scholarly journals AKTIVITAS ANTIBAKTERI ISOLAT KAPANG ENDOFIT DARI KULIT NANAS (Ananas comosus (L.) Meer)

2021 ◽  
Vol 4 (02) ◽  
pp. 20-30
Author(s):  
Siti Hamidatul Aliyah ◽  
Musfirotun Musfirotun ◽  
Nur Antriana
Keyword(s):  
Staphylococcus Aureus ◽  
Inhibition Zone ◽  
Ananas Comosus ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Pineapple Peel ◽  
Living Organisms

Pineapple contains an enzyme called bromelain which is can be used as antiseptic of mouth, antibacterial, antifungal, and disinfectant. Endophytic mold is a microbe that forms colonies in healthy tissues of living organisms, generally, endophytic microbes do not cause harmful symptoms in the tissue of their host. This study aims to isolate the endophytic shell origin of pineapple peel that has acted as an antibacterial. A total of 3 endophytic capsules, Ac-I, Ac-II and Ac-III were isolated from pineapple skin using PDA media. The three isolates were purified and microscopic examinations were performed. Antibacterial testing was performed by fermentation to produce supernatant, then tested using disc method (Kirby-Bauer method) with Staphylococcus aureus and Escerichia coli test bacteria. The 3 isolates obtained only 1 isolate Ac-III isolates that have activity as antibacterial, with the inhibition zone diameter in bacterium Staphylococcus aureus 7.65 mm while in the bacterium Escerichia coli 6,9 mm.

Karakteristik Fisik dan Aktivitas Antibakteri Sabun Cair Minyak Nilam (Pogostemon cablin Benth.) yang Berbasis Surfaktan Sodium Lauril Eter Sulfat

2020 ◽  
pp. 1-10
Author(s):  
Yuni Anggraeni ◽  
Fakhrun Nisa' ◽  
Ofa Suzanti Betha
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Foam Height ◽  
Inhibition Zone ◽  
Positive Control ◽  
Centrifuge Test ◽  
Liquid Soap ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Pogostemon Cablin

Patchouli oil obtained from patchouli plants (Pogostemon cablin Benth.) has been widely used as an antibacterial agent in liquid and solid soap preparation. In this research, patchouli oil was added to liquid soap formula which use sodium lauryl ether sulphate (SLES) surfactant. The purpose of this study was to determine the physical characteristics and antibacterial activity of patchouli oil liquid soaps based on SLES surfactant. The Liquid soaps were prepared into four formulas with variation of patchouli oil concentration that were F0 (0.00% w/w), F1 (0.05% w/w), F2 (0.50% w/w), and F3 (1.00% w/w). The liquid soaps were evaluated including organoleptic, microscopic property, physical stability by using centrifuge test, pH, foam height and stability, viscosity and rheology, and antibacterial activity against Staphylococcus aureus ATCC 25923 by using disc diffusion method and chloramphenicol as a positive control. The results showed that all liquid soap formulas had following characteristics: translucent except F3 slightly murky, homogen, stable after centrifuge test, pH between 6.55 – 6.66 that were no significantly different and met SNI standards of liquid soap, foam height between 2.67 – 3.23 cm that were no significantly different and stable after 5 minutes of testing, viscosity between 2237 – 2397 cPs relatively equal in all formulas with pseudoplastic rheological properties. The patchouli oil liquid soaps had antibacterial activity against Staphylococcus aureus ATCC 25923, where F0, F1, F2, F3 and positive control result in inhibition zone diameter of 11.26±2.60 mm, 14.60 ± 2.45mm, 15.51 ± 0.44 mm, 17.97 ± 0.71 mm and 19.00 ± 3.92 mm respectively. Based on the ANOVA one way test, the addition of 1% patchouli oil gave a significant effect on the inhibition zone diameter compared to F0 (without patchouli oil)

scholarly journals Efek Antibakteri Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.) dan Sediaan Masker Gel ¬Peel-Off Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.)

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 112
Author(s):  
Theresia H. Tunas ◽  
Hosea Jaya Edy ◽  
Jainer Pasca Siampa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Moringa Oleifera ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Moringa Oleifera Leaves ◽  
Inhibitory Power ◽  
Moringa Oleifera Lam

Daun Kelor (Moringa oleifera Lam.) bermanfaat untuk menghambat aktivitas pertumbuhan bakteri karena adanya kandungan senyawa flavonoid , alkaloid, dan fenol. Penelitian ini bertujuan untuk menguji aktivitas antibakteri dari ekstrak etanol daun Kelor dan sediaan masker gel peel-off ekstrak etanol daun Kelor terhadap bakteri Staphylococcus aureus. Penelitian ini menggunakan metode eksperimental laboratorium, ekstrak etanol daun kelor dan sediaan masker gel peel-off ekstrak etanol daun Kelor dibuat dengan konsentrasi 1, 3, 5, 7, dan 9%. Ekstrak daun Kelor diperoleh dengan cara maserasi dengan menggunakan pelarut etanol 96%. Pada pengujian antibakteri menggunakan metode sumuran. Diameter zona hambat paling besar yang ditimbulkan oleh ekstrak etanol daun Kelor yaitu pada konsentrasi 7% dengan diameter zona hambat sebesar 5,75 mm, sedangkan pada sediaan masker gel peel-off tidak menunjukkan adanya daya hambat. Dapat disimpulkan bahwa ekstrak etanol daun Kelor memiliki aktivitas antibakteri yang termasuk dalam kategori sedang.Moringa oleifera Lam. (Moringa oleifera) leaves are useful for inhibiting bacterial growth activity due to the presence of flavonoid, alkaloid, and phenol compounds. This study aims to test the antibacterial activity of the Moringa leaf ethanol extract and the gel peel-off mask preparation of Moringa leaf ethanol extract against Staphylococcus aureus bacteria. This study uses laboratory experimental methods, Moringa leaf ethanol extract and gel mask peel-off preparation for Moringa leaf ethanol extract made with concentrations of 1, 3, 5, 7, and 9%. Moringa leaf extract was obtained by maceration using 96% ethanol solvent. In antibacterial testing using the well method. The biggest inhibition zone diameter caused by ethanol extract of Moringa leaves is at a concentration of 7% with a inhibition zone diameter of 5.75 mm, whereas the peel-off gel mask preparation did not show any inhibitory power. It can be concluded that the ethanol extract of Moringa leaves has antibacterial activity which is included in the medium category.

scholarly journals DAYA HAMBAT EKSTRAK ETANOL Schismatoglottis sp. TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coli

2015 ◽  
Vol 17 (1) ◽  
pp. 149
Author(s):  
Fahri Bian ◽  
Febby E.F. Kandou ◽  
Marhaenus J. Rumondor
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter ◽  
Paper Disk

DAYA HAMBAT EKSTRAK ETANOL Schismatoglottis sp. TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coliABSTRAK Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak etanol daun Schismatoglottis sp. terhadap bakteri Staphylococcus aureus dan Escherichia coli. Daya hambat ekstrak etanol Schismatoglottis sp. terhadap bakteri menggunakan metode Kirby-Bauer, yaitu metode difusi dengan cakram kertas. Ekstrak etanol Schismatoglottis sp. menghambat pertumbuhan S. aureus pada konsentrasi 60% dan 90% dengan diameter zona hambat 16,10 mm dan 31,33 mm serta  terhadap E. coli pada konsentrasi 30%, 60%, dan 90% dengan diameter zona hambat berturut-turut 11,72 mm, 16,38 mm, dan  29,27 mm. Berdasarkan hasil penelitian, dapat disimpulkan bahwa ekstrak etanol Schismatoglottis sp. terhadap S. aureus pada konsentrasi 60% dan 90% masing-masing memiliki kekautan antibakteri termasuk kategori kuat dan sangat kuat, sedangkan terhadap E. coli pada konsentrasi 30% dan 60%, memiliki kekuatan antibakteri kategoti kuat sedangkan pada konsentrasi 90% memiliki kekuatan antibakteri kategori sangat kuat. Kata kunci : Schismatoglottis sp, Antibakteri, Staphylococcus aureus, Escherichia coli THE INHIBITION OF ETHANOL EXTRACT OF Schismatoglottis sp. AGAINST BACTERIA Staphylococcus aureus AND Escherichia coli. ABSTRACT This study aimed to determine the inhibition of ethanol extract of leaves Schismatoglottis sp. against S. aureus and E. coli. Inhibition of the ethanol extract of Schismatoglottis sp. against to bacterial using the Kirby-Bauer method, is the paper disk diffusion method. Schismatoglottis sp. ethanol extract inhibit the growth of S. aureus at a concentration of 60% and 90% inhibition zone with a diameter of 16.10 mm and 31.33 mm and against E. coli at a concentration of 30%, 60%, and 90% with inhibition zone diameter respectively 11, 72 mm, 16.38 mm and 29.27 mm. Based on the results, it can be concluded that the ethanol extract Schismatoglottis sp. against S. aureus at concentrations of 60% and 90% respectively have antibacterial kekautan categorized as strong and very strong, while against E. coli at a concentration of 30% and 60%, have a strong kategoti antibacterial strength while at a concentration of 90% has antibacterial strength very strong category. Keywords: Schismatoglottis sp, antibacterials, Staphylococcus aureus, Escherichia coli.

scholarly journals PERBANDINGAN AKTIVITAS ANTIBAKTERI EKSTRAK KASAR RESIN DAN DAUN GAHARU (Gyrinops versteegii)

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
I Gde Adi Suryawan Wangiyana ◽  
. Akram ◽  
Farouq Isbulloh
Keyword(s):  
Staphylococcus Aureus ◽  
Standard Error ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Extraction Solvent ◽  
Extract Concentration ◽  
Antibacterial Assay ◽  
Resin Extract

Comparison of Antibacterial Activities of Agarwood (Gyrinops versteegii) Resin and Leaf Crude ExtractThis research aim is to compare antibacterial activities of leaves extract and resin extract of Gyrinops versteegii with different extraction solvent and concentration. Leaves and resin had been prepared by drying and grinding then were extracted by maceration method. Factorial experiment design was used for extract application to Staphylococcus aureus ATCC25923. First factor was extract ingredient, second factor was extraction solvent, third factor was extract concentration. Inhibition zone as main parameter for antibacterial assay were analysed by ANOVA, HSD test and standard error. The inhibition zone of resin was higher than inhibition zone of leaves. The extraction solvent and extract concentration were not significantly resulted in different inhibition zone diameter. However, there were unique interaction between extraction solvent and extraction concentration that affected inhibition zone diameter. It could be concluded that the inhibition zone of resin was higher than that of leaves while no significant result from extraction solvent and extract concentration factorsKeywords: antibacterial, agarwood, extract, leaves, resin ABSTRAKPenelitian ini bertujuan untuk membandingkan aktivitas antibakteri daun gaharu dan resin gaharu G. versteegii menggunakan beberapa pelarut pengekstrak dan konsentrasi ekstrak yang berbeda. Bahan daun dan resin gaharu dikeringkan dan dicacah, kemudian diekstrak menggunakan metode maserasi. Uji aplikasi ekstrak pada Staphylococcus aureus ATCC25923 dilakukan menggunakan rancangan faktorial, yaitu faktor pertama bahan ekstrak (resin, daun), faktor kedua pelarut pengekstrak (etanol, metanol), dan faktor ketiga konsentrasi ekstrak (0,25; 0,5; dan 1 g mL–1). Data zona hambat terhadap bakteri uji dianalisis menggunakan ANOVA, BNJ dan standard error. Rerata zona hambat ekstrak daun gaharu (7 mm) lebih besar secara signifikan dibandingkan dengan rerata zona hambat ekstrak resin (6,5 mm). Faktor pelarut pengekstrak dan konsentrasi ekstrak tidak berpengaruh signifikan terhadap zona hambat. Pelarut pengekstrak dan konsentrasi ekstrak memberikan pengaruh interaksi berbeda-beda terhadap zona hambat. Dapat disimpulkan bahwa bahan ekstrak daun gaharu memiliki aktivitas antibakteri lebih bagus dibandingkan dengan ekstrak resin gaharu dengan konsentrasi ekstrak efisien sebesar 0,25 g mL–1.

scholarly journals UJI DAYA HAMBAT EKSTRAK METANOL Selaginella delicatula DAN Diplazium dilatatum TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

2015 ◽  
Vol 15 (1) ◽  
pp. 52 ◽  
Author(s):  
Stery B. Oroh ◽  
Febby E.F. Kandou ◽  
Johanis Pelealu ◽  
Dingse Pandiangan
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Methanol Extract ◽  
Base Material ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter

ABSTRAK Penelitian ini bertujuan untuk menguji daya hambat ekstrak metanol Selaginella delicatula dan Diplazium dilatatum terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Pengujian daya hambat ekstrak terhadap bakteri menggunakan metode Kirby-Bauer, yaitu metode difusi dengan cakram kertas. Ekstrak S. delicatula menghambat pertumbuhan S. aureus pada konsentrasi ekstrak 30%, 60% dan 90% dengan diameter zona hambat berturut-turut 0.00 mm, 6.70 mm dan 7.80 mm; serta menghambat pertumbuhan E. coli dengan diameter zona hambat berturut-turut 0.00 mm, 8.40 mm dan 13.00 mm. Ekstrak D. dilatatum menghambat pertumbuhan S. aureus pada konsentrasi ekstrak 30%, 60% dan 90% dengan diameter zona hambat berturut-turut 0.00 mm, 0.00 mm dan 6.70 mm; serta menghambat pertumbuhan E. coli dengan diameter zona hambat berturut-turut 0.00 mm, 0.00 mm dan 8.00 mm. Berdasarkan hasil penelitian yang diperoleh, dapat disimpulkan bahwa ekstrak S. delicatula dan D. dilatatum tergolong dalam kategori kuat dan sedang serta memiliki potensi sebagai bahan dasar antibakteri, karena kedua ekstrak menunjukkan daya hambatnya. Kata kunci : tumbuhan paku, Selaginella delicatula, Diplazium dilatatum, Antibakteri, Staphylococcus aureus, Escherichia coli INHIBITION TEST OF METHANOL EXTRACT OF Selaginella delicatula AND Diplazium dilatatum AGAINST BACTERIA Staphylococcus aureus AND Escherichiacoli ABSTRACT This study aimed to test the inhibition of methanol extract of Selaginella delicatula and Diplazium dilatatum on the growth of Staphylococcus aureus and Escherichia coli. Testing inhibition of the extracts to bacterial using the Kirby-Bauer method, is the paper disk diffusion method. S. delicatula extract inhibited the growth of S. aureus at concentrations of extract 30%, 60% and 90% inhibition zone diameter respectively 0.00 mm, 6.70 mm and 7.80 mm; and inhibit the growth of E. coli with inhibition zone diameter respectively 0.00 mm, 8.40 mm and 13.00 mm. D. dilatatum extract inhibited the growth of S. aureus at concentrations of extract 30%, 60% and 90% inhibition zone diameter respectively 0.00 mm, 0.00 mm and 6.70 mm; and inhibit the growth of E. coli with inhibition zone diameter respectively 0.00 mm, 0.00 mm and 8.00 mm. Based on the results obtained, it can be concluded that the extract of S. delicatula and D. dilatatum classified in the category of medium and strong and has potential as a base material for both extracts showed antibacterial inhibitory power. Keywords: ferns, Selaginella delicatula, Diplazium dilatatum, Antibacterial, Staphylococcus aureus, Escherichia coli.

scholarly journals Uji antibakteri kombinasi ekstrak daun Biduri (Calotropis gigantea L.) dan daun Bandotan (ageratum conyzoides L.)

2020 ◽  
Vol 1 (2) ◽  
pp. 165
Author(s):  
Munira Munira ◽  
Fina Rodisa ◽  
Muhammad Nasir
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Ageratum Conyzoides ◽  
Inhibition Zone Diameter ◽  
Calotropis Gigantea ◽  
Microbiological Test ◽  
Zone Diameter

Background: Biduri leaves (Calotropis gigantea (L.) W.T. Aiton and Bandotan leaves (Ageratum conyzoides L.) are wild growing plants that are believed to be used as an antibacterial.Objectives: This research was conducted to determine the effect of combination ethanolic extract of Biduri and Bandotan leaves against Staphylococcus aureus and Staphylococcus epidermidis.Methods: This research is an experimental by using Completely Randomized Design (CRD) with 4 treatments which is P0 (aquadest), P1 (Biduri leaf extract), P2 (Bandotan leaf extract), and P3 (combination of Biduri and Bandotan leaf extract) with 5 replications. Microbiological test used was the diffusion method.Results: The results of Anova showed that combination of Biduri and Bandotan leaf extract very influential in the growth of Staphylococcus aureus dan Staphylococcus epidermidis. Duncan further test results showed that the largest average inhibition zone diameter in inhibiting Staphylococcus aureus was formed by P2 which is 14.14 mm, significantly different from P1 (9.46 mm) and P3 (11.20 mm), but P1 is not significantly different from P3. Toward Staphylococcus epidermidis, the largest average inhibition zone diameter also formed by P2 which is 17.06 mm that is also significantly different from P1 (10.14 mm) and P3 (12.66 mm), and P1 is not significantly different from P3.Conclusion: The combination of Biduri and Bandotan leaf extract very influential against Staphylococcus aureus and Staphylococcus epidermidis.

scholarly journals Pengaruh lama maserasi daun ketapang merah (Terminalia Catappa L.) terhadap daya hambat Staphylococcus aureus dan Escherichia coli

2021 ◽  
Vol 2 (2) ◽  
pp. 158
Author(s):  
Berwi Fazri Pamudi ◽  
Munira Munira ◽  
Rizky Amalia Saha ◽  
Muhammad Nasir
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Terminalia Catappa ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Background: Bengal Almond leaves (Terminalia catappa L.) contain alkaloids, saponins, tannins, polyphenols, quinones, flavonoids and triterpenoids that can function as antibacterial.Objectives: This research was conducted to determine the effect of maceration time of red ketapang leaves (Terminalia catappa L.) on the growth of Staphylococcus aureus and Escherichia coli. Methods: This study was experimental using a completely randomized design (CRD) consisting of 6 treatments, that P0 (aquades), P1 (red ketapang leaf extract with 1 day maceration time), P2 (2 days maceration) P3 (3 days maceration) , P4 (4 days maceration) and P5 (5 days maceration) with 4 replications.Results: Anova test showed that macerated red ketapang leaf extract significantly affected the growth of Staphylococcus aureus and Escherichia coli (p= 0.000). Duncan's further test showed that the largest inhibition zone diameter in Staphylococcus aureus was formed by P2 (2 days maceration time) which was 18.62 mm and there was no significant difference between treatments. Meanwhile, the largest inhibition zone diameter against Escherichia coli was formed by P1 (1 day maceration time) which was 20.25 mm and there was a significant difference between treatments. Conclusion: Ketapang red leaf extract with different maceration times can inhibit the growth of Staphylococcus aureus and Escherichia coli.

scholarly journals Uji Efek Antibakteri Chromodoris annae terhadap Bakteri Staphylococcus aureus dan Escherichia coli

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Aqueline N. Faidiban ◽  
Jimmy Posangi ◽  
Pemsi M. Wowor ◽  
Robert A. Bara
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
The Mean ◽  
Escherichia Coli Bacteria

Abstract: This study was aimed to evaluate the antibacterial effect of Chromodoris annae taken from Bunaken waters. This was an experimental study. The antibacterial effect was tested by using the Kirby-Bauer method. Chromodoris annae extract was made by maceration using 95% ethanol and was tested to Staphylococcus aureus and Escherichia coli bacteria. Ciprofloxacin was used as the positive control and aquadest as the negative control. The results showed that the mean inhibition zone diameter of Chromodoris annae extract to Staphylococcus aureus was 22.3 mm meanwhile of ciprofloxacin was 34.7 mm. The mean inhibition zone diameter of Chromodoris annae extract to Escherichia coli was 23.0 mm meanwhile of ciprofloxacin was 40.3 mm. Moreover, aquadest showed no inhibition zone. In conclusion, Chromodoris annae had very strong antibacterial effect to the growths of Staphylococcus aureus and Escherichia coli.Keywords: Chromodoris annae, Staphlococcus aureus, Escherichia coli Abstrak: Penelitian ini bertujuan untuk mengevaluasi efek antibakteri dari Chromodoris annae yang diambil dari perairan Bunaken. Jenis penelitian ialah eksperimental. Pengujian aktivitas antibakteri menggunakan metode Kirby-Bauer. Ekstrak Chromodoris annae dibuat dengan cara maserasi menggunakan pelarut etanol 95% dan diujikan aktivitas antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli. Sebagai kontrol positif digunakan antibiotik ciprofloxacin dan sebagai kontrol negatif digunakan akuades. Hasil penelitian memperlihatkan rerata zona hambat ekstrak Chromodoris annae terhadap Staphylococcus aureus sebesar 22,3 mm sedangkan zona hambat ciprofloxacin sebesar 34,7 mm. Rerata zona hambat ekstrak Chromodoris annae terhadap Escherichia coli sebesar 23,0 mm sedangkan zona hambat ciprofloxacin sebesar 40,3 mm. Akuades tidak memperlihatkan adanya zona hambat. Simpulan penelitian ini ialah Chromodoris annae memiliki efek antibakteri kategori sangat kuat terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli.Kata kunci: Chromodoris annae, Staphylococcus aureus, Escherichia coli

scholarly journals Antifungal and growths regulation activity of new metal-organic compounds

2019 ◽  
Vol 16 (2) ◽  
pp. 143-149
Author(s):  
O. V. Bashta ◽  
L. P. Pasichnyk ◽  
N. M. Voloshchuk ◽  
G. G. Repich ◽  
O. O. Zholob ◽  
...  
Keyword(s):  
Seed Germination ◽  
Organic Compounds ◽  
Copper Complex ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Chelate Complexes ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Grain Crops ◽  
Metal Organic

The aim of present paper is to study the antifungal activity of the new platinum, palladium and copper chelate complexes with different organic ligands against Fusarium strains and to determine the influence of these compounds on the germination energy and seed germination of investigated grain crops. Methods. In vitro antifungal activities of new chelate complexes were studied by the agar disk diffusion method. New complexes were tested against pathogenic Fusarium strains such as — F. culmorum 3260/4, F. verticillioides and F. sporotrichioides. The toxic effect of new complexes was evaluated according to changes in germination power and seed germination of grain crops — winter wheat, vernal barley and maize. The determination of these characteristics was carried out in accordance with the standard demands. Results. Our results revealed that palladium complex H exhibited fungicidal activity against F. culmorum 3260/4 and fungistatic activity against F. verticillioides with growth inhibition zone diameter 25 mm ad 20 mm respectively. Copper complex 2 showed a moderate fungicidal effect against F. culmorum 3260/4 strain (inhibition zone diameter 12 mm). The treatment of grain crops seeds with the H and 2 complexes demonstrated the less toxicity than standard agent — commercial fungicide tebuconazol. These complexes had also less negative influence on the germination power for all test cultures than tebuconazol. Copper complex 2 showed a noticeable growth promoting effect on studied seeds compared to seeds under conditions without treatment. Conclusions. Thus, the studied complexes can be considered as promising antifungal agents with growth regulating properties. The directional modification of the complexes to obtain the more potent derivatives will be performed.Keywords: metal-organic compounds, antifungal, growths regulation activity.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BAWANG DAYAK (Eleutherine polmifolia (L.) Merr) TERHADAP Salmonella typhi dan Escherichia coli

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Nofita Nofita ◽  
Keyword(s):  
Escherichia Coli ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Skin Infections ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Agar Diffusion ◽  
Escherichia Coli Bacteria

Dayak onion (Eleutherine polmifolia (L.) Merr) was a typical plant of Kalimantan. Empirically dayak onion (Eleutherine polmifolia (L.) Merr) used by the comunity to treat skin infections. The purpose of this study was to determine the antibacterial activity of ethanol extract of dayak onion power against Salmonella typhi and Escherichia coli bacteria. The highest concentration used was 100%, and the lowest concentration was 0,5%. As a control of cloramfenikol for Salmonella typhi and ciprofloxacin for Escherichia coli bacteria. This research method used agar diffusion. The results showed the ethanol extract of dayak onion (Eleutherine polmifolia (L.) Merr) contained flavonoids, alkaloids, tannins and phenols with the highest levels was by tannins at 7,45%. Dayak onion (Eleutherine polmifolia (L.) Merr) are inhibiting the growth of Salmonella typhi bacteria at a minimum inhibitory concentration (MIC)of 1% with a inhibition zone diameter of 6,24 mm and 0,9% with a inhibition zone diameter of 6,20 mm at Escherichia coli bacteria.Keywords : Eleutherine polmifolia (L.) Merr, Salmonella typhi, Escherichia coli, Agar diffusion, MIC

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