684-692 ◽  
Author(s):  
Dominique Hope Limoli ◽  
Lucas R Hoffman
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Respiratory Infections ◽  
Clinical Importance ◽  
Community Members ◽  
Culture Independent ◽  
State Of Research ◽  
The Individual ◽  
Lung Health ◽  
Respiratory Microbiota

Recent studies of human respiratory secretions using culture-independent techniques have found a surprisingly diverse array of microbes. Interactions among these community members can profoundly impact microbial survival, persistence and antibiotic susceptibility and, consequently, disease progression. Studies of polymicrobial interactions in the human microbiota have shown that the taxonomic and structural compositions, and resulting behaviours, of microbial communities differ substantially from those of the individual constituent species and in ways of clinical importance. These studies primarily involved oral and gastrointestinal microbiomes. While the field of polymicrobial respiratory disease is relatively young, early findings suggest that respiratory tract microbiota members also compete and cooperate in ways that may influence disease outcomes. Ongoing efforts therefore focus on how these findings can inform more ‘enlightened’, rational approaches to combat respiratory infections. Among the most common respiratory diseases involving polymicrobial infections are cystic fibrosis (CF), non-CF bronchiectasis, COPD and ventilator-associated pneumonia. While respiratory microbiota can be diverse, two of the most common and best-studied members are Staphylococcus aureus and Pseudomonas aeruginosa, which exhibit a range of competitive and cooperative interactions. Here, we review the state of research on pulmonary coinfection with these pathogens, including their prevalence, combined and independent associations with patient outcomes, and mechanisms of those interactions that could influence lung health. Because P. aeruginosa–S. aureus coinfection is common and well studied in CF, this disease serves as the paradigm for our discussions on these two organisms and inform our recommendations for future studies of polymicrobial interactions in pulmonary disease.

scholarly journals Calpurnia aurea (Aiton) Benth Extracts Reduce Quorum Sensing Controlled Virulence Factors in Pseudomonas aeruginosa

Molecules ◽  
2020 ◽  
Vol 25 (10) ◽  
pp. 2283
Author(s):  
Sekelwa Cosa ◽  
Jostina R. Rakoma ◽  
Abdullahi A. Yusuf ◽  
Thilivhali E. Tshikalange
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Molecular Docking ◽  
Quorum Sensing ◽  
Virulence Factors ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Promising Alternative ◽  
The Individual

Pseudomonas aeruginosa is the causative agent of several life-threatening human infections. Like many other pathogens, P. aeruginosa exhibits quorum sensing (QS) controlled virulence factors such as biofilm during disease progression, complicating treatment with conventional antibiotics. Thus, impeding the pathogen’s QS circuit appears as a promising alternative strategy to overcome pseudomonas infections. In the present study, Calpurnia aurea were evaluated for their antibacterial (minimum inhibitory concentrations (MIC)), anti-quorum sensing/antivirulence (AQS), and antibiofilm potential against P. aeruginosa. AQS and antivirulence (biofilm formation, swimming, and swarming motility) activities of plant extracts were evaluated against Chromobacterium violaceum and P. aeruginosa, respectively. The in vitro AQS potential of the individual compounds were validated using in silico molecular docking. Acetone and ethanolic extracts of C. aurea showed MIC at 1.56 mg/mL. The quantitative violacein inhibition (AQS) assay showed ethyl acetate extracts as the most potent at a concentration of 1 mg/mL. GCMS analysis of C. aurea revealed 17 compounds; four (pentadecanol, dimethyl terephthalate, terephthalic acid, and methyl mannose) showed potential AQS through molecular docking against the CviR protein of C. violaceum. Biofilm of P. aeruginosa was significantly inhibited by ≥60% using 1-mg/mL extract of C. aurea. Confocal laser scanning microscopy correlated the findings of crystal violet assay with the extracts significantly altering the swimming motility. C. aurea extracts reduced the virulence of pseudomonas, albeit in a strain- and extract-specific manner, showing their suitability for the identification of lead compounds with QS inhibitory potential for the control of P. aeruginosa infections.

scholarly journals Studies of Biologically Active Heterocycles: Synthesis, Characterization and Antimicrobial Activity of Some 2,5-Disubstituted-1,3,4-Oxadiazoles

1970 ◽  
Vol 6 (2) ◽  
pp. 69-75 ◽  
Author(s):  
G Nagalakshmi
Keyword(s):  
Phosphorus Oxychloride ◽  
Biologically Active ◽  
Shigella Dysenteriae ◽  
Diffusion Method ◽  
Diffusion Technique ◽  
Antimicrobial Evaluation ◽  
Bacteria And Fungi ◽  
Different Strains ◽  
Agar Well Diffusion Method

1,3,4-oxadiazoles are important because of its versatile biological actions. In the present study, several 2,5-disubstituted-1,3,4-oxadiazoles (3a-o) have been synthesized by the condensation of 4-hydroxybenzohydrazide (1) with various aromatic acids (2a-o) in presence of phosphorus oxychloride. The structures of the newly synthesized compounds have been established on the basis of elemental analysis, UV, IR and 1H NMR spectral data. The synthesized compounds were screened for their in vitro growth inhibiting activity against different strains of bacteria and fungi viz., Staphylococcus aureus, Bacillus subtilis, Bacillus megaterium, Escherichia coli, Pseudomonas aeruginosa, Shigella dysenteriae, Candida albicans, Aspergillus niger and Aspergillus flavus and the results were compared with the standard antibiotics such as chloramphenicol (50?g/ml) and Griseofulvin (50?g/ml) using agar diffusion technique. Compounds 3b, 3e, 3g, 3h, 3j, 3m and 3n exhibited strong antibacterial activity and compounds 3a, 3d, 3g, 3h and 3i showed good antifungal activity. Key words: 2,5-disubstituted-1,3,4-oxadiazoles, 4-hydroxybenzohydrazide, phosphorus oxychloride, antimicrobial evaluation, agar well diffusion method. Dhaka Univ. J. Pharm. Sci. 6(2): 69-75, 2007 (December)

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