DNA-fi ngerprinting of local Аbkhazian grape variety Kachich

The study of the indigenous gene pool of Vitis vinifera L. of diff erent zones of viticulture with the involvement of molecular genetic methods is an urgent due to the wide diversity of grape varieties. The territory of Abkhazia is a unique zone, one of the hotbeds of the emergence of cultural grapes. «Kachich» is the oldest Abkhazian grape variety, known for the quality of wines obtained from its harvest. The variety is more resistant to fungal diseases compared to popular European varieties cultivated in modern Abkhazia, and is particularly resistant to rot of berries. However, often the local population calls old bushes by the name «Kachich», which give grapes of good quality for making of red wines and not always these forms correspond to the morphological description of the variety. The aim of the research is DNA fi ngerprinting of the «Kachich» grape variety based on the analysis of polymorphism of microsatellite loci. The object of the study was the plants corresponding to the variety description, growing in the collection of the agricultural company “Wines and waters of Abkhazia” (Sukhum, Abkhazia). DNA was isolated from the young leaves of the top of the shoot of typical plants of the variety by the CTAB-method. Genotyping was carried out by polymerase chain reaction (PCR) with separation of reaction products by capillary electrophoresis. Highly polymorphic SSR-markers recommended for grapes varieties identifi cation were used for study (VVS2, VVMD5, VVMD7, VVMD25, VVMD27, VVMD28, VVMD32, VrZAG62, VrZAG79). The size of amplifi ed fragments was estimated using the automatic genetic analyzer ABI Prism 3130 and special software GeneMapper and PeakScanner. To clarify the size of amplifi ed fragments, the DNA of reference varieties was used. According to the results of microsatellite analysis, the DNA-certifi cate of the genotype of the «Kachich» grape variety was compiled: VVS2153 155 VVMD5234 240 VVMD7239 249 VVMD25239 267 VVMD27186 193 VVMD28234 248 VVMD32262 272 VrZAG62194 196 VrZAG79236 236. The resulting DNA-profi le was checked for matches in the Vitis International Variety Catalogue VIVC. No matches of the obtained DNA-profi le with any other DNA-profi le presented in the Database were found. DNA-certifi cate of genotype «Kachich» can be successfully used for varieties identifi cation of grape plants if necessary to clarify their varietals affi liation.

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Vines for Wines

10.1071/9780643092181 ◽

2004 ◽

Author(s):

George Kerridge ◽

Angela Gackle

Keyword(s):

Point Of View ◽

Cabernet Sauvignon ◽

Wine Grape ◽

Grape Variety ◽

Great Range ◽

Individual Preferences ◽

Colour Photograph ◽

Grape Varieties

Riesling, Chardonnay, Shiraz and Cabernet Sauvignon grapes can make magnificent wines but there are also many other excellent wine varieties that for many of us are rarely experienced. Vines for Wines will expand the wine lover’s knowledge and appreciation of a great range of wines and help to explore their individual preferences for specific varieties, blends, flavours and styles. This book is based on the highly successful Wine Grape Varieties, which is an aid to identifying grape vines. Vines for Wines, however, focuses on wines from the average consumer’s point-of-view, introducing the different wine grape varieties and the wines made from them, including blends. Each variety is represented by a colour photograph of the grape variety, its current world plantings, wine produced and notes describing the varietal characters for each wine grape variety. The tasting terms and wine notes for each variety provide a benchmark for the consumer to assess the quality of wines they drink, and to allow them to share and compare their experiences confidently with other wine lovers.

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Directed formation of commercial quality of table grapes based on the use of foliar dressing with microfertilizers

Magarach. Vinogradstvo i Vinodelie ◽

10.35547/im.2020.22.3.008 ◽

2020 ◽

pp. 225-229

Author(s):

Светлана Валентиновна Левченко ◽

Владимир Александрович Бойко ◽

Дмитрий Юрьевич Белаш ◽

Наталья Васильевна Алейникова

Keyword(s):

Growth Regulator ◽

Grape Variety ◽

Table Grapes ◽

Food And Agriculture ◽

Standard Product ◽

Control Food ◽

The Republic ◽

Product Output ◽

Grape Varieties

На территории Республики Крым проводились двухлетние исследования, которые позволили оценить влияние внекорневых обработок регулятором роста Альбит, ТПС и комплексом удобрений ООО «Биокефарм Рус» («Сиамино Про», Дабл Вин, «Боро Про», Софт Гард, Алга, Мастер Грин Са) на формирование показателей товарного качества столовых сортов винограда в период вегетации виноградного растения. Применение регулятора роста Альбит, ТПС увеличило урожай с куста и среднюю массу грозди винограда сорта Молдова на 26,4 % и 14,8 % относительно контроля (система питания хозяйства). Урожай с куста и средняя масса грозди сорта Италия с использованием препарата Альбит, ТПС повысилась на 34,4 % и 14,9 %, комплекса удобрений ООО «Биокефарм Рус» на 29,5 % и 23,8 % по сравнению с контролем. Комплекс удобрений ООО «Биокефарм Рус» улучшил выход стандартной продукции винограда сорта Италия на 9,4 % в сравнении с контролем. Доля влияния исследуемых препаратов на урожайность сорта Молдова варьировала от 53,1% (Альбит, ТПС) до 90,3% (ООО «Биокефарм Рус») при Р<0.05 (P - значение по критерию Фишера). В опытах с применением регулятора роста «Альбит, ТПС» увеличилась общая дегустационная оценка винограда сорта Молдова - на 14,6 %, сорта Италия - на 5,8 %; при использовании комплекса препаратов ООО «Биокефарм Рус» - на 9,9% (сорт Молдова) и на - 9,6 % (сорт Италия) относительно контроля. On the territory of the Republic of Crimea, a two-year study was carried out, which made it possible to assess the effect of foliar treatments with the growth regulator Albit, RP and a complex of fertilizers of Biokepharm Rus LLC (Siamino Pro, Double Win, Boro Pro, Soft Guard, Algae, Master Green Ca) on the formation of indicators of the commercial quality of table grape varieties during the growing season. The use of growth regulator Albit, RP increased the yield per bush and the average bunch weight of ‘Moldova’ grape variety by 26.4% and 14.8% relative to the control (food and agriculture system). The yield per bush and the average bunch weight of ‘Italia’ variety using Albit, RP preparation increased by 34.4% and 14.9%, using the fertilizer complex of Biokepharm Rus LLC - by 29.5% and 23.8% compared to the control. Fertilizer complex of Biokepharm Rus LLC improved the standard product output of ‘Italia’ grapes by 9.4% compared to the control. The influence degree of preparations studied on the cropping capacity of ‘Moldova’ variety ranged from 53.1% (Albit, RP) to 90.3% (Biokepharm Rus LLC) at P < 0.05 (P is Fisher's variance ratio). In experiments with the use of growth regulator Albit, RP, the overall tasting assessment of ‘Moldova’ grape variety increased by 14.6%, ‘Italia’ variety - by 5.8%; when using the complex of preparations of Biokepharm Rus LLC - by 9.9% (‘Moldova’ variety) and by - 9.6% (‘Italia’ variety) relative to the control.

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Volatile and Non-Volatile Characterization of White and Rosé Wines from Different Spanish Protected Designations of Origin

Beverages ◽

10.3390/beverages7030049 ◽

2021 ◽

Vol 7 (3) ◽

pp. 49

Author(s):

Rubén del Barrio Galán ◽

Héctor del Valle-Herrero ◽

Marta Bueno-Herrera ◽

Pedro López-de la Cuesta ◽

Silvia Pérez-Magariño

Keyword(s):

Geographical Area ◽

Principal Component ◽

Ethyl Esters ◽

Grape Variety ◽

Protected Designation Of Origin ◽

Grape Varieties ◽

Protected Designations Of Origin ◽

The Rose

The quality of wines has often been associated with their geographical area of production, as well as the grape variety used in their elaboration. Many research studies have been carried out to characterize and differentiate between red wines labeled with Protected Designation of Origin (PDO) from different geographical areas, but very few have been carried out on white and rosé wines. The objective of this work was to characterize white and rosé PDO wines from different geographical areas of Spain very close to each other elaborated with different grape varieties and select the variables that most contribute to their differentiation. Analysis of variance (ANOVA) and principal component analysis (PCA) were used as statistical methods. The ethanol content was the nonvolatile variable that most contributed to differentiating between some of the white and rosé wines according to their PDO. The white wines from RD (Ribera del Duero) and BI (Bierzo) were characterized by a high terpenic content (floral notes) while the wines from RU (Rueda), TO (Toro) and CI (Cigales)by a high content of ethyl esters and alcohol acetates (fruity aromas). The rosé wines elaborated with the Mencía grape variety from BI were characterized by their highest polysaccharidic content, which could have a positive sensory effect on the mouthfeel. The rosé wines from CI were characterized by their volatile profile complexity, having the highest content of volatile compounds from the oak wood, terpenes and C6 alcohols which provide pleasant woody, floral and herbaceous aromas. On the contrary, the RD wines were richest in alcohol acetates responsible for fruity aromas.

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Evaluation of the Intrinsic and Perceived Quality of Sangiovese Wines from California and Italy

Foods ◽

10.3390/foods9081088 ◽

2020 ◽

Vol 9 (8) ◽

pp. 1088

Author(s):

Valentina Canuti ◽

Annegret Cantu ◽

Monica Picchi ◽

Larry A. Lerno ◽

Courtney K. Tanabe ◽

...

Keyword(s):

Current Knowledge ◽

Perceived Quality ◽

International Studies ◽

Grape Variety ◽

Protected Designation Of Origin ◽

Wine Experts ◽

Intrinsic Quality ◽

Red Grape ◽

Grape Varieties

Sangiovese is the most cultivated red grape variety in Italy where it is certified for the production of several Protected Designation of Origin (PDO) wines, and it is one of the most cultivated Italian red grape varieties in California. Despite the global distribution of this variety, there is a lack of international studies on Sangiovese grapes and wines. For this reason, the present study aimed to compare 20 commercial Sangiovese wines from 2017 harvest, 9 produced in Italy (Tuscany) and 11 in California, in order to evaluate the intrinsic and perceived quality. The eligibility, identity, and style properties (the intrinsic quality) of the wines were evaluated. A group of 11 Italian experts evaluated the perceived quality by rating the typicality of the wines. The experimental data showed that the intrinsic quality of Sangiovese wine samples was affected by the growing area; in particular, the wine resulted very different for the color indices and polyphenol composition. The above differences in intrinsic quality levels did not lead to a different evaluation of the perceived quality (typicality) by the wine experts. The results evidenced that Sangiovese variety is recognizable also if grown outside its original terroir, and fresh and fruity wines were considered more typical. This study expands our current knowledge of Sangiovese wines and the contribution of regional characteristics to the composition of wine.

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Management of Economics and State "Bottom"

Issues of Risk Analysis ◽

10.32686/1812-5220-2020-17-6-76-91 ◽

2020 ◽

Vol 17 (6) ◽

pp. 76-91

Author(s):

E. D. Solozhentsev

Keyword(s):

Quality Of Life ◽

Artificial Intelligence ◽

Probabilistic Models ◽

Human Life ◽

Special Software ◽

Human Quality ◽

Relationship Of ◽

The Relationship

The scientific problem of economics “Managing the quality of human life” is formulated on the basis of artificial intelligence, algebra of logic and logical-probabilistic calculus. Managing the quality of human life is represented by managing the processes of his treatment, training and decision making. Events in these processes and the corresponding logical variables relate to the behavior of a person, other persons and infrastructure. The processes of the quality of human life are modeled, analyzed and managed with the participation of the person himself. Scenarios and structural, logical and probabilistic models of managing the quality of human life are given. Special software for quality management is described. The relationship of human quality of life and the digital economy is examined. We consider the role of public opinion in the management of the “bottom” based on the synthesis of many studies on the management of the economics and the state. The bottom management is also feedback from the top management.

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The rare, Georgian wine grape in modern enology

ECONOMIA AGRO-ALIMENTARE ◽

10.3280/ecag2019-003013 ◽

2020 ◽

pp. 809-823

Author(s):

Nino Chkhartishvili ◽

Londa Mamasakhlisashvili ◽

Irma Tchanturia ◽

Demetre Bakradze

Keyword(s):

Agricultural Research ◽

Sugar Accumulation ◽

Total Phenol Content ◽

Environment Protection ◽

Grape Variety ◽

Wine Grapes ◽

Scientific Center ◽

Wine Production ◽

Genetic Pool ◽

Grape Varieties

Preserving rare grapes is not just a matter of variety. Recent dna research has shown that unusual and unknown grape varieties provide clues to wine history. During the centuries, 525 Georgian grape varieties had been known but most of them were degenerated. Nowadays, more than 437 rare Georgian vine varieties are preserved in the geo 038 collection of Agricultural Research-Scientific Center, established in 2014 under the Environment Protection and agriculture ministry. Study of the genetic pool of Georgian grape varieties determine the sustainability of the sector and enrichment of the modern wine market. For this reason the studies of the rare aboriginal grape varieties have begun by their ampelography, phenology, chemical, and oeno-caprological characterizes. The aim of this study is to investigate rare, Georgian, aboriginal wine-grapes by their ampelography, chemical and oenolo-caprological characterizes, and to offer different style and aroma wines to the market, enriching the modern wine assortment. The present study investigated firstly the rare Georgian aboriginal grape variety (Chvitiluri) by its characterizes. In this study grapes caprologycal indication, dynamic development of the sugar accumulation, TA and pH in berries, polyphenols extract in skin and seeds, and phenolic compounds were determined. The grape has been compared with Georgian and French wine-grapes and their products as well. The results show that the grape variety - Chvitiluri, and vinification technique (aging on lee) have the significant impact on the total phenol content in wine. White varietal wine contents high alcohol 13,2%, total phenols 733-500mg/l. Wine has specific, varietal characterizes color, taste, aroma. This study indicates that the rare local, aboriginal variety presents the perspective sort for wine production and can be returned in wine production.

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Reproduction of the Roman snail (Helix pomatia L.) from a local natural population in farm conditions and in a natural habitat

Annals of Animal Science ◽

10.2478/aoas-2020-0090 ◽

2020 ◽

Vol 0 (0) ◽

Author(s):

Maciej Ligaszewski ◽

Przemysław Pol

Keyword(s):

Natural Population ◽

Natural Habitat ◽

Local Population ◽

Helix Pomatia ◽

Second Phase ◽

Animal Reproduction ◽

Snail Helix Pomatia ◽

June July ◽

A Site

AbstractThe aim of this study was to compare the quality of clutches and reproduction results of two groups of Roman snails (Helix pomatia) from the same local population, laying eggs simultaneously in semi-natural farm conditions and in a natural habitat. The study material were Roman snails aged 2 or more years which had entered the third phenological season of their life and thus the first season of sexual maturity. Observations were conducted at an earthen enclosure in a greenhouse belonging to the experimental farm for edible snails at the National Research Institute of Animal Reproduction in Balice near Kraków (Poland) as well as at a site where a local population naturally occurs in the uncultivated park surrounding the Radziwiłł Palace. In the June-July season, differences among such parameters as weight of clutch, number of eggs in clutch, mean egg weight, and hatchling percentage when compared to the total number of eggs in the clutch were compared. It was determined that clutches of eggs from the natural population laid in the greenhouse were of lesser weight (P<0.01), contained fewer eggs (P<0.05), and the mean weight of individual eggs was less (P<0.05) than in clutches laid simultaneously in a natural habitat. Both in the greenhouse and the natural habitat, in the first phase of laying eggs (June) the weight of the clutch and number of eggs its contained were greater than in the second phase (July). However, only for snails laying eggs in the greenhouse were these differences statistically significant (P<0.05) and highly significant (P<0.01), respectively. Statistically significant differences were not observed in hatchling percentage between eggs laid in the greenhouse and the natural habitat. The lower number of eggs laid in the farmed conditions of the greenhouse was successfully compensated for by the absence of mass destruction by rodents which occurred in the natural habitat.

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Breeding study of sea buckthorn (Hippophae rhamnoides L.) in the Institute of Horticulture, NAAS оf Ukraine

Horticulture: Interdepartment Subdject Scientific Collection ◽

10.35205/0558-1125-2020-75-37-49 ◽

2020 ◽

pp. 37-49

Author(s):

V.V. Moskalets ◽

◽

T.Z. Moskalets ◽

I.V. Grynyk ◽

O.A. Shevchuk ◽

...

Keyword(s):

Genetic Markers ◽

Cell Biology ◽

Molecular Genetic ◽

The State ◽

Sea Buckthorn ◽

National Academy Of Sciences ◽

Scientific Cooperation ◽

State Register ◽

Academy Of Sciences

The authors present the results of the sea buckthorn breeding at the Institute of Horticulture (NAAS). The stages of the work have been analyzed – from studying and selecting the initial material in the conditions of the Polissya, Polissya-Lisosteppe and Lisosteppe ecotopes (2012-2016) to the successful targeted introduction to the Northern part of the Lisosteppe (2017-2019) and new forms have been characterized according to the traits valuable for economy and molec-ular genetic markers. The new forms of the researched crop taking into consideration the high indices of their productiv-ity,adaptivity to the unfavourable abiotic and biotic environmental factors and consumption quality of fruits for pro-cessing and making functionary products were entered officially into Genetic Fund of the Plants of Ukraine as con-firmed by the certificates of copyright and developed genetic passports. The list of these genotypes includes 1-15-1 (Nos-ivchanka, UA3700073), 1-15-8S (Mitsna, UA3700079), make form 1-15-6Ch (Aboryhen 6/11, UA3700080), 1-15-9 Ka-rotynna, UA3700082), 1-15-3 (Pamiatka, UA3700076), 1-15-8V (Soniachne siayvo, UA3700075), 1-15-11 (Lymonna, UA3700072), 2-15-73 (Morkviana, UA3700077), 1-15-5 (Adaptyvna, UA3700078), 1-15-8B (Osoblyva, UA3700083), 1-15-6 (Apelsynova, UA3700084) and forms 6A/11 (UA3700081), 1-15-5a (Sribnolysta 5a, UA3700074). The possibility of using 5 DNA markers to characterize genotypes of sea buckthorn bymeans of the molecular genetic markers was tested and evaluated in the framework of the scientific cooperation with the Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine. It should be noted that the most polymorphic markers were HrMS025 and HrMS026. However, the marker HrMS014 was monomorphic, but appeared in all the samples, so it can be used as a reference. The best forms of sea buckthorn Adaptyvna (certificate №190899) and Osoblyva (certificate №190900) were included into the State Register of Plant Varieties Suitable for dissemination in Ukraine, and the cultivars of the univer-sal use Nadiina (applications №18299010), Oliana (applications №18299009) and Morkviana (applications № 20299001) and cv pollinator Obrii (applications №18299008) undergo the State strain test. The attention is concentrat-ed on the promising directions of the new sea buckthorn genotypes for the prior breeding and genetic investigations at the Institute of Horticulture (NAAS) and its network.

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Access to health care and Informal Patient Payments for health care in Serbia

European Journal of Public Health ◽

10.1093/eurpub/ckaa166.774 ◽

2020 ◽

Vol 30 (Supplement_5) ◽

Author(s):

S Buch Mejsner ◽

S Lavasani Kjær ◽

L Eklund Karlsson

Keyword(s):

Health Care ◽

Health System ◽

Access To Health Care ◽

Health Care Services ◽

Local Population ◽

Civil Servants ◽

Care Providers ◽

Care Services ◽

Access To Health

Abstract Background Evidence often shows that migrants in the European region have poor access to quality health care. Having a large number of migrants seeking towards Europe, crossing through i.e. Serbia, it is crucial to improve migrants' access to health care and ensure equality in service provision Aim To investigate what are the barriers and facilitators of access to health care in Serbia, perceived by migrants, policy makers, health care providers, civil servants and experts working with migrants. Methods six migrants in an asylum center and eight civil servants in the field of migration were conducted. A complementary questionnaire to key civil servants working with migrants (N = 19) is being distributed to complement the data. The qualitative and quantitative data will be analysed through Grounded Theory and Logistic Regression respectively. Results According to preliminary findings, migrants reported that they were able to access the health care services quite easily. Migrants were mostly fully aware of their rights to access these health care services. However, the interviewed civil servants experienced that, despite the majority of migrants in camps were treated fairly, some migrants were treated inappropriately by health care professionals (being addressed inappropriately, poor or lacking treatment). The civil servants believed that local Serbs, from their own experiences, were treated poorer than migrants (I.e. paying Informal Patient Payments, poor quality of and access to health care services). The interviewed migrants were trusting towards the health system, because they felt protected by the official system that guaranteed them services. The final results will be presented at the conference. Conclusions There was a difference in quality of and access to health care services of local Serbs and migrants in the region. Migrants may be protected by the official health care system and thus have access to and do not pay additional fees for health care services. Key messages Despite comprehensive evidence on Informal Patient Payments (IPP) in Serbia, further research is needed to highlight how health system governance and prevailing policies affect IPP in migrants. There may be clear differences in quality of and access to health care services between the local population and migrants in Serbia.

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Development of new total RNA isolation method for tissues with rich phenolic compounds

Turkish Journal of Biochemistry ◽

10.1515/tjb-2019-0375 ◽

2020 ◽

Vol 45 (4) ◽

pp. 343-350

Author(s):

Zafer Seçgin ◽

Gökhan Gökdemir ◽

Elif Seda Atabay ◽

Aslıhan Kurt Kızıldoğan ◽

Musa Kavas

Keyword(s):

Phenolic Compounds ◽

Rna Sequencing ◽

Rna Isolation ◽

Wall Structure ◽

Isolation Method ◽

High Quality ◽

Ctab Method ◽

Total Rna Isolation

AbstractBackgroundRNAs to be used in transcriptome analysis must be of high quality and pure in order to ensure maximum representation of the expressed genes. RNA isolation is difficult in hazelnut tissues containing large amounts of secondary metabolite, phenolic compounds and the cell wall structure. Commonly used protocols for RNA isolation are those that require a lot of labor and time and also do not allow sufficient RNA isolation when applied to tissues rich in phenolic compounds. This study was aimed to develop an efficient method for isolation of total RNAs from bud of hazelnut to be used in RNA sequencing.Materials and methodsAn optimized new method was successfully applied on three different hazelnuts genotypes (Çakıldak, Palaz, Tombul) and about 25 times higher amount of total RNAs per mg fresh tissues were obtained compared to classical CTAB method. Different methods have been tried for the isolation of RNA from hazelnut tissues and the determination of the quality of the obtained RNAs.ResultsThe quality and quantity of isolalated total RNAs were determined by spectrophotometer, electrophoresis and PCR. This success has been caught without any compromise of purity since A260/A280 ratios ranged from 1.90 to 2.04 and A260/A230 ratios were >2.0 in all purified RNAs.ConclusionThe total RNAs isolated with new protocol was found to be suitable for RNA sequencing and other molecular applications.

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