scholarly journals Antinociceptive and cytotoxic potential of ethanolic extract of Delonix regia (Leaves)

2014 ◽  
Vol 2 (01) ◽  
pp. 55-61
Author(s):  
Rozina Parul ◽  
Md. Jahir Alam ◽  
Md. Sohel Rana
Keyword(s):  
Cytotoxic Activity ◽  
Formalin Test ◽  
Antinociceptive Effect ◽  
Ethanolic Extract ◽  
Alcoholic Extract ◽  
Cytotoxic Potential ◽  
Cytotoxic Effects ◽  
Standard Drug ◽  
Delonix Regia

This study investigated the antinociceptive and cytotoxic effects of alcoholic extract of Delonix regia Leaves in rodents. Antinociceptive activities were done using paw licking and writhing tests. The extract was used at dose of 200 and 400 mg/kg per orally as compared to Standard drug Diclofenac-Na 100 mg/kg bw in Swiss albino mice of body weight 25-30 g. The cytotoxic activity was determined by the Brine Shrimp lethality bioassay. The alcoholic extract showed very highly significant effect in formalin test (p 0.01). Also had the highest analgesia (p 0.001) in the acetic acid-induced writhing model as well. The extract possessed a good in vitro cytotoxic activity (LC50=4.06 μg/ml). The results suggested that Delonix regia Leaves extract has a suitable antinociceptive effect as well as cytotoxic potential.

Evaluation of Hepatoprotective and In-vitro Cytotoxic Activity of Leaves of Premna serratifolia Linn

2008 ◽  
Vol 1 (1) ◽  
pp. 145-152 ◽  
Author(s):  
Rajendran Vadivu ◽  
A. Jerad Suresh ◽  
K Girinath ◽  
P. Boopathi Kannan ◽  
R Vimala ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Hepatoprotective Activity ◽  
Alcoholic Extract ◽  
Standard Drug ◽  
Degree Of Protection ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Traditional Practitioners ◽  
Serum Glutamate

Premna serratifolia is used by the traditional practitioners as cardiotonic, antibiotic, anti-coagulant, stomachic, carminative, hepatoprotective, antitumor etc. The present study aims in the evaluation of hepatoprotective and in-vitro cytotoxic activity of alcoholic extract of leaves of Premna serratifolia Linn. Hepatoprotective activity is studied by carbon tetrachloride induced hepato-toxicity in rats and the in-vitro cytotoxic activity is carried out by tryphane blue exclusion method using EAC cell lines. The degree of protection in hepatoprotective activity has been measured by using biochemical parameters such as serum glutamate oxalate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), bilirubin and total protein. The results suggest that the alcoholic extract at the dose level of 250mg/kg has produced significant  (p < 0.001) hepatoprotection by decreasing the activity of serum enzymes, bilirubin, and lipid peroxidation which is comparable to that of standard drug silymarin. The alcoholic extract also does exhibit the IC50 value of 75µg/ml which indicates the significant in-vitro cytotoxic activity of the extract. It is concluded that alcoholic extract of leaves of Premna serratifolia Linn is not only an effective hepatoprotective agent, but also possesses significant antitumor activity.  Keywords: Premna serratifolia Linn; Alcoholic extract hepatoprotective; In-vitro cytotoxic activity.  © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v1i1.1046 

Design, Synthesis, In vitro Cytotoxic Activity Evaluation, and Study of Apoptosis Inducing Effect of New Styrylimidazo[1,2-a]Pyridines as Potent Anti-Breast Cancer Agents

2019 ◽  
Vol 19 (2) ◽  
pp. 265-275 ◽  
Author(s):  
Faeze Khalili ◽  
Sara Akrami ◽  
Malihe Safavi ◽  
Maryam Mohammadi-Khanaposhtani ◽  
Mina Saeedi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
High Yield ◽  
Breast Cancer Cell Lines ◽  
Pyridine Derivatives ◽  
One Pot ◽  
Standard Drug ◽  
Three Component Reaction ◽  
Anti Cancer

Background: This paper reports synthesis, cytotoxic activity, and apoptosis inducing effect of a novel series of styrylimidazo[1,2-a]pyridine derivatives. Objective: In this study, anti-cancer activity of novel styrylimidazo[1,2-a]pyridines was evaluated. Methods: Styrylimidazo[1,2-a]pyridine derivatives 4a-o were synthesized through a one-pot three-component reaction of 2-aminopyridines, cinnamaldehydes, and isocyanides in high yield. All synthesized compounds 4a-o were evaluated against breast cancer cell lines including MDA-MB-231, MCF-7, and T-47D using MTT assay. Apoptosis was evaluated by acridine orange/ethidium bromide staining, cell cycle analysis, and TUNEL assay as the mechanism of cell death. Results: Most of the synthesized compounds exhibited more potent cytotoxicity than standard drug, etoposide. Induction of apoptosis by the most cytotoxic compounds 4f, 4g, 4j, 4n, and 4m was confirmed through mentioned methods. Conclusion: In conclusion, these results confirmed the potency of styrylimidazo[1,2-a]pyridines for further drug discovery developments in the field of anti-cancer agents.

scholarly journals The cytotoxic activity of pine needles ethanolic extract of Pinus merkusii on HeLa cell lines

2021 ◽  
Vol 33 ◽  
pp. 03001
Author(s):  
Annise Proboningrat ◽  
Amaq Fadholly ◽  
Sri Agus Sudjarwo ◽  
Fedik Abdul Rantam ◽  
Agung Budianto Achmad
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Anticancer Agents ◽  
Anticancer Agent ◽  
Ethanolic Extract ◽  
In Vitro Cytotoxicity ◽  
Hela Cell Lines ◽  
Cytotoxicity Assessment ◽  
Pinus Merkusii

Several efforts have been made to discover new anticancer agents based on natural ingredients. Meanwhile, previous studies have shown that different Pine genus species exhibit cytotoxic activity against various types of cancer cells. This plant is rich in phenolic compounds, especially procyanidins, flavonoids, and phenolic acids. Therefore, this study aims to investigate the in vitro cytotoxicity of Pinus merkusii needles extract on HeLa cancer cell lines. The cytotoxicity assessment was measured using MTT assay and expressed as IC50 value. The results showed that the ethanolic extract poses a dose and time-dependent cytotoxic activity with an IC50 value of 542.5 µg/ml at 48 hours of incubation. Based on this result, Pinus merkusii needles’ ethanolic extract has the potential of a novel candidate for an anticancer agent.

Cytotoxic Activity of tropane alkaloides of Species of Erythroxylum

2021 ◽  
Vol 21 ◽  
Author(s):  
José Fernando Araújo Neto ◽  
Erika Maria de Oliveira Ribeiro ◽  
Ademir Evangelista do Vale ◽  
Jorge Maurício David ◽  
Juceni Pereira de Lima David
Keyword(s):  
Natural Products ◽  
Cytotoxic Activity ◽  
Screening Program ◽  
Evaluation Studies ◽  
Tropane Alkaloids ◽  
Chemotherapeutic Agents ◽  
In Vitro Tests ◽  
Cytotoxic Activities ◽  
Cytotoxic Potential

: Erythroxylaceae is a family composed of four genera being Erythroxylum the only one represented in the Neotropical region. Chemical studies indicate the presence of alkaloids, terpenes, flavonoids and phenolic compounds as main compounds. The incorporation of cytotoxic activity assays of natural products using cell cultures assists in the selection of potential chemotherapeutic agents. In this work we describe a revision of the cytotoxicity evaluation studies performed with extracts and/or pure substances obtained from Erythroxylum species through an integrative review. We have found studies that evaluated the cytotoxic activity of 21 species of Erythroxylum against 45 different cell lines, the analysis of the chemical composition of these species shows that the metabolites present in each species influence the cytotoxic potential of them, especially the presence of disubstituted tropane alkaloids species with the highest cytotoxic potential. MTT and Sulforrodamine B assays were the main in vitro tests used for the evaluation of the cytotoxic activities. From the total of species, less than 10% of the Erythroxylum species have already been evaluated for the cytotoxic activity, four of them showed high cytotoxic activity according to with the criteria of the NCI plant screening program. Thus, this genus represents a potential source of natural products with antitumor activity.

Diallyl Disulfide Attenuates Methotrexate-Induced Hepatic Oxidative Injury, Inflammation and Apoptosis and Enhances its Anti-tumor Activity

2021 ◽  
Vol 14 ◽  
Author(s):  
Marwa M. Khalaf ◽  
Emad H.M. Hassanein ◽  
Abdel-Gawad S. Shalkami ◽  
Ramadan A.M. Hemeida ◽  
Wafaa R. Mohamed
Keyword(s):  
Cytotoxic Activity ◽  
Caspase 3 ◽  
In Vitro Study ◽  
Diallyl Disulfide ◽  
Sod Activity ◽  
Cytotoxic Effects ◽  
Wide Range ◽  
Anti Tumor Activity ◽  
Mcf 7

Background: Methotrexate (MTX) is used potently for a wide range of diseases. However, hepatic intoxication by MTX hinders its clinical use. Objectives: The present study was conducted to investigate the diallyl disulfide (DADS) ability to ameliorate MTX-induced hepatotoxicity. Methods: Thirty-two rats were randomly divided into four groups: normal control, DADS (50 mg/kg/day, orally), MTX (single i.p. injection of 20 mg/kg) and DADS+MTX. Liver function biomarkers, histopathological examinations, oxidative stress, inflammation, and apoptosis biomarkers were investigated. Besides, an in vitro cytotoxic activity study was conducted to explore the modulatory effects of DADS on MTX cytotoxic activity using Caco-2, MCF-7, and HepG2 cells. Results: DADS significantly reduced the increased serum activities of ALT, AST, ALP, and LDH. These results were confirmed by the alleviation of liver histopathological changes. It restored the decreased GSH content and SOD activity, while significantly decreased MTX-induced elevations in both MDA and NO2- contents. The hepatoprotective effects were mechanistically mediated through the up-regulation of hepatic Nrf-2 and the down-regulation of Keap-1, P38MAPK, and NF-κB expression levels. In addition, an increase in Bcl-2 level with a decrease in the expression of both Bax and caspase-3 was observed. The in vitro study showed that DADS increased MTX anti-tumor efficacy. Conclusions: DADS potently alleviated MTX-induced hepatotoxicity through the modulation of Keap-1/Nrf-2, P38MAPK/NF-κB and apoptosis signaling pathways and effectively enhanced the MTX cytotoxic effects, which could be promising for further clinical trials.

scholarly journals IN-VITRO HYPOGLYCEMIC EVALUATION OF FRACTIONS OF HYDRO-ACOHOLIC EXTRACT OF HEARTWOOD OF TECOMELLA UNDULATA LINN.

2017 ◽  
Vol 10 (2) ◽  
pp. 266
Author(s):  
Munish Garg ◽  
Ruby Rohilla ◽  
Chanchal Garg
Keyword(s):  
Postprandial Hyperglycemia ◽  
Alcoholic Extract ◽  
Phytochemical Screening ◽  
Bioactive Components ◽  
Standard Drug ◽  
Minimal Inhibitory Concentrations ◽  
Glucosidase Inhibitors ◽  
Ic50 Values ◽  
Tecomella Undulata

Objective: To screen α-amylase and α-glucosidase inhibitors from the different fractions of crude hydro-alcoholic extract of heartwood of Tecomella undulata Linn.Methods: Four fractions of crude hydro-alcoholic extract of heartwood of plant were used for in-vitro inhibitory assays against digestive enzymes: α-amylase and α-glucosidase. For assay, different concentrations (20, 40, 60, 80, 100 µg/ml) were used for all fractions. Standard protocol was used for preliminary phytochemical screening of different bioactive components present in all fractions.Results: The fractions have shown moderate to highest inhibitory activity against both enzymes. But, the strong inhibition was revealed by acetone fraction against α-amylase with very minimal inhibitory concentrations at IC50 values when compared with a standard drug acarbose. Several medicinally active phytocomponents such as flavanoids, saponin, anthraquinones, tannins, triterpenoids and phenols were observed in all studied fractions.Conclusion: The different fractions prepared from crude hydro-alcoholic extract of heartwood of plant are capable of inhibiting α-amylase and α-glucosidase and it can be concluded that heartwood of Tecomella undulata Linn. is partially active against postprandial hyperglycemia, thus diabetes mellitus.Keywords: Tecomella undulata Linn., Diabetes mellitus, α-Amylase, α-Glucosidase. 

scholarly journals The activity of alcoholic extract of Garlic on the growth of Staphylococcus aureus with estimation of median lethal dose in lab. Mice

2010 ◽  
Vol 9 (2) ◽  
pp. 53
Author(s):  
E. M. Rashid, Sh. Jamal, and Sh. M. Al-khazraji
Keyword(s):  
Lethal Dose ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Alcoholic Extract ◽  
Staph Aureus ◽  
Median Lethal Dose ◽  
Highly Active ◽  
Inhibition Of Growth ◽  
Ethanolic Extraction

The study was intended to investigate the in vitro activity of alcoholic extract of Garlic on the inhibition of growth of Staph. aureus which was isolated from skin infections , and determine the median lethal dose ( LD 50 ) of the extract in lab. mice. The Garlic was extracted by ethyl alcohol 95% , the ratio of ethanolic extraction amounted 44% of the weight of dry substance. Graduated concentration were prepared from alcoholic extract of Garlic from 10-100 mg/ml. Their activities were checked up against Staph.aureus by agar diffusion method using ethylene glycol as control. The results showed that the sensitivity of the test bacteria was gradually increased with increasing the extract concentration , the concentration 10-30 mg/ml were rather low active in preventing the growing of Staph. aureus in culturing media , the concentrations 40-70 mg/ml were moderately active ,meanwhile the concentrations 80-100 mg/ml were highly active against the growing of Staph.aureus . The results also showed that the LD50 of the ethanolic extract of Garlic when it is orally administered to the lab mice by gradual concentrations was about 8000 mg/kg body weight . The toxic signs during 24 hrs after initial feeding with the extract were rapid breathing followed by dullness , then death

scholarly journals Evaluation of the cytotoxic potential of extracts from the genus Passiflora cultived in Brazil against cancer cells

2018 ◽  
Author(s):  
Ricardo Guimarães Amaral ◽  
Silvana Vieira Floresta Gomes ◽  
Ângelo Roberto Antoniolli ◽  
Maria Claudia dos Santos Luciano ◽  
Cláudia do Ó Pessoa ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Death ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Cytotoxic Potential ◽  
Leaf Extracts ◽  
High Cytotoxic Activity ◽  
Apoptosis And Necrosis

AbstractThis work aimed to evaluate the cytotoxic potential against cancer cells of Passiflora genus plant species cultivated in Brazil and identify the mechanism of cytotoxicity induced by the most promising extract. Leaf extracts from 14 Passiflora (P.) species were obtained ASE and in vitro cytotoxicity evaluated against cancer cell lines using MTT assay at a single concentration of 50 μg/mL. Additionally, the IC50 of the P. alata (ELPA) leaf extracts was determined against both tumor (HCT-116, SF-295, OVACAR-8, and HL-60), and non-tumor cells (PBMC). The ELPA flavonoids were identified by HPLC-DAD and UHPLC-MS/MS. The morphological analyses used light and fluorescence microscopy, and cell cycle and DNA fragmentation analyses used flow cytometry to determine the mechanism of cell death induced by ELPA in HL-60. Among the Passiflora leaf extracts evaluated; ELPA stood out with high cytotoxic activity, followed by P. capsularis and P. quadrangulares with varying high and low cytotoxic activity. ELPA presented high cytotoxic potency in HL-60 (IC50 19.37 μg/mL), yet without cytotoxic activity against PBMC, suggesting selectivity for tumor cells. The cytotoxic activity of ELPA may well be linked to the presence of ten identified flavonoids. Cells treated with ELPA presented the hallmarks typical of apoptosis and necrosis, with cell cycle arrest in the G2/M phase. Conclusion: From among the studied species, ELPA presented greater cytotoxic activity, possibly a consequence of synergistic flavonoid action which induces cell death by apoptosis and necrosis.

scholarly journals Ethanolic extract of ocimum kilimandscharicum linnleaves: phytochemical and in vitro pharmacological activity

2021 ◽  
pp. 106-109
Author(s):  
Yamini N ◽  
Lahari S ◽  
Phani deepthi V
Keyword(s):  
In Vitro Model ◽  
Ethanolic Extract ◽  
Clinical Development ◽  
Dependent Manner ◽  
Thrombolytic Activity ◽  
Standard Drug ◽  
Ethanolic Extracts ◽  
Vitro Model ◽  
Dose Dependent

Using an in vitro model, the anti-thrombolytic efficacy of ethanolic extracts of Ocimum kilimandscharicum Linn was investigated. The researchers discovered that different concentrations of the extract had significant anti-thrombolytic activity in a dose-dependent manner , which was comparable to a standard drug. As a result of the presence of flavonoids and polyphenols in the plant extract, it can be concluded that it has a promising future in the treatment of thrombosis. This knowledge will be useful in the clinical development of thrombolytic therapeutics by identifying more potent anti-thrombolytic principles from natural resources..    

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