Ferropsis cell death can cause complications that may be difficult to detect and quantify: autophagy role and possible therapeutics
It is possible to understand both the processes of ferroptosis and how this type of cell death will be harnessed in the near future. The novel ferroposis-based therapies will also be created and evaluated using various biomarkers. Selenium and vitamin E have been shown to support some types of cancer in several studies. Such environmental factors should be accounted for while exploring ferroaptosis's duties. The effect of these modifications will be dictated by the cellular and environmental context in which they are created. It is critical to determine whether the inhibitor can act as a lipophilic radical-trapping antioxidant and prevent ferroPTosis independently of the enzyme under investigation, as several studies with lipoxygenases have shown.Using pharmacological approaches to evaluate ferropsosis can have potential side effects that are difficult to measure. Using small-molecule probes may be investigated using small-molecules probes. It's essential to utilize a biochemical test or a pharmacodynamic marker of target inhibition when employing RSL3 to inhibit GPX4, or when using erastin to inhibit the system xc–cystine/glutamate antiporter, or by using an Xc–Cystine/Glutamate/Glutamic antiporter inhibitor, or an erast inhibitor, to inhibit Gpx4, GSH, and other types of protein thiols, such as GSH and protein thiola. Many tiny molecules that are potent and selective probes in cellular assays have limited applicability in animal studies. RSL3, a poor solubility, is useful only in instances when an injection is made directly into tissues or tumors. Interfering with iron to modulate ferroptosis has far-reaching ramifications. Findings obtained with iron chelators only should be interpreted with caution as depleting iron can have other side effects other than ferroPTosis suppression.