scholarly journals Chromosomal mutations in high-yield Holstein cows

2021 ◽  
pp. 115-123
Author(s):  
D. V. Samsonov ◽  
S. G. Kulikova ◽  
V. A. Andreeva ◽  
D. A. Aleksandrova
Keyword(s):  
Blood Cells ◽  
Chromosomal Instability ◽  
Western Siberia ◽  
Farm Animals ◽  
Holstein Cows ◽  
High Yield ◽  
Ecological Situation ◽  
Chemical Pollutants ◽  
Diploid Cells ◽  
Chromosomal Mutations

The results of the research of somatic chromosomal instability: aneuploidy and polyploidy in the blood cells of high-yield Holstein cows with the productivity of more than 9000 kg have been presented in the article. The research was conducted in JSC Vaganovo, Promyshlennovsky District, Kemerovo Region on the population of Holstein cows. Sample preparation was carried out accord- ing to P. Moorhead et al. Samples were stained using the Romanowsky-Giemsa method. The authors studied 6068 metaphase plates in a group of 30 healthy animals. Aneuploidy was counted according to the practice of N.P. Bochkov et al. The authors also analysed the ecological situation in the area of keeping and breeding cattle. Studies of soil, fodder, organs and tissues in farm animals of different species confirm that heavy metal and chemical pollutants are not contaminated in Western Siberia, and their levels are within sanitary norms. Therefore, the ecological situation in the study area should be considered safe. The study shows that the frequency of polyploidy was 0.59% in the studied sample. Tetraploidy, triploidy, and hexaploidy rates were 0.33; 0.18 and 0.06, respectively, the frequency of true hypoploidy was 0.73, and the number of diploid cells was 84.7%. The data obtained can be tentatively applied as average values and can also be used to evaluate the interior of the animals.

scholarly journals SOMATIC CHROMOSOMAL INSTABILITY IN HOLSTEIN COWS IN KUZBASS CONDITIONS

2020 ◽  
pp. 123-130
Author(s):  
D. V. Samsonov
Keyword(s):  
Chromosomal Instability ◽  
Western Siberia ◽  
Heavy Metal Contamination ◽  
Normal Values ◽  
Farm Animals ◽  
Holstein Cows ◽  
Holstein Breed ◽  
Structural Aberrations ◽  
Cattle Blood ◽  
Diploid Cells

The results of studies of somatic chromosomal instability aneuploidy and polyploidy, the number of fragments and breaks in the blood cells of Holstein cows with a productivity of over 9000 kg are presented. The studies were carried out at JSC “Vaganovo” of the Promyshlennovskiy district of the Kemerovo Region on a population of Holstein cows. Sample preparation was carried out according to the method of P. Moorhead and co-authors, and their staining according to RomanovskyGiemsa. 2452 metaphase plates of cattle blood were studied. The ecological situation was analyzed in the zone of its keeping and breeding. Studies of soil, feed, organs and tissues in farm animals of various species confirm the fact that there are no heavy metal contamination on the territory of Western Siberia and their level is within the limits of sanitary standards. As a result of the study, the frequency of somatic chromosomal instability, including numerical violations and structural aberrations of chromosomes, was established. In the studied sample, the frequency of polyploidy was 0.74%, tetraploidy, triploidy and hexaploidy 0.41; 0.25 and 0.08%, respectively. The frequency of fragments and breaks was approximately the same - 3.83 and 2.91%, respectively. The overall level of chromosome aberrations was 6.75%. The number of diploid cells was 84.25%. Due to the paucity of data on the somatic chromosomal instability of the Holstein breed in Kuzbass conditions, the data obtained can be preliminarily considered as normal values, and also used in assessing the interior of animals.

Chromosomal instability in different species of agricultural animals (review)

2021 ◽  
pp. 19-26
Author(s):  
V. A. Andreeva
Keyword(s):  
Chromosomal Instability ◽  
Animal Species ◽  
Western Siberia ◽  
Reproductive Function ◽  
Farm Animals ◽  
Chromosomal Disorders ◽  
Important Indicator ◽  
Karyotypic Analysis ◽  
Different Types ◽  
Almost All

The purpose of the work was to analyze the frequency of chromosomal instability in different species of agricultural animals in Western Siberia on the basis of literature data. The analysis of the literature on the topic of somatic chromosomal instability in agricultural animals has been carried out. Despite the stability of the chromosome set, deviations from it are quite common. It is noteworthy that chromosomal instability is characteristic of almost all individuals in the population and serves as an important indicator for assessing the natural mutability of chromosomes. It has been found if an aberration appeared in one tissue, it is very likely to occur in others. It has been noted that somatic chromosomal instability occurs in animals with reduced reproductive function, as well as those suffering from any pathology. For example, in calves with parakeratosis the increase in the frequency of chromatid and isochromatid breaks has been found. Analysis of the frequency of aberrations in sires, which differ in the level of perinatal mortality of offspring revealed the increased percentage of offspring mortality in fathers with the large number of chromosomal disorders. Therefore, low fertilization, spontaneous abortions and stillbirths may be indications for karyotypic analysis. It has been revealed that the lability of the karyotype is inherent in all animal species, regardless of the species, sex and age, as well as the morphofunctional state. The data on some types of somatic chromosomal instability in different types of farm animals in Western Siberia has been presented. Such indicators as the frequency of polyploidy, the number of cells with fragments of chromosomes, as well as single and paired fragments of chromosomes has been given. Different types of chromosomal instability varied depending on the animal species and climate zone. The presented data can be accepted as a physiological norm and used in veterinary medicine and animal science.

scholarly journals THE IMPACT OF THE STUD RAMS’ GENOTYPE ON THE NUMBER OF CHROMOSOMES IN THE OFFSPRINGS’ CELLS

2019 ◽  
pp. 23-31 ◽  
Author(s):  
V. A. Andreeva ◽  
V. Lee ◽  
M. Liu ◽  
R. T. Saurbaeva ◽  
T. V. Konovalova ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Pollution ◽  
Blood Cells ◽  
Chromosomal Instability ◽  
Western Siberia ◽  
Phenotypic Variability ◽  
Breeding Area ◽  
Protection Zones ◽  
Chromosome Fragments ◽  
The Impact

The paper demonstrates the research results on somatic chromosomal instability that includes the number of chromosomal fragments in the blood cells of the ram descendants, obtained from Romanov servicing rams. The research was carried out on the populations of Romanov sheep at OAO “Vaganovo” in Promyshlennovsky district of the Kemerovo region. The samples were prepared by means of the P. Murkhed method and stained using the Romanovsky-Gimza method. 2580 metaphase blood plates of lambs were studied. The authors analyzed the environmental situation in the breeding area. Studies of soil, fodder, organs and tissues of the animals of different species confirm that there are no heavy metal pollution within the sanitary protection zones in Western Siberia. The authors found out the hereditary regularities of somatic chromosomal instability in the Romanov sheep. The researchers identified the impact of servicing rams genotype on the quantity of fragments of chromosomes in the blood cells of the offspring. The differences in phenotypic variability were revealed in the offspring of different ram-males. The paper shows that the number of chromosome fragments in the sons of some fathers was 3.2 times higher than in the descendants of other servicing rams. Due to the lack of the data on somatic chromosomal instability, the number of chromosomal fragments in the cells of Romanov sheep in Siberia, the data obtained can be accepted as a physiological standard, as well as used to characterize animal interior.

CBIO-11. HISTONE H3.3 G34R/V MUTATIONS STIMULATE PEDIATRIC HIGH-GRADE GLIOMA FORMATION THROUGH THE INDUCTION OF CHROMOSOMAL INSTABILITY

2021 ◽  
Vol 23 (Supplement_6) ◽  
pp. vi29-vi29
Author(s):  
Charles Day ◽  
Florina Grigore ◽  
Alyssa Langfald ◽  
Edward Hinchcliffe ◽  
James Robinson
Keyword(s):  
Gene Regulation ◽  
Chromosomal Instability ◽  
High Grade Glioma ◽  
Kinase Assay ◽  
High Grade ◽  
Newborn Mice ◽  
Cycle Arrest ◽  
Epigenetic Gene Regulation ◽  
Diploid Cells

Abstract H3.3 G34R/V mutations are drivers of high-grade pediatric glioma (pHGG). H3.3 G34R/V mutations are linked to altered H3.3 K36 trimethylation (H3K36me3); implicating epigenetic gene regulation as a possible contributor to pHGG formation. Here we show that H3.3 G34R/V also induces chromosomal instability (CIN); a hallmark of pHGG. If CIN promotes pHGG formation is unknown. We observed that H3.3 G34 mutant pHGG cells have reduced mitotic H3.3 S31 phosphorylation compare to WT H3.3 cell lines. And, H3.3 G34R reduced Chk1 phosphorylation at S31 by >90% in an in vitro kinase assay. Chk1 regulates chromosome segregation through phosphorylation of pericentromeric H3.3 S31 during early mitosis. Overexpression of H3.3 G34R or non-phosphorylatable S31A in H3.3 WT, diploid cells caused a significant increase in CIN. Likewise, H3.3 G34 mutant pHGG cells have significantly elevated rates of CIN as compare to H3.3 WT pHGG cells. During normal cell division, phospho-S31 is lost in anaphase. However, following chromosome missegregation, phospho-S31 spreads and stimulates p53-induced cell cycle arrest. Here we show that WT p53 cells expressing mutant G34 fail to arrest following chromosome mis-segregation. These studies demonstrate that H3.3 G34R/V mutations are sufficient to transform normal, diploid cells into proliferating CIN cells. To determine if this process contributes to tumorigenesis, we used RCAS Nestin-TVA mice to overexpress H3.3 WT, G34R, or S31A – P2A-linked to PDGFB expression in glial precursor cells of newborn mice. Over 100 days, S31A and G34R mice had drastically reduced survival (averaging 77, 81, and 100 days for S31A, G34R, and WT). Furthermore, most G34R and S31A mice developed HGG, while H3.3 WT mice remained tumor-free. Our work implicates CIN as a driver of H3.3 G34 mutant pHGG formation. Our ongoing studies utilize K36M and double mutants to further define the contributions of S31 phosphorylation (CIN) and H3K36me3 (epigenetic gene regulation) to tumorigenesis.

The Determination of Nitrate Reductase Activity of Leaves to the Dominant Species in Forest Community of Northern Aspect of Changbai Mountains

2011 ◽  
Vol 183-185 ◽  
pp. 900-904
Author(s):  
Yu Wen Li ◽  
Yun Jie Wu
Keyword(s):  
Nitrate Reductase ◽  
Tree Species ◽  
Reductase Activity ◽  
Forest Community ◽  
High Yield ◽  
Changbai Mountains ◽  
Ecological Situation ◽  
Nr Activity

This paper addresses the application of improvement in vivo of traditional method for determination of nitrate reductase (NR) activity of leaves to dominant tree species in forest community of northern aspect of Changbai Mountains. It describes the NR activity of tree species related to the shade-endurance and shows that the intolerance tree species has higher NR activity. The NR of a species is also related to the ecological situation of the sites. Tree species with higher NR activities should be selected for breeding of fast growing and high yield tree species.

scholarly journals Assessment of the Biological Resource Potential of Species of Symphytum L. in the Flora of the Kabardino-Balkarian Republic

2019 ◽  
Author(s):  
A Ya Tamakhina ◽  
I Sh Dzakhmisheva ◽  
A A Ahkubekova ◽  
Z L Kantsalieva
Keyword(s):  
Nitrogen Availability ◽  
Farm Animals ◽  
Limiting Factors ◽  
High Yield ◽  
Aboveground Phytomass ◽  
Resource Potential ◽  
Average Temperature ◽  
Fodder Production ◽  
Phosphorus And Potassium ◽  
The Relationship

The paper investigates ecological and biological features, chemical composition and nutritional value of the aboveground phytomass of Symphytum asperum Lepech. and Symphytum caucasicum M. Bieb. within the primary range through the example of phytocenoses of the Kabardino-Balkarian Republic. The range of S. asperum covers the foothill and middle mountain zones up to a height of 2400 m a.s.l., and S. caucasicum covers the flat and foothill zones up to 750 m a.s.l. The limiting factors for the species of Symphytum L. are moisture, nitrogen availability and acidity of the soil. In the studied ecotopes, the realized ecological niche of S. asperum and S. caucasicum is within the limits of the fundamental one. The relationship has been established between the content of nitrogen, phosphorus and potassium in the aboveground phytomass of comfrey plants and the content of humus, mobile phosphorus and exchangeable potassium in the soil of the species habitat. The accumulation of vitamin C in the aboveground phytomass depends on the height above sea level, the average temperature and the amount of precipitation during the growing season. The nutritional and energy value of phytomass, high yield and good regrow capacity of plants indicate the possibility of using S. asperum and S. caucasicum in fodder production. Since comfrey plants grow sparsely and in places difficult for farm animals to access, it is advisable to create plantations of S. asperum and S. caucasicum in the territory of Kabardino-Balkaria for multiple purposes (fodder, melliferous and medicinal).

scholarly journals ECOLOGICAL PLASTICITY OF SPRING OATS IN THE CONDITIONS OF WESTERN SIBERIA

2018 ◽  
Vol 48 (3) ◽  
pp. 21-27
Author(s):  
M. A. Kozyrenko ◽  
V. N. Pakul ◽  
D. E. Androsov
Keyword(s):  
Environmental Conditions ◽  
Western Siberia ◽  
Plant Genetic Resources ◽  
Steppe Zone ◽  
Dispersion Analysis ◽  
Yield Stability ◽  
High Yield ◽  
Forest Steppe ◽  
Ecological Plasticity ◽  
The Usa

The works presents the results of studying 165 specimen of spring oats from the world collection The N.I. Vavilov All-Russian Research Institute of Plant Genetic Resources. The research was conducted in the northern forest-steppe zone of Western Siberia in 2014-2016. The soil on which the research was done can be characterized as leached chernozem, heavy loam by texture and particle size distribution, of medium capacity. The content of humus equals 7.8%, reaction of soil solution is close to neutral, рН is 6.0. In the horizon of 0-40 cm the content of N-NO3 is 35.4mg/kg, P2O5 – 122 mg/kg, K2O – 98 mg/kg. An assessment is given to spring oat specimen by the parameters of ecological plasticity bi and yield stability Si2 . The research was conducted in the conditions characterized in accordance with the index of environmental conditions Ij. The most favourable conditions developed in 2016, when the environment index Ij equaled 109.2. Adverse conditions were identifi ed in 2014, when Ij equaled -46.25, and in 2015, when Ij equaled -62.99. By the results of the dispersion analysis the dominating infl uence of environmental conditions on the yield of spring oat specimen is established at 85.3%, the share of genotype infl uence is 2.6%. Eight specimen of spring oats proved to exceed Creole standard variety by yield with the trait variability being from 29.3% to 87.1%. Sources for creation of intensive type varieties, with high responsiveness to favorable conditions for growth and development are identifi ed: IL 86-1158 (to 14316, the USA), Jumbo (to 14702, Germany), Hamilton (to 14761, the USA), Talisman (to 14785, Russia), Omikho (to 14827, Russia), OA 269 (to 14992, Canada), Stayer (to 15181, Russia), Eclipse (to 15187, Russia). High-yielding genotypes which react to environmental conditions to a medium degree (bi is nearing a unit), and have high yield stability (Si2 is nearing zero) are defi ned, namely Express (to 14505, Russia), bi = 1.04, Si2 = 0.053, Yubilyar (to 14723, Belarus), bi = 0.92, Si2 = 0.070, C.I. 7321 (to 14737, the USA), bi = 1.09, Si2 = 0.076, Vallntin (to 15189, Slovakia), bi = 1.13, Si2 = 0.050, CDC Bell (to 14805, Canada), bi = 0.62, Si2 = 0.0065.

scholarly journals Studying the Source Material for Yellow Melilot Selection

2021 ◽  
Vol 285 ◽  
pp. 02019
Author(s):  
Nina Chukhlebova ◽  
Inna Donets ◽  
Anna Golub ◽  
Olga Mukhina
Keyword(s):  
Growth Dynamics ◽  
Animal Husbandry ◽  
Farm Animals ◽  
High Yield ◽  
Quantitative Composition ◽  
Saline Soils ◽  
Summer Drought ◽  
Daily Growth ◽  
Legume Crop ◽  
Digestible Protein

Creation of a system that ensures stable and complete feeding of farm animals is important for an effective development of animal husbandry in Stavropol Territory. Science established and practice confirmed that to organize healthy functioning of animals and their high productivity, it is necessary to have at least 105-110 g of digestible protein per feed unit. However, due to the current use of feed mainly consisting of cereals, there is a deficiency of protein, which is balanced by fodder grains. The lack of protein and its quality in feed has an extremely negative effect on the health of animals, reduces their productivity, impairs reproduction, disrupts metabolism and leads to overspending of feed, and, consequently, to an increase in the cost of livestock products. For the effective use of saline soils, which in the Stavropol Territory account for 24.8% of the total area, it is necessary to select field crops that give high yields of fodder rich in digestible protein, as well as to improve the structure of saline soils by enriching them with ecologically clean nitrogen that does not cause acidification. The legume crop of yellow melilot can become such a crop. Solving the problem of organic farming, it is necessary to expand the assortment of yellow melilot to create a solid fodder base, to use it as the best forecrop and break crop in grain crop rotations. Therefore, the study of some collection material of yellow melilot on leached black soils and the selection of promising samples is relevant for further selection work to create new varieties. A study of the seed material samples of yellow melilot from the VIR collection of different geographical origins was carried out according to the presence of hard seeds; samples with low and high content of them were established. The sowing qualities of yellow melilot collection samples were studied in laboratory conditions. The growth dynamics of the yellow melilot samples was studied, and decades of the maximum daily growth of yellow melilot were established. A high yield of yellow melilot samples in the first and second year of life was shown. The quantitative composition of main nutrients in the fodder mass of the yellow melilot samples of different geographical origins was established. For the conditions of the Stavropol Territory, it is necessary to select samples with economically valuable biological characteristics that can provide high rates of linear growth of green mass before the onset of summer drought.

scholarly journals Possibilities of organic production in Vojvodina

2011 ◽  
Vol 27 (3) ◽  
pp. 1357-1365
Author(s):  
S. Grdovic ◽  
G. Vitorovic ◽  
B. Petrujkic ◽  
B. Mitrovic ◽  
J. Nedeljkovic-Trailovic
Keyword(s):  
Nutritive Value ◽  
Agricultural Land ◽  
Poa Pratensis ◽  
Organic Production ◽  
Farm Animals ◽  
High Yield ◽  
Solid Base ◽  
Organic System ◽  
Nutrition Quality ◽  
Further Development

During the past decade, the general attitude of the human population towards health, nutrition quality and environment has remarkably changed. As a result of the growing demand for organic products, especially organic milk, the livestock organic production has increased. Figures from the Serbian Ministry of agriculture estimate that around 75 percent of Serbia's agricultural land, and some 650,000 hectares, can be used for organic food production within three years. The ?rule of 30%? in organic production means that farm animals kept in organic system must ingest at least 30% of meal dry matter from pastures, makes forage quality of utmost importance. In order to estimate possibilities of organic production in Fruska Gora region forage botanical as well as chemical composition were analysed. A botanical analysis has confirmed presence of plants with high yield and of high nutritive value. Dominant presence of Dactilys glomerata L. and Poa pratensis L. was revealed in the samples of mixed hay, while the Lucerne hay was predominantly composed only by one leguminous species Medicago sativa L. Chemical analyses of hay samples originating from Fruska Gora has pointed out that level of crude proteins, cellulose, fats, macro and trace elements can completely satisfy requirements of animals in organic farming and therefore can present a solid base for further development of organic dairy farming. Possibilities for further development of organic production are great, and it can be expected that organic production will increase in the future and can become a significant branch of livestock production.

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