scholarly journals Chemical Compound of Terminalia Catappa L. as Hemostatic Agents in Post Tooth Extraction

2021 ◽  
Vol 4 (4) ◽  
Author(s):  
Ni Made Sirat ◽  
◽  
Asep Arifin Senjaya
Keyword(s):  
Antioxidant Activity ◽  
Tooth Extraction ◽  
Activity Index ◽  
Water Extract ◽  
Tree Height ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Distilled Water ◽  
Terminalia Catappa ◽  
Herbal Plants

Terminalia catappa L. is one of the herbal plants that contain flavonoids that play a role in wound healing including to bleeding after tooth extraction. The aim of our study was to identify the chemical compounds contained in Terminalia catappa L. leaves. This type of research was experimental. The sample in this study was the leaves of Terminalia catappa number 3-6 from the base, at a tree height of 6 meters picked as much as 6 kilograms. Data analysis qualitatively and quantitatively. The highest content in ethanol extract of Terminalia catappa L of saponins and alkaloids were fractioned with distilled water and the lowest content were fractioned with hexane. Meanwhile distilled water is also used to fractioned the highest content of tannins and phenol. While ethyl acetate was used to fractioned the lowest content of tannins and the highest content of flavonoid. The lowest flavonoid in distilled water extract was fractioned using hexane. For the lowest content of phenol was fractioned using hexane. The results show the highest content of saponins is 3,787.80 mg/100g, the lowest is 166.67 mg/100g. The highest content of Alkaloids is 1,798.57 mg/100g, and the lowest is 576.80 mg/100g. The highest content of tannins is 53,140.72 mg/100g, the lowest is 8,391,803 mg/100g. The highest content of flavonoids is 2,5964.14 mg/100g, the lowest flavonoid is 462.84 mg/100g. The highest content of phenol 29,968.05 mg/100g, the lowest is 225.46 mg/100g. The highest antioxidant activity with AAI (Antioxidant Activity Index) obtains value of 0.36. This shows the moderate antioxidant ability. Terminalia catappa L. contain saponins, alkaloids, tannins, flavonoids, and triterpenoids. The active compound of Terminalia catappa L will generally be produced optimally if a polar solvent is used.

scholarly journals Chemical Compound of Terminalia Catappa L. as Hemostatic Agents in Post Tooth Extraction

2021 ◽  
Author(s):  
Ni Made Sirat ◽  
Asep Arifin Senjaya
Keyword(s):  
Antioxidant Activity ◽  
Tooth Extraction ◽  
Activity Index ◽  
Water Extract ◽  
Tree Height ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Distilled Water ◽  
Terminalia Catappa ◽  
Herbal Plants

Terminalia catappa L. is one of the herbal plants that contain flavonoids that play a role in wound healing including to bleeding after tooth extraction. The aim of our study was to identify the chemical compounds contained in Terminalia catappa L. leaves. This type of research was experimental. The sample in this study was the leaves of Terminalia catappa number 3-6 from the base, at a tree height of 6 meters picked as much as 6 kilograms. Data analysis qualitatively and quantitatively. The highest content in ethanol extract of Terminalia catappa L of saponins and alkaloids were fractioned with distilled water and the lowest content were fractioned with hexane. Meanwhile distilled water is also used to fractioned the highest content of tannins and phenol. While ethyl acetate was used to fractioned the lowest content of tannins and the highest content of flavonoid. The lowest flavonoid in distilled water extract was fractioned using hexane. For the lowest content of phenol was fractioned using hexane. The results show the highest content of saponins is 3,787.80 mg/100g, the lowest is 166.67 mg/100g. The highest content of Alkaloids is 1,798.57 mg/100g, and the lowest is 576.80 mg/100g. The highest content of tannins is 53,140.72 mg/100g, the lowest is 8,391,803 mg/100g. The highest content of flavonoids is 2,5964.14 mg/100g, the lowest flavonoid is 462.84 mg/100g. The highest content of phenol 29,968.05 mg/100g, the lowest is 225.46 mg/100g. The highest antioxidant activity with AAI (Antioxidant Activity Index) obtains value of 0.36. This shows the moderate antioxidant ability. Terminalia catappa L. contain saponins, alkaloids, tannins, flavonoids, and triterpenoids. The active compound of Terminalia catappa L will generally be produced optimally if a polar solvent is used.

Identification of Chemical Compounds from Artemisia gmelinii using UPLC-QTOF-MS/MS and their Regulatory Effects on Immune Responses in DSS-Induced Colitis Mice

2021 ◽  
pp. 1-23
Author(s):  
Yang-Ju Son ◽  
Ji Min Shin ◽  
In Jin Ha ◽  
Saruul Erdenebileg ◽  
Da Seul Jung ◽  
...  
Keyword(s):  
Immune Responses ◽  
High Performance ◽  
Activity Index ◽  
Disease Activity Index ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Chronic Inflammatory Disease ◽  
Lymphoid Tissues ◽  
Food Ingredient ◽  
Cytokines And Chemokines

Artemisia gmelinii Web. ex Stechm. (AG), a popular medicinal herb in Asia, has been used as a common food ingredient in Korea and is traditionally known for its anti-inflammatory properties. Therefore, in this study, we aimed to investigate whether AG relieves IBD, a classic chronic inflammatory disease of the gastrointestinal tract. We identified 35 chemical compounds in AG ethanol extract using ultra-high-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry. In mice with DSS-induced IBD, AG administration attenuated the disease activity index and the serum and colonic levels of inflammatory cytokines and chemokines. AG treatment decreased nuclear factor-[Formula: see text]B (NF-[Formula: see text]B) signaling, a key mediator of inflammation, in the mouse colons. Additionally, AG extract enhanced immune responses in lymphoid tissues such as spleen and Peyer’s patches. Thus, AG consumption potently ameliorated IBD symptoms and improved immune signaling in lymphoid tissues.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN SENYAWA FLAVONOID PADA KULIT BATANG TUMBUHAN GAYAM (Inocarpus fagiferus Fosb.)

Jurnal Kimia ◽  
2016 ◽  
Author(s):  
Made Hany Anastasia ◽  
Sri Rahayu Santi ◽  
Manuntun Manurung
Keyword(s):  
Antioxidant Activity ◽  
Ammonium Thiocyanate ◽  
Water Extract ◽  
Lipid Peroxide ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Chloroform Extract ◽  
Hydroxyl Groups ◽  
Tropical Plant ◽  
Dpph Method

Gayam (Inocarpus fagiferus Fosb) is a tropical plant whose stem bark has been used as traditional medicine. This research aims to identify the flavonoides isolate from stem bark of gayam and identify the class of flavonoids compound as antioxidant. Extraction of 1000 g stem bark powder with 8500 mL of 96 % ethanol to yield 30 g concentrated ethanol extract. Partition of 15 g extract ethanol-water (7:3) with chloroform and n-buthanol to yield 1,5 g concentrated chloroform extract, 5,87 g concentrated n-buthanol extract and 5,63 g concentrated water extract. The flavonoids test using Wilstatter and Bate-Smith Matcalf reagent showed that n-buthanol and water extract contain flavonoids compounds. Antioxidant activity of flavonoids tested with DPPH method and lipid peroxide ammonium thiocyanate method showed that n-buthanol extract had antioxidant activity with IC50 of 24 ppm and n-buthanol extract has ability to inhibit the formation of lipid peroxide with inhibition value of 63,04 %. Separation of n-buthanol extract using thin layer chromatography preparative with chloroform as mobile phase and silica gel GF254 as stationary phase obtained four fractions. Flavonoids test showed that fraction F3 contained flavonoids compounds. FTIR analysis indicated that isolate had functional groups such as  -OH , C=O, C-O alcohol, C=C aromatic, C-H aromatic and C-H aliphatic. UV-vis spectra showed 2 peaks at ? 310 nm  and  261 nm which indicated flavonoids group of  isolfavone with hydroxyl groups at C-5 and  C-7. Antioxidant activity test with DPPH method showed that the isolate had antioxidant activity with IC50 of 1 ppm.

scholarly journals Toksisitas Ekstrak Etanol dan Ekstrak Air dari Daun Jotang Kuda (Synedrella nodiflora (L.) Gaertn.), Daun Gandarusa (Justicia Gendarussa Burm.F.), dan Daun Pulutan (Urena lobata L.) dengan Brine Shrimp Lethality Test

2019 ◽  
Vol 5 (2) ◽  
pp. 198-205
Author(s):  
Agustinus Widodo ◽  
Akhmad Khumaidi ◽  
Putri Faradila A. Lasongke
Keyword(s):  
Thin Layer ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Water Extract ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Test Results ◽  
Synedrella Nodiflora ◽  
Central Sulawesi ◽  
Layer Chromatography

Jotang kuda (Synedrella nodiflora (L.) Gaertn.), gandarusa (Justicia gendarussa Burm.f.) and pulutan (Urena lobata L.) leaves are used by several tribes in Central Sulawesi for the treatment of cancer. This study aims to determine the value of LC50 (Lethal Concentration 50) of ethanol and water extracts from the S. nodiflora, J. gendarussa and U. lobata leaves and identify the class of chemical compounds contained in extracts with the highest toxicity. The ethanol extract was obtained by the maceration method using ethanol 96% and the water extract was obtained by the infusionmethod. The extract toxicity test was carried out byBrine Shrimp Lethality Test (BSLT) and dentification test was carried out by Thin Layer Chromatography (TLC). The toxicity test results (LC50) of 96% ethanol extract and water extract of S. nodiflora leaves were 395.60 μg/ml and 109.25 μg/ml; J. gendarussa leaves 713.34 μg/ml and 18.02 μg/ml; and U.lobata leaves 188.38 μg/ml and 85.37 μg/ml, respectively. The results of identification showed that the water extract of the J. gendarussa leaves containing alkaloids, flavonoids, phenolics, saponins, and steroids-terpenoids. The results of this study indicated that the extracts of S. nodiflora leaves, J. gendarussa leaves, and U. lobata leaves are potential to be developed as anticancer.

scholarly journals Antioxidant activities and simultaneous HPLC-DAD profiling of polyphenolic compounds from Moringa oleifera Lam. Leaves grown in Bangladesh

Food Research ◽  
2021 ◽  
Vol 5 (1) ◽  
pp. 401-408
Author(s):  
K.S. Ahmed ◽  
I.A. Jahan ◽  
F. Jahan ◽  
H. Hosain
Keyword(s):  
Antioxidant Activity ◽  
Moringa Oleifera ◽  
Water Extract ◽  
Ethanol Extract ◽  
Polyphenolic Compounds ◽  
Dihydroxybenzoic Acid ◽  
High Concentration ◽  
Dry Extract ◽  
Mature Leaves ◽  
Moringa Oleifera Lam

Moringa oleifera Lam. has been used as a traditional medicine for the treatment of several diseases. In this study, the antioxidant activity and simultaneous HPLC profiling of polyphenolic compounds in 80% ethanol and water extracts of M. oleifera tender and mature leaves available in Bangladesh were investigated. The 80% ethanol extract of M. oleifera tender leaves contained high concentration and more amount of 3,4- dihydroxybenzoic acid, (-) epicatechin and rosmarinic acid (201.32±1.94, 213.08±1.96 and 133.84±1.42 mg/100 g of dry extract, respectively) than that of mature leaves (82.55±1.09, 141.86±1.10 and 16.23±0.76 mg/100 g of dry extract, respectively). On the other hand, the water extract of M. oleifera tender leaves contained a high concentration of catechin hydrate, trans-ferulic acid and quercetin (530.05±1.91, 166.38±0.90 and 771.74±1.34 mg/100 g of dry extract, respectively) than that of mature leaves (207.21±0.90, 62.42±0.45 and 483.25±1.04 mg/100 g of dry extract, respectively). It was also found that 3,4-dihydroxybenzoic acid, catechol, p-coumaric acid and trans-cinnamic acid were detected only in 80% ethanol extract of M. oleifera leaves but syringic acid was identified in the water extract. In DPPH free radical scavenging activity test, IC50 values of 80% ethanol extract of tender leaves (EETL), 80% ethanol extract of mature leaves (EEML), water extract of tender leaves (WETL) and water extract of mature leaves (WEML) were found to be moderately significant (105.50±1.05, 115.00±1.14, 216.00±1.34 and 269.50±1.56 μg/mL, respectively) when compared to the IC50 value of ascorbic acid, 3.32±0.07 μg/mL. The polyphenolic compounds are responsible for antioxidant activity. On the basis of the present study, it can be suggested that the leaves show antioxidant activity and it’s contained a significant amount and different types of polyphenolic compounds. Standardized polyphenolic compounds from M. oleifera leaves could be used as a better source of natural antioxidant supplement.

scholarly journals Formulation, Physical Quality Evaluation, and Antioxidant Activity of Body Butter of Ethanol Extract of Dragon Fruit (Hylocereus polyrhizus) Peel

2020 ◽  
Vol 25 (3) ◽  
Author(s):  
Agustina Nila Yuliawati ◽  
Kadek Duwi Cahyadi
Keyword(s):  
Antioxidant Activity ◽  
Quality Evaluation ◽  
Activity Index ◽  
Ethanol Extract ◽  
The Body ◽  
Fruit Peel ◽  
Physical Quality ◽  
Antioxidant Content ◽  
Dragon Fruit ◽  
Hylocereus Polyrhizus

Dragon fruit (Hylocereus polyrhizus) has polyphenols as an antioxidant. It has known that the antioxidant content of dragon fruit peels was more than in the flesh, so it can be used as a source of natural antioxidants to replace synthetic antioxidants. The use of dragon fruit (Hylocereus polyrhizus) peel, especially as topical preparations in the form of body butter, was still rarely done, whereas dragon fruit peel as an antioxidant can be used as an active ingredient of cosmetics. The purpose of this study was to obtain the body butter formula of ethanol extract of dragon fruit (Hylocereus polyrhizus) peel and its physical quality evaluation, to know the antioxidant activity of ethanol extract of dragon fruit (Hylocereus polyrhizus) peel and its body butter. This research was an experimental study with the stages of research consisting of determination of native plants, making ethanol extract of dragon fruit (Hylocereus polyrhizus) peel, ensuring its activity antioxidant, performing body butter formulation procedures, carrying out physical quality evaluation such as organoleptic, homogeneity, pH, spread, protection, and adhesion ability, then antioxidant activity of its body butter. The result of this research showed that the ethanol extract of dragon fruit (Hylocereus polyrhizus) peel has a moderate level of antioxidant (Antioxidant Activity Index / AAI = 0,88). Furthermore, body butter which has contains antioxidant content of ethanol extract of dragon fruit (Hylocereus polyrhizus) peel as much as 0.5% has the best physical quality evaluation during storage and the highest AAI (0,54) among other body butter formulas.

scholarly journals AKTIVITAS ANTIOKSIDAN DAN IDENTIFIKASI SENYAWA EKSTRAK JAMUR LINGZHI (Ganoderma lucidum) DENGAN LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY (LC-MS)

EKOLOGIA ◽  
2019 ◽  
Vol 19 (2) ◽  
pp. 65-72
Author(s):  
Farida Nuraeni ◽  
Septi Bernadetha Br Sembiring
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Time Variation ◽  
Ganoderma Lucidum ◽  
Water Extract ◽  
Ethanol Extract ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Dpph Method

Lingzhi mushroom is widely used as an alternative treatment to reduce blood pressure and blood sugar levels, to maximize the potential of the Lingzhi fungus antioxidant tests are carried out. This study aims to determine the potential antioxidant activity of water extract and 70% ethanol extract of Lingzhi mushroom with maceration extraction time variation and identification of compounds with Liquid Chromatography Mass Spectrometry (LC-MS). This research begins with the determination of fresh lingzhi mushrooms then made simplicia and extracted by maceration with time variation with soaking time of 24 hours, 48 hours and 72 hours each with 2 solvents namely water and 70% ethanol. The extract was tested by phytochemistry followed by testing the antioxidant activity of Lingzhi mushroom extract (ganoderma lucidum) by DPPH method. Then the compounds were identified by Liquid Chromatography-Mass Spectrometry (LC-MS). Based on the results of research that maceration for 1 hour (1 day) Lingzhi mushroom with 70% ethanol extraction has the potential as an antioxidant with an IC50 value of 94.83 ppm. The results of identification with LC-MS in 70% ethanol extract as a compound that has potential as an antioxidant are Bisphenol M compounds, and 1- - [[2- (3,4-Dimethoxyphenyl) amino]} -3-methyl-2-octylpyrido [1 , 2-a] benzimidazole-4-carbonitrile.

scholarly journals Comparative Study on Phenolic Compounds and Antioxidant Activities of Hop (Humulus lupulus L.) Strobile Extracts

Plants ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 135
Author(s):  
Jae Il Lyu ◽  
Jaihyunk Ryu ◽  
Kyoung-Sun Seo ◽  
Kyung-Yun Kang ◽  
Sang Hoon Park ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phenolic Compound ◽  
Antioxidant Activities ◽  
Water Extract ◽  
Ethanol Extract ◽  
Antioxidant Property ◽  
Flavonoid Compound ◽  
Total Phenolic ◽  
Extraction Solvent

In this study, we investigated the phenolic compounds in hop strobile extracts and evaluated their antioxidant property using DPPH and ABTS assay. The total phenolic compound (TPC) and total flavonoid compound (TFC) estimated in two different solvent extracts considerably varied depending on the extraction solvent. The most abundant phenolic compound in hop strobile was humulones (α-acid) with levels ranging from 50.44 to 193.25 µg/g. El Dorado accession revealed higher antioxidant activity in ethanol extracts (DPPH: IC50 124.3 µg/mL; ABTS: IC50 95.4 µg/mL) when compared with that of the other accessions. Correlations between DPPH (IC50) scavenging TFC in ethanol extract (TFC_E, −0.941), and TPC_E (−0.901), and between ABTS (IC50) scavenging TFC_E (−0.853), and TPC_E (−0.826), were statistically significant at p < 0.01 level, whereas no significant correlation was observed between antioxidant activities, TPC and TFC in water extract. This study is the first to report that variations in the level of phenolic contents and antioxidant activity of various hop cultivars depended on the type of extraction solvent used and the cultivation regions. These results could provide valuable information on developing hop products.

scholarly journals Antioxidant activity of Trachystemon orientalis (L.) G. Don (Borage) grown and eaten as food in Ordu, Turkey

2015 ◽  
Vol 61 (4) ◽  
pp. 40-51 ◽  
Author(s):  
Melek Col Ayvaz
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Total Antioxidant Capacity ◽  
Water Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Synthetic Antioxidants ◽  
Total Antioxidant ◽  
Phenolic And Flavonoid

SummaryIntroductıon: Instead of synthetic antioxidants, using of natural products with antioxidant activity is demanded.Objectıve: The aim of this study is to evaluate the antioxidant activity and total phenolic and flavonoid contents of theTrachystemon orientalis(borage) obtained from Ordu.Methods: The water and ethanol extracts of the borage plant were investigated by using severalin vitroantioxidant activity tests such as total antioxidant capacity, O2•−,•OH, DPPH and ABTS radicals scavenging activities, hydrogen peroxide scavenging activity, FRAP, Fe2+chelating and reducing power assays in order to make effective comparisons.Results: Water extract of theT. orientalishad the more phenolic (90 mg GA/g extract) and flavonoid (56.88 mg CT/g extract) contents than ethanol extract. Furthermore, both of the extracts showed strong antioxidant and radical scavenging activities determined by differentin vitromodels. The highest total antioxidant capacity as ascorbic acid equivalents of 244.50 mg/g extract was also calculated for water extract. Generally, a relationship between total phenolic content and antioxidant activity was established. It is also an important finding for an edible food source that the phenolic and flavonoid content and antioxidative activities for water extract was higher than the ethanol extract.Conclusıon:T. orientaliscould be used as a food ingredient instead of synthetic antioxidants and all results will contribute to the recent increase in investigations on using natural products in many areas such as food, pharmacy, alternative medicine and natural therapy.

scholarly journals Protective effect of medicinal fungus Xylaria nigripes mycelia extracts against hydrogen peroxide-induced apoptosis in PC12 cells

2017 ◽  
Vol 30 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Rupesh D Divate ◽  
Pei-Ming Wang ◽  
Chiun-Chuang Wang ◽  
Su-Tze Chou ◽  
Chen-Tien Chang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Pc12 Cells ◽  
Water Extract ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Hot Water ◽  
Neuroprotective Effects ◽  
Medicinal Fungus ◽  
Induced Apoptosis

Xylaria nigripes ( XN) is a medicinal fungus that was used traditionally as a diuretic, nerve tonic, and for treating insomnia and trauma. In this study, we elucidated possible mechanisms of neuroprotective effects of XN mycelia extracts. XN mycelia were produced by fermentation. Hot water extract and 70% ethanol extract of XN mycelia were evaluated on hydrogen peroxide (H2O2)-induced apoptosis in PC12, a rat pheochromocytoma cell line. Both XN extracts effectively protected PC12 cells against H2O2-induced cell damage by inhibiting release of lactate dehydrogenase, decreasing DNA damage, restoring mitochondrial membrane potential, and arresting abnormal apoptosis through upregulation of Bcl-2 and downregulation of Bax and caspase 3. Compared to water extract, ethanol extract showed not only greater neuroprotective effects but also a higher antioxidant activity by scavenging DPPH radicals, inhibiting lipid peroxidation, and reducing power. High phenolic content and antioxidant activity may provide the neuroprotective properties of XN ethanol extract.

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