scholarly journals ISOLASI DAN IDENTIFIKASI SENYAWA METABOLIT SEKUNDER EKSTRAK METANOL DAUN KELOR (Moringa oleifera Lamk.)

2017 ◽  
Vol 6 (2) ◽  
Author(s):  
Yuszda K. Salimi ◽  
Nurhayati Bialangi ◽  
Saiman Saiman
Keyword(s):  
Secondary Metabolite ◽  
Moringa Oleifera ◽  
Methanol Extract ◽  
Group Identification ◽  
Electron Transition ◽  
Flavonoid Compound ◽  
Isolation And Identification ◽  
Ir Spectrophotometry ◽  
Function Group ◽  
Layer Chromatography

A study has been conducted on the isolation and identification of secondary metabolite compounds from methorol extract of kelor leaf (Moringa oleifera Lamk.). This study aims to isolate and identify secondary metabolite compounds from leaf kelur methanol extract. Moringa leaf was macerated with methanol and obtained a yield of 16.7% methanol extract. The extract of methanol separated by column chromatography yielded 272 fractions. The fraction was purified and analyzed by thin layer chromatography using eluent n-hexane: ethyl acetate (7: 3) and obtained stain spots with Rf (0.61) and (0.47). Phytochemical results of positive isolates on flavonoids test. The results of identification using UV-Vis spectrophotometry and infrared spectrophotometry is a flavonoid compound. The identification using UV-Vis spectrophotometry yields I absorbing bands at 250 nm wavelength. The absorption at 250 nm wavelength is suspected because of the non-bonding electron transition to the σ anti-bonding orbital (n → σ *) by an unconjugated ausochrome suspected to be a hydroxyl functional (OH) group. Identification using infrared (IR) spectrophotometry showed the presence of a bound OH function group, C = O, C = C aromatic, C-H aliphatic, C -O alcohol and = C-H aromatic. Telah dilakukan penelitian tentang isolasi dan identifikasi senyawa metabolit sekunder dari ekstrak metanol daun kelor (Moringa oleifera Lamk.). Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi senyawa metabolit sekunder dari ekstrak metanol daun kelor. Daun kelor dimaserasi dengan metanol dan diperoleh rendemen ekstrak metanol 16,7%. Ekstrak metanol dipisahkan dengan kromatografi kolom menghasilkan 272 fraksi. Fraksi dimurnikan dan dianalisis dengan kromatografi lapis tipis menggunakan eluen n-heksan:etil asetat (7:3) dan diperoleh bercak noda dengan Rf (0,61) dan (0,47). Hasil uji fitokimia isolat positif terhadap uji flavonoid. Hasil identifikasi mengunakan spektrofotometri UV-Vis dan spektrofotometri inframerah merupakan senyawa flavonoid. Identifikasi menggunakan spektrofotometri UV-Vis menghasilkan I pita yang menyerap pada panjang gelombang 250 nm. Serapan pada panjang gelombang 250 nm di duga karena adanya transisi elektron yang tidak berikatan ke orbital σ anti-ikatan (n→σ*) oleh suatu ausokrom yang tidak terkonjugasi yang diduga merupakan gugus fungsional hidroksil (OH). Identifikasi menggunakan spektrofotometri inframerah (IR) menunjukkan adanya gugus fungsi OH terikat, C=O, C=C aromatik, C-H alifatik, C−O alkohol dan =C-H aromatik.

scholarly journals Phytochemical Screening and TLC Profiling of Various Extracts of Reinwardtia indica

2017 ◽  
Vol 9 (4) ◽  
Author(s):  
Mehta Sonam ◽  
Rana Pawan Singh ◽  
Pooja Saklani
Keyword(s):  
Aqueous Extract ◽  
Methanol Extract ◽  
Scientific Basis ◽  
Phytochemical Screening ◽  
Isolation And Identification ◽  
Aerial Parts ◽  
Biochemical Compound ◽  
Ethanol Extracts ◽  
Layer Chromatography ◽  
The Himalaya

Reinwardtia indica, belongs to family Linaceae known as yellow flax or pyoli commonly found in the Himalaya. The plant has varied ethno medicinal importance such as aerial parts are used to prevent bleeding of cuts and as mouthwash; leaves are used in the treatment of paralysis and as natural antibiotic. Qualitative phytochemical screening of chloroform, acetone, ethanol, methanol and aqueous extracts was performed to explore scientific basis of ethno medicinal potential. It confirmed the presence of many phytochemicals like alkaloids, flavanoids, phenols, tannins, saponins, terpenoids, phlobatanins etc. in various extracts. Most of the phytochemicals were found in methanol and ethanol extracts. Thin Layer chromatography (TLC) of the acetone, methanol, chloroform and aqueous extract was performed for four important phytochemicals alkaloids, flavanoids, tannins and phenol. Flavanoids showed their presence in all extracts with one spot in each (Rf 0.8 for acetone, 0.918 for methanol, 0.816 for chloroform and 0.737 for aqueous extract). Alkaloids and tannins were found in acetone and methanol extract while phenol was present only in methanol extract (Rf 0.8). These findings provided the evidence that Reinwardtia indica is a potent source for some medicinally important phytochemicals and it justifies its use as a medicinal plant. This can be further investigated for the isolation and identification of active biochemical compound of medicinal utilities

scholarly journals Isolation of Secondary Metabolite A. niger “In-Habiting” Queen M. gilvus Hagen.’s Nest

2018 ◽  
Vol 5 (2) ◽  
pp. 61
Author(s):  
Yohannes Alen ◽  
Atika Melati ◽  
Gemmy Sarina ◽  
Akmal Djamaan
Keyword(s):  
Melting Point ◽  
Ethyl Acetate ◽  
Secondary Metabolite ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Sample Weight ◽  
Chromatography Method ◽  
White Needle ◽  
Layer Chromatography

Aspergillus niger is pathogen fungi that can live in various locations and can live contiguous with many hosts, one of them is queen termite’s nest. The aims of the study were to isolated the secondary metabilite of A.niger. Extraction proccess of secondary metabolite compounds was carried out by maceration method using methanol solvent. Based on that proccess, methanol extract was be yield 4,32% sample weight. Fractination proccess was carried out in the separating funnel using ethyl acetate solvent, which ethyl acetate fraction was be yield 14.39% methanol extract. Separation of the compounds was carried out by column chromatography method using n-hexane and ethyl asetate eluents. Purification of the compounds were done by recrystallization method using n-hexane and ethyl asetate. Two secondary metabolite compounds were successfully isolated from ethyl acetate fraction of the methanolic extract of fungus A. niger “In-Habiting” queen termite’s nest M. gilvus Hagen. Based on organoleptic examination, the compound signed AM-12-22-01 is 35 mg, white needle crystals, melting point 151-153 oC. While, the AM-12-60-01* is 15 mg, white needle crystals, melting point 91-93 oC. Based on the chemical analysis, thin layer chromatography, ultraviolet and infrared spectra data it was identified that AM-12-22-01 and AM-12-60-01 were a phenolic compounds.Key words: isolation, A. niger, In-Habiting, M. gilvus Hagen.

scholarly journals Test on the Antioxidant Activities of Methanol Extract of Bidara Leaves (Ziziphus spina-christi L.) using the DPPH Radical Immersion Method

2020 ◽  
Vol 3 (1) ◽  
pp. 44-51
Author(s):  
Dwi Bagus Pambudi ◽  
Nuniek Nizmah Fajriyah ◽  
Vidiah Rizka Shalekhah
Keyword(s):  
Free Radicals ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Chemical Compounds ◽  
Bioactive Compound ◽  
Flavonoid Compound ◽  
Immersion Method ◽  
Compound A ◽  
Ic50 Value ◽  
Layer Chromatography

Bidara (Ziziphus spina-christi L.) is a tropical tree originating from Sudan in which it is commonly known as "Nebeq" in Saudi Arabia. It is of a bioactive compound - a flavonoid compound, which is the potential to be used as an antioxidant. It is capable of inhibiting any cell damages caused by free radicals. This study aimed to measure the activities of the free radicals in methanol extracts of Z. spina-christi leaves. The process of extracting the Z. spina-christi leaves was carried out through the maceration method using methanol as a solvent. The qualitative analysis of chemical compounds with certain eluents using thin-layer chromatography (TLC) was purposely to determine the groups of active compounds in extracts. The measurement of antioxidant activities was carried out using the 1,1-Diphenyl-2-picryl Hidrazyl (DPPH) immersion method in which absorption was measured at a maximum wavelength of 513 nm. The results showed that the methanol extract of Z. spina-christi leaves had a very weak antioxidant activity with the IC50 value of 466.804 �g/ml. The results of the bioautographic profile showed the presence of flavonoid compounds, phenols, saponins, and tannins.

scholarly journals ISOLASI DAN IDENTIFIKASI SENYAWA FLAVONOID DARI BATANG CIPLUKAN (Physalis angulata L.)

2019 ◽  
Vol 4 (1) ◽  
pp. 288-296
Author(s):  
Dadan Ridwanuloh ◽  
Fadilah Syarif
Keyword(s):  
Herbal Medicines ◽  
Chemical Compounds ◽  
Flavonoid Compound ◽  
Test Results ◽  
Isolation And Identification ◽  
Chromatography Column ◽  
Physalis Angulata ◽  
Layer Chromatography

ABSTRAK Pemanfaatan tumbuhan di Indonesia banyak digunakan sebagai obat-obatan herbal dan sebagai upaya mempertahankan kesehatan masyarakat. Dari penelitian yang telah dilakukan, baik secara in vitro maupun in vivo, didapatkan informasi bahwa Ciplukan memiliki aktivitas sebagai antihiperglikemi, antibakteri, antivirus, imunostimulan dan imunosupresan (imunomodulator), antiinflamasi, antioksidan, dan sitotoksik. Selain itu dari berbagai laporan tumbuhan ini memiliki banyak khasiat tidak lain karena memiliki kandungan senyawa kimia yang fungsinya dapat mengobati suatu penyakit, salah satu senyawa kimia yang terkandung dalam tanaman Ciplukan ini adalah senyawa flavonoid. Berdasarkan dari hal ini maka dilakukanlah penelitian dengan isolasi dan identifikasi senyawa flavonoid  yang terkandung dalam batang tanaman Ciplukan, Physalis angulata L. Metode yang digunakan adalah Uji Fitokomia, Kromatograpi Lapis Tipis, Kromatografi Kolom, dan Spektrofotometri UV-Vis. Hasil uji fitokimia menunjukan bahwa batang Ciplukan mengandung senyawa Kuinon, flavonoid, saponin, dan monosesquiterpeoid, dan polifenol. Uji KLT dengan eluen n-heksan-etil asetat (7:3) menunjukan kromatogram berwarna biru dengan Rf 0.8 cm. Beberapa fraksi hasil Kromatografi Kolom kemudian di Spektrofotometri UV-Vis menunjukan senyawa tersebut merupakan senyawa flavonoid jenis flavanon. Kata Kunci : Senyawa Flavonoid, Ciplukan (Physalis angulata L.).   ABSTRACT The use of plants in Indonesia is widely used as herbal medicines and as an effort to maintain public health. One type of plant that is used as herbal medicines is the Ciplukan plant. In addition, from various reports of this plant has many other properties because it contains chemical compounds whose function is to treat a disease, one of the chemical compounds contained in this Ciplukan plant is a flavonoid compound. Based on this, a study was conducted with the isolation and identification of flavonoid compounds contained in the stem of the Ciplukan plant, Physalis angulata L. The methods used were Phytochomia Test, Thin Layer Chromatography, Column Chromatography, and UV-Vis Spectrophotometry. Phytochemical test results show that the Ciplukan stem contains Kuinon, flavonoid, saponin, and monosesquiterpeoid compounds, and polyphenols. TLC test with n-hexane-ethyl acetate (7: 3) eluent showed a blue chromatogram with Rf 0.8 cm. Several fractions of the results of the chromatography column later on UV-Vis Spectrophotometry showed that the compound was a flavonoid type of flavanone. Keynote : Flavonoid, Ciplukan (Physalis angulata L.).

scholarly journals IDENTIFIKASI ISOLAT KULIT BATANG WARU (Hibiscus tiliaceus L.) MENGGUNAKAN SPEKTROSKOPI INFRAMERAH

2019 ◽  
Vol 1 (1) ◽  
pp. 14-20
Author(s):  
Muhammad Taupik, M.Sc ◽  
Muhammad Adam Mustapa
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Methanol Extract ◽  
Flavonoid Compound ◽  
Bark Extract ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Layer Chromatography ◽  
Infrared Methods

Waru (Hibiscustiliaceus L.) bark is a plant that is believed and used by community as a traditional medicine in treatment, especiallytotreat fever. This study aims to analyze the level of a secondary metabolite compound in the bark of waru (Hibiscustiliaceus L.) plant calculated using UV-Vis spectrophotometry and infrared methods. The method used to identify the flavonoid content is Thin Layer Chromatography (TLC) using eluent n-hexane and ethyl acetate at the best comparison (7:3). The result obtained from this study is waru bark extract containing flavonoid compound which is shown from the Rf value of the waru bark methanol extract of 0.82, which the value is close to the Rf value of quarcetine 0.83. Analysis of the flavonoid content of waru bark methanol extract has carried out on The Spectrophotometric UV-Vis at a wavelength of 382 nm and the total flavonoid content obtained is of 10 mg of waru bark methanol extract containing135.2166g/mL flavonoid compound with percentage of 13.521% and on the Spetroscopy Infrared, based on the obtained peak shows the existence of functional groups of OH, CH aliphatic, C=C aromatic and C-O indicates that this isolat is a flavonoid compound.

Isolation of the antidiarrhoeal tiliroside and its derivative from Waltheria indica leaf extract

2021 ◽  
Vol 25 (1) ◽  
pp. 86-92
Author(s):  
B.A. Ayinde ◽  
J.O. Owolabi ◽  
I.S. Uti ◽  
P.C. Ogbeta ◽  
M.I. Choudhary
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Methanol Extract ◽  
Preparative Thin Layer Chromatography ◽  
Inhibitory Effects ◽  
Aqueous Fraction ◽  
Vacuum Liquid Chromatography ◽  
Ileum Contraction ◽  
Layer Chromatography ◽  
Dose Dependent

The antidiarrhoeal effect of Waltheria indica methanol extract and fractions have been reported earlier but, the present work examined the intestinal relaxant effects of two flavonoid-phenyl propanoids isolated from the methanol extract. The active aqueous fraction was subjected to vacuum liquid chromatography using dichloromethane with increasing concentration of ethyl acetate, and that of methanol and water successively. The ten (10) fractions obtained were combined to give seven (7). The fraction 2 (C, D) was subjected to preparative thin layer chromatography on silica gel GF254 (10-40μm) using CHCl3-CH3OH (8:2) to obtain compound coded F2. Fraction 4 (F) was subjected to column chromatography using silica gel (60-120μm mesh) and eluted with  dichloromethane with increasing concentrations of methanol. Fractions 9-28 were combined and subjected to column  chromatography using chloroform with increasing concentration of methanol. The fractions 1-16 of these were purified on Sephadex LH-20 to obtain compound BAA. The identities of the two compounds were established using spectroscopic methods. The  antidiarrheal effect of compound F2 was evaluated on mice using charcoal transit (100,200, 400mg/kg), castor oil (40, 60 mg/kg)  while the two compounds were examined for their inhibitory effects on Ach-induced ileum contraction. The effects of the  compounds were compared with loperamide (3mg/kg) and atropine (80μg). Compounds F2 and BAA were identified as tiliroside and 3’’’, 5’’’-dimethoxy tiliroside respectively. Tiliroside inhibited the charcoal transition in the animals in a dose dependent pattern with 400mg/ mL eliciting 63.41% inhibition compared to 59.23% produced by loperamide. The compound also elicited significantly (P<0.05) prolonged onset of stooling and reduced the number and weight of stools produced lower than the control. The two  compounds drastically inhibited the Ach-induced contractions of the ileum. The compound, tiliroside at 10mg, completely abolished  the contraction by Ach unlike 3’’’, 5’’’-dimethoxy tiliroside which reduced the contraction to 1.92% at 20mg. The identified compounds seem to be responsible for the ethnomedicinal use of the plant in treating diarrhea.

scholarly journals ISOLATION, CHARACTERIZATION AND SECONDARY METABOLITE ENDOPHYTIC FUNGAL ISOLATE FROM PERONEMA CANESCENS JACK LEAF AND COPTOSAPELTA TOMENTOSA VAL. K. HEYNE ROOT

2019 ◽  
Vol 4 (5) ◽  
pp. 215-225
Author(s):  
Arsyik Ibrahim ◽  
M. Arifuddin ◽  
Wisnu Cahyo P ◽  
Wahyu Widayat ◽  
Mahfuzun Bone
Keyword(s):  
Secondary Metabolites ◽  
Thin Layer ◽  
Secondary Metabolite ◽  
Fungal Isolate ◽  
Chromatography Method ◽  
Fungal Isolates ◽  
Reaction Test ◽  
Layer Chromatography ◽  
Spray Reagents

Has been done Isolation, Characterization and Secondary Metabolite Endophytic Fungal Isolate from Peronema canescens Jack Leave and Coptosapelta tomentosa Valeton K. Heyne Root. The aim of this research is to know the number of fungal isolates, chromatogram profile and secondary metabolite group of endophytic fungal isolates from P. canencens leaves and C. tomentosa root. Characterization of endophytic fungal isolates was done macroscopically and microscopically. Identification of secondary metabolites endophytic fungal isolates were performed by chemical reaction test and TLC (Thin Layer Chromatography) method with specific spray reagents. The data of this study were obtained based on the number of endophytic fungal that can be isolated, observing macroscopic and microscopic morphological profiles, chromatogram profile and secondary metabolites of each endophytic fungal isolated. The results showed that endophytic fungal that can be isolated from P. canencens leaves four isolates, and two isolates from C. tomentosa root. Morphological profile macroscopic endophytic fungal of the six isolates showed a greenish-colored colony, white gray, clear black. Microscopic profile of each fungal isolate having spores, sprangiosphora, sporangium, conidia, hyphae and stolon. The identified secondary metabolites are: alkaloids, terpenoids, and flavonoids, and polyphenols.

scholarly journals ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF BIXIN PIGMENT FROM ANNATTO (Bixa orellana L.) SEEDS

2010 ◽  
Vol 7 (1) ◽  
pp. 88-92 ◽  
Author(s):  
Pipin T. Kurniawati ◽  
H. Soetjipto ◽  
Leenawati Limantara
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Bixa Orellana ◽  
Isolation And Identification ◽  
Double Beam ◽  
Layer Chromatography

Research on Bixa orellana L. have been done to isolate, identify and determine bixin percentage, the antioxidant and antibacterial activities of bixin from B. orellana seed.  Isolation and identification of bixin was done by thin layer chromatography (TLC), column chromatography, chemical test of bixin and UV-Vis double beam spectroscopy. Percentage of bixin was calculated by JECFA method, the antioxidant activity was determined by DPPH (1-1 diphynilpicrylhidrazil) method while antibacterial activity was analyzed by the use of agar diffusion method. Thin layer chromatography (TLC) for the crude extract contained 5 spot, where spot 5th was bixin. Bixa orellana has 75±3% of bixin. Antioxidant activity of bixin had IC50 548.5±20.0 ppm. Whereas the antibacterial activity of bixin against the Escherichia coli and Staphylococus aureus could be classified as weak inhibition category at 500-750 μg and medium inhibition category at 1500 μg.   Keywords: Bixa orellana L., bixin, antioxidant, antibacteria

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