scholarly journals BAY LEAF (SYZYGIUM POLYANTHUM) EXTRACT GEL EFFECT ON TNF- Α EXPRESSION IN TRAUMATIC ULCERS HEALING PROCESS

2020 ◽  
Vol 7 (1) ◽  
pp. 25
Author(s):  
Annisha Savira ◽  
Rochman Mujayanto ◽  
Musri Amurwaningsih
Keyword(s):  
Healing Process ◽  
Gel Effect ◽  
Tnf Α

scholarly journals An Engineered Infected Epidermis Model for In Vitro Study of the Skin’s Pro-Inflammatory Response

Micromachines ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 227 ◽  
Author(s):  
Maryam Jahanshahi ◽  
David Hamdi ◽  
Brent Godau ◽  
Ehsan Samiei ◽  
Carla Sanchez-Lafuente ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Inflammatory Response ◽  
Human Skin ◽  
Electrical Resistance ◽  
Drug Testing ◽  
Healing Process ◽  
Tnf Α ◽  
Hydrogel Matrix ◽  
Significant Difference

Wound infection is a major clinical challenge that can significantly delay the healing process, can create pain, and requires prolonged hospital stays. Pre-clinical research to evaluate new drugs normally involves animals. However, ethical concerns, cost, and the challenges associated with interspecies variation remain major obstacles. Tissue engineering enables the development of in vitro human skin models for drug testing. However, existing engineered skin models are representative of healthy human skin and its normal functions. This paper presents a functional infected epidermis model that consists of a multilayer epidermis structure formed at an air-liquid interface on a hydrogel matrix and a three-dimensionally (3D) printed vascular-like network. The function of the engineered epidermis is evaluated by the expression of the terminal differentiation marker, filaggrin, and the barrier function of the epidermis model using the electrical resistance and permeability across the epidermal layer. The results showed that the multilayer structure enhances the electrical resistance by 40% and decreased the drug permeation by 16.9% in the epidermis model compared to the monolayer cell culture on gelatin. We infect the model with Escherichia coli to study the inflammatory response of keratinocytes by measuring the expression level of pro-inflammatory cytokines (interleukin 1 beta and tumor necrosis factor alpha). After 24 h of exposure to Escherichia coli, the level of IL-1β and TNF-α in control samples were 125 ± 78 and 920 ± 187 pg/mL respectively, while in infected samples, they were 1429 ± 101 and 2155.5 ± 279 pg/mL respectively. However, in ciprofloxacin-treated samples the levels of IL-1β and TNF-α without significant difference with respect to the control reached to 246 ± 87 and 1141.5 ± 97 pg/mL respectively. The robust fabrication procedure and functionality of this model suggest that the model has great potential for modeling wound infections and drug testing.

Exercise accelerates cutaneous wound healing and decreases wound inflammation in aged mice

2008 ◽  
Vol 294 (1) ◽  
pp. R179-R184 ◽  
Author(s):  
K. Todd Keylock ◽  
Victoria J. Vieira ◽  
Matthew A. Wallig ◽  
Luisa A. DiPietro ◽  
Megan Schrementi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Moderate Intensity ◽  
Wound Healing Process ◽  
Indirect Measure ◽  
Monocyte Chemoattractant Protein 1 ◽  
Aged Mice ◽  
Wound Size ◽  
Tnf Α ◽  
Old Mice

The purpose of this study was to determine the effect of exercise on wound healing and inflammation in young (3 mo) and old (18 mo) female BALB/cByJ mice. Mice were assigned to either exercise or sedentary control (control) groups. The exercise group mice were run on a motorized treadmill at a moderate intensity 30 min/day for 8 days. All mice were given four full-thickness dermal wounds, and the rate of wound closure was assessed daily for 10 days. Four months later, the aged mice were rerandomized to treatment, wounded again in different locations, and wounds were harvested at 1, 3, or 5 days postwounding. Wound tissue was analyzed for IL-1β, IL-6, keratinocyte chemoattractant (KC), monocyte chemoattractant protein-1 (MCP-1), and TNF-α protein. Myeloperoxidase (MPO) activity and F4/80 mRNA were assessed as an indirect measure of neutrophil and macrophage content, respectively. There was a trend ( P = 0.10) for exercise to reduce wound size in young mice, and exercise significantly ( P < 0.05) decreased wound size in old mice. TNF-α, KC, and MCP-1 were significantly ( P < 0.05) lower in wounds from exercised old mice compared with control. No group differences were found for wound IL-1β or IL-6, MPO activity, or F4/80 mRNA. Our data suggest that exercise accelerates the wound healing process in old mice. This improved healing response in the old mice may be the result of an exercise-induced anti-inflammatory response in the wound.

scholarly journals Corneal Healing and Recovery of Ocular Crystallinity with a Dichloromethane Extract of Sedum dendroideum D.C. in a Novel Murine Model of Ocular Pterygium

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4502
Author(s):  
Luiselva Torrescano-De Labra ◽  
Enrique Jiménez-Ferrer ◽  
Brenda Hildeliza Camacho-Díaz ◽  
Gabriela Vargas-Villa ◽  
Manases González-Cortazar ◽  
...  
Keyword(s):  
Phorbol Esters ◽  
Healing Process ◽  
Corneal Scarring ◽  
Tetradecanoylphorbol Acetate ◽  
Ophthalmic Administration ◽  
Tnf Α ◽  
Healthy Control ◽  
Corneal Damage

Pterygium is a corneal alteration that can cause visual impairment, which has been traditionally treated with the sap of Sedum dendroideum D.C. The pharmacological effect of a dichloromethane extract of S. dendroideum was demonstrated and implemented in a pterygium model on the healing process of corneal damage caused by phorbol esters. In mice of the ICR strain, a corneal lesion was caused by intravitreal injection of tetradecanoylphorbol acetate (TPA). The evolution of the corneal scarring process was monitored with vehicle, dexamethasone, and dichloromethane extract of S. dendroideum treatments by daily ophthalmic administration for fifteen days. The lesions were evaluated in situ with highlighted images of fluorescence of the lesions. Following treatment levels in eyeballs of IL-1α, TNF-α, and IL-10 cytokines were measured. The effective dose of TPA to produce a pterygium-like lesion was determined. The follow-up of the evolution of the scarring process allowed us to define that the treatment with S. dendroideum improved the experimental pterygium and had an immunomodulatory effect by decreasing TNF-α, IL-1α, and maintaining the level of IL-10 expression, without difference with respect to the healthy control. Traditional medical use of S. dendroideum sap to treat pterygium is fully justified by its compound composition.

scholarly journals The Impairment of Wound Healing Process is Correlated With Abnormalities of TNF-α Production by Peritoneal Exudate Cells in Obstructive Jaundiced Rats

HPB Surgery ◽  
2000 ◽  
Vol 11 (5) ◽  
pp. 311-318 ◽  
Author(s):  
Janusz Dawiskiba ◽  
Danuta Kwiatkowska ◽  
Michał Zimecki ◽  
Pawel Kornafel ◽  
Wanda Tyran ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Endothelial Cell ◽  
Breaking Strength ◽  
Healing Process ◽  
Skin Wound ◽  
Endothelial Cell Proliferation ◽  
Wound Healing Process ◽  
Hydroxyproline Content ◽  
Tnf Α

The wound healing process and production of tumour necrosis factor alpha (TNF-α) by peritoneal cells of 7-day and 14-day obstructive jaundice (OJ) and sham-operated rats were investigated. In the study the skin wound breaking strength was measured, In addition such histological and biochemical parameters as fibroblast and endothelial cell proliferation, inflammatory cell infiltration and hydroxyproline content were evaluated in polyurethane sponge discs implanted subcutaneously into rats. TNF-α production by peritoneal exudate cells (PEC), both spontaneous and lipopolysaccharide (LPS)- induced was determined by a bioassay. In OJ rats the process of both early as well as late phase of healing was impaired. The breaking strength of skin wound was decreased, the fibroblast and endothelial cell proliferation and collagen deposition, as well as hydroxyproline content were diminished. In 7 day OJ the numbers of inflammatory cells in the implants were lowered with a subsequent slight increase on day 14 of OJ. The spontaneous and LPS induced TNF- α production by PEC were significantly higher in 7 day OJ as compared with sham-operated controls. On day 14 of OJ the LPS-induced TNF-α level was, in contrast, much lower and did not differ much from the spontaneous TNF-α production. We conclude that the impairment of wound healing in OJ results from disturbances in functioning of the immune system caused by systemic endotoxaemia.

scholarly journals Burn Ointment Promoted Cutaneous Wound for Burns and Scalds by Modulating the PI3K/AKT/mTOR Signaling Pathway

2020 ◽  
Author(s):  
Da li Gan ◽  
Yan Yao ◽  
Qi yuan Su ◽  
Su qin Yang ◽  
Li Wu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Skin Irritation ◽  
Mtor Pathway ◽  
Epithelial Tissue ◽  
Burn Wounds ◽  
Degree Burn ◽  
Tnf Α ◽  
Tgf Β1 ◽  
Second Degree

Abstract Background: Burn injury is common, burn ointment (BO) is a common preparation used to treat burns and scalds in folk as an effective remedy for burn healing. The present study was undertaken to evaluate the healing effect and related underlying mechanisms of BO in a model of deep second-degree thermal burn by animal experiments. Methods: BO is was made up of a combination of extracts from several traditional Chinese medicine and borneol, solid paraffin, rapeseed oil, and and its quality control was assessed. The acute toxicity test and skin irritation test were evaluated by rats. The anti-inflammatory effect was revealed by using inflammation animal models, including the xylene-induced auricle swelling in mice and carrageenan-induced toe swelling in rats. The hot plate test was used to evaluate its analgesic activity. Moreover, the experiments of knife and a deep second-degree burn wound were used to explore the effect of BO in promoting wound healing. On days 7, 14 and 28, the wounds were digitally photographed by a camera and after sacrifice of the SD rats, skin samples were obtained for performing H&E staining, immunohistochemical staining and Western Blotting examination. In addition, The expressed of TNF-α, TGF-β1 and VEGF in serum were detected by ELISA kits. Results: BO had no toxicity or side effects on the skin and liver or kidney function. BO could significantly inhibit auricular swelling in mice, paw welling in rats and markedly prolonged the latencies of licking paws in mice; it also could accelerate the process of wound healing and repair scar by promoting the formation of new epithelial tissue. In addition, BO significantly reduced the content of TNF-α and markedly increased the content of VEGF and TGF-β1 in the serum. Moreover, BO promoted the expression of collagen I. Furthermore, it increased the ratio of p-PI3K/PI3K, p-AKT/AKT and p-mTOR/mTOR in the PI3K / AKT / mTOR pathway.Conclusions: BO could effectively reduce inflammation and pain, and effectively accelerate the healing process of deep second-degree burn wounds. And the mechanism of BO promoting wound healing may be related to activate PI3K / AKT / mTOR pathway. therefore, it may be recommended as a promising topical medication for treating burn wounds in the future clinical trials.

Restitution of single-cell defects in the mouse colon epithelium differs from that of cultured cells

2006 ◽  
Vol 290 (6) ◽  
pp. R1496-R1507 ◽  
Author(s):  
D. Günzel ◽  
P. Florian ◽  
J. F. Richter ◽  
H. Troeger ◽  
J. D. Schulzke ◽  
...  
Keyword(s):  
Single Cell ◽  
Laser Scanning ◽  
Pathogenic Bacteria ◽  
Cultured Cells ◽  
Healing Process ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Rapid Repair ◽  
E Coli ◽  
Tnf Α

Integrity of colon epithelium is of crucial importance and, as small defects occur constantly, rapid repair (restitution) is essential. To investigate the mechanism of restitution, single-cell lesions were induced in mouse colonic surface epithelia by iontophoretic injection of Ca2+. Closure of the resulting defects was monitored using confocal laser scanning microscopy (CLSM), and functional sealing by electrophysiological techniques. Restitution was evaluated as the time constant τ of the exponential decrease in conductance of an induced leak and amounted to 0.28 min under control conditions. After 4 min, the leak was completely sealed. Repair was thus considerably faster than in previously investigated HT-29/B6 cells (τ = 5.73 min). As in cultured cells, cytochalasin D delayed restitution in native colon epithelia (τ = 0.69 min), indicating the involvement of actin in the healing process; however, no accumulation of actin surrounding the lesion was detected. Long-term incubation of epithelia with IFN-γ alone or in combination with TNF-α increased τ to 0.49 and 0.59 min, respectively. In contrast to cultured cells, TNF-α alone did not affect restitution. A brief (<10 min) exposure to the sterile filtered supernatant of hemolytic E. coli O4 cultures did not affect the morphology of the epithelium, but delayed restitution. In CLSM studies, defects were still clearly visible 4 min after the onset of lesion induction. The supernatant of a nonhemolytic E. coli O4 mutant did not exhibit this effect. In conclusion, single-cell defects in native colon cause functional leaks that seal faster than in cell cultures. Proinflammatory cytokines and pathogenic bacteria delay restitution. This suggests a key role of very small lesions at the onset of pathogenic processes in the intestine.

scholarly journals Multifunctional, TNF-α and IFN-γ-Secreting CD4 and CD8 T Cells and CD8High T Cells Are Associated With the Cure of Human Visceral Leishmaniasis

2021 ◽  
Vol 12 ◽  
Author(s):  
Lorranny Santana Rodrigues ◽  
Aline Silva Barreto ◽  
Lays Gisele Santos Bomfim ◽  
Marcos Couto Gomes ◽  
Nathalia Luisa Carlos Ferreira ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Visceral Leishmaniasis ◽  
Mononuclear Cells ◽  
Healing Process ◽  
T Cell Response ◽  
Peripheral Blood Mononuclear ◽  
Tnf Α ◽  
Ifn Γ

Visceral leishmaniasis (VL) is a chronic and often fatal disease caused by protozoans of the genus Leishmania that affects millions of people worldwide. Patients with symptomatic VL have an impaired anti-Leishmania-specific CD4+ T-cell response, which is reversed after clinical cure. In contrast, the quality of the CD4+ and CD8+ T-cell responses involved in resistance and/or cure of VL relies on the capability of these cells to activate polyfunctional and memory responses, which are associated with the simultaneous production of three cytokines: IFN-γ, IL-2, and TNF-α. Models for the development of CD4 and CD8 T-cell quality in memory and protection to leishmaniasis have been described previously. We aimed to assess the functionality of the T cells involved in the recovery of the immune suppression throughout the VL treatment. Therefore, we cultured peripheral blood mononuclear cells (PBMCs) from VL patients and healthy controls in vitro with soluble Leishmania antigen (SLA). Cell surface markers and intracellular cytokine production were determined on days 7, 14, 21, 30, 60, 90, and 180 after the beginning of chemotherapy. We observed that the frequencies of CD4+TNF-α+IFN-γ+ and the multifunctional CD4+IL-2+TNF-α+IFN-γ+, together with CD4+TNF-α+ and CD4+IFN-γ+ T cells, increased throughout and at the end of the treatment, respectively. In addition, enhanced frequencies of CD8+IL-2+TNF-α+IFN-γ+ and CD8+TNF-α+IFN-γ T cells were also relevant in the healing process. Noteworthy, the frequencies of the CD4+ and CD8 central-memory T cells, which produce IL-2, TNF-α, and IFN-γ and ensure the memory response against parasite reinfection, are significantly enhanced in cured patients. In addition, the subset of the non-functional CD8Low population is predominant in VL untreated patients and decreases along the chemotherapy treatment. In contrast, a CD8High subset increased towards the cure. Furthermore, the cure due to treatment with meglumine antimoniate or with liposomal amphotericin B was associated with the recovery of the T-cell immune responses. We described the evolution and participation of functional T cells during the treatment of patients with VL. Our results disclosed that the clinical improvement of patients is significantly associated with the participation of the CD4+ and CD8+ cytokine-secreting T cells.

scholarly journals PENGARUH PEMBERIAN TOPIKAL EKSTRAK KOLAGEN KULIT IKAN LELE SANGKURIANG (CLARIAS GARIEPINUS VAR) TERHADAP TNF-Α DAN JUMLAH FIBROBLAST PADA LUKA BAKAR DERAJAT DUA TIKUS WISTAR

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Aisyah . ◽  
Zainatul Mufarikoh ◽  
Ary Andini
Keyword(s):  
Treatment Group ◽  
Healing Process ◽  
Clarias Gariepinus ◽  
Burn Injuries ◽  
Control Group ◽  
Level Ii ◽  
Tnf Α ◽  
Significant Difference ◽  
Group 2 ◽  
Group 1

ABSTRACTBackground: Collagen has vital role on healing process of burn injuries, mainly in connective tissue.Collagen triggers fibroblast proliferation, support to form new granulation tissue and epithelium aroundwound. Wound treatment through collagen extract of Sangkuriang catfish skin can increase healing process.It stimulates humidity on level II burn injuries and encourage re-epithelization, proliferation and cellmigration also increases growth. Methods: This is an experimental studies using 4 groups of RattusNorvegicus (Wistar strain). All of the group get a burn injuries in their back skin. Control group 1 (K1) wastreated by lidocaine for three days and control group 2 (K2) for ten days. Whereas treatment group 1 (P1) getlidocaine and collagen extract from Sangkuriang cat fish for 3 days and treatment group 2 (P2) for 10 days.Result: Number of fibroblast/field of view on treatment group showed significantly increased comparedcontrol groups on the 3rd days (P=0,046) and the 10th (P=0,004). Percentage of TNF-α on level II-burninjuries in wistar rats showed significant difference. Percentage of TNF-α lower than treatment group andshowed significant lowering level compared control groups on the10th (P=0,022). Conclusion: Increasing offiroblast number and lowering level of TNF-α significantly showed collagen extract of Sangkuriang cat fishskin accelerate wound healing process.

scholarly journals Diabetic Wound Healing Biosurfactants Dialkyl Alginate Cream on TNF-α TGF-β Expression, Reepithelization, and Collagenization

2019 ◽  
Vol 17 (1) ◽  
pp. 72
Author(s):  
Cut Raihanah ◽  
Nurul Mahyani ◽  
Kintoko Kintoko
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Wound Healing Process ◽  
Epithelial Tissue ◽  
Significant Activity ◽  
Diabetic Wound ◽  
Tnf Α ◽  
Healing Agent ◽  
Diabetic Wound Healing ◽  
Made In

Diabetic wound healing is delayed by many factors, including high TNF-α expression and low TGF-β expression which can affect the formation of new epithelial tissue and collagen as the main goal of the wound healing process. One of the diabetic wound healing agent is biosurfactant dialkyl alginate where so far its use in cream form for diabetic wound has never been reported. This study aimed to determine TNF-α, TGF-β, reepithelization and the collagenization of biosurfactant dialkyl alginate cream in diabetic biopsy wounds in STZ-induced rat. Biosurfactant dialkyl alginate was made in cream form and applied to biopsy wounds on the backs of rat twice a day for 9 days. Observation of TNF-α and TGF-β expression were performed by immunohistochemical staining, while epithelial and collagen with staining HE and Mallory. The results showed that the biosurfactant dialkyl alginate cream had an activity to decrease TNF-α expression, increase TGF-β expression and reepithelization but did not have any significant activity on collagenization. These results suggest that the biosurfactant dialkyl alginate cream can accelerate the healing of diabetic wound.

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