scholarly journals BACTERIOLOGICAL STUDY OF EAR DISCHARGE IN BIR HOSPITAL

2003 ◽  
Vol 41 (142) ◽  
pp. 318-322 ◽  
Author(s):  
Charu Arjyal ◽  
S Adhikari ◽  
J Shrestha

Discharge from the ear is one of the commonest symptoms of infections of the ear.With a view to study the major strains of bacteria encountered in ear discharges, thisstudy was conducted in Microbiology Laboratory, Bir Hospital. The study wasconducted for a period of 6 months from January 2000 - July 2000. A total of 224samples of aural discharges of the outpatients were collected and analysed for bacterialgrowth. These samples were cultured with the use of standard microbiologicaltechniques and the antibiotic sensitivity pattern of the isolates was also studied invitro with the use of disc diffusion method. Among 224 patients, 50.9% were male and49.1% were female and the age of the patients varied from 6 months to 76 years.Majority of the patients with discharging ear belonged to the age group 11-20 years.Bacterial growth was found in 82.6%. The bacterial isolates were of 13 differentspecies. Staphylococcus aureus was the most frequently isolated organism (49.4%)followed by Pseudomonas aeruginosa(20.9%). Other organisms isolated wereCoagulase negative Staphylococci (CONS), Streptococcus spp., Bacillus sps., Escherichiaspp., Proteus spp., Enterobacter spp., Klebsiella spp., Acinetobacter spp., Edwardsiellaspp.The prevalence of gram positive bacterial isolates was higher than gram negativebacterial isolates. 58.0% of samples showed positive correlation with direct smeargram stain and culture results. Among the antibiotics used, Ciprofloxacin was foundto be the most effective (80.2%) for overall bacterial isolates followed by Gentamicin(68.9%). The result of antibiotic sensitivity was almost similar to gram positive aswell as gram negative isolates.Key Words: Aural discharge, Gram stain, culture, sensitivity.

Author(s):  
Harit Kumar ◽  
Nitin Goel Insan ◽  
Shubham Chauhan ◽  
Varsha A Singh

Objective: To find the antibiotic sensitivity pattern of uropathogens. Methods: A total of 100 urine samples of clinically suspected urinary tract infection were collected from May 2016 to July 2016. The samples were inoculated on cystine lactose electrolyte deficient agar and incubated at 37°C for 24 hrs. Results: A total of 77 (77%) samples were positive showing microbial growth. Among all isolates, Escherichia coli was 36.36%, followed by Klebsiella spp. (35.06%), Citrobacter spp. (6.49%), Staphylococcus aureus (6.49%), Pseudomonas spp. (5.19%), Enterococcus faecalis (3.90%), and Acinetobacter spp. (1.30%). For Gram-positive isolates, the most effective antibiotic was nitrofurantoin followed by sulphafurazole. For Gram-negative isolates, the most effective antibiotic was nitrofurantoin followed by gentamicin and piperacillin/tazobactam. Conclusion: In this study, nitrofurantoin was the most effective antibiotic for Gram-positive and Gram-negative uropathogens


2013 ◽  
Vol 21 (1-2) ◽  
pp. 117-129 ◽  
Author(s):  
MM Rahman ◽  
MN Hossain

Studies were conducted to identify Aeromonas sp. isolates collected from disease affected carp fishes (Cyprinus carpio and Labeo rohita), their antibiotic sensitivity pattern and sensitivity to 121 herb extracts. A total of 19 Aeromonas sp. isolates were identified from a total of 84 different isolates through a series of physiological, morphological and biochemical tests. Seven antibiotics viz., streptomycin, chloramphenicol, erythromycin, sulphamethoxazole, cephradine, oxytetracycline and gentamycin were tested by disc diffusion method where all of the Aeromonas sp. isolates were found sensitive to streptomycin, erythromycin and gentamycin. But, 78.95% of the isolates were found resistant to oxytetracycline. In addition, 5%, 11%, and 16% isolates exhibited resistance to chloramphenicol, cephradine and sulphamethoxazole, respectively. Twenty one percent of the isolates showed multiple resistance to the antibiotics. A total of 121 herb extracts were evaluated for their sensitivity to the fish pathogenic Aeromonas sp. isolates. Among these, 23 herbs were found to possess antimicrobial activity. Leaf extracts of Eugenia caryophyllus, Spondias pinnata and Terminalia chebula were found to inhibit the growth of all the Aeromonas sp. isolates. In this study, multiple antibiotic resistant isolates were also found to be sensitive to several herb extracts. Thus, herbal treatment would promise a greater viable solution for effective treatment of fish disease.DOI:http://dx.doi.org/10.3329/pa.v21i1-2.16758 Progress. Agric. 21(1 & 2): 117 - 129, 2010


2020 ◽  
pp. 1-2
Author(s):  
Spriha Smriti ◽  
Babita Kumari ◽  
Pratulya Nandan

Introduction- Osteomyelitis refers to infection of bone marrow which spread to the bone cortex and periosteum. Osteomyelitis is most commonly caused by pyogenic bacteria. Despite greater advances in treatment the management of osteomyelitis is quite challenging due to increasing development of antibiotic resistance. Material and Methods -100 patients were evaluated. Samples like pus or exudates or pieces of necrotic tissue were taken. Samples were subjected to gram’s staining and culture. Antimicrobial sensitivity was done using Kirby Bauer disc diffusion method. RESULTS - Staphylococcus aureus was the most common organism isolated followed by E.coli, Klebsiella pneumoniae P. aeuroginosa and Proteus. Staphylococcus aureus + E coli was the most common polymicrobial organism isolated. In trauma cases most common organism isolated was Staphylococcus aureus. Gentamycin was the commonest antibiotic which was effective for treatment of osteomyelitis followed by amikacin, tobramycin and levofloxacin.In cases of gram positive organism max were seen to sensitive to vancomycin and linezolid.


2018 ◽  
Vol 25 (12) ◽  
pp. 1949-1953
Author(s):  
Aneela Khawaja ◽  
Faiqa Arshad ◽  
Sadaf Aleem

Introduction: The genetic competencies of bacteria and the resistance have been impeding the usefulness of antibiotic therapy. There has been an alarming increase in the infections caused by Acinetobacter spp. especially the multidrug resistance pattern has narrowed the therapeutic ranges. Objectives: To determine the prevalence and antibiotic sensitivity pattern of Acinetobacter spp., among clinical specimens of tertiary care hospital.Study Design: Descriptive study. Place & Duration of Study: Pathology Department, PGMI, from January 2015 to December 2015. Materials & Methods: Total 8465 clinical specimens were inoculated. Acinetobacter spp. was identified and isolated by the preliminary microbiological and biochemical tests. Antimicrobial susceptibility testing was implemented by modified Kirby-Bauer disk diffusion method as per CLSI guidelines (2015). Results: Acinetobacter spp. isolated in 234 (7.29%) clinical specimens among 3208 (37.89%) culture positive isolates. Out of total 234 Acinetobacter spp. isolates 144 (61.54%) were recovered from male patients and 90 (38.46%) from female patients. the frequency of Acinetobacter spp. isolates was seen highest in CSF (23.07%) and lowest in HVS (5.52%) specimens. Maximum samples were recovered from surgical wards 85 (36.32%), while from pediatrics department only 20 (8.54%) samples. Only, 140 (59.82%) isolates were sensitive to tigecycline; while 216 isolates were (92.30%) resistant to salbactam. Conclusion: The progressively increasing threat of Acinetobacter resistant infections can be minimized by judicial use of antibiotics, and implementation of strict infection control policy in health care settings.


2020 ◽  
Vol 7 (6) ◽  
pp. 893
Author(s):  
Swarnendu Datta ◽  
Ushnish Chakrabarty ◽  
Priyanka Chakrabarty ◽  
Plaban Mukherjee

Background: Mortality from bacteraemia related to indwelling Central Venous Pressure (CVP) lines could be as high as 12 - 25% and that due to indwelling urinary catheters about 5%, in critically ill patients. Hence, initiation of early and aggressive antibiotic therapy, often even before the culture- sensitivity reports are available is necessary. Objectives of the study was to find out the incidence of bacteraemia  related to indwelling CVP catheters &/or Foley’s catheters in post operative patients in Cardio-thoracic  Intensive Theraputic Unit (ITU) and to understand the antibiotic sensitivity patterns against the organisms causing such bacteraemiaMethods: 48 hours after their insertion, CVP catheter tips, Foley’s catheter tips and Peripheral blood samples were collected for cultures in 50 patients who underwent elective cardiothoracic surgery, over a period of one year and results were interpreted.Results: Incidence of bacteraemia associated with indwelling CVP catheters was 8% and was caused by both - Gram positive (Staphylococcus aureus, Enterococcus faecium) and Gram negative (Acinetobacter baumanii, Pseudomonas aeruginosa) organisms. Foley’s catheter was not related to such blood stream infection.  Vancomycin and Linezolid were effective against the Gram positive organisms; while Piperacillin- Tazobactam, Meropenem, Polymyxin B and Colistin were effective against the Gram negative organisms.Conclusions: Indwelling CVP catheters were associated with blood stream infection in 4 post operative patients in our ITU. An idea about the organisms responsible, as well as their antibiogram may help in early initiation of antibiotics empirically before culture-sensitivity reports are available.


2021 ◽  
Vol 28 (05) ◽  
pp. 671-676
Author(s):  
Mubassir Ullah ◽  
Muhammad Saleh Faisal ◽  
Ali Zaki ◽  
Azam Khan ◽  
Mamoor Khan ◽  
...  

Objective: To determine the frequency of common bacteria and antibiotic sensitivity pattern of ear discharge in patients with chronic otitis media. Study Design: Descriptive Cross Sectional study. Setting: Department of ENT, Northwest General Hospital & Research Centre, Peshawar. Period: February to September 2019. Material & Methods: A total of 196 patients of both gender and age between 13 to 60 years with clinical diagnosis of active chronic otitis media were recruited through consecutive sampling technique. After detailed history and relevant examination, demographic data was recorded and pus specimens were collected from the infected ear on cotton swabs. They were sent to pathology laboratory of hospital for culture of common bacteria (E.coli, Pseudomonas aueroginosa, Proteus mirabilis, Staphylococcus aureus and Klebsiella spp) and their respective antibiotic sensitivity (Co-trimoxazole, Co-Amoxiclav, Ciprofloxacin, Ampicillin, Imipenem, Ceftriaxone and Cefixime). Results: Bacterial isolates were analyzed as 48% Staph aureus, 28% Pseudomonas, 12% Proteus mirabilis, 8% E. coli and 4% Klebsiella spp. Imipenem was sensitive to 94%, ceftriaxone 84%, ciprofloxacin 80%, co-trimoxazole 66%, ampicillin 63%, cefixime 45% and co-amoxiclav against 43% of total bacterial isolates. Conclusion: The common causative organism for active chronic otitis media was Staph aureus followed by Pseudomonas. Imipenem was the most sensitive antibiotic against majority of bacterial isolates followed by ceftriaxone and ciprofloxacin.


2019 ◽  
Vol 17 (2) ◽  
pp. 70-74
Author(s):  
Ankita Singh ◽  
Vikash Verma ◽  
Ritesh Singh

Background: Microbial pathogens cause human skin and soft tissue infections (SSTI) and surgical site infections (SSI) after surgical procedures. These can result in the production of pus, yellowish fluid comprising of dead WBCs and cellular debris. The microorganisms responsible for pus production vary greatly in relation to their spectrum of prevalence in different hospital and also in their antibiotic sensitivity. Further, the antibiotic sensitivity also changes because of the emergence of resistant strains. It is therefore, important that the common bacterial pathogens causing infection in a particular hospital and their sensitivity should be known. This will help in the choice of prophylactic antibiotic and in initiating the empirical antibiotic prescription for the infected cases before the culture sensitivity report is made available which takes about 2-3 days. Objective: To identify the spectrum of aerobic bacteria which are responsible for SSTI and SSI and their antibiotic sensitivity pattern. Method: This cross sectional hospital based study was conducted in Nepalgunj Medical College and Teaching Hospital (NGMCTH), Kohalpur from January 2019 to November 2019. These pus swabs were obtained from the Department of Surgery and Department of Gynaecology & Obstretics. Samples were cultured in the Microbiology laboratory of NGMCTH, Kohalpur. Identification and characterization of isolates were performed on the basis of Gram staining and cultural characteristics. Antibiotic sensitivity test was performed in vitro by Bauer-Kirby method. Collected data were statistically analyzed using SPSS 20.0 and Microsoft Excel 2015. Results: During the study period, a total number of 311 pus swabs were obtained among which only 164 (52.73%) pus swab showed bacterial growth. Out of 164 pus swabs, 150 pus swabs yielded monomicrobial growth (150 bacterial isolates) and 14 pus swabs yielded polymicrobial growth (33 bacterial isolates). Gram Negative Bacteria (60.1%) was more prevalent than Gram Positive Bacteria (39.9%). Combined together, the most common isolate was S. aureus (36.1%) followed by E.coli (24.0%), Klebsiella (14.2%), Enterobacter (11.5%), Pseudomonas (9.8%), S. pyogenes (3.3%) and Proteus (1.1%). S. aureus was highly sensitive to Doxycycline (90.6%), Chloramphenicol (81.5%), Amikacin (79.5%) and Ceftraixone (72.7%). S. pyogenes showed 100% sensitivity to Cefexime, Amikacin, Chloramphenicol, Azithromycin and 80% sensitivity to Doxycycline. Similarly, most common gram negative isolate E.coli showed higher sensitivity to Chloramphenicol(71.4%) and Amikacin (66.7%) , Klebsiella showed higher sensitivity to Doxycycline(92.3%), Gentamicin(87.5%) and Amikacin (81.0%), Enterobacter showed higher sensitivity to Amikacin(90.9%) and Pseudomonas was highly sensitive to Chloramphenicol (71.4%) and Amikacin (66.7%). Piperacillin, Amikacin, Gentamicin, Ofloxacin and Ceftriaxone showed 100% sensitivity to Proteus spp. Amoxyclav, Cefepime and Cefexime (except in S. pyogenes) showed least sensitivity in both gram negative and gram positive bacterial isolates. Conclusion: In our study, the most common isolate wasS. aureus. Amikacin, as a single drug was found to be effective for empirical therapy of both gram negative and gram positive bacteria whereas Doxycycline and Amikacin was found effective in gram positive isolates. Amoxyclav and Cefepimewas commonly resistant in all bacterial isolates.


2021 ◽  
pp. 1-3
Author(s):  
A.V. Kavitha ◽  
Thyagarajan Ravinder ◽  
Radhika Katragadda ◽  
Leela Vajravelu

Introduction: Chronic Suppurative Otitis Media (CSOM) is one of the major cause of preventable hearing loss if treated promptly. Emergence of resistant strains in the world is of great concern. The aim of the present study was to determine the aetiology and antibiotic sensitivity pattern of bacterial isolates from CSOM cases with special emphasis on Methicillin Resistant Staphylococcus aureus. Materials and methods: Ear swabs were collected from 212 patients attending otorhinolaryngology department, GKMC, Chennai was processed in Microbiology lab. Direct gram staining was done and then inoculated into Blood, MacConkey and Nutrient agar. Bacterial identification was done using standard microbiological techniques. Antibiotic susceptibility was done by Kirby Bauer disc diffusion technique. Methicillin Resistant Staphylococcus aureus (MRSA) was detected by Cefoxitin disc diffusion method as per CLSI guidelines. Results: 60 Gram positive bacteria were isolated from collected ear swabs. Staphylococcus aureus 46(26.59%) was the most common bacteria among Gram positive organism followed by CONS 9(5.20%) and Enterococci 5(2.89%). Staphylococcus aureus showed 41(89.13%) was sensitive to amikacin and 40(86.95%) to ofloxacin. All the Gram Positive cocci were 100% sensitive to Vancomycin and Linezolid. 19.53% were confirmed as MRSA by cefoxitin disc diffusion method. Conclusion: Microbial pattern can vary in different location in different periods of time. Hence knowledge of appropriate aetiology and antimicrobial resistance pattern of CSOM helps in rational use of antibiotics and control of drug resistance.MRSA is emerging as an important pathogen in CSOM. Empirical antibiotics should be directed to gram positives, and especially, MRSA should be taken into consideration.


2020 ◽  
Vol 14 (3) ◽  
pp. 1
Author(s):  
Mardhia Mardhia ◽  
Mahyarudin Mahyarudin ◽  
Abror Irsan

Diabetic patients are associated with a higher risk of infection. The research purposed to identify antibiotic susceptibility patterns among diabetic outpatients with urinary tract infection in Pontianak. An experimental study was performed for 13 bacterial isolates of diabetic outpatients with urinary tract infection in the Clinic of Diabetes Mellitus, Sultan Syarif Mohamad Alkadrie Hospital, Pontianak. The disc diffusion method was used to perform the susceptibility of antibiotics to the bacterial isolates. Among 13 isolates, the most common causative agent of urinary tract infection was Escherichia coli (53.85%), followed by Pseudomonas aeruginosa (30.77%). Klebsiella spp and Enterobacter aerogenes were 7.69%. Most isolates of bacteria of the study had a high sensitivity to Cefepime (92.31%), then followed by Levofloxacin, Amikacin, and Meropenem for 84.62%. The study revealed low sensitivity of bacteria to Amoxicillin/Clavulanate, Co-Trimoxazole, Cefazoline and Ceftriaxone (30.77%, 23.08 %, 23.08%, 23.08%, respectively). All bacterial isolates had high resistance to Ampicillin. Moreover, multidrug resistance observed among bacterial isolates. Keywords: antibiotic susceptibility, diabetes, urinary tract infections


2020 ◽  
Vol 3 (02) ◽  
pp. 30-34
Author(s):  
Md. Bani Yeamin ◽  
Mst. Nazma Khatun ◽  
Lutfor Rahman Mollah ◽  
Rezanur Rahman ◽  
Mohammad Tanvir Ahamed ◽  
...  

Background: Acute lymphoblastic leukemia (ALL) is the commonest malignancies in childhood. Common obstacle in the treatment of ALL is febrile neutropenia and its complications. Objectives: To identify bacteria causing infection, their isolation rate and antibacterial sensitivity pattern in hospitalized febrile neutropenic children with ALL in different cycle of chemotherapy. Methodology: This observational study conducted in 2014 - 2015 in the department of paediatric haematology and oncology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka Bangladesh. Sixty febrile neutropenic episodes from 52 diagnosed cases of ALL aged 0 to18 years were included. Complete blood count, blood culture, urine microscopy and culture, serum alanine aminotransferase, serum creatinine were done in every patient. X-ray chest, stool microscopy and culture, pus, wound, throat and aural swab for culture & sensitivity were done in selective patient. Results: Bacterial infection was confirmed by culture in 15 (25%) episodes from 60 febrile neutropenic episodes. Fifteen (25%) organisms were isolated from the study subjects from sample of blood (60%), pus (13.3%), aural swab (13.3%), wound swab (6.7%) and throat swab (6.7%) respectively. All isolates were gram negative. The organism isolated were Klebsiella spp. 5 (33.31%), E. coli 4 (26.7%), Acinetobacter 3 (20%), Pseudomonas 2 (13.3%) and only one (6.7%) Enterobacter species. All the isolates of the Klebsiella spp., E. coli and Acinetobacter spp. were resistant to amoxicillin. All isolated E. coli were resistant to cotrimoxazole, ceftazidime, ceftriaxone, cefotaxime and ciprofloxacin, Acinetobacter spp. Isolated were 100% sensitive to imipenem, colistin sulphate & piperacillin-tazobactam and resistant to cotrimoxazole and cephradine. All Pseudomonas spp. showed 100% sensitivity to imipenem, amikacin, ciprofloxacin & colistin and resistances to ceftazidime. Conclusion: The species of Klebsiella were the predominant causative bacterial agent followed by Escherichia coli, Acinetobacter spp, pseudomonas spp. and Enterobacter spp. They showed resistance to commonly prescribed antibiotics ceftazidime, gentamicin, ceftriaxone & ciprofloxacin and sensitive to imipenem, colistin-sulphate & piperacillin-tazobactam.


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