scholarly journals Semen and haematological responses of rabbit bucks administered oral folic acid

2021 ◽  
Vol 6 (1) ◽  
pp. 1-7
Author(s):  
Wisdom Amaduruonye ◽  
◽  
Joshua Nathaniel ◽  
Christopher Agboje Agida ◽  
Yakubu Ibrahim ◽  
...  
Keyword(s):  
Folic Acid ◽  
Oral Administration ◽  
White Blood Cells ◽  
Sperm Concentration ◽  
Zealand White Rabbit ◽  
Haematological Parameters ◽  
Randomized Design ◽  
Semen Characteristics ◽  
Blood Formation ◽  
The Impact

Some researchers have inferred that folic acid is necessary for reproduction and could enhance blood formation. Thus, a Completely Randomized Design Experiment (CRD) was conducted to evaluate the impact of oral administration of folic acid on the semen and haematological characteristics of New Zealand White rabbit bucks. The treatments designated treatment 1 (T1), treatment 2 (T2) and treatment 3 (T3) having 12 rabbits each were replicated 3 times with 4 rabbits per replicate. The ages of the 36 pre-pubertal rabbit bucks were between 2 to 3 months, and weighed approximately 2.56 kg. Three experimental diets were formulated to meet the nutrient requirements of rabbit bucks. Each rabbit buck on T1 were orally administered folic acid at 0.0 mg, T2 2.5mg folic acid and T3 5.0 mg folic, respectively. Data were collected for semen characteristics and haematology from the rabbit bucks. Data collected on different parameters were subjected to analysis of variance (ANOVA). Results showed that significant increases (p<0.05) were observed on libido, semen pH, spermatozoa progressive motility (67.40-80.20%), spermatozoa live proportion (83.01-94.12%), sperm concentration (112.24-133.80 x106/ml), total number of sperm per ejaculate (50.65-67.66 x106/ml), total viable sperm (291.58-496.69 x109/ml), normal sperm proportion (85.16-91.64%). Also, significant reductions (p<0.05) were observed on the percentage head abnormality of the spermatozoa (3.74-3.18), total abnormality (2.13-0.93), mid-piece abnormality (2.35-0.79), cytoplasmic abnormality (7.17-2.89), and total abnormality (14.84-8.35); while the haematological parameters such as haemoglobin (13.53-14.20g/dl), packed cell volume (33.00-34.96%), white blood cell (6.81-7.80 x103mm3) and the differential white blood cells improved significantly (p<0.05) following the oral administration of folic acids to the rabbit bucks. Thus, the oral administrations of folic acid at 5.0 mg per rabbit buck most significantly improved the semen characteristics, enhanced the overall spermatozoa morphology, reduced sperm cells abnormalities and also improved some haematological parameters of the rabbit bucks.

scholarly journals Haematological reference intervals of sows at end gestation in ten French herds, the impact of parity on haematological parameters and the consequences on reproductive performance

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Gwenaël Boulbria ◽  
Charlotte Teixeira Costa ◽  
Valérie Normand ◽  
Véronique Bachy ◽  
Daphné Rochel ◽  
...  
Keyword(s):  
Reproductive Performance ◽  
White Blood Cells ◽  
Clinical Pathology ◽  
Reference Intervals ◽  
Production Performance ◽  
Haematological Parameters ◽  
Haematological Parameter ◽  
Haematological Values ◽  
American Society ◽  
The Impact

Abstract Background Changes in haematological values occur during the reproductive cycle. In veterinary swine practice, haematological reference intervals for this period are scarce. Over past decades, there has been a remarkable increase in reproductive prolificacy, possibly making previously established haematological reference intervals for sows outdated. Objectives The aim of this study was to provide updated haematological reference intervals for sows at end-gestation, to study the influence of parity on those haematological parameters and to evaluate the impact of haemoglobin levels on production performance. Methods The data presented in this article were obtained using blood samples from 198 apparently healthy and conventionally managed group-housed sows at end-gestation from ten breeding herds located in France. The samples were analysed for haematological variables using impedance technique on Horiba ABX analyser (Horiba, Kyoto, Japan). The reference intervals were calculated according to the guidelines of The American Society for Veterinary Clinical Pathology using SUMMARY procedure in R Studio. Analysis of variance (ANOVA) models were used to evaluate the influence of parity on each haematological parameter and the impact of haemoglobin values on production performances at farrowing. Differences were considered as significant if p < 0.05. Results Reference intervals produced in this study were similar to previously published references but we noticed marked differences in white blood cell values. The study of the impact of parity revealed significant changes for gilts and parity 5 + sows regarding haematological values. Gilts had higher red and white blood cells counts, haemoglobin values and haematocrit values. Regarding haemoglobin values, the higher the number of liveborn and weaned piglets per litter, the lower the haemoglobin value at end-gestation. For sows of fifth or higher gestation, we found that the higher the percentage of stillborn piglets, the lower the haemoglobin value at end-gestation. Conclusions This study provides haematological reference intervals for sows at end-gestation. These will be useful for swine veterinarians and researchers for a better understanding of the influence of parity on haematological parameters and haemoglobin values and their relation to reproductive performance.

Influence of DAFS-25+polizon composition on blood and functional state of liver of steers at fattening

2020 ◽  
pp. 15-18
Author(s):  
Inna R. Kilmetova ◽  
◽  
Igor A. Rodin ◽  
Nazira I. Khayrullina ◽  
Nikolay G. Fenchenko ◽  
...  
Keyword(s):  
Functional State ◽  
White Blood Cells ◽  
Live Weight ◽  
The Body ◽  
Farm Animals ◽  
Control Group ◽  
Standard Diet ◽  
The Impact ◽  
Experimental Group ◽  
Stimulation Of

Summary. The disbalanced feeding and the uneven distribution of micro- and macroelements in the environment leads to a trace element, in particular hypomelanosis. To accelerate the growth and preservation of young farm animals include in the diet of various biological additives and drugs, which include selenium. For stimulation of weight gain in the livestock industry, as well as for the prevention and treatment of pathological processes in addition to micro - and macrouse amino acids, primarily methionine. The aim of this work was to study the influence of composition of DAFS-25+Polizon on morpho-biochemical parameters of blood and functional state of the liver in fattening bulls of black-motley breed in the conditions of the Republic of Bashkortostan. Experiments using were conducted on bull-calves of black-motley breed of the properties in the properties age from 6 to 15 months. The first experimental group during the experiment was additionally given the composition of DAFS-25+Polizon at a dose of 2 mg/kg, the animals of the control group received a standard diet. To assess the impact of the composition DAFS-25+Polizon on metabolism cattle studied morphological and biochemical indicators of blood and conducted histological examination of the liver. It is established that the use of the composition of DAFS-25+Polizon at a dose of 2 mg/kg increases the number of erythrocytes and hemoglobin in the experimental group and reduces the amount of white blood cells. The serum content of total protein, phosphorus and calcium increases in the group of experimental animals. Microscopic examination of the liver revealed no changes in the structure of the organ and hepatocytes in the experimental group, whereas in the control group hemodynamic disorders and dystrophic changes in liver cells were observed. Thus, the use of the composition DAFS-25+Polizon at a dose of 2 mg/kg of live weight in fattening bulls black-and-white breed contributes to the increase of redox processes in the body, stimulation of metabolism, prevent the development of liver disorders of cellular mechanisms of metabolism, optimizes the structure of the liver, which generally provides higher productivity.

Innovative approaches to the correction of chronotrope status of pregnant and lactating women

2016 ◽  
pp. 37-40
Author(s):  
S.I. Zhuk ◽  
◽  
K.K. Bondarenko ◽  
Keyword(s):  
Folic Acid ◽  
Neural Tube ◽  
Active Form ◽  
Biologically Active ◽  
Lactating Women ◽  
Pathological Conditions ◽  
Prevention And Treatment ◽  
The Impact ◽  
High Bioavailability ◽  
Key Words Mthfr

Most recent studies show the impact of violations in the metabolism of folate and metin period in the pathogenesis of neural tube defects (NTD) of the fetus. Metafolin has a number of advantages, which primarily includes direct intake of substances in biologically active form and the optimum effect, even in the case when the patient homozygote and/or heterozygote genotype 677С T polymorphism in MTHFR. With the aim of prevention and treatment of various pathological conditions related to folate deficiency during pregnancy, it is advisable to apply vitamin-mineral complexes, containing metafolin - active form of folate with high bioavailability. Key words: MTHFR, metafolin, folic acid, pregnancy.

Impact of Pseudomonas putida on the Growth Parameters of Fenugreek (Trigonella foenum- graecum L.): A Traditional Herbal Boon for Rural Areas

2017 ◽  
Vol 5 (2) ◽  
pp. 93-96
Author(s):  
Shashi Kant Shukla ◽  
◽  
Awadhesh Kumar ◽  
Anupam Dikshit ◽  
◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Rural Areas ◽  
Growth Parameters ◽  
Leguminous Plant ◽  
Food Protein ◽  
Botany Department ◽  
Medicinal Value ◽  
Randomized Design ◽  
Trigonella Foenum Graecum L ◽  
The Impact

The present study aims the impact of Pseudomonas putida on different growth parameters of Trigonella sp., a leguminous plant to support the requirement of food, protein along with their medicinal value in the rural areas of India. A pot experiment was arranged based on completely randomized design with four replications at Biological Product Laboratory, Botany Department, University of Allahabad. Treatments were given at the seed level with one of control. Results indicated that application of P. putida significantly improved vegetative growth and showed an edge on the growth of the fenugreek as compared to the control.

scholarly journals Comparison of Selected Immune and Hematological Parameters and Their Impact on Survival in Patients with HPV-Related and HPV-Unrelated Oropharyngeal Cancer

Cancers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 3256
Author(s):  
Adam Brewczyński ◽  
Beata Jabłońska ◽  
Agnieszka Maria Mazurek ◽  
Jolanta Mrochem-Kwarciak ◽  
Sławomir Mrowiec ◽  
...  
Keyword(s):  
Prognostic Factors ◽  
Oropharyngeal Cancer ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Post Treatment ◽  
Neutrophil Lymphocyte Ratio ◽  
Lymphocyte Ratio ◽  
Hpv Status ◽  
Pre Treatment ◽  
The Impact

Several immune and hematological parameters are associated with survival in patients with oropharyngeal cancer (OPC). The aim of the study was to analyze selected immune and hematological parameters of patients with HPV-related (HPV+) and HPV-unrelated (HPV-) OPC, before and after radiotherapy/chemoradiotherapy (RT/CRT) and to assess the impact of these parameters on survival. One hundred twenty seven patients with HPV+ and HPV− OPC, treated with RT alone or concurrent chemoradiotherapy (CRT), were included. Patients were divided according to HPV status. Confirmation of HPV etiology was obtained from FFPE (Formalin-Fixed, Paraffin-Embedded) tissue samples and/or extracellular circulating HPV DNA was determined. The pre-treatment and post-treatment laboratory blood parameters were compared in both groups. The neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte ratio (PLR), monocyte/lymphocyte ratio (MLR), and systemic immune inflammation (SII) index were calculated. The impact of these parameters on overall (OS) and disease-free (DFS) survival was analyzed. In HPV+ patients, a high pre-treatment white blood cells (WBC) count (>8.33 /mm3), NLR (>2.13), SII (>448.60) significantly correlated with reduced OS, whereas high NLR (>2.29), SII (>462.58) significantly correlated with reduced DFS. A higher pre-treatment NLR and SII were significant poor prognostic factors for both OS and DFS in the HPV+ group. These associations were not apparent in HPV− patients. There are different pre-treatment and post-treatment immune and hematological prognostic factors for OS and DFS in HPV+ and HPV− patients. The immune ratios could be considered valuable biomarkers for risk stratification and differentiation for HPV− and HPV+ OPC patients.

scholarly journals Effect of Oral Administration of Selenium and Vitamin E on the Quality of Fresh, Refrigerated and Frozen Semen in French Bulldog Breed Dogs

2017 ◽  
Vol 45 (1) ◽  
pp. 7
Author(s):  
Marcelo George Mungai Chacur ◽  
Mariana Grandis Ripari de Souza ◽  
Camila Dutra de Souza ◽  
Camila Pires Cremasco
Keyword(s):  
Vitamin E ◽  
Citric Acid ◽  
Oral Administration ◽  
Liquid Nitrogen ◽  
Sperm Concentration ◽  
Oral Supplementation ◽  
Frozen Semen ◽  
French Bulldog ◽  
Fresh Semen

Background: New methodologies have been developed seeking to maximize pregnancy rate in female dogs created in commercial kennels, and also in order to maintain the quality of canine semen after dilution, refrigeration or freezing. One of the main factors that generate damage to sperm is oxidative stress, to minimize sperm damage, selenium and antioxidants like vitamin E are administered, by oral administration, seeking to improve the quality of semen. The objective was to study the effect of vitamin E and selenium, by oral administration, in the quality of fresh, refrigerated and frozen semen in adult dogs French Bulldog breed.Materials, Methods & Results: Semen samples were collected from 5 adult dogs, French Bulldog breed, being 2 semen drawing before the daily oral supplementation with vitamin E and selenium (ESE®) and semen drawing at 20, 40 and 60 days after the beginning of oral supplement. The ejaculated samples were diluted in TRIS - fructose citric acid (3.28 g TRIS-hydroxy-methyl-amino-methane, 1.78 g of citric acid monohydrate and 1.25 g of D - fructose, dissolved in 100 mL of distilled water and added of 20% egg yolk and 6% of glycerol. The characteristics evaluated in fresh semen were: volume (mL), color, appearance, concentration (x106 / mL), sperm motility (%), sperm strength (1 to 5) and morphology (%). For refrigerated and frozen semen were analyzed: sperm motility (%), sperm strength (1-5) and morphology (%). Diluted semen samples were centrifuged at: 1500 g/10 min and “pellets” formed by sperm of each ejaculated, detached from the tube wall were diluted homogeneously in the diluent TRIS type up to the final volume of 1.5 mL. After that, packaged in 0.5 mL French straws, kept under refrigeration at 5ºC/4 h, placed in nitrogen vapor at -120ºC/15 min, and dipped in liquid nitrogen at -196ºC and then stored on identified rachis and stored in liquid nitrogen container until the time of thawing in  water bath at 37°C/30 s for semen microscopic analysis. Data from fresh, refrigerated and frozen semen were statistically analyzed by analysis of variance and the average compared by 5% of Tukey test. Fresh semen sperm concentration differed (P < 0.05) between the samples, rising after 40 days after the beginning of oral supplementation with selenium and vitamin E. For the spermatic strength, better score (P < 0.05) was observed at collection 4, in 40 days after the beginning of oral supplementation to dogs. For fresh and refrigerated semen, the total defects, defects of head, acrosome and tail did not differ (P > 0.05) between the samples. Total sperm defects and minor head and tail defects did not differ (P > 0.05) between the samples in post-thawing. Regarding the acrosome defects after thawing, there was a significant reduction (P < 0.05) in samples performed 40 and 60 days after the beginning of oral supplementation with selenium and vitamin E.Discussion: Attention should be paid for what purpose the extenders within the refrigeration or freezing biotech will be used. The managed supplement, by oral administration, containing selenium and vitamin E, influenced beneficially raising the sperm concentration in fresh semen and decreasing the acrosome defects in frozen semen. Oral administration of supplementation with selenium and vitamin E is recommended for improving the quality of fresh and frozen semen in dogs.

The impact of fibropapillomatosis on clinical characteristics, blood gas, plasma biochemistry, and hematological profiles in juvenile green turtles ( Chelonia mydas)

2020 ◽  
Vol 96 (4) ◽  
pp. 723-734
Author(s):  
Tsung-Hsien Li ◽  
Chao-Chin Chang
Keyword(s):  
White Blood Cells ◽  
Extracellular Fluid ◽  
Chelonia Mydas ◽  
Sea Turtle ◽  
Total Carbon ◽  
Blood Gas ◽  
Green Turtles ◽  
Cell Counts ◽  
Significant Difference ◽  
The Impact

Fibropapillomatosis (FP) is a tumor- forming disease that afflicts all marine turtles and is the most common in green turtles (Chelonia mydas). In this study, the morphometric characteristics, blood gas, biochemistry, and hematological profiles of 28 (6 FP-positive and 22 FP-negative) green turtles from the coast of Taiwan were investigated. The results indicated that body weight ( P < 0.001) and curved carapace length (CCL; P < 0.001) in green turtles with FP were significantly higher than in turtles without FP. Furthermore, green turtles with FP had a significantly lower value of hemoglobin (HB; P = 0.010) and packed cell volume (PCV; P = 0.005) than turtles without FP. Blood cell counts of white blood cells (WBC; P = 0.008) and lymphocytes ( P = 0.022) were observed with significant difference; green turtles with FP had lower counts than turtles without FP. In addition, turtles with FP had significantly higher pH ( P = 0.036), base excess in extracellular fluid (BEecf; P = 0.012), bicarbonate (HCO3– ; P = 0.008), and total carbon dioxide (TCO2 ; P = 0.025) values than turtles without FP. The findings of this study provide valuable clinical parameters for the medical care of the species in sea turtle rehabilitation centers and help us to understand the physiological response of green turtles to different tumor-forming conditions.

scholarly journals Intermittent Iron Folate Supplementation: Impact on Hematinic Status and Growth of School Girls

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Aditi Sen ◽  
Shubhada Kanani
Keyword(s):  
Body Mass Index ◽  
Folic Acid ◽  
Weekly Dose ◽  
Folate Supplementation ◽  
Standard Methods ◽  
Pubertal Growth ◽  
School Girls ◽  
Iron Folic Acid ◽  
One Year ◽  
The Impact

In view of high iron needs for adolescent growth, this paper studied the impact of daily vs. intermittent (once and twice weekly) iron folic acid (IFA) supplementation on hemoglobin levels and pubertal growth among primary school girls in early adolescence (9–13 years) of Vadodara, India. Methods. Hemoglobin (Hb), height and weight of the girls were assessed using standard methods. In three experimental schools (ES) IFA tablets in a dose of 100 mg Fe+0.5 mg folic acid was given either daily, once weekly or twice weekly for one year. The fourth school (control: CS) did not receive any intervention. Results. Hb levels significantly improved (P<0.01) in all ES compared to CS. Body Mass Index (BMI) increment in ES vs CS was significant (P<0.05) in twice weekly IFA and daily IFA. Within ES groups, mean Hb and BMI increments were comparable between twice weekly IFA and daily IFA. Anemic ES girls showed higher Hb and BMI increments vs. non-anemic girls. Better the Hb response, greater was the benefit on BMI. Conclusion: Twice-weekly IFA supplementation was comparable to daily IFA as regards impact on Hb and growth; at less cost and greater feasibility. Once-weekly dose was inadequate to significantly improve growth.

scholarly journals 158 Provision of artificial shade before weaning influences performance of Angus and Brangus cow-calf pairs

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 129-130
Author(s):  
Gleise Medeiros da Silva ◽  
Tessa M Schulmeister ◽  
Federico Podversich ◽  
Federico Tarnonsky ◽  
Maria E Zamora ◽  
...  
Keyword(s):  
Body Condition Score ◽  
Average Daily Gain ◽  
Beef Cows ◽  
Paspalum Notatum ◽  
Gestation Length ◽  
Long Term Effects ◽  
Weaning Weight ◽  
Randomized Design ◽  
Condition Score ◽  
The Impact

Abstract A completely randomized design study with a 2 × 2 factorial arrangement of treatments evaluated the impact of artificial shade (SHADE or NO SHADE) and breed (ANGUS vs. BRANGUS) on performance of cows, nursing calves, and subsequent offspring. Twenty-four Angus and 24 Brangus black-hided pregnant cows (579 ± 8 kg BW; 6.5 yr; approximately 85 d of gestation) and their nursing calves were randomly allocated to 12 ‘Pensacola’ bahiagrass pastures (Paspalum notatum Flüggé; 1.3 ha; n = 4 pairs/pasture), with or without access to artificial shade (NO SHADE BRANGUS [NSB], NO SHADE ANGUS [NSA], SHADE BRANGUS [SB], and SHADE ANGUS [SA]) for 56 d during summer. Body condition score (BCS) of cows and BW of pairs were obtained on d -1, 0, 55, and 56 (weaning weight). Following weaning, calves were randomly allocated to 4 pens (n = 12/pen) equipped with GrowSafe feed bunks for 14 d to measure feed intake (DMI) and efficiency (G:F). A shade × breed interaction (P &lt; 0.05) was observed for average daily gain (ADG) and BCS of cows, with SB being greatest (P ≤ 0.05). Pre-weaning calf ADG tended to be greater (P = 0.10) for SHADE vs. NO SHADE. Weaning weight and BW 14-d post-weaning were lesser for NSB vs. NSA, SA, and SB, whereas no differences in DMI, ADG, or G:F were observed (P &gt; 0.11). Gestation length was greater for SHADE vs. NO SHADE cows (292 vs. 274; P = 0.02), but calf birth weight was not different. Providing artificial shade to pregnant-lactating beef cows positively impacted the growth of Brangus but not Angus cows. However, weaning BW of calves from Angus cows regardless of shade access did not differ from that of Brangus calves with shade. Further research should investigate the potential long-term effects of shade on the subsequent offspring.

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