MORPHOFUNCTIONAL CHARACTERISTICS OF LEAVES AND FRUITS IN MALOIDEAE (ROSACEAE): b. THE ROLE OF SURFACE TISSUES IN THE FORMATION OF RESISTANCE TO FUNGAL DISEASES

Background. Resistance to the effects of plant biotic stressors is determined by a set of factors. Among them, the leading protective role is often assigned to the physiological and biochemical characteristics of the surface tissues. However, one cannot ignore the specificity of the microstructural organization of the plant surface, since the nature of interactions in phytopathogenic organisms is more complex than the chemical impact. Meanwhile, the information accumulated to date about the structure of the surface tissues of the vegetative and reproductive organs of plants, and the interface of mycobiota, is fragmentary.Objective. Mature leaves and fruits taken from representatives of the subfamily Maloideae Werber (Malus domestica Borkh., Pyrus communis L., Cydonia oblonga Mill. and Mespilus germanica L.) were selected for the study.Materials and methods. Samples for the research were taken from the middle part of the crown of model trees in 3 replications. In recent years, scanning electron microscopy (SEM) with cryofixation is considered the most promising technique and is used to analyze the surface of biological organisms and identification of species. It is particularly informative in the case of organisms with complex surface micromorphology and for studying the biodiversity of pathogens. However, in this work we combined the methods of light, electron (SEM, TEM) and confocal microscopy. The samples were also studied using phytopathological and histochemical techniques. Condensed polyphenols were detected using K2Cr2O7 and FeCl3 as well as 4-(Dimethylamino)cinnamaldehyde (DMACA, Sigma-Aldrich).Results. On the basis of the obtained phytopathological materials and published data an overview of fungal diseases afflicting leaves and fruits of M. domestica, P. communis, C. oblonga and M. germanica was made. It has been established that a common feature of the Maloideae fruits is the accumulation of condensed polyphenols, which play an important protective role against biotic stressors, in the cells of the pericarp’s outer tissues. Anatomical and morphological characteristics of passive immunity, or horizontal resistance to fungal pathogens, include the specific nature of waxy and cuticular deposits, features of the formation of cuticular folds and peristomatic rings in the stomata area and microstrands at the base of trichomes, thickness of the cuticle and cork tissue, and the development of lenticels on fruits.Conclusion. The studied model plants suffer from a sufficiently wide range of diseases with different etiologies; among them, the most widespread and harmful are mycoses. In view of this, their resistance to fungal pathogens correlates with the specificity of the leaf and fruit surface microstructure and the content of phenolic substances (polyphenols) in the cells of the pericarp’s surface tissues.

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NEW RED GRAPE WINE VARIETY OF KRASNOSTOP CARPI

Izvestiâ Timirâzevskoj selʹskohozâjstvennoj akademii ◽

10.26897/0021-342x-2020-5-40-48 ◽

2020 ◽

pp. 40-48

Author(s):

N.A. DURAN ◽

Keyword(s):

Selection Process ◽

Sugar Content ◽

Morphological Characteristics ◽

Fungal Diseases ◽

Wide Range ◽

Modern Economic ◽

Biological Plasticity ◽

Pesticide Load ◽

Red Grape ◽

Grape Varieties

The selection process for getting the perfect variety is continuous and always relevant. In the context of the development of domestic viticulture and winemaking, modern economic and environmental conditions require a wide range of grapes that have a set of various economically valuable qualities, including high adaptability, biological plasticity, combined with consistently high quality of the crop. It is especially important to develop competitive, highly profitable grape varieties that do not require a large pesticide load and are resistant to frost and fungal diseases.The paper presents the results of agrobiological study of the red Krasnostop Carpi wine grape variety. Agrobiological and morphological characteristics of the variety are given according to research data for 3 years. The authors carried out ampelometric screening of the leaf to certify the variety.The Krasnostop Carpi is a mid-late ripeness variety of grape. The weight of berry is 1.9–2.0 g.The skin is of medium thickness. The pulp is meaty and juicy. The taste is harmonious. Sugar content is 220–240 g/dm3, acidity – 6.3 g/dm3. The variety can withstand frost down to – 25–27°С, resistant to mildew and powdery mildew. The Krasnostop Carpi variety is promising for cultivation in the northern industrial viticulture of Russia. The variety passed State testing in 2018, it features high biological plasticity: resistance to thermal stress and fungal diseases. It is used for the production of dry wines of high quality.The authors recommend the variety for commercial growing in Region 6 using the uncovered growing technology with two preventive treatments against fungal diseases.

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It is better an approximate answer to the right question than the exact answer to the wrong question : the case of the psychometric analysis of the ASQ:SE

10.31234/osf.io/a5tdf ◽

2020 ◽

Author(s):

Luis Anunciacao ◽

janet squires ◽

J. Landeira-Fernandez

Keyword(s):

Internal Structure ◽

Statistical Methods ◽

Principal Component ◽

Psychological Theory ◽

Published Data ◽

Multivariate Statistical ◽

Exact Answer ◽

Wide Range ◽

Ages And Stages Questionnaire ◽

The Right

One of the main activities in psychometrics is to analyze the internal structure of a test. Multivariate statistical methods, including Exploratory Factor analysis (EFA) and Principal Component Analysis (PCA) are frequently used to do this, but the growth of Network Analysis (NA) places this method as a promising candidate. The results obtained by these methods are of valuable interest, as they not only produce evidence to explore if the test is measuring its intended construct, but also to deal with the substantive theory that motivated the test development. However, these different statistical methods come up with different answers, providing the basis for different analytical and theoretical strategies when one needs to choose a solution. In this study, we took advantage of a large volume of published data (n = 22,331) obtained by the Ages and Stages Questionnaire Social-Emotional (ASQ:SE), and formed a subset of 500 children to present and discuss alternative psychometric solutions to its internal structure, and also to its subjacent theory. The analyses were based on a polychoric matrix, the number of factors to retain followed several well-known rules of thumb, and a wide range of exploratory methods was fitted to the data, including EFA, PCA, and NA. The statistical outcomes were divergent, varying from 1 to 6 domains, allowing a flexible interpretation of the results. We argue that the use of statistical methods in the absence of a well-grounded psychological theory has limited applications, despite its appeal. All data and codes are available at https://osf.io/z6gwv/.

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Control of Winged Waterprimrose (Jussiaea decurrens) and Northern Jointvetch (Aeschynomene virginica) with Fungal Pathogens

Weed Science ◽

10.1017/s0043174500044477 ◽

1979 ◽

Vol 27 (5) ◽

pp. 497-501 ◽

Cited By ~ 46

Author(s):

C. D. Boyette ◽

G. E. Templeton ◽

R. J. Smith

Keyword(s):

Oryza Sativa ◽

Glycine Max ◽

Fungal Pathogens ◽

Liquid Culture ◽

Field Experiments ◽

Colletotrichum Gloeosporioides ◽

Pathogenic Fungus ◽

Field Tests ◽

Wide Range ◽

Growing Region

An indigenous, host-specific, pathogenic fungus that parasitizes winged waterprimrose [Jussiaea decurrens(Walt.) DC.] is endemic in the rice growing region of Arkansas. The fungus was isolated and identified asColletotrichum gloeosporioides(Penz.) Sacc. f.sp. jussiaeae(CGJ). It is highly specific for parasitism of winged waterprimrose and not parasitic on creeping waterprimrose (J. repensL. var.glabrescensKtze.), rice (Oryza sativaL.), soybeans [Glycine max(L.) Merr.], cotton (Gossypium hirsutumL.), or 4 other crops and 13 other weeds. The fungus was physiologically distinct from C.gloeosporioides(Penz.) Sacc. f. sp.aeschynomene(CGA), an endemic anthracnose pathogen of northern jointvetch[Aeschynomene virginica(L.) B.S.P.], as indicated by cross inoculations of both weeds. Culture in the laboratory and inoculation of winged waterprimrose in greenhouse, growth chamber and field experiments indicated that the pathogen was stable, specific, and virulent in a wide range of environments. The pathogen yielded large quantities of spores in liquid culture. It is suitable for control of winged waterprimrose. Winged waterprimrose and northern jointvetch were controlled in greenhouse and field tests by application of spore mixtures of CGJ and CGA at concentrations of 1 to 2 million spores/ml of each fungus in 94 L/ha of water; the fungi did not damage rice or nontarget crops.

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Albizia chinensis bark extract ameliorates the genotoxic effect of cyclophosphamide

Bulletin of the National Research Centre ◽

10.1186/s42269-020-00422-9 ◽

2020 ◽

Vol 44 (1) ◽

Author(s):

Marian Nabil ◽

Entesar E. Hassan ◽

Neven S. Ghaly ◽

Fawzia A. Aly ◽

Farouk R. Melek ◽

...

Keyword(s):

Folk Medicine ◽

Inhibitory Effect ◽

Protective Role ◽

Bark Extract ◽

Repeated Treatment ◽

Mouse Bone Marrow ◽

Sperm Abnormalities ◽

Wide Range ◽

Different Doses

Abstract Background The genus Albizia (Leguminoseae) is used in folk medicine for the treatment of a wide range of ailments. Recently, saponins from plant origin have attracted much attention. Saponins are recorded to have a broad range of biological and pharmacological activities. This study was performed to evaluate the protective role of Albizia chinensis bark methanolic extract (MEAC) against the genotoxicity induced by cyclophosphamide (CP) using different mutagenic parameters. Results The results showed that MEAC induced an inhibitory effect against chromosomal aberrations of CP in mouse bone marrow and spermatocytes. Such effect was found to be significant (p < 0.01) with a dose of 100 mg/kg treated once for 24 h and also after repeated treatment at a dose of 25 mg/kg for 7 days. In sperm abnormalities, the protective effect of Albizia extract showed a dose-related relationship. Different doses of MEAC (25, 50, and 100 mg/kg) significantly (p < 0.01) ameliorated sperm abnormalities induced by CP dose-dependently. The percentage of sperm abnormalities was decreased to 5.14 ± 0.72 in the group of animals treated with CP plus MEAC (100 mg/kg) indicating an inhibitory effect of about 50%. Conclusion MEAC at the doses examined was non-genotoxic compared to control (negative) and exhibited a protective role against CP genotoxicity.

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Regulation of Cytochrome c Oxidase by Natural Compounds Resveratrol, (–)-Epicatechin, and Betaine

Cells ◽

10.3390/cells10061346 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1346

Author(s):

Icksoo Lee

Keyword(s):

Cytochrome C ◽

Cytochrome C Oxidase ◽

Model Systems ◽

Health Improvement ◽

Published Data ◽

Mitochondrial Electron Transport Chain ◽

Naturally Occurring ◽

Transport Chain ◽

Wide Range ◽

Experimental Model Systems

Numerous naturally occurring molecules have been studied for their beneficial health effects. Many compounds have received considerable attention for their potential medical uses. Among them, several substances have been found to improve mitochondrial function. This review focuses on resveratrol, (–)-epicatechin, and betaine and summarizes the published data pertaining to their effects on cytochrome c oxidase (COX) which is the terminal enzyme of the mitochondrial electron transport chain and is considered to play an important role in the regulation of mitochondrial respiration. In a variety of experimental model systems, these compounds have been shown to improve mitochondrial biogenesis in addition to increased COX amount and/or its enzymatic activity. Given that they are inexpensive, safe in a wide range of concentrations, and effectively improve mitochondrial and COX function, these compounds could be attractive enough for possible therapeutic or health improvement strategies.

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Protective Role of Ice Barriers: How Reproductive Organs of Early Flowering and Mountain Plants Escape Frost Injuries

Plants ◽

10.3390/plants10051031 ◽

2021 ◽

Vol 10 (5) ◽

pp. 1031

Author(s):

Clara Bertel ◽

Jürgen Hacker ◽

Gilbert Neuner

Keyword(s):

Management Strategies ◽

Woody Species ◽

Reproductive Organs ◽

High Elevation ◽

Protective Role ◽

Early Spring ◽

Early Flowering ◽

Ice Formation ◽

Species Vulnerability ◽

Infrared Video

In the temperate zone of Europe, plants flowering in early spring or at high elevation risk that their reproductive organs are harmed by episodic frosts. Focusing on flowers of two mountain and three early-flowering colline to montane distributed species, vulnerability to ice formation and ice management strategies using infrared video thermography were investigated. Three species had ice susceptible flowers and structural ice barriers, between the vegetative and reproductive organs, that prevent ice entrance from the frozen stems. Structural ice barriers as found in Anemona nemorosa and Muscari sp. have not yet been described for herbaceous species that of Jasminum nudiflorum corroborates findings for woody species. Flowers of Galanthus nivalis and Scilla forbesii were ice tolerant. For all herbs, it became clear that the soil acts as a thermal insulator for frost susceptible below ground organs and as a thermal barrier against the spread of ice between individual flowers and leaves. Both ice barrier types presumably promote that the reproductive organs can remain supercooled, and can at least for a certain time-period escape from effects of ice formation. Both effects of ice barriers appear significant in the habitat of the tested species, where episodic freezing events potentially curtail the reproductive success.

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A Novel GIS-Based Approach for Automated Detection of Nearshore Sandbar Morphological Characteristics in Optical Satellite Imagery

Remote Sensing ◽

10.3390/rs13112233 ◽

2021 ◽

Vol 13 (11) ◽

pp. 2233

Author(s):

Rasa Janušaitė ◽

Laurynas Jukna ◽

Darius Jarmalavičius ◽

Donatas Pupienis ◽

Gintautas Žilinskas

Keyword(s):

Satellite Imagery ◽

Morphological Characteristics ◽

Unsupervised Classification ◽

Landsat 8 ◽

Multi Scale ◽

Medium Resolution ◽

Wide Range ◽

Position Index ◽

Local Relief ◽

Length Width

Satellite remote sensing is a valuable tool for coastal management, enabling the possibility to repeatedly observe nearshore sandbars. However, a lack of methodological approaches for sandbar detection prevents the wider use of satellite data in sandbar studies. In this paper, a novel fully automated approach to extract nearshore sandbars in high–medium-resolution satellite imagery using a GIS-based algorithm is proposed. The method is composed of a multi-step workflow providing a wide range of data with morphological nearshore characteristics, which include nearshore local relief, extracted sandbars, their crests and shoreline. The proposed processing chain involves a combination of spectral indices, ISODATA unsupervised classification, multi-scale Relative Bathymetric Position Index (RBPI), criteria-based selection operations, spatial statistics and filtering. The algorithm has been tested with 145 dates of PlanetScope and RapidEye imagery using a case study of the complex multiple sandbar system on the Curonian Spit coast, Baltic Sea. The comparison of results against 4 years of in situ bathymetric surveys shows a strong agreement between measured and derived sandbar crest positions (R2 = 0.999 and 0.997) with an average RMSE of 5.8 and 7 m for PlanetScope and RapidEye sensors, respectively. The accuracy of the proposed approach implies its feasibility to study inter-annual and seasonal sandbar behaviour and short-term changes related to high-impact events. Algorithm-provided outputs enable the possibility to evaluate a range of sandbar characteristics such as distance from shoreline, length, width, count or shape at a relevant spatiotemporal scale. The design of the method determines its compatibility with most sandbar morphologies and suitability to other sandy nearshores. Tests of the described technique with Sentinel-2 MSI and Landsat-8 OLI data show that it can be applied to publicly available medium resolution satellite imagery of other sensors.

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Effects of Long-Wave UV Light on Monilinia Growth and Identification of Species

Plant Disease ◽

10.1094/pdis.1999.83.1.62 ◽

1999 ◽

Vol 83 (1) ◽

pp. 62-65 ◽

Cited By ~ 48

Author(s):

A. De Cal ◽

P. Melgarejo

Keyword(s):

Short Distance ◽

Mycelial Growth ◽

Uv Light ◽

Dark Period ◽

Morphological Characteristics ◽

Potato Dextrose Agar ◽

Long Wave ◽

The World ◽

Identification Of Species ◽

Respective Species

The effect of long-wave UV/dark period on mycelial growth of 46 isolates of Monilinia sp. collected in Spain and 16 isolates collected from other parts of the world was investigated. Typical isolates of M. laxa, M. fructigena, and M. fructicola were grown in the dark and identified by morphological characteristics. Long-wave UV/dark conditions reduced the growth rates of M. laxa, M. fructigena, and M. fructicola on potato dextrose agar. All isolates of M. fructigena grew more slowly than those of M. fructicola. Typical and atypical isolates of M. fructigena and M. fructicola were placed in their respective species based on long-wave UV/dark growth rate data. M. laxa isolates were readily distinguished by the short distance from their conidium to the first germ tube branch. The involvement of different photoreceptors in photoresponses by M. fructicola and M. fructigena is discussed. Differences in mycelial growth under long-wave UV may be a useful tool to identify Monilinia spp.

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Lateral skull base surgery: a complicated pursuit?

The Journal of Laryngology & Otology ◽

10.1017/s0022215107000436 ◽

2007 ◽

Vol 122 (3) ◽

pp. 221-229

Author(s):

V C Cousins

Keyword(s):

Skull Base ◽

Skull Base Surgery ◽

Treatment Modalities ◽

Published Data ◽

Know How ◽

Team Assessment ◽

Pathological Conditions ◽

Lateral Skull Base ◽

Wide Range ◽

Multi Disciplinary Team

AbstractThe management of lesions of the lateral skull base is a highly sophisticated branch of surgery generally performed by otolaryngology–head and neck surgeons as part of a multi-disciplinary team. Assessment of patients with diseases affecting the lateral skull base can be complex, as can the application of the various treatment modalities and the management of the expected and unexpected side effects of that treatment.A wide range of pathological conditions occur in the lateral skull base. Many operations and procedures have been described for dealing with them. There is not necessarily one correct solution to the management of any particular problem in the skull base, with multiple factors to be considered in planning and intervention.As surgeons, we need to know how our own results and outcomes compare with pooled, published data concerning the implications and complications occurring as a result of intervention, in order to better advise our patients on their management.

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Abstract 137: Deficiency of Senescence Marker Protein 30 Exacerbates Doxorubicin-Induced Cardiac Dysfunction in Mice

Circulation Research ◽

10.1161/res.111.suppl_1.a137 ◽

2012 ◽

Vol 111 (suppl_1) ◽

Author(s):

Makiko Miyata ◽

Satoshi Suzuki ◽

Tomofumi Misaka ◽

Shu-ichi Saitoh ◽

Yasuchika Takeishi

Keyword(s):

Cardiac Dysfunction ◽

Protective Role ◽

Left Ventricular ◽

Left Ventricular Ejection ◽

Marker Protein ◽

Morphological Examination ◽

Protective Function ◽

Ventricular Ejection Fraction ◽

Wide Range ◽

Oxidative Effects

Background: Senescence marker protein 30 (SMP30) was originally identified as an aging marker protein in the rat liver. The expression of SMP30 decreases with aging androgen-independently. Doxorubicin (DOX) has been used as a potent anticancer agent, but serious cardiotoxicity precludes its use in a wide range of patients. SMP30 may have anti-oxidative and anti-apoptosis functions in several organs, but functional role of SMP30 has not been rigorously examined in the heart. We hypothesized that SMP30 has cardio-protective function by anti-aging and anti-oxidant effects on DOX-induced cardiac dysfunction. Method and Results: Male SMP30 knockout (SMP30KO) and age-matched littermate male wild-type (WT) mice at 12-14 weeks of age were given intraperitoneal injections of DOX (20 mg/kg) or saline. Seven days after DOX injection, echocardiography revealed that left ventricular ejection fraction in DOX-treated SMP30KO mice was more severely reduced than in DOX-treated WT mice (40.9 ± 3.1% vs. 46.9 ± 4.9%, P<0.01). Morphological examination of myocardial sections showed fibrotic change in DOX-treated SMP30KO mice significantly increased compared to DOX-treated WT mice (3.2 ± 0.5% vs. 1.3 ± 0.2%, P<0.01). Generation of reactive oxygen species assessed by dihydroethidium staining was greater in DOX-treated SMP30KO mice than DOX-treated WT mice (166.9 ± 8.6% vs. 131.6 ± 5.8%, P<0.01). Moreover, apoptotic signaling pathways such as caspase-3 activity (1.8 ± 0.1% vs. 1.1 ± 0.2%, P<0.01), bax/bcl-2 ratio (2.4 ± 0.3% vs.1.6 ± 0.2%, P<0.05) and phosphorylation activity of c-Jun N-terminal kinase (1.6 ± 0.3 vs. 1.0 ± 0.1, P<0.05) were significantly elevated in the SMP30KO mice compared with WT mice after DOX injection. The numbers of TUNEL-positive nuclei in the myocardium were higher in DOX-treated SMPKO mice than in DOX-treated WT mice (0.15 ± 0.02% vs. 0.08 ± 0.01%, P<0.01). Conclusions: The results of this study demonstrated that DOX-induced cardiotoxicity is aggravated in SMP30KO mice by exacerbating of superoxide generation, leading to enhanced apoptosis of cardiomyocytes. SMP30 has a cardio-protective role by anti-apoptotic and anti-oxidative effects in DOX-induced cardiotoxicity.

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