Estimation of Bioactive Compound of Catharanthus Roseus Leaf Extract by Phytochemical Screening and GC-MS Analysis

The investigation was carried out to determine the possible phytochemical constituents from aqueous, methanol and chloroform extracts of Turnera subulata leaf extracts. Among the phytochemical screening of these extracts, Methanolic extract showed that the leaf was rich in alkaloids, flavonoids, glycosides, phenols, saponins and quinones. The chemical composition of the plant leaf extract of T. subulata was investigated using Gas Chromatography – Mass Spectroscopy (Agilent-7890A GC instrument coupled with MS-5975) and NIST-MS library. GC-MS analysis of T. subulata plant leaf extract, revealed the existence of the GC-MS chromatogram of the major peaks presented in methanolic extract like Methyl 8,11,14-heptadecatrienoate (23.244%), Pentadecanoic acid, 14-methyl-,methyl ester (8.654%), n-Hexadecanoic acid (8.654%), 4H-Pyran4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl (6.598%), 1b,4a-Epoxy-2H-cyclopenta[3,4] cyclopropa[8,9]cycloundec[1,2-b] oxiren-5(1aH)-one(5.400%), 3,7,11,15-Tetramethyl-2-hexadecen-1- ol(5.400%), etc. From this study it is obvious that T. subulata leaf extract contains many biologically active compounds and also it gives a detailed insight about the phytochemical profile which could be exploited for the development of plant based drug.

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GC-MS Analysis and Phytochemical Screening of Indigofera tinctoria (Linn.) Leaf Extract Characterizing its Medicinal Use

International Journal of Ayurvedic Medicine ◽

10.47552/ijam.v11i2.1540 ◽

2020 ◽

Vol 11 (2) ◽

pp. 289-299

Author(s):

Mishra D N ◽

Gomare K S ◽

Sheelwant S V

Keyword(s):

Ethanol Extract ◽

Bioactive Compound ◽

Gas Chromatography Mass Spectrometry ◽

Protein Synthesis Inhibitor ◽

Human Beings ◽

Phytochemical Screening ◽

Synthesis Inhibitor ◽

Activity Data ◽

Ms Analysis ◽

Indigofera Tinctoria

Indigofera tinctoria (Linn.), a perennial shrub belongs to the family Fabaceae (Leguminosae) is well known in Indian system of medicines for its traditional uses. The present study was carried to find out the possible bioactive components in the leaves of this plant in ethanol extract by Gas Chromatography-Mass Spectrometry (GC-MS) analysis and the therapeutic uses of each compound as per PASS and other database. The mass spectra of the extract found compounds that were matched with the National Institute of Standards and Technology (NIST version 5.7.1) library database. The GC-MS study observed different peaks as per retention time and then those peaks were analysed determining presence of 26 phytochemical compounds. The qualitative phytochemical screening done by routine laboratory methods also indicated presence of these bioactive compound groups like alkaloids, flavonoids, tannins and phenols, saponins, glycosides and terpenoids. These 26 compounds are understood to have different therapeutic activities as per in-silico databases, e.g. 2-Acetylamino-3-hydroxy-propionoic acid (glutamic acid) - used as Anti-diarrheal, Antiviral, Antipyretic, Protein synthesis inhibitor, Antidiabetic, Non-steroidal, Anti-inflammatory agent, Antipsoriatic, Antioxidant, Antifungal, Antineoplastic (sarcoma), Antiparasitic, Antibiotic Aminoglycoside-like, Antiseborrheic, Anticataract, Antithyroid, Anticarcinogenic, Antileprosy, Hair growth stimulant, Lipoprotein lipase inhibitor, Dermatologic, Testosterone, Antituberculosic, Antirickettsial, Antianemic, etc. activities. Hence, Indigofera tinctoria is an excellent source of phytocompounds, which help to heal various diseases and health complications in human beings. The use of this plant in traditional methods for the treatment of such diseases appears scientifically relevant as per the therapeutic activity data of its bioactive compounds.

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Phytochemical Screening and GC-MS Analysis of Cardiospermum halicacabum L. Leaf Extract

International Journal of Trend in Scientific Research and Development ◽

10.31142/ijtsrd15849 ◽

2018 ◽

Vol Volume-2 (Issue-5) ◽

pp. 512-516

Author(s):

S. Nathiya ◽

B. Senthil Kumar ◽

K. Devi ◽

Keyword(s):

Leaf Extract ◽

Phytochemical Screening ◽

Ms Analysis

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PHYTOCHEMICAL SCREENING AND LARVICIDAL EFFECT EVALUATION OF AZADIRACHTA INDICA (L) LEAF EXTRACT ON DERMESTES MACULATUS (DE GEER, 1774) INFESTATION OF SMOKED CLARIAS GARIEPINUS (BURCHELL, 1822)

FUDMA Journal of Sciences ◽

10.33003/fjs-2020-0402-89 ◽

2020 ◽

Vol 4 (2) ◽

pp. 41-45

Author(s):

Sadam Sulaiman Indabo ◽

Rufai Zakari

Keyword(s):

Azadirachta Indica ◽

Leaf Extract ◽

Standard Procedure ◽

Clarias Gariepinus ◽

Phytochemical Screening ◽

Range Finding ◽

Dermestes Maculatus ◽

Randomized Design ◽

Fish Samples ◽

Initial Source

Dermestes maculatus is a major pest of stored fish in Nigeria, fish is preserved with highly persistent synthetic chemicals. There have been an increasing effort at developing plant-based toxicants that are environmentally friendly. Therefore, this study was conducted to screen the leaf extract of Azadirachta indica for phytochemical constituents. The efficacy of the methanolic leaf extract of the plant against D. maculatus was also evaluated. The leaves of A. indica were obtained, processed and taken to laboratory for methanolic extraction. Phytochemical screening was carried out to identify saponins, flavonoids, alkaloids, tannins, steroids, cardiac glycosides, glycosides, triterpenes and carbohydrates using standard procedure. Both clean and infested samples of smoke-dried Clarias gariepinus were purchased from Sabon Gari Market, Zaria. The infested samples were used as initial source of D. maculatus larvae. Range finding test was carried out prior to the bioassay. During the bioassay, clean un-infested fish samples were weighed and introduced into kilner jars and respective dosages of 0.2g, 0.4g, 0.6g and 0.8g of extract were added. Another set of fish samples without the extract were maintained as control. The experiment was arranged in Completely Randomized Design with three replicates. Fifteen larvae of D. maculatus were introduced into both treated and control containers and covered. Mortality was recorded after 24, 48, 72 and 96 hours. Data obtained revealed an increase in mortality with increasing dose and time. Mortality ranged from 1.67 to 11.00. Therefore, leaf extract of A. indica should be incorporated in the development of larvicide against D. maculatus.

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Enhanced Anticancer Activity and Apoptosis Effect of Bioactive Compound Quercetin Extracted from Ocimum sanctum Leaves

Current Nanomedicine ◽

10.2174/2468187310999201117155414 ◽

2020 ◽

Vol 10 ◽

Author(s):

Amutha Santhanam ◽

Naveen Kumar Chandrasekharan ◽

Rajangam Ilangovan

Keyword(s):

Cell Cycle ◽

Anticancer Activity ◽

Cancer Cells ◽

Leaf Extract ◽

Nickel Nanoparticles ◽

Bioactive Compound ◽

Cell Cycle Analysis ◽

Ocimum Sanctum ◽

Sem Images ◽

Ft Ir

Background: The occurrence of Cancer results in cellular changes that causes the uncontrolled growth and division of cells. Apoptosis removes cells during development and eliminates the potentially cancerous cells. The bioactive compounds present in the herbal plant shows cytotoxic activity that result in apoptosis. The traditional herbal plants are used world-wide both in allopathy and other traditional ways. Objective: The main objective of this study is to extract the bioactive compound Quercetin from the medicinally significant plant Ocimum sanctum and also to develop nanomedicine as Qu-PEG-NiGs. Materials and Methods: Leaf extract of the medicinally significant plant Ocimum sanctum (O. sanctum) has been used for the synthesis of nickel nanoparticles (NiGs) and extraction of quercetin (Qu). The ethanolic extract of Ocimum sanctum is added to 1 mM Nickel Nitrate (Ni(NO3)2) and stirred for 3 hrs at RT and dried at 60°C for 3hrs and calcinated at 400°C for 2hrs and characterized using Uv-Vis Spectrophotometer, FT-IR, SEM, DLS and Zeta potential. The Quercetin is isolated from Ocimum sanctum leaf extract using the reflux condenser method. The bio-polymer is being PEG-coated over NiGs and Quercetin is loaded into it. The apoptosis activity using MCF-7 cells is performed with Qu-PEG-NiGs. The purity of Quercetin is characterized using HPLC. In order to analyse apoptosis efficiency, MTT assay, Reactive Oxygen Species (ROS), Cell cycle analysis has been performed. Results: The NiGs absorption spectrum gives a peak at 408nm. The FT-IR confirms the presence of particular functional groups shifting from the compound NiGs and then coated with PEG-Qu-NiGs. The SEM images show the size of NiGs ranging from 27.3 nm to 40.4 nm with varied morphology such as hexagonal and other irregular shapes. The presence of Quercetin extracted from the leaf powder is approximately 1.5 mg/g. The ROS results show the Qu-PEG-NiGs induced efficiency of the apoptosis, while the increased concentrations promote ROS and lead to activation of the apoptosis. The cell cycle analysis has shown the cytotoxic effect. Conclusion: PEG-coated nickel nanoparticles can be used as a promising chemotherapeutic agent against MCF7 breast cancer cells. It is the evidence to further studies for evaluating Qu-PEG-NiGs anticancer activity on different types of cancer cells.

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In vitro anti-bacterial activity of Tinospora cordifolia leaf extract and its phytochemical screening

Journal of Biomedical Sciences ◽

10.3126/jbs.v5i2.23633 ◽

2019 ◽

Vol 5 (2) ◽

pp. 10-17

Author(s):

B. Praiwala ◽

S. Priyanka ◽

N. Raghu ◽

N. Gopenath ◽

A. Gnanasekaran ◽

...

Keyword(s):

Cell Division ◽

Leaf Extract ◽

Antibacterial Property ◽

Standard Procedure ◽

Tinospora Cordifolia ◽

Diffusion Method ◽

Main Concern ◽

Phytochemical Screening ◽

Leaf Extracts ◽

Screening Assay

Background: Antimicrobial resistance is the main concern worldwide to combat infectious. Over the years studies on leaf extracts Tinospora cordifolia have demonstrated the potent role its antibacterial property. The current study is an attempt to test its antibacterial property against Escherichia coli cell division. Material and methods: Phytochemical screening assay of T. cordifolia leaf extract was done using standard procedure and the results showed the presence of alkaloid, carbohydrate, terpenoid, steroid, tannin, amino acid, flavonoid and glycoside components. Results: HPLC analysis revealed the presence of berberine in T. cordifolia leaf extract. Further E. coli cells were treated with berberine to study its efficacy in inhibiting cell division. Antibacterial assay was performed by using disc diffusion method. Conclusion: Among aqueous, methanolic, ethanolic, chloroform, hexane and acetone extract only methanolic extract showed zone of inhibition.

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Phytochemical screening of various extracts of leaves of Stereospermum personatum (Hassk.) Chatterjee with a focus on GC-MS analysis of bioactive compounds in methanolic extract

Asian Journal of Pharmaceutical and Health Sciences ◽

10.5530/ajphs.2021.11.15 ◽

2021 ◽

Vol 11 (3) ◽

pp. 2507-2511

Author(s):

Indira Indira G ◽

Padmaja Padmaja V

Keyword(s):

Bioactive Compounds ◽

Methanolic Extract ◽

Phytochemical Screening ◽

Ms Analysis

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BIOSYNTHESIZED SILVER NANOPARTICLES USING CATHARANTHUS ROSEUS AND THEIR ANTIBACTERIAL EFFICACY IN SYNERGY WITH ANTIBIOTICS; A FUTURE ADVANCEMENT IN NANOMEDICINE

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2021.v14i2.39856 ◽

2021 ◽

pp. 116-124

Author(s):

MONIKA GUPTA

Keyword(s):

Electron Microscopy ◽

Silver Nanoparticles ◽

Catharanthus Roseus ◽

Leaf Extract ◽

Research Work ◽

Resistant Bacteria ◽

Surface Plasmon Resonance Peak ◽

Plasmon Resonance Peak ◽

X Ray ◽

Uniform Dispersion

Objective: This research work develops an approach to synthesize silver nanoparticles (AgNPs) by reduction of leaf extract of Catharanthus roseus plant. This study produces synthesized nanoparticles that have process-controlled attributes which make their antibiotic action highly efficient. These attributes include smaller size, proper morphology, uniform dispersion, metal ion content, and formation of functional groups. By optimizing the reduction process parameters, AgNPs gain the desired properties. Methods: The biosynthesis of AgNPs process was performed using reaction of 10% (w/v) C. roseus leaf extract with AgNO3. The optimum conditions and concentration used for synthesis of nanoparticles were: 1 mM AgNO3, pH 5, and temperature 80°C with an incubation time of 72 h. All the above parameters were analyzed by ultraviolet-visible spectrophotometer with the surface plasmon resonance peak obtained at 440 nm. Results: Various characterization techniques were performed, namely, scanning electron microscopy, energy-dispersive X-ray, transmission electron microscopy, photoluminescence study, X-ray diffraction spectroscopy, Fourier transform infrared, dynamic light scattering, and atomic force microscopy. The results obtained from characterization confirmed the spherical morphology of the nanoparticles with size between 50 and 87 nm. In the current investigation, the antimicrobial activity of biosynthesized AgNPs was also determined using minimum inhibitory concentration and zone of inhibition methods against six different bacteria at different doses of AgNPs (100, 150, and 200 μg/ml) alone and also in combination with antibiotic-streptomycin. Conclusion: The results revealed that high concentration of AgNPs inhibits the bacterial growth. Furthermore, AgNPs revealed much stronger antibacterial action in synergy with streptomycin against antibiotic-resistant bacteria.

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Phytochemical Screening, GC-MS Analysis and Antioxidant Activity of CurcurbitapepoL. usingIts Leaf Sample

Asian Journal of Biology ◽

10.9734/ajob/2021/v11i430151 ◽

2021 ◽

pp. 33-45

Author(s):

Ezekwe Ahamefula Sunday ◽

Nwadike Constance Nnedimma ◽

Wokocha Gift Peter ◽

George Boma Orlando

Keyword(s):

Mass Spectrometry ◽

Molecular Weight ◽

Antioxidant Activity ◽

Gas Chromatography Mass Spectrometry ◽

State University ◽

Phytochemical Screening ◽

Leaf Sample ◽

Standard Methods ◽

Ms Analysis ◽

Ethylhexyl Phthalate

This study evaluated the phytochemical screening, gas chromatography-mass spectrometry (GC-MS) analysis and antioxidant activity of Curcurbitapepo L. using its leaf sample with standard methods. The sample used for the study was procured from Imo State University school farm and was properly identified. Result of phytochemical screening revealed the presence of saponins, flavonoids, alkaloids, steroids, phlobactannins, proteins, and anthraquinnones, while the GC-MS analysis revealed a total of 78 compounds, out which Bis(2-ethylhexyl) phthalate (C24H38O4) had the highest molecular weight, 2,4,6-Octatriene, 2,6-dimethyl- (C10H16) had the highest peak area of 10.21% while Morphinan-6-ol, 4,5-epoxy-N-methyl-, (5α 6α- (C17H21NO2) had the highest retention time. The antioxidant activity of the studied sample was enhanced against the control. Some of the compounds as revealed by GC-MS analysis could be of healthcare or industrial importance. There is need for further studies on the leaf sample to ascertain further the observations of the present study. This study has evaluated the phytochemical screening, GC-MS analysis and antioxidant activity of C.pepo L. using its leaf sample.

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